Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,316,203 T→A Q47L (CAG→CTG)  phnK ← ATP‑binding component of phosphonate transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,316,2030TA100.0% 869.0 / NA 236Q47L (CAG→CTG) phnKATP‑binding component of phosphonate transport
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (114/122);  total (114/122)

Only 100 of 253 total aligned reads displayed.

CGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCTGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCC  >  NC_000913/4316113‑4316292
                                                                                          |                                                                                         
cggTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCagcagc                                                                                         <  1:1109147/93‑1 (MQ=255)
 ggTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCg                                                                                        <  1:297842/93‑1 (MQ=255)
  gTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCgg                                                                                       >  1:624571/1‑92 (MQ=255)
  gTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGt                                                                                       >  1:5999518/1‑93 (MQ=255)
  gTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGt                                                                                       <  2:1074353/93‑1 (MQ=255)
  gTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGt                                                                                       <  1:1838499/93‑1 (MQ=255)
  gTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGAACGACTTCAGCAGCgg                                                                                       >  2:296226/1‑92 (MQ=255)
   tCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTg                                                                                      <  1:4789543/93‑1 (MQ=255)
    cGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTgg                                                                                     <  1:6093629/93‑1 (MQ=255)
    cGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTgg                                                                                     >  2:5494951/1‑93 (MQ=255)
     ggCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGt                                                                                    <  1:3889114/93‑1 (MQ=255)
      gCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTc                                                                                   >  2:4768333/1‑93 (MQ=255)
      gCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTc                                                                                   >  1:3748205/1‑93 (MQ=255)
        cTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCtt                                                                                 <  2:2787091/93‑1 (MQ=255)
         tCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTc                                                                                >  2:4551751/1‑93 (MQ=255)
         tCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTc                                                                                <  1:3329298/93‑1 (MQ=255)
         tCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTc                                                                                <  1:4887792/93‑1 (MQ=255)
           gCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTccc                                                                              <  2:500183/93‑1 (MQ=255)
            cTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCg                                                                             <  1:1756514/93‑1 (MQ=255)
             tCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCgg                                                                            >  1:4458656/1‑93 (MQ=255)
               aTTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAg                                                                          >  2:2668049/1‑93 (MQ=255)
               aTTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAg                                                                          >  2:2143499/1‑93 (MQ=255)
                ttGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGc                                                                         >  2:2219540/1‑93 (MQ=255)
                ttGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGc                                                                         >  1:2746769/1‑93 (MQ=255)
                ttGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGc                                                                         <  1:2497401/93‑1 (MQ=255)
                ttGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGc                                                                         >  2:3990206/1‑93 (MQ=255)
                  gCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCg                                                                       >  2:3546853/1‑93 (MQ=255)
                    aTACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGa                                                                     >  2:4036522/1‑93 (MQ=255)
                    aTACAGCGAACGGTTCTCGTAGTGAATTTACCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGa                                                                     >  2:977278/1‑93 (MQ=255)
                          cGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTcccc                                                               <  1:3407260/93‑1 (MQ=255)
                          cGAACGGTTCTCGTAGTGAATGTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTcccc                                                               <  2:4233885/93‑1 (MQ=255)
                           gAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCg                                                              >  2:3714881/1‑93 (MQ=255)
                           gAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCg                                                              <  2:4716897/93‑1 (MQ=255)
                            aaCGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGa                                                             <  2:2388880/93‑1 (MQ=255)
                             aCGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCTGGAGCCGGATTCCCCGaa                                                            >  2:4191705/1‑92 (MQ=255)
                             aCGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGAc                                                            <  1:3508199/93‑1 (MQ=255)
                             aCGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGAc                                                            >  2:1921463/1‑93 (MQ=255)
                               ggTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACaa                                                          <  1:743694/93‑1 (MQ=255)
                               ggTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACaa                                                          <  2:1858369/93‑1 (MQ=255)
                                 ttCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATg                                                        >  1:5318435/1‑93 (MQ=255)
                                 ttCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGACTTCCCGGAGCCGGATTCCCCGACACTg                                                        >  1:1497208/1‑93 (MQ=255)
                                  tctcGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGc                                                       >  1:315423/1‑93 (MQ=255)
                                   ctcGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGcc                                                      <  2:5192681/93‑1 (MQ=255)
                                    tcGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGccc                                                     >  1:469900/1‑93 (MQ=255)
