Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,311,496 +C coding (301/408 nt) phnQ ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,311,4951.C99.1% 1420.2 / 2.7 442coding (302/408 nt)phnQhypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (1/3);  new base C (201/237);  total (202/240)
Fisher's exact test for biased strand distribution p-value = 6.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 487 total aligned reads displayed.

GAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑A‑CGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGCG  >  NC_000913/4311403‑4311582
                                                                                                |                                                                                       
gAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑a                                                                                          >  2:4707929/1‑93 (MQ=255)
 aGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑A‑c                                                                                        <  2:2084369/93‑1 (MQ=255)
    aaTGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑A‑ccgt                                                                                     <  2:9818465/93‑4 (MQ=255)
       gATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTcc                                                                                   >  2:3359537/1‑93 (MQ=255)
       gATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTcc                                                                                   <  1:7496879/93‑1 (MQ=255)
       gATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTcc                                                                                   <  1:4153767/93‑1 (MQ=255)
        aTTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCg                                                                                  >  1:9345944/1‑93 (MQ=255)
         ttAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCgg                                                                                 >  2:2259043/1‑93 (MQ=255)
          tAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGc                                                                                >  2:1776500/1‑93 (MQ=255)
          tAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGc                                                                                >  2:9275907/1‑93 (MQ=255)
            aTGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAAAGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGccc                                                                              >  2:3195106/1‑93 (MQ=255)
            aTGTGGATAGAGTGTCTTTTTGCGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACTCTGCTCACGCTGT‑CTC‑C‑CGTTCCGGccc                                                                              >  1:1241772/1‑93 (MQ=255)
             tgtgGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCa                                                                             <  1:8414109/93‑1 (MQ=255)
                ggATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGc                                                                          <  1:5564281/93‑1 (MQ=255)
                  aTAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCATGCTGT‑C‑C‑ACCGTTTCGGCCCAGGCCg                                                                        >  2:1918570/1‑93 (MQ=255)
                  aTAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCg                                                                        >  1:3217922/1‑93 (MQ=255)
                  aTAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCg                                                                        >  2:7379710/1‑93 (MQ=255)
                  aTAGAGTTACTTTTAGAGGTTGCGTAATTATACATTTGCCGCTATTTCTTTTATTCGCCCTGCCCACGGTTTGC‑G‑C‑CGTTCAGGCCCAGGctg                                                                        <  2:7655260/93‑3 (MQ=255)
                   tAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGATCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGa                                                                       >  1:4190505/1‑93 (MQ=255)
                     gagTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGata                                                                     <  2:9028839/93‑1 (MQ=255)
                       gTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGAtatt                                                                   >  2:7053886/1‑93 (MQ=255)
                        tttCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGAtatta                                                                  >  1:8165479/1‑93 (MQ=255)
                        tttCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGAtatta                                                                  >  2:2770786/1‑93 (MQ=255)
                        tttCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGAtatta                                                                  >  2:3049331/1‑93 (MQ=255)
                         ttCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGAtattat                                                                 <  2:5451635/93‑1 (MQ=255)
                          tCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATc                                                                >  2:2455351/1‑93 (MQ=255)
                             ttttGAGGTTGGCTAATGAAACTCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATcgac                                                             >  1:1815718/1‑93 (MQ=255)
                             ttttGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATcgac                                                             >  1:9085468/1‑93 (MQ=255)
                               ttGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATcgacga                                                           <  1:4929468/93‑1 (MQ=255)
                               ttGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATcgacga                                                           >  2:9486568/1‑93 (MQ=255)
                               ttGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATcgacga                                                           <  2:8522882/93‑1 (MQ=255)
                                 gAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCTCCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGaca                                                         <  2:793682/93‑1 (MQ=255)
                                 gAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGaca                                                         <  1:6245000/93‑1 (MQ=255)
                                  aGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGacac                                                        >  1:5750321/1‑93 (MQ=255)
                                   ggTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACAcc                                                       >  1:3077086/1‑93 (MQ=255)
