Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,005,219 T→C V584A (GTT→GCT)  recQ → ATP‑dependent DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,005,2190TC99.3% 1459.4 / ‑3.9 453V584A (GTT→GCT) recQATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base C (220/230);  total (221/232)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

Only 100 of 471 total aligned reads displayed.

CGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTCAGCAG  >  NC_000913/4005129‑4005308
                                                                                          |                                                                                         
  ccgTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTggg                                                                                       >  2:2089031/1‑93 (MQ=255)
   cgTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGa                                                                                      <  2:9925438/93‑1 (MQ=255)
   cgTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGa                                                                                      <  2:4935981/93‑1 (MQ=255)
   cgTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGa                                                                                      <  1:1598507/93‑1 (MQ=255)
     tACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATg                                                                                    <  2:3477303/93‑1 (MQ=255)
     tACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATg                                                                                    >  1:6271784/1‑93 (MQ=255)
        gtggtgTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATgcgc                                                                                 <  2:6813340/93‑1 (MQ=255)
        gtggtgTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATgcgc                                                                                 >  1:3851590/1‑93 (MQ=255)
        gtggtgTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATgcgc                                                                                 >  1:9460979/1‑93 (MQ=255)
        gtggtgTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATgcgc                                                                                 >  1:3119122/1‑93 (MQ=255)
         tggtgTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCa                                                                                >  2:969788/1‑93 (MQ=255)
         tggtgTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCa                                                                                >  1:5627245/1‑93 (MQ=255)
          ggtgTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCag                                                                               >  2:2901821/1‑92 (MQ=255)
          ggtgTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCaa                                                                               >  2:9619509/1‑93 (MQ=255)
           gtgtTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAg                                                                              >  1:8875104/1‑93 (MQ=255)
              tttAACGAGGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTg                                                                           >  1:4692997/1‑93 (MQ=255)
              tttAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTg                                                                           >  1:9158248/1‑93 (MQ=255)
              tttAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTg                                                                           <  1:9105945/93‑1 (MQ=255)
               ttAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTgg                                                                          <  1:3770616/93‑1 (MQ=255)
                 aaCGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGaa                                                                        <  2:1296664/93‑1 (MQ=255)
                 aaCGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGaa                                                                        <  2:9548534/93‑1 (MQ=255)
                  acgacgCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAAc                                                                       >  2:1552368/1‑93 (MQ=255)
                    gacgCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGc                                                                     >  1:1809520/1‑93 (MQ=255)
                    gacgCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGc                                                                     <  2:3771622/93‑1 (MQ=255)
                     acgCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCt                                                                    >  1:1988405/1‑93 (MQ=255)
                      cgCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCtt                                                                   <  2:239312/93‑1 (MQ=255)
                      cgCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCtt                                                                   >  2:4062809/1‑93 (MQ=255)
                       gCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCttt                                                                  >  2:7055604/1‑93 (MQ=255)
                        cAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTg                                                                 >  1:8613677/1‑93 (MQ=255)
                        cAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTg                                                                 <  2:440083/93‑1 (MQ=255)
                        cAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTg                                                                 <  2:751369/93‑1 (MQ=255)
                        cAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTg                                                                 >  1:3195740/1‑93 (MQ=255)
                         aaCCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTgg                                                                <  1:4208945/93‑1 (MQ=255)
                          aCCTTGATTGAGATGGCTGAACGGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTCGGc                                                               <  2:8643796/93‑1 (MQ=255)
                          aCCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGc                                                               >  2:7876596/1‑93 (MQ=255)
                            cTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCaa                                                             <  2:8201709/93‑1 (MQ=255)
                            cTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCaa                                                             <  1:1321047/93‑1 (MQ=255)
                             ttgattgaGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCaaa                                                            >  1:7764444/1‑93 (MQ=255)
                             ttgattgaGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCaaa                                                            <  1:1534836/93‑1 (MQ=255)
                              tgattgaGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAAc                                                           >  2:2234773/1‑93 (MQ=255)
                              tgattgaGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAAc                                                           <  1:10026201/93‑1 (MQ=255)
                                 ttgaGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGt                                                        <  2:5703368/93‑1 (MQ=255)
                                  tgaGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGtt                                                       <  1:995891/93‑1 (MQ=255)
                                    agaTGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTa                                                     <  1:8495144/93‑1 (MQ=255)
                                    agaTGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTa                                                     >  2:2935342/1‑93 (MQ=255)
                                    agaTGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTa                                                     <  1:9791778/93‑1 (MQ=255)
                                     gaTGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTAt                                                    >  2:4845214/1‑93 (MQ=255)
                                        ggCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGgc                                                 >  2:8457754/1‑93 (MQ=255)
                                           tGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCt                                              >  1:7047315/1‑93 (MQ=255)
