Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,225,050	A→G	E1215G (GAG→GGG)	metH →	B12‑dependent homocysteine‑N5‑methyltetrahydrofolate transmethylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,225,050	0	A	G	99.7%	1178.3 / ‑6.5	358	E1215G (GAG→GGG)	metH	B12‑dependent homocysteine‑N5‑methyltetrahydrofolate transmethylase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (203/154); minor base C (1/0); total (204/154)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 375 total aligned reads displayed.

CGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGT  >  NC_000913/4224960‑4225139

  gACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGgcgc                                                                                       >  2:1720911/1‑93 (MQ=255)

   acaacaAGTACTCCGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCgct                                                                                      <  2:7411969/93‑1 (MQ=255)

    caacAAGTACTACGCTGAAGCAAAAATTCAGCGCGATCGGCTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCgctg                                                                                     <  2:9743905/90‑1 (MQ=255)

    cAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGTgctg                                                                                     >  1:1530304/1‑93 (MQ=255)

    cAGAAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGTTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCgctg                                                                                     <  2:3714635/93‑1 (MQ=255)

     aGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCgctgg                                                                                    >  1:2423154/1‑93 (MQ=255)

     aGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATCCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCgctgg                                                                                    <  2:8698060/93‑1 (MQ=255)

      gaaaGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCgctggc                                                                                   <  2:8884125/91‑1 (MQ=255)

       caggTACTACGCTGAAGCACAAATGCAGCGCGATCGGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCgctggct                                                                                  <  1:6930343/90‑1 (MQ=255)

       cAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCgctggct                                                                                  >  1:656282/1‑93 (MQ=255)

         aGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGctggctgg                                                                                <  2:7505405/93‑1 (MQ=255)

         aGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGCGCGctggctgg                                                                                >  1:5904029/1‑93 (MQ=255)

           tactacGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGCATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCa                                                                              >  2:8295806/1‑93 (MQ=255)

            attacGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCAc                                                                             <  1:8724914/91‑1 (MQ=255)

            actccGCTGTAGCACAAATTCAGCGCGACCAGGTTGAAGATTATCCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCAc                                                                             <  1:4767456/93‑1 (MQ=255)

             ctacGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCAcc                                                                            <  1:5590317/93‑1 (MQ=255)

             ctacGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATACCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCAcc                                                                            >  2:4397203/1‑93 (MQ=255)

                  cTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATc                                                                       >  2:2557841/1‑93 (MQ=255)

                  cTGTAGCAAAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATc                                                                       <  1:3887905/93‑1 (MQ=255)

                    gTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTg                                                                     <  1:4608604/93‑1 (MQ=255)

                    gTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTg                                                                     >  1:3498969/1‑93 (MQ=255)

                    gTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTg                                                                     >  2:9468851/1‑93 (MQ=255)

                    gTAGCACAAATTCAGCGCGATCAGGTGGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTg                                                                     <  2:7594987/93‑1 (MQ=255)

                      aGCACAAATTCAGCGCGATCAGGTGGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTggg                                                                   <  1:3664315/93‑1 (MQ=255)

                       gCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTgggg                                                                  >  2:1543563/1‑93 (MQ=255)

                       gCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTgggg                                                                  >  2:5392415/1‑93 (MQ=255)

                       gCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGACCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTgggg                                                                  >  2:5262707/1‑93 (MQ=255)

                        cacaAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGt                                                                 >  1:169871/1‑93 (MQ=255)

                          caAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTAt                                                               >  1:5207349/1‑93 (MQ=255)

                          aaaaTTCGGGGCGATCGGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTAt                                                               <  1:8424000/92‑1 (MQ=255)

                           aaaTTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATg                                                              <  2:2935913/93‑1 (MQ=255)

                           aaaTCCGGCGCGCTCAGGTTAAATTTTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATg                                                              <  1:9658968/93‑1 (MQ=255)

                            atttCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGa                                                             <  1:2190531/91‑1 (MQ=255)

                            aaTTCAGCGCGATCAGTTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGa                                                             <  1:5444860/93‑1 (MQ=255)

