Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,422,211 T→C A53A (GCA→GCG)  yjfY ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,422,2110TC99.6% 1683.2 / ‑4.1 523A53A (GCA→GCG) yjfYhypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base C (254/267);  total (256/267)
Fisher's exact test for biased strand distribution p-value = 2.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01

Only 100 of 538 total aligned reads displayed.

CATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAAT  >  NC_000913/4422121‑4422300
                                                                                          |                                                                                         
cATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACgcgc                                                                                         <  2:9028896/93‑1 (MQ=255)
 atatTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCt                                                                                        <  1:4254023/93‑1 (MQ=255)
 atatTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCt                                                                                        >  1:940322/1‑93 (MQ=255)
  tatTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCtt                                                                                       <  2:5762044/93‑1 (MQ=255)
  tatTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCtt                                                                                       <  1:4608890/93‑1 (MQ=255)
  tatTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCtt                                                                                       >  1:3108609/1‑93 (MQ=255)
    ttGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCg                                                                                     >  2:7772442/1‑93 (MQ=255)
     tGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGc                                                                                    <  1:9806321/93‑1 (MQ=255)
     tGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGc                                                                                    <  2:7280326/93‑1 (MQ=255)
        cGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCttt                                                                                 <  1:6576781/93‑1 (MQ=255)
         gTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTc                                                                                >  1:1765306/1‑93 (MQ=255)
         gTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTc                                                                                <  1:5727898/93‑1 (MQ=255)
          ttACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCa                                                                               >  2:4991243/1‑93 (MQ=255)
           tccTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAg                                                                              <  2:706124/91‑1 (MQ=255)
           tACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAg                                                                              <  1:5600495/93‑1 (MQ=255)
            aCTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGt                                                                             >  2:6458371/1‑93 (MQ=255)
            aCTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGt                                                                             <  2:5176437/93‑1 (MQ=255)
                   ggTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGaaa                                                                      <  2:3373268/93‑1 (MQ=255)
                    gTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAAt                                                                     >  1:1048150/1‑93 (MQ=255)
                    gTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAAt                                                                     >  2:6073030/1‑93 (MQ=255)
                     ttCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAAtt                                                                    >  1:8509683/1‑93 (MQ=255)
                      tCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTa                                                                   <  2:3038374/93‑1 (MQ=255)
                         cGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTAtga                                                                <  1:3291260/93‑1 (MQ=255)
                          gCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAAGTAtgat                                                               >  1:4890671/1‑93 (MQ=255)
                           cATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTAtgatt                                                              >  2:1749541/1‑93 (MQ=255)
                            aTCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTAtgattg                                                             >  1:3011912/1‑93 (MQ=255)
                             tCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTAtgattga                                                            >  2:7534092/1‑93 (MQ=255)
                             tCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTAtgactga                                                            >  2:1312694/1‑93 (MQ=255)
                               aGAATTACGTGGTAGTACGTTGCGCCTTGCTCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGata                                                          <  1:2598897/93‑1 (MQ=255)
                               aGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGata                                                          <  2:9703336/93‑1 (MQ=255)
                               aGAATTACGTGGTAGTACGTTGCGCCTTGCGCAACTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGata                                                          <  1:8937069/93‑1 (MQ=255)
                                gAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGatat                                                         >  1:3602627/1‑93 (MQ=255)
                                  aTTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATaa                                                       <  2:8794494/93‑1 (MQ=255)
                                  aTTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATaa                                                       >  2:3253866/1‑93 (MQ=255)
                                  aTTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATaa                                                       <  2:4401035/93‑1 (MQ=255)
                                   ttACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATaaa                                                      <  1:3817951/93‑1 (MQ=255)
                                    tACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAAt                                                     >  2:956855/1‑93 (MQ=255)
                                    tACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAAt                                                     <  1:6145401/93‑1 (MQ=255)
                                     aCGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATc                                                    <  2:3537892/93‑1 (MQ=255)
                                      cGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCa                                                   >  2:4457947/1‑93 (MQ=255)
                                      cGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCa                                                   <  1:6001135/93‑1 (MQ=255)
                                      cGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCa                                                   <  1:1859161/93‑1 (MQ=255)
                                      cGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCa                                                   <  2:9357194/93‑1 (MQ=255)
                                       gTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCAc                                                  >  2:2419504/1‑93 (MQ=255)
                                        tGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACg                                                 >  1:2821423/1‑93 (MQ=255)
                                        tGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACg                                                 >  1:9602206/1‑93 (MQ=255)
                                        tGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACg                                                 >  2:4787487/1‑93 (MQ=255)
                                          gtagtaCGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGcc                                               >  2:4283518/1‑93 (MQ=255)
                                            agtaCGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTa                                             <  1:6919566/93‑1 (MQ=255)
