Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,472,602 A→G Y56C (TAC→TGC)  b4252 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,472,6020AG99.4% 1673.6 / ‑2.6 528Y56C (TAC→TGC) b4252hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base G (271/254);  total (272/256)
Fisher's exact test for biased strand distribution p-value = 6.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.25e-01

Only 100 of 553 total aligned reads displayed.

CACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTACGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAAC  >  NC_000913/4472512‑4472691
                                                                                          |                                                                                         
  cacGTTACGGCAGCACCGCCAACGGGCAGGCACGATATCGACGGCGATCGACTGTTTTATCTTCTCTCGGAAGATATGACCGAGCCGTGCGag                                                                                       <  1:8984450/93‑2 (MQ=255)
  cacGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGGGCGaa                                                                                       >  1:3357944/1‑93 (MQ=255)
    cGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGc                                                                                     <  2:3351254/93‑1 (MQ=255)
     gtgaCGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCt                                                                                    <  2:360652/90‑1 (MQ=255)
     gTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCt                                                                                    >  2:7272971/1‑93 (MQ=255)
     gTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCt                                                                                    >  1:5286582/1‑93 (MQ=255)
        aCGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGc                                                                                 <  1:1351100/93‑1 (MQ=255)
           gCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGt                                                                              <  2:7844366/93‑1 (MQ=255)
             aGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGc                                                                            <  2:9961342/93‑1 (MQ=255)
             aGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGc                                                                            <  1:8297787/93‑1 (MQ=255)
              gAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCGGTGCGAAGCTCGCGGTGCg                                                                           >  1:3467641/1‑93 (MQ=255)
              gAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCg                                                                           >  2:5088111/1‑93 (MQ=255)
              gAAACGCCAAACGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCg                                                                           >  2:8267428/1‑93 (MQ=255)
                 aCGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAg                                                                        <  2:5764038/93‑1 (MQ=255)
                 aCGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAg                                                                        <  1:654453/93‑1 (MQ=255)
                  cGCCAAAGGGCAAGCACGATCTCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGt                                                                       <  2:5397428/93‑1 (MQ=255)
                   gCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGATCGCCGTGCGGAGTa                                                                      >  2:1739910/1‑93 (MQ=255)
                      aaaGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCa                                                                   <  2:3106323/93‑1 (MQ=255)
                      aaaGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGCAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCa                                                                   <  1:6929520/93‑1 (MQ=255)
                         gggCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGc                                                                <  2:8316648/93‑1 (MQ=255)
                          ggCAAGCACGATATCGAAGGCAATGGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGcc                                                               <  2:3984987/93‑1 (MQ=255)
                          ggCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGcc                                                               >  2:4743921/1‑93 (MQ=255)
                          ggCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGcc                                                               <  2:9647282/93‑1 (MQ=255)
                             aaGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGc                                                            <  2:4561909/93‑1 (MQ=255)
                             aaGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTACGAAGCTCGCCGTGCGGAGTACCATGCCCGc                                                            <  2:1849922/93‑1 (MQ=255)
                              aGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCt                                                           >  1:5511967/1‑93 (MQ=255)
                              aGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCt                                                           >  2:6153210/1‑93 (MQ=255)
                              aGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCt                                                           <  2:2555890/93‑1 (MQ=255)
                                 aCGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATc                                                        >  1:3826066/1‑93 (MQ=255)
                                 aCGATATCGAAGGCAATCGACTGTTCCATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCTGCTATc                                                        >  2:8968560/1‑93 (MQ=255)
                                  cGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGACCGCTATCt                                                       >  1:4732114/1‑93 (MQ=255)
                                  cGATATCGAAGGCAATCGACTGTTTTATCATATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCt                                                       >  1:2694613/1‑93 (MQ=255)
                                   gATATCGAAGGTAATCGACTGTTTTATCTTATCACGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCtt                                                      <  2:6282238/93‑1 (MQ=255)
                                   gATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCtt                                                      >  1:9966023/1‑93 (MQ=255)
                                    atatCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTg                                                     >  2:9065283/1‑93 (MQ=255)
