Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,140,312 G→A M68I (ATG→ATA)  frwC → PTS system fructose‑like enzyme IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,140,3120GA99.6% 880.5 / NA 235M68I (ATG→ATA) frwCPTS system fructose‑like enzyme IIC component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (135/99);  minor base T (1/0);  total (136/99)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 240 total aligned reads displayed.

GTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAG  >  NC_000913/4140222‑4140401
                                                                                           |                                                                                          
gttgtATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGt                                                                                          <  2:2975571/93‑1 (MQ=255)
 ttgtATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTg                                                                                         >  1:5092788/1‑93 (MQ=255)
   gtATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAGATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGcc                                                                                       <  1:2161555/93‑1 (MQ=255)
   gtATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGcc                                                                                       <  2:5046796/93‑1 (MQ=255)
    tATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCt                                                                                      >  1:2594809/1‑93 (MQ=255)
     aTGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCtt                                                                                     <  1:2489904/93‑1 (MQ=255)
      tGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCttt                                                                                    >  1:5153614/1‑93 (MQ=255)
      tGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCttt                                                                                    >  1:2249144/1‑93 (MQ=255)
       ggCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTc                                                                                   <  2:1580066/93‑1 (MQ=255)
         cAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCt                                                                                 <  1:3949851/93‑1 (MQ=255)
         cAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCt                                                                                 <  2:3798915/93‑1 (MQ=255)
          aaaGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTc                                                                                <  2:800863/93‑1 (MQ=255)
          aaaGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTc                                                                                <  1:577462/93‑1 (MQ=255)
          aaaGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTc                                                                                >  1:173353/1‑93 (MQ=255)
           aaGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCg                                                                               >  1:6197245/1‑93 (MQ=255)
           aaGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCg                                                                               <  2:4543029/93‑1 (MQ=255)
           aaGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCg                                                                               >  1:1681855/1‑93 (MQ=255)
             ggCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCa                                                                             <  1:3019938/93‑1 (MQ=255)
                gcAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGTCTTTCCTCGCAGCg                                                                          >  2:5483897/1‑93 (MQ=255)
                 cAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGt                                                                         <  1:1971180/93‑1 (MQ=255)
                  aGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTa                                                                        >  2:5988439/1‑93 (MQ=255)
                   gTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTAc                                                                       <  2:3099431/93‑1 (MQ=255)
                   gTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTAc                                                                       >  2:6170471/1‑93 (MQ=255)
                   gTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTAc                                                                       <  2:5261525/93‑1 (MQ=255)
                   gTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTAc                                                                       <  1:2759709/93‑1 (MQ=255)
                    tGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACa                                                                      >  2:5321858/1‑93 (MQ=255)
                    tGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACa                                                                      >  1:1665591/1‑93 (MQ=255)
                    tGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACa                                                                      <  2:4412362/93‑1 (MQ=255)
                    tGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACa                                                                      >  1:524176/1‑93 (MQ=255)
                     gCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACAt                                                                     <  1:826020/93‑1 (MQ=255)
                     gCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACAt                                                                     <  1:5894317/93‑1 (MQ=255)
                      ccGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATc                                                                    <  2:4193237/93‑1 (MQ=255)
                         gATGCCTTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGt                                                                 <  1:2301924/93‑1 (MQ=255)
                         gATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGt                                                                 >  1:2657503/1‑93 (MQ=255)
                         gATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGt                                                                 >  1:4518011/1‑93 (MQ=255)
                           ttccGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTa                                                               <  1:3161877/91‑1 (MQ=255)
                           tGCCGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTa                                                               <  1:1966709/93‑1 (MQ=255)
                             ccGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTAtt                                                             <  2:2537743/93‑1 (MQ=255)
                             ccGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACAGCGGTTAtt                                                             <  2:6138822/93‑1 (MQ=255)
                              cGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTc                                                            <  2:1754081/93‑1 (MQ=255)
                                tAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCa                                                          >  1:4624002/1‑93 (MQ=255)
                                tAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCa                                                          <  1:3096006/93‑1 (MQ=255)
                                 aGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAt                                                         >  2:2379187/1‑93 (MQ=255)
                                 aGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAt                                                         >  2:1385064/1‑93 (MQ=255)
