Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,140,312 G→A M68I (ATG→ATA)  frwC → PTS system fructose‑like enzyme IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,140,3120GA99.5% 1578.0 / ‑4.7 429M68I (ATG→ATA) frwCPTS system fructose‑like enzyme IIC component
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (220/207);  total (221/208)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

Only 100 of 443 total aligned reads displayed.

GTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAG  >  NC_000913/4140222‑4140401
                                                                                          |                                                                                         
gttgtATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGt                                                                                         >  2:8258157/1‑93 (MQ=255)
gttgtATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGt                                                                                         <  1:3032948/93‑1 (MQ=255)
gttgtATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGt                                                                                         <  1:8396109/93‑1 (MQ=255)
 ttgtATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTg                                                                                        <  1:455581/93‑1 (MQ=255)
 ttgtATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTg                                                                                        >  1:7394934/1‑93 (MQ=255)
      tGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCttt                                                                                   >  2:3559830/1‑93 (MQ=255)
          aaaGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTc                                                                               <  2:7277761/93‑1 (MQ=255)
          aaaGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTc                                                                               <  2:6567236/93‑1 (MQ=255)
           aaGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCg                                                                              >  2:8859669/1‑93 (MQ=255)
           aaGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCg                                                                              >  2:6764633/1‑93 (MQ=255)
            aGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGc                                                                             <  1:9387512/93‑1 (MQ=255)
             ggCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCa                                                                            >  1:7077778/1‑93 (MQ=255)
             ggCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCa                                                                            >  1:4991809/1‑93 (MQ=255)
                gcAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCg                                                                         >  2:5306292/1‑93 (MQ=255)
                 cAGGGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGt                                                                        <  2:2988749/93‑1 (MQ=255)
                  aGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTa                                                                       <  1:6683311/93‑1 (MQ=255)
                  aGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTa                                                                       >  2:9474194/1‑93 (MQ=255)
                    tGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACa                                                                     >  2:1573506/1‑93 (MQ=255)
                    tGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACa                                                                     >  2:3198412/1‑93 (MQ=255)
                     gCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACAt                                                                    >  2:1726600/1‑93 (MQ=255)
                      ccGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATc                                                                   >  1:4230843/1‑93 (MQ=255)
                       cGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCg                                                                  <  2:1781094/93‑1 (MQ=255)
                       cGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCg                                                                  <  1:1186491/93‑1 (MQ=255)
                        ggATGCCTTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCgg                                                                 <  2:4643330/93‑1 (MQ=255)
                        ggATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCgg                                                                 >  2:1014876/1‑93 (MQ=255)
                         gATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGt                                                                >  1:4087368/1‑93 (MQ=255)
                         gATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGt                                                                >  1:5877021/1‑93 (MQ=255)
                           tGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTa                                                              <  2:9714734/93‑1 (MQ=255)
                            gCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTAt                                                             <  1:7665984/93‑1 (MQ=255)
                              cGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTc                                                           <  1:7763129/93‑1 (MQ=255)
                               gTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTcc                                                          >  1:359736/1‑93 (MQ=255)
                                tAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCa                                                         <  1:1957018/93‑1 (MQ=255)
                                tAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCa                                                         <  1:8635761/93‑1 (MQ=255)
                                 aGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAt                                                        <  1:8503317/93‑1 (MQ=255)
                                  gCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAtt                                                       <  2:3946576/93‑1 (MQ=255)
                                    cGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGc                                                     <  2:4508863/93‑1 (MQ=255)
                                    cGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGc                                                     <  2:8412103/93‑1 (MQ=255)
                                     gATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACCTCGGTTATTCCATTGCa                                                    >  1:2068235/1‑93 (MQ=255)
                                      aTCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCag                                                   >  2:549674/1‑93 (MQ=255)
                                      aTCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCag                                                   >  2:2463057/1‑93 (MQ=255)
                                       tCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCaga                                                  >  2:513236/1‑93 (MQ=255)
                                       tCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCaga                                                  >  2:6178411/1‑93 (MQ=255)
                                       tCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCaga                                                  >  2:640795/1‑93 (MQ=255)
                                       tCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCaga                                                  >  1:3682982/1‑93 (MQ=255)
                                        ccAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCagag                                                 >  1:2717103/1‑93 (MQ=255)
                                         cAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGc                                                >  2:3295908/1‑93 (MQ=255)
                                          aaaTCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCg                                               >  1:2896341/1‑93 (MQ=255)
                                          aaaTCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCg                                               >  2:2104506/1‑93 (MQ=255)
                                           aaTCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGt                                              >  1:2824543/1‑93 (MQ=255)
