Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,441,383 T→C S475S (AGT→AGC)  ytfM → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,441,3830TC99.7% 1171.4 / ‑5.2 362S475S (AGT→AGC) ytfMhypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (140/221);  total (140/222)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.48e-01

Only 100 of 377 total aligned reads displayed.

TGTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGGA  >  NC_000913/4441293‑4441472
                                                                                           |                                                                                         
 gTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCAtt                                                                                        <  2:5646327/93‑1 (MQ=255)
   tACACGCGGCACGCTGTGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCg                                                                                      <  1:4331884/93‑1 (MQ=255)
    acacGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCgg                                                                                     >  2:5512187/1‑92 (MQ=255)
    acacGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGt                                                                                     <  1:6667844/93‑1 (MQ=255)
    acacGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGt                                                                                     <  2:8487136/93‑1 (MQ=255)
       cgcgGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGGCGACCGCAGCATTCGTgg                                                                                   <  2:9198941/93‑1 (MQ=255)
       cgcgGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGc                                                                                  <  2:4114942/93‑1 (MQ=255)
       cgcgGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGc                                                                                  <  1:9164146/93‑1 (MQ=255)
       cgcgGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGc                                                                                  <  2:5157814/93‑1 (MQ=255)
       cgcgGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGc                                                                                  <  2:9356366/93‑1 (MQ=255)
        gcgGGACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCt                                                                                 <  1:688465/93‑1 (MQ=255)
        gcgGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCt                                                                                 <  1:2897100/93‑1 (MQ=255)
         cgGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCta                                                                                <  1:2699649/93‑1 (MQ=255)
          ggCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCtac                                                                               <  1:6188253/93‑1 (MQ=255)
           gCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCtaca                                                                              <  1:4589766/93‑1 (MQ=255)
           gCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCtaca                                                                              <  2:7286302/93‑1 (MQ=255)
            cACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCtacaa                                                                             <  2:2204573/93‑1 (MQ=255)
             aCGCTGGGCTGGATTGAACCCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCtacaaa                                                                            <  1:3961909/93‑1 (MQ=255)
              cGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCtacaaat                                                                           <  1:7436007/93‑1 (MQ=255)
                 tgggctggATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCtacaaataca                                                                        <  2:1506520/93‑1 (MQ=255)
                 tgggctggATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGGGGCtccaaataca                                                                        >  2:8727802/1‑93 (MQ=255)
                  gggctggATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCtacaaatacaa                                                                       >  2:2444795/1‑93 (MQ=255)
                  gggctggATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCtacaaatacaa                                                                       >  2:3013016/1‑93 (MQ=255)
                  gggctcgATTTAAACCGGTGATTTCGACATAGAACGGCCGGATCTGCGATTGCTCCCC‑GGGGGCGACCGCAGCACTGGCGGCtacaaatacaa                                                                       >  2:7352741/1‑93 (MQ=255)
                   ggctggATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCtacaaatacaaa                                                                      <  2:6856590/93‑1 (MQ=255)
                    gctggATTGAAACCGGTGATTTCGACAAAGTACCGCCTGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTacaaatacaaat                                                                     >  2:7904907/1‑93 (MQ=255)
                        gATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTAt                                                                 <  1:1382669/93‑1 (MQ=255)
                         aTTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATCCAAATCTATc                                                                <  2:80064/93‑1 (MQ=255)
                          ttGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCg                                                               >  2:2900230/1‑93 (MQ=255)
                          ttGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCg                                                               >  1:5998079/1‑93 (MQ=255)
                           tGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGc                                                              <  1:1117466/93‑1 (MQ=255)
                           tGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGc                                                              <  2:8260971/93‑1 (MQ=255)
                             aaaCCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTc                                                            <  1:943039/93‑1 (MQ=255)
                              aaCCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACACATCTATCGCTcc                                                           >  2:5215600/1‑93 (MQ=255)
                              aaCCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTcc                                                           <  2:3776313/93‑1 (MQ=255)
                                    tGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATa                                                     <  1:5117345/93‑1 (MQ=255)
                                     gATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATAc                                                    <  1:7087890/93‑1 (MQ=255)
                                     gATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATAc                                                    >  2:2153609/1‑93 (MQ=255)
                                      aTTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACg                                                   <  2:3852575/93‑1 (MQ=255)
                                      aTTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACg                                                   >  2:6530115/1‑93 (MQ=255)