                                            gAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTg                                             >  1:3949268/1‑93 (MQ=255)
                                            gAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTg                                             >  1:5190042/1‑93 (MQ=255)
                                             aaCAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGa                                            <  2:6145951/93‑1 (MQ=255)
                                               cAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGAtt                                          <  2:3598151/93‑1 (MQ=255)
                                               cAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGAtt                                          <  1:6947187/93‑1 (MQ=255)
                                                aGATGCCGATCACCGCCAGCGAAATGCTCACCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGATTTGGCAAACCGTTTATGGCGCTGATTc                                         >  1:9609462/1‑93 (MQ=255)
                                                 gATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCg                                        <  1:8008440/93‑1 (MQ=255)
                                                  aTGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGt                                       <  1:590587/93‑1 (MQ=255)
                                                   tGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTg                                      <  2:4216677/93‑1 (MQ=255)
                                                    gCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTgc                                     <  2:5448510/93‑1 (MQ=255)
                                                        aTCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCAt                                 >  1:6149030/1‑93 (MQ=255)
                                                         tCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATg                                <  1:8215739/93‑1 (MQ=255)
                                                         tCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATg                                <  1:5420006/93‑1 (MQ=255)
                                                          cACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGt                               >  2:8824873/1‑93 (MQ=255)
                                                          cACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGt                               <  1:3498247/93‑1 (MQ=255)
                                                           aCCGCCAGCAAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGtt                              >  2:6678152/1‑93 (MQ=255)
                                                            ccGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTg                             >  2:8116637/1‑93 (MQ=255)
                                                             ccccAGCGAAATTCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGa                            <  1:483641/91‑1 (MQ=255)
                                                             cGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGa                            <  1:5123243/93‑1 (MQ=255)
                                                             cGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGa                            <  1:3411844/93‑1 (MQ=255)
                                                              gCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGAt                           <  2:8372300/93‑1 (MQ=255)
                                                                cAGCGAAATGCTCAGCGTTAACGGCGCTGGCATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATgg                         <  1:6528132/93‑1 (MQ=255)
                                                                 aGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGc                        <  1:7410975/93‑1 (MQ=255)
                                                                 aGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGc                        >  2:5812979/1‑93 (MQ=255)
                                                                   cGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGa                      <  2:7789094/93‑1 (MQ=255)
                                                                    gAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGAt                     <  2:1190329/93‑1 (MQ=255)
                                                                    gAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGAt                     >  2:9376374/1‑93 (MQ=255)
                                                                     aaaTGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATg                    >  1:6409883/1‑93 (MQ=255)
                                                                      aaTGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGa                   <  1:577052/93‑1 (MQ=255)
                                                                      aaTGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGa                   <  2:592465/93‑1 (MQ=255)
                                                                       aTGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGAc                  >  2:3185327/1‑93 (MQ=255)
                                                                         gCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGa                <  2:5176725/93‑1 (MQ=255)
                                                                          cTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGaa               >  1:4463059/1‑93 (MQ=255)
                                                                          cTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGaa               <  1:7618867/93‑1 (MQ=255)
                                                                          cTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGaa               <  1:2976741/93‑1 (MQ=255)
                                                                            cAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAaga             <  1:2320807/93‑1 (MQ=255)
                                                                            cAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAaga             <  1:2512166/93‑1 (MQ=255)
                                                                             aGCCTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAagag            <  2:2778798/93‑1 (MQ=255)
                                                                              gCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGagt           <  2:7141033/93‑1 (MQ=255)
                                                                               cGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGagta          <  2:2621740/93‑1 (MQ=255)
                                                                                gTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGagtag         >  1:3669127/1‑93 (MQ=255)
                                                                                 ttAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGagtagt        <  1:8721482/93‑1 (MQ=255)
                                                                                  tAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTc       <  1:1086108/93‑1 (MQ=255)
                                                                                  tAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTc       <  2:1871958/93‑1 (MQ=255)
                                                                                   accGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTTATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTca      <  2:7338213/91‑1 (MQ=255)
                                                                                   aaCGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTca      <  1:4315023/93‑1 (MQ=255)
                                                                                    aCGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTcag     <  1:2708129/93‑1 (MQ=255)
                                                                                      ggCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTcagca   <  2:386946/93‑1 (MQ=255)
                                                                                      ggCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTcagca   <  2:4617930/93‑1 (MQ=255)
                                                                                      ggCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTcagca   <  2:6564570/93‑1 (MQ=255)
                                                                                       gcgcTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTcagcag  <  2:9083009/93‑1 (MQ=255)
                                                                                          |                                                                                         
CGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTCAGCAG  >  NC_000913/4005129‑4005308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 250 total aligned reads displayed.
TCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTCAGCAGCATA  >  NC_000913/4005126‑4005312
                                                                                             |                                                                                             
TCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGC                                                                                               >  SRR4302540.9256692/1‑94 (MQ=60)
 CCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCT                                                                                              >  SRR4302540.843413/1‑94 (MQ=60)
   CGCCGTACGTGGTGTTCAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGAGTTAACGGCGCTGG                                                                                            >  SRR4302540.6178204/1‑94 (MQ=60)
    GCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGG                                                                                           <  SRR4302540.8644796/94‑1 (MQ=60)
    GCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGG                                                                                           <  SRR4302540.924170/94‑1 (MQ=60)
      CGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGAT                                                                                         >  SRR4302540.4964492/1‑94 (MQ=60)
      CGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGAT                                                                                         >  SRR4302540.7104984/1‑94 (MQ=60)
      CGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGAT                                                                                         <  SRR4302540.1615985/94‑1 (MQ=60)
      CGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGAT                                                                                         <  SRR4302540.3667087/94‑1 (MQ=60)
       GTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATG                                                                                        >  SRR4302540.2538371/1‑94 (MQ=60)
       GTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATG                                                                                        >  SRR4302540.7787407/1‑94 (MQ=60)
        TACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGC                                                                                       >  SRR4302540.5847148/1‑94 (MQ=60)
        TACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGC                                                                                       <  SRR4302540.8632032/94‑1 (MQ=60)
        TACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGC                                                                                       >  SRR4302540.9867933/1‑94 (MQ=60)
         ACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCG                                                                                      >  SRR4302540.1620999/1‑94 (MQ=60)
          CGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGC                                                                                     >  SRR4302540.3893467/1‑94 (MQ=60)
          CGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGC                                                                                     >  SRR4302540.9573234/1‑94 (MQ=60)
           GTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCA                                                                                    >  SRR4302540.5689184/1‑94 (MQ=60)
           GTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCA                                                                                    >  SRR4302540.9192534/1‑94 (MQ=60)
           GTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCA                                                                                    >  SRR4302540.7436907/1‑94 (MQ=60)
            TGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAA                                                                                   <  SRR4302540.1374169/94‑1 (MQ=60)
            TGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAA                                                                                   >  SRR4302540.5239101/1‑94 (MQ=60)
             GGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAG                                                                                  >  SRR4302540.8977816/1‑94 (MQ=60)
             GGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAG                                                                                  >  SRR4302540.6320785/1‑94 (MQ=60)
             GGGGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAG                                                                                  <  SRR4302540.8240842/94‑1 (MQ=60)
              GTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGT                                                                                 >  SRR4302540.5796278/1‑94 (MQ=60)
              GTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGC                                                                                 <  SRR4302540.5814019/94‑1 (MQ=60)
              GTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGC                                                                                 <  SRR4302540.3778923/94‑1 (MQ=60)
              GTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGC                                                                                 >  SRR4302540.6597913/1‑94 (MQ=60)
              GTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGACCAGCGTTAACGGCGATGGGATGCGAAAGC                                                                                 >  SRR4302540.7841020/1‑94 (MQ=60)
               TGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCT                                                                                <  SRR4302540.2883693/94‑1 (MQ=60)
               TGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCT                                                                                >  SRR4302540.4412351/1‑94 (MQ=60)
                GTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTG                                                                               >  SRR4302540.9268180/1‑94 (MQ=60)
                 TTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGGTGCGCAAGCTGG                                                                              <  SRR4302540.6115402/94‑1 (MQ=60)
                 TTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGG                                                                              <  SRR4302540.4136517/94‑1 (MQ=60)
                 TTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGG                                                                              >  SRR4302540.1631938/1‑94 (MQ=60)
                  TTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGA                                                                             <  SRR4302540.6304537/94‑1 (MQ=60)
                  TTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGA                                                                             <  SRR4302540.8852406/94‑1 (MQ=60)
                    AACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAAC                                                                           <  SRR4302540.9438537/94‑1 (MQ=60)