                            aaTTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGGATGa                                                             >  1:4415261/1‑93 (MQ=255)

                             aTTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGAc                                                            <  1:7957997/93‑1 (MQ=255)

                              ttCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGAcg                                                           >  2:7214826/1‑93 (MQ=255)

                               tCAGCGCGATCAGGTTAAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGAcgc                                                          <  2:6703137/93‑1 (MQ=255)

                                  gcgcGAGCAGTTTGAAGATTATCCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGa                                                       <  1:4827868/93‑1 (MQ=255)

                                      gATCAGTTAAAAGATTACGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGa                                                   <  2:3983632/93‑1 (MQ=255)

                                      gATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGa                                                   >  2:1327583/1‑93 (MQ=255)

                                       aTCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGAt                                                  >  1:8010667/1‑93 (MQ=255)

                                       aTCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGAt                                                  >  2:6463792/1‑93 (MQ=255)

                                        tCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGAtt                                                 >  1:9761826/1‑93 (MQ=255)

                                         cggtttGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTc                                                <  2:5581741/89‑1 (MQ=255)

                                          agtttGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTca                                               <  1:1416144/90‑1 (MQ=255)

                                          aGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTca                                               >  2:2836624/1‑93 (MQ=255)

                                           ggTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTcac                                              >  2:4985099/1‑93 (MQ=255)

                                            gTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTcaca                                             >  1:8215510/1‑93 (MQ=255)

                                            gTTGAAGATTAGGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTcaca                                             <  1:6689426/93‑1 (MQ=255)

                                              tGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACaaa                                           <  2:8014506/93‑1 (MQ=255)

                                               gAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGTCTGATTCACAAAt                                          >  2:5098501/1‑93 (MQ=255)

                                               aaaGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAAt                                          <  1:7319187/92‑1 (MQ=255)

                                                aaGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATc                                         <  1:7696346/93‑1 (MQ=255)

                                                aaGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATc                                         <  2:9885665/93‑1 (MQ=255)

                                                 aGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCCTAAATCt                                        >  2:4206242/1‑93 (MQ=255)

                                                 aGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCt                                        <  1:8352939/93‑1 (MQ=255)

                                                  aaTTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTg                                       <  2:8930140/92‑1 (MQ=255)

                                                     tATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCa                                    >  2:5886139/1‑93 (MQ=255)

                                                      tggCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCAc                                   <  1:4299857/91‑1 (MQ=255)

                                                        gCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCCCtt                                 >  1:4963248/1‑93 (MQ=255)

                                                          ccgccgTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACtttt                               <  1:9982152/93‑1 (MQ=255)

                                                          ccgccgTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACtttt                               >  2:3364391/1‑93 (MQ=255)

                                                            gccgTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTcc                             >  1:4103329/1‑93 (MQ=255)

                                                            gccgTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTcc                             >  1:3150774/1‑93 (MQ=255)

                                                             ccgTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCt                            <  1:7819847/93‑1 (MQ=255)

                                                             ccgTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCt                            >  2:6043446/1‑93 (MQ=255)

                                                              cgTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCtt                           <  1:5730070/93‑1 (MQ=255)

                                                               gTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTa                          >  1:7051629/1‑93 (MQ=255)

                                                                tAAAGGTATGCGCGTTACCGACGTTGGGCGCTGGCTGGCACCGAATCTGGGGGATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTac                         >  1:2007173/1‑93 (MQ=255)

                                                                 aaaGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTaca                        >  1:8887548/1‑93 (MQ=255)

                                                                 aaaGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTaca                        <  1:619719/93‑1 (MQ=255)

                                                                      tATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTaaaaaaaa                   >  2:1067668/1‑93 (MQ=255)

                                                                      tATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACaacaaa                   >  2:5415148/1‑93 (MQ=255)

                                                                      tATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACaacaaa                   <  2:2076975/93‑1 (MQ=255)

                                                                        tGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACaacaaaca                 >  1:9622301/1‑93 (MQ=255)

                                                                        tGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACaacaaaca                 >  2:9293672/1‑93 (MQ=255)

