Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,085,675	Δ1 bp	intergenic (+38/+13)	yiiG → / ← frvR	hypothetical protein/putative frv operon regulatory protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,085,675	0	G	.	99.4%	2148.6 / 2.2	483	intergenic (+38/+13)	yiiG/frvR	hypothetical protein/putative frv operon regulatory protein
Reads supporting (aligned to +/- strand): ref base G (1/0); major base . (291/188); minor base C (0/3); total (292/191)
Fisher's exact test for biased strand distribution p-value = 6.16e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 526 total aligned reads displayed.

GCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCGATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACA  >  NC_000913/4085587‑4085762

 cATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGa                                                                                   >  1:2827645/1‑93 (MQ=255)

   ttACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGaca                                                                                 <  1:639687/93‑1 (MQ=255)

    tACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGacac                                                                                >  1:7660321/1‑93 (MQ=255)

     aCGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGAcaca                                                                               <  1:7592457/93‑1 (MQ=255)

     aCGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGAcaca                                                                               <  1:4716805/93‑1 (MQ=255)

      cgcgAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAg                                                                              >  2:272018/1‑93 (MQ=255)

       gcgAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGt                                                                             >  1:7387469/1‑93 (MQ=255)

        cgAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGtt                                                                            >  2:8846437/1‑93 (MQ=255)

         gAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCTGGTGGGGCG‑GATGCGACACAGGTc                                                                           >  2:5429576/1‑93 (MQ=255)

         gAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTc                                                                           >  1:2450025/1‑93 (MQ=255)

          aGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCa                                                                          >  1:9993396/1‑93 (MQ=255)

          aGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCa                                                                          <  1:6694557/93‑1 (MQ=255)

          aGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCa                                                                          >  1:1117540/1‑93 (MQ=255)

            tACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCAcc                                                                        <  2:9685492/93‑1 (MQ=255)

            tACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCAcc                                                                        >  2:8073614/1‑93 (MQ=255)

             aCAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCAccc                                                                       >  1:6291509/1‑93 (MQ=255)

              cAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCAcccc                                                                      <  1:5700389/93‑1 (MQ=255)

               aaCGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCAccccc                                                                     <  2:1563835/93‑2 (MQ=255)

                  gAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCtgt                                                                  >  2:8308317/1‑93 (MQ=255)

                  aaaaTGTTTAATGACTATAATAGCTTGCTTTAACCTTTTTTGATGATAAATGCGGGGTCGGAGGGGGC‑CCACCGGACACAGTTCACCCCCtgt                                                                  <  2:9045758/92‑1 (MQ=255)

                   aaaTGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCtgtg                                                                 >  2:4656830/1‑93 (MQ=255)

                   aaaTGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCtgtg                                                                 >  2:2551649/1‑93 (MQ=255)

                     aTGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGaa                                                               <  1:6596186/93‑1 (MQ=255)

                     aTGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGaa                                                               <  1:8239039/93‑1 (MQ=255)

                     aTGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGaa                                                               >  2:3185424/1‑93 (MQ=255)

                      tGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAg                                                              >  1:10031584/1‑93 (MQ=255)

                       ggTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAga                                                             >  1:6138015/1‑92 (MQ=255)

                       ggTTGATGACTATAATAGCCTGCGTAAAACGTTTTTGATGATAAATGCAGTGTCGGAGGCGGCGC‑ACCCGACACAGTTCACCCCCTGTGAAGt                                                             <  2:3984726/93‑1 (MQ=255)

                        gTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTa                                                            >  1:9338285/1‑93 (MQ=255)

                        cttGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTa                                                            <  1:1754949/92‑1 (MQ=255)

                         ttGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTaa                                                           >  2:6197378/1‑93 (MQ=255)

                         tggatgaCTATAAGAGCCTGCGTTAAACGTTTTTGATGAGAAATGCAGGGTCGGATGCGGCGC‑ACCGGACACAGTTCACCCCCTGTGAAGTaa                                                           <  2:954267/91‑1 (MQ=255)