                                            agtaCGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTa                                             <  2:4603452/93‑1 (MQ=255)
                                             gtaCGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTaa                                            <  2:9246495/93‑1 (MQ=255)
                                               aCGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGc                                          >  2:3841472/1‑93 (MQ=255)
                                                cGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCt                                         >  1:1511245/1‑93 (MQ=255)
                                                 gTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCtt                                        <  1:1181454/93‑1 (MQ=255)
                                                  ttGCGCGTTGCGCATCTGTTTCTTCATTGAATGCCTGACGCGCTTCGCGTTCAGTGGCGAAATTATGATTGAGATAAATCACGCCTAAGCttt                                       <  1:6744872/93‑1 (MQ=255)
                                                  ttGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCttt                                       <  1:4295594/93‑1 (MQ=255)
                                                     cgcCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGca                                    <  1:6359948/93‑1 (MQ=255)
                                                      gcCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGcac                                   >  2:7587507/1‑93 (MQ=255)
                                                        cTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACAt                                 >  2:5410529/1‑93 (MQ=255)
                                                        cTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACAt                                 >  2:5554435/1‑93 (MQ=255)
                                                         ttGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATc                                <  1:5240110/93‑1 (MQ=255)
                                                         ttGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATc                                <  2:2657210/93‑1 (MQ=255)
                                                         ttGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATc                                >  2:6674548/1‑93 (MQ=255)
                                                             gcATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTcc                            >  2:9836251/1‑93 (MQ=255)
                                                             gcATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTcc                            >  1:4379290/1‑93 (MQ=255)
                                                              cATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCa                           <  2:833256/93‑1 (MQ=255)
                                                              cATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCa                           >  2:5107875/1‑93 (MQ=255)
                                                               aTCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCat                          <  1:7802358/93‑1 (MQ=255)
                                                                tCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCata                         >  1:2490633/1‑93 (MQ=255)
                                                                tCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCata                         >  2:7374341/1‑93 (MQ=255)
                                                                tCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTACatt                         >  1:2283999/1‑92 (MQ=255)
                                                                tCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGACTGATATAAATCACGCCTAAGCTTTGCACATCGTCCata                         >  1:7875311/1‑93 (MQ=255)
                                                                  tGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATAtt                       >  1:8814871/1‑93 (MQ=255)
                                                                  tGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATAtt                       >  1:8139839/1‑93 (MQ=255)
                                                                  tGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATAtt                       >  2:6847565/1‑93 (MQ=255)
                                                                   gTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATAttt                      >  1:3978680/1‑93 (MQ=255)
                                                                    tttCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTc                     >  2:2231454/1‑93 (MQ=255)
                                                                    tttCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTc                     <  1:10010274/93‑1 (MQ=255)
                                                                    tttCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTc                     <  1:5431896/93‑1 (MQ=255)
                                                                    tttCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTc                     >  1:6658909/1‑93 (MQ=255)
                                                                     ttcttcATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCt                    >  2:1029152/1‑93 (MQ=255)
                                                                      tcttcATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTg                   >  2:8018453/1‑93 (MQ=255)
                                                                        ttcATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGc                 >  1:9523220/1‑93 (MQ=255)
                                                                         tcATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGcc                <  2:4180951/93‑1 (MQ=255)
                                                                         tcATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGcc                <  1:212336/93‑1 (MQ=255)
                                                                          cATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCt               >  1:8281619/1‑93 (MQ=255)
                                                                          cATTTAAGGCCTGACGCGCTTCGCTTTTAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCTCATCGTCCATATTTCTGGCCt               <  2:7682612/93‑1 (MQ=255)
                                                                           aTTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCtg              <  2:6254023/93‑1 (MQ=255)
                                                                           aTTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCtg              >  1:7449616/1‑93 (MQ=255)
                                                                            tttAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCtgg             <  1:9037987/93‑1 (MQ=255)
                                                                            tttAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCtgg             >  1:7152188/1‑93 (MQ=255)
                                                                             ttAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCtggt            >  2:7923810/1‑93 (MQ=255)
                                                                             ttAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCtggt            >  2:1481966/1‑93 (MQ=255)
                                                                             ttAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCtggt            <  2:2022907/93‑1 (MQ=255)
                                                                              tAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCtggtg           <  2:4921962/93‑1 (MQ=255)
                                                                               aaTGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCtggtgg          >  2:7164616/1‑93 (MQ=255)
                                                                                aTGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTggtggt         >  1:2566525/1‑93 (MQ=255)
                                                                                  gCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGtta       >  2:4747635/1‑93 (MQ=255)
                                                                                    cTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGttatt     <  1:4089774/93‑1 (MQ=255)
                                                                                      gACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTaa   >  1:531682/1‑93 (MQ=255)
                                                                                          |                                                                                         
CATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAAT  >  NC_000913/4422121‑4422300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 290 total aligned reads displayed.
GTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCA  >  NC_000913/4422118‑4422304
                                                                                             |                                                                                             
GTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGC                                                                                               >  SRR4302540.3034205/1‑94 (MQ=60)
GTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGC                                                                                               >  SRR4302540.3076899/1‑94 (MQ=60)
GTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGC                                                                                               >  SRR4302540.8114616/1‑94 (MQ=60)
 TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCG                                                                                              >  SRR4302540.7119284/1‑94 (MQ=60)
 TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGAGCATCTGTTTCTTCATTTAATGCCTGACGCG                                                                                              >  SRR4302540.5499919/1‑94 (MQ=60)
  TCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGC                                                                                             <  SRR4302540.207951/94‑1 (MQ=60)
  GCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGC                                                                                             <  SRR4302540.2174403/94‑1 (MQ=60)
  GCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGC                                                                                             >  SRR4302540.4304200/1‑94 (MQ=60)
  GCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGC                                                                                             <  SRR4302540.8383622/94‑1 (MQ=60)
    ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTT                                                                                           >  SRR4302540.3142629/1‑94 (MQ=60)
    ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTT                                                                                           <  SRR4302540.4866847/94‑1 (MQ=60)
    ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTT                                                                                           >  SRR4302540.9529348/1‑94 (MQ=60)
    ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTT                                                                                           <  SRR4302540.4300308/94‑1 (MQ=60)
     TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTC                                                                                          <  SRR4302540.4658377/94‑1 (MQ=60)
     TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTC                                                                                          >  SRR4302540.6418939/1‑94 (MQ=60)
      ATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCG                                                                                         <  SRR4302540.4669950/94‑1 (MQ=60)
        TGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCT                                                                                       <  SRR4302540.9921106/94‑1 (MQ=60)
         GCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTT                                                                                      <  SRR4302540.954862/94‑1 (MQ=60)
          CCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTT                                                                                     <  SRR4302540.8616231/94‑1 (MQ=60)
           CGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTC                                                                                    >  SRR4302540.3946512/1‑94 (MQ=60)
           CGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTC                                                                                    >  SRR4302540.8568088/1‑94 (MQ=60)
           CGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTC                                                                                    >  SRR4302540.9680767/1‑94 (MQ=60)
           CGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTC                                                                                    <  SRR4302540.3378861/94‑1 (MQ=60)
            GTTTCTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCA                                                                                   <  SRR4302540.601822/94‑1 (MQ=60)
            GTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCA                                                                                   <  SRR4302540.1694563/94‑1 (MQ=60)
            GTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCA                                                                                   >  SRR4302540.9137815/1‑94 (MQ=60)
            GTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCA                                                                                   >  SRR4302540.9137814/1‑94 (MQ=60)
            GTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCA                                                                                   <  SRR4302540.3670072/94‑1 (MQ=60)
            GTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCA                                                                                   <  SRR4302540.5790975/94‑1 (MQ=60)
             ttcccCCCCGTTTCCCGCATCAGATTTACGTGGTAGTACGTTCCGCCTTGCGCATCTGTTTCTTCATTAAATGCCTGACGCGCTTCGCTTTCAG                                                                                  <  SRR4302540.7386616/89‑1 (MQ=60)
              TACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGT                                                                                 <  SRR4302540.5107058/94‑1 (MQ=60)
              TACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGT                                                                                 >  SRR4302540.4324688/1‑94 (MQ=60)
              TACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGT                                                                                 <  SRR4302540.5662115/94‑1 (MQ=60)
              TACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGT                                                                                 <  SRR4302540.2957578/94‑1 (MQ=60)
              TACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGT                                                                                 <  SRR4302540.2419774/94‑1 (MQ=60)
               cccCCCCGGTTCCCGCACCAAAATTACGTGGTAGTACGTTGCCCCTTGCGCATTTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTG                                                                                <  SRR4302540.217145/91‑1 (MQ=60)
               ACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTG                                                                                <  SRR4302540.4624388/94‑1 (MQ=60)
                CTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGG                                                                               <  SRR4302540.8226092/94‑1 (MQ=60)
                CTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGG                                                                               >  SRR4302540.6149944/1‑94 (MQ=60)