                                                                         gAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAg                >  2:5768276/1‑93 (MQ=255)

                                                                          aGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAgg               <  1:2509173/93‑1 (MQ=255)

                                                                          aGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAgg               <  2:6890359/93‑1 (MQ=255)

                                                                          aGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAgg               <  2:6923245/93‑1 (MQ=255)

                                                                          aGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAgg               <  1:6820326/93‑1 (MQ=255)

                                                                            cGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGgc             <  2:8144693/93‑1 (MQ=255)

                                                                              ttACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGgcgc           >  2:8431833/1‑93 (MQ=255)

                                                                                aCCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTc         >  2:9532154/1‑93 (MQ=255)

                                                                                 ccGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCa        >  1:1183277/1‑93 (MQ=255)

                                                                                  cGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCaa       <  2:7095935/93‑1 (MQ=255)

                                                                                  cGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCaa       >  1:8564220/1‑93 (MQ=255)

                                                                                  cGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCaa       >  1:1021560/1‑93 (MQ=255)

                                                                                  cGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCaa       <  2:2618823/93‑1 (MQ=255)

                                                                                   gAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAAt      >  2:3512721/1‑93 (MQ=255)

                                                                                    aaGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATg     <  1:9232753/93‑1 (MQ=255)

                                                                                    aaGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATg     >  1:2937224/1‑93 (MQ=255)

                                                                                     aGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGa    >  2:4525428/1‑93 (MQ=255)

                                                                                      gTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAg   >  1:6521297/1‑93 (MQ=255)

                                                                                      gTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAg   <  1:2646949/93‑1 (MQ=255)

                                                                                      gTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCGTACAACAAACAGGGCGGTAATTGAg   <  2:3611515/93‑1 (MQ=255)

                                                                                      gTTGGGCGCTGGCTGGCACCGAATCTGGAGTATGACGCAGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAACGAg   <  2:4647911/93‑1 (MQ=255)

                                                                                       ttGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAgg  >  1:8908324/1‑92 (MQ=255)

                                                                                       ttGGGCGCTGGCTGGCACCGAATCGGGGGTATCACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAgc  <  2:9032148/93‑2 (MQ=255)

CGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGT  >  NC_000913/4224960‑4225139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 204 total aligned reads displayed.

ACCCGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCC  >  NC_000913/4224957‑4225142

CCCCGGACAGCAAGTACTACGCTGTAGCAAAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGG                                                                                              <  SRR4302540.2113459/94‑1 (MQ=60)

ACCCGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGG                                                                                              >  SRR4302540.1540872/1‑94 (MQ=60)

 CCCGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGG                                                                                             >  SRR4302540.7888610/1‑94 (MQ=60)

 CCCGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGG                                                                                             >  SRR4302540.1058627/1‑94 (MQ=60)

  ccgaagacCAAGTACTCCTCTGTAGCACAAATCCAGCGCGATCAGTTTAAATATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGC                                                                                            <  SRR4302540.4956062/86‑1 (MQ=60)

  CCGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGC                                                                                            <  SRR4302540.4956063/94‑1 (MQ=60)

   CGGACAGCAGGTACTACGCTGTAGCACAAATTCAGCGCGATCAGTTGGAAGATTATCCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCG                                                                                           <  SRR4302540.2260844/94‑1 (MQ=60)

   CGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCG                                                                                           >  SRR4302540.9766962/1‑94 (MQ=60)

   CGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCG                                                                                           >  SRR4302540.8140248/1‑94 (MQ=60)

   CGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCG                                                                                           <  SRR4302540.6679646/94‑1 (MQ=60)

   CGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCG                                                                                           >  SRR4302540.558988/1‑94 (MQ=60)

   CGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCG                                                                                           >  SRR4302540.9587243/1‑94 (MQ=60)

   CGGACAGCAAGTACTACGCTGTAGCAAAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCG                                                                                           <  SRR4302540.6679647/94‑1 (MQ=60)

     GACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCT                                                                                         <  SRR4302540.859324/94‑1 (MQ=60)

     GACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCT                                                                                         <  SRR4302540.8647847/94‑1 (MQ=60)

     GACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCT                                                                                         >  SRR4302540.8267332/1‑94 (MQ=60)

      ACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGTGCTG                                                                                        >  SRR4302540.1547247/1‑94 (MQ=60)

      ACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGCTGGGCGCTG                                                                                        >  SRR4302540.4080839/1‑94 (MQ=60)

       CAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGG                                                                                       >  SRR4302540.7092150/1‑94 (MQ=60)

       CAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGG                                                                                       >  SRR4302540.2449260/1‑94 (MQ=60)

        AGCAAGTACTACGCTGTAGCACAAATTCAGCGGGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGC                                                                                      <  SRR4302540.2757296/94‑1 (MQ=60)

        AGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGC                                                                                      >  SRR4302540.4181731/1‑94 (MQ=60)

        AGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGC                                                                                      >  SRR4302540.4018106/1‑94 (MQ=60)

         GCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCT                                                                                     >  SRR4302540.663274/1‑94 (MQ=60)

         GCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCT                                                                                     >  SRR4302540.663281/1‑94 (MQ=60)

         ACAAGTACTACGCTGTACAACAATTGCAGCGCGATCAGGTAGAAGATTATTCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCT                                                                                     <  SRR4302540.5418887/94‑1 (MQ=60)

          aaatgactccgtgGTAGCACAAATCCGGCGAGATCAGTTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTG                                                                                    <  SRR4302540.7007464/81‑1 (MQ=60)

          CAGGTACTACGCTGAAGCACAAATGCAGCGCGATCGGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTG                                                                                    <  SRR4302540.7007465/94‑1 (MQ=60)

          CAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTG                                                                                    >  SRR4302540.8990150/1‑94 (MQ=60)

          AAAGTACTACGGTGTAGCAAAAATTCAGCGCGATCAGGTTGAATATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTG                                                                                    <  SRR4302540.8081830/94‑1 (MQ=60)

           AAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGG                                                                                   >  SRR4302540.2551522/1‑94 (MQ=60)

           AAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGCGCGCTGGCTGG                                                                                   >  SRR4302540.5969226/1‑94 (MQ=60)

             GTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCA                                                                                 >  SRR4302540.3670306/1‑94 (MQ=60)

             GTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCA                                                                                 >  SRR4302540.8514258/1‑94 (MQ=60)

             GTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCA                                                                                 <  SRR4302540.9828745/94‑1 (MQ=60)

             ATACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCA                                                                                 >  SRR4302540.9776809/1‑94 (MQ=60)

              TACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCAC                                                                                >  SRR4302540.3672684/1‑94 (MQ=60)

              TACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCAC                                                                                >  SRR4302540.552703/1‑94 (MQ=60)

               actaatgttgagcataattaCAGCGCAATCAGGTTAAATATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACC                                                                               <  SRR4302540.4818370/74‑1 (MQ=60)

               ATTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACC                                                                               <  SRR4302540.8826231/94‑1 (MQ=60)

               ACTCCGCTGTAGCACAAATTCAGCGCGACCAGGTTGAAGATTATCCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACC                                                                               <  SRR4302540.4818368/94‑1 (MQ=60)

               ACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACC                                                                               >  SRR4302540.7228337/1‑94 (MQ=60)

                cgaggcgttagaaaaaatgCAGCGCGATCAGTTTAAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCG                                                                              <  SRR4302540.5738259/75‑1 (MQ=60)

                CTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCG                                                                              >  SRR4302540.7053356/1‑94 (MQ=60)

                CTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGGAAAGGTATGAGCGTTACCGAAGGTGGGCGCTGGCTGGCACCG                                                                              >  SRR4302540.3829753/1‑94 (MQ=60)

                 tcccctgtaacaaaaatgCAGCGCTATCAGGTTGAAAATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGA                                                                             <  SRR4302540.7521081/76‑1 (MQ=60)

                   CGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAAT                                                                           <  SRR4302540.308402/94‑1 (MQ=60)

                   CGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAAT                                                                           >  SRR4302540.4421354/1‑94 (MQ=60)

