Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,085,675 Δ1 bp intergenic (+38/+13) yiiG → / ← frvR hypothetical protein/putative frv operon regulatory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,085,6750G.100.0% 1070.3 / NA 233intergenic (+38/+13)yiiG/frvRhypothetical protein/putative frv operon regulatory protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (143/90);  total (143/90)

Only 100 of 247 total aligned reads displayed.

CATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCGATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACA  >  NC_000913/4085588‑4085762
                                                                                       |                                                                                       
   tACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAGTGCAGTGTCGGATGCGGCGC‑ATCCGacac                                                                                <  1:2018759/93‑1 (MQ=255)
    aCGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGAcaca                                                                               <  1:669172/93‑1 (MQ=255)
    aCGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGAcaca                                                                               <  2:3915206/93‑1 (MQ=255)
      gcgAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGt                                                                             >  2:5553339/1‑93 (MQ=255)
       cgAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGtt                                                                            >  2:2783465/1‑93 (MQ=255)
        gAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTc                                                                           >  2:4886435/1‑93 (MQ=255)
          gTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCAc                                                                         >  2:2957061/1‑93 (MQ=255)
             cAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCAcccc                                                                      >  1:6182619/1‑93 (MQ=255)
             cAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCCGCGC‑ATCCGACACAGTAGAcccc                                                                      >  2:4839604/1‑93 (MQ=255)
              aaCGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCAccccc                                                                     >  2:5808884/1‑92 (MQ=255)
               aCGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCAccccct                                                                    >  1:2972927/1‑91 (MQ=255)
                  aaaTGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCtgtg                                                                 >  1:2608808/1‑93 (MQ=255)
                    aTGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGaa                                                               <  1:4749744/93‑1 (MQ=255)
                    aTGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGaa                                                               <  1:2850135/93‑1 (MQ=255)
                     tGGTTGATGACTATAATAGCCTGCGTTAAACTTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAg                                                              >  1:3629898/1‑93 (MQ=255)
                     tGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAg                                                              >  2:1840989/1‑93 (MQ=255)
                      ggTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGt                                                             <  1:962590/93‑1 (MQ=255)
                      ggTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGt                                                             >  2:712616/1‑93 (MQ=255)
                          gatgaCTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACt                                                         >  1:228671/1‑93 (MQ=255)
                          gatgaCTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACt                                                         >  2:4986521/1‑93 (MQ=255)
                          gatgaCTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACt                                                         >  1:3759361/1‑93 (MQ=255)
                              attataATAGCCTGCTTTAACCGTTTTTGATAAAAAATGCAGTGTCGGATGCGGCGC‑ACCCGACACAGTTCACCCCCTGTGAAGTAACTCCAt                                                     <  2:2214/91‑1 (MQ=255)
                              aCTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCAt                                                     >  2:2315354/1‑93 (MQ=255)
                              aCTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCAt                                                     >  1:6033210/1‑93 (MQ=255)
                              aCTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCAt                                                     <  1:2397729/93‑1 (MQ=255)
                               cTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTATCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATa                                                    <  1:1967225/93‑1 (MQ=255)
                                tataATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATaa                                                   >  2:2026698/1‑93 (MQ=255)
                                 ataataGCGTGCGTTAAACTTTTTGGATGATAAATGCAGTGTCGGAGGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATaaa                                                  <  2:3345016/93‑1 (MQ=255)
                                 ataataGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATaaa                                                  <  1:3803985/93‑1 (MQ=255)
                                 ataataGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATaaa                                                  <  2:138217/93‑1 (MQ=255)
                                  taataGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAAt                                                 >  1:4775905/1‑93 (MQ=255)
                                   aataGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATg                                                <  1:5097905/93‑1 (MQ=255)
                                   aataGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATg                                                >  2:5220116/1‑93 (MQ=255)
                                   aataGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATg                                                >  2:1276007/1‑93 (MQ=255)
                                   aataGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATg                                                >  1:5062222/1‑93 (MQ=255)
                                     taGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGGAACTCCATAAATGcc                                              >  2:369171/1‑93 (MQ=255)
                                        cgtGCGTCAACCTTTTTTGATGATAAGTGCTGGGTGGGATGGGGCAC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCa                                           <  1:1089285/91‑1 (MQ=255)
