Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 245 579.6 28867798 97.7% 28203838 96.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,998,290 +G coding (379/486 nt) b4566 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,998,2891.G99.8% 1918.4 / NA 488L127P (CTG→CCG) b4566hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  major base G (246/241);  minor base T (0/1);  total (246/242)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 545 total aligned reads displayed.

CCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑GACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCA  >  NC_000913/3998194‑3998384
                                                                                                |                                                                                               
 cTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑g                                                                                                >  2:3423853/1‑96 (MQ=255)
 cTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑g                                                                                                >  2:10391508/1‑96 (MQ=255)
 cTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑g                                                                                                >  1:9790015/1‑96 (MQ=255)
  tGACTGCACCGCAATCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑gt                                                                                               <  1:11321190/96‑2 (MQ=255)
  tGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑gg                                                                                               <  2:5467901/96‑2 (MQ=255)
    aCTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑ggac                                                                                             >  2:6452235/1‑93 (MQ=255)
    aCTGCACCGCAAACTGAATCCCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACCCGCCGCCA‑ggac                                                                                             <  2:1902236/96‑4 (MQ=255)
        cACCGCAAACTGAGTCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGAt                                                                                          >  2:7431181/1‑96 (MQ=255)
        cACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGAt                                                                                          <  1:8531642/96‑1 (MQ=255)
        cACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGAt                                                                                          <  2:46563/96‑1 (MQ=255)
          ccGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATaa                                                                                        >  1:4747873/1‑96 (MQ=255)
           cGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATaaa                                                                                       >  1:9057286/1‑96 (MQ=255)
           cGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATaaa                                                                                       <  2:13741401/96‑1 (MQ=255)
             cAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGa                                                                                     <  1:4973376/96‑1 (MQ=255)
             cAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGa                                                                                     <  2:10685327/96‑1 (MQ=255)
                aCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCTGCGACATAAAACACGCCGCCAGGACGGATAAAGAAAt                                                                                  >  2:12215138/1‑96 (MQ=255)
                aCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAAt                                                                                  <  2:11350468/96‑1 (MQ=255)
                   gAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTcc                                                                               <  1:30906/96‑1 (MQ=255)
                    aaTCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAAAAATTccc                                                                              >  1:6419283/1‑96 (MQ=255)
                    aaTCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACACAAAACACGCCGCCAGGACGGATGAAGAAATTgcc                                                                              >  1:13978729/1‑96 (MQ=255)
                         ctTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCAc                                                                         <  2:1530636/96‑1 (MQ=255)
                          tttACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCAcc                                                                        <  1:6117960/96‑1 (MQ=255)
                          tttACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTCCAcc                                                                        >  1:5761056/1‑96 (MQ=255)
                           ttACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCt                                                                       <  2:277696/96‑1 (MQ=255)
                            tACTGGATTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTg                                                                      >  1:4054874/1‑96 (MQ=255)
                             aCTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGa                                                                     <  1:1435712/96‑1 (MQ=255)
                               tGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAg                                                                   <  2:6132700/96‑1 (MQ=255)
                                ggTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGc                                                                  <  2:8005302/96‑1 (MQ=255)
                                  ttttGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGcta                                                                <  1:11747646/96‑3 (MQ=255)
                                   tttGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCgag                                                               <  1:12357374/96‑1 (MQ=255)
                                   tttGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCgag                                                               <  1:2946382/96‑1 (MQ=255)
                                   gttGAAGGCATCCAGACGACCGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCgag                                                               <  1:3418429/95‑1 (MQ=255)
                                      gAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGAtt                                                            >  2:9143057/1‑96 (MQ=255)
                                       aaGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGAttt                                                           >  2:4158819/1‑96 (MQ=255)
                                       aaGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGAttt                                                           <  2:12811307/96‑1 (MQ=255)
                                       aaGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGAttt                                                           >  2:9449366/1‑96 (MQ=255)
                                        aGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTa                                                          <  2:14237509/96‑1 (MQ=255)
