Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,998,290 +G coding (379/486 nt) b4566 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,998,2891.G100.0% 795.0 / NA 246L127P (CTG→CCG) b4566hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (129/117);  total (129/117)

Only 100 of 262 total aligned reads displayed.

GACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑GACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCAT  >  NC_000913/3998197‑3998376
                                                                                             |                                                                                       
gACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCa                                                                                          >  1:5489166/1‑93 (MQ=255)
  cTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑gg                                                                                       <  2:2934014/93‑2 (MQ=255)
   tGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑gga                                                                                      <  1:2019439/93‑3 (MQ=255)
    gCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATTCCGACTTTTTCTCTCTCTACATAAAAAACGGCGCCacgac                                                                                      >  2:1328708/1‑89 (MQ=255)
    gCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑ggac                                                                                     >  1:4753928/1‑90 (MQ=255)
    gCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑ggac                                                                                     <  2:5981372/93‑4 (MQ=255)
     cACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑ggacg                                                                                    >  2:6100679/1‑89 (MQ=255)
     cACCGCAAACGGAATCTCTTTACTGGTTTGGAAGCCATCCAGCCGCACGTTGCCGGCTTTATCTCCCGCGACATAAACCACGCCGCCA‑ggacg                                                                                    <  1:4336579/93‑5 (MQ=255)
       ccGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGa                                                                                   <  1:2860792/93‑1 (MQ=255)
       ccGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGa                                                                                   >  1:5925476/1‑93 (MQ=255)
       ccGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGa                                                                                   <  1:2215787/93‑1 (MQ=255)
        cGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGAt                                                                                  <  2:5118812/93‑1 (MQ=255)
         gCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATa                                                                                 >  2:487234/1‑93 (MQ=255)
          cAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATaa                                                                                >  1:39786/1‑93 (MQ=255)
           aaaCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATaaa                                                                               >  1:5535652/1‑93 (MQ=255)
           aaaCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATaaa                                                                               >  2:2382239/1‑93 (MQ=255)
           aaaCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATaaa                                                                               <  1:3641010/93‑1 (MQ=255)
             aCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGa                                                                             >  1:1746378/1‑93 (MQ=255)
             aCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGa                                                                             >  1:5206637/1‑93 (MQ=255)
              cTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGaa                                                                            >  2:2236541/1‑93 (MQ=255)
              cTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGaa                                                                            <  2:3510636/93‑1 (MQ=255)
               tGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGaaa                                                                           <  1:2719230/93‑1 (MQ=255)
                 aaTCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAAtt                                                                         <  2:154436/93‑1 (MQ=255)
                  aTCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTc                                                                        <  2:5734341/93‑1 (MQ=255)
                   tctcTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTcc                                                                       >  2:4910187/1‑93 (MQ=255)
                   tctcTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTcc                                                                       >  2:3588343/1‑93 (MQ=255)
                    ctctTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTccc                                                                      <  2:810054/93‑1 (MQ=255)
                     tctTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCt                                                                     >  2:4172322/1‑93 (MQ=255)
                      ctTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCtt                                                                    <  1:1824728/93‑1 (MQ=255)
                        ttACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCa                                                                  >  1:2337419/1‑93 (MQ=255)
                          aCTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCAcc                                                                >  1:5282651/1‑93 (MQ=255)
                          aCTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCAcc                                                                >  1:5069844/1‑93 (MQ=255)
                             ggTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGa                                                             >  1:1575286/1‑93 (MQ=255)
                             ggTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGa                                                             >  1:2643061/1‑93 (MQ=255)
                              gTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGaa                                                            <  1:6026186/93‑1 (MQ=255)
                              gTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGaa                                                            >  1:3264316/1‑93 (MQ=255)
                              gTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGaa                                                            >  2:5332960/1‑93 (MQ=255)
                                tttGAAGCCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGc                                                          <  2:216626/93‑1 (MQ=255)
                                    aaGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCgaga                                                      <  1:6244152/93‑1 (MQ=255)
                                    aaGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCgaga                                                      <  1:1477117/93‑1 (MQ=255)
                                     aGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGAt                                                     <  1:365609/93‑1 (MQ=255)