                                    atatCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTg                                                     >  2:9175881/1‑93 (MQ=255)
                                     tatCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGa                                                    <  1:7165628/93‑1 (MQ=255)
                                     tatCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGa                                                    >  2:2281426/1‑93 (MQ=255)
                                       tCGAAGCCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACa                                                  <  1:8089820/93‑1 (MQ=255)
                                         gAAGGCAATCGACTGTTTTATCTGATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACAtt                                                <  2:5532111/93‑1 (MQ=255)
                                          aaGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTc                                               <  2:2742930/93‑1 (MQ=255)
                                           aGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCa                                              >  1:9162020/1‑93 (MQ=255)
                                           aGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCa                                              <  2:3812970/93‑1 (MQ=255)
                                            ggCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAg                                             >  1:10029434/1‑93 (MQ=255)
                                              agaTCGGCTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGAt                                           <  2:5828976/91‑1 (MQ=255)
                                               aaTCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGAtt                                          <  2:7607014/93‑1 (MQ=255)
                                                 tCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATtgt                                        >  1:3703857/1‑93 (MQ=255)
                                                 tCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATtgt                                        >  1:4546818/1‑93 (MQ=255)
                                                 tCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATtgt                                        <  2:6638710/93‑1 (MQ=255)
                                                  cGACTGTTTTATCTTAACTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATtgtg                                       <  1:3047018/93‑1 (MQ=255)
                                                    aCTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGtt                                     <  1:7671662/93‑1 (MQ=255)
                                                      tGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCTTGCCCGCTATCTTGACATTCAGATTGTGTTaa                                   >  1:5962657/1‑93 (MQ=255)
                                                      tGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTaa                                   >  2:8756897/1‑93 (MQ=255)
                                                      tGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTaa                                   >  1:1870241/1‑93 (MQ=255)
                                                       gTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTaaa                                  <  2:3267820/93‑1 (MQ=255)
                                                       gTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTaaa                                  >  1:5852854/1‑93 (MQ=255)
                                                        ttttATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTaaaa                                 <  1:6398581/93‑1 (MQ=255)
                                                        ttttATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTaaaa                                 >  1:3961578/1‑93 (MQ=255)
                                                         tttATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAg                                >  2:6778709/1‑93 (MQ=255)
                                                         tttATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAg                                <  2:9401587/93‑1 (MQ=255)
                                                         tttATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAg                                >  1:2159228/1‑93 (MQ=255)
                                                         tttATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAg                                <  1:1518887/93‑1 (MQ=255)
                                                         tttATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAg                                >  1:62353/1‑93 (MQ=255)
                                                         tttATCCTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTTTCTTGACATTCAGATTGTGTTAAAAg                                <  1:317118/93‑1 (MQ=255)
                                                          ttatcttatcTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAgg                               <  1:4444156/93‑1 (MQ=255)
                                                          ttatcttatcTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAgg                               <  1:4184075/93‑1 (MQ=255)
                                                           tatcttatctCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGt                              >  2:4479501/1‑93 (MQ=255)
                                                            atcttatctCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTc                             <  2:1168493/93‑1 (MQ=255)
                                                            atcttatctCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTc                             <  2:8406824/93‑1 (MQ=255)
                                                             tcttatctCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCa                            <  1:2024978/93‑1 (MQ=255)
                                                             tcttatctCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCa                            <  2:5319643/93‑1 (MQ=255)
                                                             tcttatctCGGAAGATATGACCGAGCCGCGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCa                            >  1:4995142/1‑93 (MQ=255)
                                                              cttatctCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAg                           >  1:9451258/1‑93 (MQ=255)
                                                                tatctCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGa                         <  2:6502148/93‑1 (MQ=255)
                                                                 atctCGGAAGATATGCCCGACCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGaa                        <  2:5271702/93‑1 (MQ=255)
                                                                 atctCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGaa                        <  1:6823490/93‑1 (MQ=255)
