Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,128,799 T→C V462A (GTA→GCA)  metL → aspartokinase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,128,7990TC99.6% 851.7 / ‑6.6 265V462A (GTA→GCA) metLaspartokinase II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (141/123);  minor base A (0/1);  total (141/124)
Fisher's exact test for biased strand distribution p-value = 4.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 270 total aligned reads displayed.

TGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGT  >  NC_000913/4128709‑4128888
                                                                                          |                                                                                         
 ggtggCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCAtt                                                                                        >  2:499396/1‑93 (MQ=255)
 ggtggCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCAtt                                                                                        >  1:5051410/1‑93 (MQ=255)
  gtggCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTg                                                                                       <  1:5769849/93‑1 (MQ=255)
   tggCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGt                                                                                      >  2:1099889/1‑93 (MQ=255)
   tggCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGt                                                                                      <  2:2444629/93‑1 (MQ=255)
    ggCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGtt                                                                                     <  2:259318/93‑1 (MQ=255)
      cAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCg                                                                                   >  2:5880510/1‑93 (MQ=255)
      cAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCg                                                                                   <  1:3988331/93‑1 (MQ=255)
       aGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCgg                                                                                  >  1:4425833/1‑93 (MQ=255)
       aGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCgg                                                                                  <  2:5435362/93‑1 (MQ=255)
        gTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGt                                                                                 >  1:3738824/1‑93 (MQ=255)
         tACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTa                                                                                >  2:633992/1‑93 (MQ=255)
          aCTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTaa                                                                               <  1:4266898/93‑1 (MQ=255)
            tGCGCGCCGCCCACACCGAAAGCCTGATTGAGTGGCTGCATAAGTCCGTCTGCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAgg                                                                             <  1:2179400/93‑1 (MQ=255)
              cgcACCGCCCCGTCCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGTCATTGTTCGGTAAGGGc                                                                           <  2:1034528/93‑1 (MQ=255)
               gcACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTCAGGGCa                                                                          >  2:3218443/1‑93 (MQ=255)
               gcACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCa                                                                          <  1:4151843/93‑1 (MQ=255)
               gcACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTATTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCa                                                                          >  1:4987530/1‑93 (MQ=255)
                cACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCaa                                                                         <  1:5307641/93‑1 (MQ=255)
                 aCCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAat                                                                        <  2:3377537/93‑1 (MQ=255)
                  ccGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAata                                                                       >  1:3168480/1‑93 (MQ=255)
                  ccGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAata                                                                       <  2:4849493/93‑1 (MQ=255)
                    ggCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATc                                                                     <  1:1488096/93‑1 (MQ=255)
                    ggCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATc                                                                     >  1:4490074/1‑93 (MQ=255)
                    cgcccgcccGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATc                                                                     <  1:1640286/92‑1 (MQ=255)
                     gCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCg                                                                    <  1:4031126/93‑1 (MQ=255)
                     gCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCg                                                                    <  2:1610285/93‑1 (MQ=255)
                     gCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCg                                                                    >  2:2312457/1‑93 (MQ=255)
                      cccGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCgg                                                                   <  1:6231419/93‑1 (MQ=255)
                      cccGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCgg                                                                   >  1:5633458/1‑93 (MQ=255)
                       ccgaccgaAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGt                                                                  >  1:1885448/1‑93 (MQ=255)
                       ccgaccgaAAGCCTGATTCAGGGGCTGCATCAGTCCGACTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGt                                                                  <  1:1155382/93‑1 (MQ=255)
                        cgaccgaAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGtt                                                                 >  2:2137823/1‑93 (MQ=255)
                         gaccgaAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTc                                                                <  2:5149322/93‑1 (MQ=255)
                         gaccgaAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTc                                                                >  2:4422858/1‑93 (MQ=255)
                          accgaAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTcc                                                               >  1:3629639/1‑93 (MQ=255)
                          accgaAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTcc                                                               >  1:1696609/1‑93 (MQ=255)
                           ccgaAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTccc                                                              >  1:467068/1‑93 (MQ=255)
