Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,095,111 G→A intergenic (‑82/‑206) rhaB ← / → rhaS rhamnulokinase/RhaS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,095,1110GA100.0% 946.9 / NA 252intergenic (‑82/‑206)rhaB/rhaSrhamnulokinase/RhaS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (139/113);  total (139/113)

Only 100 of 255 total aligned reads displayed.

AAAGGTCATAATGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAAT  >  NC_000913/4095021‑4095200
                                                                                          |                                                                                         
agtcgacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGc                                                                                         <  2:470887‑M2/81‑1 (MQ=255)
 gtcgacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGcc                                                                                        >  1:892889‑M2/12‑93 (MQ=255)
  tcgacatatgGTGATCCTGCTTAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCa                                                                                       <  1:4911323‑M2/83‑1 (MQ=255)
   cgacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCAt                                                                                      <  2:5334415‑M2/84‑1 (MQ=255)
   cgacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCAt                                                                                      <  2:2878496‑M2/84‑1 (MQ=255)
    gacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCAtt                                                                                     <  1:2065837‑M2/85‑1 (MQ=255)
    gacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCAtt                                                                                     >  2:13369‑M2/9‑93 (MQ=255)
     acatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTg                                                                                    >  1:5530097‑M2/8‑93 (MQ=255)
     acatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTg                                                                                    <  1:441237‑M2/86‑1 (MQ=255)
      catatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTgg                                                                                   <  2:227823‑M2/87‑1 (MQ=255)
       atatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGc                                                                                  >  2:1474704‑M2/6‑93 (MQ=255)
        tatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCCTTTGCa                                                                                 >  2:6071338‑M2/5‑93 (MQ=255)
        tatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCa                                                                                 >  1:5844338‑M2/5‑93 (MQ=255)
        tatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCa                                                                                 >  2:6001189‑M2/5‑93 (MQ=255)
        tatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCa                                                                                 >  1:2002752‑M2/5‑93 (MQ=255)
           gGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAAcc                                                                              <  2:1963178‑M2/92‑1 (MQ=255)
            gtgATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCa                                                                             >  2:5801494/1‑93 (MQ=255)
            gtgATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCa                                                                             >  1:4555178/1‑93 (MQ=255)
            gtgATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCa                                                                             <  2:85700/93‑1 (MQ=255)
             tgATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAg                                                                            <  2:2276353/93‑1 (MQ=255)
              gATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAgg                                                                           <  1:4601372/93‑1 (MQ=255)
               aTCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAggg                                                                          <  1:5321585/93‑1 (MQ=255)
                tCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGa                                                                         <  1:5553555/93‑1 (MQ=255)
                tCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGa                                                                         >  2:3724604/1‑93 (MQ=255)
                 ccTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGaa                                                                        >  1:276142/1‑93 (MQ=255)
                  ctgctgAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGaaa                                                                       >  2:4740209/1‑93 (MQ=255)
                  ctgctgAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGaaa                                                                       >  1:4636534/1‑93 (MQ=255)
                   tgctgAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCCTTGGCAACCAGGGaaaa                                                                      >  2:2392570/1‑92 (MQ=255)
                        aaTTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGaa                                                                 <  1:5003413/93‑1 (MQ=255)
                         aTTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAAc                                                                <  2:1925924/93‑1 (MQ=255)
                          tttCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACg                                                               <  2:1894129/93‑1 (MQ=255)
                             cATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGtga                                                            >  1:329205/1‑93 (MQ=255)
                             cATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGtga                                                            >  1:1963646/1‑93 (MQ=255)
                              aTTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGtgat                                                           >  1:3790878/1‑93 (MQ=255)
                                   gACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGt                                                      >  2:3405448/1‑93 (MQ=255)
                                   gACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGt                                                      >  2:4431981/1‑93 (MQ=255)
                                   gACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGt                                                      >  1:2948391/1‑93 (MQ=255)
                                    aCCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGtt                                                     <  1:1055936/93‑1 (MQ=255)
                                     ccAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTc                                                    >  2:3272435/1‑93 (MQ=255)
                                     ccAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTc                                                    >  2:4017981/1‑93 (MQ=255)
                                     ccAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTc                                                    >  2:5578562/1‑93 (MQ=255)
                                     ccAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTc                                                    <  1:636548/93‑1 (MQ=255)
                                      cAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACAAGGGAAAGATGAACGTGATGATGTTca                                                   >  1:3974199/1‑93 (MQ=255)
                                        gTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTcaca                                                 >  2:4850610/1‑93 (MQ=255)
                                         tCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACaa                                                <  2:5380869/93‑1 (MQ=255)
                                         tCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACaa                                                >  1:1090859/1‑93 (MQ=255)