                                                                    tcGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGc                     <  1:2842939/93‑1 (MQ=255)
                                                                    tcGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGc                     >  2:4840506/1‑93 (MQ=255)
                                                                    tcGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGc                     >  1:7962724/1‑93 (MQ=255)
                                                                     cGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCa                    <  2:9775986/93‑1 (MQ=255)
                                                                       gAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGGCATTCAGATTGTGTTAAAAGGACAGGAAGGCGTg                  >  1:869514/1‑93 (MQ=255)
                                                                       gAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATg                  <  1:961622/93‑1 (MQ=255)
                                                                       gAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATg                  >  1:8851249/1‑93 (MQ=255)
                                                                         aGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGAc                >  2:1556116/1‑93 (MQ=255)
                                                                         aGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGAc                >  2:7496302/1‑93 (MQ=255)
                                                                          gATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGAcc               >  1:6116391/1‑93 (MQ=255)
                                                                           atatGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCt              >  2:4300198/1‑93 (MQ=255)
                                                                           atatGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCt              <  1:2433089/93‑1 (MQ=255)
                                                                             atGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTc            >  1:1604081/1‑93 (MQ=255)
                                                                               ggccGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAg          <  1:7445/91‑1 (MQ=255)
                                                                                aCCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGc         >  2:7694301/1‑93 (MQ=255)
                                                                                 ccGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCa        <  2:2438990/93‑1 (MQ=255)
                                                                                 ccGAGCCGTGCGAAGCTCGCCATGCGGAGGACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGTGCAGGAAGGCATGACCTTCAGcc        >  2:6954966/1‑92 (MQ=255)
                                                                                   gAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACg      <  2:7997771/93‑1 (MQ=255)
                                                                                    aGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGc     <  1:8461121/93‑1 (MQ=255)
                                                                                     gCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCa    <  2:4232860/93‑1 (MQ=255)
                                                                                     gCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCa    <  2:8500946/93‑1 (MQ=255)
                                                                                     gCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCa    >  1:1170644/1‑93 (MQ=255)
                                                                                      ccGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCaa   >  1:5412857/1‑93 (MQ=255)
                                                                                       cGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAAc  <  1:2246605/93‑1 (MQ=255)
                                                                                          |                                                                                         
CACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTACGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAAC  >  NC_000913/4472512‑4472691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 289 total aligned reads displayed.
AAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTACGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTG  >  NC_000913/4472509‑4472694
                                                                                             |                                                                                            
 AGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGC                                                                                             >  SRR4302540.9570183/1‑94 (MQ=60)
 AGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGC                                                                                             <  SRR4302540.9561347/94‑1 (MQ=60)
  GCACACGTTACGGCAGAAACGCCAAAGTGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCG                                                                                            <  SRR4302540.4338744/94‑1 (MQ=60)
  GCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCG                                                                                            <  SRR4302540.7934611/94‑1 (MQ=60)
  GCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCG                                                                                            <  SRR4302540.8305186/94‑1 (MQ=60)
   CACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGA                                                                                           <  SRR4302540.9437560/94‑1 (MQ=60)
   CACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGA                                                                                           >  SRR4302540.6924590/1‑94 (MQ=60)
    CCACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAA                                                                                          <  SRR4302540.3070769/94‑1 (MQ=60)
    ACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAA                                                                                          >  SRR4302540.7870390/1‑94 (MQ=60)
    ACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAA                                                                                          >  SRR4302540.9132646/1‑94 (MQ=60)
    ACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAA                                                                                          >  SRR4302540.7841313/1‑94 (MQ=60)
     CACGTTACGGCAGCACCGCCAACGGGCAGGCACGATATCGACGGCGATCGACTGTTTTATCTTCTCTCGGAAGATATGACCGAGCCGTGCGAGG                                                                                         <  SRR4302540.9091700/94‑1 (MQ=60)
     CACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAG                                                                                         <  SRR4302540.9016061/94‑1 (MQ=60)
     CACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAG                                                                                         >  SRR4302540.2615596/1‑94 (MQ=60)
       CGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGTCCGAGCCGTGCGAAGCT                                                                                       >  SRR4302540.9057889/1‑94 (MQ=60)
        GTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTC                                                                                      >  SRR4302540.6566995/1‑94 (MQ=60)
         TTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCG                                                                                     >  SRR4302540.9592508/1‑94 (MQ=60)
           ACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCG                                                                                   >  SRR4302540.8263189/1‑94 (MQ=60)
             GGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGT                                                                                 <  SRR4302540.251791/94‑1 (MQ=60)
             GGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGT                                                                                 >  SRR4302540.317177/1‑94 (MQ=60)
              GCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTG                                                                                <  SRR4302540.6744077/94‑1 (MQ=60)
                AGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCGGTGCGAAGCTCGCGGTGCG                                                                              >  SRR4302540.3505395/1‑94 (MQ=60)
                AGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCG                                                                              >  SRR4302540.7448357/1‑94 (MQ=60)
                AGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCG                                                                              >  SRR4302540.219726/1‑94 (MQ=60)
                AGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCG                                                                              >  SRR4302540.1851784/1‑94 (MQ=60)
                AGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCG                                                                              <  SRR4302540.8394135/94‑1 (MQ=60)
                AGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCG                                                                              >  SRR4302540.3141635/1‑94 (MQ=60)
                AGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCG                                                                              >  SRR4302540.4932548/1‑94 (MQ=60)
                AGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGAGCG                                                                              >  SRR4302540.1594901/1‑94 (MQ=60)
                 GAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGG                                                                             <  SRR4302540.5411446/94‑1 (MQ=60)
                   AACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAG                                                                           <  SRR4302540.9904428/94‑1 (MQ=60)
                    ACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGT                                                                          <  SRR4302540.1617594/94‑1 (MQ=60)
                       GCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACC                                                                       <  SRR4302540.8692671/94‑1 (MQ=60)
                       CCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACC                                                                       >  SRR4302540.7823278/1‑94 (MQ=60)
                       CCAAAGGGCAAGCACGATATCGAAGGAAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTGGCCGTGCGGAGTACA                                                                       >  SRR4302540.5879378/1‑94 (MQ=60)
                        CAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCA                                                                      >  SRR4302540.213744/1‑94 (MQ=60)
                        CAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCA                                                                      >  SRR4302540.2420891/1‑94 (MQ=60)
                        CAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCA                                                                      >  SRR4302540.5772140/1‑94 (MQ=60)
                         aaagtgtaatgctgatttcgaatgaaagctgttgtgTTATCTTATGTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCAT                                                                     <  SRR4302540.8415765/58‑1 (MQ=60)
                         AAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCAT                                                                     <  SRR4302540.1059950/94‑1 (MQ=60)
                         AAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCAT                                                                     <  SRR4302540.8415764/94‑1 (MQ=60)
                         AAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCAT                                                                     <  SRR4302540.5705845/94‑1 (MQ=60)
                         AAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGCAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCAT                                                                     <  SRR4302540.7006633/94‑1 (MQ=60)
                          AAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATG                                                                    <  SRR4302540.3456434/94‑1 (MQ=60)
                            GGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCC                                                                  >  SRR4302540.352785/1‑94 (MQ=60)
                            GGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCC                                                                  >  SRR4302540.1998922/1‑94 (MQ=60)
                             GGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCC                                                                 <  SRR4302540.5726208/94‑1 (MQ=60)
                              GCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCG                                                                <  SRR4302540.5446027/94‑1 (MQ=60)
                                AAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCT                                                              >  SRR4302540.5572673/1‑94 (MQ=60)
                                AAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCT                                                              >  SRR4302540.7517522/1‑94 (MQ=60)