                                        ttCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGcc                                                 <  2:8665450/93‑1 (MQ=255)
                                          cGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCaa                                               <  2:7773027/93‑1 (MQ=255)
                                          cGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCaa                                               <  1:4801607/93‑1 (MQ=255)
                                          cGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCaa                                               <  2:5279865/93‑1 (MQ=255)
                                           gACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAAc                                              <  2:3999477/93‑1 (MQ=255)
                                           gACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAAc                                              <  2:1294614/93‑1 (MQ=255)
                                           gACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAAc                                              <  2:7192787/93‑1 (MQ=255)
                                             cAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACgg                                            <  1:8915731/93‑1 (MQ=255)
                                             cAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACgg                                            >  2:2001923/1‑93 (MQ=255)
                                              aaaGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGt                                           >  2:7001985/1‑93 (MQ=255)
                                              aaaGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGt                                           <  1:6138831/93‑1 (MQ=255)
                                               aaGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCGACGGTg                                          >  2:7519137/1‑93 (MQ=255)
                                                aGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGa                                         <  1:4142176/93‑1 (MQ=255)
                                                 gTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGAc                                        <  2:6716719/93‑1 (MQ=255)
                                                  tACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGAcc                                       >  1:6780033/1‑93 (MQ=255)
                                                       ccgGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGaaa                                  <  1:8202724/93‑1 (MQ=255)
                                                       ccgGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGaaa                                  <  2:4846346/93‑1 (MQ=255)
                                                       ccgGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGaaa                                  <  2:4352662/93‑1 (MQ=255)
                                                       ccgGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGaaa                                  >  1:5621848/1‑93 (MQ=255)
                                                       ccgGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGaaa                                  >  2:10001337/1‑93 (MQ=255)
                                                        cgGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAg                                 <  1:728563/93‑1 (MQ=255)
                                                         ggATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAgg                                <  2:1596019/93‑1 (MQ=255)
                                                         ggATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAgg                                >  2:3650880/1‑93 (MQ=255)
                                                          gATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAggg                               <  2:4758976/93‑1 (MQ=255)
                                                          gATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAggg                               >  2:1751827/1‑93 (MQ=255)
                                                          gATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAggg                               >  2:7120992/1‑93 (MQ=255)
                                                          gATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAggg                               >  1:4560596/1‑93 (MQ=255)
                                                           aTCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAgggg                              >  1:4698627/1‑93 (MQ=255)
                                                            tCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGc                             >  2:2579392/1‑93 (MQ=255)
                                                            acTGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGc                             <  2:6275244/92‑1 (MQ=255)
                                                              tGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCt                           >  1:2831323/1‑93 (MQ=255)
                                                              tGCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCt                           <  1:1451394/93‑1 (MQ=255)
                                                               tcGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTc                          <  2:6939621/92‑1 (MQ=255)
                                                               gCGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTc                          <  1:7680536/93‑1 (MQ=255)
                                                                cGTTTCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCg                         <  1:6401242/93‑1 (MQ=255)
                                                                  tttCTTCGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGaa                       >  2:277259/1‑93 (MQ=255)
                                                                   ttcttcGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAg                      >  1:5194546/1‑93 (MQ=255)
                                                                   ttcttcGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAg                      <  1:2436445/93‑1 (MQ=255)
                                                                    tcttcGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGt                     >  1:9867551/1‑93 (MQ=255)
                                                                      ttcGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTg                   >  1:10025514/1‑93 (MQ=255)
                                                                       tcGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGa                  >  1:3412655/1‑93 (MQ=255)
                                                                        cGCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGaa                 >  1:8541554/1‑92 (MQ=255)
                                                                         gCC‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATa                >  1:5828769/1‑93 (MQ=255)
                                                                          gc‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATaa               <  2:3250677/92‑1 (MQ=255)