                                         tCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACaa                                                >  1:3494176/1‑93 (MQ=255)
                                         tCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACaa                                                >  1:5159126/1‑93 (MQ=255)
                                          cTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAAt                                               <  2:5249314/93‑1 (MQ=255)
                                            aaaaaGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAAttt                                             <  2:2134058/93‑1 (MQ=255)
                                             aaaaGCGCCTGAATTCGCGACCTTCTCGTTACCGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTg                                            <  2:3416302/93‑1 (MQ=255)
                                              aaaGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGc                                           <  1:728660/93‑1 (MQ=255)
                                                aGCGCCTGAATTCGCGAGCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTg                                         >  2:3371243/1‑93 (MQ=255)
                                                aGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTg                                         >  1:2660828/1‑93 (MQ=255)
                                                   gcCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAAt                                      >  1:2323557/1‑93 (MQ=255)
                                                   gcCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAAt                                      <  2:2758925/93‑1 (MQ=255)
                                                    ccTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGCGATGATGTTCACAATTTGCTGaaat                                     >  2:1129623/1‑91 (MQ=255)
                                                    ccTGAATTAGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGTAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAAtt                                     >  2:1043582/1‑93 (MQ=255)
                                                     cTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATtg                                    >  1:3569114/1‑93 (MQ=255)
                                                     cTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATtg                                    >  1:3890620/1‑93 (MQ=255)
                                                      tGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATtgt                                   >  2:4310008/1‑93 (MQ=255)
                                                      tGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATtgt                                   <  2:4390381/93‑1 (MQ=255)
                                                      tGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATtgt                                   <  1:370648/93‑1 (MQ=255)
                                                      tGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATtgt                                   >  2:4616227/1‑93 (MQ=255)
                                                      tGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATtgt                                   >  1:2402650/1‑93 (MQ=255)
                                                      tGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGGTCACAATTTGCTGAATtgt                                   >  1:2467252/1‑93 (MQ=255)
                                                       gAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATtgtg                                  <  1:613183/93‑1 (MQ=255)
                                                       gAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATtgtg                                  >  2:1506763/1‑93 (MQ=255)
                                                        aaTTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTgtgg                                 <  2:2449458/93‑1 (MQ=255)
                                                         aTTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTgtggt                                <  1:4214352/93‑1 (MQ=255)
                                                          ttCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTgtggtg                               >  1:5911520/1‑93 (MQ=255)
                                                          ttCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTgtggtg                               >  1:4026813/1‑93 (MQ=255)
                                                           tCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGgtga                              <  2:6238303/93‑1 (MQ=255)
                                                            cgcgACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGgtgat                             <  1:47543/93‑1 (MQ=255)
                                                            cgcgACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGgtgat                             <  2:165554/93‑1 (MQ=255)
                                                              cgACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGgtgatgt                           >  2:1815207/1‑93 (MQ=255)
                                                              cgACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGgtgatgt                           <  1:3553794/93‑1 (MQ=255)
                                                              cgACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGgtgatgt                           <  2:3817235/93‑1 (MQ=255)
                                                               gACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGgtgatgtg                          >  2:710185/1‑93 (MQ=255)
                                                                aCCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGgtgatgtga                         <  1:3427663/93‑1 (MQ=255)
                                                                  cTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGtgatgtgatg                       <  2:4136671/93‑1 (MQ=255)
                                                                  cTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGtgatgtgatg                       >  1:3347604/1‑93 (MQ=255)
                                                                   ttCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGc                      >  1:6125778/1‑93 (MQ=255)
                                                                      tcGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCa                   >  1:2183099/1‑93 (MQ=255)
                                                                      tcGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCa                   >  1:319023/1‑93 (MQ=255)
                                                                      tcGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCa                   <  2:2520786/93‑1 (MQ=255)
                                                                          tACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGc               >  2:5901181/1‑93 (MQ=255)
                                                                          tACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGc               >  2:5432327/1‑93 (MQ=255)
                                                                           aCTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCa              >  2:5151339/1‑93 (MQ=255)
                                                                            cTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCAt             >  2:2608202/1‑93 (MQ=255)
                                                                              gACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCAttt           >  1:1387026/1‑93 (MQ=255)
                                                                              gACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCAttt           >  1:2720411/1‑93 (MQ=255)
                                                                              gACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCAttt           <  2:307679/93‑1 (MQ=255)
                                                                                  ggAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTg       <  2:2033813/93‑1 (MQ=255)