                                AAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCT                                                              <  SRR4302540.2191475/94‑1 (MQ=60)
                                AAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCCCCGTGCGGAGTACCATGCCCGCT                                                              >  SRR4302540.5666951/1‑94 (MQ=60)
                                  GCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTAT                                                            <  SRR4302540.1275271/94‑1 (MQ=60)
                                   CACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATC                                                           >  SRR4302540.2279602/1‑94 (MQ=60)
                                   CACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATC                                                           >  SRR4302540.3867701/1‑94 (MQ=60)
                                   CACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGAGCGAAGCTCGCCGCGCGGCGTACCATGCCCGCCATC                                                           >  SRR4302540.8953194/1‑94 (MQ=60)
                                    ACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCT                                                          >  SRR4302540.6268176/1‑94 (MQ=60)
                                    ACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCT                                                          >  SRR4302540.2885233/1‑94 (MQ=60)
                                    ACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGACCGCTATCT                                                          >  SRR4302540.4782651/1‑94 (MQ=60)
                                    ACGATATCGAAGGCAATCGACTGTTTTATCATATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCT                                                          >  SRR4302540.2723704/1‑94 (MQ=60)
                                     CGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTT                                                         <  SRR4302540.8179999/94‑1 (MQ=60)
                                     CGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTT                                                         >  SRR4302540.10082297/1‑94 (MQ=60)
                                     CGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTT                                                         >  SRR4302540.5458635/1‑94 (MQ=60)
                                      GATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTG                                                        <  SRR4302540.3473326/94‑1 (MQ=60)
                                      GATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTG                                                        >  SRR4302540.5253103/1‑94 (MQ=60)
                                      GATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTG                                                        >  SRR4302540.3322808/1‑94 (MQ=60)
                                       ATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGA                                                       >  SRR4302540.9291503/1‑94 (MQ=60)
                                       ATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAACATCGCCGTGCGCAGTAACATGCCCGCTATCTTGA                                                       >  SRR4302540.3500011/1‑94 (MQ=60)
                                        TATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCTGCCGAAGCCCGCCGGCCGGGGCACCATGCCCGCTATCTTGca                                                      >  SRR4302540.968047/1‑92 (MQ=60)
                                        TATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGAC                                                      <  SRR4302540.1581043/94‑1 (MQ=60)
                                        TATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGAC                                                      <  SRR4302540.7245517/94‑1 (MQ=60)
                                        TATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGAC                                                      >  SRR4302540.4252224/1‑94 (MQ=60)
                                         ATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGATCGCCGGGCGGAGTACCATGCCCGCTATCTTGACA                                                     >  SRR4302540.3224898/1‑94 (MQ=60)
                                          TCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACAT                                                    <  SRR4302540.551823/94‑1 (MQ=60)
                                          TCGAAGCCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACAT                                                    <  SRR4302540.8183718/94‑1 (MQ=60)
                                           CGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCTCGCTATCTTGACATT                                                   <  SRR4302540.6661618/94‑1 (MQ=60)
                                           CGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATT                                                   >  SRR4302540.8669042/1‑94 (MQ=60)
                                           CGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATT                                                   >  SRR4302540.716205/1‑94 (MQ=60)
                                           CGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATT                                                   <  SRR4302540.6661617/94‑1 (MQ=60)
                                           CGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATT                                                   >  SRR4302540.1140975/1‑94 (MQ=60)
                                            GAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTC                                                  >  SRR4302540.2724652/1‑94 (MQ=60)
                                             AAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCA                                                 >  SRR4302540.279016/1‑94 (MQ=60)
                                             AAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCA                                                 >  SRR4302540.9271977/1‑94 (MQ=60)
                                             AAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCA                                                 <  SRR4302540.1227556/94‑1 (MQ=60)
                                              AGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAG                                                >  SRR4302540.10146111/1‑94 (MQ=60)
                                               GGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGA                                               >  SRR4302540.3993835/1‑94 (MQ=60)
                                                GCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGAT                                              <  SRR4302540.6370904/94‑1 (MQ=60)
                                                GCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGAT                                              >  SRR4302540.3844110/1‑94 (MQ=60)