                           gatgaCTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACt                                                         <  1:8146406/93‑1 (MQ=255)

                            atgaCTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTc                                                        <  2:1772460/93‑1 (MQ=255)

                              gaCTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCa                                                      >  1:3518590/1‑93 (MQ=255)

                              gaCTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCa                                                      <  2:1315101/93‑1 (MQ=255)

                               aCTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCAt                                                     <  1:1533038/93‑1 (MQ=255)

                               aCTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCAt                                                     <  1:2626913/93‑1 (MQ=255)

                                cTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATa                                                    >  1:45833/1‑93 (MQ=255)

                                 tataATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATaa                                                   >  1:6877925/1‑93 (MQ=255)

                                 tataATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATaa                                                   >  2:3410499/1‑93 (MQ=255)

                                  ataataGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATaaa                                                  >  2:8683259/1‑93 (MQ=255)

                                  ataataGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATaaa                                                  >  2:4780851/1‑93 (MQ=255)

                                   taataGCCTGCTTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ACCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAAt                                                 <  2:783798/93‑1 (MQ=255)

                                    aataGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATg                                                >  2:6445962/1‑93 (MQ=255)

                                     ataGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACGGTTCACCCCCTGTGAAGTAACTCCATAAATGc                                               <  2:981697/93‑1 (MQ=255)

                                     ataGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGc                                               >  1:8500668/1‑93 (MQ=255)

                                     ataGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGc                                               <  1:4292824/93‑1 (MQ=255)

                                      tggCCTGCGTTAACCTTTTTTGATGATAAATGCAGTGTCGTATGCGGCGC‑ATCCGCCACAGTTCACCCCCTGTGAAGTAACGCCATAAATGcc                                              <  1:2129703/91‑1 (MQ=255)

                                      taGCTTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGcc                                              <  2:7731994/93‑1 (MQ=255)

                                         ccTGCTTTAAACGTTTTTGATGATAAATGCATTGTCGGATGCGGC‑CCATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCa                                           <  1:2045006/93‑1 (MQ=255)

                                           tGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCata                                         <  1:8340996/93‑1 (MQ=255)

                                           tGCGTTAAACGTTTTTGATAATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCata                                         <  1:7910262/93‑1 (MQ=255)

                                            gCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCatat                                        >  2:6371589/1‑93 (MQ=255)

                                            gCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCatat                                        >  1:9645873/1‑93 (MQ=255)

                                            gCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCatat                                        >  1:5522629/1‑93 (MQ=255)

                                             cGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATc                                       <  1:1075484/93‑1 (MQ=255)

                                             cGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATc                                       >  1:9254380/1‑93 (MQ=255)

                                              gTTAAACGTTTTTGATGATAAATGCAGTGTCTGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATcc                                      <  2:4417000/93‑1 (MQ=255)

                                               ttAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCt                                     >  2:5769299/1‑93 (MQ=255)

                                               ttAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCt                                     <  1:7020909/93‑1 (MQ=255)

                                                tAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTg                                    >  2:5140124/1‑93 (MQ=255)

                                                tAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTg                                    >  1:5044015/1‑93 (MQ=255)

                                                tAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTAACCCCCTGTGAAGAAACTACATAAATGCCTCATATCCTg                                    >  2:8224619/1‑93 (MQ=255)

                                                  aaCGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGtt                                  <  2:8556666/93‑1 (MQ=255)

                                                  aaCGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGtt                                  >  2:5015223/1‑93 (MQ=255)

                                                   aCGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTa                                 >  2:352310/1‑93 (MQ=255)

                                                     gTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAAc                               <  2:4328566/93‑1 (MQ=255)

                                                       ttttGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAAccc                             <  1:3617573/93‑1 (MQ=255)

                                                       ttttGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAAccc                             <  2:8464029/93‑1 (MQ=255)

                                                        tttGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCg                            >  1:9842485/1‑93 (MQ=255)

