Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,316,203 T→A Q47L (CAG→CTG)  phnK ← ATP‑binding component of phosphonate transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,316,2030TA99.6% 924.5 / NA 255Q47L (CAG→CTG) phnKATP‑binding component of phosphonate transport
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (145/109);  minor base C (0/1);  total (145/110)
Fisher's exact test for biased strand distribution p-value = 4.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 276 total aligned reads displayed.

GGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCTGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCC  >  NC_000913/4316114‑4316292
                                                                                          |                                                                                         
 gTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGTGCGGAGATCGACTTCAGCAGCGt                                                                                       >  2:5612346/1‑93 (MQ=255)
 gTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGt                                                                                       >  1:4729259/1‑93 (MQ=255)
 gTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCCACTTCAGCAGCGt                                                                                       >  1:2749017/1‑93 (MQ=255)
  tCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTg                                                                                      <  2:1618958/93‑1 (MQ=255)
  tCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTg                                                                                      <  1:5196509/93‑1 (MQ=255)
   cGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTgg                                                                                     >  2:9103786/1‑93 (MQ=255)
   cGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTgg                                                                                     <  1:3681155/93‑1 (MQ=255)
     gCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCAACTTCAGCAGCGTGGTc                                                                                   <  1:4414554/93‑1 (MQ=255)
       cTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTAGTCt                                                                                  >  2:9980161/1‑93 (MQ=255)
       cTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCtt                                                                                 >  2:2514613/1‑93 (MQ=255)
        tCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTc                                                                                >  1:8796848/1‑93 (MQ=255)
        tCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTc                                                                                <  1:5963742/93‑1 (MQ=255)
         cGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTcc                                                                               >  1:1998566/1‑93 (MQ=255)
          gCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTccc                                                                              >  1:9876123/1‑93 (MQ=255)
           cTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCg                                                                             >  2:8188113/1‑93 (MQ=255)
            tCATTGCATACAGCGAACGGTTCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCgg                                                                            >  2:7064104/1‑93 (MQ=255)
            tCATTGCATACAGCGAACGGTTCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCgg                                                                            >  2:1968466/1‑93 (MQ=255)
               ttGCATACAGCGAACGGTTCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGc                                                                         >  1:676098/1‑93 (MQ=255)
               ttGCATACAGCGAACGGTTCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGc                                                                         >  1:1669389/1‑93 (MQ=255)
                 tcATACAGCGAACGGTTCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCg                                                                       <  1:5692677/92‑1 (MQ=255)
                   aTACAGCGAACGGTTCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGa                                                                     >  2:9403640/1‑93 (MQ=255)
                   aTACAGCGAACGGTTCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGa                                                                     <  1:7391080/93‑1 (MQ=255)
                   aTACAGCGAACGGTTCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGa                                                                     <  1:3844987/93‑1 (MQ=255)
                   aTACAGCGAACGGTTCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGa                                                                     >  1:325299/1‑93 (MQ=255)
                    tACAGCGAACGGTTCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGAt                                                                    <  2:6808465/93‑1 (MQ=255)
                    tACAGCGAACGGTTCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGAt                                                                    >  1:1256068/1‑93 (MQ=255)
                     aCAGCGAACGGTTCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGAtt                                                                   >  1:8377997/1‑93 (MQ=255)
                         cGAACGGTTCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTcccc                                                               <  2:2956718/93‑1 (MQ=255)
                          gAACGGTTCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCg                                                              >  1:8733147/1‑93 (MQ=255)
                          gAACGGTTCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCg                                                              <  1:3108188/93‑1 (MQ=255)
                             cGGTTCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACa                                                           >  2:2417360/1‑93 (MQ=255)
                              ggTTCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACaa                                                          >  2:7455162/1‑93 (MQ=255)
                              ggTTCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACaa                                                          <  2:7879738/93‑1 (MQ=255)
                               gTTCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGTATTCCCCGACAAt                                                         >  1:9324104/1‑93 (MQ=255)
                               gTTCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACaag                                                         >  1:7726513/1‑92 (MQ=255)
                               gTTCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAAt                                                         >  2:9915744/1‑93 (MQ=255)
                               gTTCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAAt                                                         >  2:124113/1‑93 (MQ=255)
                                ttCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATg                                                        >  1:6371670/1‑93 (MQ=255)
                                   tcGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGccc                                                     >  1:226703/1‑93 (MQ=255)
                                     gTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAg                                                   >  2:665141/1‑93 (MQ=255)