                    AACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAAC                                                                           <  SRR4302540.5660467/94‑1 (MQ=60)
                    AACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAAC                                                                           <  SRR4302540.135532/94‑1 (MQ=60)
                     ACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACG                                                                          <  SRR4302540.5121320/94‑1 (MQ=60)
                     ACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACG                                                                          >  SRR4302540.323583/1‑94 (MQ=60)
                     ACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACG                                                                          >  SRR4302540.1847285/1‑94 (MQ=60)
                     ACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACG                                                                          >  SRR4302540.1847284/1‑94 (MQ=60)
                      CGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGC                                                                         >  SRR4302540.1829245/1‑94 (MQ=60)
                       GACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCT                                                                        >  SRR4302540.2009952/1‑94 (MQ=60)
                         CGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTT                                                                      <  SRR4302540.155972/94‑1 (MQ=60)
                         CGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTT                                                                      <  SRR4302540.9224557/94‑1 (MQ=60)
                         CGCAACCTTGATTGAGATGGCTGAACAGATGCCGATAACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGCGATGCGCAAGCTGGAACGCTTT                                                                      >  SRR4302540.7027061/1‑94 (MQ=60)
                          GCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTG                                                                     >  SRR4302540.8713761/1‑94 (MQ=60)
                            AACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGC                                                                   <  SRR4302540.4254766/94‑1 (MQ=60)
                              CCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAA                                                                 >  SRR4302540.8465661/1‑94 (MQ=60)
                               CTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAA                                                                >  SRR4302540.7855210/1‑94 (MQ=60)
                               CTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAA                                                                <  SRR4302540.1334811/94‑1 (MQ=60)
                               CTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAA                                                                <  SRR4302540.1334810/94‑1 (MQ=60)
                                TTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAAC                                                               <  SRR4302540.1551816/94‑1 (MQ=60)
                                TTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTCGGCAAAC                                                               >  SRR4302540.3229997/1‑94 (MQ=60)
                                 TGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACC                                                              <  SRR4302540.3870228/94‑1 (MQ=60)
                                 TGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACC                                                              >  SRR4302540.3834046/1‑94 (MQ=60)
                                 TGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACC                                                              <  SRR4302540.10142863/94‑1 (MQ=60)
                                 TGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACC                                                              >  SRR4302540.2301856/1‑94 (MQ=60)
                                 TGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACC                                                              >  SRR4302540.440689/1‑94 (MQ=60)
                                    TTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTT                                                           <  SRR4302540.8030541/94‑1 (MQ=60)
                                     TGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTT                                                          <  SRR4302540.1006031/94‑1 (MQ=60)
                                     TGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTT                                                          >  SRR4302540.9880204/1‑94 (MQ=60)
                                     TGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTT                                                          <  SRR4302540.601017/94‑1 (MQ=60)
                                     TGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTT                                                          <  SRR4302540.8846132/94‑1 (MQ=60)
                                       AGATGGCTGAACAGATGGCGATCACCGCCAGCGAAATGCTCAGCTTTAACGCCGCTAGGTTGCCAAAGCTGGAACGCTTTGGCAAACCGTTcag                                                        >  SRR4302540.8063138/1‑91 (MQ=59)
                                       AGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTAT                                                        <  SRR4302540.8593858/94‑1 (MQ=60)
                                       AGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTAT                                                        >  SRR4302540.5234147/1‑94 (MQ=60)
                                       AGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTAT                                                        >  SRR4302540.8063137/1‑94 (MQ=60)
                                       AGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTAT                                                        <  SRR4302540.4918211/94‑1 (MQ=60)
                                       AGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTAT                                                        >  SRR4302540.880620/1‑94 (MQ=60)
                                       AGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTAT                                                        <  SRR4302540.8988385/94‑1 (MQ=60)
                                       AGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTAT                                                        <  SRR4302540.5934735/94‑1 (MQ=60)
                                       AGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTAT                                                        <  SRR4302540.6188692/94‑1 (MQ=60)
                                       AGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTAT                                                        <  SRR4302540.3501563/94‑1 (MQ=60)
                                       AGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTAT                                                        >  SRR4302540.1421703/1‑94 (MQ=60)
                                       AGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTAT                                                        <  SRR4302540.251980/94‑1 (MQ=60)