                                                         ttGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCgg                           >  2:9268417/1‑93 (MQ=255)

                                                          tgatgaTAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGc                          <  1:4895261/93‑1 (MQ=255)

                                                          tgatgaTAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGc                          >  1:8746402/1‑93 (MQ=255)

                                                          tgatgaTAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGc                          >  1:8934513/1‑93 (MQ=255)

                                                            atgatAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaa                        >  2:2326325/1‑93 (MQ=255)

                                                             tgatAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaat                       >  2:4205885/1‑93 (MQ=255)

                                                              gatAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaata                      >  2:5890371/1‑93 (MQ=255)

                                                              gatAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaata                      >  2:121034/1‑93 (MQ=255)

                                                               atAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaataa                     >  1:2889900/1‑93 (MQ=255)

                                                               atAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaataa                     >  2:2223195/1‑93 (MQ=255)

                                                                tAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaataat                    >  2:6841759/1‑93 (MQ=255)

                                                                   aTGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAAtctc                 <  2:6048234/93‑1 (MQ=255)

                                                                    tGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTCACCCGGCAATAATCTcc                >  2:4498643/1‑93 (MQ=255)

                                                                    tGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTcc                >  2:555625/1‑93 (MQ=255)

                                                                    tGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTcc                >  2:6612993/1‑93 (MQ=255)

                                                                     gCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTccc               >  2:7502305/1‑92 (MQ=255)

                                                                     gCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTacg               >  2:3672148/1‑93 (MQ=255)

                                                                      cAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTccgc              >  1:4059758/1‑93 (MQ=255)

                                                                       aGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTccgcc             >  2:2993524/1‑93 (MQ=255)

                                                                            cGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTACGCCGGATg        >  2:6725551/1‑93 (MQ=255)

                                                                             ggATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGc       <  1:3301181/93‑1 (MQ=255)

                                                                             ggATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGc       <  1:3242958/93‑1 (MQ=255)

                                                                              gATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCt      >  1:4228604/1‑93 (MQ=255)

                                                                              gATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCt      >  2:3885917/1‑93 (MQ=255)

                                                                               aggcggcgC‑ACCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTg     <  2:7235057/91‑1 (MQ=255)

                                                                               aTGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTg     >  1:3186555/1‑93 (MQ=255)

                                                                                tGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGa    <  1:3997158/93‑1 (MQ=255)

                                                                                tGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGa    <  1:4448023/93‑1 (MQ=255)

                                                                                 gcggcgC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGac   >  1:222330/1‑93 (MQ=255)

GCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCGATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACA  >  NC_000913/4085587‑4085762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 286 total aligned reads displayed.

CGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCGATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATAT  >  NC_000913/4085582‑4085768

CGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCA                                                                                               >  SRR4302540.4672412/1‑94 (MQ=60)

   GAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCC                                                                                           <  SRR4302540.5517423/94‑1 (MQ=60)

    AGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCG                                                                                          >  SRR4302540.7578376/1‑94 (MQ=60)

     GCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGA                                                                                         >  SRR4302540.2858220/1‑94 (MQ=60)

     GCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGA                                                                                         >  SRR4302540.2858219/1‑94 (MQ=60)

      CATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGAC                                                                                        >  SRR4302540.657019/1‑94 (MQ=60)

      CATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGAC                                                                                        <  SRR4302540.918256/94‑1 (MQ=60)

      CATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGAC                                                                                        <  SRR4302540.2183972/94‑1 (MQ=60)

       ATTACGCGAGTACAACGAAATGGTGGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACA                                                                                       <  SRR4302540.4284707/94‑1 (MQ=60)

        TTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACAC                                                                                      <  SRR4302540.646500/94‑1 (MQ=60)

        TTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACAC                                                                                      >  SRR4302540.7746583/1‑94 (MQ=60)

         TACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACA                                                                                     >  SRR4302540.8877751/1‑94 (MQ=60)

