Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,128,799 T→C V462A (GTA→GCA)  metL → aspartokinase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,128,7990TC99.3% 962.1 / ‑4.1 300V462A (GTA→GCA) metLaspartokinase II
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (147/151);  total (148/152)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.07e-01

Only 100 of 310 total aligned reads displayed.

TGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGT  >  NC_000913/4128709‑4128888
                                                                                          |                                                                                         
tggtggCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAACCGCATCGGCCTGGCAt                                                                                         >  1:2688730/1‑93 (MQ=255)
tggtggCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCAt                                                                                         <  2:225221/93‑1 (MQ=255)
tggtggCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCAt                                                                                         >  2:2777034/1‑93 (MQ=255)
tggtggCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCAt                                                                                         >  1:4046814/1‑93 (MQ=255)
 ggtggCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGTGCAGAAAAACGCATCGGCCTGGCAtt                                                                                        >  1:9374585/1‑93 (MQ=255)
 ggtggCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCAtt                                                                                        >  1:7095288/1‑93 (MQ=255)
  gtggCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTg                                                                                       <  1:8376720/93‑1 (MQ=255)
  gtggCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTg                                                                                       >  1:9190273/1‑93 (MQ=255)
  gtggCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTg                                                                                       <  1:3213353/93‑1 (MQ=255)
   tggCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGt                                                                                      <  1:3637920/93‑1 (MQ=255)
   tggCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGt                                                                                      >  1:9532150/1‑93 (MQ=255)
   tggCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGt                                                                                      <  1:7353516/93‑1 (MQ=255)
    ggCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGtt                                                                                     >  2:5421758/1‑93 (MQ=255)
     gCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTc                                                                                    >  2:3953584/1‑93 (MQ=255)
     gCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTc                                                                                    <  1:1425170/93‑1 (MQ=255)
     gCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTc                                                                                    >  1:8792570/1‑93 (MQ=255)
     gCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTc                                                                                    <  2:6420296/93‑1 (MQ=255)
       aGTACTGCGCACCGGCCCGACCGTAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCgg                                                                                  <  2:5517134/93‑1 (MQ=255)
          aCTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTaa                                                                               <  2:4376437/93‑1 (MQ=255)
          aCTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTGCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTaa                                                                               <  2:9084722/93‑1 (MQ=255)
          aCTGCCCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTaa                                                                               <  2:4192484/93‑1 (MQ=255)
            tGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAgg                                                                             <  2:839750/93‑1 (MQ=255)
            tGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAgg                                                                             <  2:3406301/93‑1 (MQ=255)
            tGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAgg                                                                             >  1:6269837/1‑93 (MQ=255)
              cgcACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGc                                                                           <  1:2871806/93‑1 (MQ=255)
               gcACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCa                                                                          <  1:5990944/93‑1 (MQ=255)
                cACCGGGCCGACCGGAAGTCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCaa                                                                         <  2:1361349/93‑1 (MQ=255)
                cACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCaa                                                                         <  1:8284250/93‑1 (MQ=255)
                 aCCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAat                                                                        >  1:9106274/1‑93 (MQ=255)
                  ccGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAata                                                                       <  2:4662819/93‑1 (MQ=255)
                  ccGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAata                                                                       <  2:2502744/93‑1 (MQ=255)
                   cGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAatat                                                                      <  2:9830734/93‑1 (MQ=255)
                    ggCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATc                                                                     <  1:1942308/93‑1 (MQ=255)
                     gCCCGACCGAAAGCCTGATTCCGGAGGTGCGTCAATCCGTCTTCCTCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAAGGCAATATCg                                                                    >  2:5179742/1‑93 (MQ=255)
                     gCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCg                                                                    >  2:4040900/1‑93 (MQ=255)
                     gCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCg                                                                    >  1:4947826/1‑93 (MQ=255)
                      cccGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCgg                                                                   <  1:5003530/93‑1 (MQ=255)
                       ccgaccgaAAGCCTGATTCAGGGGCAGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCAGGCATTGTTCGGTAAGGGCAATATCGGt                                                                  >  1:4458768/1‑93 (MQ=255)
                         gaccgaAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTc                                                                <  1:4245360/93‑1 (MQ=255)