                                     cGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCa                                                    <  2:1031504/93‑1 (MQ=255)
                                     cGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCa                                                    <  2:3621311/93‑1 (MQ=255)
                                      gTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAg                                                   >  2:5349698/1‑93 (MQ=255)
                                      gTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAg                                                   <  1:2465283/93‑1 (MQ=255)
                                        aGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCa                                                 >  2:6134203/1‑93 (MQ=255)
                                        aGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCa                                                 <  1:809216/93‑1 (MQ=255)
                                            aaTTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTc                                             <  1:5050203/93‑1 (MQ=255)
                                            aaTTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTc                                             <  1:4014925/93‑1 (MQ=255)
                                            aaTTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTc                                             >  2:759437/1‑93 (MQ=255)
                                              tttCCCCCTGCTGCGGCGTCAGGCGCGGGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTccc                                           <  2:3291567/93‑1 (MQ=255)
                                               ttCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTcccc                                          <  2:5505006/93‑1 (MQ=255)
                                                 cccccTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCg                                        >  1:5034626/1‑93 (MQ=255)
                                                 cccccTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCg                                        <  1:1580291/93‑1 (MQ=255)
                                                   cccTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTGCCCCGGc                                      >  1:1628706/1‑93 (MQ=255)
                                                   cccTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGc                                      >  2:5953100/1‑93 (MQ=255)
                                                    ccTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGcc                                     >  2:5115247/1‑93 (MQ=255)
                                                    ccTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGcc                                     >  1:4644027/1‑93 (MQ=255)
                                                     ctgctgCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCa                                    >  1:5260857/1‑93 (MQ=255)
                                                     ctgctgCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCa                                    >  1:2296304/1‑93 (MQ=255)
                                                     ctgctgCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCa                                    <  2:5588297/93‑1 (MQ=255)
                                                      tgctgcGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCAt                                   >  1:6232285/1‑93 (MQ=255)
                                                       gctgcGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGTTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATa                                  <  2:1536965/93‑1 (MQ=255)
                                                       gctgcGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATa                                  >  1:5480668/1‑93 (MQ=255)
                                                        ctgcGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATaa                                 <  2:5820476/93‑1 (MQ=255)
                                                        ctgcGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATaa                                 >  1:6120615/1‑93 (MQ=255)
                                                          gcggcgTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAAt                               >  1:1965974/1‑93 (MQ=255)
                                                           cggcgTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATc                              <  2:655826/93‑1 (MQ=255)
                                                             gcgTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCaa                            >  1:4556933/1‑93 (MQ=255)
                                                             gcgTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCaa                            <  1:5832045/93‑1 (MQ=255)
                                                             gcgTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCaa                            >  1:2166950/1‑93 (MQ=255)
                                                              cgTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCaaa                           <  2:4911954/93‑1 (MQ=255)
                                                               gTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCaaaa                          >  2:4655024/1‑93 (MQ=255)
                                                                tCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAag                         <  1:5332427/93‑1 (MQ=255)
                                                                 cAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAaga                        >  2:3156153/1‑93 (MQ=255)
                                                                 cAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAaga                        <  2:2549542/93‑1 (MQ=255)
                                                                   ggCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAgaga                      >  1:1420144/1‑93 (MQ=255)
                                                                   ggCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAgaga                      <  1:5599117/93‑1 (MQ=255)
                                                                    gcgcgcGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGAc                     <  2:4645889/93‑1 (MQ=255)
                                                                     cgcgcgGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACa                    <  2:5325469/93‑1 (MQ=255)
                                                                      gcgcgGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAt                   <  2:5681040/93‑1 (MQ=255)
                                                                      gcgcgGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAt                   <  2:5850378/93‑1 (MQ=255)
                                                                       cgcgGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATc                  <  1:1188201/93‑1 (MQ=255)
                                                                        gcgGAGATCGACTTCAGCAGCGTGGTCTTCCCGGGGCCGGATTCCCCGACAATGGCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCg                 >  2:3869476/1‑93 (MQ=255)
                                                                          ggAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCt               >  2:3419822/1‑93 (MQ=255)
                                                                           gagaTCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTa              >  2:3061183/1‑93 (MQ=255)
                                                                             gaTCTACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTGCCCCCGGCCATAAATCAAAAGAGACATCGCTaaa            >  2:3483068/1‑93 (MQ=255)
                                                                             gaTCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTaaa            >  1:2058768/1‑93 (MQ=255)