          ACGGGAGTACAACAAAATGGTTGATGACTATAATAGCCTGCTTTAAACGTTTTTGATAATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAG                                                                                    <  SRR4302540.7677747/94‑1 (MQ=60)

          ACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAG                                                                                    <  SRR4302540.6489306/94‑1 (MQ=60)

          ACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAG                                                                                    <  SRR4302540.4767200/94‑1 (MQ=60)

          ACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAG                                                                                    <  SRR4302540.5733302/94‑1 (MQ=60)

           CGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGT                                                                                   >  SRR4302540.2097591/1‑94 (MQ=60)

           CGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGT                                                                                   <  SRR4302540.8732590/94‑1 (MQ=60)

           CGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGT                                                                                   >  SRR4302540.7469784/1‑94 (MQ=60)

            GCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTT                                                                                  <  SRR4302540.8264918/94‑1 (MQ=60)

            GCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTT                                                                                  >  SRR4302540.2346363/1‑94 (MQ=60)

             CGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTC                                                                                 >  SRR4302540.2904563/1‑94 (MQ=60)

             CGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTC                                                                                 >  SRR4302540.2476404/1‑94 (MQ=60)

              GAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCA                                                                                >  SRR4302540.8449767/1‑94 (MQ=60)

              GAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCA                                                                                >  SRR4302540.2583525/1‑94 (MQ=60)

              GAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCA                                                                                >  SRR4302540.1129130/1‑94 (MQ=60)

              GAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCA                                                                                >  SRR4302540.1129129/1‑94 (MQ=60)

               AGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCAC                                                                               <  SRR4302540.6768462/94‑1 (MQ=60)

               AGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCAC                                                                               <  SRR4302540.215822/94‑1 (MQ=60)

                GTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGATTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACC                                                                              >  SRR4302540.332027/1‑94 (MQ=60)

                 TACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCC                                                                             <  SRR4302540.1074171/94‑1 (MQ=60)

                 TACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCC                                                                             <  SRR4302540.1074168/94‑1 (MQ=60)

                 TACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCC                                                                             >  SRR4302540.6361550/1‑94 (MQ=60)

                 TACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCC                                                                             <  SRR4302540.9449330/94‑1 (MQ=60)

                  ACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCC                                                                            >  SRR4302540.887394/1‑94 (MQ=60)

                    AACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCT                                                                          <  SRR4302540.3515307/94‑1 (MQ=60)

                    AACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCT                                                                          >  SRR4302540.1779961/1‑94 (MQ=60)

                      CGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGT                                                                        <  SRR4302540.9532002/94‑1 (MQ=60)

                      CGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGT                                                                        <  SRR4302540.9532001/94‑1 (MQ=60)

                      CGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGT                                                                        <  SRR4302540.7884818/94‑1 (MQ=60)

                       GAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTG                                                                       <  SRR4302540.1044771/94‑1 (MQ=60)

                       GAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTG                                                                       >  SRR4302540.8835436/1‑94 (MQ=60)

                       GAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTG                                                                       >  SRR4302540.4744815/1‑94 (MQ=60)

                        AAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGA                                                                      >  SRR4302540.8419511/1‑94 (MQ=60)

                          ATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAG                                                                    <  SRR4302540.8334793/94‑1 (MQ=60)

                          ATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAG                                                                    >  SRR4302540.10148274/1‑94 (MQ=60)

                          ATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAG                                                                    >  SRR4302540.7688528/1‑94 (MQ=60)

                           TGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGT                                                                   <  SRR4302540.5673998/94‑1 (MQ=60)

                           TGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGA                                                                   >  SRR4302540.6206377/1‑94 (MQ=60)

                            GGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTA                                                                  >  SRR4302540.3948908/1‑94 (MQ=60)

                            GGTTGAGTACTAAAATAGCCGGGGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGC‑CCATCCGACACAGTTCACCCCCTGTGAAGTA                                                                  <  SRR4302540.7604115/94‑1 (MQ=60)

                             CTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAA                                                                 <  SRR4302540.1773965/94‑1 (MQ=60)