                            cgaAAGCCTGATTCAGGGGCTGCACCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCg                                                             >  1:769034/1‑93 (MQ=255)
                             gaAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCGGAAAAACGCATCGGCCTGGCATTGTTCGGTTAGGGCAATATTGGTTcccct                                                            >  1:71577/1‑91 (MQ=255)
                             gaAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGt                                                            >  2:4287135/1‑93 (MQ=255)
                               aaGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTg                                                          <  1:5571895/93‑1 (MQ=255)
                               aaGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTg                                                          <  1:5890323/93‑1 (MQ=255)
                                 gCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGc                                                        >  2:2918954/1‑93 (MQ=255)
                                 gCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGc                                                        >  2:5335610/1‑93 (MQ=255)
                                  ccTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCt                                                       >  2:5842089/1‑93 (MQ=255)
                                   cTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTg                                                      >  1:1316864/1‑93 (MQ=255)
                                   cTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTg                                                      >  1:4940411/1‑93 (MQ=255)
                                      aTTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGaa                                                   <  2:1462740/93‑1 (MQ=255)
                                       ttCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAAc                                                  >  2:6218599/1‑93 (MQ=255)
                                       ttCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAAc                                                  >  2:385772/1‑93 (MQ=255)
                                       ttCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAAc                                                  >  2:1240086/1‑93 (MQ=255)
                                        tCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACt                                                 >  2:458664/1‑93 (MQ=255)
                                         cAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTg                                                >  2:2227258/1‑93 (MQ=255)
                                          aGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTGCCGTTGGCTGGAACTGt                                               >  2:6288825/1‑93 (MQ=255)
                                          aGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGt                                               <  2:3645957/93‑1 (MQ=255)
                                           ggggCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGtt                                              <  2:4190007/93‑1 (MQ=255)
                                           ggggCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGtt                                              <  1:2330746/93‑1 (MQ=255)
                                           ggggCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGtt                                              <  1:2843185/93‑1 (MQ=255)
                                           ggggCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGtt                                              <  2:3924908/93‑1 (MQ=255)
                                            gggCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTc                                             <  2:2110557/93‑1 (MQ=255)
                                             ggCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCg                                            >  1:2279507/1‑93 (MQ=255)
                                               cTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGcc                                          <  2:3163969/93‑1 (MQ=255)
                                               cTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGcc                                          <  2:6135909/93‑1 (MQ=255)
                                               cTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGcc                                          <  2:4050207/93‑1 (MQ=255)
                                                 gCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCg                                        <  2:1768673/93‑1 (MQ=255)
                                                 gCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGCTCGCCCg                                        >  1:2719854/1‑93 (MQ=255)
                                                  cATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGt                                       <  1:601816/93‑1 (MQ=255)
                                                   aTCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTg                                      >  2:5682366/1‑93 (MQ=255)
                                                    tCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTga                                     >  1:5436872/1‑93 (MQ=255)
                                                      aGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagc                                   <  2:3546511/93‑1 (MQ=255)
                                                         ccGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagcaga                                <  2:1973108/93‑1 (MQ=255)
                                                         ccGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagcaga                                <  1:1709054/93‑1 (MQ=255)
                                                          cGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagcagag                               >  1:721065/1‑93 (MQ=255)
                                                           gTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagcagagc                              >  2:760987/1‑93 (MQ=255)
                                                             cTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCAc                            <  1:4568730/93‑1 (MQ=255)
                                                             cTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCAc                            <  1:5207490/93‑1 (MQ=255)
                                                                  gcgcAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCttt                       >  1:2044733/1‑93 (MQ=255)
                                                                  gcgcAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCttt                       <  1:3363325/93‑1 (MQ=255)
                                                                    gcAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCg                     <  2:2889001/93‑1 (MQ=255)
                                                                     cAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCgg                    <  2:3470037/93‑1 (MQ=255)
                                                                      aGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGc                   >  2:1551443/1‑93 (MQ=255)