                                        ccTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCa                                           >  1:35925/1‑93 (MQ=255)
                                         cTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCat                                          >  2:314796/1‑93 (MQ=255)
                                         cTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACACCCTGTGAAGTAACTCCATAAATGCCTCat                                          >  2:6185806/1‑93 (MQ=255)
                                         cTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCAACCCCTGTGAAGTAACTCCATAAATGCCTCat                                          >  2:1056045/1‑93 (MQ=255)
                                          tGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCata                                         >  2:3826029/1‑93 (MQ=255)
                                           gCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCatat                                        >  2:891385/1‑93 (MQ=255)
                                            cGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATc                                       <  1:2659113/93‑1 (MQ=255)
                                            cGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATc                                       <  2:4782320/93‑1 (MQ=255)
                                              ttAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCt                                     <  1:187117/93‑1 (MQ=255)
                                               tAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTg                                    >  1:353160/1‑93 (MQ=255)
                                               tAAACGTTTTTGATGATAAATCCAGTGTCGGATGCGGC‑CCACCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTg                                    <  1:4302518/93‑1 (MQ=255)
                                                aaaCGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGt                                   >  2:2282779/1‑93 (MQ=255)
                                                aaaCGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGt                                   <  2:1983849/93‑1 (MQ=255)
                                                aaaCGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGt                                   >  2:1387785/1‑93 (MQ=255)
                                                aaaCGTTTTTGATGATAAATGCAGCGTCGGATGCGGCGCG‑TCCGACACAGTTCACCCCCTGTGAAGCAACTCCATAAATACCTCATATCCTGt                                   >  2:3010936/1‑93 (MQ=255)
                                                 aaCGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGtt                                  >  1:5282092/1‑93 (MQ=255)
                                                 aaCGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGtt                                  >  2:4245748/1‑93 (MQ=255)
                                                 aaCGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGtt                                  >  1:4787607/1‑93 (MQ=255)
                                                  aCGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTa                                 >  1:4062939/1‑93 (MQ=255)
                                                   cGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTaa                                <  1:454271/93‑1 (MQ=255)
                                                   cGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTaa                                <  2:1590583/93‑1 (MQ=255)
                                                    gTTTTTGATGATTAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGCAGTAACTCCATAAATGCCTCATATCCTGTTAAc                               >  1:3408924/1‑93 (MQ=255)
                                                    gTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAAc                               >  2:4311031/1‑93 (MQ=255)
                                                     tttttGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAAcc                              >  2:1648014/1‑93 (MQ=255)
                                                      ttttGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAAccc                             >  1:1883150/1‑93 (MQ=255)
                                                        ttGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCgg                           >  1:1598059/1‑93 (MQ=255)
                                                         tgatgaTAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGc                          <  2:2343433/93‑1 (MQ=255)
                                                          gatgatAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCa                         >  2:3462158/1‑93 (MQ=255)
                                                           atgatAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaa                        >  1:831896/1‑93 (MQ=255)
                                                             gatAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaata                      <  1:417498/93‑1 (MQ=255)
                                                             gatAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaata                      <  1:5453664/93‑1 (MQ=255)
                                                             gatAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaata                      <  1:1840978/93‑1 (MQ=255)
                                                             gatAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaata                      <  2:3610379/93‑1 (MQ=255)
                                                              atAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGGTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaataa                     >  2:5997634/1‑93 (MQ=255)
                                                               tAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCaataat                    >  2:5108372/1‑93 (MQ=255)
                                                                aaaTGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAAtc                   >  1:3044038/1‑93 (MQ=255)
                                                                  aTGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAAtctc                 <  1:1180015/93‑1 (MQ=255)
                                                                  aTGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAAtctc                 <  1:5826463/93‑1 (MQ=255)
                                                                   tGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTcc                >  2:3373042/1‑93 (MQ=255)
                                                                   tGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTcc                >  2:4519575/1‑93 (MQ=255)
                                                                   tGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTcc                >  1:2843392/1‑93 (MQ=255)
                                                                   tGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTcc                >  1:1697897/1‑93 (MQ=255)
                                                                    gCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTccg               <  1:5631597/93‑1 (MQ=255)