                                     gTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAg                                                   <  1:5437776/93‑1 (MQ=255)
                                      tAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGc                                                  <  2:4437085/93‑1 (MQ=255)
                                       aGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCa                                                 <  2:3176541/93‑1 (MQ=255)
                                        gTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCAc                                                >  2:6137695/1‑93 (MQ=255)
                                        gTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCAc                                                >  1:1905943/1‑93 (MQ=255)
                                         tGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACt                                               <  2:6625700/93‑1 (MQ=255)
                                         tGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACt                                               >  2:415058/1‑93 (MQ=255)
                                           aaTTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTc                                             <  2:4251409/93‑1 (MQ=255)
                                           aaTTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTc                                             >  2:257933/1‑93 (MQ=255)
                                           aaTTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTc                                             >  1:2405478/1‑93 (MQ=255)
                                            aTTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTcc                                            >  1:5459695/1‑93 (MQ=255)
                                            aTTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTcc                                            <  2:1707268/93‑1 (MQ=255)
                                            aTTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTcc                                            <  2:2810550/93‑1 (MQ=255)
                                             ttt‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTccc                                           <  1:8225537/93‑1 (MQ=255)
                                              tt‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTcccc                                          >  1:5794947/1‑93 (MQ=255)
                                               t‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACACTGCCCAGCCCTTccccc                                         >  1:9050559/1‑93 (MQ=255)
                                               t‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACACTGCCCAGCACTTccccc                                         >  1:7251768/1‑93 (MQ=255)
                                                  ccccTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCgc                                       >  1:8586710/1‑92 (MQ=255)
                                                    ccTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGcc                                     >  2:4493456/1‑93 (MQ=255)
                                                     ctgctgCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCa                                    >  1:9629211/1‑93 (MQ=255)
                                                      tgctgcGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCAt                                   >  1:6836396/1‑93 (MQ=255)
                                                       gctgcGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATa                                  >  1:7696240/1‑93 (MQ=255)
                                                         tgcGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATaaa                                >  2:9528548/1‑93 (MQ=255)
                                                           cggcgTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATTCCCAGCACTTCCCCCGGCCATAAATc                              <  1:550046/93‑1 (MQ=255)
                                                            ggcgTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCa                             >  2:2672693/1‑93 (MQ=255)
                                                            ggcgTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCa                             >  2:1108327/1‑93 (MQ=255)
                                                            ggcgTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCa                             >  2:3790922/1‑93 (MQ=255)
                                                            ggcgTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCa                             >  2:8020842/1‑93 (MQ=255)
                                                              cgTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCaaa                           <  2:3885015/93‑1 (MQ=255)
                                                                tCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAag                         <  2:5107072/93‑1 (MQ=255)
                                                                 cAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAaga                        <  2:3692067/93‑1 (MQ=255)
                                                                   ggCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAgaga                      <  2:178683/93‑1 (MQ=255)
                                                                   ggCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAgaga                      <  2:7417650/93‑1 (MQ=255)
                                                                    gcgcgcGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGAc                     <  1:1087669/93‑1 (MQ=255)
                                                                    gcgcgcGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGAc                     <  1:4772648/93‑1 (MQ=255)
                                                                     cgcgcgGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACa                    <  2:5399263/93‑1 (MQ=255)
                                                                      gcgcgGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAt                   >  2:5246526/1‑93 (MQ=255)
                                                                        gcgGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCg                 >  1:183828/1‑93 (MQ=255)
                                                                        gcgGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCg                 >  1:3549129/1‑93 (MQ=255)
                                                                         cgGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGc                <  1:156589/93‑1 (MQ=255)
                                                                             gaTCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATTCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTaaa            >  2:577428/1‑93 (MQ=255)
                                                                             gaTCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTaaa            >  2:4357780/1‑93 (MQ=255)
                                                                              aTCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAg           <  1:4577318/93‑1 (MQ=255)
                                                                               tCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGc          <  1:1432640/93‑1 (MQ=255)