                 TCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGC                                                                              >  SRR4302540.5368280/1‑94 (MQ=60)
                 TCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGGTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGC                                                                              >  SRR4302540.2327781/1‑94 (MQ=60)
                   CCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGA                                                                            >  SRR4302540.8553353/1‑94 (MQ=60)
                   CCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGA                                                                            >  SRR4302540.1079638/1‑94 (MQ=60)
                    CCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAA                                                                           <  SRR4302540.7414985/94‑1 (MQ=60)
                     CGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAA                                                                          >  SRR4302540.6363557/1‑94 (MQ=60)
                      GGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAAT                                                                         <  SRR4302540.6796993/94‑1 (MQ=60)
                      GGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAAT                                                                         <  SRR4302540.9102568/94‑1 (MQ=60)
                      GGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAAT                                                                         <  SRR4302540.3377971/94‑1 (MQ=60)
                       GTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATT                                                                        <  SRR4302540.5459830/94‑1 (MQ=60)
                       GTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATT                                                                        <  SRR4302540.2985122/94‑1 (MQ=60)
                       GTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATT                                                                        <  SRR4302540.5619611/94‑1 (MQ=60)
                       GTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATT                                                                        <  SRR4302540.5459829/94‑1 (MQ=60)
                       GTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATT                                                                        >  SRR4302540.2580940/1‑94 (MQ=60)
                       GTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATT                                                                        >  SRR4302540.2315822/1‑94 (MQ=60)
                        TTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGAGGCGAAATTA                                                                       >  SRR4302540.7387567/1‑94 (MQ=60)
                         TCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTAT                                                                      <  SRR4302540.2957536/94‑1 (MQ=60)
                         TCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTAT                                                                      >  SRR4302540.308990/1‑94 (MQ=60)
                          CCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATG                                                                     <  SRR4302540.7105605/94‑1 (MQ=60)
                           CCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGA                                                                    <  SRR4302540.5736489/94‑1 (MQ=60)
                           CCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGA                                                                    <  SRR4302540.8897138/94‑1 (MQ=60)
                           CCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGA                                                                    <  SRR4302540.4067809/94‑1 (MQ=60)
                           CCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGA                                                                    <  SRR4302540.4038174/94‑1 (MQ=60)
                            CGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGAT                                                                   <  SRR4302540.5320626/94‑1 (MQ=60)
                            CACATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGAT                                                                   >  SRR4302540.1283012/1‑94 (MQ=60)
                             GCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATT                                                                  >  SRR4302540.7621242/1‑94 (MQ=60)
                             GCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATT                                                                  >  SRR4302540.5351920/1‑94 (MQ=60)
                              CATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGcggaaataatattga                                                                 >  SRR4302540.5889061/1‑79 (MQ=60)
                               ATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGA                                                                >  SRR4302540.7489505/1‑94 (MQ=60)
                               ATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGA                                                                >  SRR4302540.6498645/1‑94 (MQ=60)
                               ATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGA                                                                >  SRR4302540.7489506/1‑94 (MQ=60)
                                 CAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATA                                                              <  SRR4302540.7541951/94‑1 (MQ=60)
                                  AGAATTACGTGGTAGTACGTTGCGCCTTGCTCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATAT                                                             <  SRR4302540.2626919/94‑1 (MQ=60)
                                  AGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATAT                                                             >  SRR4302540.6643676/1‑94 (MQ=60)
                                  AGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATAT                                                             >  SRR4302540.4351460/1‑94 (MQ=60)
                                  AGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATAT                                                             >  SRR4302540.2126402/1‑94 (MQ=60)
                                  AGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATAT                                                             >  SRR4302540.3641996/1‑94 (MQ=60)
                                  AGAATTACGTGGTAGTACGTTGCGCCTTGCGCAACTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATAT                                                             <  SRR4302540.9041738/94‑1 (MQ=60)
                                   GAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATA                                                            <  SRR4302540.3759049/94‑1 (MQ=60)
                                   GAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATA                                                            <  SRR4302540.2234709/94‑1 (MQ=60)
                                     ATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGACTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAA                                                          >  SRR4302540.1697521/1‑94 (MQ=60)
