Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,422,211	T→C	A53A (GCA→GCG)	yjfY ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,422,211	0	T	C	99.2%	849.9 / ‑5.7	265	A53A (GCA→GCG)	yjfY	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (131/132); total (132/133)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 270 total aligned reads displayed.

CATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAAT  >  NC_000913/4422121‑4422300

cATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACgcgc                                                                                         <  1:4869253/93‑1 (MQ=255)

 atatTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCt                                                                                        <  2:4660144/93‑1 (MQ=255)

 atatTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCt                                                                                        >  1:198119/1‑93 (MQ=255)

  tatTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCtt                                                                                       >  2:6190737/1‑93 (MQ=255)

  tatTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCtt                                                                                       >  2:3108105/1‑93 (MQ=255)

    ttGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCg                                                                                     >  1:1721183/1‑93 (MQ=255)

     tGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGc                                                                                    <  2:732753/93‑1 (MQ=255)

      gCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCt                                                                                   <  1:2620008/93‑1 (MQ=255)

      gCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCt                                                                                   <  1:4358280/93‑1 (MQ=255)

      gCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCt                                                                                   <  1:3026399/93‑1 (MQ=255)

       ccGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGcgcttcgctt                                                                                  <  1:69154/93‑1 (MQ=255)

       ccGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGcgcttcgctt                                                                                  >  2:2806453/1‑93 (MQ=255)

         gTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTc                                                                                <  1:1032484/93‑1 (MQ=255)

          ttACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCtttta                                                                               <  2:5009883/93‑3 (MQ=255)

          ttACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCa                                                                               >  2:3761832/1‑93 (MQ=255)

          ttACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCa                                                                               <  1:516501/93‑1 (MQ=255)

          ttACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCa                                                                               >  2:1611746/1‑93 (MQ=255)

            aCTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGt                                                                             <  2:1866680/93‑1 (MQ=255)

            aCTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGt                                                                             <  1:499485/93‑1 (MQ=255)

             cTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTg                                                                            <  2:4020355/93‑1 (MQ=255)

              tCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTgg                                                                           <  1:2887969/93‑1 (MQ=255)

                    gTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAAt                                                                     >  2:3892820/1‑93 (MQ=255)

                    gTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAAt                                                                     >  1:1142359/1‑93 (MQ=255)

                     ttCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAAtt                                                                    >  2:1440295/1‑93 (MQ=255)

                     ttCCCACATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAAtt                                                                    <  2:6131859/93‑1 (MQ=255)

                      tCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTa                                                                   <  2:1053113/93‑1 (MQ=255)

                      tCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTa                                                                   >  1:4635903/1‑93 (MQ=255)

                      tCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTa                                                                   <  1:4246130/93‑1 (MQ=255)

                       cccGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTAt                                                                  <  2:432783/93‑1 (MQ=255)

                        ccGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTAtg                                                                 >  2:1662601/1‑93 (MQ=255)

                         cGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTAtga                                                                >  2:2564754/1‑93 (MQ=255)

                         cGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTAtga                                                                <  2:5901797/93‑1 (MQ=255)

                         cGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTAtga                                                                <  2:5600109/93‑1 (MQ=255)

                          gCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTAtgat                                                               >  2:3543701/1‑93 (MQ=255)

                          gCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTAtgat                                                               >  1:3224058/1‑93 (MQ=255)

                          gCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTAtgat                                                               >  1:928740/1‑93 (MQ=255)

                            aTCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTAtgattg                                                             >  1:2766902/1‑93 (MQ=255)

                             tCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTAtgattga                                                            >  1:5844733/1‑93 (MQ=255)

                              cAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTAtgattgat                                                           <  1:4468750/93‑1 (MQ=255)

                              cAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTAtgattgat                                                           <  1:5774045/93‑1 (MQ=255)

                                gAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGatat                                                         <  2:4195665/93‑1 (MQ=255)