                                                                          cc‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAATGGGGCCTCGAAGTTGATaa               <  2:3557970/93‑1 (MQ=255)
                                                                          cc‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATaa               <  1:1299808/93‑1 (MQ=255)
                                                                          cc‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATaa               <  2:4994801/93‑1 (MQ=255)
                                                                           c‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAAc              <  1:7020338/93‑1 (MQ=255)
                                                                           c‑GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAAc              <  1:8137046/93‑1 (MQ=255)
                                                                              ggggCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCg            >  1:6363921/1‑93 (MQ=255)
                                                                                ggCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGa          <  2:3342539/93‑1 (MQ=255)
                                                                                ggCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGa          >  1:7523016/1‑93 (MQ=255)
                                                                                  cGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATc        <  1:3071048/93‑1 (MQ=255)
                                                                                    aCCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCgt      >  1:3204596/1‑92 (MQ=255)
                                                                                    aCCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGc      >  2:5835788/1‑93 (MQ=255)
                                                                                    aCCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGc      <  2:5349222/93‑1 (MQ=255)
                                                                                     ccGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCt     >  1:3808702/1‑93 (MQ=255)
                                                                                       gcagcaTTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTgg   >  1:2045596/1‑93 (MQ=255)
                                                                                       gcagcaTTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTgg   >  2:9866583/1‑93 (MQ=255)
                                                                                        cAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGGa  >  2:8799117/1‑93 (MQ=255)
                                                                                           |                                                                                         
TGTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCC‑GGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGGA  >  NC_000913/4441293‑4441472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTTGTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGGAATAC  >  NC_000913/4441290‑4441476
                                                                                             |                                                                                             
TTTTGTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGC                                                                                               <  SRR4302540.5906265/94‑1 (MQ=60)
TTTTGTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGC                                                                                               <  SRR4302540.948886/94‑1 (MQ=60)
 TTTGTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCA                                                                                              >  SRR4302540.8025413/1‑94 (MQ=60)
  TTGTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCAT                                                                                             <  SRR4302540.7839442/94‑1 (MQ=60)
  TTGTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCAT                                                                                             <  SRR4302540.8724343/94‑1 (MQ=60)
   TGTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATT                                                                                            >  SRR4302540.1394183/1‑94 (MQ=60)
    TTTACGCGCGGCACGCTGGGCTGGATTGAAACCGGTGAGTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTC                                                                                           <  SRR4302540.6092068/94‑1 (MQ=60)
    GTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTC                                                                                           <  SRR4302540.52502/94‑1 (MQ=60)
    GTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTC                                                                                           <  SRR4302540.3096505/94‑1 (MQ=60)
    GTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTC                                                                                           <  SRR4302540.6092067/94‑1 (MQ=60)
      TACACGCGGCACGCTGTGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGT                                                                                         <  SRR4302540.4379037/94‑1 (MQ=60)
      TACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGT                                                                                         <  SRR4302540.1321631/94‑1 (MQ=60)
      TACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGT                                                                                         <  SRR4302540.217656/94‑1 (MQ=60)
      TACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGT                                                                                         <  SRR4302540.644189/94‑1 (MQ=60)
       ACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTG                                                                                        >  SRR4302540.1186772/1‑94 (MQ=60)
       ACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTG                                                                                        <  SRR4302540.6741539/94‑1 (MQ=60)
        CACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGG                                                                                       <  SRR4302540.6387070/94‑1 (MQ=60)
         ACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGC                                                                                      <  SRR4302540.7716417/94‑1 (MQ=60)
          CGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCT                                                                                     <  SRR4302540.9274112/94‑1 (MQ=60)
          CGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCT                                                                                     >  SRR4302540.2870415/1‑94 (MQ=60)
           GCGGGACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTA                                                                                    <  SRR4302540.695853/94‑1 (MQ=60)
           GCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTA                                                                                    <  SRR4302540.2928699/94‑1 (MQ=60)
           GCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTA                                                                                    <  SRR4302540.4405215/94‑1 (MQ=60)
            CGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTAC                                                                                   <  SRR4302540.4077561/94‑1 (MQ=60)
            CGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTAC                                                                                   >  SRR4302540.6400562/1‑94 (MQ=60)
            CGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTAC                                                                                   <  SRR4302540.2728787/94‑1 (MQ=60)
             GGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACA                                                                                  <  SRR4302540.6257157/94‑1 (MQ=60)
             GGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACA                                                                                  >  SRR4302540.1430772/1‑94 (MQ=60)
              GCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAA                                                                                 <  SRR4302540.4639176/94‑1 (MQ=60)
              GCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAA                                                                                 <  SRR4302540.4639175/94‑1 (MQ=60)
               GACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAA                                                                                <  SRR4302540.3962600/94‑1 (MQ=60)
               CACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAA                                                                                <  SRR4302540.7569454/94‑1 (MQ=60)
                ccgtcggggTGGATTGAAACCGGTTATTTGGAAAAAGTGCCGCCGGATCTGCGGTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAAT                                                                               <  SRR4302540.6987261/85‑1 (MQ=60)
                ACGCTGGGCTGGATTGAACCCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAAT                                                                               <  SRR4302540.4004635/94‑1 (MQ=60)
                ACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAAT                                                                               <  SRR4302540.6987262/94‑1 (MQ=60)
                ACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAAT                                                                               <  SRR4302540.7785397/94‑1 (MQ=60)
                ACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAAT                                                                               <  SRR4302540.2096157/94‑1 (MQ=60)
                ACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAAT                                                                               <  SRR4302540.9139530/94‑1 (MQ=60)
                 CGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATA                                                                              <  SRR4302540.7518861/94‑1 (MQ=60)
                 CGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGCGGCGACCGCAGCATTCGTGGCTACAAATC                                                                              >  SRR4302540.6326930/1‑94 (MQ=60)
                   CTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACA                                                                            <  SRR4302540.5139629/94‑1 (MQ=60)
                   CTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACA                                                                            <  SRR4302540.1059363/94‑1 (MQ=60)
                   CTGGGCTGGATGGAAACCGGTGATTACGACAAAGTACCGCCGGATCTGCGTTTCTGCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACA                                                                            <  SRR4302540.3867842/94‑1 (MQ=60)
                    TGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAA                                                                           >  SRR4302540.4427687/1‑94 (MQ=60)
                      GGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAAT                                                                         >  SRR4302540.7073890/1‑94 (MQ=60)
                       GCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATC                                                                        <  SRR4302540.428121/94‑1 (MQ=60)
                       GCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATC                                                                        <  SRR4302540.3340623/94‑1 (MQ=60)
                          GGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTAT                                                                     >  SRR4302540.6953943/1‑94 (MQ=60)
                           GATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATC                                                                    <  SRR4302540.8172261/94‑1 (MQ=60)
                           GATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATC                                                                    <  SRR4302540.1397429/94‑1 (MQ=60)
                           GATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATC                                                                    <  SRR4302540.1391171/94‑1 (MQ=60)
                            ATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCG                                                                   <  SRR4302540.9969289/94‑1 (MQ=60)
                            ATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCG                                                                   >  SRR4302540.6064551/1‑94 (MQ=60)
                            ATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCC                                                                   >  SRR4302540.6064550/1‑94 (MQ=60)
                             TTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGC                                                                  >  SRR4302540.7232374/1‑94 (MQ=60)
                             TTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGC                                                                  <  SRR4302540.2801871/94‑1 (MQ=60)
                             ATGAACCCGGTGATTTGGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGC                                                                  <  SRR4302540.2801870/94‑1 (MQ=60)
                              TGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCT                                                                 >  SRR4302540.5809730/1‑94 (MQ=60)
                              TGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCT                                                                 <  SRR4302540.1129055/94‑1 (MQ=60)
                                AAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCC                                                               <  SRR4302540.4028223/94‑1 (MQ=60)
                                AAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCC                                                               <  SRR4302540.952881/94‑1 (MQ=60)
                                AAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCC                                                               <  SRR4302540.7546706/94‑1 (MQ=60)
                                 AGCCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCG                                                              <  SRR4302540.3107461/94‑1 (MQ=60)
                                 AACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCG                                                              <  SRR4302540.7719881/94‑1 (MQ=60)