                              TTGATGAATATAATAGCCTGCGTTAACCGTTTTTGATGATAAAGGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAAC                                                                <  SRR4302540.10072668/94‑1 (MQ=60)

                               TGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACT                                                               <  SRR4302540.3112117/94‑1 (MQ=60)

                                GATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTC                                                              >  SRR4302540.109041/1‑94 (MQ=60)

                                GATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTC                                                              <  SRR4302540.8240979/94‑1 (MQ=60)

                                 ATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCC                                                             >  SRR4302540.6301088/1‑94 (MQ=60)

                                 ATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCC                                                             >  SRR4302540.3094897/1‑94 (MQ=60)

                                   GACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCAT                                                           >  SRR4302540.4401786/1‑94 (MQ=60)

                                   GACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCAT                                                           >  SRR4302540.4063461/1‑94 (MQ=60)

                                   GACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCAT                                                           >  SRR4302540.3654622/1‑94 (MQ=60)

                                   GACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACACCCTGTGAAGTAACTCCAT                                                           >  SRR4302540.2387900/1‑94 (MQ=60)

                                    ACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTCACTCCATA                                                          >  SRR4302540.3297432/1‑94 (MQ=60)

                                    ACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATA                                                          <  SRR4302540.1550008/94‑1 (MQ=60)

                                    ACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATA                                                          >  SRR4302540.5619845/1‑94 (MQ=60)

                                    ACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATA                                                          <  SRR4302540.2655328/94‑1 (MQ=60)

                                    ACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATA                                                          >  SRR4302540.46300/1‑94 (MQ=60)

                                     CTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACTCCCTGTGAAGGAACTCCATAA                                                         >  SRR4302540.8522000/1‑94 (MQ=60)

                                     CTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAA                                                         <  SRR4302540.1117808/94‑1 (MQ=60)

                                     CTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAA                                                         >  SRR4302540.6954161/1‑94 (MQ=60)

                                     CTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAA                                                         >  SRR4302540.3753646/1‑94 (MQ=60)

                                     CTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAA                                                         >  SRR4302540.8295668/1‑94 (MQ=60)

                                      TATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGCACCAGTTCACCCCCTGTGAAGTAACTCAATAAA                                                        >  SRR4302540.7698353/1‑94 (MQ=60)

                                      TATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACGGTTCACCCCCTGTGAAGTAACTCCATAAA                                                        >  SRR4302540.8977698/1‑94 (MQ=60)

                                      TATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAA                                                        >  SRR4302540.3414043/1‑94 (MQ=60)

                                      TATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAA                                                        >  SRR4302540.6870224/1‑94 (MQ=60)

                                      TATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAA                                                        <  SRR4302540.8171444/94‑1 (MQ=60)

                                      TATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAA                                                        >  SRR4302540.4173394/1‑94 (MQ=60)

                                      TATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAA                                                        >  SRR4302540.4166873/1‑94 (MQ=60)

                                       ATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAAT                                                       >  SRR4302540.3472269/1‑94 (MQ=60)

                                       ATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAAT                                                       <  SRR4302540.6051830/94‑1 (MQ=60)

                                       ATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAAT                                                       <  SRR4302540.3621834/94‑1 (MQ=60)

                                       ATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGATCACCCCCTGTGAAGTAACTCCATAAAT                                                       >  SRR4302540.3238488/1‑94 (MQ=60)

                                         AATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGC                                                     >  SRR4302540.8599443/1‑94 (MQ=60)

                                         AATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGC                                                     >  SRR4302540.4529472/1‑94 (MQ=60)

                                          ATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCC                                                    <  SRR4302540.4339526/94‑1 (MQ=60)

                                          ATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCC                                                    >  SRR4302540.7711886/1‑94 (MQ=60)

                                          ATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCC                                                    >  SRR4302540.7348238/1‑94 (MQ=60)

                                          ATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCC                                                    <  SRR4302540.9668509/94‑1 (MQ=60)

                                          ATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCC                                                    >  SRR4302540.4702745/1‑94 (MQ=60)

