Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 245 579.6 28867798 97.7% 28203838 96.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,008,411 +C coding (494/516 nt) b4555 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,008,4101.C98.2% 870.7 / 4.5 227coding (493/516 nt)b4555hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (1/3);  new base C (102/121);  total (103/124)
Fisher's exact test for biased strand distribution p-value = 6.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 229 total aligned reads displayed.

CTGTCTGGAAGTGATGGCGGGCGGCGTTTC‑AAAGGCCATGCGCTGGAAG‑GCTGGCG‑‑‑‑AACT‑G‑GCTACAG‑C‑TGAAGGATTG‑ATTGCGT‑T‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGAAA‑GCTGCATTATGGGCAGTGGCCA  >  NC_000913/4008343‑4008494
                                                                            |                                                                                           
cTGTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGt‑t                                                                       <  2:3761128/96‑1 (MQ=255)
 tGTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGt‑tt                                                                      <  1:6839357/96‑2 (MQ=255)
 tGTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGt‑tt                                                                      <  2:2926582/96‑2 (MQ=255)
 tGTCTGGAAGTGATGGCGGGCGGAGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGt‑tt                                                                      <  1:12583935/96‑2 (MQ=255)
  gTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGt‑ttg                                                                     <  2:5241731/96‑3 (MQ=255)
  gTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGt‑ttg                                                                     <  1:9864495/96‑3 (MQ=255)
  gTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGt‑ttg                                                                     >  2:10400554/1‑94 (MQ=255)
  gTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGt‑ttg                                                                     >  2:9040325/1‑94 (MQ=255)
   tCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGt‑ttgg                                                                    >  1:3449894/1‑93 (MQ=255)
   tCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGt‑ttgg                                                                    >  2:7833193/1‑93 (MQ=255)
    cTGGATGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGt‑ttggt                                                                   <  1:4762551/96‑5 (MQ=255)
    cTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGt‑ttggt                                                                   <  2:7567338/96‑5 (MQ=255)
            gATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGAt                                                           <  1:1072092/96‑1 (MQ=255)
            gATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGAt                                                           <  1:11217018/96‑1 (MQ=255)
            gATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGAt                                                           <  2:1225594/96‑1 (MQ=255)
            gATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGAGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGAt                                                           <  2:1017290/96‑1 (MQ=255)
             aTGGCGGGCGGCGTTTCAAAAGGCCGTGCGCTGGACGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTGTTGCGT‑TTGGTGACGGGATg                                                          <  2:6118481/96‑1 (MQ=255)
             aTGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATg                                                          <  1:13060395/96‑1 (MQ=255)
             aTGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATg                                                          <  2:161048/96‑1 (MQ=255)
             aTGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATg                                                          <  1:11708699/96‑1 (MQ=255)
              tGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGa                                                         >  2:4766450/1‑96 (MQ=255)
               ggcgggcgGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGACGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGTTGaa                                                        >  1:12912062/1‑96 (MQ=255)
               ggcgggcgGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTCCAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGaa                                                        >  2:9543973/1‑96 (MQ=255)
                gcgggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAac                                                       <  1:10467316/96‑1 (MQ=255)
                 cgggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAacg                                                      <  1:4331098/96‑1 (MQ=255)
                 cgggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAacg                                                      <  2:2577626/96‑1 (MQ=255)
                 cgggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAacg                                                      <  1:2013842/96‑1 (MQ=255)
                 cgggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAacg                                                      <  1:13978146/96‑1 (MQ=255)
                 cgggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAacg                                                      >  1:13181770/1‑96 (MQ=255)
                 cgggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGAGT‑TTGGTGACGGGATGAacg                                                      >  2:9604794/1‑96 (MQ=255)
                  gggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAacga                                                     <  1:6963532/96‑1 (MQ=255)
                  gggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAacga                                                     >  2:10446427/1‑96 (MQ=255)
                  gggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAacga                                                     >  2:6681137/1‑96 (MQ=255)
                  gggcggCGTGTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAacga                                                     <  1:380389/96‑1 (MQ=255)
                   ggcggcGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAacgac                                                    >  1:12862918/1‑96 (MQ=255)
                   ggcggcGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAacgac                                                    >  2:11684656/1‑96 (MQ=255)
                   ggcggcGTATCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACT‑GAGCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAacgtc                                                    <  1:7369695/96‑3 (MQ=255)