                                gAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGatat                                                         >  1:1593958/1‑93 (MQ=255)

                                 aaTTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGAtata                                                        <  1:5183900/93‑1 (MQ=255)

                                 aaTTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGAtata                                                        >  2:4140056/1‑93 (MQ=255)

                                   ttACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATaaa                                                      <  2:4909014/93‑1 (MQ=255)

                                   ttACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATaaa                                                      <  2:1742243/93‑1 (MQ=255)

                                   ttACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATaaa                                                      <  1:2112762/93‑1 (MQ=255)

                                   ttACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATaaa                                                      <  2:1354873/93‑1 (MQ=255)

                                      cGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCa                                                   <  1:1928992/93‑1 (MQ=255)

                                      cGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCa                                                   >  2:459506/1‑93 (MQ=255)

                                      cGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCa                                                   <  1:5362904/93‑1 (MQ=255)

                                      cGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCa                                                   >  1:5921641/1‑93 (MQ=255)

                                       gTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCAc                                                  >  2:1211194/1‑93 (MQ=255)

                                       gTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCAc                                                  <  1:4760073/93‑1 (MQ=255)

                                        tGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACg                                                 <  1:6174080/93‑1 (MQ=255)

                                         ggTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGc                                                <  1:3717714/93‑1 (MQ=255)

                                         ggTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGc                                                >  1:4042564/1‑93 (MQ=255)

                                          gtagtaCGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGcc                                               >  1:3321915/1‑93 (MQ=255)

                                          gtagtaCGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGcc                                               <  2:1519390/93‑1 (MQ=255)

                                          gtagtaCGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGcc                                               <  2:2928272/93‑1 (MQ=255)

                                            agtaCGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTa                                             >  2:950581/1‑93 (MQ=255)

                                            agtaCGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTa                                             <  1:839910/93‑1 (MQ=255)

                                              taCGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAg                                           <  2:244781/93‑1 (MQ=255)

                                              taCGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAg                                           >  2:2748368/1‑93 (MQ=255)

                                                 gTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCtt                                        <  2:4446939/93‑1 (MQ=255)

                                                 gTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCtt                                        <  1:345878/93‑1 (MQ=255)

                                                 gTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCtt                                        >  2:5748274/1‑93 (MQ=255)

                                                  ttGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCttt                                       >  1:1902129/1‑93 (MQ=255)

                                                  ttGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCttt                                       >  1:6217443/1‑93 (MQ=255)

                                                   tGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTg                                      <  2:5865/93‑1 (MQ=255)

                                                       ccTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGcaca                                  >  2:2302236/1‑93 (MQ=255)

                                                       ccTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGcaca                                  <  1:4910834/93‑1 (MQ=255)

                                                       ccTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGcaca                                  <  2:2369073/93‑1 (MQ=255)

                                                        cTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACAt                                 <  2:5534730/93‑1 (MQ=255)

                                                         ttGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATc                                <  1:2287736/93‑1 (MQ=255)

                                                           gcgcATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGt                              <  1:2691363/93‑1 (MQ=255)

                                                           gcgcATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGt                              <  2:5054589/93‑1 (MQ=255)

                                                             gcATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTcc                            >  2:3354066/1‑93 (MQ=255)

                                                             gcATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTcc                            <  1:5652760/93‑1 (MQ=255)

                                                              cATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCa                           <  2:5426667/93‑1 (MQ=255)

                                                                tCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCata                         >  2:4792671/1‑93 (MQ=255)

                                                                tCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCata                         <  2:4403178/93‑1 (MQ=255)

                                                                 cTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCatat                        >  1:6136066/1‑93 (MQ=255)

                                                                 cTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCatat                        <  1:603642/93‑1 (MQ=255)

                                                                    tttCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTc                     <  2:3944449/93‑1 (MQ=255)