                                        cATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTa                                                  <  2:3000020/93‑1 (MQ=255)
                                        cATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTa                                                  >  1:3817236/1‑93 (MQ=255)
                                         aTCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTaa                                                 >  1:1438485/1‑93 (MQ=255)
                                         aTCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTaa                                                 <  2:762955/93‑1 (MQ=255)
                                          tCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAAc                                                <  2:5645660/93‑1 (MQ=255)
                                             aGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGcc                                             >  2:3209008/1‑93 (MQ=255)
                                             aGACAAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGcc                                             <  2:551824/93‑1 (MQ=255)
                                              gACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCa                                            <  2:1190038/93‑1 (MQ=255)
                                              gACGAACGATGCCGACTTTATCTCCCGCGAAATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCa                                            >  2:4454269/1‑93 (MQ=255)
                                               acgaacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCAc                                           <  2:4346423/93‑1 (MQ=255)
                                                cgaacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCAcc                                          <  1:2488506/93‑1 (MQ=255)
                                                cgaacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCAcc                                          >  1:4570376/1‑93 (MQ=255)
                                                 caacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCt                                         <  2:141082/92‑1 (MQ=255)
                                                  aacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCtt                                        >  1:5560006/1‑93 (MQ=255)
                                                   acgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTc                                       >  1:860666/1‑93 (MQ=255)
                                                   acgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTc                                       <  2:5941998/93‑1 (MQ=255)
                                                     gaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTctg                                     >  2:101733/1‑93 (MQ=255)
                                                      aTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTctgc                                    >  2:6278258/1‑93 (MQ=255)
                                                      aTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTctgc                                    >  2:1828833/1‑93 (MQ=255)
                                                       tGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTctgct                                   >  1:1123169/1‑93 (MQ=255)
                                                          cGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGAc                                <  2:1246059/93‑1 (MQ=255)
                                                          cGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGAc                                <  1:1156971/93‑1 (MQ=255)
                                                           gACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGAcc                               >  1:1016507/1‑93 (MQ=255)
                                                            aCTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCg                              >  1:4952995/1‑93 (MQ=255)
                                                            aCTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCg                              >  2:265132/1‑93 (MQ=255)
                                                             cTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGt                             >  1:1242162/1‑93 (MQ=255)
                                                              tttATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGtt                            <  1:5806518/93‑1 (MQ=255)
                                                              tttATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGtt                            >  2:4691994/1‑93 (MQ=255)
                                                              tttATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGAGTTAACGCCACCTTCTGCTGACCggt                            >  2:2445735/1‑91 (MQ=255)
                                                               ttATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGttt                           >  1:683739/1‑93 (MQ=255)
                                                               ttATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGttt                           <  1:1983802/93‑1 (MQ=255)
                                                                tATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGtttt                          >  1:40962/1‑93 (MQ=255)
                                                                 aTCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTc                         <  1:2654255/93‑1 (MQ=255)
                                                                 aTCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTc                         <  2:5712567/93‑1 (MQ=255)
                                                                  tctcCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCg                        >  1:4255926/1‑93 (MQ=255)
                                                                   ctcCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGa                       >  1:6115336/1‑93 (MQ=255)
                                                                   ctcCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGa                       <  2:5602238/93‑1 (MQ=255)
                                                                   ctcCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGa                       <  2:5247623/93‑1 (MQ=255)
                                                                    tcCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTGCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGAt                      <  1:3525860/93‑1 (MQ=255)
                                                                        gcgACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTAc                  >  1:3065339/1‑93 (MQ=255)
                                                                        gcgACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTAc                  >  2:1522418/1‑93 (MQ=255)
                                                                         ctaCATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCGCCTGAAGCGAGATTTAACGCCGCCTTCTGCTGACCGTTTTCGATGTACa                 <  1:4858756/91‑1 (MQ=255)
                                                                         cgACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACa                 <  1:105661/93‑1 (MQ=255)
                                                                         cgACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACa                 <  1:2373381/93‑1 (MQ=255)
                                                                          gACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACaa                <  2:1767513/93‑1 (MQ=255)