                                    gTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCa                                                      >  2:4484719/1‑93 (MQ=255)
                                     ttGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCAt                                                     <  2:5019945/93‑1 (MQ=255)
                                     ttGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCAt                                                     <  2:5972434/93‑1 (MQ=255)
                                      tGGCTAATGAAACGCTTTCCGCTATTTCTTTTAGTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCAtt                                                    <  1:9713207/93‑1 (MQ=255)
                                       ggCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTg                                                   <  1:2657241/93‑1 (MQ=255)
                                          tAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCAGGGCCAGGACGATATTATCGACCACACAATTGGCa                                                >  2:7508739/1‑93 (MQ=255)
                                           aaTGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCaa                                               >  2:1685981/1‑93 (MQ=255)
                                           aaTGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCaa                                               >  2:8158901/1‑93 (MQ=255)
                                           aaTGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCaa                                               >  1:2298435/1‑93 (MQ=255)
                                             tGAAACGCTTTCCCCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAata                                             <  1:5898846/93‑1 (MQ=255)
                                                 aCGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTca                                         <  2:4880943/93‑1 (MQ=255)
                                                  cGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTcag                                        >  1:7612697/1‑93 (MQ=255)
                                                   gCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTcagc                                       <  1:9578822/93‑1 (MQ=255)
                                                   gCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTcagc                                       <  2:3746999/93‑1 (MQ=255)
                                                    cTTTCCGTTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTcagca                                      <  1:9464428/93‑1 (MQ=255)
                                                    cTTTCCGCTATTTCTTTTATTCCCCCTACTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTcagca                                      <  2:1497048/93‑1 (MQ=255)
                                                    cTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTcagca                                      <  1:3886561/93‑1 (MQ=255)
                                                    cTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTcagca                                      <  2:4370690/93‑1 (MQ=255)
                                                    cTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTcagca                                      <  1:6703672/93‑1 (MQ=255)
                                                     tttCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTcagcag                                     >  1:2934528/1‑93 (MQ=255)
                                                     tttCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTcagcag                                     >  2:8121257/1‑93 (MQ=255)
                                                     tttCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTACCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTcagcag                                     <  2:9597284/93‑1 (MQ=255)
                                                      ttCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTGAGCAgg                                    <  1:1670858/93‑1 (MQ=255)
                                                       tCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTTGCAATATTCAGCAGGc                                   >  2:57194/1‑93 (MQ=255)
                                                          gCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGAt                                >  1:7239431/1‑93 (MQ=255)
                                                           cTATTTCTTCTATTCACCCTGCCCACCCTTTCC‑C‑C‑CGTTCCGGCCCAGGCCGATATTATCGACGACCCCATTGTCAATATTCAGCAGGCGAtt                               <  1:1104569/93‑1 (MQ=255)
                                                            tATTTCTTTTATTCCCCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTa                              <  2:3282885/93‑1 (MQ=255)
                                                            tATTTCTTTTATTCACCCTGCTCACGCTTC‑C‑C‑ACCGTTCCGTCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTa                              <  1:1838098/93‑1 (MQ=255)
                                                            tATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTa                              <  2:1571538/93‑1 (MQ=255)
                                                            tATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTa                              <  1:4232819/93‑1 (MQ=255)
                                                            tATTTCTTTTATTCACCCTGCTCACGCCGT‑C‑CTC‑CGTTCCGGCCCAGGCCGATATTATCGACGGCACCATTGGCAATATTCAGCAGGCGATTa                              <  2:6674631/93‑1 (MQ=255)
                                                             aTTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTaa                             <  1:1541985/93‑1 (MQ=255)
                                                             aTTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTaa                             <  1:3694356/93‑1 (MQ=255)
                                                              tttCTTTTATTCACCCTGCTCACTCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAAt                            <  2:2639211/93‑1 (MQ=255)
                                                              tttCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAAt                            >  2:781504/1‑93 (MQ=255)
                                                              tttCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAAt                            <  1:9904686/93‑1 (MQ=255)
                                                              tttCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAAt                            <  1:8719165/93‑1 (MQ=255)
                                                               ttCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATg                           >  1:6481451/1‑93 (MQ=255)
                                                                tCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGa                          >  1:4386667/1‑93 (MQ=255)
                                                                  ttttATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACg                        >  2:1182799/1‑93 (MQ=255)