                                                 CAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATT                                             <  SRR4302540.2287288/94‑1 (MQ=60)
                                                 CAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATT                                             >  SRR4302540.6961746/1‑94 (MQ=60)
                                                 CAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATT                                             >  SRR4302540.2136714/1‑94 (MQ=60)
                                                 CAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTACCTTGACATTCAGATT                                             >  SRR4302540.1528032/1‑94 (MQ=60)
                                                  AATCGACTTTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTG                                            <  SRR4302540.8165742/94‑1 (MQ=60)
                                                  AATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGGCCGCTATCTTGAAATTCAtaatt                                            >  SRR4302540.9691105/1‑89 (MQ=60)
                                                  AATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTG                                            >  SRR4302540.9740855/1‑94 (MQ=60)
                                                  AATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTG                                            >  SRR4302540.6389360/1‑94 (MQ=60)
                                                  AATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTG                                            >  SRR4302540.4012445/1‑94 (MQ=60)
                                                  AATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTG                                            <  SRR4302540.7501508/94‑1 (MQ=60)
                                                  AATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTG                                            <  SRR4302540.1714542/94‑1 (MQ=60)
                                                   ATCGACTTGTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGT                                           <  SRR4302540.2155075/94‑1 (MQ=60)
                                                   ATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGT                                           >  SRR4302540.6182318/1‑94 (MQ=60)
                                                   ATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGT                                           >  SRR4302540.4596009/1‑94 (MQ=60)
                                                   ATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGT                                           >  SRR4302540.3744292/1‑94 (MQ=60)
                                                    TCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTG                                          >  SRR4302540.5377113/1‑94 (MQ=60)
                                                     CGACTGTTTTATCTTATCTCGGAAGATATGCCCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGT                                         <  SRR4302540.9036369/94‑1 (MQ=60)
                                                     CGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGT                                         >  SRR4302540.504412/1‑94 (MQ=60)
                                                     CGACTGTTTTATCTTAACTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGT                                         <  SRR4302540.3080456/94‑1 (MQ=60)
                                                      GACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTT                                        >  SRR4302540.5528787/1‑94 (MQ=60)
                                                      GACTGTTTTATCTTATCTCGGAAGATATGAACGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTT                                        >  SRR4302540.6105957/1‑94 (MQ=60)
                                                       ACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTA                                       <  SRR4302540.7758221/94‑1 (MQ=60)
                                                       ACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTA                                       <  SRR4302540.7758222/94‑1 (MQ=60)
                                                       ACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTA                                       <  SRR4302540.3357983/94‑1 (MQ=60)
                                                       ACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCGTGACATTCAGATTGTGTTA                                       <  SRR4302540.4793083/94‑1 (MQ=60)
                                                        CTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCTTGCCCGCTATCTTGACATTCAGATTGTGTTAA                                      >  SRR4302540.6028628/1‑94 (MQ=60)
                                                        CTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAA                                      >  SRR4302540.1890736/1‑94 (MQ=60)
                                                        CTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAA                                      >  SRR4302540.6028629/1‑94 (MQ=60)
                                                         TGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAA                                     >  SRR4302540.9352160/1‑94 (MQ=60)
                                                         TGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAA                                     >  SRR4302540.4629925/1‑94 (MQ=60)
                                                         TGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAA                                     >  SRR4302540.5917587/1‑94 (MQ=60)
                                                           TTTTATCTTATCTCGGAAGATATGACCGAGCTGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAG                                   <  SRR4302540.5083214/94‑1 (MQ=60)
                                                           TTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAG                                   <  SRR4302540.4383693/94‑1 (MQ=60)
                                                           TTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAG                                   <  SRR4302540.6469814/94‑1 (MQ=60)
                                                           TTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAG                                   <  SRR4302540.8736725/94‑1 (MQ=60)
                                                           TTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAG                                   <  SRR4302540.8463879/94‑1 (MQ=60)
                                                           TTTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAG                                   <  SRR4302540.3247140/94‑1 (MQ=60)