                                      TTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAAT                                                         >  SRR4302540.4855400/1‑94 (MQ=60)
                                      TTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAAT                                                         >  SRR4302540.5775758/1‑94 (MQ=60)
                                      TTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAAT                                                         <  SRR4302540.3859513/94‑1 (MQ=60)
                                       TACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATC                                                        <  SRR4302540.8323861/94‑1 (MQ=60)
                                       TACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATC                                                        <  SRR4302540.6213852/94‑1 (MQ=60)
                                       TACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATC                                                        <  SRR4302540.6931797/94‑1 (MQ=60)
                                        ACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCA                                                       <  SRR4302540.3900091/94‑1 (MQ=60)
                                        ACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCA                                                       <  SRR4302540.3197372/94‑1 (MQ=60)
                                        ACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCA                                                       <  SRR4302540.9341430/94‑1 (MQ=60)
                                         CGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCAC                                                      <  SRR4302540.8040540/94‑1 (MQ=60)
                                         CGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCAC                                                      <  SRR4302540.6067645/94‑1 (MQ=60)
                                         CGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCAC                                                      <  SRR4302540.9985121/94‑1 (MQ=60)
                                         CGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCAC                                                      <  SRR4302540.179875/94‑1 (MQ=60)
                                         CGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCAC                                                      <  SRR4302540.1879550/94‑1 (MQ=60)
                                         CGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCAC                                                      <  SRR4302540.1082846/94‑1 (MQ=60)
                                          GTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACG                                                     >  SRR4302540.9715475/1‑94 (MQ=60)
                                          GTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACG                                                     <  SRR4302540.1388940/94‑1 (MQ=60)
                                           TGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGC                                                    <  SRR4302540.7975257/94‑1 (MQ=60)
                                           TGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGC                                                    >  SRR4302540.7932797/1‑94 (MQ=60)
                                            GGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCC                                                   >  SRR4302540.5728437/1‑94 (MQ=60)
                                            GGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCC                                                   >  SRR4302540.2605439/1‑94 (MQ=60)
                                             GTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACACGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCT                                                  >  SRR4302540.6716455/1‑94 (MQ=60)
                                              TAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTA                                                 <  SRR4302540.9668632/94‑1 (MQ=60)
                                               AGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAA                                                <  SRR4302540.1418494/94‑1 (MQ=60)
                                               AGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAA                                                <  SRR4302540.6996561/94‑1 (MQ=60)
                                               AGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAA                                                <  SRR4302540.2009586/94‑1 (MQ=60)
                                                GTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGACATTATGATTGATATAAATCACGCCTAGG                                               >  SRR4302540.1797429/1‑94 (MQ=60)
                                                GTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAG                                               >  SRR4302540.2711839/1‑94 (MQ=60)
                                                GTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAG                                               <  SRR4302540.951853/94‑1 (MQ=60)
                                                GTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAG                                               >  SRR4302540.9675160/1‑94 (MQ=60)
                                                GTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAG                                               <  SRR4302540.5106986/94‑1 (MQ=60)
                                                  ACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCT                                             <  SRR4302540.4589286/94‑1 (MQ=60)
                                                  ACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCT                                             >  SRR4302540.1527943/1‑94 (MQ=60)
                                                   CGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGATT                                            >  SRR4302540.3195698/1‑94 (MQ=60)
                                                    GTTGCGCCTTGCGCATTTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTT                                           <  SRR4302540.8900626/94‑1 (MQ=60)
                                                    GTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTT                                           >  SRR4302540.6709669/1‑94 (MQ=60)
                                                    GTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTT                                           <  SRR4302540.8344784/94‑1 (MQ=60)
                                                    GTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTT                                           <  SRR4302540.1193654/94‑1 (MQ=60)
                                                    GTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTT                                           >  SRR4302540.4332605/1‑94 (MQ=60)
                                                    GTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACACGCTTCGCTTTCACTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTT                                           >  SRR4302540.4277164/1‑94 (MQ=60)