                                                                    gCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTccg               >  2:608225/1‑93 (MQ=255)
                                                                     cAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTccgc              <  2:457223/93‑1 (MQ=255)
                                                                     cAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTccgc              <  2:4699669/93‑1 (MQ=255)
                                                                      aGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTccgcc             >  1:132467/1‑93 (MQ=255)
                                                                      aGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTccgcc             >  2:590693/1‑93 (MQ=255)
                                                                      aGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTccgcc             <  2:5277289/93‑1 (MQ=255)
                                                                       gtgtCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTccgccg            >  1:638766/1‑93 (MQ=255)
                                                                       gtgtCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTccgccg            >  1:2229845/1‑93 (MQ=255)
                                                                       gtgtCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACACGGCAATAATCTccgccg            >  2:247988/1‑93 (MQ=255)
                                                                        tgtCGGATGCGGCGC‑ATCCGAAACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCgg           >  1:1621020/1‑93 (MQ=255)
                                                                         ggcGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGa          <  1:4697761/91‑1 (MQ=255)
                                                                          tCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGAt         <  2:5687153/93‑1 (MQ=255)
                                                                          tCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGAt         <  1:3249069/93‑1 (MQ=255)
                                                                           cGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATg        <  1:5148091/93‑1 (MQ=255)
                                                                             gATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCt      <  2:1927780/93‑1 (MQ=255)
                                                                              aTGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTg     >  1:3324494/1‑93 (MQ=255)
                                                                               tGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGa    <  2:4467371/93‑1 (MQ=255)
                                                                               tGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGa    <  2:786375/93‑1 (MQ=255)
                                                                               tGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGa    <  2:4023850/93‑1 (MQ=255)
                                                                                 cggcgC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGaca  <  1:952608/93‑1 (MQ=255)
                                                                                       |                                                                                       
CATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCGATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACA  >  NC_000913/4085588‑4085762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCGATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATAT  >  NC_000913/4085584‑4085768
                                                                                           |                                                                                             
AGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATC                                                                                            >  SRR4302533.4411104/1‑94 (MQ=60)
 GAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCC                                                                                           <  SRR4302533.4684255/94‑1 (MQ=60)
 GAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCC                                                                                           <  SRR4302533.5161544/94‑1 (MQ=60)
    CATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGAC                                                                                        <  SRR4302533.4736335/94‑1 (MQ=60)
      TTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACAC                                                                                      >  SRR4302533.736517/1‑94 (MQ=60)
      TTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACAC                                                                                      >  SRR4302533.6250440/1‑94 (MQ=60)
       TACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAGTGCAGTGTCGGATGCGGCGC‑ATCCGACACA                                                                                     <  SRR4302533.2031195/94‑1 (MQ=60)
        ACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAG                                                                                    <  SRR4302533.1240381/94‑1 (MQ=60)
        ACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAG                                                                                    <  SRR4302533.673124/94‑1 (MQ=60)
        ACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAG                                                                                    >  SRR4302533.3174649/1‑94 (MQ=60)
         CGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGT                                                                                   <  SRR4302533.35803/94‑1 (MQ=60)
          GCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTT                                                                                  >  SRR4302533.3621869/1‑94 (MQ=60)
          GCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTT                                                                                  <  SRR4302533.2030672/94‑1 (MQ=60)
          GCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTT                                                                                  >  SRR4302533.2663145/1‑94 (MQ=60)
               TACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCC                                                                             >  SRR4302533.1432595/1‑94 (MQ=60)
                ACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCC                                                                            >  SRR4302533.6219744/1‑94 (MQ=60)
                 CAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCC                                                                           >  SRR4302533.5249300/1‑94 (MQ=60)
                  AACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCT                                                                          >  SRR4302533.2991118/1‑94 (MQ=60)