                                                                       cttcATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTgg                  >  1:5440114/1‑93 (MQ=255)

                                                                        ttcATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGc                 >  2:5110194/1‑93 (MQ=255)

                                                                         tcATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGcc                >  2:2680390/1‑93 (MQ=255)

                                                                         tcATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGcc                >  1:2523805/1‑93 (MQ=255)

                                                                          cATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCt               >  2:62707/1‑93 (MQ=255)

                                                                           aTTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCtg              <  2:1962470/93‑1 (MQ=255)

                                                                            tttAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCtgg             <  1:1261215/93‑1 (MQ=255)

                                                                             ttAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCtggt            <  1:3857845/93‑1 (MQ=255)

                                                                              tAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCtggtg           <  1:5264540/93‑1 (MQ=255)

                                                                                  gCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGtta       >  2:3196838/1‑93 (MQ=255)

                                                                                  gCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGtta       >  1:1579401/1‑93 (MQ=255)

                                                                                  gCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGtta       >  1:995458/1‑93 (MQ=255)

                                                                                    cTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGttatt     >  1:2200475/1‑93 (MQ=255)

                                                                                      gACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTaa   >  2:3276328/1‑93 (MQ=255)

                                                                                      gACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTaa   <  1:3572525/93‑1 (MQ=255)

CATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAAT  >  NC_000913/4422121‑4422300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCA  >  NC_000913/4422118‑4422304

GTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGC                                                                                               >  SRR4302533.5325598/1‑94 (MQ=60)

GTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGC                                                                                               >  SRR4302533.1508685/1‑94 (MQ=60)

GTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGC                                                                                               >  SRR4302533.2810410/1‑94 (MQ=60)

GTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTCAATGCCTGACGC                                                                                               >  SRR4302533.4715436/1‑94 (MQ=60)

 TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCG                                                                                              >  SRR4302533.2770723/1‑94 (MQ=60)

 TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGAAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTCATGACTGACGCG                                                                                              >  SRR4302533.2003816/1‑94 (MQ=60)

  GCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGC                                                                                             <  SRR4302533.5462361/94‑1 (MQ=60)

   CATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCT                                                                                            >  SRR4302533.199368/1‑94 (MQ=60)

   CATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCT                                                                                            <  SRR4302533.4898756/94‑1 (MQ=60)

     TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTC                                                                                          <  SRR4302533.6219963/94‑1 (MQ=60)

      ATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCG                                                                                         >  SRR4302533.371973/1‑94 (MQ=60)

      ATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCG                                                                                         <  SRR4302533.2187141/94‑1 (MQ=60)

      ATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCG                                                                                         >  SRR4302533.1731621/1‑94 (MQ=60)

       TTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGC                                                                                        <  SRR4302533.3496224/94‑1 (MQ=60)

         GCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTT                                                                                      <  SRR4302533.3044920/94‑1 (MQ=60)

         GCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTT                                                                                      <  SRR4302533.4384586/94‑1 (MQ=60)

         GCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTT                                                                                      <  SRR4302533.2636090/94‑1 (MQ=60)

         GCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTT                                                                                      >  SRR4302533.6198246/1‑94 (MQ=60)

         GCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTT                                                                                      <  SRR4302533.2083418/94‑1 (MQ=60)

          CCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTT                                                                                     <  SRR4302533.69606/94‑1 (MQ=60)

          CCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTT                                                                                     >  SRR4302533.978951/1‑94 (MQ=60)

            GTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCA                                                                                   <  SRR4302533.1038556/94‑1 (MQ=60)

            GTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCA                                                                                   <  SRR4302533.2741868/94‑1 (MQ=60)

             TTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAG                                                                                  <  SRR4302533.1953254/94‑1 (MQ=60)

             TTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAG                                                                                  <  SRR4302533.519566/94‑1 (MQ=60)

              TACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGT                                                                                 <  SRR4302533.2817311/94‑1 (MQ=60)