                                                                    ttATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCt                      <  1:6113306/93‑1 (MQ=255)
                                                                    ttATTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCt                      <  1:4500758/93‑1 (MQ=255)
                                                                      aTTCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTta                    >  1:5081886/1‑93 (MQ=255)
                                                                       ttCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTtat                   <  2:8383432/93‑1 (MQ=255)
                                                                       ttCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTtat                   <  1:7957209/93‑1 (MQ=255)
                                                                        tcgcccTGCTCCCGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTtata                  <  2:6910825/90‑1 (MQ=255)
                                                                        tCACCCTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTtata                  <  1:5231644/93‑1 (MQ=255)
                                                                          ccccTGCTAACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAAc                <  1:1948375/92‑1 (MQ=255)
                                                                            ccTGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAAccc              >  2:6791474/1‑93 (MQ=255)
                                                                              tGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTg            >  2:5473596/1‑93 (MQ=255)
                                                                              tGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTg            >  1:4687399/1‑93 (MQ=255)
                                                                              tGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTg            <  1:7009657/93‑1 (MQ=255)
                                                                              tGCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTg            <  2:3396688/93‑1 (MQ=255)
                                                                               gCTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGa           <  1:10018939/93‑1 (MQ=255)
                                                                                cTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGAc          <  1:3307158/93‑1 (MQ=255)
                                                                                cTCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGAc          >  2:5242572/1‑93 (MQ=255)
                                                                                 tCACGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGAcc         >  2:3584130/1‑93 (MQ=255)
                                                                                   aCGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGt       >  2:8873994/1‑93 (MQ=255)
                                                                                   aCGCTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGt       >  2:4092/1‑93 (MQ=255)
                                                                                    cGCTGT‑C‑C‑ACCGTGCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTg      <  1:4419895/93‑1 (MQ=255)
                                                                                      cTGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTggg    <  2:7110040/93‑1 (MQ=255)
                                                                                       tGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGc   >  1:2132539/1‑93 (MQ=255)
                                                                                       tGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGc   <  1:1497240/93‑1 (MQ=255)
                                                                                       tGT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGc   <  2:3480364/93‑1 (MQ=255)
                                                                                        gT‑C‑C‑ACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGCg  >  2:4248818/1‑93 (MQ=255)
                                                                                                |                                                                                       
GAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGT‑C‑C‑A‑CGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGCG  >  NC_000913/4311403‑4311582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 256 total aligned reads displayed.
AGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑A‑CG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGCGAGACTAT  >  NC_000913/4311404‑4311589
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AGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑A‑CC                                                                                               >  SRR4302540.1853523/1‑94 (MQ=60)
AGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑A‑CC                                                                                               >  SRR4302540.8815241/1‑94 (MQ=60)
 GTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑A‑Ccg                                                                                              >  SRR4302540.3021887/1‑92 (MQ=60)
  TAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑A‑Ccgt                                                                                             <  SRR4302540.5421430/94‑4 (MQ=60)
  TAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑A‑Ccgt                                                                                             <  SRR4302540.3825796/94‑4 (MQ=60)
  TAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑A‑Ccgt                                                                                             >  SRR4302540.5908861/1‑91 (MQ=60)
    ATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTC                                                                                           >  SRR4302540.6967827/1‑94 (MQ=60)
    ATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTC                                                                                           >  SRR4302540.7999976/1‑94 (MQ=60)
    ATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTC                                                                                           >  SRR4302540.3726114/1‑94 (MQ=60)
     TGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCC                                                                                          <  SRR4302540.9885195/94‑1 (MQ=60)
     TGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCC                                                                                          <  SRR4302540.6515317/94‑1 (MQ=60)
     TGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCC                                                                                          <  SRR4302540.6515315/94‑1 (MQ=60)
      GATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCG                                                                                         <  SRR4302540.7580627/94‑1 (MQ=60)
      GATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCG                                                                                         <  SRR4302540.4845447/94‑1 (MQ=60)
      GATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCG                                                                                         >  SRR4302540.9457281/1‑94 (MQ=60)
      GATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCG                                                                                         <  SRR4302540.4198761/94‑1 (MQ=60)
       ATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGG                                                                                        >  SRR4302540.7690765/1‑94 (MQ=60)
        TTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGC                                                                                       >  SRR4302540.503146/1‑94 (MQ=60)
          AATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCC                                                                                     >  SRR4302540.4378042/1‑94 (MQ=60)
          AATGTGGATAGAGTGTCTTTTTGCGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACTCTGCTCACGCTGTCTC‑C‑CG‑TTCCGGCCC                                                                                     >  SRR4302540.1254443/1‑94 (MQ=60)
           ATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTGCGGCCCA                                                                                    >  SRR4302540.1835608/1‑94 (MQ=60)
           ATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTGCGGCCCA                                                                                    >  SRR4302540.1835609/1‑94 (MQ=60)
           ATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCA                                                                                    <  SRR4302540.9110136/94‑1 (MQ=60)
           ATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCA                                                                                    >  SRR4302540.328708/1‑94 (MQ=60)
            TGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTCTTTATTTTATTCACCCTGCTCACGCTGTC‑CTC‑CG‑TTCCGGCCCAG                                                                                   >  SRR4302540.4271777/1‑94 (MQ=60)
            TGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAG                                                                                   >  SRR4302540.4271779/1‑94 (MQ=60)
            TGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAG                                                                                   <  SRR4302540.9416441/94‑1 (MQ=60)
            TGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAG                                                                                   <  SRR4302540.8511771/94‑1 (MQ=60)
            TGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAG                                                                                   <  SRR4302540.1532956/94‑1 (MQ=60)
              TGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGC                                                                                 >  SRR4302540.5990825/1‑94 (MQ=60)
              TGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGC                                                                                 >  SRR4302540.8887716/1‑94 (MQ=60)
               GGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCC                                                                                <  SRR4302540.5625554/94‑1 (MQ=60)
               GGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCC                                                                                >  SRR4302540.4217264/1‑94 (MQ=60)
                GATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCG                                                                               <  SRR4302540.1857845/94‑1 (MQ=60)
                GATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCG                                                                               >  SRR4302540.3252873/1‑94 (MQ=60)
                 ATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGATCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGA                                                                              >  SRR4302540.4236074/1‑94 (MQ=60)
                  TAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGAT                                                                             >  SRR4302540.636096/1‑94 (MQ=60)
                   AGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATA                                                                            <  SRR4302540.6513957/94‑1 (MQ=60)
                   AAAGTTTCTTTTTGAGATTGCCTAATGAAACACTTTCCTCTATTTCTTTTATTCACCCTGCTCCCGCCGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATA                                                                            <  SRR4302540.998968/94‑1 (MQ=60)
                    GAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATAT                                                                           <  SRR4302540.8792078/94‑1 (MQ=60)
                    GAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATAT                                                                           >  SRR4302540.5021051/1‑94 (MQ=60)
                     AGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGAC‑C‑ACCG‑TTCCGGCCCAGGCCGATATT                                                                          >  SRR4302540.1209081/1‑94 (MQ=60)
                      GTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTCTTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTA                                                                         >  SRR4302540.1953430/1‑94 (MQ=60)
                      GTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTA                                                                         >  SRR4302540.8260296/1‑94 (MQ=60)
                      GTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTA                                                                         >  SRR4302540.6085721/1‑94 (MQ=60)
                       TTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTAT                                                                        >  SRR4302540.1370206/1‑94 (MQ=60)
                       TTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTAT                                                                        <  SRR4302540.780856/94‑1 (MQ=60)
                        tttttgtgtgggttagttaataaatctcttccctttttttttttttttccCCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATC                                                                       <  SRR4302540.9288847/44‑1 (MQ=60)
                          CTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGA                                                                     >  SRR4302540.5964842/1‑94 (MQ=60)