                                          ATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGGAACTCCATACATGAC                                                    >  SRR4302540.1194013/1‑94 (MQ=60)

                                           TAGCCTGCGTCAAACGTTTTTGATGATAAATGCTGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCT                                                   <  SRR4302540.4090184/94‑1 (MQ=60)

                                            AGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTC                                                  >  SRR4302540.4284318/1‑94 (MQ=60)

                                            AGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTC                                                  >  SRR4302540.5381101/1‑94 (MQ=60)

                                            AGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTC                                                  >  SRR4302540.10099724/1‑94 (MQ=60)

                                             GCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCA                                                 >  SRR4302540.876020/1‑94 (MQ=60)

                                              tctgctgacccgctcttccgatctagcgctttTGTCGGATGCTGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCAT                                                <  SRR4302540.6460642/62‑1 (MQ=60)

                                              CCTGCTTTAAACGTTTTTGATGATAAATGCATTGTCGGATGCGGC‑CCATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCAT                                                <  SRR4302540.2067035/94‑1 (MQ=60)

                                              CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCAT                                                <  SRR4302540.4605289/94‑1 (MQ=60)

                                              CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCAT                                                <  SRR4302540.6508576/94‑1 (MQ=60)

                                              CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACGACATAAATGCCTCAT                                                >  SRR4302540.6949950/1‑94 (MQ=60)

                                              CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGAAACAGGTCACCCCCTGTGAAGTCACTACATAAATGCCTCCT                                                >  SRR4302540.3246447/1‑94 (MQ=60)

                                               CTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATA                                               >  SRR4302540.2594016/1‑94 (MQ=60)

                                               CTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATA                                               >  SRR4302540.2150947/1‑94 (MQ=60)

                                               CTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATA                                               >  SRR4302540.2798235/1‑94 (MQ=60)

                                               CTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATA                                               >  SRR4302540.434865/1‑94 (MQ=60)

                                                TGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATAT                                              >  SRR4302540.5583441/1‑94 (MQ=60)

                                                TGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATAT                                              <  SRR4302540.8437811/94‑1 (MQ=60)

                                                TGCGTTAAACGTTTTTGATAATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATAT                                              <  SRR4302540.8002497/94‑1 (MQ=60)

                                                 GCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATC                                             <  SRR4302540.1881561/94‑1 (MQ=60)

                                                 GCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATC                                             >  SRR4302540.9365133/1‑94 (MQ=60)

                                                 GCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATC                                             <  SRR4302540.854414/94‑1 (MQ=60)

                                                  ggttaaactgTTTTGATGATAGATGCAGGGTCGGATGCGGC‑CCATCCGACACAGTTCGCCCCCTGTGAAGTAACTCCATAAATGCCTCATATCC                                            <  SRR4302540.1086476/84‑1 (MQ=60)

                                                  acTTAAAAGTTTTTGATGAAAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCC                                            <  SRR4302540.7038645/92‑1 (MQ=60)

                                                  CGTTAAACGTTTTTGATGATATTTGCATTGTCGGATGCGGCGC‑ATCCGACTTAGTTCACCCCCTTTGTAGTAACTCCATAAACGTTTCATATCC                                            >  SRR4302540.9058846/1‑94 (MQ=24)

                                                   GTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCT                                           <  SRR4302540.4868121/94‑1 (MQ=60)

                                                   GTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCT                                           >  SRR4302540.6397387/1‑94 (MQ=60)

                                                    TTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTG                                          <  SRR4302540.6976872/94‑1 (MQ=60)

                                                    TTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTG                                          >  SRR4302540.6805734/1‑94 (MQ=60)

                                                    TTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTG                                          >  SRR4302540.10017752/1‑94 (MQ=60)

                                                    TTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTG                                          >  SRR4302540.5098574/1‑94 (MQ=60)

                                                     TAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGT                                         >  SRR4302540.9952505/1‑94 (MQ=60)

                                                     TAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGT                                         >  SRR4302540.4775963/1‑94 (MQ=60)