                                            aTCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGtt                                             <  2:7664830/93‑1 (MQ=255)
                                             tCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTc                                            >  1:9433606/1‑93 (MQ=255)
                                              cTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCt                                           >  2:3453922/1‑93 (MQ=255)
                                               tGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTg                                          >  2:9611019/1‑93 (MQ=255)
                                                  aaaaaCTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTgcgc                                       <  1:8878092/93‑1 (MQ=255)
                                                   aaaaCTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCt                                      <  2:7384789/93‑1 (MQ=255)
                                                   aaaaCTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCt                                      <  1:7986274/93‑1 (MQ=255)
                                                    aaaCTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTg                                     <  2:4197571/93‑1 (MQ=255)
                                                    aaaCTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTg                                     <  1:9050231/93‑1 (MQ=255)
                                                     aaCTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTgg                                    >  1:4806998/1‑93 (MQ=255)
                                                      aCTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGc                                   >  2:119603/1‑93 (MQ=255)
                                                      aCTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGc                                   <  2:8584718/93‑1 (MQ=255)
                                                        tGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTc                                 >  2:9196332/1‑93 (MQ=255)
                                                        tGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTc                                 >  1:8775489/1‑93 (MQ=255)
                                                        tGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTc                                 >  1:5256971/1‑93 (MQ=255)
                                                         gTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTcc                                >  1:623587/1‑93 (MQ=255)
                                                         gTTTGATATCGGCGTTGCAGGCTTGACGGTGATAGTGCCTTTCCTCGAAGCGTACATCGGTTACTCCATTGCAGAGCGTTCTGCGCTGGCTcc                                <  1:2285805/93‑1 (MQ=255)
                                                             gATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTgc                            <  1:5541787/93‑1 (MQ=255)
                                                             gATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTgc                            >  1:1367031/1‑93 (MQ=255)
                                                              atatCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTgcg                           >  2:3615268/1‑93 (MQ=255)
                                                                 tCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCtt                        >  1:6396358/1‑92 (MQ=255)
                                                                 tCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTa                        <  2:2598020/93‑1 (MQ=255)
                                                                 tCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTa                        <  2:5909517/93‑1 (MQ=255)
                                                                 tCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTa                        >  1:83004/1‑93 (MQ=255)
                                                                 tCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTa                        <  2:7923041/93‑1 (MQ=255)
                                                                  cGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTAt                       <  1:256979/93‑1 (MQ=255)
                                                                  cGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGAAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGTGCTGGCTGCGTGCGCTAt                       >  1:2180978/1‑93 (MQ=255)
                                                                   ggCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATc                      <  1:9726326/93‑1 (MQ=255)
                                                                   ggCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATc                      >  1:9901447/1‑93 (MQ=255)
                                                                   ggCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATc                      >  2:9052092/1‑93 (MQ=255)
                                                                    gCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCg                     >  1:6060191/1‑93 (MQ=255)
                                                                     cGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCgg                    <  2:1348327/93‑1 (MQ=255)
                                                                      gTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGt                   <  2:7136355/93‑1 (MQ=255)
                                                                       ttGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTAt                       >  1:1024166/1‑88 (MQ=255)
                                                                       ttGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTAt                       <  2:5720931/88‑1 (MQ=255)
                                                                        tGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGc                 >  1:694793/1‑93 (MQ=255)
                                                                         gCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATGCCATTGCAGAGCGTTCTGCGCTGGTTCCGTGCTCTATCGGTGcc                <  2:228960/93‑1 (MQ=255)
                                                                          cGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCt               <  1:3956053/93‑1 (MQ=255)
                                                                           gggCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTg              <  2:5390550/93‑1 (MQ=255)
                                                                            ggCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTgg             <  1:1752167/93‑1 (MQ=255)
                                                                            ggCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTgg             >  2:188912/1‑93 (MQ=255)
                                                                                tGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTg         <  2:287520/93‑1 (MQ=255)
                                                                                tGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTg         <  2:8204215/93‑1 (MQ=255)
                                                                                  aCGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGt       >  1:6491266/1‑93 (MQ=255)
                                                                                  aCGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGt       >  1:3351849/1‑93 (MQ=255)
                                                                                   cGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTa      <  2:2759494/93‑1 (MQ=255)
                                                                                    gCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTaa     >  2:7586004/1‑93 (MQ=255)
                                                                                     cTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAAc    <  1:3157600/93‑1 (MQ=255)
                                                                                     cTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAAc    <  2:4829054/93‑1 (MQ=255)
                                                                                     cTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAAc    >  2:5846642/1‑93 (MQ=255)
                                                                                      tGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACa   >  1:7709762/1‑93 (MQ=255)
                                                                                          |                                                                                         
GTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAG  >  NC_000913/4140222‑4140401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 235 total aligned reads displayed.
ATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAGCTTT  >  NC_000913/4140220‑4140405
                                                                                            |                                                                                             
ATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAG                                                                                              >  SRR4302540.4298420/1‑94 (MQ=60)
ATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAG                                                                                              >  SRR4302540.4496248/1‑94 (MQ=60)
ATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAG                                                                                              <  SRR4302540.9643707/94‑1 (MQ=60)
 TGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGT                                                                                             <  SRR4302540.2345146/94‑1 (MQ=60)
 TGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGT                                                                                             <  SRR4302540.4712838/94‑1 (MQ=60)
 TGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGT                                                                                             <  SRR4302540.2391766/94‑1 (MQ=60)
 TGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGT                                                                                             <  SRR4302540.6016373/94‑1 (MQ=60)
 TGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGT                                                                                             <  SRR4302540.6016374/94‑1 (MQ=60)
  GTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTG                                                                                            <  SRR4302540.3066241/94‑1 (MQ=60)
  GTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTG                                                                                            <  SRR4302540.8493539/94‑1 (MQ=60)
  GTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTG                                                                                            >  SRR4302540.7477330/1‑94 (MQ=60)
   TTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGC                                                                                           >  SRR4302540.5633526/1‑94 (MQ=60)
   TTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGC                                                                                           <  SRR4302540.460443/94‑1 (MQ=60)
    TGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCC                                                                                          <  SRR4302540.1163827/94‑1 (MQ=60)
       ATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTT                                                                                       >  SRR4302540.2710662/1‑94 (MQ=60)
        TGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTC                                                                                      >  SRR4302540.8494567/1‑94 (MQ=60)
         GGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCC                                                                                     >  SRR4302540.8668323/1‑94 (MQ=60)
           GAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTC                                                                                   <  SRR4302540.3415022/94‑1 (MQ=60)
           CAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTC                                                                                   >  SRR4302540.7765442/1‑94 (MQ=60)
           CAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGGGGCGGGCCTGACGCTGATAGTGCCTTTCCCC                                                                                   >  SRR4302540.7765443/1‑94 (MQ=60)
             AAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGC                                                                                 >  SRR4302540.6778039/1‑94 (MQ=60)
              AGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCA                                                                                <  SRR4302540.9499241/94‑1 (MQ=60)
              AGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCA                                                                                >  SRR4302540.7156541/1‑94 (MQ=60)
              AGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCA                                                                                >  SRR4302540.5045856/1‑94 (MQ=60)
               GGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAG                                                                               <  SRR4302540.6108038/94‑1 (MQ=60)
                 CGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGCG                                                                             >  SRR4302540.9376721/1‑94 (MQ=60)
                 CGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCG                                                                             <  SRR4302540.4108012/94‑1 (MQ=60)
                 CGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCG                                                                             >  SRR4302540.7098934/1‑94 (MQ=60)
                  GCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGT                                                                            <  SRR4302540.3379569/94‑1 (MQ=60)
                   CAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTA                                                                           >  SRR4302540.10033396/1‑94 (MQ=60)
                    AGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTAC                                                                          <  SRR4302540.2506244/94‑1 (MQ=60)
                    AGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTAC                                                                          <  SRR4302540.6757125/94‑1 (MQ=60)
                    AGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTAC                                                                          >  SRR4302540.10062603/1‑94 (MQ=60)
                     GTGCCGGATGCCGTAGACGATCCGAATCTGAAAAAACTCTTTGATATCGGCGTTGCGGGCTTGACGCTGATACTGCCTTTCCTCGCAGCGCACA                                                                         >  SRR4302540.3663220/1‑94 (MQ=60)
                      TGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACAT                                                                        >  SRR4302540.8475210/1‑94 (MQ=60)
                       GCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATC                                                                       >  SRR4302540.4276900/1‑94 (MQ=60)
                       GCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATC                                                                       <  SRR4302540.9523855/94‑1 (MQ=60)
                       GCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATC                                                                       >  SRR4302540.9001413/1‑94 (MQ=60)
                         CGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGG                                                                     >  SRR4302540.1751238/1‑94 (MQ=60)
                         CGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGG                                                                     <  SRR4302540.1198731/94‑1 (MQ=60)