                     GAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTG                                                                       >  SRR4302533.2624827/1‑94 (MQ=60)
                     GAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTG                                                                       >  SRR4302533.4812451/1‑94 (MQ=60)
                      AAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGA                                                                      <  SRR4302533.5215342/94‑1 (MQ=60)
                      AAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGA                                                                      >  SRR4302533.2170576/1‑94 (MQ=60)
                       AATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAA                                                                     <  SRR4302533.2789008/94‑1 (MQ=60)
                        ATGGTTGATGACTATAATAGCCTGCGTTAAACTTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAG                                                                    >  SRR4302533.3652232/1‑94 (MQ=60)
                        ATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAG                                                                    <  SRR4302533.3056765/94‑1 (MQ=60)
                        ATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAG                                                                    <  SRR4302533.4778542/94‑1 (MQ=60)
                        ATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAG                                                                    <  SRR4302533.2867670/94‑1 (MQ=60)
                          GGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTA                                                                  <  SRR4302533.968247/94‑1 (MQ=60)
                            TTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAAC                                                                <  SRR4302533.3784928/94‑1 (MQ=60)
                             TGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACT                                                               <  SRR4302533.3332128/94‑1 (MQ=60)
                             TGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACT                                                               >  SRR4302533.3782406/1‑94 (MQ=60)
                             TGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACT                                                               >  SRR4302533.230090/1‑94 (MQ=60)
                               ATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCC                                                             >  SRR4302533.3659895/1‑94 (MQ=60)
                               ATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCC                                                             >  SRR4302533.1620760/1‑94 (MQ=60)
                                TGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCA                                                            >  SRR4302533.6086218/1‑94 (MQ=60)
                                 GACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCAT                                                           >  SRR4302533.6069772/1‑94 (MQ=60)
                                 GACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCAT                                                           >  SRR4302533.4092725/1‑94 (MQ=60)
                                  ACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATA                                                          <  SRR4302533.2412530/94‑1 (MQ=60)
                                  ACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATA                                                          >  SRR4302533.1887081/1‑94 (MQ=60)
                                  ACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATA                                                          >  SRR4302533.4067135/1‑94 (MQ=60)
                                   CTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCAACCCCTGTGAAGTAACTCCATAA                                                         >  SRR4302533.966772/1‑94 (MQ=60)
                                   CTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTATCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAA                                                         <  SRR4302533.1979247/94‑1 (MQ=60)
                                     ATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAAT                                                       >  SRR4302533.4804839/1‑94 (MQ=60)
                                     ATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAAT                                                       <  SRR4302533.4618725/94‑1 (MQ=60)
                                     ATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAAT                                                       <  SRR4302533.3827263/94‑1 (MQ=60)
                                      TAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATG                                                      >  SRR4302533.4057694/1‑94 (MQ=60)
                                      TAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATG                                                      <  SRR4302533.964632/94‑1 (MQ=60)
                                      TAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATG                                                      >  SRR4302533.5092809/1‑94 (MQ=60)
                                       AATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGC                                                     <  SRR4302533.5128705/94‑1 (MQ=60)
                                        ATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCC                                                    >  SRR4302533.5412650/1‑94 (MQ=60)
                                         TACCCTGCTTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCT                                                   <  SRR4302533.65228/94‑1 (MQ=60)
                                           GCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCA                                                 >  SRR4302533.36164/1‑94 (MQ=60)
                                            CCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCAT                                                <  SRR4302533.4309331/94‑1 (MQ=60)
                                             CTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATA                                               >  SRR4302533.2389575/1‑94 (MQ=60)
                                             CTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATA                                               >  SRR4302533.342001/1‑94 (MQ=60)
                                             CTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATA                                               >  SRR4302533.3402210/1‑94 (MQ=60)
                                             CTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATA                                               >  SRR4302533.2884577/1‑94 (MQ=60)
                                             CTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATA                                               >  SRR4302533.1934840/1‑94 (MQ=60)