                                         ggCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTaa                                                         >  2:10939667/1‑96 (MQ=255)
                                           cATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACg                                                       >  1:7271275/1‑96 (MQ=255)
                                           cATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACg                                                       >  1:9383171/1‑96 (MQ=255)
                                            aTCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGc                                                      <  1:43538/96‑1 (MQ=255)
                                            aTCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGc                                                      >  1:13738577/1‑96 (MQ=255)
                                             tCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGcc                                                     >  2:7236931/1‑96 (MQ=255)
                                              ccAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCa                                                    <  2:8409030/96‑1 (MQ=255)
                                                aGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCAcc                                                  >  1:1357418/1‑96 (MQ=255)
                                                 gACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCt                                                 <  1:1577017/96‑1 (MQ=255)
                                                    gaacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTct                                              >  2:3645432/1‑96 (MQ=255)
                                                    gaacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTct                                              >  2:5722856/1‑96 (MQ=255)
                                                    gaacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTct                                              <  2:2445726/96‑1 (MQ=255)
                                                    gaacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAAGTCCCTCCACCTGAAGCGAGATTTAACGACACCTTct                                              <  2:12983005/96‑1 (MQ=255)
                                                    gaacgaTGCCGACTTTATCTCCCGCGACAGAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCctct                                              >  1:12066681/1‑96 (MQ=255)
                                                     gaggaTGCCGTCTTTATCTACCGCGACATAAACCACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTctg                                             <  1:14138972/93‑1 (MQ=255)
                                                      acgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTctgc                                            <  2:7836591/96‑1 (MQ=255)
                                                      acgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTctgc                                            >  1:7817544/1‑96 (MQ=255)
                                                       cgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTctgct                                           >  2:11845539/1‑96 (MQ=255)
                                                       cgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTctgct                                           >  1:1906103/1‑96 (MQ=255)
                                                       cgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTctgct                                           >  1:2114879/1‑96 (MQ=255)
                                                        gaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTctgctg                                          >  1:760871/1‑96 (MQ=255)
                                                          tGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGAc                                        >  2:12441525/1‑96 (MQ=255)
                                                          tGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGAc                                        >  1:3669022/1‑96 (MQ=255)
                                                           gCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGAcc                                       <  1:2782700/96‑1 (MQ=255)
                                                           gCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGAcc                                       <  2:216942/96‑1 (MQ=255)
                                                           gCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGAcc                                       >  1:12984705/1‑96 (MQ=255)
                                                            ccGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCg                                      <  2:8918891/96‑1 (MQ=255)
                                                            ccGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCg                                      >  1:11622599/1‑96 (MQ=255)
                                                            ccGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCg                                      <  2:14089047/96‑1 (MQ=255)
                                                             cGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGt                                     <  1:9482817/96‑1 (MQ=255)
                                                             cGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGt                                     >  2:13274069/1‑96 (MQ=255)
                                                             cGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGt                                     <  1:12789750/96‑1 (MQ=255)
                                                               aCTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGttt                                   <  2:4462245/96‑1 (MQ=255)
                                                                cTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGtttt                                  <  2:1788329/96‑1 (MQ=255)
                                                                cTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGtttt                                  <  2:13536578/96‑1 (MQ=255)
                                                                 tttATCTCCCGCGACATAAGACACGCCGACAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCTCCTTCTGCTGACCGTTTTc                                 >  1:83564/1‑96 (MQ=255)
                                                                  ttATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCg                                >  2:5926350/1‑96 (MQ=255)
                                                                    aTCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGAt                              <  2:11025260/96‑1 (MQ=255)
                                                                     tctcCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATg                             >  2:2995234/1‑96 (MQ=255)
                                                                        cccGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTAc                          >  1:3890752/1‑96 (MQ=255)
                                                                        cccGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTAc                          <  1:6782595/96‑1 (MQ=255)