                           TTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGAC                                                                    <  SRR4302540.434803/94‑1 (MQ=60)
                           TTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGAC                                                                    <  SRR4302540.10010010/94‑1 (MQ=60)
                           TTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGAC                                                                    >  SRR4302540.9194623/1‑94 (MQ=60)
                           TTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGAC                                                                    <  SRR4302540.1465300/94‑1 (MQ=60)
                           TTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGAC                                                                    <  SRR4302540.1715218/94‑1 (MQ=60)
                           TTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGAC                                                                    >  SRR4302540.1743415/1‑94 (MQ=60)
                              TTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGAC                                                                 <  SRR4302540.5082727/94‑1 (MQ=60)
                              TTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGAC                                                                 <  SRR4302540.4982600/94‑1 (MQ=60)
                              TTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGAC                                                                 <  SRR4302540.6680288/94‑1 (MQ=60)
                               TGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACC                                                                >  SRR4302540.1792888/1‑94 (MQ=60)
                               TGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACA                                                                >  SRR4302540.5389569/1‑94 (MQ=60)
                               TGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACA                                                                <  SRR4302540.2101722/94‑1 (MQ=60)
                                GAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACAC                                                               >  SRR4302540.5813789/1‑94 (MQ=60)
                                 AGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACC                                                              >  SRR4302540.3110844/1‑94 (MQ=60)
                                  GGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCA                                                             >  SRR4302540.969331/1‑94 (MQ=60)
                                   GTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCAT                                                            >  SRR4302540.3655519/1‑94 (MQ=60)
                                   GTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCAT                                                            <  SRR4302540.1574379/94‑1 (MQ=60)
                                   GTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCACGCCGATATTATCGACGACACCAT                                                            >  SRR4302540.6189439/1‑94 (MQ=60)
                                    TTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATT                                                           >  SRR4302540.153068/1‑94 (MQ=60)
                                    TTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATT                                                           <  SRR4302540.8489661/94‑1 (MQ=60)
                                    TTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATT                                                           >  SRR4302540.4730352/1‑94 (MQ=60)
                                     TGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTG                                                          >  SRR4302540.221367/1‑94 (MQ=60)
                                     TGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTG                                                          <  SRR4302540.2057660/94‑1 (MQ=60)
                                     TGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATCATCGACGACACCATTG                                                          <  SRR4302540.9911619/94‑1 (MQ=60)
                                     TGGCTAATGAAACGCTTTCCGCTATTTCTTTTAGTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTG                                                          <  SRR4302540.9827351/94‑1 (MQ=60)
                                      GGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGG                                                         <  SRR4302540.2686079/94‑1 (MQ=60)
                                      GGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGG                                                         <  SRR4302540.3181873/94‑1 (MQ=60)
                                        CTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCA                                                       >  SRR4302540.1474955/1‑94 (MQ=60)
                                        CTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCA                                                       <  SRR4302540.8051754/94‑1 (MQ=60)
                                         TAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAA                                                      >  SRR4302540.2323166/1‑94 (MQ=60)
                                         TAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑A‑A‑CGTTTCCGGCCCAGGCCGtaattttcgacgcccccatgggcat                                                      >  SRR4302540.1912239/1‑69 (MQ=60)
                                          AATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAAT                                                     >  SRR4302540.6800143/1‑94 (MQ=60)
                                            TGAAACGCTTTCCCCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATAT                                                   <  SRR4302540.5963992/94‑1 (MQ=60)
                                             GAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATT                                                  >  SRR4302540.2362992/1‑94 (MQ=60)
                                             GAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATT                                                  <  SRR4302540.2161200/94‑1 (MQ=60)
                                              AAACGCTTTCCGCTATTTCTTTTATTCCCCCTGCTCGCGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTC                                                 <  SRR4302540.4235080/94‑1 (MQ=60)
                                              AAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTC                                                 >  SRR4302540.9499159/1‑94 (MQ=60)
                                              AAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTC                                                 <  SRR4302540.9521341/94‑1 (MQ=60)
                                              AAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTC                                                 <  SRR4302540.684381/94‑1 (MQ=60)