              TACTCCCCGGTTCCCGCATCAGAATTACGT‑GTAGTACCGTGCGGCTTGCGGATCTGGTTCTTCCTTTTATTGCTGACGCGCTTCGCCTTCAGTG                                                                                >  SRR4302533.4144532/1‑94 (MQ=19)

               ACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTG                                                                                <  SRR4302533.502459/94‑1 (MQ=60)

               ACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTG                                                                                <  SRR4302533.2612095/94‑1 (MQ=60)

                CTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGG                                                                               <  SRR4302533.1176506/94‑1 (MQ=60)

                CTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGG                                                                               >  SRR4302533.5613064/1‑94 (MQ=60)

                CTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGG                                                                               <  SRR4302533.126577/94‑1 (MQ=60)

                CTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGG                                                                               <  SRR4302533.2369198/94‑1 (MQ=60)

                 TCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGC                                                                              <  SRR4302533.1047888/94‑1 (MQ=60)

                 TCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGC                                                                              <  SRR4302533.2905722/94‑1 (MQ=60)

                   CCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGA                                                                            >  SRR4302533.4668103/1‑94 (MQ=60)

                     CGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAA                                                                          >  SRR4302533.1680818/1‑94 (MQ=60)

                      GGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAAT                                                                         >  SRR4302533.1149169/1‑94 (MQ=60)

                      GGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAAT                                                                         >  SRR4302533.3325732/1‑94 (MQ=60)

                      GGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAAT                                                                         >  SRR4302533.4361304/1‑94 (MQ=60)

                      GGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAAT                                                                         >  SRR4302533.5224549/1‑94 (MQ=60)

                      GGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAAT                                                                         >  SRR4302533.604511/1‑94 (MQ=60)

                      CGTTCCCGCATCAGAATTACGGGGTAGCACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAAT                                                                         <  SRR4302533.4161906/94‑1 (MQ=60)

                       GTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATT                                                                        >  SRR4302533.927830/1‑94 (MQ=60)

                        TTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTA                                                                       >  SRR4302533.4663991/1‑94 (MQ=60)

                         TCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTAT                                                                      >  SRR4302533.4537508/1‑94 (MQ=60)

                         TCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTAT                                                                      <  SRR4302533.4271762/94‑1 (MQ=60)

                          CCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATG                                                                     >  SRR4302533.591440/1‑94 (MQ=60)

                           CCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGA                                                                    <  SRR4302533.6289929/94‑1 (MQ=60)

                            CGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGAT                                                                   >  SRR4302533.934193/1‑94 (MQ=60)

                            CGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGAT                                                                   <  SRR4302533.6221138/94‑1 (MQ=60)

                            CGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGAT                                                                   <  SRR4302533.5763102/94‑1 (MQ=60)

                            CGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGAT                                                                   >  SRR4302533.3243937/1‑94 (MQ=60)

                             GCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATT                                                                  <  SRR4302533.3137062/94‑1 (MQ=60)

                              CATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTG                                                                 >  SRR4302533.2783874/1‑94 (MQ=60)

                               ATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGA                                                                >  SRR4302533.5880068/1‑94 (MQ=60)

                               ATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGA                                                                >  SRR4302533.1753192/1‑94 (MQ=60)

                                TCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGAT                                                               <  SRR4302533.2282700/94‑1 (MQ=60)

                                TCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGAT                                                               <  SRR4302533.4418459/94‑1 (MQ=60)

                                TCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGAT                                                               <  SRR4302533.1372242/94‑1 (MQ=60)

                                TCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGAT                                                               >  SRR4302533.6152653/1‑94 (MQ=60)

                                 CAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATA                                                              <  SRR4302533.4495781/94‑1 (MQ=60)

                                 CAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATA                                                              <  SRR4302533.5292217/94‑1 (MQ=60)

                                 CAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATA                                                              >  SRR4302533.5715261/1‑94 (MQ=60)

                                 CAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATA                                                              <  SRR4302533.5808917/94‑1 (MQ=60)