                                                                                     aaaTGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGaaa    >  1:4721119/1‑93 (MQ=255)
                                                                                      aaTGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGaaaa   <  2:5540113/93‑1 (MQ=255)
                                                                                      aaTGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGaaaa   <  2:1562527/93‑1 (MQ=255)
                                                                                      aaTGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGaaaa   >  1:4286868/1‑93 (MQ=255)
                                                                                       aTGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAAt  >  1:5700187/1‑93 (MQ=255)
                                                                                       aTGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAAt  <  1:1474704/93‑1 (MQ=255)
                                                                                          |                                                                                         
AAAGGTCATAATGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAAT  >  NC_000913/4095021‑4095200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGAAAGGTCATAATGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCAC  >  NC_000913/4095019‑4095204
                                                                                            |                                                                                             
agagtcgacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAG                                                                                              >  SRR4302533.5672324/15‑94 (MQ=60)
agagtcgacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAG                                                                                              <  SRR4302533.1155233/80‑1 (MQ=60)
agagtcgacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAG                                                                                              >  SRR4302533.1304021/15‑94 (MQ=60)
 gagtcgacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGC                                                                                             >  SRR4302533.3493405/14‑94 (MQ=60)
 gagtcgacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGC                                                                                             >  SRR4302533.3252944/14‑94 (MQ=60)
  agtcgacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCC                                                                                            >  SRR4302533.898137/13‑94 (MQ=60)
   gtcgacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCA                                                                                           >  SRR4302533.3582612/12‑94 (MQ=60)
    tcgacatatgGTGATCCTGCTTAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCAT                                                                                          <  SRR4302533.4941076/84‑1 (MQ=60)
    tcgacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCAT                                                                                          <  SRR4302533.2138030/84‑1 (MQ=60)
     cgacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATT                                                                                         >  SRR4302533.3051767/10‑94 (MQ=60)
     cgacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATT                                                                                         >  SRR4302533.4013925/10‑94 (MQ=60)
     cgacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATT                                                                                         >  SRR4302533.4017805/10‑94 (MQ=60)
     cgacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATT                                                                                         >  SRR4302533.5027299/10‑94 (MQ=60)
      gacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTG                                                                                        <  SRR4302533.892671/86‑1 (MQ=60)
      gacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTG                                                                                        >  SRR4302533.5563480/9‑94 (MQ=60)
      gacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTG                                                                                        <  SRR4302533.5866880/86‑1 (MQ=60)
      gacatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTG                                                                                        <  SRR4302533.2078568/86‑1 (MQ=60)
       acatatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGG                                                                                       <  SRR4302533.443872/87‑1 (MQ=60)
        CATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGC                                                                                      <  SRR4302533.1429632/94‑1 (MQ=60)
         atatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCA                                                                                     >  SRR4302533.3356158/6‑94 (MQ=60)
         atatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCA                                                                                     >  SRR4302533.5879671/6‑94 (MQ=60)
         atatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCA                                                                                     <  SRR4302533.6286253/89‑1 (MQ=60)
         atatgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCA                                                                                     >  SRR4302533.2015089/6‑94 (MQ=60)
           atgGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAAC                                                                                   >  SRR4302533.2587071/4‑94 (MQ=60)
             GGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCA                                                                                 <  SRR4302533.5929476/94‑1 (MQ=60)
             GGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCA                                                                                 >  SRR4302533.4582806/1‑94 (MQ=60)
             GGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCA                                                                                 <  SRR4302533.4899555/94‑1 (MQ=60)
              GTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAG                                                                                >  SRR4302533.2567220/1‑94 (MQ=60)
                GATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGG                                                                              <  SRR4302533.4629249/94‑1 (MQ=60)
                 ATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGA                                                                             <  SRR4302533.5353476/94‑1 (MQ=60)
                  TCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAA                                                                            >  SRR4302533.277824/1‑94 (MQ=60)
                  TCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAA                                                                            <  SRR4302533.5587092/94‑1 (MQ=60)
                   CCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAA                                                                           >  SRR4302533.4269588/1‑94 (MQ=60)
                   CCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAA                                                                           >  SRR4302533.4664629/1‑94 (MQ=60)
                    CTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAG                                                                          >  SRR4302533.4358727/1‑94 (MQ=60)
                     TGGTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGA                                                                         >  SRR4302533.465559/1‑94 (MQ=60)
                     TGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGA                                                                         >  SRR4302533.3981593/1‑94 (MQ=60)
                       CTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATG                                                                       >  SRR4302533.2470759/1‑94 (MQ=60)