                                              AAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTC                                                 <  SRR4302540.9814143/94‑1 (MQ=60)
                                                ACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAG                                               <  SRR4302540.7506265/94‑1 (MQ=60)
                                                ACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAG                                               <  SRR4302540.9678010/94‑1 (MQ=60)
                                                ACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAG                                               >  SRR4302540.7698259/1‑94 (MQ=60)
                                                ACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAG                                               <  SRR4302540.8600993/94‑1 (MQ=60)
                                                ACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAG                                               <  SRR4302540.6365556/94‑1 (MQ=60)
                                                ACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAG                                               <  SRR4302540.1114172/94‑1 (MQ=60)
                                                  GCTTTCCGCTATTTCTTTTATTCCCCGTGCTCACACTGCC‑C‑ACCG‑GTCCGGCCCAGCCCGTTATTATCGCCGCCACCATTGGCAATATTCAGCA                                             <  SRR4302540.3264347/94‑1 (MQ=36)
                                                  GCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGTTATTATCGACGACACCATTGGCAATATTCAGCA                                             <  SRR4302540.3264348/94‑1 (MQ=60)
                                                  GCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCA                                             >  SRR4302540.2561095/1‑94 (MQ=60)
                                                  GCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCA                                             <  SRR4302540.121617/94‑1 (MQ=60)
                                                  GCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCA                                             <  SRR4302540.9691944/94‑1 (MQ=60)
                                                   CTTTCCGTTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAG                                            <  SRR4302540.9576710/94‑1 (MQ=60)
                                                   CTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACTCCATTGGCAATATTCAGCAG                                            <  SRR4302540.7236292/94‑1 (MQ=60)
                                                   CTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAG                                            <  SRR4302540.6281995/94‑1 (MQ=60)
                                                   CTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAG                                            <  SRR4302540.9576711/94‑1 (MQ=60)
                                                   CTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAG                                            >  SRR4302540.2966729/1‑94 (MQ=60)
                                                   CTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAG                                            <  SRR4302540.3928776/94‑1 (MQ=60)
                                                   CTTTCCGCTATTTCTTTTATTCACCCTCCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAG                                            <  SRR4302540.4309746/94‑1 (MQ=60)
                                                   CTTTCCGCGATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAG                                            <  SRR4302540.8163086/94‑1 (MQ=60)
                                                    TTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGG                                           >  SRR4302540.667695/1‑94 (MQ=60)
                                                    TTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGG                                           <  SRR4302540.9001430/94‑1 (MQ=60)
                                                    TTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGG                                           >  SRR4302540.9272519/1‑94 (MQ=60)
                                                    TTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGG                                           >  SRR4302540.2062122/1‑94 (MQ=60)
                                                     TTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTGAGCAGGC                                          <  SRR4302540.1689029/94‑1 (MQ=60)
                                                     TTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGC                                          <  SRR4302540.2986735/94‑1 (MQ=60)
                                                     TTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGC                                          <  SRR4302540.2986734/94‑1 (MQ=60)
                                                      TCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCG                                         <  SRR4302540.10143467/94‑1 (MQ=60)
                                                        CGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGAT                                       >  SRR4302540.7320177/1‑94 (MQ=60)
                                                        CGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGAT                                       <  SRR4302540.2239327/94‑1 (MQ=60)
                                                        CGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGCCGAT                                       >  SRR4302540.4958443/1‑94 (MQ=60)
                                                         cgTATTTCTTTTATTCACCCTGCTCCCGCTGTC‑C‑ACCG‑TTCCGGCCCAGTCCGATATTATCGACTACACCAGTGGCAATATTCAGCAGGCGATT                                      <  SRR4302540.5833162/92‑1 (MQ=60)
                                                         GCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATT                                      <  SRR4302540.5833163/94‑1 (MQ=60)
                                                          CTATTTCTTTTTTT‑‑CCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAAT                                   <  SRR4302540.1051388/94‑1 (MQ=60)
                                                          CTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTA                                     <  SRR4302540.7156532/94‑1 (MQ=60)
                                                          CTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTA                                     >  SRR4302540.392152/1‑94 (MQ=60)
                                                          CTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATCA                                     >  SRR4302540.1550222/1‑94 (MQ=60)
                                                           TATTTCTTTTATTCCCCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAA                                    <  SRR4302540.7374079/94‑1 (MQ=60)