                                                                               tCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGc          >  2:4312461/1‑93 (MQ=255)
                                                                                 gACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCt        <  2:48471/93‑1 (MQ=255)
                                                                                 gACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCt        <  1:1214317/93‑1 (MQ=255)
                                                                                  aCTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCtt       >  2:2096687/1‑93 (MQ=255)
                                                                                  aCTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCtt       >  1:10016271/1‑93 (MQ=255)
                                                                                  aCTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCtt       >  1:9403210/1‑93 (MQ=255)
                                                                                  aCTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCtt       <  1:7931549/93‑1 (MQ=255)
                                                                                  aCTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCtt       >  2:9041939/1‑93 (MQ=255)
                                                                                   cTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAATGAGACATCGCTAAAGCCttt      <  2:9682717/93‑1 (MQ=255)
                                                                                    ttCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTg     <  1:6514845/93‑1 (MQ=255)
                                                                                     tCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGc    <  2:5867246/93‑1 (MQ=255)
                                                                                     tCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGc    <  2:852721/93‑1 (MQ=255)
                                                                                      cagcagCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGcc   >  1:9067532/1‑93 (MQ=255)
                                                                                      cAGCGGCGTGTTCTTCCCGGTCGCGGATACCCCGACAATGCCAAGCATTGCCACCGGCCATAAATCAAAAGAGACATCGAGAAAGCCTTTGcc   <  2:6276324/93‑1 (MQ=255)
                                                                                       agcagcGTGGTCTTCCCGGAGCCGGATCCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGccc  <  1:1365450/93‑1 (MQ=255)
                                                                                       agcagcGTGGTCTTCCCGGAGCCGGATCCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGccc  <  1:29955/93‑1 (MQ=255)
                                                                                          |                                                                                         
GGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTT‑CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCTGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCC  >  NC_000913/4316114‑4316292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCTGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCCGGC  >  NC_000913/4316110‑4316295
                                                                                             |                                                                                            
CGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCA                                                                                              >  SRR4302540.9880336/1‑94 (MQ=60)
CGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCA                                                                                              <  SRR4302540.8029316/94‑1 (MQ=60)
 GGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAG                                                                                             >  SRR4302540.5623510/1‑94 (MQ=60)
 GGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAG                                                                                             >  SRR4302540.4229483/1‑94 (MQ=60)
  GCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGC                                                                                            <  SRR4302540.1010990/94‑1 (MQ=60)
    GGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGT                                                                                          >  SRR4302540.4779764/1‑94 (MQ=60)
    GGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCCACTTCAGCAGCGT                                                                                          >  SRR4302540.2778718/1‑94 (MQ=60)
    GGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGACGTCAGGCGCGCGGAGATCGACTTCAGCAGCGT                                                                                          >  SRR4302540.9785943/1‑94 (MQ=60)
      TCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGG                                                                                        <  SRR4302540.7784082/94‑1 (MQ=60)
      TCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGG                                                                                        <  SRR4302540.5253098/94‑1 (MQ=60)
      TCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAACAGCGTGG                                                                                        <  SRR4302540.3692516/94‑1 (MQ=60)
       CGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGT                                                                                       >  SRR4302540.2874087/1‑94 (MQ=60)
       CGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGT                                                                                       <  SRR4302540.3721390/94‑1 (MQ=60)
        GGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTC                                                                                      <  SRR4302540.1018147/94‑1 (MQ=60)
         GCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCAACTTCAGCAGCGTGGTCT                                                                                     <  SRR4302540.4462396/94‑1 (MQ=60)
          CCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTT                                                                                    <  SRR4302540.1372574/94‑1 (MQ=60)
           CTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTC                                                                                   <  SRR4302540.4585811/94‑1 (MQ=60)
           CTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTC                                                                                   >  SRR4302540.8073622/1‑94 (MQ=60)
           CTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTC                                                                                   >  SRR4302540.8898568/1‑94 (MQ=60)
           CTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTC                                                                                   >  SRR4302540.9606641/1‑94 (MQ=60)
           CTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGAGCGACTTCAGCAGCGTGGTCTTC                                                                                   >  SRR4302540.1617362/1‑94 (MQ=60)
            TCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCC                                                                                  <  SRR4302540.6029724/94‑1 (MQ=60)
            TCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCC                                                                                  >  SRR4302540.2020214/1‑94 (MQ=60)
             CGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCC                                                                                 >  SRR4302540.9991465/1‑94 (MQ=60)
              GCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCG                                                                                >  SRR4302540.8226122/1‑94 (MQ=60)
              GCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCG                                                                                >  SRR4302540.2395082/1‑94 (MQ=60)
              GCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTCCCCG                                                                                <  SRR4302540.8795268/94‑1 (MQ=60)
               CTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGG                                                                               >  SRR4302540.3731811/1‑94 (MQ=60)
               CTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGG                                                                               >  SRR4302540.214489/1‑94 (MQ=60)
                TCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGA                                                                              >  SRR4302540.10033794/1‑94 (MQ=60)
                TCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGA                                                                              >  SRR4302540.1473190/1‑94 (MQ=60)
                TCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGA                                                                              >  SRR4302540.6719298/1‑94 (MQ=60)
                 CATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAG                                                                             <  SRR4302540.781344/94‑1 (MQ=60)
                 CATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAG                                                                             >  SRR4302540.8629510/1‑94 (MQ=60)
                  ATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGC                                                                            >  SRR4302540.1687554/1‑94 (MQ=60)
                  ATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGC                                                                            >  SRR4302540.683335/1‑94 (MQ=60)
                    TGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCG                                                                          >  SRR4302540.4767982/1‑94 (MQ=60)
                     TCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGG                                                                         <  SRR4302540.5755351/94‑1 (MQ=60)
                      CATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGA                                                                        >  SRR4302540.328560/1‑94 (MQ=60)
                       ATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGTAGCCGGAT                                                                       >  SRR4302540.1268642/1‑94 (MQ=60)
                       ATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGAT                                                                       <  SRR4302540.5555160/94‑1 (MQ=60)
                       ATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGAT                                                                       <  SRR4302540.3886798/94‑1 (MQ=60)
                       ATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGAT                                                                       <  SRR4302540.7473436/94‑1 (MQ=60)
                       ATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGAT                                                                       >  SRR4302540.1268940/1‑94 (MQ=60)
                       ATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGAT                                                                       <  SRR4302540.4817783/94‑1 (MQ=60)
                        TACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATT                                                                      >  SRR4302540.8475216/1‑94 (MQ=60)
                        TACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATT                                                                      <  SRR4302540.300076/94‑1 (MQ=60)
                        TACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATT                                                                      <  SRR4302540.9729307/94‑1 (MQ=60)
                         ACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTC                                                                     <  SRR4302540.4053754/94‑1 (MQ=60)
                         ACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTC                                                                     >  SRR4302540.5267730/1‑94 (MQ=60)
                           AGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCC                                                                   >  SRR4302540.3064416/1‑94 (MQ=60)
                           AGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCC                                                                   <  SRR4302540.1125971/94‑1 (MQ=60)
                            GCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCC                                                                  <  SRR4302540.1406465/94‑1 (MQ=60)
                             CGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCG                                                                 >  SRR4302540.8834561/1‑94 (MQ=60)
                              GAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGA                                                                <  SRR4302540.5323654/94‑1 (MQ=60)
                              GAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGA                                                                <  SRR4302540.3142204/94‑1 (MQ=60)
                               AACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGAC                                                               <  SRR4302540.8367587/94‑1 (MQ=60)
                                ACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACA                                                              >  SRR4302540.6790898/1‑94 (MQ=60)
                                 CGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGCTTCCCCGACAA                                                             >  SRR4302540.441109/1‑94 (MQ=60)
                                 CGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAA                                                             >  SRR4302540.3566096/1‑94 (MQ=60)
                                  GGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGTATTCCCCGACAAT                                                            >  SRR4302540.9435329/1‑94 (MQ=60)
                                  GGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAAG                                                            >  SRR4302540.7816703/1‑94 (MQ=60)
                                   GTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATG                                                           <  SRR4302540.8751095/94‑1 (MQ=60)
                                   GTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATG                                                           >  SRR4302540.6442617/1‑94 (MQ=60)
                                    TTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGCCAATGC                                                          >  SRR4302540.2614454/1‑94 (MQ=60)
                                    TTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGC                                                          >  SRR4302540.2306227/1‑94 (MQ=60)