                                    CGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAA                                                           >  SRR4302540.2689259/1‑94 (MQ=60)
                                    CGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAA                                                           >  SRR4302540.4359730/1‑94 (MQ=60)
                                     GGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAAT                                                          <  SRR4302540.2739730/94‑1 (MQ=60)
                                     GGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAAT                                                          <  SRR4302540.3641416/94‑1 (MQ=60)
                                      GTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATA                                                         <  SRR4302540.645403/94‑1 (MQ=60)
                                      GTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATA                                                         >  SRR4302540.654810/1‑94 (MQ=60)
                                       TGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATAC                                                        <  SRR4302540.7155065/94‑1 (MQ=60)
                                       TGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATAC                                                        <  SRR4302540.5892213/94‑1 (MQ=60)
                                       TGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATAC                                                        <  SRR4302540.987692/94‑1 (MQ=60)
                                       TGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATAC                                                        <  SRR4302540.5172747/94‑1 (MQ=60)
                                       TGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATAC                                                        <  SRR4302540.5172746/94‑1 (MQ=60)
                                        GATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACG                                                       <  SRR4302540.7492968/94‑1 (MQ=60)
                                        GATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACG                                                       <  SRR4302540.7166763/94‑1 (MQ=60)
                                        GATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACG                                                       <  SRR4302540.7492967/94‑1 (MQ=60)
                                        GATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACG                                                       <  SRR4302540.7079090/94‑1 (MQ=60)
                                         ATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGC                                                      <  SRR4302540.579582/94‑1 (MQ=60)
                                          TTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCC                                                     <  SRR4302540.6829235/94‑1 (MQ=60)
                                           TTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCA                                                    >  SRR4302540.9359573/1‑94 (MQ=60)
                                            TCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAA                                                   >  SRR4302540.5185794/1‑94 (MQ=60)
                                             CGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAAC                                                  <  SRR4302540.4414385/94‑1 (MQ=60)
                                             CGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAAC                                                  <  SRR4302540.759200/94‑1 (MQ=60)
                                             CGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAAC                                                  <  SRR4302540.4852992/94‑1 (MQ=60)
                                             CGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAAC                                                  <  SRR4302540.3467165/94‑1 (MQ=60)
                                             CGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAAC                                                  >  SRR4302540.4696825/1‑94 (MQ=60)
                                             CGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAAC                                                  <  SRR4302540.9763990/94‑1 (MQ=60)
                                              GACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACG                                                 <  SRR4302540.7879909/94‑1 (MQ=60)
                                              GACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACG                                                 <  SRR4302540.5596878/94‑1 (MQ=60)
                                                CAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGT                                               <  SRR4302540.9018986/94‑1 (MQ=60)
                                                 AAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTG                                              <  SRR4302540.3363801/94‑1 (MQ=60)
                                                 AAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTG                                              <  SRR4302540.6207201/94‑1 (MQ=60)
                                                   AGTGCGGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGAC                                            <  SRR4302540.4186993/94‑1 (MQ=60)
                                                   AGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGAC                                            <  SRR4302540.2394027/94‑1 (MQ=60)
                                                   AGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGAC                                            <  SRR4302540.4186995/94‑1 (MQ=60)
                                                    GTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACC                                           >  SRR4302540.6854994/1‑94 (MQ=60)
                                                     TACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCT                                          <  SRR4302540.3833176/94‑1 (MQ=60)
                                                      ACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTG                                         >  SRR4302540.4827949/1‑94 (MQ=60)
                                                        CGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAA                                       >  SRR4302540.8335562/1‑94 (MQ=60)
                                                         GCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAA                                      >  SRR4302540.6221665/1‑94 (MQ=60)
                                                         GCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAA                                      <  SRR4302540.8757649/94‑1 (MQ=60)
                                                         GCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAA                                      >  SRR4302540.4626372/1‑94 (MQ=60)
                                                         GCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAA                                      >  SRR4302540.5683704/1‑94 (MQ=60)
                                                         GCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCCTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAA                                      >  SRR4302540.6221666/1‑94 (MQ=60)