                                                CGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCC                                            <  SRR4302533.2675446/94‑1 (MQ=60)
                                                 GTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCT                                           >  SRR4302533.1854246/1‑94 (MQ=60)
                                                  TTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTG                                          >  SRR4302533.1651918/1‑94 (MQ=60)
                                                  TTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTG                                          >  SRR4302533.5100826/1‑94 (MQ=60)
                                                  TTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTG                                          <  SRR4302533.188280/94‑1 (MQ=60)
                                                  TTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTG                                          <  SRR4302533.4674852/94‑1 (MQ=60)
                                                  TTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTG                                          >  SRR4302533.355314/1‑94 (MQ=60)
                                                   TAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGT                                         >  SRR4302533.1873598/1‑94 (MQ=60)
                                                   TAAACGTTTTTGATGATAAATCCAGTGTCGGATGCGGC‑CCACCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGT                                         <  SRR4302533.4328402/94‑1 (MQ=60)
                                                    AAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTT                                        >  SRR4302533.2464332/1‑94 (MQ=60)
                                                    AAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTT                                        >  SRR4302533.5313739/1‑94 (MQ=60)
                                                    AAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTT                                        >  SRR4302533.4816603/1‑94 (MQ=60)
                                                     AACGTTTTTGATGATAAATGCAGTGTCGGATGCGGGGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATACTGTTA                                       >  SRR4302533.3904068/1‑94 (MQ=60)
                                                     AACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTA                                       >  SRR4302533.4087669/1‑94 (MQ=60)
                                                      ACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAA                                      >  SRR4302533.868889/1‑94 (MQ=60)
                                                      ACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAA                                      <  SRR4302533.225046/94‑1 (MQ=60)
                                                       CGTTTTTGATGATTAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGCAGTAACTCCATAAATGCCTCATATCCTGTTAAC                                     >  SRR4302533.3429982/1‑94 (MQ=60)
                                                       CGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAAC                                     <  SRR4302533.456986/94‑1 (MQ=60)
                                                       CGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAAC                                     >  SRR4302533.348804/1‑94 (MQ=60)
                                                        GTTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACC                                    >  SRR4302533.4805997/1‑94 (MQ=60)
                                                         TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCC                                   >  SRR4302533.1894649/1‑94 (MQ=60)
                                                         TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCC                                   <  SRR4302533.4207326/94‑1 (MQ=60)
                                                         TTTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCC                                   >  SRR4302533.2319720/1‑94 (MQ=60)
                                                          TTTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCG                                  <  SRR4302533.5591719/94‑1 (MQ=60)
                                                           TTTGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGG                                 >  SRR4302533.1607733/1‑94 (MQ=60)
                                                             TGATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCA                               >  SRR4302533.2664153/1‑94 (MQ=60)
                                                              GATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAA                              >  SRR4302533.836772/1‑94 (MQ=60)
                                                              GATGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGATCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAA                              >  SRR4302533.5597895/1‑94 (MQ=60)
                                                                TGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATA                            >  SRR4302533.1099626/1‑94 (MQ=60)
                                                                TGATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATA                            >  SRR4302533.2105062/1‑94 (MQ=60)
                                                                 GATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAA                           <  SRR4302533.5486514/94‑1 (MQ=60)
                                                                 GATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAA                           <  SRR4302533.1852206/94‑1 (MQ=60)
                                                                 GATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAA                           <  SRR4302533.420003/94‑1 (MQ=60)
                                                                 GATAAATGCAGTGTCGGATGCGACGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAA                           <  SRR4302533.5108979/94‑1 (MQ=60)
                                                                  ATAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAAT                          >  SRR4302533.5080443/1‑94 (MQ=60)
                                                                   TAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATC                         >  SRR4302533.3062672/1‑94 (MQ=60)
                                                                   TAAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATC                         >  SRR4302533.3605433/1‑94 (MQ=60)
                                                                    AAATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCT                        >  SRR4302533.5150116/1‑94 (MQ=60)
                                                                      ATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCC                      >  SRR4302533.1708182/1‑94 (MQ=60)
