Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,095,111 G→A intergenic (‑82/‑206) rhaB ← / → rhaS rhamnulokinase/RhaS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,095,1110GA99.8% 2359.6 / ‑6.2 643intergenic (‑82/‑206)rhaB/rhaSrhamnulokinase/RhaS
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (420/221);  total (421/222)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01

Only 100 of 648 total aligned reads displayed.

AAGGTCATAATGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAAT  >  NC_000913/4095022‑4095200
                                                                                         |                                                                                         
gtcgacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGcc                                                                                        >  1:7905781‑M2/12‑93 (MQ=255)
 tcgacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCa                                                                                       <  2:2803354‑M2/83‑1 (MQ=255)
  cgacatatgTTGATCCTGCTGAATGTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCAt                                                                                      <  2:6592434‑M2/84‑1 (MQ=255)
   gacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCAtt                                                                                     >  2:311639‑M2/9‑93 (MQ=255)
   gacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCAtt                                                                                     <  1:9687701‑M2/85‑1 (MQ=255)
   gacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCAtt                                                                                     >  2:4234073‑M2/9‑93 (MQ=255)
   gacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCAtt                                                                                     <  2:206882‑M2/85‑1 (MQ=255)
   gacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGACAtt                                                                                     >  1:5521758‑M2/9‑93 (MQ=255)
     catatgGTGATCCTGCTGAAGTTCATTACGACCAGTCTAAAAATCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTgg                                                                                   <  2:4989098‑M2/87‑1 (MQ=255)
       tatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCa                                                                                 >  1:524120‑M2/5‑93 (MQ=255)
       tatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCa                                                                                 >  1:5124755‑M2/5‑93 (MQ=255)
       tatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCa                                                                                 >  2:6080200‑M2/5‑93 (MQ=255)
       tatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCa                                                                                 >  2:5317437‑M2/5‑93 (MQ=255)
       tatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCa                                                                                 <  1:1382961‑M2/89‑1 (MQ=255)
        atgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCaa                                                                                <  1:3158158‑M2/90‑1 (MQ=255)
        atgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCaa                                                                                >  1:7223903‑M2/4‑93 (MQ=255)
        atgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCaa                                                                                >  2:4448292‑M2/4‑93 (MQ=255)
         tgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAAc                                                                               >  1:4331194‑M2/3‑93 (MQ=255)
              aTCGTGCTGAATTTCATTACGACCACTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAggg                                                                          <  2:7464017/93‑1 (MQ=255)
              aTCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAggg                                                                          >  1:8581942/1‑93 (MQ=255)
               tCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGa                                                                         >  2:9037578/1‑93 (MQ=255)
                ccTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGaa                                                                        <  2:6975953/93‑1 (MQ=255)
                ccTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGaa                                                                        >  1:6650601/1‑93 (MQ=255)
                ccTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCGCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGaa                                                                        <  2:7988340/93‑1 (MQ=255)
                  tgctgAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAg                                                                      <  1:5857883/93‑1 (MQ=255)
                  tgctgAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAg                                                                      >  2:1884616/1‑93 (MQ=255)
                    ctgAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGAt                                                                    >  2:2964633/1‑93 (MQ=255)
                      gAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGa                                                                  >  1:3035309/1‑93 (MQ=255)
                      gAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGa                                                                  >  2:8611140/1‑93 (MQ=255)
                         tttCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACg                                                               >  2:5466500/1‑93 (MQ=255)
                           tCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGtg                                                             <  1:1073331/93‑1 (MQ=255)
                             aTTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGTAACCAGGGAAAGATGAACGtgat                                                           >  2:7209338/1‑93 (MQ=255)
                             aTTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGtgat                                                           <  2:1130969/93‑1 (MQ=255)
                              ttACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGtgatg                                                          >  2:3622469/1‑93 (MQ=255)
                               tACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGtgatga                                                         <  1:6362461/93‑1 (MQ=255)
                               tACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGtgatga                                                         >  2:6636481/1‑93 (MQ=255)
                                   aCCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGtt                                                     >  1:4497172/1‑93 (MQ=255)
                                   aCCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGtt                                                     >  1:4229719/1‑93 (MQ=255)
                                   aCCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGtt                                                     >  1:9797769/1‑93 (MQ=255)
                                     cAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTca                                                   <  1:1754899/93‑1 (MQ=255)
                                        tCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACaa                                                >  2:5958832/1‑93 (MQ=255)
                                        tCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACaa                                                <  2:3703065/93‑1 (MQ=255)