                                                                           aCATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACaac               >  2:132123/1‑92 (MQ=255)
                                                                            cATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAAc              <  1:5624336/93‑1 (MQ=255)
                                                                            cATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAAc              <  1:1536302/93‑1 (MQ=255)
                                                                            cATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAAc              >  1:2797505/1‑93 (MQ=255)
                                                                             aTAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAAcc             >  2:3775622/1‑93 (MQ=255)
                                                                             aTAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAAcc             <  1:2099486/93‑1 (MQ=255)
                                                                                aaaCACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAg          <  2:1671368/93‑1 (MQ=255)
                                                                                aaaCACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACAGTTTTCGATGTACAAACCGAg          >  1:4033713/1‑93 (MQ=255)
                                                                                 aaCACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAgc         >  2:2846706/1‑93 (MQ=255)
                                                                                    acGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAgcggc      <  1:5684208/93‑1 (MQ=255)
                                                                                      gccgccAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGgcgc    <  2:3263317/93‑1 (MQ=255)
                                                                                       ccgccAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCa   <  1:4523112/93‑1 (MQ=255)
                                                                                        cgccAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCAt  <  2:2044352/93‑1 (MQ=255)
                                                                                        cgccAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCAt  <  2:4513217/93‑1 (MQ=255)
                                                                                             |                                                                                       
GACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑GACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCAT  >  NC_000913/3998197‑3998376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑GACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTT  >  NC_000913/3998197‑3998382
                                                                                              |                                                                                            
GACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑G                                                                                              <  SRR4302533.2665188/94‑1 (MQ=60)
 ACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑GG                                                                                             >  SRR4302533.5264204/1‑94 (MQ=60)
   TGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑Ggac                                                                                           >  SRR4302533.4782745/1‑91 (MQ=60)
   TGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑Ggac                                                                                           <  SRR4302533.2031876/94‑4 (MQ=60)
     CACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGG                                                                                          <  SRR4302533.2370805/94‑1 (MQ=60)
     CACCGCAAACGGAATCTCTTTACTGGTTTGGAAGCCATCCAGCCGCACGTTGCCGGCTTTATCTCCCGCGACATAAACCACGCCGCCAGGACGG                                                                                          <  SRR4302533.4362712/94‑1 (MQ=53)
      ACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGA                                                                                         <  SRR4302533.5445604/94‑1 (MQ=60)
      ACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGA                                                                                         >  SRR4302533.5961298/1‑94 (MQ=60)
       CCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGAT                                                                                        <  SRR4302533.2878392/94‑1 (MQ=60)
       CCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGAT                                                                                        <  SRR4302533.2229467/94‑1 (MQ=60)
        CGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATA                                                                                       <  SRR4302533.3575058/94‑1 (MQ=60)
         GCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAA                                                                                      <  SRR4302533.1830732/94‑1 (MQ=60)
         GCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAA                                                                                      >  SRR4302533.40050/1‑94 (MQ=60)
          CAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAA                                                                                     >  SRR4302533.5569076/1‑94 (MQ=60)
          CAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAA                                                                                     >  SRR4302533.1267368/1‑94 (MQ=60)
           AAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAG                                                                                    <  SRR4302533.3663401/94‑1 (MQ=60)
            AACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGA                                                                                   >  SRR4302533.5238006/1‑94 (MQ=60)
            AACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGA                                                                                   >  SRR4302533.1757000/1‑94 (MQ=60)
            AACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGA                                                                                   >  SRR4302533.4647906/1‑94 (MQ=60)
            AACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGA                                                                                   >  SRR4302533.2547433/1‑94 (MQ=60)
              CTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAA                                                                                 >  SRR4302533.4057582/1‑94 (MQ=60)
               TGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAAT                                                                                >  SRR4302533.5720546/1‑94 (MQ=60)
               TGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAAT                                                                                <  SRR4302533.331021/94‑1 (MQ=60)
               TGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAAT                                                                                <  SRR4302533.2735918/94‑1 (MQ=60)
               TGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAAT                                                                                <  SRR4302533.2735919/94‑1 (MQ=60)
               TGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAAT                                                                                >  SRR4302533.1158107/1‑94 (MQ=60)
               TGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAAT                                                                                >  SRR4302533.2011184/1‑94 (MQ=60)
                 AATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTC                                                                              >  SRR4302533.300192/1‑94 (MQ=60)
                  ATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCC                                                                             <  SRR4302533.1709050/94‑1 (MQ=60)
                  ATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCC                                                                             >  SRR4302533.1812924/1‑94 (MQ=60)
                  ATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCC                                                                             <  SRR4302533.1998360/94‑1 (MQ=60)
                    CTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCT                                                                           >  SRR4302533.2619969/1‑94 (MQ=60)
                    CTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCT                                                                           <  SRR4302533.2228003/94‑1 (MQ=60)
                    CTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCT                                                                           >  SRR4302533.4100497/1‑94 (MQ=60)
                      CTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTC                                                                         >  SRR4302533.2081880/1‑94 (MQ=60)
                      CTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTC                                                                         <  SRR4302533.1835875/94‑1 (MQ=60)
                      CTTGACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTC                                                                         <  SRR4302533.2724754/94‑1 (MQ=60)
                       TTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCA                                                                        >  SRR4302533.2351886/1‑94 (MQ=60)
                       TTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCA                                                                        >  SRR4302533.5138266/1‑94 (MQ=60)
                        TTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCAC                                                                       >  SRR4302533.1339940/1‑94 (MQ=60)
                        TTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCAC                                                                       >  SRR4302533.2423213/1‑94 (MQ=60)
                         TACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACC                                                                      >  SRR4302533.3585517/1‑94 (MQ=60)
                         TACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACC                                                                      >  SRR4302533.5100456/1‑94 (MQ=60)
                         TACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACC                                                                      >  SRR4302533.5314298/1‑94 (MQ=60)
                         TACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACC                                                                      >  SRR4302533.3756883/1‑94 (MQ=60)
                           CTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTG                                                                    >  SRR4302533.5094193/1‑94 (MQ=60)
                            TGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGA                                                                   >  SRR4302533.1584772/1‑94 (MQ=60)
                            TGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGA                                                                   >  SRR4302533.2659300/1‑94 (MQ=60)
                             GGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAA                                                                  >  SRR4302533.3284453/1‑94 (MQ=60)
                             GGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAA                                                                  <  SRR4302533.2820202/94‑1 (MQ=60)
                             GGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAA                                                                  >  SRR4302533.12421/1‑94 (MQ=60)
                              GTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAG                                                                 <  SRR4302533.2806085/94‑1 (MQ=60)
                              GTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAG                                                                 <  SRR4302533.1610927/94‑1 (MQ=60)
                              GTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAG                                                                 <  SRR4302533.6062704/94‑1 (MQ=60)
                                TTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCACGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCG                                                               >  SRR4302533.5917569/1‑94 (MQ=60)
                                  TGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAG                                                             <  SRR4302533.4346875/94‑1 (MQ=60)
                                  TGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAG                                                             <  SRR4302533.5543840/94‑1 (MQ=60)
                                  TGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAG                                                             <  SRR4302533.6068940/94‑1 (MQ=60)
                                    AAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGAT                                                           <  SRR4302533.1486044/94‑1 (MQ=60)
                                    AAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGAT                                                           <  SRR4302533.6281529/94‑1 (MQ=60)
                                     AGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATT                                                          <  SRR4302533.5321445/94‑1 (MQ=60)
                                     AGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATT                                                          <  SRR4302533.367822/94‑1 (MQ=60)
                                      GGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTT                                                         <  SRR4302533.5453445/94‑1 (MQ=60)
                                       GCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTA                                                        >  SRR4302533.3840577/1‑94 (MQ=60)
                                        CATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAA                                                       >  SRR4302533.1447167/1‑94 (MQ=60)
                                         ATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAAC                                                      <  SRR4302533.6144291/94‑1 (MQ=60)
                                         ATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAAC                                                      <  SRR4302533.1482816/94‑1 (MQ=60)
                                         ATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACCGCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAAC                                                      >  SRR4302533.4109637/1‑94 (MQ=60)