                                        GATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATG                                                       <  SRR4302540.536852/94‑1 (MQ=60)
                                        GATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATG                                                       <  SRR4302540.657720/94‑1 (MQ=60)
                                        GATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATG                                                       <  SRR4302540.2746229/94‑1 (MQ=60)
                                         ATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGG                                                      >  SRR4302540.1184299/1‑94 (MQ=60)
                                          TGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGC                                                     >  SRR4302540.9433021/1‑94 (MQ=60)
                                          TGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTAGGCAAACCGTTTATGca                                                     >  SRR4302540.3500265/1‑92 (MQ=60)
                                           GGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCG                                                    <  SRR4302540.5886794/94‑1 (MQ=60)
                                            GCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGC                                                   >  SRR4302540.7239897/1‑94 (MQ=60)
                                            GCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGC                                                   <  SRR4302540.8733442/94‑1 (MQ=60)
                                             CTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCT                                                  >  SRR4302540.7125687/1‑94 (MQ=60)
                                              TGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTG                                                 >  SRR4302540.172083/1‑94 (MQ=60)
                                              TGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTG                                                 <  SRR4302540.4067547/94‑1 (MQ=60)
                                              TGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTG                                                 >  SRR4302540.5246544/1‑94 (MQ=60)
                                              TGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTG                                                 >  SRR4302540.34141/1‑94 (MQ=60)
                                              TGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTG                                                 >  SRR4302540.10035095/1‑94 (MQ=60)
                                              TGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTG                                                 >  SRR4302540.9149657/1‑94 (MQ=60)
                                               GAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGT                                                >  SRR4302540.3476977/1‑94 (MQ=60)
                                                AACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGAT                                               >  SRR4302540.4013282/1‑94 (MQ=60)
                                                 ACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATT                                              >  SRR4302540.4337787/1‑94 (MQ=60)
                                                 ACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATT                                              >  SRR4302540.5302249/1‑94 (MQ=60)
                                                  CAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTC                                             >  SRR4302540.3149830/1‑94 (MQ=60)
                                                  CAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTC                                             <  SRR4302540.959165/94‑1 (MQ=60)
                                                  CAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTC                                             <  SRR4302540.8279955/94‑1 (MQ=60)
                                                  CAGATGCCGATCACCGCCAGCGAAATGCTCACCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGATTTGGCAAACCGTTTATGGCGCTGATTC                                             >  SRR4302540.9722773/1‑94 (MQ=60)
                                                   AGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCG                                            <  SRR4302540.7115364/94‑1 (MQ=60)
                                                   AGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCG                                            >  SRR4302540.3176951/1‑94 (MQ=60)
                                                   AGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCG                                            >  SRR4302540.3226752/1‑94 (MQ=60)
                                                   AGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCG                                            >  SRR4302540.10042931/1‑94 (MQ=60)
                                                   AGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCG                                            >  SRR4302540.3107589/1‑94 (MQ=60)
                                                    GATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGT                                           <  SRR4302540.6805315/94‑1 (MQ=60)
                                                    GATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGT                                           <  SRR4302540.8101618/94‑1 (MQ=60)
                                                     ATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTG                                          >  SRR4302540.3233274/1‑94 (MQ=60)
                                                      TGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGC                                         >  SRR4302540.10069014/1‑94 (MQ=60)
                                                       GCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCG                                        <  SRR4302540.238956/94‑1 (MQ=60)
                                                        CCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGC                                       >  SRR4302540.7635954/1‑94 (MQ=60)
                                                        CCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGC                                       >  SRR4302540.6449815/1‑94 (MQ=60)
                                                          GATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCAT                                     >  SRR4302540.5382720/1‑94 (MQ=60)
                                                          GATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCAT                                     <  SRR4302540.3763644/94‑1 (MQ=60)
                                                          GATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCAT                                     >  SRR4302540.8282509/1‑94 (MQ=60)
                                                           ATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATG                                    <  SRR4302540.4516637/94‑1 (MQ=60)
                                                           ATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATG                                    <  SRR4302540.3198205/94‑1 (MQ=60)