                   CGCTGTAGCAAAAATTCAGCGCGATCAGTTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAAT                                                                           <  SRR4302540.5616672/94‑1 (MQ=60)

                    GCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATC                                                                          <  SRR4302540.8666705/94‑1 (MQ=60)

                     CTTTATCACAAATTCGGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCT                                                                         <  SRR4302540.3126829/94‑1 (MQ=60)

                     CTGTAGCACAAATACAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCT                                                                         <  SRR4302540.6772106/94‑1 (MQ=60)

                     CTGTAGCAAAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCT                                                                         <  SRR4302540.3930128/94‑1 (MQ=60)

                     CTGAAGCAAAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCT                                                                         <  SRR4302540.8477108/94‑1 (MQ=60)

                      ttaagcaaAAATTCCGCGCGATCAGGTTGAAGATTAGGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTG                                                                        <  SRR4302540.2349639/86‑1 (MQ=60)

                      TGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTG                                                                        >  SRR4302540.1276384/1‑94 (MQ=60)

                      TGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTG                                                                        >  SRR4302540.1276385/1‑94 (MQ=60)

                      TGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTG                                                                        >  SRR4302540.1881873/1‑94 (MQ=60)

                       GTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGG                                                                       <  SRR4302540.4658090/94‑1 (MQ=60)

                        aacacaAAATTCAGCGCGATCAGGTGGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGG                                                                      <  SRR4302540.4051887/88‑1 (MQ=60)

                        TAGCACAAATTCAGCGCGATCAGGTGGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGG                                                                      <  SRR4302540.6946456/94‑1 (MQ=60)

                        GAGCACAAATTCAGCGCGATCGGGTTGAATATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGG                                                                      <  SRR4302540.5743503/94‑1 (MQ=60)

                         AGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGG                                                                     >  SRR4302540.8674555/1‑94 (MQ=60)

                         AGCACAAATTCAGCGCGATCAGGTGGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGG                                                                     <  SRR4302540.3704420/94‑1 (MQ=60)

                          GCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGT                                                                    >  SRR4302540.7142766/1‑94 (MQ=60)

                          GCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGT                                                                    >  SRR4302540.171574/1‑94 (MQ=60)

                            ACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTAT                                                                  >  SRR4302540.5264075/1‑94 (MQ=60)

                            ACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTAT                                                                  >  SRR4302540.5264074/1‑94 (MQ=60)

                            AAAAATTAAGCGGGATCAGTTTAAAGATTATCCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTAT                                                                  <  SRR4302540.7297561/94‑1 (MQ=60)

                             aaaattcgggGCGATCGGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATG                                                                 <  SRR4302540.8521778/84‑1 (MQ=60)

                             AAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATG                                                                 <  SRR4302540.4819796/94‑1 (MQ=60)

                              aaatccggcgcgctcaggttaaattTTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGA                                                                <  SRR4302540.9772667/69‑1 (MQ=60)

                              AAATTCAGCGCGATGAGGTTGAAGATTATCCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGA                                                                <  SRR4302540.2847204/94‑1 (MQ=60)

                              AAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGA                                                                >  SRR4302540.2473353/1‑94 (MQ=60)

                              AAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGGATGA                                                                >  SRR4302540.4463110/1‑94 (MQ=60)

                               aattcgtcggtgccaggtggagtattagGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGAC                                                               <  SRR4302540.5250605/66‑1 (MQ=60)

                               ATTTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGAC                                                               <  SRR4302540.2214181/94‑1 (MQ=60)

                               AATTCGGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGAC                                                               <  SRR4302540.4219250/94‑1 (MQ=60)

                               AATTCAGCGCGATCAGTTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGAC                                                               <  SRR4302540.5504778/94‑1 (MQ=60)

                               AATTCAGCGCGATCAGGTTGAAGATTATTCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGAC                                                               <  SRR4302540.450150/94‑1 (MQ=60)

                                ATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACG                                                              <  SRR4302540.8050754/94‑1 (MQ=60)

                                ATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACG                                                              >  SRR4302540.2061770/1‑94 (MQ=60)