                                                                         ccGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACa                         <  1:9986507/96‑1 (MQ=255)
                                                                           gcgACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACaaa                       <  2:11477374/96‑1 (MQ=255)
                                                                            cgACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAAc                      >  2:9719909/1‑96 (MQ=255)
                                                                             gACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAAcc                     >  1:7990829/1‑96 (MQ=255)
                                                                             gACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAAcc                     <  1:7397495/96‑1 (MQ=255)
                                                                               cATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCCCCTTCTGCTGACCGTTTTCGATGTACAAACCGa                   <  2:385577/96‑1 (MQ=255)
                                                                               cATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGa                   <  1:8683066/96‑1 (MQ=255)
                                                                                aTAAACCACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAg                  <  1:5174475/96‑1 (MQ=255)
                                                                                aTAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAg                  >  2:108244/1‑96 (MQ=255)
                                                                                 tAAAACACGCCGCCAGGACGGATCAAGAAATTCCCTTCACCTGAAGCGAGATTTAACCCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAgc                 <  2:12613817/96‑1 (MQ=255)
                                                                                 tAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAgc                 >  1:13362755/1‑96 (MQ=255)
                                                                                 tAAAACACGCCGCCAGGACGGATAAAGAAAGTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGacc                 <  1:10283295/96‑3 (MQ=255)
                                                                                   aaaCACGCCGCCAGGACGGATAAAGAAATTCCATTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAgcgg               <  1:5419966/96‑1 (MQ=255)
                                                                                    aaCACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAgcggc              <  1:7169673/96‑1 (MQ=255)
                                                                                      ctcgccgcCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGgcgc            >  1:11046944/3‑96 (MQ=255)
                                                                                       acGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGTGCa           >  1:2363537/1‑96 (MQ=255)
                                                                                       acGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCa           >  1:10017942/1‑96 (MQ=255)
                                                                                       acGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCa           <  2:12087058/96‑1 (MQ=255)
                                                                                       acGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCa           >  2:13953317/1‑96 (MQ=255)
                                                                                        cgccgcCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGgcgcct          <  1:10730084/96‑3 (MQ=255)
                                                                                           cgccAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGc       >  2:8645421/1‑96 (MQ=255)
                                                                                               a‑GACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTtca  <  2:5256210/96‑1 (MQ=255)
                                                                                                |                                                                                               
CCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑GACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCA  >  NC_000913/3998194‑3998384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 322 total aligned reads displayed.
TGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑GACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAG  >  NC_000913/3998190‑3998390
                                                                                                     |                                                                                                    
TGGCCCTGACTGCAGCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGACGCCGTCTTTATCTCCCGAGACATAAAACACGCCGCCA‑G                                                                                                      <  SRR4302518.260097/101‑1 (MQ=60)
TGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCNN‑N                                                                                                      >  SRR4302518.10829644/1‑101 (MQ=60)
TGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑G                                                                                                      >  SRR4302518.6231171/1‑101 (MQ=60)
NNNCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑G                                                                                                      <  SRR4302518.8024063/101‑1 (MQ=60)
NNNCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑G                                                                                                      <  SRR4302518.14323533/101‑1 (MQ=60)
 NNNCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑GG                                                                                                     <  SRR4302518.8520980/101‑1 (MQ=60)
 GGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑GG                                                                                                     <  SRR4302518.5135282/101‑1 (MQ=60)
 GGCCCTGACTGCACCGCAAACTGAATCTCGTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCN‑NN                                                                                                     >  SRR4302518.12630425/1‑101 (MQ=60)
 AGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCN‑NN                                                                                                     >  SRR4302518.9245929/1‑101 (MQ=60)
   NNNTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑Ggac                                                                                                   <  SRR4302518.8770299/101‑4 (MQ=60)
   CCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑Ggac                                                                                                   >  SRR4302518.643121/1‑98 (MQ=60)
   CCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑Ggac                                                                                                   >  SRR4302518.1888872/1‑98 (MQ=60)
    CCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACG                                                                                                   <  SRR4302518.4345895/101‑1 (MQ=60)
    CCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACG                                                                                                   >  SRR4302518.225700/1‑101 (MQ=60)
    CCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACG                                                                                                   >  SRR4302518.2931880/1‑101 (MQ=60)
    CCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACG                                                                                                   >  SRR4302518.6509804/1‑101 (MQ=60)
     CTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑Ggannn                                                                                                 >  SRR4302518.11373408/1‑96 (MQ=60)
     CTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑Ggannn                                                                                                 >  SRR4302518.10259330/1‑96 (MQ=60)
     CTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑Ggannn                                                                                                 >  SRR4302518.7407898/1‑96 (MQ=60)
     CTGACTGCACCGCAAACTGAATATCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGACGACTTTATCTCACGCGACATAAAAAACGCCGCCAGGACGG                                                                                                  >  SRR4302518.554344/1‑101 (MQ=60)
      TAACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑Ggacnnn                                                                                                >  SRR4302518.11622653/1‑95 (MQ=60)
       NNNTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGAT                                                                                                <  SRR4302518.8947877/101‑1 (MQ=60)
       NNNTGCACCGCAAACTGAATCTCTTTACTGGTTTGGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGAT                                                                                                <  SRR4302518.9216608/101‑1 (MQ=60)
       GACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCTGCCA‑Gcacgnnn                                                                                               >  SRR4302518.14964505/1‑94 (MQ=60)
        ACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATA                                                                                               >  SRR4302518.4575333/1‑101 (MQ=60)
         nnnccCCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAA                                                                                              <  SRR4302518.10091594/96‑1 (MQ=60)
          TGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAGCACGCCGCCAGGACGGATNNN                                                                                             >  SRR4302518.13388180/1‑101 (MQ=60)
          TGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAA                                                                                             >  SRR4302518.2340264/1‑101 (MQ=60)
          TGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAA                                                                                             <  SRR4302518.5311788/101‑1 (MQ=60)
          NNNACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGACAGGACGGATAAA                                                                                             <  SRR4302518.14045334/101‑1 (MQ=60)
           GCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAG                                                                                            <  SRR4302518.2018549/101‑1 (MQ=60)
             NNNGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAA                                                                                          <  SRR4302518.12474716/101‑1 (MQ=60)
             ACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGCA                                                                                          >  SRR4302518.7326222/1‑101 (MQ=60)
             ACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCAGCGACATAAAACACGCCGCCA‑Ggacggctacannn                                                                                         >  SRR4302518.10146950/1‑88 (MQ=60)
              NNNCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAA                                                                                         <  SRR4302518.7734444/101‑1 (MQ=60)
              CCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAA                                                                                         >  SRR4302518.4996858/1‑101 (MQ=60)
               CGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGANNN                                                                                        >  SRR4302518.9494897/1‑101 (MQ=60)
               CGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGANNN                                                                                        >  SRR4302518.12792359/1‑101 (MQ=60)
                NNNAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATT                                                                                       <  SRR4302518.7674434/101‑1 (MQ=60)
                GCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAANNN                                                                                       >  SRR4302518.8399162/1‑101 (MQ=60)
                  AAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCC                                                                                     <  SRR4302518.3535463/101‑1 (MQ=60)
                  AAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCC                                                                                     >  SRR4302518.5573567/1‑101 (MQ=60)
                  AAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCC                                                                                     <  SRR4302518.32121/101‑1 (MQ=60)
                   NNNTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTACCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCC                                                                                    <  SRR4302518.12689404/101‑1 (MQ=60)
                   AACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCC                                                                                    >  SRR4302518.1030395/1‑101 (MQ=60)
                      TGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTC                                                                                 <  SRR4302518.3577481/101‑1 (MQ=60)
                      NNNATCTCTTTACTGGTTTTGAAGGCATACAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTC                                                                                 <  SRR4302518.10642630/101‑1 (MQ=60)
                       GAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCC                                                                                >  SRR4302518.3903856/1‑101 (MQ=60)
                        AATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACACAAAACACGCCGCCAGGACGGATGAAGAAATTGCCTTNNN                                                                               >  SRR4302518.14618422/1‑101 (MQ=60)
                         NNNTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACC                                                                              <  SRR4302518.9668407/101‑1 (MQ=60)
                         NNNTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACC                                                                              <  SRR4302518.7899309/101‑1 (MQ=60)