                                        tCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACaa                                                >  1:811279/1‑93 (MQ=255)
                                        tCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACaa                                                >  2:2254202/1‑93 (MQ=255)
                                         cTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAAt                                               >  2:63744/1‑93 (MQ=255)
                                         cTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAAt                                               >  1:1290940/1‑93 (MQ=255)
                                          tAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAAtt                                              >  1:4088015/1‑93 (MQ=255)
                                          tAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAAtt                                              >  1:1448254/1‑93 (MQ=255)
                                          tAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAAtt                                              <  1:6128959/93‑1 (MQ=255)
                                           aaaaaGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAAttt                                             >  1:8223111/1‑93 (MQ=255)
                                            aaaaGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTg                                            >  2:885190/1‑93 (MQ=255)
                                            aaaaGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTg                                            >  1:3251784/1‑93 (MQ=255)
                                              aaGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCt                                          <  1:1128542/93‑1 (MQ=255)
                                              aaGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCt                                          <  1:6101000/93‑1 (MQ=255)
                                              aaGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCt                                          <  1:9081617/93‑1 (MQ=255)
                                               aGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTg                                         <  2:825365/93‑1 (MQ=255)
                                                gcgcCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGa                                        <  2:1000508/93‑1 (MQ=255)
                                                gcgcCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGa                                        >  2:8429673/1‑93 (MQ=255)
                                                    cTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATtg                                    <  2:5646482/93‑1 (MQ=255)
                                                     tGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATtgt                                   <  2:7795102/93‑1 (MQ=255)
                                                     tGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATtgt                                   >  2:2178000/1‑93 (MQ=255)
                                                       aaTTCGCGACCTTCTCGTTACTGACAGGAAAATTAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTgtgg                                 <  2:4829630/93‑1 (MQ=255)
                                                       aaTTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTgtgg                                 <  1:249577/93‑1 (MQ=255)
                                                        aTTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTgtggt                                >  2:1684314/1‑93 (MQ=255)
                                                         ttCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTgtggtg                               >  1:7399767/1‑93 (MQ=255)
                                                           cgcgACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGgtgat                             <  2:943909/93‑1 (MQ=255)
                                                            gcgACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGgtgatg                            >  1:6509191/1‑93 (MQ=255)
                                                              gACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGgtgatgtg                          <  1:4978598/93‑1 (MQ=255)
                                                               aCCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGgtgatgtga                         <  2:2439370/93‑1 (MQ=255)
                                                               aCCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGgtgatgtga                         <  1:4019018/93‑1 (MQ=255)
                                                               aCCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGgtgatgtga                         >  2:4669436/1‑93 (MQ=255)
                                                                ccTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGgtgatgtgat                        <  2:6217550/93‑1 (MQ=255)
                                                                 cTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGtgatgtgatg                       >  1:2313104/1‑93 (MQ=255)
                                                                  ttCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGc                      >  2:9843257/1‑93 (MQ=255)
                                                                  ttCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGc                      >  2:146471/1‑93 (MQ=255)
                                                                  ttCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGc                      >  1:8999902/1‑93 (MQ=255)
                                                                   tctcGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCt                     >  1:3531836/1‑93 (MQ=255)
                                                                   tctcGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCt                     >  1:537909/1‑93 (MQ=255)
                                                                    ctcGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTc                    >  1:2667372/1‑93 (MQ=255)
                                                                     tcGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCa                   >  2:5173144/1‑93 (MQ=255)
                                                                      cGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCAc                  <  1:2902232/93‑1 (MQ=255)
                                                                      cGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCAc                  <  1:9276049/93‑1 (MQ=255)
                                                                        ttACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCg                >  1:4625117/1‑93 (MQ=255)
                                                                        ttACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCg                >  1:950617/1‑93 (MQ=255)
                                                                         tACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGc               >  2:9636438/1‑93 (MQ=255)
                                                                         tACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGc               >  1:6245357/1‑93 (MQ=255)
                                                                          aCTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCa              >  1:8822719/1‑93 (MQ=255)
                                                                          aCTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCa              >  2:2552788/1‑93 (MQ=255)