                                                            TTTATCTTATCTCGGAAGCTATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAGAAGG                                  >  SRR4302540.7101775/1‑94 (MQ=60)
                                                            TTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAACAGG                                  >  SRR4302540.4104812/1‑94 (MQ=60)
                                                            TTTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGG                                  <  SRR4302540.1535693/94‑1 (MQ=60)
                                                            TTTATCCTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTTTCTTGACATTCAGATTGTGTTAAAAGG                                  <  SRR4302540.320282/94‑1 (MQ=60)
                                                             TTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGT                                 >  SRR4302540.6303331/1‑94 (MQ=60)
                                                             TTATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGT                                 >  SRR4302540.4513229/1‑94 (MQ=60)
                                                             TTATCTTATCTCGGAAGATATGACCGAGCCGTGCAAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGT                                 >  SRR4302540.6303581/1‑94 (MQ=60)
                                                              TATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTC                                >  SRR4302540.6553777/1‑94 (MQ=60)
                                                               ATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCA                               <  SRR4302540.4553421/94‑1 (MQ=60)
                                                               ATCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCA                               <  SRR4302540.4223896/94‑1 (MQ=60)
                                                               ATCTTATATCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGcta                               >  SRR4302540.9050867/1‑91 (MQ=60)
                                                                TCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAG                              <  SRR4302540.8172195/94‑1 (MQ=60)
                                                                TCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAG                              <  SRR4302540.2046857/94‑1 (MQ=60)
                                                                TCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAG                              >  SRR4302540.1407399/1‑94 (MQ=60)
                                                                TCTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAG                              >  SRR4302540.4278890/1‑94 (MQ=60)
                                                                 CTTTTCTCGGAAGATATGGCCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGG                             <  SRR4302540.2899107/94‑1 (MQ=60)
                                                                 CTTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGG                             <  SRR4302540.2773227/94‑1 (MQ=60)
                                                                  TTATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGA                            >  SRR4302540.8933387/1‑94 (MQ=60)
                                                                   TATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAA                           >  SRR4302540.824100/1‑94 (MQ=60)
                                                                   TATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAA                           >  SRR4302540.3704757/1‑94 (MQ=60)
                                                                   TATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAA                           >  SRR4302540.6486776/1‑94 (MQ=60)
                                                                    ATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAG                          >  SRR4302540.5938259/1‑94 (MQ=60)
                                                                    ATCTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAG                          <  SRR4302540.2676165/94‑1 (MQ=60)
                                                                      CTCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGC                        >  SRR4302540.6468264/1‑94 (MQ=60)
                                                                       TCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCA                       >  SRR4302540.8029008/1‑94 (MQ=60)
                                                                       TCGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCA                       >  SRR4302540.2038182/1‑94 (MQ=60)
                                                                        CGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACGATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCAT                      <  SRR4302540.171065/94‑1 (MQ=60)
                                                                        CGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCAT                      >  SRR4302540.9793657/1‑94 (MQ=60)
                                                                        CGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCAT                      <  SRR4302540.4546233/94‑1 (MQ=60)
                                                                        CGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCAT                      <  SRR4302540.225670/94‑1 (MQ=60)
                                                                        CGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCAT                      >  SRR4302540.4092689/1‑94 (MQ=60)
                                                                        CGGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCAT                      >  SRR4302540.1267062/1‑94 (MQ=60)
                                                                         GGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGGCATTCAGATTGTGTTAAAAGGACAGGAAGGCGTG                     >  SRR4302540.878742/1‑94 (MQ=60)
                                                                         GGAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATG                     <  SRR4302540.8906695/94‑1 (MQ=60)
                                                                          GAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGA                    <  SRR4302540.3750505/94‑1 (MQ=60)
                                                                          GAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGA                    >  SRR4302540.6906761/1‑94 (MQ=60)
                                                                          GAAGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGA                    <  SRR4302540.971571/94‑1 (MQ=60)
                                                                            AGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACC                  <  SRR4302540.2004189/94‑1 (MQ=60)