                                  AGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATAT                                                             >  SRR4302533.3651823/1‑94 (MQ=60)

                                  AGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATAT                                                             >  SRR4302533.1603602/1‑94 (MQ=60)

                                    AATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAA                                                           <  SRR4302533.5215194/94‑1 (MQ=60)

                                      TTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAAT                                                         <  SRR4302533.2125795/94‑1 (MQ=60)

                                      TTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAAT                                                         <  SRR4302533.5187480/94‑1 (MQ=60)

                                       TACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATC                                                        >  SRR4302533.4717084/1‑94 (MQ=60)

                                        ACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCA                                                       >  SRR4302533.5957435/1‑94 (MQ=60)

                                         CGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCAC                                                      <  SRR4302533.4349444/94‑1 (MQ=60)

                                         CGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCAC                                                      <  SRR4302533.1940763/94‑1 (MQ=60)

                                         CGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCAC                                                      <  SRR4302533.5395112/94‑1 (MQ=60)

                                         CGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCAC                                                      >  SRR4302533.3457168/1‑94 (MQ=60)

                                          GTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACG                                                     <  SRR4302533.4788950/94‑1 (MQ=60)

                                           TGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGC                                                    <  SRR4302533.6211183/94‑1 (MQ=60)

                                           TGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGC                                                    >  SRR4302533.4067169/1‑94 (MQ=60)

                                            GGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCC                                                   >  SRR4302533.3342433/1‑94 (MQ=60)

                                            GGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCC                                                   <  SRR4302533.3740533/94‑1 (MQ=60)

                                             GTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCT                                                  <  SRR4302533.2915112/94‑1 (MQ=60)

                                             GTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCT                                                  <  SRR4302533.3196784/94‑1 (MQ=60)

                                               AGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAA                                                <  SRR4302533.844823/94‑1 (MQ=60)

                                               AGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAA                                                >  SRR4302533.2153837/1‑94 (MQ=60)

                                                GTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAG                                               <  SRR4302533.1981323/94‑1 (MQ=60)

                                                GTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAG                                               >  SRR4302533.6030436/1‑94 (MQ=60)

                                                 TACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGC                                              <  SRR4302533.5201456/94‑1 (MQ=60)

                                                   CGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTT                                            >  SRR4302533.3858442/1‑94 (MQ=60)

                                                    GTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTT                                           >  SRR4302533.6254724/1‑94 (MQ=60)

                                                    GTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTT                                           >  SRR4302533.3864654/1‑94 (MQ=60)

                                                    GTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTT                                           <  SRR4302533.347990/94‑1 (MQ=60)

                                                    GTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTT                                           >  SRR4302533.1913968/1‑94 (MQ=60)

                                                    GTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTT                                           >  SRR4302533.1913740/1‑94 (MQ=60)

                                                      TGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGC                                         <  SRR4302533.2681546/94‑1 (MQ=60)

                                                      TGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGC                                         >  SRR4302533.498050/1‑94 (MQ=60)

                                                        CGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCAC                                       >  SRR4302533.3324573/1‑94 (MQ=60)

                                                          CCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACAT                                     <  SRR4302533.4940585/94‑1 (MQ=60)

                                                          CCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACAT                                     >  SRR4302533.5394032/1‑94 (MQ=60)

                                                            TTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCG                                   <  SRR4302533.2301896/94‑1 (MQ=60)

                                                            TTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCG                                   <  SRR4302533.583353/94‑1 (MQ=60)

                                                              GCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTC                                 >  SRR4302533.5073121/1‑94 (MQ=60)

                                                              GCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTC                                 <  SRR4302533.2707859/94‑1 (MQ=60)

                                                               CGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCC                                >  SRR4302533.2781797/1‑94 (MQ=60)

                                                                GCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCA                               <  SRR4302533.5686926/94‑1 (MQ=60)

                                                                GCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCA                               >  SRR4302533.901158/1‑94 (MQ=60)