                    gcggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAacgacg                                                   <  2:12997738/96‑1 (MQ=255)
                     cggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAAcgccgc                                                  <  1:781157/96‑1 (MQ=255)
                     cggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGc                                                  <  2:6956805/96‑1 (MQ=255)
                     cggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGc                                                  >  1:7288324/1‑96 (MQ=255)
                      ggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGAGGcc                                                 >  1:792971/1‑96 (MQ=255)
                      ggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGcc                                                 >  1:7394350/1‑96 (MQ=255)
                      ggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GATACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGcc                                                 <  1:11448052/96‑1 (MQ=255)
                      ggcgTTTCAAAAGGCCATGCGCTGGAAGCGGGGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGcc                                                 >  2:1886487/1‑96 (MQ=255)
                       gcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCg                                                >  2:4457700/1‑96 (MQ=255)
                        cgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGa                                               >  1:4703266/1‑96 (MQ=255)
                        cgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGa                                               <  2:11320077/96‑1 (MQ=255)
                        cgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGa                                               >  1:4288595/1‑96 (MQ=255)
                         gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGaa                                              <  1:10187737/96‑1 (MQ=255)
                         gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGaa                                              >  2:7698105/1‑96 (MQ=255)
                         gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGaa                                              >  2:13238161/1‑96 (MQ=255)
                         gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGaa                                              <  2:5625116/96‑1 (MQ=255)
                         gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGaa                                              >  1:5021815/1‑96 (MQ=255)
                           ttcaaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAAt                                            <  2:10664278/92‑1 (MQ=255)
                           ttcaaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAAt                                            <  1:1861003/92‑1 (MQ=255)
                           ttcaaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAAt                                            >  1:3199527/5‑96 (MQ=255)
                            tcaaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGTTTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATg                                           >  1:2924063/4‑96 (MQ=255)
                            tcaaaaGGCCATGCGCTGGAAGCAGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATg                                           <  1:10058740/93‑1 (MQ=255)
                             caaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAG‑CATGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGc                                          >  1:2711779/3‑96 (MQ=255)
                              aaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAAGGACGCGGAAATGCt                                         >  1:1281826/2‑96 (MQ=255)
                              aaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGca                                         <  1:4128963/95‑2 (MQ=255)
                              aaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCt                                         >  1:3482551/2‑96 (MQ=255)
                                aaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGt                                       <  1:5889603/96‑1 (MQ=255)
                                aaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGt                                       <  1:4934091/96‑1 (MQ=255)
                                 aGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGTCTG‑GTGACGGGATGAACGACGCCGAAATGCTGTc                                      <  1:9429715/96‑1 (MQ=255)
                                 aGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGtt                                      <  1:8861793/96‑2 (MQ=255)
                                 aGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTc                                      >  1:3023630/1‑96 (MQ=255)
                                 aGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTc                                      >  2:8011587/1‑96 (MQ=255)
                                 aGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTc                                      <  1:1435467/96‑1 (MQ=255)
                                 aGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTc                                      <  1:1745716/96‑1 (MQ=255)
                                 aGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTc                                      >  1:6322331/1‑96 (MQ=255)
                                 aGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTc                                      <  2:422780/96‑1 (MQ=255)
                                 aGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTc                                      <  2:13878938/96‑1 (MQ=255)
                                 aGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTc                                      >  2:1856400/1‑96 (MQ=255)
                                 aGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTc                                      >  1:4364937/1‑96 (MQ=255)
                                  ggCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTct                                     <  2:9449805/96‑2 (MQ=255)
                                  ggCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCg                                     <  2:8897623/96‑1 (MQ=255)
                                  ggCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCg                                     <  2:8494437/96‑1 (MQ=255)
                                                               actgg‑gCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAg        <  2:5353717/92‑1 (MQ=255)
                                                               actgg‑gCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAg        <  2:6198175/92‑1 (MQ=255)
                                                                ctgg‑gCTACAGCC‑TGATGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGt       >  1:1375344/4‑96 (MQ=255)