                                                                           cTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCAt             >  2:3398613/1‑93 (MQ=255)
                                                                           cTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCAt             <  1:2200144/93‑1 (MQ=255)
                                                                            tGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCAtt            >  1:7590617/1‑93 (MQ=255)
                                                                            tGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCAtt            >  2:9281243/1‑93 (MQ=255)
                                                                                 ggAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTg       >  2:1265891/1‑93 (MQ=255)
                                                                                 ggAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTg       >  1:6977783/1‑93 (MQ=255)
                                                                                   aaaaTGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGaa     >  1:160862/1‑93 (MQ=255)
                                                                                   aaaaTGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGaa     <  2:4131169/93‑1 (MQ=255)
                                                                                    aaaTGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGaaa    <  2:7615835/93‑1 (MQ=255)
                                                                                      aTGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAAt  >  1:5657021/1‑93 (MQ=255)
                                                                                      aTGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAAt  >  1:3715279/1‑93 (MQ=255)
                                                                                      aTGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAAt  <  1:7748307/93‑1 (MQ=255)
                                                                                         |                                                                                         
AAGGTCATAATGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAAT  >  NC_000913/4095022‑4095200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 354 total aligned reads displayed.
GCGAAAGGTCATAATGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCAC  >  NC_000913/4095018‑4095204
                                                                                             |                                                                                             
tagagtcgacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGA                                                                                               >  SRR4302540.1072214/16‑94 (MQ=60)
tagagtcgacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGA                                                                                               <  SRR4302540.2200264/79‑1 (MQ=60)
tagagtcgacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTAAATTCGCGACCTTCTCGTTACTGACAGGAAAATGA                                                                                               >  SRR4302540.253051/16‑94 (MQ=60)
cagagccggcatatggtgatcctgggtaacttcattccctCCAGTCTAAAAAGCGCCTGAATTGGCCTCCTTCTGGTTACTGACAGGAAAATGA                                                                                               <  SRR4302540.8699099/54‑1 (MQ=34)
 agagtcgacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAG                                                                                              <  SRR4302540.2173103/80‑1 (MQ=60)
  gagtcgacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGC                                                                                             >  SRR4302540.5405229/14‑94 (MQ=60)
   agtcgacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCC                                                                                            <  SRR4302540.7142086/82‑1 (MQ=60)
    gtcgacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCA                                                                                           <  SRR4302540.569672/83‑1 (MQ=60)
    gtcgacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCA                                                                                           >  SRR4302540.9001522/12‑94 (MQ=60)
     tcgacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCAT                                                                                          <  SRR4302540.6194079/84‑1 (MQ=60)
     tcgacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCAT                                                                                          >  SRR4302540.5465367/11‑94 (MQ=60)
     gcgacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCAT                                                                                          <  SRR4302540.3521499/84‑1 (MQ=60)
      cgacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATT                                                                                         <  SRR4302540.3089363/85‑1 (MQ=60)
      cgacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATT                                                                                         <  SRR4302540.745559/85‑1 (MQ=60)
      cgacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATT                                                                                         >  SRR4302540.7935253/10‑94 (MQ=60)
      cgacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATT                                                                                         <  SRR4302540.109905/85‑1 (MQ=60)
      cgacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGACATT                                                                                         >  SRR4302540.5582563/10‑94 (MQ=60)
       gacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTG                                                                                        <  SRR4302540.9801658/86‑1 (MQ=60)
       gacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTG                                                                                        <  SRR4302540.2930168/86‑1 (MQ=60)
        acatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGG                                                                                       >  SRR4302540.9340701/8‑94 (MQ=60)
         CATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGC                                                                                      >  SRR4302540.6457973/1‑94 (MQ=60)
          atatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCA                                                                                     >  SRR4302540.9295260/6‑94 (MQ=60)
          atatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCA                                                                                     >  SRR4302540.6018210/6‑94 (MQ=60)
          atatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCA                                                                                     >  SRR4302540.919126/6‑94 (MQ=60)
          atatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCA                                                                                     >  SRR4302540.529768/6‑94 (MQ=60)
          atatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCA                                                                                     >  SRR4302540.5180261/6‑94 (MQ=60)
          atatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCA                                                                                     <  SRR4302540.2450104/89‑1 (MQ=60)
           tatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAC                                                                                    >  SRR4302540.586323/5‑94 (MQ=60)
           tatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAA                                                                                    <  SRR4302540.1397725/90‑1 (MQ=60)
           tatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAA                                                                                    >  SRR4302540.7304439/5‑94 (MQ=60)
            atgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAAC                                                                                   >  SRR4302540.4378339/4‑94 (MQ=60)
            atgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAAC                                                                                   <  SRR4302540.3192527/91‑1 (MQ=60)
             tgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGCCCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACC                                                                                  >  SRR4302540.2588113/3‑94 (MQ=60)
             tgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACC                                                                                  <  SRR4302540.6621502/92‑1 (MQ=60)
              GGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCA                                                                                 >  SRR4302540.2630147/1‑94 (MQ=60)
              GGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCA                                                                                 >  SRR4302540.6053397/1‑94 (MQ=60)
               GTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAG                                                                                >  SRR4302540.2747926/1‑94 (MQ=60)
               GTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAG                                                                                >  SRR4302540.7555650/1‑94 (MQ=60)
                TGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGG                                                                               >  SRR4302540.1283493/1‑94 (MQ=60)
                 GATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGG                                                                              >  SRR4302540.9940246/1‑94 (MQ=60)
                 GATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGG                                                                              <  SRR4302540.5171159/94‑1 (MQ=60)
                 GATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGG                                                                              >  SRR4302540.8681703/1‑94 (MQ=60)
                  ATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGCCCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGA                                                                             <  SRR4302540.7346465/94‑1 (MQ=60)
                  ATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGA                                                                             >  SRR4302540.6445206/1‑94 (MQ=60)
                   TCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAA                                                                            <  SRR4302540.1613192/94‑1 (MQ=60)
                     CTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAG                                                                          >  SRR4302540.7404597/1‑94 (MQ=60)
                      TGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGA                                                                         >  SRR4302540.4083336/1‑94 (MQ=60)
                       GCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGAT                                                                        <  SRR4302540.2308696/94‑1 (MQ=60)
                       GCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGAT                                                                        >  SRR4302540.8122282/1‑94 (MQ=60)
                       GCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGAT                                                                        <  SRR4302540.8004002/94‑1 (MQ=60)
                        CTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATG                                                                       >  SRR4302540.3882317/1‑94 (MQ=60)
                        CTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATG                                                                       >  SRR4302540.4505933/1‑94 (MQ=60)
                         TGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGA                                                                      >  SRR4302540.3068621/1‑94 (MQ=60)
                         TGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGA                                                                      >  SRR4302540.2267606/1‑94 (MQ=60)
                         TGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGA                                                                      >  SRR4302540.5570045/1‑94 (MQ=60)
                         TGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGA                                                                      >  SRR4302540.8300503/1‑94 (MQ=60)
                          GAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAA                                                                     >  SRR4302540.498527/1‑94 (MQ=60)
                           AATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAAC                                                                    >  SRR4302540.6388024/1‑94 (MQ=60)
                            ATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACG                                                                   >  SRR4302540.9188448/1‑94 (MQ=60)
                            ATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACG                                                                   >  SRR4302540.1427713/1‑94 (MQ=60)
                            ATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACG                                                                   <  SRR4302540.8952372/94‑1 (MQ=60)
                             TTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGT                                                                  >  SRR4302540.5311356/1‑94 (MQ=60)
                             TTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGT                                                                  >  SRR4302540.9837976/1‑94 (MQ=60)
                              TTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTG                                                                 >  SRR4302540.9911495/1‑94 (MQ=60)
                               TCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGA                                                                >  SRR4302540.5265589/1‑94 (MQ=60)
                               TCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGA                                                                <  SRR4302540.1084292/94‑1 (MQ=60)
                               TCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGA                                                                >  SRR4302540.2443899/1‑94 (MQ=60)
                               TCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGA                                                                >  SRR4302540.371623/1‑94 (MQ=60)
                                CATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGAT                                                               <  SRR4302540.8238659/94‑1 (MQ=60)
                                CATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGAT                                                               >  SRR4302540.7556828/1‑94 (MQ=60)
                                CATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGCGAT                                                               >  SRR4302540.109203/1‑94 (MQ=60)
                                   TACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGAT                                                            <  SRR4302540.6433309/94‑1 (MQ=60)