                                    TTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGC                                                          >  SRR4302540.9960343/1‑94 (MQ=60)
                                    TTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGC                                                          <  SRR4302540.5711842/94‑1 (MQ=60)
                                      CTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCC                                                        >  SRR4302540.228891/1‑94 (MQ=60)
                                       TCGTAGTGATTTTCCCGCTGCTGCGGCGTCAGGCGTGCGGAGATCGACTTCAGCCGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCA                                                       <  SRR4302540.6414757/94‑1 (MQ=60)
                                        CGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAG                                                      >  SRR4302540.9013409/1‑94 (MQ=60)
                                         GTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGC                                                     >  SRR4302540.9488395/1‑94 (MQ=60)
                                         GTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGC                                                     <  SRR4302540.8888639/94‑1 (MQ=60)
                                         GTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGC                                                     >  SRR4302540.5851487/1‑94 (MQ=60)
                                         GTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGC                                                     <  SRR4302540.4663631/94‑1 (MQ=60)
                                         GTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGC                                                     >  SRR4302540.1584229/1‑94 (MQ=60)
                                         GTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGC                                                     <  SRR4302540.5497595/94‑1 (MQ=60)
                                          TAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGCTTCCCCGACAATG‑CCAGCAC                                                   >  SRR4302540.1793211/1‑94 (MQ=60)
                                          TAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCA                                                    >  SRR4302540.3171651/1‑94 (MQ=60)
                                          TAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCA                                                    >  SRR4302540.1543637/1‑94 (MQ=60)
                                           AGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCAC                                                   >  SRR4302540.1926775/1‑94 (MQ=60)
                                           AGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCAC                                                   <  SRR4302540.9390225/94‑1 (MQ=60)
                                           AGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCAC                                                   >  SRR4302540.1926776/1‑94 (MQ=60)
                                              GAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTC                                                >  SRR4302540.1940641/1‑94 (MQ=60)
                                              GAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTC                                                >  SRR4302540.2431418/1‑94 (MQ=60)
                                               AATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCCTTTCC                                               >  SRR4302540.5519786/1‑94 (MQ=60)
                                               AATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCC                                               <  SRR4302540.5797194/94‑1 (MQ=60)
                                               AATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCC                                               >  SRR4302540.5519785/1‑94 (MQ=60)
                                                ATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCC                                              <  SRR4302540.5506466/94‑1 (MQ=60)
                                                 TTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCC                                             >  SRR4302540.5858980/1‑94 (MQ=60)
                                                 TTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCC                                             <  SRR4302540.8321157/94‑1 (MQ=60)
                                                 TTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCC                                             <  SRR4302540.8321158/94‑1 (MQ=60)
                                                  TTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACACTGCCCAGCCCTTCCCCC                                            >  SRR4302540.9159204/1‑94 (MQ=60)
                                                  TTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACACTGCCCAGCACTTCCCCC                                            >  SRR4302540.7332689/1‑94 (MQ=60)
                                                  TTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCC                                            >  SRR4302540.9159485/1‑94 (MQ=60)
                                                   TCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCG                                           <  SRR4302540.5251046/94‑1 (MQ=60)
                                                    CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGC                                          >  SRR4302540.8686529/1‑94 (MQ=60)
                                                     CCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGC                                         >  SRR4302540.3148775/1‑94 (MQ=60)
                                                      CCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGAGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCCCTTCCTCCGGCC                                        >  SRR4302540.6690929/1‑94 (MQ=60)
                                                       CCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCA                                       >  SRR4302540.9742680/1‑94 (MQ=60)
                                                        CTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCAT                                      >  SRR4302540.6912116/1‑94 (MQ=60)
                                                        CTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCAT                                      <  SRR4302540.5575265/94‑1 (MQ=60)
                                                        CTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCAT                                      >  SRR4302540.5088288/1‑94 (MQ=60)
                                                         TGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATA                                     <  SRR4302540.8007919/94‑1 (MQ=60)
                                                         TGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATA                                     >  SRR4302540.7783957/1‑94 (MQ=60)
                                                          GCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAA                                    >  SRR4302540.3051258/1‑94 (MQ=60)
                                                           CTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAA                                   >  SRR4302540.949563/1‑94 (MQ=60)
                                                            TGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAAT                                  >  SRR4302540.8864646/1‑94 (MQ=60)