                                                                       gAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCa                  >  2:3787588/1‑93 (MQ=255)
                                                                       gAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCa                  >  2:4549772/1‑93 (MQ=255)
                                                                        aaaacCGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCAc                 <  1:794704/93‑1 (MQ=255)
                                                                         aaaaCGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACg                >  1:2213053/1‑93 (MQ=255)
                                                                           aaCGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTa              <  1:647523/93‑1 (MQ=255)
                                                                            aCGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTAc             >  1:1039863/1‑93 (MQ=255)
                                                                             cGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTAcc            <  1:2944276/93‑1 (MQ=255)
                                                                               cATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCTGCACGTACCgg          <  1:3164784/93‑1 (MQ=255)
                                                                                 tCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCt        >  2:3087193/1‑93 (MQ=255)
                                                                                  cGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCtt       <  1:1537190/93‑1 (MQ=255)
                                                                                   ggCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTCCCGGCttt      >  1:2126631/1‑93 (MQ=255)
                                                                                    gCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTg     <  1:241662/93‑1 (MQ=255)
                                                                                    gCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTg     >  2:1722205/1‑93 (MQ=255)
                                                                                     ccTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGa    <  2:4691087/93‑1 (MQ=255)
                                                                                      cTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAg   >  2:5049546/1‑93 (MQ=255)
                                                                                       tGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGt  <  1:2596182/93‑1 (MQ=255)
                                                                                       tGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGt  >  2:2700755/1‑93 (MQ=255)
                                                                                       tGGCATTGTTCGGGAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGt  >  2:3186512/1‑93 (MQ=255)
                                                                                          |                                                                                         
TGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGT  >  NC_000913/4128709‑4128888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTG  >  NC_000913/4128707‑4128891
                                                                                            |                                                                                            
CCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCA                                                                                             >  SRR4302533.5689027/1‑94 (MQ=60)
CCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCA                                                                                             >  SRR4302533.1664/1‑94 (MQ=60)
CCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCA                                                                                             >  SRR4302533.649461/1‑94 (MQ=60)
 CTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCAT                                                                                            >  SRR4302533.2700153/1‑94 (MQ=60)
  TGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATT                                                                                           >  SRR4302533.5081956/1‑94 (MQ=60)
   GGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTG                                                                                          <  SRR4302533.775885/94‑1 (MQ=60)
    GTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGT                                                                                         >  SRR4302533.931736/1‑94 (MQ=60)
    GTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGT                                                                                         <  SRR4302533.5804686/94‑1 (MQ=60)
      GGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTC                                                                                       >  SRR4302533.4283720/1‑94 (MQ=60)
        CAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGG                                                                                     <  SRR4302533.5613712/94‑1 (MQ=60)
        CAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGG                                                                                     >  SRR4302533.4072649/1‑94 (MQ=60)
        CAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGG                                                                                     <  SRR4302533.4012625/94‑1 (MQ=60)
        CAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGG                                                                                     >  SRR4302533.4452584/1‑94 (MQ=60)
         AGTACTGCTCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCTTGGCATTGTTCGGT                                                                                    <  SRR4302533.224428/94‑1 (MQ=60)
         AGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGT                                                                                    >  SRR4302533.5385724/1‑94 (MQ=60)
         AGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGT                                                                                    >  SRR4302533.3761754/1‑94 (MQ=60)
         AGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGT                                                                                    >  SRR4302533.1163025/1‑94 (MQ=60)
          GTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTA                                                                                   >  SRR4302533.3341570/1‑94 (MQ=60)
          GTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTA                                                                                   <  SRR4302533.2102059/94‑1 (MQ=60)
           TACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAA                                                                                  <  SRR4302533.5107250/94‑1 (MQ=60)
            ACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAG                                                                                 <  SRR4302533.4292593/94‑1 (MQ=60)
             CTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGG                                                                                >  SRR4302533.4670508/1‑94 (MQ=60)
             CTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGG                                                                                >  SRR4302533.584263/1‑94 (MQ=60)
              tgcgcgccgcccacACCGAAAGCCTGATTGAGTGGCTGCATAAGTCCGTCTGCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGG                                                                               <  SRR4302533.2192837/80‑1 (MQ=60)
              TGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGG                                                                               <  SRR4302533.1900568/94‑1 (MQ=60)
              TGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGG                                                                               <  SRR4302533.640507/94‑1 (MQ=60)
              TGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGG                                                                               <  SRR4302533.5475106/94‑1 (MQ=60)
              CGCGCTCCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCACAGAAAACCGCATCGGCCTGGCATTGTTCGGTAAGGG                                                                               <  SRR4302533.5889052/94‑1 (MQ=60)
                CGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTATTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCA                                                                             >  SRR4302533.5017770/1‑94 (MQ=60)
                 GCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAA                                                                            <  SRR4302533.4176999/94‑1 (MQ=60)
                  CACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAAT                                                                           <  SRR4302533.5339452/94‑1 (MQ=60)
                   ACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATA                                                                          >  SRR4302533.3187979/1‑94 (MQ=60)
                     CGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATC                                                                        >  SRR4302533.4517264/1‑94 (MQ=60)
                      GGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCG                                                                       <  SRR4302533.2343388/94‑1 (MQ=60)
                      GGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCG                                                                       <  SRR4302533.1497073/94‑1 (MQ=60)
                      CGCCCGCCCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCG                                                                       <  SRR4302533.1650221/94‑1 (MQ=60)
                       GCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGG                                                                      >  SRR4302533.5667524/1‑94 (MQ=60)
                       GCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGG                                                                      <  SRR4302533.2737104/94‑1 (MQ=60)
                       GCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGG                                                                      <  SRR4302533.4055665/94‑1 (MQ=60)
                        CCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGT                                                                     <  SRR4302533.6268737/94‑1 (MQ=60)
                        CCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGT                                                                     >  SRR4302533.1896964/1‑94 (MQ=60)
                         CCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTT                                                                    <  SRR4302533.634606/94‑1 (MQ=60)
                         CCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTT                                                                    <  SRR4302533.793487/94‑1 (MQ=60)
                         CCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGACTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTT                                                                    <  SRR4302533.1162312/94‑1 (MQ=60)
                          CGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTC                                                                   >  SRR4302533.1403695/1‑94 (MQ=60)
                           GACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCC                                                                  >  SRR4302533.1706884/1‑94 (MQ=60)
                           GACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCC                                                                  >  SRR4302533.3651972/1‑94 (MQ=60)
                            ACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCC                                                                 >  SRR4302533.469857/1‑94 (MQ=60)
                            ACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCC                                                                 <  SRR4302533.1296220/94‑1 (MQ=60)
                             CCGAAAGCCTGATTCAGGGGCTGCACCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCG                                                                >  SRR4302533.773565/1‑94 (MQ=60)
                              CGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCGGAAAAACGCATCGGCCTGGCATTGTTCGGTTAGGGCAATATTGGTTCCCCT                                                               >  SRR4302533.72037/1‑94 (MQ=60)
                              CGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGT                                                               >  SRR4302533.3753532/1‑94 (MQ=60)
                              CGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGT                                                               >  SRR4302533.1883422/1‑94 (MQ=60)
                               GAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTT                                                              <  SRR4302533.1778423/94‑1 (MQ=60)
                                 AAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGG                                                            <  SRR4302533.4714227/94‑1 (MQ=60)
                                 AAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGG                                                            <  SRR4302533.5605569/94‑1 (MQ=60)
                                 AAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGG                                                            <  SRR4302533.5925906/94‑1 (MQ=60)
                                 AAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGG                                                            <  SRR4302533.2372397/94‑1 (MQ=60)
                                 AAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGG                                                            >  SRR4302533.694080/1‑94 (MQ=60)
                                  AGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGC                                                           <  SRR4302533.4477074/94‑1 (MQ=60)
                                    CCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTG                                                         >  SRR4302533.4970319/1‑94 (MQ=60)