                                     CGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGT                                                          >  SRR4302540.1174248/1‑94 (MQ=60)
                                     CGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGT                                                          >  SRR4302540.1230119/1‑94 (MQ=60)
                                      GACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTT                                                         >  SRR4302540.1529834/1‑94 (MQ=60)
                                      GACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTT                                                         >  SRR4302540.7756248/1‑94 (MQ=60)
                                      GACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGGGATGATGTT                                                         >  SRR4302540.1529833/1‑94 (MQ=60)
                                       GCCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTC                                                        <  SRR4302540.8356881/94‑1 (MQ=60)
                                       ACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTC                                                        <  SRR4302540.9175436/94‑1 (MQ=60)
                                       ACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTC                                                        <  SRR4302540.7878146/94‑1 (MQ=60)
                                       ACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTC                                                        >  SRR4302540.1734479/1‑94 (MQ=60)
                                       ACCAGTCTAAAAAGCGCCTGAATTCGCGACCTCCTCGTTACTGACAGGAAAATGAGCCATTGGCAACAAGGGAAAGATGAACGTGATGATGTTC                                                        <  SRR4302540.7878450/94‑1 (MQ=60)
                                        CCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCA                                                       >  SRR4302540.9742541/1‑94 (MQ=60)
                                        CCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCA                                                       <  SRR4302540.9399227/94‑1 (MQ=60)
                                        CCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCA                                                       >  SRR4302540.8196441/1‑94 (MQ=60)
                                         CAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCAC                                                      >  SRR4302540.6767569/1‑94 (MQ=60)
                                         CAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCAC                                                      >  SRR4302540.9307899/1‑94 (MQ=60)
                                         CAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCAC                                                      <  SRR4302540.1773915/94‑1 (MQ=60)
                                          AGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACA                                                     >  SRR4302540.1484008/1‑94 (MQ=60)
                                          AGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACA                                                     >  SRR4302540.4329225/1‑94 (MQ=60)
                                           GTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAT                                                    >  SRR4302540.1648164/1‑94 (MQ=60)
                                           GTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAA                                                    <  SRR4302540.7735263/94‑1 (MQ=60)
                                           GTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAA                                                    >  SRR4302540.9443482/1‑94 (MQ=60)
                                           GTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAA                                                    >  SRR4302540.3230731/1‑94 (MQ=60)
                                           GTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAA                                                    >  SRR4302540.298129/1‑94 (MQ=60)
                                           GTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAA                                                    >  SRR4302540.186258/1‑94 (MQ=60)
                                           GTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAA                                                    >  SRR4302540.4556970/1‑94 (MQ=60)
                                           GTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAA                                                    >  SRR4302540.6494336/1‑94 (MQ=60)
                                            TCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAAT                                                   <  SRR4302540.8632794/94‑1 (MQ=60)
                                            TCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAAT                                                   >  SRR4302540.1304284/1‑94 (MQ=60)
                                             CTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGCGCCATTGGCAACCAGGGACAGCTGAACGTGATGATGTTCACAATT                                                  >  SRR4302540.1464000/1‑94 (MQ=60)
                                             CTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATT                                                  >  SRR4302540.6327999/1‑94 (MQ=60)
                                             CTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATT                                                  <  SRR4302540.5948433/94‑1 (MQ=60)
                                             CTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATT                                                  >  SRR4302540.4132100/1‑94 (MQ=60)
                                              TAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTT                                                 >  SRR4302540.8318702/1‑94 (MQ=60)
                                              TAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTT                                                 <  SRR4302540.6197226/94‑1 (MQ=60)
                                               AAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTG                                                <  SRR4302540.589871/94‑1 (MQ=60)
                                               AAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTG                                                <  SRR4302540.7197652/94‑1 (MQ=60)
                                               AAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTG                                                >  SRR4302540.3287216/1‑94 (MQ=60)
                                               AAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTG                                                >  SRR4302540.2393817/1‑94 (MQ=60)
                                               AAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTG                                                >  SRR4302540.4303652/1‑94 (MQ=60)
                                                AAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAACGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGC                                               >  SRR4302540.5064966/1‑94 (MQ=60)
                                                 AAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCT                                              <  SRR4302540.8442658/94‑1 (MQ=60)
                                                  AAGGGCCGGAATTCGCGACCCTCTCGTTACGGACAGGAAAATGAGCCATTGGCACCCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTG                                             <  SRR4302540.6168932/94‑1 (MQ=60)
                                                  AAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTG                                             <  SRR4302540.3248221/94‑1 (MQ=60)