                         ATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACC                                                                              <  SRR4302518.2978924/101‑1 (MQ=60)
                          GCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCANNN                                                                             >  SRR4302518.11973640/1‑101 (MQ=60)
                           NNNTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTG                                                                            <  SRR4302518.14483944/101‑1 (MQ=60)
                           CTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTA                                                                            >  SRR4302518.777744/1‑101 (MQ=60)
                           CTCTTTACTGGTTTTGAAGGCATCCAGACGAAAGATGCCGACTTTATCTCCCGCGACATAAAACAGGCCGCCAGGACGGATAAAGAAATTCCGTTCACCTA                                                                            >  SRR4302518.6820791/1‑101 (MQ=60)
                            TCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGA                                                                           >  SRR4302518.312188/1‑101 (MQ=60)
                            TCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGA                                                                           <  SRR4302518.1495937/101‑1 (MQ=60)
                             NNNTACTGGTTTTGAAGGCCTCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGGA                                                                          <  SRR4302518.12008977/101‑1 (MQ=60)
                             CTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAA                                                                          <  SRR4302518.1030714/101‑1 (MQ=60)
                             CTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAA                                                                          <  SRR4302518.4992091/101‑1 (MQ=60)
                             CTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAA                                                                          <  SRR4302518.1945821/101‑1 (MQ=60)
                              TTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGNNN                                                                         >  SRR4302518.11171478/1‑101 (MQ=60)
                              TTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTCCACCTGAAC                                                                         >  SRR4302518.6052992/1‑101 (MQ=60)
                               TTGCTGGTTTGGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACGCGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGC                                                                        <  SRR4302518.2202275/101‑1 (MQ=60)
                               TTACTGGTTTGGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGC                                                                        <  SRR4302518.370943/101‑1 (MQ=60)
                                 NNNGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCTA                                                                      <  SRR4302518.12303499/101‑1 (MQ=60)
                                  CTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCGAGGACGGATAAAGAATTTCACTTCACCTGAAGCGAT                                                                     >  SRR4302518.3136794/1‑101 (MQ=60)
                                  CTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAG                                                                     <  SRR4302518.3084439/101‑1 (MQ=60)
                                     NNNTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATT                                                                  <  SRR4302518.15003028/101‑1 (MQ=60)
                                     NNNTTGAAGGCATCCAGACGAACGATGCAGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATT                                                                  <  SRR4302518.10879446/101‑1 (MQ=60)
                                     GTTTTGAAGGCATCCAGACGAACGATGCCGACTTTACCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATT                                                                  <  SRR4302518.183267/101‑1 (MQ=60)
                                      gatctAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTT                                                                 <  SRR4302518.1381123/96‑1 (MQ=60)
                                      TTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAGACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGANNN                                                                 >  SRR4302518.11455825/1‑101 (MQ=60)
                                      TTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGANNN                                                                 >  SRR4302518.14734228/1‑101 (MQ=60)
                                      TTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCATTCACCTGAAGCGAGANNN                                                                 >  SRR4302518.14386404/1‑101 (MQ=60)
                                        NNNAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAA                                                               <  SRR4302518.10067447/101‑1 (MQ=60)
                                         TGAAGGCATCCAGACGACCGATGCCGACTTTATCCCCCGCGACATAAACCACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAAC                                                              <  SRR4302518.4510020/101‑1 (MQ=60)
                                         TGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAAC                                                              <  SRR4302518.6313386/101‑1 (MQ=60)
                                         TGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAAC                                                              >  SRR4302518.2079306/1‑101 (MQ=60)
                                           AAGGCATCCAGACTAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAANNN                                                            >  SRR4302518.11479465/1‑101 (MQ=60)
                                           AAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAANNN                                                            >  SRR4302518.8830298/1‑101 (MQ=60)
                                            AGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGTCATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACNNN                                                           >  SRR4302518.8471897/1‑101 (MQ=60)
                                             NNNATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCT                                                          <  SRR4302518.11942316/101‑1 (MQ=60)
                                             GGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGNNN                                                          >  SRR4302518.9379564/1‑101 (MQ=60)
                                             GGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCA                                                          <  SRR4302518.6268642/101‑1 (MQ=60)
                                              GCCTCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCAC                                                         <  SRR4302518.5517757/101‑1 (MQ=60)
                                               CATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCNNN                                                        >  SRR4302518.9836918/1‑101 (MQ=60)