                                                           TATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCTGGCCCAGGCCGATATTATCGCCGACACCATTGGCAATATTCAGCAGGCGcttag                                    >  SRR4302540.5540500/1‑89 (MQ=60)
                                                           TATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAA                                    >  SRR4302540.203296/1‑94 (MQ=60)
                                                           TATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAA                                    <  SRR4302540.4278888/94‑1 (MQ=60)
                                                            ATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAAT                                   <  SRR4302540.1559019/94‑1 (MQ=60)
                                                            ATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAAT                                   >  SRR4302540.4539479/1‑94 (MQ=60)
                                                            ATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAAT                                   >  SRR4302540.4151638/1‑94 (MQ=60)
                                                            ATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAAT                                   <  SRR4302540.3734701/94‑1 (MQ=60)
                                                             TTTCTTTTATTCCCCCTTCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATG                                  <  SRR4302540.1613141/94‑1 (MQ=60)
                                                             TTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATG                                  >  SRR4302540.6553551/1‑94 (MQ=60)
                                                             TTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATG                                  <  SRR4302540.3948142/94‑1 (MQ=60)
                                                             TTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATG                                  <  SRR4302540.7372366/94‑1 (MQ=60)
                                                             TTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATG                                  <  SRR4302540.10020401/94‑1 (MQ=60)
                                                             TTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATA                                  <  SRR4302540.8820401/94‑1 (MQ=60)
                                                              TTCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGA                                 >  SRR4302540.4434286/1‑94 (MQ=60)
                                                               TCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGAC                                >  SRR4302540.5260627/1‑94 (MQ=60)
                                                               TCTTTTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGAC                                <  SRR4302540.5766556/94‑1 (MQ=60)
                                                               TCTTGTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGAC                                <  SRR4302540.5766555/94‑1 (MQ=60)
                                                                   TTATTCGCCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTT                            <  SRR4302540.3156227/94‑1 (MQ=60)
                                                                   TTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTT                            <  SRR4302540.4549549/94‑1 (MQ=60)
                                                                   TTATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTT                            >  SRR4302540.3579661/1‑94 (MQ=60)
                                                                    TATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTT                           >  SRR4302540.1943535/1‑94 (MQ=60)
                                                                    TATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTA                           >  SRR4302540.5136828/1‑94 (MQ=60)
                                                                    TATTAACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTA                           <  SRR4302540.6386869/94‑1 (MQ=60)
                                                                     ATTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTAT                          >  SRR4302540.3783117/1‑94 (MQ=60)
                                                                      TTCCCCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATA                         <  SRR4302540.518236/94‑1 (MQ=60)
                                                                      TTCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATA                         <  SRR4302540.8049962/94‑1 (MQ=60)
                                                                       TCCCCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAA                        <  SRR4302540.2949903/94‑1 (MQ=60)
                                                                       TCACCCTGCTCACGCTGTC‑CTC‑CT‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAA                        <  SRR4302540.1722961/94‑1 (MQ=60)
                                                                       TCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAA                        <  SRR4302540.4826460/94‑1 (MQ=60)
                                                                       TCACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAA                        <  SRR4302540.5288696/94‑1 (MQ=60)
                                                                        CACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAAC                       >  SRR4302540.5724467/1‑94 (MQ=60)
                                                                        CACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAAC                       <  SRR4302540.9601967/94‑1 (MQ=60)
                                                                         CCCCTGCTCAGGCTGTC‑C‑ACCG‑GTCCGGCCCAGTCTGATATTATCGACGACACCATCGGCACTATTCAGCAGGCGATTAATGACGCTTATAACC                      <  SRR4302540.4205367/94‑1 (MQ=60)
                                                                         ACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACC                      <  SRR4302540.4521379/94‑1 (MQ=60)
                                                                         ACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACC                      <  SRR4302540.4586051/94‑1 (MQ=60)
                                                                         ACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACC                      >  SRR4302540.7223962/1‑94 (MQ=60)
                                                                         ACCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACC                      >  SRR4302540.4446123/1‑94 (MQ=60)
                                                                          CCCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCC                     <  SRR4302540.9468794/94‑1 (MQ=60)