                                                  AAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTG                                             <  SRR4302540.1140284/94‑1 (MQ=60)
                                                  AAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTG                                             >  SRR4302540.6716302/1‑94 (MQ=60)
                                                    GCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAA                                           <  SRR4302540.7821563/94‑1 (MQ=60)
                                                    GCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAA                                           <  SRR4302540.6283690/94‑1 (MQ=60)
                                                     CGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAAT                                          <  SRR4302540.9679221/94‑1 (MQ=60)
                                                     CGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGATGAAT                                          >  SRR4302540.4026776/1‑94 (MQ=60)
                                                      GCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATT                                         >  SRR4302540.1999621/1‑94 (MQ=60)
                                                       CCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTG                                        >  SRR4302540.5993410/1‑94 (MQ=60)
                                                        CTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGT                                       >  SRR4302540.4632641/1‑94 (MQ=60)
                                                        CTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGT                                       >  SRR4302540.4171084/1‑94 (MQ=60)
                                                        CTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGT                                       >  SRR4302540.4240929/1‑94 (MQ=60)
                                                        CTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGT                                       >  SRR4302540.4410990/1‑94 (MQ=60)
                                                        CTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGT                                       >  SRR4302540.4721392/1‑94 (MQ=60)
                                                         TGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTG                                      >  SRR4302540.172987/1‑94 (MQ=60)
                                                         TGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTG                                      >  SRR4302540.6173348/1‑94 (MQ=60)
                                                         TGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTG                                      <  SRR4302540.3540889/94‑1 (MQ=60)
                                                         TGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTG                                      >  SRR4302540.3797409/1‑94 (MQ=60)
                                                          GAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGG                                     <  SRR4302540.9989972/94‑1 (MQ=60)
                                                           AATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGT                                    <  SRR4302540.1323730/94‑1 (MQ=60)
                                                           AATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGT                                    >  SRR4302540.6924798/1‑94 (MQ=60)
                                                            ATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTG                                   >  SRR4302540.7482204/1‑94 (MQ=60)
                                                              TCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGAT                                 <  SRR4302540.8219040/94‑1 (MQ=60)
                                                              TCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGAT                                 <  SRR4302540.8796866/94‑1 (MQ=60)
                                                               CGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATG                                >  SRR4302540.6581521/1‑94 (MQ=60)
                                                                GCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGT                               <  SRR4302540.5513875/94‑1 (MQ=60)
                                                                 CGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCTGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTG                              <  SRR4302540.2291610/94‑1 (MQ=60)
                                                                 CGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTG                              <  SRR4302540.3009032/94‑1 (MQ=60)
                                                                  GACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGA                             >  SRR4302540.1354441/1‑94 (MQ=60)
                                                                  GACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGA                             <  SRR4302540.5032488/94‑1 (MQ=60)
                                                                   ACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGAT                            <  SRR4302540.7033763/94‑1 (MQ=60)
                                                                   ACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGAT                            <  SRR4302540.4062311/94‑1 (MQ=60)
                                                                   ACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGAT                            <  SRR4302540.99842/94‑1 (MQ=60)
                                                                   ACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGAT                            <  SRR4302540.6612265/94‑1 (MQ=60)
                                                                    CCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATG                           <  SRR4302540.6944779/94‑1 (MQ=60)
                                                                    CCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATG                           >  SRR4302540.2338001/1‑94 (MQ=60)
                                                                     CTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGC                          >  SRR4302540.9107880/1‑94 (MQ=60)
                                                                     CTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGC                          >  SRR4302540.5337345/1‑94 (MQ=60)
                                                                     CTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGC                          <  SRR4302540.8877775/94‑1 (MQ=60)
                                                                     CTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGC                          >  SRR4302540.1431834/1‑94 (MQ=60)
                                                                     CTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGC                          >  SRR4302540.462355/1‑94 (MQ=60)
                                                                      TTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCT                         >  SRR4302540.3570387/1‑94 (MQ=60)
                                                                      TTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCT                         >  SRR4302540.1376088/1‑94 (MQ=60)
                                                                      TTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCT                         >  SRR4302540.563440/1‑94 (MQ=60)