                            cgaAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCg                                                             >  2:1287466/1‑93 (MQ=255)
                             gaAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGCTCCCGt                                                            >  1:10026895/1‑93 (MQ=255)
                              aaaGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGtt                                                           >  2:9596168/1‑93 (MQ=255)
                              aaaGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGtt                                                           <  1:1106521/93‑1 (MQ=255)
                              aaaGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGtt                                                           <  2:1210219/93‑1 (MQ=255)
                              aaaGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGtt                                                           <  1:1652998/93‑1 (MQ=255)
                               aaGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGGCTGGCATTGTTCGGGAAGGGCAATATCGGTTTCCGTTg                                                          >  2:6774695/1‑93 (MQ=255)
                                aGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTgg                                                         >  1:2205406/1‑93 (MQ=255)
                                    tGATTAAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTgg                                                     <  1:4751311/93‑1 (MQ=255)
                                      aTTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGaa                                                   >  2:7709074/1‑93 (MQ=255)
                                        tCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACt                                                 >  2:2115782/1‑93 (MQ=255)
                                          aGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGt                                               <  2:872765/93‑1 (MQ=255)
                                          aGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGt                                               <  2:7011885/93‑1 (MQ=255)
                                           ggggCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGtt                                              >  2:5888674/1‑93 (MQ=255)
                                           ggggCTGCATCAGTCCGCCTTCCGCGCAGAAAACCGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGtt                                              <  1:2007562/93‑1 (MQ=255)
                                            gggCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGGTc                                             >  2:2989150/1‑93 (MQ=255)
                                             ggCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCg                                            <  2:9353533/93‑1 (MQ=255)
                                                tGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGccc                                         >  2:1112653/1‑93 (MQ=255)
                                                tGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGccc                                         >  1:2326792/1‑93 (MQ=255)
                                                 gCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCg                                        <  2:7343604/93‑1 (MQ=255)
                                                 gCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCg                                        >  1:984261/1‑93 (MQ=255)
                                                   aTCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTg                                      >  2:885571/1‑93 (MQ=255)
                                                   aTCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTg                                      >  2:712271/1‑93 (MQ=255)
                                                   aTCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTg                                      >  2:1129822/1‑93 (MQ=255)
                                                   aTCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCCGGAACTGGTAGCCCGTg                                      >  2:6133297/1‑93 (MQ=255)
                                                    tCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTGCGGTTGGCTGGAACTGTTCGCACGTgg                                     >  1:2399977/1‑92 (MQ=255)
                                                      aGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagc                                   >  2:2359088/1‑93 (MQ=255)
                                                      aGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagc                                   >  2:5046088/1‑93 (MQ=255)
                                                         ccGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagcaga                                >  2:2941920/1‑93 (MQ=255)
                                                         ccGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagcaga                                <  2:7737265/93‑1 (MQ=255)
                                                          cGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagcagag                               >  1:5363481/1‑93 (MQ=255)
                                                          cGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagcagag                               <  1:6773851/93‑1 (MQ=255)
                                                           gTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagcagagc                              <  1:9616132/93‑1 (MQ=255)
                                                           gTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagcagagc                              <  2:4789948/93‑1 (MQ=255)
                                                           gTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagcagagc                              >  2:1710944/1‑93 (MQ=255)
                                                            tCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagcagagca                             >  1:7805690/1‑93 (MQ=255)
                                                            tCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagcagagca                             <  2:2603762/93‑1 (MQ=255)
                                                              ttCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACg                           >  2:7968904/1‑93 (MQ=255)
                                                              ttCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACg                           >  2:6878321/1‑93 (MQ=255)
                                                                ccGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCt                         >  2:9011152/1‑93 (MQ=255)
                                                                ccGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCt                         <  2:4597101/93‑1 (MQ=255)
                                                                  gcgcAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCttt                       <  1:3972005/93‑1 (MQ=255)
                                                                   cgcAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTc                      <  1:5700753/93‑1 (MQ=255)
                                                                   cgcAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTc                      >  2:5640855/1‑93 (MQ=255)