                                                     TAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCAACCCCTGGGAAGTAACTCCCTAAATGCCTGATATCCTGT                                         >  SRR4302540.1970145/1‑94 (MQ=60)

                                                      AAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTT                                        >  SRR4302540.922788/1‑94 (MQ=60)

                                                      AAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTT                                        <  SRR4302540.4365818/94‑1 (MQ=60)

                                                         CGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAAC                                     >  SRR4302540.5619135/1‑94 (MQ=60)

                                                         CGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAAC                                     <  SRR4302540.7673533/94‑1 (MQ=60)

                                                         CGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAAC                                     <  SRR4302540.2128487/94‑1 (MQ=60)

                                                         CGTTTTGGATGATAAATGCGGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGGGAAGTAACTCCATAAATGCCTCATATCCTGTTAAC                                     <  SRR4302540.4521189/94‑1 (MQ=60)

                                                           TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCC                                   >  SRR4302540.7115082/1‑94 (MQ=60)

                                                           TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCC                                   <  SRR4302540.985605/94‑1 (MQ=60)

                                                            TTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCG                                  >  SRR4302540.9957545/1‑94 (MQ=60)

                                                            TTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCG                                  <  SRR4302540.8317137/94‑1 (MQ=60)

                                                            TTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCG                                  <  SRR4302540.3657144/94‑1 (MQ=60)

                                                              TTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGC                                >  SRR4302540.9038996/1‑94 (MQ=60)

                                                               TGATGATAAATGCAGTGTCGGATGCGGCTC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCA                               <  SRR4302540.4947912/94‑1 (MQ=60)

                                                               TGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCA                               <  SRR4302540.1720410/94‑1 (MQ=60)

                                                               TGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCA                               <  SRR4302540.4947911/94‑1 (MQ=60)

                                                                GATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAA                              >  SRR4302540.7359202/1‑94 (MQ=60)

                                                                GATGATAAATGCAGTGTCGGATGCGGC‑CCATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAA                              <  SRR4302540.3732544/94‑1 (MQ=60)

                                                                 ATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAAT                             >  SRR4302540.6740937/1‑94 (MQ=60)

                                                                 ATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAAT                             >  SRR4302540.3563224/1‑94 (MQ=60)

                                                                 ATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAAT                             <  SRR4302540.422681/94‑1 (MQ=60)

                                                                 ATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAAT                             >  SRR4302540.8004508/1‑94 (MQ=60)

                                                                 ATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAAT                             >  SRR4302540.7955264/1‑94 (MQ=60)

                                                                 ATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAAT                             <  SRR4302540.6220912/94‑1 (MQ=60)

                                                                  TGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATA                            >  SRR4302540.1954529/1‑94 (MQ=60)

                                                                   GATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAA                           >  SRR4302540.2921385/1‑94 (MQ=60)

                                                                   GATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAA                           >  SRR4302540.3374832/1‑94 (MQ=60)

                                                                   GATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACACCATAAACGCCTCATATCCTGTTAACCCGGCAATAA                           <  SRR4302540.7006895/94‑1 (MQ=60)

                                                                    ATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAAT                          <  SRR4302540.3611308/94‑1 (MQ=60)

                                                                     TAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATC                         <  SRR4302540.2845871/94‑1 (MQ=60)

                                                                     TAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATC                         >  SRR4302540.3273305/1‑94 (MQ=60)

                                                                       AATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTC                       <  SRR4302540.5961977/94‑1 (MQ=60)

                                                                        ATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCC                      >  SRR4302540.1150603/1‑94 (MQ=60)

                                                                        ATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCC                      <  SRR4302540.499437/94‑1 (MQ=60)

                                                                        ATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCC                      <  SRR4302540.8402276/94‑1 (MQ=60)

                                                                        ATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTACATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCC                      >  SRR4302540.2397433/1‑94 (MQ=60)

                                                                         TGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCG                     >  SRR4302540.6694522/1‑94 (MQ=60)

                                                                          GCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCCCCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGC                    <  SRR4302540.5199221/94‑1 (MQ=60)