                                    CCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTG                                                         >  SRR4302533.1324765/1‑94 (MQ=60)
                                     CTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGG                                                        >  SRR4302533.659030/1‑94 (MQ=60)
                                      TGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGA                                                       >  SRR4302533.3598720/1‑94 (MQ=60)
                                      TGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGA                                                       >  SRR4302533.2929184/1‑94 (MQ=60)
                                      TGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGAAAGGGCAATATCGGCTCCCGTTGGCTGGA                                                       >  SRR4302533.2595737/1‑94 (MQ=60)
                                        ATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAAC                                                     >  SRR4302533.2989748/1‑94 (MQ=60)
                                        ATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAAC                                                     >  SRR4302533.2119743/1‑94 (MQ=60)
                                          TCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTG                                                   <  SRR4302533.2951501/94‑1 (MQ=60)
                                           CAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGT                                                  <  SRR4302533.5634868/94‑1 (MQ=60)
                                            AGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTT                                                 >  SRR4302533.1527190/1‑94 (MQ=60)
                                            AGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTT                                                 >  SRR4302533.2579406/1‑94 (MQ=60)
                                             GGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTC                                                <  SRR4302533.2860682/94‑1 (MQ=60)
                                             GGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTC                                                <  SRR4302533.2345177/94‑1 (MQ=60)
                                             GGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTC                                                <  SRR4302533.507769/94‑1 (MQ=60)
                                              GGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCG                                               <  SRR4302533.2858655/94‑1 (MQ=60)
                                              GGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCG                                               <  SRR4302533.3826464/94‑1 (MQ=60)
                                              GGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCG                                               <  SRR4302533.4550532/94‑1 (MQ=60)
                                              GGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCG                                               >  SRR4302533.2293623/1‑94 (MQ=60)
                                                GCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCC                                             >  SRR4302533.5519603/1‑94 (MQ=60)
                                                  TGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCG                                           <  SRR4302533.3187508/94‑1 (MQ=60)
                                                  TGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCG                                           >  SRR4302533.4322992/1‑94 (MQ=60)
                                                  TGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGCTCGCCCG                                           >  SRR4302533.2736549/1‑94 (MQ=60)
                                                   GCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGT                                          >  SRR4302533.1591911/1‑94 (MQ=60)
                                                    CATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTG                                         <  SRR4302533.4035468/94‑1 (MQ=60)
                                                    CATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTG                                         <  SRR4302533.605354/94‑1 (MQ=60)
                                                    CATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTG                                         >  SRR4302533.3181333/1‑94 (MQ=60)
                                                     ATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGA                                        >  SRR4302533.5469617/1‑94 (MQ=60)
                                                      TCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAG                                       <  SRR4302533.746477/94‑1 (MQ=60)
                                                      TCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAG                                       >  SRR4302533.2426399/1‑94 (MQ=60)
                                                          TCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGA                                   <  SRR4302533.2220775/94‑1 (MQ=60)
                                                           CCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAG                                  >  SRR4302533.5128820/1‑94 (MQ=60)
                                                           CCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAG                                  >  SRR4302533.725319/1‑94 (MQ=60)
                                                           CCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAG                                  <  SRR4302533.1719408/94‑1 (MQ=60)
                                                            CGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGC                                 >  SRR4302533.4479706/1‑94 (MQ=60)
                                                            CGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGC                                 >  SRR4302533.2221263/1‑94 (MQ=60)
                                                             GTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCA                                <  SRR4302533.1672650/94‑1 (MQ=60)
                                                             GTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCA                                >  SRR4302533.3538694/1‑94 (MQ=60)
                                                               CTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACG                              <  SRR4302533.5238863/94‑1 (MQ=60)
                                                               CTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACG                              <  SRR4302533.4596420/94‑1 (MQ=60)
                                                               CTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACG                              >  SRR4302533.6020080/1‑94 (MQ=60)
                                                                TTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGC                             >  SRR4302533.6077921/1‑94 (MQ=60)
                                                                 TCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCT                            <  SRR4302533.2184343/94‑1 (MQ=60)