                                  gCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAtt                                                        >  2:3646140/1‑93 (MQ=255)
                                  gCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAtt                                                        >  2:524341/1‑93 (MQ=255)
                                   ccGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑g                                                      >  1:2999271/1‑93 (MQ=255)
                                    cGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑Gc                                                     >  1:937265/1‑93 (MQ=255)
                                    cGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑Gc                                                     <  1:1002830/93‑1 (MQ=255)
                                     gATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCa                                                    >  2:3234787/1‑93 (MQ=255)
                                     gATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCa                                                    >  2:4349816/1‑93 (MQ=255)
                                     gATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCa                                                    <  1:5393371/93‑1 (MQ=255)
                                       tCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCaga                                                  <  2:711182/93‑1 (MQ=255)
                                       tCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCaga                                                  >  2:5900635/1‑93 (MQ=255)
                                       tCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCaga                                                  <  2:2579694/93‑1 (MQ=255)
                                        ccAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCagag                                                 >  1:4137130/1‑93 (MQ=255)
                                         cAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGc                                                >  1:3335106/1‑93 (MQ=255)
                                         cAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGc                                                >  2:1944936/1‑93 (MQ=255)
                                          aaaTCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCg                                               >  1:5521898/1‑93 (MQ=255)
                                          aaaTCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCg                                               >  1:4738410/1‑93 (MQ=255)
                                          aaaTCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTACTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCg                                               >  2:3460725/1‑93 (MQ=255)
                                            aTCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGtt                                             >  2:5688790/1‑93 (MQ=255)
                                             tCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTc                                            >  1:768558/1‑93 (MQ=255)
                                             tCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTc                                            <  2:4612140/93‑1 (MQ=255)
                                             tCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTc                                            >  2:4651650/1‑93 (MQ=255)
                                             tCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTc                                            <  1:3867820/93‑1 (MQ=255)
                                              cTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTCt                                           <  1:6048685/93‑1 (MQ=255)
                                              cTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTCt                                           <  2:2802163/93‑1 (MQ=255)
                                                gAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTCTgc                                         <  1:3783929/93‑1 (MQ=255)
                                                gAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTCTgc                                         <  1:358979/93‑1 (MQ=255)
                                                   aaaaCTTTTTGGATATGGGCGTTGGGGTTTTGCCGCTAATAGTCCTTTTCCTCGCAGCGTACAT‑GGTTTTTTCATTGGCAGGGCTTTCTgcgt                                       <  2:4270345/93‑2 (MQ=255)
                                                        tGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTCTGCGCTGGCTc                                 >  2:5009351/1‑93 (MQ=255)
                                                        tGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTCTGCGCTGGCTc                                 <  1:5936400/93‑1 (MQ=255)
                                                          ttt‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTCTGCGCTGGCTCCg                               >  2:1844975/1‑93 (MQ=255)
                                                           tt‑GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTCTGCGCTGGCTCCGt                              <  2:3624256/93‑1 (MQ=255)
                                                              gATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTCTGCGCTGGCTCCGTgc                            <  2:4787757/93‑1 (MQ=255)
                                                              gATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTCTGCGCTGGCTCCGTgc                            >  1:3702078/1‑93 (MQ=255)
                                                               atatCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTCTGCGCTGGCTCCGTgcg                           >  1:1427176/1‑93 (MQ=255)
                                                                tatCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTCTGCGCTGGCTCCGTgcgc                          <  2:1043544/93‑1 (MQ=255)
                                                                 atCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTCTGCGCTGGCTCCGTGCGCt                         >  2:4063617/1‑93 (MQ=255)
                                                                    ggCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATc                      >  1:4988807/1‑93 (MQ=255)
                                                                     gCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCg                     <  1:2926216/93‑1 (MQ=255)
                                                                       gTTGCGGGCTTGACGCTGATAGTGCCTTTGCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCggg                   >  2:2270066/1‑92 (MQ=255)
                                                                       gTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGt                   <  2:6116683/93‑1 (MQ=255)
                                                                        ttGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTg                  >  2:3365674/1‑93 (MQ=255)
                                                                         tGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGc                 <  1:1224678/93‑1 (MQ=255)
                                                                          gCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGcc                >  2:1200501/1‑93 (MQ=255)
                                                                           cGGGCTTGACGCTGATAGTGCCGTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCt               <  1:3390391/93‑1 (MQ=255)
                                                                            gggCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTg              >  2:3830453/1‑93 (MQ=255)
                                                                            gggCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTg              >  2:2231524/1‑93 (MQ=255)
                                                                             ggCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTgg             <  2:4754495/93‑1 (MQ=255)