                                                                               tCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGc          <  2:5034818/93‑1 (MQ=255)
                                                                               tCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGc          <  1:4162660/93‑1 (MQ=255)
                                                                                cGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGcc         >  2:3818155/1‑93 (MQ=255)
                                                                                 gACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCt        <  2:3720963/93‑1 (MQ=255)
                                                                                    ttCAGCAGCGTGGTCTTCGCGGAGCCTGATACCCCGACAATGCCCAGCTCTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTg     <  2:1941050/93‑1 (MQ=255)
                                                                                    ttCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTg     >  2:4463839/1‑93 (MQ=255)
                                                                                     tCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGc    <  2:1312792/93‑1 (MQ=255)
                                                                                      cagcagCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGcc   <  1:3908007/93‑1 (MQ=255)
                                                                                       agcagcGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGccc  <  2:2300998/93‑1 (MQ=255)
                                                                                          |                                                                                         
CGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCTGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCC  >  NC_000913/4316113‑4316292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCTGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCCGGC  >  NC_000913/4316110‑4316295
                                                                                             |                                                                                            
CGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCA                                                                                              <  SRR4302533.1067366/94‑1 (MQ=60)
CGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCA                                                                                              <  SRR4302533.2466178/94‑1 (MQ=60)
 GGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAG                                                                                             <  SRR4302533.840172/94‑1 (MQ=60)
   CGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCG                                                                                           <  SRR4302533.1115731/94‑1 (MQ=60)
    GGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGT                                                                                          >  SRR4302533.628237/1‑94 (MQ=60)
    GGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGT                                                                                          >  SRR4302533.6035856/1‑94 (MQ=60)
    GGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGT                                                                                          <  SRR4302533.299666/94‑1 (MQ=60)
    GGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGT                                                                                          >  SRR4302533.214088/1‑94 (MQ=60)
     GTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTG                                                                                         <  SRR4302533.1849710/94‑1 (MQ=60)
     GTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTG                                                                                         <  SRR4302533.3991869/94‑1 (MQ=60)
      TCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGG                                                                                        <  SRR4302533.4818556/94‑1 (MQ=60)
      GCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGG                                                                                        <  SRR4302533.4811405/94‑1 (MQ=60)
       CGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGT                                                                                       <  SRR4302533.6130449/94‑1 (MQ=60)
        GGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTC                                                                                      <  SRR4302533.3912843/94‑1 (MQ=60)
        GGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTC                                                                                      >  SRR4302533.3771183/1‑94 (MQ=60)
        GGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTC                                                                                      >  SRR4302533.2495422/1‑94 (MQ=60)
          CCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTT                                                                                    <  SRR4302533.3932291/94‑1 (MQ=60)
          CCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTT                                                                                    <  SRR4302533.4535031/94‑1 (MQ=60)
           CTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTC                                                                                   >  SRR4302533.5509002/1‑94 (MQ=60)
            TCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCC                                                                                  <  SRR4302533.3349876/94‑1 (MQ=60)
            TCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCC                                                                                  <  SRR4302533.4917407/94‑1 (MQ=60)
            TCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCC                                                                                  >  SRR4302533.1579873/1‑94 (MQ=60)
             CGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCC                                                                                 >  SRR4302533.4664623/1‑94 (MQ=60)
             CGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGATGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCTC                                                                                 >  SRR4302533.3429235/1‑94 (MQ=60)
              GCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCG                                                                                <  SRR4302533.1850521/94‑1 (MQ=60)
               CTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGG                                                                               <  SRR4302533.1767216/94‑1 (MQ=60)
               CTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGG                                                                               >  SRR4302533.4485623/1‑94 (MQ=60)
                  ATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGC                                                                            >  SRR4302533.2763610/1‑94 (MQ=60)
                   TTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCC                                                                           <  SRR4302533.2512807/94‑1 (MQ=60)
                     GCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGG                                                                         <  SRR4302533.170940/94‑1 (MQ=60)
                      CATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGA                                                                        <  SRR4302533.177154/94‑1 (MQ=60)
                      CATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGA                                                                        >  SRR4302533.4818360/1‑94 (MQ=60)