                         CGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGG                                                                     <  SRR4302540.5459680/94‑1 (MQ=60)
                          GGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGGGGGCTTGACGCTGATAGGGCCTTTCCTCGCAGCGGACATCGGG                                                                    >  SRR4302540.4131451/1‑94 (MQ=60)
                          GGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGT                                                                    >  SRR4302540.4472359/1‑94 (MQ=60)
                          GGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGT                                                                    >  SRR4302540.4131449/1‑94 (MQ=60)
                          GGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGT                                                                    >  SRR4302540.5941983/1‑94 (MQ=60)
                            ATGCCGTAGCCGATCCAAATCTGAAAACTCTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTA                                                                  <  SRR4302540.1382199/94‑1 (MQ=60)
                            ATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATTGGTTA                                                                  >  SRR4302540.288923/1‑94 (MQ=60)
                            ATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTA                                                                  >  SRR4302540.6736941/1‑94 (MQ=60)
                            ATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTA                                                                  <  SRR4302540.8788753/94‑1 (MQ=60)
                            ATGCCGTAGCCGATCCAAATCTAAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGCGCCTTTCCTCGCAGCGTACCTTGGTTA                                                                  >  SRR4302540.288922/1‑94 (MQ=60)
                             TGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTAT                                                                 >  SRR4302540.2835882/1‑94 (MQ=60)
                             TGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTAT                                                                 >  SRR4302540.4743253/1‑94 (MQ=60)
                             TGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTAT                                                                 >  SRR4302540.3508638/1‑94 (MQ=60)
                              GCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATT                                                                <  SRR4302540.7752354/94‑1 (MQ=60)
                               CCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTC                                                               <  SRR4302540.3100180/94‑1 (MQ=60)
                               CCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTC                                                               >  SRR4302540.8792791/1‑94 (MQ=60)
                                CGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCC                                                              <  SRR4302540.7853862/94‑1 (MQ=60)
                                CGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCC                                                              >  SRR4302540.363433/1‑94 (MQ=60)
                                 GTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGGTAGTGCATTTCATCGCAGCGTACAACAGTTATTTCA                                                             >  SRR4302540.9577780/1‑94 (MQ=60)
                                 GTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCA                                                             >  SRR4302540.8920571/1‑94 (MQ=60)
                                 GTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCA                                                             >  SRR4302540.8920572/1‑94 (MQ=60)
                                  TAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAT                                                            <  SRR4302540.8736069/94‑1 (MQ=60)
                                  TAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAT                                                            <  SRR4302540.6159316/94‑1 (MQ=60)
                                  TAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAT                                                            <  SRR4302540.1978295/94‑1 (MQ=60)
                                  TAGCCGATCCAAATATGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAT                                                            <  SRR4302540.6158996/94‑1 (MQ=60)
                                   AGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACCTCGGCTATTCCCTT                                                           >  SRR4302540.2828068/1‑94 (MQ=60)
                                   AGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT                                                           <  SRR4302540.7908466/94‑1 (MQ=60)
                                   AGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT                                                           >  SRR4302540.285895/1‑94 (MQ=60)
                                   AGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT                                                           <  SRR4302540.2966450/94‑1 (MQ=60)
                                   AGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT                                                           <  SRR4302540.8602134/94‑1 (MQ=60)
                                   AGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT                                                           <  SRR4302540.7696198/94‑1 (MQ=60)
                                    GCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTG                                                          <  SRR4302540.808698/94‑1 (MQ=60)
                                    GCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTG                                                          <  SRR4302540.8659473/94‑1 (MQ=60)
                                    GCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTG                                                          <  SRR4302540.2004668/94‑1 (MQ=60)
                                    GCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTG                                                          <  SRR4302540.1956275/94‑1 (MQ=60)
                                    GCAGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTG                                                          <  SRR4302540.7409580/94‑1 (MQ=60)
                                    ACCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTG                                                          <  SRR4302540.310752/94‑1 (MQ=60)
                                      CGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACCTCGGTTATTCCATTGCA                                                        >  SRR4302540.2090480/1‑94 (MQ=60)
                                      CGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCA                                                        >  SRR4302540.3001099/1‑94 (MQ=60)
                                      CGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCA                                                        >  SRR4302540.3184087/1‑94 (MQ=60)