                        TGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGA                                                                      >  SRR4302533.4850571/1‑94 (MQ=60)
                         GAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAA                                                                     >  SRR4302533.3643420/1‑94 (MQ=60)
                          AATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAAC                                                                    <  SRR4302533.5033738/94‑1 (MQ=60)
                          AATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAAC                                                                    <  SRR4302533.2772223/94‑1 (MQ=60)
                           ATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACG                                                                   >  SRR4302533.2778321/1‑94 (MQ=60)
                           ATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACG                                                                   >  SRR4302533.4615213/1‑94 (MQ=60)
                             TTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTG                                                                 <  SRR4302533.2914764/94‑1 (MQ=60)
                             TTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTG                                                                 >  SRR4302533.2036012/1‑94 (MQ=60)
                             TTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTG                                                                 >  SRR4302533.4263668/1‑94 (MQ=60)
                             TTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTG                                                                 >  SRR4302533.5430568/1‑94 (MQ=60)
                              TCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGA                                                                >  SRR4302533.331213/1‑94 (MQ=60)
                              TCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGA                                                                >  SRR4302533.1975633/1‑94 (MQ=60)
                               CATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGAT                                                               >  SRR4302533.3814090/1‑94 (MQ=60)
                                 TTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGA                                                             <  SRR4302533.5176553/94‑1 (MQ=60)
                                  TACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACATGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGAT                                                            <  SRR4302533.4458232/94‑1 (MQ=60)
                                  TACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGAT                                                            <  SRR4302533.3875037/94‑1 (MQ=60)
                                   ACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATG                                                           <  SRR4302533.1991625/94‑1 (MQ=60)
                                    CGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGT                                                          >  SRR4302533.2966422/1‑94 (MQ=60)
                                     GACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTT                                                         >  SRR4302533.6060522/1‑94 (MQ=60)
                                     GACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTT                                                         >  SRR4302533.4726166/1‑94 (MQ=60)
                                     GACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTT                                                         >  SRR4302533.1787939/1‑94 (MQ=60)
                                      ACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTC                                                        <  SRR4302533.3856521/94‑1 (MQ=60)
                                      ACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTC                                                        <  SRR4302533.1062144/94‑1 (MQ=60)
                                       CCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCA                                                       <  SRR4302533.5988434/94‑1 (MQ=60)
                                       CCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCA                                                       <  SRR4302533.640295/94‑1 (MQ=60)
                                       CCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACAAGGGAAAGATGAACGTGATGATGTTCA                                                       >  SRR4302533.3998397/1‑94 (MQ=60)
                                          GTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAA                                                    >  SRR4302533.5190267/1‑94 (MQ=60)
                                          GTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAA                                                    >  SRR4302533.3515729/1‑94 (MQ=60)
                                          GTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAA                                                    >  SRR4302533.1097303/1‑94 (MQ=60)
                                            CTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATT                                                  >  SRR4302533.19466/1‑94 (MQ=60)
                                             TAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTT                                                 >  SRR4302533.3518551/1‑94 (MQ=60)
                                             TAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTT                                                 >  SRR4302533.343813/1‑94 (MQ=60)
                                             TAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCAGTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTT                                                 <  SRR4302533.2435707/94‑1 (MQ=60)
                                              AAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTG                                                >  SRR4302533.3469857/1‑94 (MQ=60)
                                                AAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCT                                              <  SRR4302533.732963/94‑1 (MQ=60)
                                                 AAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTG                                             <  SRR4302533.5181812/94‑1 (MQ=60)
                                                 AAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTG                                             >  SRR4302533.5452224/1‑94 (MQ=60)
                                                 AAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTG                                             >  SRR4302533.2677166/1‑94 (MQ=60)
                                                    CGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAAT                                          >  SRR4302533.2337940/1‑94 (MQ=60)
                                                     GCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATT                                         <  SRR4302533.5241195/94‑1 (MQ=60)
                                                     GCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATT                                         <  SRR4302533.46728/94‑1 (MQ=60)
                                                      CCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTG                                        <  SRR4302533.5814055/94‑1 (MQ=60)
                                                      CCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTG                                        >  SRR4302533.3914357/1‑94 (MQ=60)
                                                      CCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTG                                        >  SRR4302533.3591112/1‑94 (MQ=60)
                                                       CTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGT                                       <  SRR4302533.1215826/94‑1 (MQ=60)
                                                       CTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGT                                       >  SRR4302533.2417488/1‑94 (MQ=60)