                                                           ATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATG                                    >  SRR4302540.4666682/1‑94 (MQ=60)
                                                           ATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATG                                    <  SRR4302540.7668573/94‑1 (MQ=60)
                                                            TCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGT                                   <  SRR4302540.5479602/94‑1 (MQ=60)
                                                            TCACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGT                                   <  SRR4302540.8311229/94‑1 (MQ=60)
                                                             CCCCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTT                                  <  SRR4302540.5876136/94‑1 (MQ=60)
                                                             CACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTT                                  <  SRR4302540.3536343/94‑1 (MQ=60)
                                                             CACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTT                                  >  SRR4302540.2261689/1‑94 (MQ=60)
                                                              ACCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTG                                 <  SRR4302540.4346508/94‑1 (MQ=60)
                                                               CCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGA                                <  SRR4302540.8739550/94‑1 (MQ=60)
                                                               CCGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGA                                <  SRR4302540.326669/94‑1 (MQ=60)
                                                                CTCCAGCGAAATGCTAAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGAT                               <  SRR4302540.5241488/94‑1 (MQ=60)
                                                                CGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGAT                               <  SRR4302540.2396555/94‑1 (MQ=60)
                                                                CGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGAT                               <  SRR4302540.6803490/94‑1 (MQ=60)
                                                                CGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGAT                               <  SRR4302540.7621916/94‑1 (MQ=60)
                                                                CGCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGAT                               <  SRR4302540.4785521/94‑1 (MQ=60)
                                                                 GCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATG                              <  SRR4302540.1016018/94‑1 (MQ=60)
                                                                 GCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATG                              <  SRR4302540.7148512/94‑1 (MQ=60)
                                                                 GCCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATG                              <  SRR4302540.3825975/94‑1 (MQ=60)
                                                                  CCAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGG                             >  SRR4302540.2733446/1‑94 (MQ=60)
                                                                   CAGCGAAATGCTCAGCGTTAACGGCTCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGC                            <  SRR4302540.6762837/94‑1 (MQ=60)
                                                                   CAGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGC                            <  SRR4302540.6762831/94‑1 (MQ=60)
                                                                    AGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCG                           <  SRR4302540.7493522/94‑1 (MQ=60)
                                                                    AGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCG                           >  SRR4302540.2583902/1‑94 (MQ=60)
                                                                    AGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCG                           >  SRR4302540.5611199/1‑94 (MQ=60)
                                                                    AGCGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCG                           >  SRR4302540.369292/1‑94 (MQ=60)
                                                                      CGAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGAT                         >  SRR4302540.6218297/1‑94 (MQ=60)
                                                                       GAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATG                        <  SRR4302540.3990924/94‑1 (MQ=60)
                                                                       GAAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATG                        >  SRR4302540.6481223/1‑94 (MQ=60)
                                                                        AAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGA                       >  SRR4302540.3953894/1‑94 (MQ=60)
                                                                        AAATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGA                       >  SRR4302540.3981432/1‑94 (MQ=60)
                                                                         AATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGAC                      <  SRR4302540.1245992/94‑1 (MQ=60)
                                                                          ATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTCTGGCGCTGATTCGTGCGCATGTTGATGGCGATGACG                     >  SRR4302540.304155/1‑94 (MQ=60)
                                                                          ATGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACG                     <  SRR4302540.9665950/94‑1 (MQ=60)
                                                                           TGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGA                    <  SRR4302540.4420638/94‑1 (MQ=60)
                                                                           TGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGA                    <  SRR4302540.5832122/94‑1 (MQ=60)
                                                                           TGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGA                    <  SRR4302540.1572606/94‑1 (MQ=60)
                                                                           TGCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGA                    <  SRR4302540.6509229/94‑1 (MQ=60)
                                                                            GCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAA                   <  SRR4302540.10100047/94‑1 (MQ=60)
                                                                            GCTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAA                   <  SRR4302540.461768/94‑1 (MQ=60)
                                                                             CTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAG                  <  SRR4302540.3009636/94‑1 (MQ=60)
                                                                             CTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAG                  >  SRR4302540.6373575/1‑94 (MQ=60)