                                               CATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCNNN                                                        >  SRR4302518.7650781/1‑101 (MQ=60)
                                               CATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCNNN                                                        >  SRR4302518.9884459/1‑101 (MQ=60)
                                               CATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACC                                                        >  SRR4302518.4169838/1‑101 (MQ=60)
                                               CATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACC                                                        >  SRR4302518.4212725/1‑101 (MQ=60)
                                                NNNCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCCCCT                                                       <  SRR4302518.7555643/101‑1 (MQ=60)
                                                ATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCANNN                                                       >  SRR4302518.8614045/1‑101 (MQ=60)
                                                ATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCANNN                                                       >  SRR4302518.10657851/1‑101 (MQ=60)
                                                ATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCT                                                       <  SRR4302518.1645530/101‑1 (MQ=60)
                                                ATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCT                                                       >  SRR4302518.3408343/1‑101 (MQ=60)
                                                ATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACACCANNN                                                       >  SRR4302518.14367550/1‑101 (MQ=60)
                                                 TCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTT                                                      >  SRR4302518.565076/1‑101 (MQ=60)
                                                  CCAGACGAGCGATGCTGACTTTATCTCCCGCGACATAAAACACGCAGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTC                                                     <  SRR4302518.2688655/101‑1 (MQ=60)
                                                  CCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATGTAACGCCACCTGC                                                     >  SRR4302518.2140417/1‑101 (MQ=60)
                                                   cacacgtacgctggcgttcttcaacCCCGCGACATAAAACACACCGCCAGGAAGGATAAAGAAATTCCCTTCCCCTGAAGCGAGGTTTAAAGCCACCTTCT                                                    <  SRR4302518.663195/76‑1 (MQ=48)
                                                    nnncgcACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAAATCCCTTCACGTGAAGCGAGATTTAACGCCACCTTCTG                                                   <  SRR4302518.11095042/95‑1 (MQ=60)
                                                    nnncagagGATGCCGTCTTTATCTACCGCGACATAAACCACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTG                                                   <  SRR4302518.14787262/93‑1 (MQ=60)
                                                    NNNCGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCCG                                                   <  SRR4302518.13684346/101‑1 (MQ=60)
                                                    AGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTNNN                                                   >  SRR4302518.13530027/1‑101 (MQ=60)
                                                    AGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTG                                                   >  SRR4302518.5027856/1‑101 (MQ=60)
                                                    AGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTG                                                   >  SRR4302518.1413713/1‑101 (MQ=60)
                                                    AGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTG                                                   >  SRR4302518.670622/1‑101 (MQ=60)
                                                    AGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTG                                                   >  SRR4302518.4077058/1‑101 (MQ=60)
                                                     GGCGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCNNN                                                  >  SRR4302518.11598047/1‑101 (MQ=60)
                                                     CACGACCGATCCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTAACCTGAAGCGAGATTTAACGCCACCTTCTGC                                                  <  SRR4302518.3036748/101‑1 (MQ=60)
                                                        GAACTATGCCGACTTTACCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCCCCTGAAGCGAGATTTAACGCCACCTCCTGCTGA                                               <  SRR4302518.2582456/101‑1 (MQ=60)
                                                          ACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGNNN                                             >  SRR4302518.8211607/1‑101 (MQ=60)
                                                          ACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACC                                             <  SRR4302518.82023/101‑1 (MQ=60)
                                                           CGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGANNN                                            >  SRR4302518.15043557/1‑101 (MQ=60)
                                                           CGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCATACCG                                            >  SRR4302518.1427213/1‑101 (MQ=60)
                                                            NNNGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGT                                           <  SRR4302518.13385443/101‑1 (MQ=60)
                                                            GATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCCCCTTCTGCTGACCGT                                           >  SRR4302518.6995224/1‑101 (MQ=60)
                                                            GATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACNNN                                           >  SRR4302518.9925199/1‑101 (MQ=60)
                                                            GATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGT                                           >  SRR4302518.6328313/1‑101 (MQ=60)
                                                              TGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTT                                         >  SRR4302518.3841102/1‑101 (MQ=60)
                                                              TGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTT                                         >  SRR4302518.2337128/1‑101 (MQ=60)
                                                              TGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTT                                         <  SRR4302518.4849771/101‑1 (MQ=60)
                                                              TGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGNNN                                         >  SRR4302518.12560303/1‑101 (MQ=60)
                                                               NNNGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTC                                        <  SRR4302518.14471828/101‑1 (MQ=60)