                                                                ctgg‑gCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGt       <  2:2115822/93‑1 (MQ=255)
                                                                ctgg‑gCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGt       >  2:12899592/4‑96 (MQ=255)
                                                                ctgg‑gCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGt       <  2:4202135/93‑1 (MQ=255)
                                                                ctgg‑gCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGt       >  2:3489403/4‑96 (MQ=255)
                                                                 tgg‑gCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTg      >  2:14020536/3‑96 (MQ=255)
                                                                 tgg‑gCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTg      >  1:9543118/3‑96 (MQ=255)
                                                                 tgg‑gCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTg      >  2:7302958/3‑96 (MQ=255)
                                                                  gg‑gCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTgc     <  1:8225197/95‑2 (MQ=255)
                                                                   g‑gCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTgcg    >  1:7076684/1‑94 (MQ=255)
                                                                   g‑gCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTgcg    <  1:13238161/96‑3 (MQ=255)
                                                                   g‑gCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTgcg    >  2:2281011/1‑94 (MQ=255)
                                                                   g‑gCTACAG‑CGTGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTgcg    <  2:13437271/96‑3 (MQ=255)
                                                                     gCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTgcgc   <  2:7099420/96‑4 (MQ=255)
                                                                     gCTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTgcgc   <  2:3449894/96‑4 (MQ=255)
                                                                      cTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGCAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTgcgca  <  1:517515/96‑5 (MQ=255)
                                                                      cTACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTgcgca  >  2:198723/1‑92 (MQ=255)
                                                                       tACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCAttgt              <  1:14250077/83‑3 (MQ=255)
                                                                       tACAGCC‑TGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTAt              >  2:14250077/1‑83 (MQ=255)
                                                                            |                                                                                           
CTGTCTGGAAGTGATGGCGGGCGGCGTTTC‑AAAGGCCATGCGCTGGAAG‑GCTGGCG‑‑‑‑AACT‑G‑GCTACAG‑C‑TGAAGGATTG‑ATTGCGT‑T‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGAAA‑GCTGCATTATGGGCAGTGGCCA  >  NC_000913/4008343‑4008494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAAGTGATGGCGGGCGGCGTTTC‑AAAGGCCATGCGCTGGAAG‑GC‑‑TGGCG‑‑‑‑AA‑‑‑‑CT‑GGCTACAG‑C‑TGAAGGATTG‑ATTGCG‑‑TT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑AAAG‑CTGCATTATGGGCAGTGGCCACCAGCGTCTGAA  >  NC_000913/4008350‑4008506
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NNNGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGAT                                                                          <  SRR4302518.11741891/101‑1 (MQ=18)
GAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGAT                                                                          <  SRR4302518.1114364/101‑1 (MQ=23)
 NNNTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATG                                                                         <  SRR4302518.13664866/101‑1 (MQ=17)
 NNNTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATG                                                                         <  SRR4302518.12262059/101‑1 (MQ=17)
   GTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAA                                                                       <  SRR4302518.4579327/101‑1 (MQ=18)
    TGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAAC                                                                      <  SRR4302518.3295537/101‑1 (MQ=18)
    TGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAAC                                                                      <  SRR4302518.1641741/101‑1 (MQ=18)
    TGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAAC                                                                      <  SRR4302518.5430644/101‑1 (MQ=18)
    GGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAAC                                                                      <  SRR4302518.4720110/101‑1 (MQ=17)
     GATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACG                                                                     <  SRR4302518.4547432/101‑1 (MQ=18)
     GATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACG                                                                     <  SRR4302518.2106652/101‑1 (MQ=18)
      ATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGA                                                                    <  SRR4302518.7333498/101‑1 (MQ=18)
        GGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACG                                                                  <  SRR4302518.1880780/101‑1 (MQ=18)
         GCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGC                                                                 <  SRR4302518.971059/101‑1 (MQ=18)
          CGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCC                                                                <  SRR4302518.7255353/101‑1 (MQ=18)
          CGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCC                                                                <  SRR4302518.3386956/101‑1 (MQ=18)
           GGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCG                                                               >  SRR4302518.6598371/1‑101 (MQ=18)
            GGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGA                                                              >  SRR4302518.4514232/1‑101 (MQ=18)
             GCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAA                                                             <  SRR4302518.738790/101‑1 (MQ=18)
               GGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAAT                                                           >  SRR4302518.2801977/1‑101 (MQ=18)
               GGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAAT                                                           <  SRR4302518.1944757/101‑1 (MQ=18)