                                    gcgGCGATCGGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGA                                                          <  SRR4302540.3811872/91‑1 (MQ=60)

                                     GCGCGAGCAGTTTGAAGATTATCCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGAC                                                         <  SRR4302540.4879567/94‑1 (MQ=60)

                                      CGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACT                                                        >  SRR4302540.8269698/1‑94 (MQ=60)

                                       GCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTG                                                       >  SRR4302540.9488287/1‑94 (MQ=60)

                                       GCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTG                                                       >  SRR4302540.9488283/1‑94 (MQ=60)

                                       GCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTG                                                       >  SRR4302540.3443061/1‑94 (MQ=60)

                                         gaataggttaaaaaaTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGAT                                                     <  SRR4302540.7798661/79‑1 (MQ=60)

                                         GATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGAT                                                     >  SRR4302540.8993308/1‑94 (MQ=60)

                                         GATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGAT                                                     >  SRR4302540.6555917/1‑94 (MQ=60)

                                         GATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGAT                                                     >  SRR4302540.8103858/1‑94 (MQ=60)

                                          ATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATT                                                    >  SRR4302540.9876323/1‑94 (MQ=60)

                                          ATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATT                                                    >  SRR4302540.8688840/1‑94 (MQ=60)

                                           TCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTC                                                   <  SRR4302540.8869769/94‑1 (MQ=60)

                                            CAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCA                                                  <  SRR4302540.1307807/94‑1 (MQ=60)

                                            CAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCA                                                  >  SRR4302540.8531637/1‑94 (MQ=60)

                                            CAGGTTAAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCA                                                  <  SRR4302540.4512098/94‑1 (MQ=60)

                                             AGTTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCAC                                                 <  SRR4302540.1431432/94‑1 (MQ=60)

                                             AGGTTAAAGATTAGCCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCAC                                                 <  SRR4302540.2560069/94‑1 (MQ=60)

                                              GGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACA                                                >  SRR4302540.8310998/1‑94 (MQ=60)

                                              GGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACA                                                >  SRR4302540.1993530/1‑94 (MQ=60)

                                              GGTGGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACA                                                <  SRR4302540.2310275/94‑1 (MQ=60)

                                               GTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAA                                               <  SRR4302540.8435930/94‑1 (MQ=60)

                                               GTTGAAGATTAGGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAA                                               <  SRR4302540.6763285/94‑1 (MQ=60)

                                                TTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAA                                              >  SRR4302540.4816659/1‑94 (MQ=60)

                                                TTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAA                                              >  SRR4302540.9754648/1‑94 (MQ=60)

                                                 TGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGTTATGACGCGGACTGATTCACAAAT                                             >  SRR4302540.5754898/1‑94 (MQ=60)

                                                 TGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAAT                                             >  SRR4302540.5047395/1‑94 (MQ=60)

                                                  GAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATC                                            >  SRR4302540.3121986/1‑94 (MQ=60)

                                                  GAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATC                                            >  SRR4302540.4411136/1‑94 (MQ=60)

                                                  GAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATC                                            <  SRR4302540.4241861/94‑1 (MQ=60)

                                                  AAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATC                                            <  SRR4302540.7400934/94‑1 (MQ=60)

                                                   AAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCT                                           <  SRR4302540.7784070/94‑1 (MQ=60)

                                                    AGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTG                                          >  SRR4302540.1193251/1‑94 (MQ=60)

                                                    AGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTG                                          <  SRR4302540.8449883/94‑1 (MQ=60)

                                                     GATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGT                                         >  SRR4302540.5855030/1‑94 (MQ=60)

                                                      ATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTC                                        >  SRR4302540.6994834/1‑94 (MQ=60)

                                                      ATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTC                                        >  SRR4302540.6880866/1‑94 (MQ=60)

                                                       TTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCA                                       >  SRR4302540.898313/1‑94 (MQ=60)

                                                       TTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCA                                       <  SRR4302540.5266775/94‑1 (MQ=60)

                                                       TTAGGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCA                                       <  SRR4302540.5927885/94‑1 (MQ=60)

                                                        TATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCAC                                      >  SRR4302540.9214117/1‑94 (MQ=60)