                                                                      TTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCT                         >  SRR4302540.6208760/1‑94 (MQ=60)
                                                                      TTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGGGATGCT                         >  SRR4302540.9756345/1‑94 (MQ=60)
                                                                       TCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTC                        >  SRR4302540.2696278/1‑94 (MQ=60)
                                                                        CTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCA                       >  SRR4302540.4224371/1‑94 (MQ=60)
                                                                        CTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCA                       >  SRR4302540.5210666/1‑94 (MQ=60)
                                                                        CTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCA                       >  SRR4302540.3456574/1‑94 (MQ=60)
                                                                         TCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCAC                      <  SRR4302540.6320260/94‑1 (MQ=60)
                                                                          CGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACC                     <  SRR4302540.903794/94‑1 (MQ=60)
                                                                          CGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACC                     <  SRR4302540.2933907/94‑1 (MQ=60)
                                                                           GTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCG                    >  SRR4302540.4674677/1‑94 (MQ=60)
                                                                           GTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCG                    >  SRR4302540.4924517/1‑94 (MQ=60)
                                                                           GTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCG                    >  SRR4302540.3171520/1‑94 (MQ=60)
                                                                             TACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCA                  >  SRR4302540.4399134/1‑94 (MQ=60)
                                                                             TACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCA                  >  SRR4302540.2812208/1‑94 (MQ=60)
                                                                              ACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCAT                 >  SRR4302540.9537696/1‑94 (MQ=60)
                                                                               CTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATT                <  SRR4302540.4078405/94‑1 (MQ=60)
                                                                               CTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATT                >  SRR4302540.7675878/1‑94 (MQ=60)
                                                                                TGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTT               >  SRR4302540.3187580/1‑94 (MQ=60)
                                                                                TGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTT               >  SRR4302540.5647881/1‑94 (MQ=60)
                                                                                 GACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTC              <  SRR4302540.9179394/94‑1 (MQ=60)
                                                                                 GACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTC              <  SRR4302540.2623030/94‑1 (MQ=60)
                                                                                  ACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCC             <  SRR4302540.8112492/94‑1 (MQ=60)
                                                                                   CAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCT            >  SRR4302540.5246912/1‑94 (MQ=60)
                                                                                   CAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCT            <  SRR4302540.3625364/94‑1 (MQ=60)
                                                                                    AGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTG           >  SRR4302540.4329107/1‑94 (MQ=60)
                                                                                    AGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTG           <  SRR4302540.9615306/94‑1 (MQ=60)
                                                                                     GGAAAATGTGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGA          <  SRR4302540.987266/94‑1 (MQ=60)
                                                                                     GGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGA          >  SRR4302540.8900929/1‑94 (MQ=60)
                                                                                     GGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGA          >  SRR4302540.5828068/1‑94 (MQ=60)
                                                                                       AAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAA        >  SRR4302540.4527290/1‑94 (MQ=60)
                                                                                       AAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAA        >  SRR4302540.7069834/1‑94 (MQ=60)
                                                                                         AATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAAT      <  SRR4302540.6310672/94‑1 (MQ=60)
                                                                                         AATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAAT      >  SRR4302540.4133371/1‑94 (MQ=60)
                                                                                         AATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAAT      >  SRR4302540.5719314/1‑94 (MQ=60)
                                                                                          ATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATT     >  SRR4302540.3826058/1‑94 (MQ=60)
                                                                                          ATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATT     >  SRR4302540.2493572/1‑94 (MQ=60)
                                                                                          ATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATT     <  SRR4302540.7838772/94‑1 (MQ=60)
                                                                                           TGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTC    <  SRR4302540.3639652/94‑1 (MQ=60)
                                                                                           TGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTC    >  SRR4302540.352971/1‑94 (MQ=60)
                                                                                            GAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCA   >  SRR4302540.2860518/1‑94 (MQ=60)
                                                                                             AGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAATTTCAC  >  SRR4302540.5506517/1‑94 (MQ=60)
                                                                                             AGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCAC  <  SRR4302540.2133334/94‑1 (MQ=60)
                                                                                             |                                                                                             
GCGAAAGGTCATAATGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCAC  >  NC_000913/4095018‑4095204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