                       ATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGAT                                                                       >  SRR4302533.4958886/1‑94 (MQ=60)
                        TACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATT                                                                      <  SRR4302533.5419244/94‑1 (MQ=60)
                        TACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATT                                                                      >  SRR4302533.3462878/1‑94 (MQ=60)
                        TACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATT                                                                      <  SRR4302533.3915920/94‑1 (MQ=60)
                        TACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATT                                                                      <  SRR4302533.2097415/94‑1 (MQ=60)
                            GCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCC                                                                  <  SRR4302533.5303771/94‑1 (MQ=60)
                            GCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCC                                                                  >  SRR4302533.1429096/1‑94 (MQ=60)
                             CGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCG                                                                 <  SRR4302533.3428305/94‑1 (MQ=60)
                             CGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCG                                                                 >  SRR4302533.958191/1‑94 (MQ=60)
                              GAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGA                                                                >  SRR4302533.5356622/1‑94 (MQ=60)
                              GAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGA                                                                >  SRR4302533.278266/1‑94 (MQ=60)
                                ACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACA                                                              <  SRR4302533.4618014/94‑1 (MQ=60)
                                ACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACA                                                              <  SRR4302533.3529831/94‑1 (MQ=60)
                                  GGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAAT                                                            <  SRR4302533.748070/94‑1 (MQ=60)
                                   GTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATG                                                           <  SRR4302533.1621142/94‑1 (MQ=60)
                                   GTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATG                                                           >  SRR4302533.5350308/1‑94 (MQ=60)
                                   GTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGACTTCCCGGAGCCGGATTCCCCGACACTG                                                           >  SRR4302533.1506224/1‑94 (MQ=60)
                                    TTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGC                                                          >  SRR4302533.317349/1‑94 (MQ=60)
                                    TTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGC                                                          >  SRR4302533.90599/1‑94 (MQ=60)
                                     TCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCC                                                         >  SRR4302533.5673799/1‑94 (MQ=60)
                                     TCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCC                                                         <  SRR4302533.365508/94‑1 (MQ=60)
                                      CTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCC                                                        >  SRR4302533.472708/1‑94 (MQ=60)
                                       TCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCA                                                       <  SRR4302533.1568179/94‑1 (MQ=60)
                                        CGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAG                                                      <  SRR4302533.6017551/94‑1 (MQ=60)
                                         GTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGC                                                     <  SRR4302533.2480490/94‑1 (MQ=60)
                                          TAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCA                                                    <  SRR4302533.5149785/94‑1 (MQ=60)
                                          TAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCA                                                    <  SRR4302533.3028062/94‑1 (MQ=60)
                                           AGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCAC                                                   <  SRR4302533.813966/94‑1 (MQ=60)
                                               AATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCC                                               <  SRR4302533.5080747/94‑1 (MQ=60)
                                               AATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCC                                               <  SRR4302533.4039374/94‑1 (MQ=60)
                                                 TTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCC                                             >  SRR4302533.3281311/1‑94 (MQ=60)
                                                   TCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTTTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCG                                           <  SRR4302533.4461931/94‑1 (MQ=60)
                                                   TCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTGCCCGACAATGCCCAGCACTTCCCCCG                                           >  SRR4302533.2409138/1‑94 (MQ=60)
                                                   TCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCG                                           >  SRR4302533.5065121/1‑94 (MQ=60)
                                                   TCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCG                                           <  SRR4302533.3365835/94‑1 (MQ=60)
                                                    CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGG                                          <  SRR4302533.1589809/94‑1 (MQ=60)
                                                    CCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGG                                          >  SRR4302533.4452473/1‑94 (MQ=60)
                                                     CCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTGCCCCGGC                                         >  SRR4302533.1638571/1‑94 (MQ=60)
                                                      CCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCC                                        >  SRR4302533.4672161/1‑94 (MQ=60)
                                                       CCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCA                                       >  SRR4302533.4852945/1‑94 (MQ=60)
                                                       CCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCA                                       >  SRR4302533.5292441/1‑94 (MQ=60)
                                                       CCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCA                                       >  SRR4302533.2310524/1‑94 (MQ=60)
                                                       CCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCA                                       >  SRR4302533.3807713/1‑94 (MQ=60)
                                                        CTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCAT                                      >  SRR4302533.6269608/1‑94 (MQ=60)
                                                         TGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATA                                     >  SRR4302533.5513711/1‑94 (MQ=60)