                                                                   CGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTT                          <  SRR4302533.5002697/94‑1 (MQ=60)
                                                                   CGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTT                          >  SRR4302533.4714389/1‑94 (MQ=60)
                                                                   CGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTT                          >  SRR4302533.2057338/1‑94 (MQ=60)
                                                                    GCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTC                         <  SRR4302533.723090/94‑1 (MQ=60)
                                                                    GCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTC                         <  SRR4302533.3384106/94‑1 (MQ=60)
                                                                     CGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCG                        <  SRR4302533.741035/94‑1 (MQ=60)
                                                                     CGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCG                        <  SRR4302533.984689/94‑1 (MQ=60)
                                                                     CGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCG                        >  SRR4302533.3843218/1‑94 (MQ=60)
                                                                      GCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGG                       <  SRR4302533.5841951/94‑1 (MQ=60)
                                                                       CAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGC                      >  SRR4302533.4204897/1‑94 (MQ=60)
                                                                       CAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGC                      >  SRR4302533.5962845/1‑94 (MQ=60)
                                                                        AGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCA                     >  SRR4302533.4046510/1‑94 (MQ=60)
                                                                         GAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCAC                    >  SRR4302533.5043873/1‑94 (MQ=60)
                                                                         GAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCAC                    <  SRR4302533.1681430/94‑1 (MQ=60)
                                                                         GAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCAC                    <  SRR4302533.1708830/94‑1 (MQ=60)
                                                                          AAAACCGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACG                   <  SRR4302533.799379/94‑1 (MQ=60)
                                                                          AAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACG                   >  SRR4302533.5345173/1‑94 (MQ=60)
                                                                          AAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACG                   >  SRR4302533.1107500/1‑94 (MQ=60)
                                                                          AAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACG                   >  SRR4302533.2226707/1‑94 (MQ=60)
                                                                           AAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGT                  >  SRR4302533.5241866/1‑94 (MQ=60)
                                                                             AGCGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTAC                <  SRR4302533.3349661/94‑1 (MQ=60)
                                                                             AACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTAC                >  SRR4302533.1045975/1‑94 (MQ=60)
                                                                             AACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTAC                <  SRR4302533.651336/94‑1 (MQ=60)
                                                                              ACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACC               <  SRR4302533.4674726/94‑1 (MQ=60)
                                                                               CGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCG              <  SRR4302533.2962245/94‑1 (MQ=60)
                                                                                 CATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCTGCACGTACCGGC            <  SRR4302533.3184261/94‑1 (MQ=60)
                                                                                 CATCGGCCTGGCATTGTGCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGC            <  SRR4302533.1337345/94‑1 (MQ=60)
                                                                                   TCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTT          >  SRR4302533.4403102/1‑94 (MQ=60)
                                                                                    CGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTCCCGGCTTT         >  SRR4302533.2139743/1‑94 (MQ=60)
                                                                                    CGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTT         <  SRR4302533.1546424/94‑1 (MQ=60)
                                                                                     GGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTG        <  SRR4302533.2287282/94‑1 (MQ=60)
                                                                                      GCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGA       <  SRR4302533.243159/94‑1 (MQ=60)
                                                                                       CCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAG      >  SRR4302533.1549132/1‑94 (MQ=60)
                                                                                        CTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGT     >  SRR4302533.2048129/1‑94 (MQ=60)
                                                                                        CTGGCATTGTTCGGTAAGGGCAATATCGGGTCCCGTTGGCTGGAACTGTTCGCCAGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGT     >  SRR4302533.5677872/1‑94 (MQ=60)
                                                                                         TGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTT    <  SRR4302533.2612134/94‑1 (MQ=60)
                                                                                         TGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTT    >  SRR4302533.1324393/1‑94 (MQ=60)
                                                                                          GGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTT   >  SRR4302533.2061230/1‑94 (MQ=60)
                                                                                           GCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTG  >  SRR4302533.5552816/1‑94 (MQ=60)
                                                                                            |                                                                                            
CCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTG  >  NC_000913/4128707‑4128891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