                                                                    gcAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCg                     <  2:8103744/93‑1 (MQ=255)
                                                                       gAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCa                  <  2:359811/93‑1 (MQ=255)
                                                                       gAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCa                  >  2:1920465/1‑93 (MQ=255)
                                                                        aaaaaCGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCAc                 <  2:6505475/93‑1 (MQ=255)
                                                                        aaaaaCGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCAc                 <  2:9674637/93‑1 (MQ=255)
                                                                            aCGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTAc             >  1:9893402/1‑93 (MQ=255)
                                                                             cGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGAAca            <  2:3474845/93‑2 (MQ=255)
                                                                               cATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTATCgg          >  2:1794771/1‑93 (MQ=255)
                                                                               cATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCgg          <  1:8201854/93‑1 (MQ=255)
                                                                                 tCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGATCACGCTTTCGGCACGTACCGGCt        >  2:7231307/1‑93 (MQ=255)
                                                                                 tCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCt        >  1:5596546/1‑93 (MQ=255)
                                                                                   ggCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCttt      <  1:833995/93‑1 (MQ=255)
                                                                                    gCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTg     >  2:9560606/1‑93 (MQ=255)
                                                                                     ccTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGa    <  2:5991568/93‑1 (MQ=255)
                                                                                      cTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAg   >  2:6697865/1‑93 (MQ=255)
                                                                                       tGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGCGCAGGATTTCGGCACGTACCGGCTTTGAGt  >  1:8587070/1‑93 (MQ=255)
                                                                                       tGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACAGGCTTTGAGt  >  1:6325844/1‑93 (MQ=255)
                                                                                          |                                                                                         
TGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGT  >  NC_000913/4128709‑4128888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGT  >  NC_000913/4128706‑4128892
                                                                                             |                                                                                             
GCCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGC                                                                                               >  SRR4302540.8916236/1‑94 (MQ=60)
 CCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCA                                                                                              <  SRR4302540.713010/94‑1 (MQ=60)
  cctgtgggcgtaccgcgccCCGGCCCCACCGAAAACCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCAT                                                                                             <  SRR4302540.7700308/75‑1 (MQ=60)
  CTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAACCGCATCGGCCTGGCAT                                                                                             >  SRR4302540.2717785/1‑94 (MQ=60)
  CTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCAT                                                                                             <  SRR4302540.4025886/94‑1 (MQ=60)
  CTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCAT                                                                                             >  SRR4302540.4090450/1‑94 (MQ=60)
   TGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGTGCAGAAAAACGCATCGGCCTGGCATT                                                                                            >  SRR4302540.9486184/1‑94 (MQ=60)
   TGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATT                                                                                            >  SRR4302540.10019665/1‑94 (MQ=60)
   TGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATT                                                                                            >  SRR4302540.7174248/1‑94 (MQ=60)
   TGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATT                                                                                            <  SRR4302540.1131937/94‑1 (MQ=60)
    GGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTG                                                                                           >  SRR4302540.9300493/1‑94 (MQ=60)
     GTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGT                                                                                          <  SRR4302540.8473923/94‑1 (MQ=60)
     GTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGT                                                                                          <  SRR4302540.3248239/94‑1 (MQ=60)
     GTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGT                                                                                          >  SRR4302540.4664494/1‑94 (MQ=60)
     GTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGT                                                                                          >  SRR4302540.9644995/1‑94 (MQ=60)
     GTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGT                                                                                          <  SRR4302540.4028396/94‑1 (MQ=60)
      TGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTT                                                                                         <  SRR4302540.2914040/94‑1 (MQ=60)
      TGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTT                                                                                         >  SRR4302540.2516401/1‑94 (MQ=60)
      TGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTT                                                                                         <  SRR4302540.3677750/94‑1 (MQ=60)
      TGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTT                                                                                         <  SRR4302540.7435536/94‑1 (MQ=60)
       GGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTC                                                                                        <  SRR4302540.8449384/94‑1 (MQ=60)
       GGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTC                                                                                        >  SRR4302540.8894277/1‑94 (MQ=60)
        GCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCG                                                                                       <  SRR4302540.5490973/94‑1 (MQ=60)
        GCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCG                                                                                       <  SRR4302540.1440596/94‑1 (MQ=60)
         CAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGG                                                                                      <  SRR4302540.3083972/94‑1 (MQ=60)
          AGTACTGCGCACCGGCCCGACCGAAAGGCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGT                                                                                     >  SRR4302540.2689414/1‑94 (MQ=60)
          AGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGT                                                                                     >  SRR4302540.2325613/1‑94 (MQ=60)
          AGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGT                                                                                     >  SRR4302540.9464319/1‑94 (MQ=60)
             ACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAG                                                                                  <  SRR4302540.849169/94‑1 (MQ=60)
             ACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAG                                                                                  <  SRR4302540.4598571/94‑1 (MQ=60)
             ACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAG                                                                                  >  SRR4302540.9709213/1‑94 (MQ=60)
             ACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAG                                                                                  >  SRR4302540.2764730/1‑94 (MQ=60)
             ACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAG                                                                                  <  SRR4302540.6747412/94‑1 (MQ=60)
             ACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAG                                                                                  <  SRR4302540.723526/94‑1 (MQ=60)
              CTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGG                                                                                 >  SRR4302540.6339596/1‑94 (MQ=60)
                 CGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCA                                                                              <  SRR4302540.2903013/94‑1 (MQ=60)
                 CGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCA                                                                              >  SRR4302540.9477410/1‑94 (MQ=60)
                 CGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCA                                                                              <  SRR4302540.1500021/94‑1 (MQ=60)
                  GCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAA                                                                             <  SRR4302540.6057316/94‑1 (MQ=60)
                   CACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAAT                                                                            <  SRR4302540.7549918/94‑1 (MQ=60)
                   CACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAAT                                                                            <  SRR4302540.8380471/94‑1 (MQ=60)
                   CACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAAT                                                                            >  SRR4302540.9215706/1‑94 (MQ=60)
                   CACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAAT                                                                            <  SRR4302540.7167689/94‑1 (MQ=60)
                    ACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATA                                                                           >  SRR4302540.8431146/1‑94 (MQ=60)
                    ACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATA                                                                           <  SRR4302540.5198605/94‑1 (MQ=60)
                       GGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCG                                                                        <  SRR4302540.6172224/94‑1 (MQ=60)
                       GGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCG                                                                        >  SRR4302540.5001259/1‑94 (MQ=60)
                       GGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCG                                                                        <  SRR4302540.8954020/94‑1 (MQ=60)
                       GGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCG                                                                        <  SRR4302540.1963457/94‑1 (MQ=60)
                         CCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGT                                                                      <  SRR4302540.5057717/94‑1 (MQ=60)
                         CCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGT                                                                      <  SRR4302540.5057718/94‑1 (MQ=60)
                         CCCGACCGAAAGCCTGATTCAGGGGCAGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCAGGCATTGTTCGGTAAGGGCAATATCGGT                                                                      >  SRR4302540.4507101/1‑94 (MQ=60)
                          CCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTT                                                                     >  SRR4302540.8375053/1‑94 (MQ=60)
                          CCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTT                                                                     >  SRR4302540.4361620/1‑94 (MQ=60)
                            GACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCC                                                                   <  SRR4302540.4291546/94‑1 (MQ=60)
                            GACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCC                                                                   >  SRR4302540.5004365/1‑94 (MQ=60)
                              CCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCG                                                                 <  SRR4302540.2844957/94‑1 (MQ=60)
                              CCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCG                                                                 <  SRR4302540.5991904/94‑1 (MQ=60)
                              ACGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCG                                                                 <  SRR4302540.2056290/94‑1 (MQ=60)
                               CGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGCTCCCGT                                                                >  SRR4302540.10143561/1‑94 (MQ=60)
                                GAAATCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTT                                                               <  SRR4302540.3027595/94‑1 (MQ=60)
                                GAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTT                                                               >  SRR4302540.1098285/1‑94 (MQ=60)
                                GAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTT                                                               >  SRR4302540.4195594/1‑94 (MQ=60)
                                 AAATCCTGATTCAGGGGTTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGGTCCCGTTG                                                              <  SRR4302540.6497481/94‑1 (MQ=60)
                                 AAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTG                                                              <  SRR4302540.1671018/94‑1 (MQ=60)
                                 AAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTG                                                              <  SRR4302540.1117942/94‑1 (MQ=60)
                                 AAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTG                                                              <  SRR4302540.4376713/94‑1 (MQ=60)
                                 AAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTG                                                              <  SRR4302540.1671017/94‑1 (MQ=60)
                                  AAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGG                                                             <  SRR4302540.6936336/94‑1 (MQ=60)
                                  AAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGG                                                             >  SRR4302540.2229234/1‑94 (MQ=60)
                                     CCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTG                                                          <  SRR4302540.9244000/94‑1 (MQ=60)
                                      CTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGG                                                         >  SRR4302540.9569941/1‑94 (MQ=60)
                                       TGATTAAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGA                                                        <  SRR4302540.4802014/94‑1 (MQ=60)
                                        GATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCCTCGGACTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAA                                                       >  SRR4302540.6015285/1‑94 (MQ=60)
                                          TTCAGGGGCTACATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACT                                                     <  SRR4302540.8402172/94‑1 (MQ=60)
                                            CAGGGGGTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGT                                                   <  SRR4302540.2111336/94‑1 (MQ=60)
                                              GGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTC                                                 <  SRR4302540.9844501/94‑1 (MQ=60)
                                              GGGGCTGCATCAGTCCGCCTTCCGCGCAGAAAACCGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTC                                                 <  SRR4302540.2029280/94‑1 (MQ=60)
                                               GGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCG                                                >  SRR4302540.1629491/1‑94 (MQ=60)
                                                 GCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCC                                              <  SRR4302540.4873468/94‑1 (MQ=60)
                                                  CTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCC                                             >  SRR4302540.2351851/1‑94 (MQ=60)
                                                   TGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCG                                            >  SRR4302540.994327/1‑94 (MQ=60)
                                                   TGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCG                                            >  SRR4302540.4051452/1‑94 (MQ=60)
                                                    GCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGT                                           >  SRR4302540.885760/1‑94 (MQ=60)
                                                      ATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTGCGGTTGGCTGGAACTGTTCGCACGTGG                                         >  SRR4302540.2425854/1‑94 (MQ=60)
                                                      ATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGA                                         <  SRR4302540.8437493/94‑1 (MQ=60)
                                                      ATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGA                                         >  SRR4302540.2425853/1‑94 (MQ=60)
                                                      ATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGA                                         >  SRR4302540.9978761/1‑94 (MQ=60)
                                                      ATCAATCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGA                                         <  SRR4302540.2460063/94‑1 (MQ=60)
                                                       TCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAG                                        <  SRR4302540.2285107/94‑1 (MQ=60)
                                                          GTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAG                                     >  SRR4302540.7212800/1‑94 (MQ=60)
                                                          GTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGGTCGCCCGTGAGCAG                                     >  SRR4302540.5760526/1‑94 (MQ=60)
                                                           TCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGA                                    >  SRR4302540.5688858/1‑94 (MQ=60)
                                                            CCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAG                                   >  SRR4302540.5422395/1‑94 (MQ=60)
                                                             CGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGC                                  <  SRR4302540.6848716/94‑1 (MQ=60)
                                                             CGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGC                                  >  SRR4302540.3507335/1‑94 (MQ=60)
                                                              GTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCA                                 <  SRR4302540.9729502/94‑1 (MQ=60)
                                                              GTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCA                                 >  SRR4302540.7896908/1‑94 (MQ=60)
                                                              GTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCA                                 >  SRR4302540.7953526/1‑94 (MQ=60)
                                                              GTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGCAAGAGCAATATCGGTTCCAGTTGGCTGGCACTGTTCGCCCGTGAGCAGAGCA                                 >  SRR4302540.5086436/1‑94 (MQ=60)
                                                               TCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCAC                                <  SRR4302540.6409702/94‑1 (MQ=60)
                                                               TCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCAC                                <  SRR4302540.237076/94‑1 (MQ=60)
                                                               TCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCAC                                <  SRR4302540.989847/94‑1 (MQ=60)
                                                               TCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCAC                                <  SRR4302540.1791997/94‑1 (MQ=60)