                                                       CTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGGTCACAATTTGCTGAATTGT                                       >  SRR4302533.2482471/1‑94 (MQ=60)
                                                        TGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTG                                      <  SRR4302533.372886/94‑1 (MQ=60)
                                                         GAATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGG                                     <  SRR4302533.616786/94‑1 (MQ=60)
                                                           ATTCGCGCCCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTG                                   <  SRR4302533.2333019/94‑1 (MQ=60)
                                                           ATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTG                                   <  SRR4302533.4510354/94‑1 (MQ=60)
                                                           ATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTG                                   <  SRR4302533.2714075/94‑1 (MQ=60)
                                                           ATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTG                                   >  SRR4302533.5947236/1‑94 (MQ=60)
                                                           ATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTG                                   <  SRR4302533.3360093/94‑1 (MQ=60)
                                                           ATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTG                                   <  SRR4302533.4239848/94‑1 (MQ=60)
                                                           ATTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTG                                   >  SRR4302533.4051325/1‑94 (MQ=60)
                                                            TTCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGA                                  <  SRR4302533.964835/94‑1 (MQ=60)
                                                             TCGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGAT                                 <  SRR4302533.4583838/94‑1 (MQ=60)
                                                              CGCGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATG                                <  SRR4302533.47873/94‑1 (MQ=60)
                                                                CGACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTG                              <  SRR4302533.3575690/94‑1 (MQ=60)
                                                                 GACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGA                             >  SRR4302533.1076791/1‑94 (MQ=60)
                                                                 GACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGA                             >  SRR4302533.3569188/1‑94 (MQ=60)
                                                                  GCCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGAT                            <  SRR4302533.834832/94‑1 (MQ=60)
                                                                  ACCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGAT                            <  SRR4302533.3448840/94‑1 (MQ=60)
                                                                   CCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATG                           <  SRR4302533.5584005/94‑1 (MQ=60)
                                                                   CCTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATG                           >  SRR4302533.3368288/1‑94 (MQ=60)
                                                                    CTTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGC                          >  SRR4302533.6162699/1‑94 (MQ=60)
                                                                     TTCTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCT                         >  SRR4302533.3771488/1‑94 (MQ=60)
                                                                       CTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCA                       <  SRR4302533.4078923/94‑1 (MQ=60)
                                                                       CTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCA                       >  SRR4302533.2196558/1‑94 (MQ=60)
                                                                       CTCGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCA                       >  SRR4302533.320978/1‑94 (MQ=60)
                                                                         CGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACC                     <  SRR4302533.4767249/94‑1 (MQ=60)
                                                                         CGTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACC                     <  SRR4302533.6198443/94‑1 (MQ=60)
                                                                          GTTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCG                    <  SRR4302533.2415744/94‑1 (MQ=60)
                                                                           TTACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGC                   <  SRR4302533.2332928/94‑1 (MQ=60)
                                                                            TACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCA                  >  SRR4302533.5612610/1‑94 (MQ=60)
                                                                             ACTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCAT                 <  SRR4302533.727680/94‑1 (MQ=60)
                                                                              CTGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATT                >  SRR4302533.6164272/1‑94 (MQ=60)
                                                                               TGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTT               >  SRR4302533.2737107/1‑94 (MQ=60)
                                                                               TGACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTT               >  SRR4302533.1395374/1‑94 (MQ=60)
                                                                                 ACAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCC             >  SRR4302533.2065672/1‑94 (MQ=60)
                                                                                  CAGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCT            >  SRR4302533.1366147/1‑94 (MQ=60)
                                                                                   AGGAAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTG           <  SRR4302533.3060407/94‑1 (MQ=60)
                                                                                      AAAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAA        >  SRR4302533.4749743/1‑94 (MQ=60)
                                                                                       AAATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAA       >  SRR4302533.4312645/1‑94 (MQ=60)
                                                                                        AATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAAT      >  SRR4302533.5571464/1‑94 (MQ=60)
                                                                                        AATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAAT      >  SRR4302533.5734610/1‑94 (MQ=60)
                                                                                         ATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATT     >  SRR4302533.3647353/1‑94 (MQ=60)
                                                                                         ATGAGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATT     <  SRR4302533.1483619/94‑1 (MQ=60)
                                                                                            AGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCAC  <  SRR4302533.6152504/94‑1 (MQ=60)
                                                                                            AGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCAC  >  SRR4302533.2657341/1‑94 (MQ=60)
                                                                                            |                                                                                             
CGAAAGGTCATAATGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCAC  >  NC_000913/4095019‑4095204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