                                                        TATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCAC                                      >  SRR4302540.1660842/1‑94 (MQ=60)

                                                         tgGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACT                                     <  SRR4302540.4346661/92‑1 (MQ=60)

                                                          TGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCCCTT                                    >  SRR4302540.5016925/1‑94 (MQ=60)

                                                            CCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTT                                  >  SRR4302540.4515936/1‑94 (MQ=60)

                                                             CCGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTC                                 <  SRR4302540.10098545/94‑1 (MQ=60)

                                                              CGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCC                                >  SRR4302540.4147598/1‑94 (MQ=60)

                                                              CGCCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCC                                >  SRR4302540.3185083/1‑94 (MQ=60)

                                                                CCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTT                              <  SRR4302540.1518477/94‑1 (MQ=60)

                                                                CCGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTT                              <  SRR4302540.7911234/94‑1 (MQ=60)

                                                                 CGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTA                             >  SRR4302540.6702101/1‑94 (MQ=60)

                                                                 CGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTA                             <  SRR4302540.5793170/94‑1 (MQ=60)

                                                                 CGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTA                             <  SRR4302540.1518929/94‑1 (MQ=60)

                                                                 CGTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTA                             >  SRR4302540.7130052/1‑94 (MQ=60)

                                                                  GTAAAGGTATGCGCGTTACCGACGTTGGGCGCTGGCTGGCACCGAATCTGGGGGATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTAC                            >  SRR4302540.2028887/1‑94 (MQ=60)

                                                                  GTAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTAC                            >  SRR4302540.6608596/1‑94 (MQ=60)

                                                                   TAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACA                           >  SRR4302540.8990438/1‑94 (MQ=60)

                                                                   TAAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACA                           <  SRR4302540.4778884/94‑1 (MQ=60)

                                                                    AAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAA                          <  SRR4302540.6216297/94‑1 (MQ=60)

                                                                    AAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAA                          <  SRR4302540.626317/94‑1 (MQ=60)

                                                                    AAAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAA                          <  SRR4302540.1563702/94‑1 (MQ=60)

                                                                     AAGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAAC                         >  SRR4302540.1631422/1‑94 (MQ=60)

                                                                      AGGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACA                        <  SRR4302540.2134702/94‑1 (MQ=60)

                                                                       AGTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAA                       <  SRR4302540.3975974/94‑1 (MQ=60)

                                                                        GTATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAA                      >  SRR4302540.2518443/1‑94 (MQ=60)

                                                                         TATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAAC                     >  SRR4302540.6403347/1‑94 (MQ=60)

                                                                         TATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAAC                     >  SRR4302540.6403346/1‑94 (MQ=60)

                                                                         TATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAAC                     <  SRR4302540.5113112/94‑1 (MQ=60)

                                                                         TATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAAC                     >  SRR4302540.9895566/1‑94 (MQ=60)

                                                                          ATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACA                    >  SRR4302540.6096046/1‑94 (MQ=60)

                                                                          ATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACA                    <  SRR4302540.2975901/94‑1 (MQ=60)

                                                                          ATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACA                    <  SRR4302540.5239765/94‑1 (MQ=60)

                                                                          ATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACA                    >  SRR4302540.8951544/1‑94 (MQ=60)

                                                                          ATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACA                    >  SRR4302540.7882710/1‑94 (MQ=60)

                                                                          ATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACA                    >  SRR4302540.7882708/1‑94 (MQ=60)

                                                                          ATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACA                    >  SRR4302540.9735718/1‑94 (MQ=60)

                                                                          ATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTCTTCCTTACAACAAACA                    >  SRR4302540.2509860/1‑94 (MQ=60)

                                                                          ATGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGAGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACA                    <  SRR4302540.743837/94‑1 (MQ=60)

                                                                           TGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAG                   <  SRR4302540.8312915/94‑1 (MQ=60)

                                                                           TGAGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAG                   <  SRR4302540.5884162/94‑1 (MQ=60)

                                                                             AGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGG                 >  SRR4302540.1108848/1‑94 (MQ=60)