                                                               GCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTNNN                                        >  SRR4302518.13586355/1‑101 (MQ=60)
                                                                NNNACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTC                                       <  SRR4302518.14458517/101‑1 (MQ=60)
                                                                CCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTNNN                                       >  SRR4302518.8045681/1‑101 (MQ=60)
                                                                CCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTNNN                                       >  SRR4302518.12169919/1‑101 (MQ=60)
                                                                 CTACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTNNN                                      >  SRR4302518.12350908/1‑101 (MQ=60)
                                                                 CGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCG                                      >  SRR4302518.2279296/1‑101 (MQ=60)
                                                                 CGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTNNN                                      >  SRR4302518.10186203/1‑101 (MQ=60)
                                                                  GCCTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGA                                     >  SRR4302518.5306538/1‑101 (MQ=60)
                                                                  GACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTNNN                                     >  SRR4302518.13396962/1‑101 (MQ=60)
                                                                  GACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGA                                     >  SRR4302518.2847072/1‑101 (MQ=60)
                                                                   NNNTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGAT                                    <  SRR4302518.11788726/101‑1 (MQ=60)
                                                                    NNNTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATG                                   <  SRR4302518.14820441/101‑1 (MQ=60)
                                                                    NNNTATCTCCCGCGACATAAAACACGCCACCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATG                                   <  SRR4302518.13132326/101‑1 (MQ=60)
                                                                    CTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGNNN                                   >  SRR4302518.9004492/1‑101 (MQ=60)
                                                                    CTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATG                                   >  SRR4302518.3749358/1‑101 (MQ=60)
                                                                     TTTATCTCCCGCGACATAAGACACGCCGACAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCTCCTTCTGCTGACCGTTTTCGATGT                                  >  SRR4302518.86932/1‑101 (MQ=60)
                                                                      TTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTT                                 <  SRR4302518.7175916/101‑1 (MQ=60)
                                                                      TTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATCTAACGCCACCTTCTGCTGACCGTTTTCGATNNN                                 >  SRR4302518.10387530/1‑101 (MQ=60)
                                                                       TATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCCCCTTCTGCTGACCGTTTTCGATGTAC                                <  SRR4302518.4819458/101‑1 (MQ=60)
                                                                       TATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTAC                                <  SRR4302518.7138604/101‑1 (MQ=60)
                                                                        NNNTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACA                               <  SRR4302518.10461687/101‑1 (MQ=60)
                                                                        ATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACA                               <  SRR4302518.5219515/101‑1 (MQ=60)
                                                                        ATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACA                               <  SRR4302518.3583338/101‑1 (MQ=60)
                                                                         TCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTANNN                              >  SRR4302518.9088641/1‑101 (MQ=60)
                                                                         TCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAA                              >  SRR4302518.3234196/1‑101 (MQ=60)
                                                                         TCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCAGCTGACTGTTTTCGATGTACAA                              >  SRR4302518.4466415/1‑101 (MQ=60)
                                                                          NNNCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAA                             <  SRR4302518.7615447/101‑1 (MQ=60)
                                                                           TCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACTGTTTTCGATGTACAAAC                            >  SRR4302518.3943807/1‑101 (MQ=60)
                                                                           TCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACANNN                            >  SRR4302518.10526928/1‑101 (MQ=60)
                                                                            NNNGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACC                           <  SRR4302518.7780287/101‑1 (MQ=60)
                                                                            CCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACC                           >  SRR4302518.4075681/1‑101 (MQ=60)
                                                                              NNNGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGA                         <  SRR4302518.9105374/101‑1 (MQ=60)
                                                                              CGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGA                         >  SRR4302518.6649494/1‑101 (MQ=60)
                                                                               GCGACATAAAACACGCCGCCAGGGCGGGTAAAGAAAATCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTGTGGTTGTACAAACCAAG                        >  SRR4302518.5872202/1‑101 (MQ=60)
                                                                               GCGACATAAAACACGCCGCCAGGACTGATAAAGAAATTCCCTTCACCTGAAGCGAGAGTTAACGCCACCTTCTGCTGACCGTTTTCGATGTCCAAACCGAG                        <  SRR4302518.1992807/101‑1 (MQ=60)
                                                                                nnncatacacccCGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGACC                       <  SRR4302518.14167443/89‑1 (MQ=60)
                                                                                 NNNATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCG                      <  SRR4302518.14255793/101‑1 (MQ=60)
                                                                                 GCCATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGANNN                      >  SRR4302518.7945327/1‑101 (MQ=60)