                 CGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGC                                                         >  SRR4302518.4500920/1‑101 (MQ=20)
                 CGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGC                                                         >  SRR4302518.4950324/1‑101 (MQ=20)
                  nnntcaAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCT                                                        <  SRR4302518.13996534/95‑1 (MQ=23)
                  nnntcaAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCT                                                        <  SRR4302518.7710979/95‑1 (MQ=23)
                  GTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATNNN                                                        >  SRR4302518.14587260/1‑101 (MQ=17)
                  GTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATNNN                                                        >  SRR4302518.9944202/1‑101 (MQ=17)
                  GTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATNNN                                                        >  SRR4302518.11622897/1‑101 (MQ=17)
                  GTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATNNN                                                        >  SRR4302518.7959043/1‑101 (MQ=17)
                  GTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATNNN                                                        >  SRR4302518.10207111/1‑101 (MQ=17)
                  GTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCT                                                        >  SRR4302518.3639771/1‑101 (MQ=22)
                  GTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCT                                                        <  SRR4302518.4019725/101‑1 (MQ=22)
                  GTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCT                                                        >  SRR4302518.5282400/1‑101 (MQ=22)
                  GTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCG                                                        >  SRR4302518.1358678/1‑101 (MQ=20)
                  GTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCA                                                        <  SRR4302518.4329339/101‑1 (MQ=20)
                   nnncaAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTG                                                       <  SRR4302518.14914176/96‑1 (MQ=24)
                   gttcaAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTG                                                       <  SRR4302518.3964086/96‑1 (MQ=24)
                   TTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTG                                                       >  SRR4302518.3015330/1‑101 (MQ=23)
                    nnnaAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGT                                                      <  SRR4302518.12117278/97‑1 (MQ=26)
                    TTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGT                                                      >  SRR4302518.3348434/1‑101 (MQ=25)
                    TTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGT                                                      <  SRR4302518.6188071/101‑1 (MQ=25)
                    TTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGT                                                      <  SRR4302518.5124865/101‑1 (MQ=25)
                    TTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGT                                                      <  SRR4302518.5190948/101‑1 (MQ=25)
                    TTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGT                                                      <  SRR4302518.5241394/101‑1 (MQ=25)
                    NNN‑AAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCGTCTG‑‑GTGACGGGATGAACGACGCCGAAATGCTGTC                                                     <  SRR4302518.9885816/101‑1 (MQ=18)
                    NNN‑AAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTT                                                     <  SRR4302518.9291507/101‑1 (MQ=25)
                     tcaAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGTTTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTC                                                     >  SRR4302518.3061156/4‑101 (MQ=19)
                     tcaAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTNNN                                                     >  SRR4302518.14030986/4‑101 (MQ=22)
                     tcaAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTNNN                                                     >  SRR4302518.9055649/4‑101 (MQ=22)
                     tcaAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTC                                                     <  SRR4302518.1823641/98‑1 (MQ=27)
                     tcaAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTC                                                     <  SRR4302518.3146525/98‑1 (MQ=27)
                     tcaAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTC                                                     <  SRR4302518.1495681/98‑1 (MQ=27)
                      nnntttcaaaaggccatgcgctggaagcagtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATG                                                          <  SRR4302518.10535739/57‑1 (MQ=27)
                      caAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCG                                                    <  SRR4302518.295536/99‑1 (MQ=29)
                      caAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAG‑CATGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCG                                                    >  SRR4302518.2840817/3‑101 (MQ=29)
                      A‑AAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGgcgg                                                   >  SRR4302518.3644569/1‑97 (MQ=24)
                      A‑AAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTNNN                                                   >  SRR4302518.13107698/1‑101 (MQ=25)
                      A‑AAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGAAGCCGAAATGCTGTCGA                                                   >  SRR4302518.2118040/1‑101 (MQ=22)
                       cgtttcaaaaggccatgcgctggaagcgggggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGC                                                         >  SRR4302518.3390211/44‑101 (MQ=29)
                        AAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCNNN                                                  >  SRR4302518.8283886/1‑101 (MQ=27)
                         AAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATG                                                 <  SRR4302518.897486/101‑1 (MQ=32)
                         AAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATG                                                 <  SRR4302518.6669245/101‑1 (MQ=32)
                         AAGGCCATGCGCTGG‑AG‑GCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCGGAAATGCTGTCGATG                                                 >  SRR4302518.4422904/1‑101 (MQ=23)