                                      CGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCA                                                        <  SRR4302540.9212414/94‑1 (MQ=60)
                                      CGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTGTCCTCGCACCGAACATCGGTTATTCCATTGCA                                                        >  SRR4302540.3001100/1‑94 (MQ=60)
                                       GATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAG                                                       <  SRR4302540.1238382/94‑1 (MQ=60)
                                       GATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAG                                                       >  SRR4302540.6360853/1‑94 (MQ=60)
                                       GATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAG                                                       >  SRR4302540.49909/1‑94 (MQ=60)
                                       GATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAG                                                       <  SRR4302540.2297779/94‑1 (MQ=60)
                                       GATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAG                                                       >  SRR4302540.9053094/1‑94 (MQ=60)
                                       GATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAG                                                       >  SRR4302540.3715659/1‑94 (MQ=60)
                                        ATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGA                                                      <  SRR4302540.10040347/94‑1 (MQ=60)
                                        ATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGA                                                      >  SRR4302540.2169948/1‑94 (MQ=60)
                                         TCCACATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGAAAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCCGAG                                                     >  SRR4302540.4115479/1‑94 (MQ=60)
                                         TCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAG                                                     <  SRR4302540.7648012/94‑1 (MQ=60)
                                         TCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAG                                                     <  SRR4302540.5361069/94‑1 (MQ=60)
                                          TCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGC                                                    <  SRR4302540.3664657/94‑1 (MQ=60)
                                          CCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGC                                                    >  SRR4302540.9711354/1‑94 (MQ=60)
                                           CAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCG                                                   >  SRR4302540.2927924/1‑94 (MQ=60)
                                           CAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCG                                                   >  SRR4302540.3932248/1‑94 (MQ=60)
                                            AAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGT                                                  >  SRR4302540.5086746/1‑94 (MQ=60)
                                            AAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGT                                                  >  SRR4302540.4787836/1‑94 (MQ=60)
                                            AAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGT                                                  >  SRR4302540.7073497/1‑94 (MQ=60)
                                            AAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGT                                                  >  SRR4302540.2855063/1‑94 (MQ=60)
                                            AAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGATGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGT                                                  >  SRR4302540.8563357/1‑94 (MQ=60)
                                             AATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTT                                                 <  SRR4302540.819291/94‑1 (MQ=60)
                                              ATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTC                                                <  SRR4302540.212026/94‑1 (MQ=60)
                                              ATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTC                                                >  SRR4302540.9545652/1‑94 (MQ=60)
                                               TCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCT                                               <  SRR4302540.652324/94‑1 (MQ=60)
                                               TCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCT                                               <  SRR4302540.2834832/94‑1 (MQ=60)
                                               TCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCT                                               >  SRR4302540.8517886/1‑94 (MQ=60)
                                               TCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCT                                               <  SRR4302540.2704972/94‑1 (MQ=60)
                                                TTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTG                                              <  SRR4302540.5439665/94‑1 (MQ=60)
                                                CTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTG                                              <  SRR4302540.9951098/94‑1 (MQ=60)
                                                 TGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGC                                             <  SRR4302540.2808685/94‑1 (MQ=60)
                                                  GAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCG                                            >  SRR4302540.8183218/1‑94 (MQ=60)
                                                   AAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGC                                           <  SRR4302540.6521615/94‑1 (MQ=60)
                                                   AAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGATTATTCCATTGCAGAGCGTTCTGCGC                                           >  SRR4302540.7814587/1‑94 (MQ=60)
                                                    AAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCT                                          <  SRR4302540.8980832/94‑1 (MQ=60)
                                                     AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTG                                         <  SRR4302540.8079271/94‑1 (MQ=60)
                                                     AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTG                                         <  SRR4302540.3206270/94‑1 (MQ=60)
                                                     AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTG                                         <  SRR4302540.4912198/94‑1 (MQ=60)
                                                      AAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGG                                        >  SRR4302540.7008072/1‑94 (MQ=60)