                                                                                 GACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGANNN                      >  SRR4302518.8389608/1‑101 (MQ=60)
                                                                                 GACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCG                      >  SRR4302518.5411061/1‑101 (MQ=60)
                                                                                  ACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGNNN                     >  SRR4302518.9527458/1‑101 (MQ=60)
                                                                                  ACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGNNN                     >  SRR4302518.13007067/1‑101 (MQ=60)
                                                                                  ACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCATTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGG                     <  SRR4302518.5696718/101‑1 (MQ=60)
                                                                                   NNNAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGC                    <  SRR4302518.7546234/101‑1 (MQ=60)
                                                                                    AGAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGNNN                   >  SRR4302518.9074937/1‑101 (MQ=60)
                                                                                     TAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGNNN                  >  SRR4302518.13977550/1‑101 (MQ=60)
                                                                                     TAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGNNN                  >  SRR4302518.7849024/1‑101 (MQ=60)
                                                                                      NNNACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGGA                 <  SRR4302518.12213946/101‑1 (MQ=60)
                                                                                      AAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCNNN                 >  SRR4302518.8273156/1‑101 (MQ=60)
                                                                                      AAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCNNN                 >  SRR4302518.9540671/1‑101 (MQ=60)
                                                                                       NNNCACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCCT                <  SRR4302518.11234203/101‑1 (MQ=60)
                                                                                       NNNCACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCAT                <  SRR4302518.14102681/101‑1 (MQ=60)
                                                                                       AAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCAT                <  SRR4302518.1319980/101‑1 (MQ=60)
                                                                                         NNNCGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACTGTTTTCGATGTACAAACCGAGCGGCGCATAG              <  SRR4302518.12926530/101‑1 (MQ=60)
                                                                                         NNNCGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAG              <  SRR4302518.15054731/101‑1 (MQ=60)
                                                                                          CTCGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATNNN             >  SRR4302518.11563973/1‑101 (MQ=60)
                                                                                          CACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCAGAGC             >  SRR4302518.1461747/1‑101 (MQ=60)
                                                                                           nnnccgtcagGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCT            <  SRR4302518.12250339/91‑1 (MQ=60)
                                                                                           NNNCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCT            <  SRR4302518.13939945/101‑1 (MQ=60)
                                                                                           ACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGTGCATAGCT            >  SRR4302518.2478320/1‑101 (MQ=60)
                                                                                            CGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTT           <  SRR4302518.6860373/101‑1 (MQ=60)
                                                                                             nnngcccgGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACCAACCGAGCGGCGCATAGCTTT          <  SRR4302518.13467660/93‑1 (MQ=60)
                                                                                             GCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTT          >  SRR4302518.6556126/1‑101 (MQ=60)
                                                                                              nnnccagGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTC         <  SRR4302518.8886011/94‑1 (MQ=60)
                                                                                                gacagGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTNNN       >  SRR4302518.8554733/6‑101 (MQ=60)
                                                                                                GCCAGGACGGATAAAGAAATTCCCTTCCCCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCAT       <  SRR4302518.1541565/101‑1 (MQ=60)
                                                                                                GCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTNNN       >  SRR4302518.9387137/1‑101 (MQ=60)
                                                                                                GCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCAT       >  SRR4302518.1908657/1‑101 (MQ=60)
                                                                                                  cggGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCANNN     >  SRR4302518.12064429/4‑101 (MQ=60)
                                                                                                  cagGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCA     >  SRR4302518.2916188/4‑101 (MQ=60)
                                                                                                  cagGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCANNN     >  SRR4302518.14274028/4‑101 (MQ=60)
                                                                                                   agGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATNNN    >  SRR4302518.11768508/3‑101 (MQ=60)
                                                                                                   agGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCAT    >  SRR4302518.2773196/3‑101 (MQ=60)
                                                                                                   G‑GACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATA   >  SRR4302518.5873410/1‑101 (MQ=60)
                                                                                                     |                                                                                                    
TGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑GACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAG  >  NC_000913/3998190‑3998390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