                                                                             CTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAG                  >  SRR4302540.9663990/1‑94 (MQ=60)
                                                                             CTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAG                  <  SRR4302540.4784340/94‑1 (MQ=60)
                                                                             CTCAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAG                  <  SRR4302540.7704536/94‑1 (MQ=60)
                                                                               CAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAG                <  SRR4302540.2539301/94‑1 (MQ=60)
                                                                               CAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAG                <  SRR4302540.1831623/94‑1 (MQ=60)
                                                                               CAGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAG                <  SRR4302540.3727797/94‑1 (MQ=60)
                                                                                AGCGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGT               <  SRR4302540.8899054/94‑1 (MQ=60)
                                                                                  CGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAG             <  SRR4302540.9218994/94‑1 (MQ=60)
                                                                                  CGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAG             >  SRR4302540.9283077/1‑94 (MQ=60)
                                                                                  CGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAG             <  SRR4302540.4317685/94‑1 (MQ=60)
                                                                                  CGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAG             <  SRR4302540.2639873/94‑1 (MQ=60)
                                                                                  CGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAG             <  SRR4302540.9817825/94‑1 (MQ=60)
                                                                                  CGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAG             <  SRR4302540.4775573/94‑1 (MQ=60)
                                                                                  CGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAG             <  SRR4302540.4780667/94‑1 (MQ=60)
                                                                                  CGTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAG             >  SRR4302540.3709263/1‑94 (MQ=60)
                                                                                   GTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGT            >  SRR4302540.4695143/1‑94 (MQ=60)
                                                                                   GTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGT            >  SRR4302540.645921/1‑94 (MQ=60)
                                                                                   GTTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGAAGT            >  SRR4302540.2040362/1‑94 (MQ=60)
                                                                                    TTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTC           <  SRR4302540.8822750/94‑1 (MQ=60)
                                                                                    GTAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTC           <  SRR4302540.10145223/94‑1 (MQ=60)
                                                                                     TAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTCA          >  SRR4302540.3675989/1‑94 (MQ=60)
                                                                                     TAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTCA          <  SRR4302540.1097239/94‑1 (MQ=60)
                                                                                     TAACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCCGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTCA          <  SRR4302540.2851375/94‑1 (MQ=60)
                                                                                      AACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTCAG         <  SRR4302540.4362003/94‑1 (MQ=60)
                                                                                       ACGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTCAGC        >  SRR4302540.5119822/1‑94 (MQ=60)
                                                                                        CGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTCAGCA       <  SRR4302540.851909/94‑1 (MQ=60)
                                                                                        CGGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTCAGCA       <  SRR4302540.632745/94‑1 (MQ=60)
                                                                                         GGCGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTCAGCAG      <  SRR4302540.8440701/94‑1 (MQ=60)
                                                                                           CGCTGGGATGCGCAAGCTGTAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTCAGCAGCA    >  SRR4302540.3873716/1‑94 (MQ=60)
                                                                                           CGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACTGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTCAGCAGCA    <  SRR4302540.6513588/94‑1 (MQ=60)
                                                                                           CGCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTCAGCAGCA    <  SRR4302540.4067325/94‑1 (MQ=60)
                                                                                            GCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTCAGCAGCAT   >  SRR4302540.7845340/1‑94 (MQ=60)
                                                                                            GCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTCAGCAGCAT   <  SRR4302540.1254546/94‑1 (MQ=60)
                                                                                            GCTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTCAGCAGCAT   <  SRR4302540.8657605/94‑1 (MQ=60)
                                                                                             CTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTCAGCAGCATA  >  SRR4302540.2939910/1‑94 (MQ=60)
                                                                                             CTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTCAGCAGCATA  >  SRR4302540.5655850/1‑94 (MQ=60)
                                                                                             CTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTCAGCAGCATA  >  SRR4302540.3798556/1‑94 (MQ=60)
                                                                                             CTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTCAGCAGCATA  >  SRR4302540.5655851/1‑94 (MQ=60)
                                                                                             |                                                                                             
TCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTCAGCAGCATA  >  NC_000913/4005126‑4005312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