                                                                             AGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGG                 <  SRR4302540.2536272/94‑1 (MQ=60)

                                                                             AGCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGG                 <  SRR4302540.6895828/94‑1 (MQ=60)

                                                                              GCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGC                >  SRR4302540.9743493/1‑94 (MQ=60)

                                                                              GCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGC                <  SRR4302540.4821285/94‑1 (MQ=60)

                                                                              GCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGC                <  SRR4302540.490414/94‑1 (MQ=60)

                                                                              GCGTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGCGC                >  SRR4302540.4752195/1‑94 (MQ=60)

                                                                                GTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGC              >  SRR4302540.911012/1‑94 (MQ=60)

                                                                                GTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGC              >  SRR4302540.6981522/1‑94 (MQ=60)

                                                                                GTTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGC              >  SRR4302540.6009953/1‑94 (MQ=60)

                                                                                 TTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGgcct             >  SRR4302540.3857253/1‑90 (MQ=60)

                                                                                 TTACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCT             <  SRR4302540.4891811/94‑1 (MQ=60)

                                                                                  TACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTC            >  SRR4302540.1440809/1‑94 (MQ=60)

                                                                                   ACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCA           <  SRR4302540.76289/94‑1 (MQ=60)

                                                                                   ACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCA           >  SRR4302540.1195493/1‑94 (MQ=60)

                                                                                   ACCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCA           >  SRR4302540.9509842/1‑94 (MQ=60)

                                                                                    CCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAA          >  SRR4302540.1031926/1‑94 (MQ=60)

                                                                                    CCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAA          >  SRR4302540.8663677/1‑94 (MQ=60)

                                                                                    CCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAA          >  SRR4302540.3244418/1‑94 (MQ=60)

                                                                                    CCGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAA          >  SRR4302540.807710/1‑94 (MQ=60)

                                                                                    CCGAAGTTGGGCGCTGGCCGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAA          >  SRR4302540.807709/1‑94 (MQ=60)

                                                                                     CGAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAAT         >  SRR4302540.6867587/1‑94 (MQ=60)

                                                                                      GAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATG        >  SRR4302540.2969461/1‑94 (MQ=60)

                                                                                      GAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATG        <  SRR4302540.215711/94‑1 (MQ=60)

                                                                                      GAAGTTGGGCGCTGGCTGGCACCGAATCTGGGGGATGACGAGGACTGATTCACAAATCTGTCACTTTTCCTTCCAACAAAaagcgaggaccaag        >  SRR4302540.7437543/1‑80 (MQ=60)

                                                                                       AAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGA       <  SRR4302540.9343405/94‑1 (MQ=60)

                                                                                       AAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGA       <  SRR4302540.8353099/94‑1 (MQ=60)

                                                                                       AAGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGA       <  SRR4302540.9896427/94‑1 (MQ=60)

                                                                                        AGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAG      <  SRR4302540.9318344/94‑1 (MQ=60)

                                                                                        AGTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAG      >  SRR4302540.6593730/1‑94 (MQ=60)

                                                                                        AGTTGGGCGCTGGCTGGAGACGAATCTGGGGTATGACGCGGACTTATTCACAAATCTGTCACTTTTCCTTACACCAAACAGGGCGCTCAATGAG      <  SRR4302540.4820306/94‑1 (MQ=60)

                                                                                         GTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGgctcataaag     >  SRR4302540.8990172/1‑84 (MQ=60)

                                                                                         GTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCcatgaag     >  SRR4302540.79410/1‑87 (MQ=60)

                                                                                         GTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGG     >  SRR4302540.9011477/1‑94 (MQ=60)

                                                                                         GTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGC     >  SRR4302540.3278953/1‑94 (MQ=60)

                                                                                         GTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGC     <  SRR4302540.2675662/94‑1 (MQ=60)

                                                                                         GTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCCCTCAATGAGC     <  SRR4302540.2675663/94‑1 (MQ=60)

                                                                                            GGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCC  <  SRR4302540.4152417/94‑1 (MQ=60)

ACCCGGACAGCAAGTACTACGCTGTAGCACAAATTCAGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCC  >  NC_000913/4224957‑4225142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`