                                          TCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACG                                                     <  SRR4302533.5265385/94‑1 (MQ=60)
                                           CCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGC                                                    >  SRR4302533.3075233/1‑94 (MQ=60)
                                            CAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCC                                                   >  SRR4302533.4921943/1‑94 (MQ=60)
                                            CAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCC                                                   >  SRR4302533.568902/1‑94 (MQ=60)
                                            CAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCC                                                   <  SRR4302533.6315359/94‑1 (MQ=60)
                                            CAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCA                                                   >  SRR4302533.1973558/1‑94 (MQ=60)
                                             AGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCA                                                  >  SRR4302533.4856910/1‑94 (MQ=60)
                                             AGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCA                                                  >  SRR4302533.2107616/1‑94 (MQ=60)
                                              GACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCAC                                                 <  SRR4302533.4278860/94‑1 (MQ=60)
                                               ACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACC                                                >  SRR4302533.4598075/1‑94 (MQ=60)
                                                CGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCT                                               <  SRR4302533.2503832/94‑1 (MQ=60)
                                                 GAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTT                                              >  SRR4302533.5593609/1‑94 (MQ=60)
                                                  AACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTC                                             >  SRR4302533.6041979/1‑94 (MQ=60)
                                                  AACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTC                                             >  SRR4302533.865709/1‑94 (MQ=60)
                                                   ACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCT                                            >  SRR4302533.5668901/1‑94 (MQ=60)
                                                    CGATGCCGACTTTATCTCCCGCGACATAAAACACGCGGCCAGGACGGATAAAAAAATTCACTTCACCTGAAGCGAGATTTAACACCACCTTCTG                                           >  SRR4302533.3931479/1‑94 (MQ=60)
                                                    CGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTG                                           <  SRR4302533.1026241/94‑1 (MQ=60)
                                                    CGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTG                                           <  SRR4302533.3119449/94‑1 (MQ=60)
                                                    CGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTG                                           >  SRR4302533.3931478/1‑94 (MQ=60)
                                                      ATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCT                                         >  SRR4302533.1129834/1‑94 (MQ=60)
                                                      ATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCT                                         >  SRR4302533.5744996/1‑94 (MQ=60)
                                                       TGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTG                                        >  SRR4302533.1102675/1‑94 (MQ=60)
                                                         CCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGAC                                      >  SRR4302533.2286136/1‑94 (MQ=60)
                                                          CGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACC                                     >  SRR4302533.5581222/1‑94 (MQ=60)
                                                          CGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACC                                     >  SRR4302533.1022481/1‑94 (MQ=60)
                                                          CGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACC                                     >  SRR4302533.1540670/1‑94 (MQ=60)
                                                          CGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACC                                     >  SRR4302533.2331110/1‑94 (MQ=60)
                                                          CGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACC                                     <  SRR4302533.1200905/94‑1 (MQ=60)
                                                          CGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACC                                     <  SRR4302533.1163912/94‑1 (MQ=60)
                                                           GACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCGCCTTCTGCTGACCG                                    <  SRR4302533.158370/94‑1 (MQ=60)
                                                           GACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCG                                    >  SRR4302533.688369/1‑94 (MQ=60)
                                                           GACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCG                                    >  SRR4302533.3762112/1‑94 (MQ=60)
                                                           GACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCG                                    >  SRR4302533.4982994/1‑94 (MQ=60)
                                                            ACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGT                                   >  SRR4302533.5576604/1‑94 (MQ=60)
                                                            ACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGT                                   >  SRR4302533.1249582/1‑94 (MQ=60)
                                                            ACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGT                                   <  SRR4302533.4557215/94‑1 (MQ=60)
                                                             CTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTT                                  <  SRR4302533.680062/94‑1 (MQ=60)
                                                              TTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTT                                 <  SRR4302533.5841581/94‑1 (MQ=60)
                                                              TTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTT                                 >  SRR4302533.687783/1‑94 (MQ=60)
                                                              TTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTT                                 >  SRR4302533.5970470/1‑94 (MQ=60)
                                                               TTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTT                                >  SRR4302533.41243/1‑94 (MQ=60)
                                                               TTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTT                                <  SRR4302533.1995941/94‑1 (MQ=60)