                                                                             ggCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTgg             >  2:3602986/1‑93 (MQ=255)
                                                                               cTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGt           <  2:2842056/93‑1 (MQ=255)
                                                                                 tGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTg         >  2:4973209/1‑93 (MQ=255)
                                                                                  gACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTgg        >  2:1969895/1‑93 (MQ=255)
                                                                                   aCGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGt       <  1:1292213/93‑1 (MQ=255)
                                                                                     gCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTaa     >  1:864683/1‑93 (MQ=255)
                                                                                       tGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACa   >  1:643301/1‑93 (MQ=255)
                                                                                        gATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAg  <  1:4364957/93‑1 (MQ=255)
                                                                                        gATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAg  <  1:1138804/93‑1 (MQ=255)
                                                                                           |                                                                                          
GTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTT‑GATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT‑GCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAG  >  NC_000913/4140222‑4140401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAGCTT  >  NC_000913/4140219‑4140404
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CATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATA                                                                                              <  SRR4302533.2947995/94‑1 (MQ=60)
   GTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTG                                                                                           <  SRR4302533.969189/94‑1 (MQ=60)
   GTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTG                                                                                           >  SRR4302533.2256598/1‑94 (MQ=60)
   GTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTG                                                                                           <  SRR4302533.3563473/94‑1 (MQ=60)
   GTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTG                                                                                           >  SRR4302533.5123555/1‑94 (MQ=60)
      GTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAGATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCT                                                                                        <  SRR4302533.2174860/94‑1 (MQ=60)
      GTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCT                                                                                        <  SRR4302533.2287050/94‑1 (MQ=60)
      GTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCT                                                                                        >  SRR4302533.3940038/1‑94 (MQ=60)
      GTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCT                                                                                        >  SRR4302533.5523410/1‑94 (MQ=60)
      GTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCT                                                                                        >  SRR4302533.2610756/1‑94 (MQ=60)
       TATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTT                                                                                       <  SRR4302533.1510658/94‑1 (MQ=60)
        ATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTT                                                                                      >  SRR4302533.2263045/1‑94 (MQ=60)
        ATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTT                                                                                      <  SRR4302533.2505237/94‑1 (MQ=60)
        ATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTT                                                                                      >  SRR4302533.5184742/1‑94 (MQ=60)
          GGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCC                                                                                    >  SRR4302533.3833500/1‑94 (MQ=60)
          GGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCC                                                                                    <  SRR4302533.643045/94‑1 (MQ=60)
           GCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCT                                                                                   <  SRR4302533.4586598/94‑1 (MQ=60)
            CAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTC                                                                                  >  SRR4302533.174446/1‑94 (MQ=60)
            CAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTC                                                                                  <  SRR4302533.3973935/94‑1 (MQ=60)
             AAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCG                                                                                 >  SRR4302533.1692042/1‑94 (MQ=60)
             AAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCG                                                                                 >  SRR4302533.5385704/1‑94 (MQ=60)
             AAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCG                                                                                 <  SRR4302533.580866/94‑1 (MQ=60)
             AAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCG                                                                                 >  SRR4302533.6234424/1‑94 (MQ=60)
               AGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCA                                                                               <  SRR4302533.5342009/94‑1 (MQ=60)
                GGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAG                                                                              <  SRR4302533.3038424/94‑1 (MQ=60)
                    CAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTA                                                                          <  SRR4302533.1983242/94‑1 (MQ=60)
                     AGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCCGCGTAC                                                                         >  SRR4302533.3804674/1‑94 (MQ=60)
                     AGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTAC                                                                         >  SRR4302533.860827/1‑94 (MQ=60)
                      GTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACA                                                                        <  SRR4302533.2776623/94‑1 (MQ=60)
                      GTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACA                                                                        >  SRR4302533.527284/1‑94 (MQ=60)
                      GTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACA                                                                        >  SRR4302533.1675655/1‑94 (MQ=60)
                        GCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATC                                                                      <  SRR4302533.5929916/94‑1 (MQ=60)
                        GCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATC                                                                      <  SRR4302533.830864/94‑1 (MQ=60)