                                                               TCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCAC                                >  SRR4302540.5497678/1‑94 (MQ=60)
                                                               TCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCAC                                <  SRR4302540.6383996/94‑1 (MQ=60)
                                                                 TTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGC                              <  SRR4302540.4812440/94‑1 (MQ=60)
                                                                  TCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCT                             >  SRR4302540.4240330/1‑94 (MQ=60)
                                                                    CGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTT                           <  SRR4302540.9517420/94‑1 (MQ=60)
                                                                     GCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTC                          <  SRR4302540.1063018/94‑1 (MQ=60)
                                                                     GCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTC                          <  SRR4302540.4014818/94‑1 (MQ=60)
                                                                     GCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACCGTTCGCCCGTGAGCAGAGCACGCTTTC                          <  SRR4302540.197440/94‑1 (MQ=60)
                                                                      CGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCG                         >  SRR4302540.8308333/1‑94 (MQ=60)
                                                                      CGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCG                         <  SRR4302540.38893/94‑1 (MQ=60)
                                                                      CGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCG                         >  SRR4302540.9011654/1‑94 (MQ=60)
                                                                      CGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCG                         <  SRR4302540.5763515/94‑1 (MQ=60)
                                                                        CAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGC                       >  SRR4302540.8217565/1‑94 (MQ=60)
                                                                        CAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCATTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGC                       <  SRR4302540.9697694/94‑1 (MQ=60)
                                                                           AAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACG                    >  SRR4302540.5110223/1‑94 (MQ=60)
                                                                            AAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGT                   >  SRR4302540.7910932/1‑94 (MQ=60)
                                                                             AAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTA                  >  SRR4302540.771576/1‑94 (MQ=60)
                                                                              AACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTAC                 >  SRR4302540.10008933/1‑94 (MQ=60)
                                                                              AACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTAC                 >  SRR4302540.1330655/1‑94 (MQ=60)
                                                                                CGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCG               <  SRR4302540.8486218/94‑1 (MQ=60)
                                                                                CGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCG               <  SRR4302540.9703785/94‑1 (MQ=60)
                                                                                CGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCG               >  SRR4302540.3755515/1‑94 (MQ=60)
                                                                                CGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCG               <  SRR4302540.1976281/94‑1 (MQ=60)
                                                                                  CATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGC             <  SRR4302540.8297164/94‑1 (MQ=60)
                                                                                  CATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGC             <  SRR4302540.9967254/94‑1 (MQ=60)
                                                                                   ATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCT            >  SRR4302540.5658117/1‑94 (MQ=60)
                                                                                   ATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTA‑CGGCTT           >  SRR4302540.327848/1‑94 (MQ=60)
                                                                                      GGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTG         <  SRR4302540.6328224/94‑1 (MQ=60)
                                                                                      GGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTG         >  SRR4302540.9257421/1‑94 (MQ=60)
                                                                                      GGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTG         <  SRR4302540.842852/94‑1 (MQ=60)
                                                                                       GCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGA        <  SRR4302540.8720016/94‑1 (MQ=60)
                                                                                         CTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGCGCAGGATTTCGGCACGTACCGGCTTTGAGT      >  SRR4302540.8686893/1‑94 (MQ=60)
                                                                                         CTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGT      >  SRR4302540.6395985/1‑94 (MQ=60)
                                                                                         CTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGT      <  SRR4302540.2807045/94‑1 (MQ=60)
                                                                                         CTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACAGGCTTTGAGT      >  SRR4302540.6396288/1‑94 (MQ=60)
                                                                                            GCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCCCGTACCGGCTTTGAGTTTG   <  SRR4302540.1738146/94‑1 (MQ=60)
                                                                                            GCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTG   <  SRR4302540.10153116/94‑1 (MQ=60)
                                                                                            GCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTG   <  SRR4302540.7657691/94‑1 (MQ=60)
                                                                                             CATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGT  <  SRR4302540.1651063/94‑1 (MQ=60)
                                                                                             |                                                                                             
GCCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGT  >  NC_000913/4128706‑4128892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