                                                                            AGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACC                  >  SRR4302540.288577/1‑94 (MQ=60)
                                                                            AGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACC                  >  SRR4302540.7745955/1‑94 (MQ=60)
                                                                            AGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACC                  <  SRR4302540.3981990/94‑1 (MQ=60)
                                                                            AGATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACC                  <  SRR4302540.8262212/94‑1 (MQ=60)
                                                                             GATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCT                 <  SRR4302540.352006/94‑1 (MQ=60)
                                                                             GATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCT                 >  SRR4302540.1219456/1‑94 (MQ=60)
                                                                              ATATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTT                <  SRR4302540.2459303/94‑1 (MQ=60)
                                                                               TATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTC               >  SRR4302540.7467789/1‑94 (MQ=60)
                                                                               TATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTC               >  SRR4302540.1621629/1‑94 (MQ=60)
                                                                               TATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTC               <  SRR4302540.6853018/94‑1 (MQ=60)
                                                                                ATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCA              >  SRR4302540.6914582/1‑94 (MQ=60)
                                                                                ATGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCA              <  SRR4302540.5175661/94‑1 (MQ=60)
                                                                                 TGACCGAGCCGTGCGCAGCTCGCCATGCGGAGTGCCATGCACGCTCTCTTGACATTCAGATTGAGATAAAAGGTCAGGAAGGCATGACCTTCAG             <  SRR4302540.9098210/94‑1 (MQ=60)
                                                                                 TGACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAG             <  SRR4302540.4654193/94‑1 (MQ=60)
                                                                                  GGCCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGC            <  SRR4302540.7525/94‑1 (MQ=60)
                                                                                  GACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGC            >  SRR4302540.502645/1‑94 (MQ=60)
                                                                                  GACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGC            >  SRR4302540.5935789/1‑94 (MQ=60)
                                                                                   ACCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCA           >  SRR4302540.1466111/1‑94 (MQ=60)
                                                                                    CCGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCAC          <  SRR4302540.3810009/94‑1 (MQ=60)
                                                                                     CGAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACG         >  SRR4302540.6250346/1‑94 (MQ=60)
                                                                                      GAGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGC        >  SRR4302540.4542664/1‑94 (MQ=60)
                                                                                       AGCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCA       <  SRR4302540.8559362/94‑1 (MQ=60)
                                                                                        GCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAA      >  SRR4302540.5472370/1‑94 (MQ=60)
                                                                                        GCCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAA      <  SRR4302540.3937865/94‑1 (MQ=60)
                                                                                         CCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAAC     >  SRR4302540.2231519/1‑94 (MQ=60)
                                                                                         CCGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAAC     >  SRR4302540.7649802/1‑94 (MQ=60)
                                                                                          CGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACC    >  SRR4302540.6096711/1‑94 (MQ=60)
                                                                                          CGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACC    <  SRR4302540.10155541/94‑1 (MQ=60)
                                                                                          CGTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACC    >  SRR4302540.1668374/1‑94 (MQ=60)
                                                                                           GTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCT   <  SRR4302540.5079151/94‑1 (MQ=60)
                                                                                           GTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCT   >  SRR4302540.9502473/1‑94 (MQ=60)
                                                                                           GTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCT   <  SRR4302540.9524245/94‑1 (MQ=60)
                                                                                           GTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCT   <  SRR4302540.1450629/94‑1 (MQ=60)
                                                                                           GTGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCT   >  SRR4302540.7287473/1‑94 (MQ=60)
                                                                                            TGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTG  <  SRR4302540.4173775/94‑1 (MQ=60)
                                                                                            TGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTG  <  SRR4302540.6144962/94‑1 (MQ=60)
                                                                                            TGCGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTG  <  SRR4302540.6144963/94‑1 (MQ=60)
                                                                                             |                                                                                            
AAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTACGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTG  >  NC_000913/4472509‑4472694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