                                                                TATCCCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTC                               <  SRR4302533.1520094/94‑1 (MQ=60)
                                                                 ATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCG                              >  SRR4302533.4281585/1‑94 (MQ=60)
                                                                 ATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCG                              <  SRR4302533.2670559/94‑1 (MQ=60)
                                                                  TCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGA                             >  SRR4302533.6152232/1‑94 (MQ=60)
                                                                    TCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTGCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATG                           <  SRR4302533.3547586/94‑1 (MQ=60)
                                                                      CCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTA                         <  SRR4302533.4987636/94‑1 (MQ=60)
                                                                       CGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTAC                        >  SRR4302533.3084168/1‑94 (MQ=60)
                                                                         CTACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCGCCTGAAGCGAGATTTAACGCCGCCTTCTGCTGACCGTTTTCGATGTACAA                      <  SRR4302533.4888207/94‑1 (MQ=60)
                                                                         CGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAA                      <  SRR4302533.2388069/94‑1 (MQ=60)
                                                                         CGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAA                      <  SRR4302533.2461649/94‑1 (MQ=60)
                                                                         CGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAA                      <  SRR4302533.6162324/94‑1 (MQ=60)
                                                                         CGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAA                      <  SRR4302533.106320/94‑1 (MQ=60)
                                                                         CGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAA                      <  SRR4302533.873657/94‑1 (MQ=60)
                                                                          GACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAA                     >  SRR4302533.451758/1‑94 (MQ=60)
                                                                           ACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAAC                    >  SRR4302533.2814710/1‑94 (MQ=60)
                                                                           ACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAAC                    <  SRR4302533.4620180/94‑1 (MQ=60)
                                                                            CATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACC                   <  SRR4302533.5658331/94‑1 (MQ=60)
                                                                            CATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACC                   <  SRR4302533.1545533/94‑1 (MQ=60)
                                                                            CATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACC                   <  SRR4302533.1114753/94‑1 (MQ=60)
                                                                             ATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCG                  <  SRR4302533.2112438/94‑1 (MQ=60)
                                                                             ATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCG                  <  SRR4302533.4544046/94‑1 (MQ=60)
                                                                             ATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCG                  >  SRR4302533.3666356/1‑94 (MQ=60)
                                                                               AAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACAGTTTTCGATGTACAAACCGAG                >  SRR4302533.4058265/1‑94 (MQ=60)
                                                                                AAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGC               <  SRR4302533.4053324/94‑1 (MQ=60)
                                                                                AAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGC               <  SRR4302533.3366262/94‑1 (MQ=60)
                                                                                AAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGC               >  SRR4302533.4299332/1‑94 (MQ=60)
                                                                                 AACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCG              >  SRR4302533.5614403/1‑94 (MQ=60)
                                                                                 AACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCG              >  SRR4302533.4908036/1‑94 (MQ=60)
                                                                                    ACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCG           <  SRR4302533.5718543/94‑1 (MQ=60)
                                                                                       CCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCAT        <  SRR4302533.4550492/94‑1 (MQ=60)
                                                                                        CGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATA       >  SRR4302533.3596063/1‑94 (MQ=60)
                                                                                         GCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAG      <  SRR4302533.1042843/94‑1 (MQ=60)
                                                                                         GCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAG      <  SRR4302533.4334108/94‑1 (MQ=60)
                                                                                          CCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGC     >  SRR4302533.1872188/1‑94 (MQ=60)
                                                                                          CCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGC     >  SRR4302533.215263/1‑94 (MQ=60)
                                                                                           cagGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCT    <  SRR4302533.2134934/91‑1 (MQ=60)
                                                                                           cagGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCT    >  SRR4302533.2176321/4‑94 (MQ=60)
                                                                                           cagGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCT    >  SRR4302533.4109894/4‑94 (MQ=60)
                                                                                            G‑GACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTT  <  SRR4302533.5647732/94‑1 (MQ=60)
                                                                                            G‑GACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTT  >  SRR4302533.6073885/1‑94 (MQ=60)
                                                                                              |                                                                                            
GACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑GACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTT  >  NC_000913/3998197‑3998382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