                         CCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCG                                                                     <  SRR4302533.4229308/94‑1 (MQ=60)
                           GGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGT                                                                   >  SRR4302533.4545371/1‑94 (MQ=60)
                           GGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGT                                                                   <  SRR4302533.3381303/94‑1 (MQ=60)
                           GGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGT                                                                   >  SRR4302533.3129001/1‑94 (MQ=60)
                           GGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGT                                                                   >  SRR4302533.3652313/1‑94 (MQ=60)
                           GGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGT                                                                   >  SRR4302533.2673829/1‑94 (MQ=60)
                           GGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGT                                                                   >  SRR4302533.1288300/1‑94 (MQ=60)
                            GATGCCTTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTT                                                                  <  SRR4302533.2316187/94‑1 (MQ=60)
                            GATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTT                                                                  <  SRR4302533.2967243/94‑1 (MQ=60)
                             ATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTA                                                                 >  SRR4302533.2826317/1‑94 (MQ=60)
                              TTCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTAT                                                                <  SRR4302533.3181337/94‑1 (MQ=60)
                              TGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTAT                                                                >  SRR4302533.6345114/1‑94 (MQ=60)
                              TGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTAT                                                                <  SRR4302533.1978729/94‑1 (MQ=60)
                                 CGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCC                                                             >  SRR4302533.206456/1‑94 (MQ=60)
                                  GTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCA                                                            >  SRR4302533.4652030/1‑94 (MQ=60)
                                   TAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAT                                                           <  SRR4302533.3091102/94‑1 (MQ=60)
                                   TAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAT                                                           <  SRR4302533.3115037/94‑1 (MQ=60)
                                   TAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAT                                                           >  SRR4302533.4249417/1‑94 (MQ=60)
                                    AGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGCGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT                                                          >  SRR4302533.3404913/1‑94 (MQ=60)
                                     GCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTG                                                         >  SRR4302533.3064671/1‑94 (MQ=60)
                                     GCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTG                                                         >  SRR4302533.302325/1‑94 (MQ=60)
                                     GCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTG                                                         >  SRR4302533.3017615/1‑94 (MQ=60)
                                     GCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTG                                                         <  SRR4302533.233079/94‑1 (MQ=60)
                                     GCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTG                                                         <  SRR4302533.607015/94‑1 (MQ=60)
                                      CCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGC                                                        >  SRR4302533.5131760/1‑94 (MQ=60)
                                      CCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGC                                                        >  SRR4302533.942771/1‑94 (MQ=60)
                                       CGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCA                                                       <  SRR4302533.1008690/94‑1 (MQ=60)
                                       CGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCA                                                       >  SRR4302533.1210467/1‑94 (MQ=60)
                                        GATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAG                                                      <  SRR4302533.5425775/94‑1 (MQ=60)
                                        GATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAG                                                      <  SRR4302533.3186768/94‑1 (MQ=60)
                                         ATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGA                                                     >  SRR4302533.1947934/1‑94 (MQ=60)
                                         ATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGA                                                     >  SRR4302533.5676377/1‑94 (MQ=60)
                                         ATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGA                                                     >  SRR4302533.4073558/1‑94 (MQ=60)
                                          TCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAG                                                    <  SRR4302533.2460697/94‑1 (MQ=60)
                                          TCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAG                                                    >  SRR4302533.4162234/1‑94 (MQ=60)
                                          TCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGCGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAG                                                    >  SRR4302533.2622426/1‑94 (MQ=60)
                                           CCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGC                                                   >  SRR4302533.3355714/1‑94 (MQ=60)
                                            CAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCG                                                  >  SRR4302533.4767141/1‑94 (MQ=60)
                                            CAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCG                                                  >  SRR4302533.4418063/1‑94 (MQ=60)
                                            CAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCG                                                  >  SRR4302533.5555241/1‑94 (MQ=60)
                                             AAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGT                                                 >  SRR4302533.1714597/1‑94 (MQ=60)
                                               ATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTC                                               >  SRR4302533.908688/1‑94 (MQ=60)
                                               ATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTC                                               >  SRR4302533.2647103/1‑94 (MQ=60)
                                               ATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTC                                               >  SRR4302533.773089/1‑94 (MQ=60)
                                                TCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCT                                              <  SRR4302533.3891431/94‑1 (MQ=60)