                                                     TTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTG                                          <  SRR4302540.4342334/94‑1 (MQ=60)
                                                     TTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTG                                          <  SRR4302540.6819400/94‑1 (MQ=60)
                                                     TTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTG                                          >  SRR4302540.6626511/1‑94 (MQ=60)
                                                      TGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGC                                         >  SRR4302540.3650701/1‑94 (MQ=60)
                                                        CGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCAC                                       <  SRR4302540.6430770/94‑1 (MQ=60)
                                                         GCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACA                                      >  SRR4302540.7568446/1‑94 (MQ=60)
                                                         GCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACA                                      >  SRR4302540.5509095/1‑94 (MQ=60)
                                                          CCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACAT                                     <  SRR4302540.7916657/94‑1 (MQ=60)
                                                          CCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACAT                                     <  SRR4302540.10081823/94‑1 (MQ=60)
                                                          CCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACAT                                     <  SRR4302540.6699994/94‑1 (MQ=60)
                                                           CTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACCTC                                    >  SRR4302540.1058207/1‑94 (MQ=60)
                                                           CTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATC                                    >  SRR4302540.1058208/1‑94 (MQ=60)
                                                           CTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATC                                    <  SRR4302540.8975008/94‑1 (MQ=60)
                                                            TTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCG                                   <  SRR4302540.6762264/94‑1 (MQ=60)
                                                            TTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCG                                   <  SRR4302540.439796/94‑1 (MQ=60)
                                                            TTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCG                                   >  SRR4302540.3307926/1‑94 (MQ=60)
                                                             TGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGT                                  <  SRR4302540.2301556/94‑1 (MQ=60)
                                                               CGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCC                                >  SRR4302540.4426838/1‑94 (MQ=60)
                                                               CGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCC                                <  SRR4302540.6885158/94‑1 (MQ=60)
                                                               CGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCC                                >  SRR4302540.5194406/1‑94 (MQ=60)
                                                                 CATCTGTTTCTTCATTTAATGCCTGGCGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCAT                              <  SRR4302540.4222033/94‑1 (MQ=60)
                                                                 CATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCAT                              >  SRR4302540.209366/1‑94 (MQ=60)
                                                                 CATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCAT                              <  SRR4302540.9481524/94‑1 (MQ=60)
                                                                  ATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATA                             >  SRR4302540.708307/1‑94 (MQ=60)
                                                                  ATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATA                             >  SRR4302540.8379179/1‑94 (MQ=60)
                                                                  ATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATA                             <  SRR4302540.7893545/94‑1 (MQ=60)
                                                                  ATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATA                             >  SRR4302540.6857163/1‑94 (MQ=60)
                                                                  ATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATA                             >  SRR4302540.2559345/1‑94 (MQ=60)
                                                                  ATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGACTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATA                             >  SRR4302540.7967299/1‑94 (MQ=60)
                                                                   TCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATAT                            <  SRR4302540.4404419/94‑1 (MQ=60)
                                                                   TCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATAT                            <  SRR4302540.2837776/94‑1 (MQ=60)
                                                                   TCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATAT                            >  SRR4302540.4192045/1‑94 (MQ=60)
                                                                   TCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATAT                            <  SRR4302540.3311040/94‑1 (MQ=60)
                                                                    CTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATT                           >  SRR4302540.3080406/1‑94 (MQ=60)
                                                                    CTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATT                           >  SRR4302540.8234320/1‑94 (MQ=60)
                                                                     TGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTT                          >  SRR4302540.6408381/1‑94 (MQ=60)
                                                                      GTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTC                         <  SRR4302540.1649705/94‑1 (MQ=60)
                                                                      GTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTC                         >  SRR4302540.6732539/1‑94 (MQ=60)
                                                                       TTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCT                        <  SRR4302540.10126836/94‑1 (MQ=60)
                                                                       TTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCT                        <  SRR4302540.5412601/94‑1 (MQ=60)
                                                                        TTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTG                       <  SRR4302540.6149986/94‑1 (MQ=60)