                                                                           CCTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCT                    <  SRR4302540.8305071/94‑1 (MQ=60)
                                                                            CTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATAatatcggcctggaagcgctagatcggaagagcg                   >  SRR4302540.7100583/1‑61 (MQ=60)
                                                                            CTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTG                   <  SRR4302540.7615330/94‑1 (MQ=60)
                                                                            CTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTG                   >  SRR4302540.4737529/1‑94 (MQ=60)
                                                                            CTGCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTA                   >  SRR4302540.5256175/1‑94 (MQ=60)
                                                                              GCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGAC                 >  SRR4302540.4242467/1‑94 (MQ=60)
                                                                              GCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGAC                 >  SRR4302540.6835026/1‑94 (MQ=60)
                                                                              GCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGAC                 <  SRR4302540.10135550/94‑1 (MQ=60)
                                                                              GCTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGAC                 <  SRR4302540.2959418/94‑1 (MQ=60)
                                                                               CTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACC                <  SRR4302540.3343383/94‑1 (MQ=60)
                                                                               CTCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACC                <  SRR4302540.1229873/94‑1 (MQ=60)
                                                                                TCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCG               <  SRR4302540.8498866/94‑1 (MQ=60)
                                                                                TCACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCG               >  SRR4302540.4459058/1‑94 (MQ=60)
                                                                                 CACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGT              <  SRR4302540.6245533/94‑1 (MQ=60)
                                                                                 CACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGG              >  SRR4302540.2803451/1‑94 (MQ=60)
                                                                                 CACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGC              >  SRR4302540.10017700/1‑94 (MQ=60)
                                                                                  ACGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTG             >  SRR4302540.759092/1‑94 (MQ=60)
                                                                                   CGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGG            <  SRR4302540.6197785/94‑1 (MQ=60)
                                                                                   CGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGG            <  SRR4302540.4473520/94‑1 (MQ=60)
                                                                                   CGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGG            <  SRR4302540.4467781/94‑1 (MQ=60)
                                                                                   CGCTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGG            <  SRR4302540.4082762/94‑1 (MQ=60)
                                                                                   CGCTGTC‑C‑ACCG‑TGCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGG            <  SRR4302540.4467782/94‑1 (MQ=60)
                                                                                    GCTGTC‑CTC‑CG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGG           <  SRR4302540.8109224/94‑1 (MQ=60)
                                                                                     CTGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGC          <  SRR4302540.258253/94‑1 (MQ=60)
                                                                                      TGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGCG         >  SRR4302540.6632652/1‑94 (MQ=60)
                                                                                      TGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGCG         <  SRR4302540.1513722/94‑1 (MQ=60)
                                                                                      TGTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGCG         >  SRR4302540.1656597/1‑94 (MQ=60)
                                                                                       GTC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATGGACGACACGATTGGCAATATTACGCATGAGATTAATGACGCTTATAACCCTGACCGTGGGCGG        >  SRR4302540.4382389/1‑94 (MQ=60)
                                                                                        TC‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGCGAG       <  SRR4302540.1270224/94‑1 (MQ=60)
                                                                                         C‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGCGAGA      >  SRR4302540.3288899/1‑94 (MQ=60)
                                                                                         C‑C‑ACCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGCGAGA      >  SRR4302540.9994757/1‑94 (MQ=60)
                                                                                           C‑C‑CG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGCGAGACT    <  SRR4302540.2345193/94‑1 (MQ=60)
                                                                                             acCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGCGAGACT    <  SRR4302540.9919779/92‑1 (MQ=60)
                                                                                             acCG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGCGAGACT    >  SRR4302540.9346894/3‑94 (MQ=60)
                                                                                               CG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGCGAGACTAT  <  SRR4302540.2283284/94‑1 (MQ=60)
                                                                                               CG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGCGAGACTAT  >  SRR4302540.10078510/1‑94 (MQ=60)
                                                                                               |                                                                                              
AGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTC‑C‑A‑CG‑TTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGCGAGACTAT  >  NC_000913/4311404‑4311589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