                                                TCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCT                                              <  SRR4302533.1261473/94‑1 (MQ=60)
                                                 CTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTG                                             >  SRR4302533.479454/1‑94 (MQ=60)
                                                 CTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTG                                             <  SRR4302533.6085331/94‑1 (MQ=60)
                                                 CTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTG                                             <  SRR4302533.6104931/94‑1 (MQ=60)
                                                 CTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTG                                             <  SRR4302533.2691873/94‑1 (MQ=60)
                                                  TGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGC                                            >  SRR4302533.5230796/1‑94 (MQ=60)
                                                   GAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCG                                           <  SRR4302533.3807099/94‑1 (MQ=60)
                                                   GAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCG                                           <  SRR4302533.361163/94‑1 (MQ=60)
                                                      AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTG                                        >  SRR4302533.5963317/1‑94 (MQ=60)
                                                          CTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTC                                    >  SRR4302533.822877/1‑94 (MQ=60)
                                                           TGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCC                                   <  SRR4302533.5972295/94‑1 (MQ=60)
                                                            GTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCG                                  <  SRR4302533.1310212/94‑1 (MQ=60)
                                                              TTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTG                                >  SRR4302533.3619643/1‑94 (MQ=60)
                                                               TGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGC                               >  SRR4302533.3724809/1‑94 (MQ=60)
                                                               TGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGC                               >  SRR4302533.5907980/1‑94 (MQ=60)
                                                                GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCG                              >  SRR4302533.1435801/1‑94 (MQ=60)
                                                                  TATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCT                            >  SRR4302533.694152/1‑94 (MQ=60)
                                                                  TATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCT                            >  SRR4302533.1871769/1‑94 (MQ=60)
                                                                   ATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTA                           >  SRR4302533.4759387/1‑94 (MQ=60)
                                                                   ATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTA                           >  SRR4302533.198114/1‑94 (MQ=60)
                                                                    TCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTAT                          >  SRR4302533.2520344/1‑94 (MQ=60)
                                                                     CGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATC                         >  SRR4302533.5019052/1‑94 (MQ=60)
                                                                      GGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCG                        <  SRR4302533.504903/94‑1 (MQ=60)
                                                                       GCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGG                       <  SRR4302533.2944121/94‑1 (MQ=60)
                                                                       GCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGG                       <  SRR4302533.2944119/94‑1 (MQ=60)
                                                                        CGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGT                      >  SRR4302533.6060734/1‑94 (MQ=60)
                                                                         GTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTG                     >  SRR4302533.504352/1‑94 (MQ=60)
                                                                           TGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCC                   <  SRR4302533.1232019/94‑1 (MQ=60)
                                                                            GCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCT                  <  SRR4302533.1679624/94‑1 (MQ=60)
                                                                             CGGGCTTGACGCTGATAGTGCCGTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTG                 <  SRR4302533.3411329/94‑1 (MQ=60)
                                                                              GGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGG                >  SRR4302533.5634182/1‑94 (MQ=60)
                                                                               GGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGG               <  SRR4302533.1012407/94‑1 (MQ=60)
                                                                                 CTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTT             >  SRR4302533.4683464/1‑94 (MQ=60)
                                                                                 CTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTCGGTT             >  SRR4302533.3951946/1‑94 (MQ=60)
                                                                                    GACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGT          >  SRR4302533.200840/1‑94 (MQ=60)
                                                                                     ACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTA         <  SRR4302533.1299996/94‑1 (MQ=60)
                                                                                      CGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAA        >  SRR4302533.869748/1‑94 (MQ=60)
                                                                                       GCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAAC       <  SRR4302533.1468708/94‑1 (MQ=60)
                                                                                        CTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACA      >  SRR4302533.647084/1‑94 (MQ=60)
                                                                                        CTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACA      >  SRR4302533.856308/1‑94 (MQ=60)
                                                                                          GATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAGC    <  SRR4302533.4391309/94‑1 (MQ=60)
                                                                                          GATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAGC    <  SRR4302533.1145580/94‑1 (MQ=60)
                                                                                           ATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAGCT   <  SRR4302533.2435832/94‑1 (MQ=60)
                                                                                            TAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAGCTT  >  SRR4302533.4600587/1‑94 (MQ=60)
                                                                                             |                                                                                            
CATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAGCTT  >  NC_000913/4140219‑4140404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