                                                          CCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAG                                     >  SRR4302540.8257318/1‑94 (MQ=60)
                                                          CCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAG                                     <  SRR4302540.8298045/94‑1 (MQ=60)
                                                          CCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAG                                     <  SRR4302540.3156703/94‑1 (MQ=60)
                                                          CCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAG                                     <  SRR4302540.4796250/94‑1 (MQ=60)
                                                           CGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGG                                    <  SRR4302540.1412330/94‑1 (MQ=60)
                                                           CGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGG                                    <  SRR4302540.736364/94‑1 (MQ=60)
                                                            GGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGG                                   >  SRR4302540.4609873/1‑94 (MQ=60)
                                                            GGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGG                                   <  SRR4302540.3090060/94‑1 (MQ=60)
                                                            GGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGG                                   >  SRR4302540.8913107/1‑94 (MQ=60)
                                                             GATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGG                                  >  SRR4302540.4748854/1‑94 (MQ=60)
                                                             GATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGG                                  <  SRR4302540.5132486/94‑1 (MQ=60)
                                                             GATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGG                                  >  SRR4302540.4754646/1‑94 (MQ=60)
                                                              ATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGC                                 >  SRR4302540.3168571/1‑94 (MQ=60)
                                                              ATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGC                                 >  SRR4302540.4184352/1‑94 (MQ=60)
                                                              ATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGC                                 >  SRR4302540.5587078/1‑94 (MQ=60)
                                                               TCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCC                                >  SRR4302540.8693318/1‑94 (MQ=60)
                                                                cttccTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAGCGGTGACCTGAAAGGGGCCT                               <  SRR4302540.5115481/89‑1 (MQ=60)
                                                                CTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCT                               >  SRR4302540.2861945/1‑94 (MQ=60)
                                                                CTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCT                               >  SRR4302540.1123340/1‑94 (MQ=60)
                                                                 TGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTC                              <  SRR4302540.1467175/94‑1 (MQ=60)
                                                                  GCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCG                             <  SRR4302540.658165/94‑1 (MQ=60)
                                                                  GCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCG                             <  SRR4302540.4369120/94‑1 (MQ=60)
                                                                  GCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCG                             <  SRR4302540.7767470/94‑1 (MQ=60)
                                                                   CGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGA                            <  SRR4302540.2709449/94‑1 (MQ=60)
                                                                   CGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGA                            <  SRR4302540.6472491/94‑1 (MQ=60)
                                                                   CGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGA                            >  SRR4302540.6861465/1‑94 (MQ=60)
                                                                   CGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAAGCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGA                            <  SRR4302540.6472490/94‑1 (MQ=60)
                                                                    GTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAA                           <  SRR4302540.7432124/94‑1 (MQ=60)
                                                                    GTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAA                           >  SRR4302540.903358/1‑94 (MQ=60)
                                                                    GTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAA                           >  SRR4302540.10085143/1‑94 (MQ=60)
                                                                    GTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAA                           >  SRR4302540.712357/1‑94 (MQ=60)
                                                                    GTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAA                           >  SRR4302540.50926/1‑94 (MQ=60)
                                                                     TTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAG                          >  SRR4302540.3441198/1‑94 (MQ=60)
                                                                     TTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAG                          >  SRR4302540.5251111/1‑94 (MQ=60)
                                                                      TTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGT                         >  SRR4302540.9982809/1‑94 (MQ=60)
                                                                      TTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGT                         >  SRR4302540.9982808/1‑94 (MQ=60)
                                                                      TTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGT                         <  SRR4302540.1258199/94‑1 (MQ=60)
                                                                      TTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGT                         <  SRR4302540.2462686/94‑1 (MQ=60)
                                                                      TTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGT                         >  SRR4302540.6533506/1‑94 (MQ=60)
                                                                      TTCTTCGCCGGGGGCGACCGCAGCATTCGCGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGT                         >  SRR4302540.1474186/1‑94 (MQ=60)
                                                                        CTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTG                       >  SRR4302540.10142169/1‑94 (MQ=60)