                                                             GCGGCGTCAGGCGCGCGGAGATCGAGTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATC                                 <  SRR4302540.9427897/94‑1 (MQ=60)
                                                             GCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATC                                 <  SRR4302540.7625452/94‑1 (MQ=60)
                                                              CGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATTCCCAGCACTTCCCCCGGCCATAAATCA                                <  SRR4302540.556038/94‑1 (MQ=60)
                                                                    CAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAG                          >  SRR4302540.6498484/1‑94 (MQ=60)
                                                                      ggccgGCGGAGATCGACTTGAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGAC                        <  SRR4302540.4796392/89‑1 (MQ=60)
                                                                      GGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGAC                        <  SRR4302540.4796390/94‑1 (MQ=60)
                                                                       GCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACA                       <  SRR4302540.1098822/94‑1 (MQ=60)
                                                                       GCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACA                       <  SRR4302540.4823635/94‑1 (MQ=60)
                                                                          CGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACGATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCC                    >  SRR4302540.3587903/1‑94 (MQ=60)
                                                                          CGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCG                    >  SRR4302540.185657/1‑94 (MQ=60)
                                                                          CGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCG                    <  SRR4302540.7416728/94‑1 (MQ=60)
                                                                          CGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCG                    <  SRR4302540.9102589/94‑1 (MQ=60)
                                                                          CGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCG                    >  SRR4302540.3587900/1‑94 (MQ=60)
                                                                           GCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGC                   <  SRR4302540.740929/94‑1 (MQ=60)
                                                                           GCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGACTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGC                   <  SRR4302540.7267028/94‑1 (MQ=60)
                                                                            CGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCT                  <  SRR4302540.158179/94‑1 (MQ=60)
                                                                            CGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCT                  <  SRR4302540.6885652/94‑1 (MQ=60)
                                                                             GGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTA                 >  SRR4302540.125315/1‑94 (MQ=60)
                                                                             GGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTA                 >  SRR4302540.2276511/1‑94 (MQ=60)
                                                                              GAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAA                <  SRR4302540.7687534/94‑1 (MQ=60)
                                                                               AGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAA               >  SRR4302540.6118743/1‑94 (MQ=60)
                                                                                GATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATCCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAC              >  SRR4302540.1367513/1‑94 (MQ=60)
                                                                                 ATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGC             <  SRR4302540.4626685/94‑1 (MQ=60)
                                                                                  TCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCC            <  SRR4302540.1448166/94‑1 (MQ=60)
                                                                                    GACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTT          <  SRR4302540.1874267/94‑1 (MQ=60)
                                                                                    GACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTT          >  SRR4302540.10132864/1‑94 (MQ=60)
                                                                                    GACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTT          >  SRR4302540.9515063/1‑94 (MQ=60)
                                                                                    GACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTT          >  SRR4302540.1168523/1‑94 (MQ=60)
                                                                                    GACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTT          <  SRR4302540.1226763/94‑1 (MQ=60)
                                                                                     ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTT         >  SRR4302540.3749676/1‑94 (MQ=60)
                                                                                     ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTT         <  SRR4302540.8023998/94‑1 (MQ=60)
                                                                                      CTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTG        <  SRR4302540.6021765/94‑1 (MQ=60)
                                                                                       TTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGC       <  SRR4302540.6587218/94‑1 (MQ=60)
                                                                                       TTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGC       <  SRR4302540.6586924/94‑1 (MQ=60)
                                                                                        TCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCC      >  SRR4302540.9176409/1‑94 (MQ=60)
                                                                                         CAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCC     <  SRR4302540.1827441/94‑1 (MQ=60)
                                                                                          AGCAGCGTGGTCTTCCCGGAGCCGGATCCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCCG    <  SRR4302540.1379887/94‑1 (MQ=60)
                                                                                          AGCAGCGTGGTCTTCCCGGAGCCGGATCCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCCG    <  SRR4302540.30272/94‑1 (MQ=60)
                                                                                            CAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCCGGC  <  SRR4302540.2137835/94‑1 (MQ=60)
                                                                                             |                                                                                            
CGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCTGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCCGGC  >  NC_000913/4316110‑4316295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