                                                                          GCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGC                    >  SRR4302540.4284218/1‑94 (MQ=60)

                                                                           CAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCC                   >  SRR4302540.9395035/1‑94 (MQ=60)

                                                                           CAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCC                   <  SRR4302540.2469998/94‑1 (MQ=60)

                                                                           CAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCC                   <  SRR4302540.6695687/94‑1 (MQ=60)

                                                                            AGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCG                  <  SRR4302540.4099652/94‑1 (MQ=60)

                                                                            AGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCG                  <  SRR4302540.2169262/94‑1 (MQ=60)

                                                                            AGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCG                  >  SRR4302540.4827547/1‑94 (MQ=60)

                                                                             GTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGG                 >  SRR4302540.6376786/1‑94 (MQ=60)

                                                                             GTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGG                 >  SRR4302540.9575024/1‑94 (MQ=60)

                                                                             GTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGG                 >  SRR4302540.9422996/1‑94 (MQ=60)

                                                                              TGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGA                <  SRR4302540.6623902/94‑1 (MQ=60)

                                                                               GTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGAT               <  SRR4302540.1671227/94‑1 (MQ=60)

                                                                               GTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAACCCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGAT               <  SRR4302540.6756181/94‑1 (MQ=60)

                                                                                 CGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGC             >  SRR4302540.4330928/1‑94 (MQ=60)

                                                                                  GGATGCGGCGC‑ATCCGCCACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCT            <  SRR4302540.2952987/94‑1 (MQ=60)

                                                                                  GGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCT            >  SRR4302540.9926507/1‑94 (MQ=60)

                                                                                  GGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCT            >  SRR4302540.4274638/1‑94 (MQ=60)

                                                                                  GGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCT            >  SRR4302540.5842358/1‑94 (MQ=60)

                                                                                  GGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCT            >  SRR4302540.2900032/1‑94 (MQ=60)

                                                                                  GGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCT            >  SRR4302540.3085942/1‑94 (MQ=60)

                                                                                  GGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCT            <  SRR4302540.3278258/94‑1 (MQ=60)

                                                                                  GGAGGCGGCGC‑AGCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCT            <  SRR4302540.7157598/94‑1 (MQ=60)

                                                                                    ATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGA          >  SRR4302540.8156665/1‑94 (MQ=60)

                                                                                    ATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGA          <  SRR4302540.3328357/94‑1 (MQ=60)

                                                                                     TGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGAC         >  SRR4302540.224481/1‑94 (MQ=60)

                                                                                     TGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGAC         <  SRR4302540.1587866/94‑1 (MQ=60)

                                                                                     TGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGAC         <  SRR4302540.4040181/94‑1 (MQ=60)

                                                                                        GGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACA      <  SRR4302540.9323902/94‑1 (MQ=60)

                                                                                        GGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACA      >  SRR4302540.9558247/1‑94 (MQ=60)

                                                                                          CGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAAT    >  SRR4302540.8115986/1‑94 (MQ=60)

                                                                                             CATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATAT  <  SRR4302540.5779857/94‑1 (MQ=60)

                                                                                             CATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATAT  <  SRR4302540.1414337/94‑1 (MQ=60)

                                                                                             CATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATAT  <  SRR4302540.9847232/94‑1 (MQ=60)

                                                                                             CATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATAT  <  SRR4302540.9759784/94‑1 (MQ=60)

                                                                                             CATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATAT  >  SRR4302540.4974240/1‑94 (MQ=60)

                                                                                             CATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATAT  >  SRR4302540.223751/1‑94 (MQ=60)

                                                                                             CATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATAT  <  SRR4302540.8493592/94‑1 (MQ=60)

                                                                                             CATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATAT  <  SRR4302540.7928389/94‑1 (MQ=60)

                                                                                             CATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCAGATGCTGACACAATAT  >  SRR4302540.4974238/1‑94 (MQ=60)

CGAGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCGATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATAT  >  NC_000913/4085582‑4085768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`