                                                                 CATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCAT                              >  SRR4302533.4596585/1‑94 (MQ=60)

                                                                  ATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATA                             >  SRR4302533.5363892/1‑94 (MQ=60)

                                                                   TCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATAT                            <  SRR4302533.417481/94‑1 (MQ=60)

                                                                   TCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATAT                            >  SRR4302533.6173035/1‑94 (MQ=60)

                                                                   TCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATAT                            >  SRR4302533.6127714/1‑94 (MQ=60)

                                                                   TCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATAT                            >  SRR4302533.4940641/1‑94 (MQ=60)

                                                                   TCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATAT                            <  SRR4302533.2595146/94‑1 (MQ=60)

                                                                    CTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATT                           <  SRR4302533.607188/94‑1 (MQ=60)

                                                                      GTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTC                         >  SRR4302533.2850153/1‑94 (MQ=60)

                                                                      GTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTC                         <  SRR4302533.2054089/94‑1 (MQ=60)

                                                                       TTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCT                        <  SRR4302533.6318119/94‑1 (MQ=60)

                                                                        TTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTG                       >  SRR4302533.944462/1‑94 (MQ=60)

                                                                         TCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGG                      >  SRR4302533.5472875/1‑94 (MQ=60)

                                                                         TCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGC                      >  SRR4302533.4907251/1‑94 (MQ=60)

                                                                         TCTTCATTTAATGCCTGACGCGCTTCGCTTCAAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGG                      <  SRR4302533.764212/94‑1 (MQ=60)

                                                                          CTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGC                     >  SRR4302533.5872443/1‑94 (MQ=60)

                                                                           TTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCC                    >  SRR4302533.180018/1‑94 (MQ=60)

                                                                           TTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCC                    >  SRR4302533.2539351/1‑94 (MQ=60)

                                                                            TCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCT                   <  SRR4302533.5409208/94‑1 (MQ=60)

                                                                             CATTTAATGCCTGACGCGTTTCGTTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTG                  <  SRR4302533.3923267/94‑1 (MQ=60)

                                                                               TTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGT                >  SRR4302533.1372033/1‑94 (MQ=60)

                                                                               TTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGT                <  SRR4302533.1268757/94‑1 (MQ=60)

                                                                                TTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTG               <  SRR4302533.3881401/94‑1 (MQ=60)

                                                                                TTAAAGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTG               <  SRR4302533.3436519/94‑1 (MQ=60)

                                                                                 TAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGG              <  SRR4302533.5296136/94‑1 (MQ=60)

                                                                                    TGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTA           >  SRR4302533.1001272/1‑94 (MQ=60)

                                                                                    TGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTA           >  SRR4302533.1588915/1‑94 (MQ=60)

                                                                                     GCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTAT          >  SRR4302533.4789041/1‑94 (MQ=60)

                                                                                      CCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATT         >  SRR4302533.2214032/1‑94 (MQ=60)

                                                                                       CTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTA        <  SRR4302533.5522618/94‑1 (MQ=60)

                                                                                       CTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTA        <  SRR4302533.2472154/94‑1 (MQ=60)

                                                                                       CTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTA        >  SRR4302533.1592497/1‑94 (MQ=60)

                                                                                        TGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAA       >  SRR4302533.1627709/1‑94 (MQ=60)

                                                                                         GACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAAT      <  SRR4302533.3594537/94‑1 (MQ=60)

                                                                                          ACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATT     >  SRR4302533.1198539/1‑94 (MQ=60)

                                                                                          ACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATT     <  SRR4302533.487258/94‑1 (MQ=60)

                                                                                             CGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCA  >  SRR4302533.525198/1‑94 (MQ=60)

                                                                                             CGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCA  >  SRR4302533.4521945/1‑94 (MQ=60)

                                                                                             CGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCA  >  SRR4302533.3708591/1‑94 (MQ=60)

GTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCA  >  NC_000913/4422118‑4422304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`