                                                      AAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGG                                        <  SRR4302540.9158873/94‑1 (MQ=60)
                                                       AACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGC                                       <  SRR4302540.7846517/94‑1 (MQ=60)
                                                       AACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGC                                       >  SRR4302540.8118484/1‑94 (MQ=60)
                                                       AACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGC                                       >  SRR4302540.2257971/1‑94 (MQ=60)
                                                        ACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCT                                      >  SRR4302540.387651/1‑94 (MQ=60)
                                                        ACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCT                                      >  SRR4302540.9771671/1‑94 (MQ=60)
                                                         CTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTC                                     <  SRR4302540.1323378/94‑1 (MQ=60)
                                                         CTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTC                                     >  SRR4302540.5314328/1‑94 (MQ=60)
                                                         CTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTC                                     >  SRR4302540.8877139/1‑94 (MQ=60)
                                                         CTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTC                                     >  SRR4302540.7785238/1‑94 (MQ=60)
                                                         CTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTC                                     >  SRR4302540.6399045/1‑94 (MQ=60)
                                                         CTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGAGTTCTGGGCTGGCTC                                     >  SRR4302540.6399046/1‑94 (MQ=60)
                                                          TGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCC                                    >  SRR4302540.630225/1‑94 (MQ=60)
                                                          TGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCC                                    >  SRR4302540.630226/1‑94 (MQ=60)
                                                           GTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCG                                   >  SRR4302540.1330126/1‑94 (MQ=60)
                                                           GTTTGATATCGGCGTTGCAGGCTTGACGGTGATAGTGCCTTTCCTCGAAGCGTACATCGGTTACTCCATTGCAGAGCGTTCTGCGCTGGCTCCG                                   <  SRR4302540.2310380/94‑1 (MQ=60)
                                                            TTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGT                                  <  SRR4302540.803303/94‑1 (MQ=60)
                                                             TTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTG                                 <  SRR4302540.8843947/94‑1 (MQ=60)
                                                              TGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGC                                >  SRR4302540.9354127/1‑94 (MQ=60)
                                                              TGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGC                                >  SRR4302540.7834597/1‑94 (MQ=60)
                                                                  ATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTT                            >  SRR4302540.6467568/1‑94 (MQ=60)
                                                                  ATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTA                            >  SRR4302540.3057925/1‑94 (MQ=60)
                                                                  ATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTA                            >  SRR4302540.83879/1‑94 (MQ=60)
                                                                  ATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTA                            >  SRR4302540.4645930/1‑94 (MQ=60)
                                                                  ATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTA                            >  SRR4302540.1838545/1‑94 (MQ=60)
                                                                   TCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTAT                           >  SRR4302540.2713904/1‑94 (MQ=60)
                                                                   TCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTAT                           >  SRR4302540.2204502/1‑94 (MQ=60)
                                                                   TCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGAAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGTGCTGGCTGCGTGCGCTAT                           >  SRR4302540.2204503/1‑94 (MQ=60)
                                                                    tcGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATC                          <  SRR4302540.3647530/92‑1 (MQ=60)
                                                                    CGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATC                          >  SRR4302540.9543588/1‑94 (MQ=60)
                                                                    CGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATC                          <  SRR4302540.259471/94‑1 (MQ=60)
                                                                    CGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATC                          <  SRR4302540.6100219/94‑1 (MQ=60)
                                                                    CGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATC                          >  SRR4302540.10017124/1‑94 (MQ=60)
                                                                    CGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATC                          >  SRR4302540.7118700/1‑94 (MQ=60)
                                                                     GGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCG                         <  SRR4302540.3918250/94‑1 (MQ=60)
                                                                     GGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCG                         >  SRR4302540.6127544/1‑94 (MQ=60)
                                                                     GGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCG                         <  SRR4302540.9840563/94‑1 (MQ=60)
                                                                      GCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGG                        <  SRR4302540.9658957/94‑1 (MQ=60)
                                                                      GCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGG                        <  SRR4302540.5765600/94‑1 (MQ=60)
                                                                      GCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGG                        <  SRR4302540.2062277/94‑1 (MQ=60)
                                                                      GCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGG                        <  SRR4302540.4840061/94‑1 (MQ=60)