                                                                        TTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTG                       <  SRR4302540.2762961/94‑1 (MQ=60)
                                                                        TTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTG                       <  SRR4302540.1827503/94‑1 (MQ=60)
                                                                        TGCTTCATCTAATGCCTGACGCGCTTCGCGTTCAGGGGCGAAATTAGGATTGATATAAAGCACGCCTAAGCGTTGCACATCGTCCATATTTCTG                       <  SRR4302540.9867042/94‑1 (MQ=60)
                                                                          CTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGC                     <  SRR4302540.5235528/94‑1 (MQ=60)
                                                                          CTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGC                     >  SRR4302540.9635985/1‑94 (MQ=60)
                                                                          CTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGC                     >  SRR4302540.447774/1‑94 (MQ=60)
                                                                           TTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCC                    >  SRR4302540.6816953/1‑94 (MQ=60)
                                                                            TCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCT                   <  SRR4302540.214416/94‑1 (MQ=60)
                                                                            TCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCT                   <  SRR4302540.5579681/94‑1 (MQ=60)
                                                                            TCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTACGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCT                   <  SRR4302540.257275/94‑1 (MQ=60)
                                                                             CATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTG                  >  SRR4302540.8289323/1‑94 (MQ=60)
                                                                             CATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTG                  <  SRR4302540.8978746/94‑1 (MQ=60)
                                                                             CATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTG                  >  SRR4302540.6545547/1‑94 (MQ=60)
                                                                              ATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGG                 >  SRR4302540.2842207/1‑94 (MQ=60)
                                                                              ATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGG                 >  SRR4302540.7231890/1‑94 (MQ=60)
                                                                              ATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGG                 <  SRR4302540.2323514/94‑1 (MQ=60)
                                                                               TTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGT                <  SRR4302540.9146470/94‑1 (MQ=60)
                                                                                 TAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGG              >  SRR4302540.9744047/1‑94 (MQ=60)
                                                                                 TAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGG              <  SRR4302540.593361/94‑1 (MQ=60)
                                                                                  AATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGT             >  SRR4302540.2594193/1‑94 (MQ=60)
                                                                                   ATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTT            >  SRR4302540.9200345/1‑94 (MQ=60)
                                                                                   ATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTT            >  SRR4302540.9614049/1‑94 (MQ=60)
                                                                                    TGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTA           >  SRR4302540.2598313/1‑94 (MQ=60)
                                                                                     GCCTGACGCGCTTCGCTTTCAGTGGCTAAATTATGATTGATATAAATCACGCCTAAGCTTTGCTCATCGTCCATATTTCTGGCCTGGTGGTTAT          <  SRR4302540.8874580/94‑1 (MQ=60)
                                                                                     GCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTAT          <  SRR4302540.10127824/94‑1 (MQ=60)
                                                                                       CTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTA        <  SRR4302540.8966969/94‑1 (MQ=60)
                                                                                       CTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTA        <  SRR4302540.4133886/94‑1 (MQ=60)
                                                                                       CTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTA        >  SRR4302540.387486/1‑94 (MQ=60)
                                                                                        TGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAA       >  SRR4302540.3630796/1‑94 (MQ=60)
                                                                                        TGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAA       >  SRR4302540.537430/1‑94 (MQ=60)
                                                                                         GACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAAT      <  SRR4302540.4119593/94‑1 (MQ=60)
                                                                                         GACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAAT      <  SRR4302540.3137801/94‑1 (MQ=60)
                                                                                          ACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATT     <  SRR4302540.1149881/94‑1 (MQ=60)
                                                                                            GCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTC   >  SRR4302540.4093500/1‑94 (MQ=60)
                                                                                            GCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTC   <  SRR4302540.8542368/94‑1 (MQ=60)
                                                                                            GCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTC   <  SRR4302540.6965839/94‑1 (MQ=60)
                                                                                             CGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCA  <  SRR4302540.2509395/94‑1 (MQ=60)
                                                                                             CGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCA  >  SRR4302540.7320182/1‑94 (MQ=60)
                                                                                             CGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCA  >  SRR4302540.4679172/1‑94 (MQ=60)
                                                                                             |                                                                                             
GTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCA  >  NC_000913/4422118‑4422304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