                                                                         TTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGA                      >  SRR4302540.3449814/1‑94 (MQ=60)
                                                                         TTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGA                      >  SRR4302540.5900947/1‑94 (MQ=60)
                                                                         TTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGA                      <  SRR4302540.9526125/94‑1 (MQ=60)
                                                                         TTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGA                      <  SRR4302540.6835533/94‑1 (MQ=60)
                                                                          TCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGAT                     <  SRR4302540.3016043/94‑1 (MQ=60)
                                                                          TCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGAA                     >  SRR4302540.8640797/1‑94 (MQ=60)
                                                                           CGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATA                    >  SRR4302540.5893207/1‑94 (MQ=60)
                                                                           CGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATA                    <  SRR4302540.1766838/94‑1 (MQ=60)
                                                                             CCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAAC                  <  SRR4302540.1313280/94‑1 (MQ=60)
                                                                             CCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAAC                  <  SRR4302540.1313281/94‑1 (MQ=60)
                                                                              CGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACC                 <  SRR4302540.8284027/94‑1 (MQ=60)
                                                                              CGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACC                 <  SRR4302540.8231490/94‑1 (MQ=60)
                                                                              CGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACC                 <  SRR4302540.7098311/94‑1 (MQ=60)
                                                                              CGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACC                 >  SRR4302540.2891109/1‑94 (MQ=60)
                                                                              CGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACC                 <  SRR4302540.7098310/94‑1 (MQ=60)
                                                                               GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCG                >  SRR4302540.1451268/1‑94 (MQ=60)
                                                                               GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCG                >  SRR4302540.6434782/1‑94 (MQ=60)
                                                                               GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCG                >  SRR4302540.836532/1‑94 (MQ=60)
                                                                               GGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCG                >  SRR4302540.9043755/1‑94 (MQ=60)
                                                                                 GGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGA              >  SRR4302540.7607181/1‑94 (MQ=60)
                                                                                 GGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGA              >  SRR4302540.7607172/1‑94 (MQ=60)
                                                                                  GGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGAT             >  SRR4302540.4622147/1‑94 (MQ=60)
                                                                                   GCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATC            <  SRR4302540.8393083/94‑1 (MQ=60)
                                                                                    CGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCG           >  SRR4302540.10015595/1‑94 (MQ=60)
                                                                                    CGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCG           <  SRR4302540.3104745/94‑1 (MQ=60)
                                                                                    CGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCG           <  SRR4302540.4722201/94‑1 (MQ=60)
                                                                                     GACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGT          >  SRR4302540.3239398/1‑94 (MQ=60)
                                                                                      ACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCT         >  SRR4302540.3850182/1‑94 (MQ=60)
                                                                                       CCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTG        >  SRR4302540.6203256/1‑94 (MQ=60)
                                                                                        CGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGG       >  SRR4302540.2067632/1‑94 (MQ=60)
                                                                                        CGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGG       >  SRR4302540.7335402/1‑94 (MQ=60)
                                                                                        CGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGG       >  SRR4302540.6020632/1‑94 (MQ=60)
                                                                                         GCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGGA      >  SRR4302540.257387/1‑94 (MQ=60)
                                                                                          CAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGGAA     <  SRR4302540.6913280/94‑1 (MQ=60)
                                                                                           AGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGCAAGGGGCCTCGAAGTTGATAACCGGATCGCTGGAAT    >  SRR4302540.4947475/1‑94 (MQ=60)
                                                                                           AGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGGAAT    >  SRR4302540.4947474/1‑94 (MQ=60)
                                                                                           AGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGGAAT    <  SRR4302540.6960780/94‑1 (MQ=60)
                                                                                            GCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGGAATA   >  SRR4302540.3046853/1‑94 (MQ=60)
                                                                                            GCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGGAATA   <  SRR4302540.4357589/94‑1 (MQ=60)
                                                                                             CATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGGAATAC  >  SRR4302540.3496638/1‑94 (MQ=60)
                                                                                             |                                                                                             
TTTTGTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGGAATAC  >  NC_000913/4441290‑4441476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