                                                          GCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAA                                    <  SRR4302533.1926909/94‑1 (MQ=60)
                                                          GCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAA                                    >  SRR4302533.6157523/1‑94 (MQ=60)
                                                          GCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAA                                    <  SRR4302533.5306667/94‑1 (MQ=60)
                                                           CTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAA                                   <  SRR4302533.1344651/94‑1 (MQ=60)
                                                            TGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAAT                                  <  SRR4302533.2559585/94‑1 (MQ=60)
                                                            TGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAAT                                  >  SRR4302533.1977988/1‑94 (MQ=60)
                                                             GCGGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATC                                 <  SRR4302533.3226730/94‑1 (MQ=60)
                                                               GGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAA                               >  SRR4302533.4584569/1‑94 (MQ=60)
                                                               GGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAA                               >  SRR4302533.6304598/1‑94 (MQ=60)
                                                               GGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAA                               >  SRR4302533.791718/1‑94 (MQ=60)
                                                               GGCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAA                               >  SRR4302533.2180277/1‑94 (MQ=60)
                                                                GCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAA                              <  SRR4302533.6132141/94‑1 (MQ=60)
                                                                GCGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAA                              <  SRR4302533.5867293/94‑1 (MQ=60)
                                                                 CGTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAA                             >  SRR4302533.6190287/1‑94 (MQ=60)
                                                                  GTCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAG                            >  SRR4302533.5048735/1‑94 (MQ=60)
                                                                   TCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGA                           <  SRR4302533.5364384/94‑1 (MQ=60)
                                                                   TCAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGA                           <  SRR4302533.633231/94‑1 (MQ=60)
                                                                    CAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAG                          <  SRR4302533.4684871/94‑1 (MQ=60)
                                                                    CAGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAG                          <  SRR4302533.5079457/94‑1 (MQ=60)
                                                                     AGGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGA                         >  SRR4302533.1428735/1‑94 (MQ=60)
                                                                      GGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGAC                        <  SRR4302533.5632947/94‑1 (MQ=60)
                                                                      GGCGCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGAC                        <  SRR4302533.612456/94‑1 (MQ=60)
                                                                         GCGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATC                     <  SRR4302533.6210408/94‑1 (MQ=60)
                                                                          CGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCG                    >  SRR4302533.3810134/1‑94 (MQ=60)
                                                                          CGCGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCG                    <  SRR4302533.1195335/94‑1 (MQ=60)
                                                                            CGGAGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCT                  >  SRR4302533.5753141/1‑94 (MQ=60)
                                                                               AGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAA               >  SRR4302533.1681505/1‑94 (MQ=60)
                                                                               AGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAA               >  SRR4302533.1990279/1‑94 (MQ=60)
                                                                               AGATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAA               >  SRR4302533.2071461/1‑94 (MQ=60)
                                                                                GATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAG              <  SRR4302533.2263487/94‑1 (MQ=60)
                                                                                GATCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACACCGCTAAAG              <  SRR4302533.4054328/94‑1 (MQ=60)
                                                                                  TCGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCC            <  SRR4302533.4187882/94‑1 (MQ=60)
                                                                                  TCGACTGCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCC            <  SRR4302533.1351780/94‑1 (MQ=60)
                                                                                   CGACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCT           >  SRR4302533.5258609/1‑94 (MQ=60)
                                                                                    GACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTT          >  SRR4302533.6107200/1‑94 (MQ=60)
                                                                                    GACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTT          <  SRR4302533.2330721/94‑1 (MQ=60)
                                                                                     ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTT         <  SRR4302533.1177560/94‑1 (MQ=60)
                                                                                     ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTT         <  SRR4302533.5612727/94‑1 (MQ=60)
                                                                                      CTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTG        >  SRR4302533.2666582/1‑94 (MQ=60)
                                                                                         CAGCATCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCC     <  SRR4302533.811882/94‑1 (MQ=60)
                                                                                         CAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCC     <  SRR4302533.3017709/94‑1 (MQ=60)
                                                                                         CAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCC     <  SRR4302533.3931876/94‑1 (MQ=60)
                                                                                            CAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCCGGC  <  SRR4302533.653376/94‑1 (MQ=60)
                                                                                             |                                                                                            
CGGCGGTCGGCCTCGCTCATTGCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGACTTCAGCTGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACATCGCTAAAGCCTTTGCCCGGC  >  NC_000913/4316110‑4316295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