                                                                      GCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGG                        >  SRR4302540.7414523/1‑94 (MQ=60)
                                                                       CGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGT                       >  SRR4302540.3246053/1‑94 (MQ=60)
                                                                       CGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGT                       <  SRR4302540.145152/94‑1 (MQ=60)
                                                                        GTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCaagc                      >  SRR4302540.1034555/1‑90 (MQ=60)
                                                                        GTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCaagc                      >  SRR4302540.5783903/1‑90 (MQ=60)
                                                                        GTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTG                      <  SRR4302540.3929890/94‑1 (MQ=60)
                                                                        GTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTG                      >  SRR4302540.2303287/1‑94 (MQ=60)
                                                                        GTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTG                      <  SRR4302540.4169803/94‑1 (MQ=60)
                                                                        GTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTG                      <  SRR4302540.8424516/94‑1 (MQ=60)
                                                                        GTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTC                      >  SRR4302540.7531882/1‑94 (MQ=60)
                                                                         TTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGC                     >  SRR4302540.702245/1‑94 (MQ=60)
                                                                          TGCGGGCTTCACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCC                    <  SRR4302540.4873482/94‑1 (MQ=60)
                                                                           GCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCT                   <  SRR4302540.6806258/94‑1 (MQ=60)
                                                                            CGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTG                  <  SRR4302540.3998741/94‑1 (MQ=60)
                                                                            CGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTG                  >  SRR4302540.9496463/1‑94 (MQ=60)
                                                                            CGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTG                  >  SRR4302540.5399757/1‑94 (MQ=60)
                                                                             GGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGG                 >  SRR4302540.10080856/1‑94 (MQ=60)
                                                                             GGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGG                 >  SRR4302540.8418475/1‑94 (MQ=60)
                                                                             GGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGG                 >  SRR4302540.1592229/1‑94 (MQ=60)
                                                                              GGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGG                <  SRR4302540.1771148/94‑1 (MQ=60)
                                                                              GGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGG                >  SRR4302540.7426808/1‑94 (MQ=60)
                                                                               GCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGT               >  SRR4302540.5268778/1‑94 (MQ=60)
                                                                                 TTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTG             <  SRR4302540.4284775/94‑1 (MQ=60)
                                                                                  TGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGG            <  SRR4302540.4154607/94‑1 (MQ=60)
                                                                                   GACGCTGATCGTGCCTTTCCTCGCCGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGTCTCCGTGCGCTATCGcggcctgcgttgct           >  SRR4302540.6563455/1‑80 (MQ=60)
                                                                                   GACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGT           >  SRR4302540.6563457/1‑94 (MQ=60)
                                                                                   GACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGT           >  SRR4302540.3388589/1‑94 (MQ=60)
                                                                                   GACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGT           >  SRR4302540.1124231/1‑94 (MQ=60)
                                                                                      GCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAAC        >  SRR4302540.8820269/1‑94 (MQ=60)
                                                                                      GCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAAC        >  SRR4302540.9879568/1‑94 (MQ=60)
                                                                                      GCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAAC        <  SRR4302540.6038134/94‑1 (MQ=60)
                                                                                       CTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACA       <  SRR4302540.3191964/94‑1 (MQ=60)
                                                                                       CTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACA       <  SRR4302540.652090/94‑1 (MQ=60)
                                                                                        TGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAG      >  SRR4302540.2030702/1‑94 (MQ=60)
                                                                                        TGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAG      >  SRR4302540.2385982/1‑94 (MQ=60)
                                                                                        TGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAG      >  SRR4302540.6734631/1‑94 (MQ=60)
                                                                                          ATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAGCT    <  SRR4302540.688542/94‑1 (MQ=60)
                                                                                           TAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGCCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAGCTT   <  SRR4302540.4405501/94‑1 (MQ=60)
                                                                                            AGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAGCTTT  <  SRR4302540.8608255/94‑1 (MQ=60)
                                                                                            |                                                                                             
ATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAGCTTT  >  NC_000913/4140220‑4140405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