                                                                      ATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCC                      >  SRR4302533.2860890/1‑94 (MQ=60)
                                                                      ATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCC                      <  SRR4302533.1187103/94‑1 (MQ=60)
                                                                      ATGCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCC                      <  SRR4302533.5861670/94‑1 (MQ=60)
                                                                        GCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGC                    <  SRR4302533.5665654/94‑1 (MQ=60)
                                                                        GCAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGC                    >  SRR4302533.3767555/1‑94 (MQ=60)
                                                                         CAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCC                   <  SRR4302533.1765333/94‑1 (MQ=60)
                                                                         CAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCC                   >  SRR4302533.730485/1‑94 (MQ=60)
                                                                         CAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCC                   >  SRR4302533.133299/1‑94 (MQ=60)
                                                                         CAGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCC                   >  SRR4302533.3107453/1‑94 (MQ=60)
                                                                         CAGTGCGGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCC                   <  SRR4302533.571664/94‑1 (MQ=60)
                                                                          AGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCG                  >  SRR4302533.2243607/1‑94 (MQ=60)
                                                                          AGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCG                  <  SRR4302533.4784903/94‑1 (MQ=60)
                                                                          AGTGTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCG                  >  SRR4302533.642523/1‑94 (MQ=60)
                                                                           GTGTCGGATGCGGCGC‑ATCCGAAACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGG                 >  SRR4302533.1630841/1‑94 (MQ=60)
                                                                             GTGGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGAT               <  SRR4302533.4433971/94‑1 (MQ=60)
                                                                             GTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGAT               <  SRR4302533.5914877/94‑1 (MQ=60)
                                                                             GTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGAT               >  SRR4302533.5986500/1‑94 (MQ=60)
                                                                             GTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGAT               >  SRR4302533.6281984/1‑94 (MQ=60)
                                                                             GTCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGAT               >  SRR4302533.1482381/1‑94 (MQ=60)
                                                                             GGCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGAT               <  SRR4302533.4726238/94‑1 (MQ=60)
                                                                              TCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATG              <  SRR4302533.286637/94‑1 (MQ=60)
                                                                              TCGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATG              <  SRR4302533.3269102/94‑1 (MQ=60)
                                                                               CGGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGC             <  SRR4302533.5179196/94‑1 (MQ=60)
                                                                                GGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATACATGACTCATATCCTGTTAACCCGGCAATCATCTCCGCCGGATGCT            >  SRR4302533.168742/1‑94 (MQ=60)
                                                                                GGATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCT            <  SRR4302533.2905099/94‑1 (MQ=60)
                                                                                 GATGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTG           >  SRR4302533.3345029/1‑94 (MQ=60)
                                                                                   TGCGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGAC         <  SRR4302533.190452/94‑1 (MQ=60)
                                                                                     CGGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACAC       <  SRR4302533.958203/94‑1 (MQ=60)
                                                                                      GGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACA      >  SRR4302533.6116423/1‑94 (MQ=60)
                                                                                      GGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACA      <  SRR4302533.664531/94‑1 (MQ=60)
                                                                                      GGCGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACA      >  SRR4302533.1067644/1‑94 (MQ=60)
                                                                                        CGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAAT    >  SRR4302533.3090371/1‑94 (MQ=60)
                                                                                        CGC‑ATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAAT    >  SRR4302533.1747102/1‑94 (MQ=60)
                                                                                           CATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATAT  <  SRR4302533.2967540/94‑1 (MQ=60)
                                                                                           CATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATAT  >  SRR4302533.4998153/1‑94 (MQ=60)
                                                                                           CATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATAT  <  SRR4302533.4570867/94‑1 (MQ=60)
                                                                                           CATCCGACACAGTTCACCCCCTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATAT  <  SRR4302533.575767/94‑1 (MQ=60)
                                                                                           |                                                                                             
AGAGCATTACGCGAGTACAACGAAATGGTTGATGACTATAATAGCCTGCGTTAAACGTTTTTGATGATAAATGCAGTGTCGGATGCGGCGCGATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATAT  >  NC_000913/4085584‑4085768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