                          AGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG                                                >  SRR4302518.3165368/1‑101 (MQ=34)
                          AGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG                                                >  SRR4302518.6644713/1‑101 (MQ=34)
                          AGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG                                                >  SRR4302518.4583866/1‑101 (MQ=34)
                          AGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG                                                <  SRR4302518.466545/101‑1 (MQ=34)
                          AGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG                                                >  SRR4302518.110381/1‑101 (MQ=34)
                          AGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG                                                >  SRR4302518.5495458/1‑101 (MQ=34)
                           nnnaaaggccatgcgctggaatcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTC                                                     <  SRR4302518.13406422/62‑1 (MQ=38)
                           GGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC                                               <  SRR4302518.2965700/101‑1 (MQ=36)
                             CCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGN‑‑NN                                           >  SRR4302518.10510098/1‑101 (MQ=34)
                             CCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGN‑‑NN                                           >  SRR4302518.11275641/1‑101 (MQ=34)
                             CCATGCGCTGGAAGCGG‑‑TGGCG‑‑‑‑AAGCATCTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GG                                           <  SRR4302518.7312009/101‑1 (MQ=39)
                              nnnggccatgcgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGAT                                                  <  SRR4302518.10859609/65‑1 (MQ=45)
                              nnnggccatgcgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGAT                                                  <  SRR4302518.8713515/65‑1 (MQ=45)
                              nnnggccatgcgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGAC                                                  <  SRR4302518.9269653/65‑1 (MQ=42)
                               nnngccatgcgctggaggcggtggcgaagaagctgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATG                                                 <  SRR4302518.8374469/66‑1 (MQ=47)
                               nnngccatgcgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATG                                                 <  SRR4302518.7403012/66‑1 (MQ=47)
                               nnngccatgcgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATG                                                 <  SRR4302518.10829477/66‑1 (MQ=47)
                                aggccatgcgctggaagcggtggcgaagaatctgggatacagcC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG                                                <  SRR4302518.5735726/58‑1 (MQ=53)
                                 gcccatgcgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATNNN                                               >  SRR4302518.12394668/34‑101 (MQ=43)
                                   nnntgcgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GG                                           <  SRR4302518.8227591/70‑1 (MQ=55)
                                   nnntgcgcgggacgcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GG                                           <  SRR4302518.10540020/70‑1 (MQ=55)
                                   ncatgcgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GN                                           <  SRR4302518.3698979/70‑1 (MQ=52)
                                   ccatgcgctggaagcagtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GG                                           <  SRR4302518.2496556/70‑1 (MQ=55)
                                   caatgcgatggaagcggtggagaagaatctgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GG                                           <  SRR4302518.4192643/70‑1 (MQ=55)
                                    catgcgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑NNN                                          >  SRR4302518.8956223/31‑101 (MQ=49)
                                    catgcgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG                                          <  SRR4302518.5947678/71‑1 (MQ=57)
                                    catgcgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG                                          <  SRR4302518.2792056/71‑1 (MQ=57)
                                    catgcgctggaagcgggggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG                                          >  SRR4302518.6021392/31‑101 (MQ=57)
                                     nnncgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑G                                       <  SRR4302518.11399308/72‑1 (MQ=56)
                                     atgcgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GNN‑‑N                                       >  SRR4302518.13704374/30‑101 (MQ=51)
                                     atgcgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑G                                       >  SRR4302518.1875309/30‑101 (MQ=56)
                                     atgcgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑G                                       >  SRR4302518.5222732/30‑101 (MQ=56)
                                     atgcgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑G                                       >  SRR4302518.5623195/30‑101 (MQ=56)
                                     atgcgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCGGTCGATGGCannn                                           >  SRR4302518.12476121/30‑97 (MQ=37)
                                      tgcgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGN‑‑NN                                      >  SRR4302518.12586831/29‑101 (MQ=53)
                                      tgcgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑GA                                      <  SRR4302518.5161957/73‑1 (MQ=56)
                                      tgcgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑GA                                      <  SRR4302518.5493192/73‑1 (MQ=56)
                                       gcgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑NNN                                     >  SRR4302518.9735495/28‑101 (MQ=55)
                                       gagctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑NNN                                     >  SRR4302518.12239215/28‑101 (MQ=55)
                                        nnntggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaca                                      <  SRR4302518.7929711/75‑5 (MQ=57)
                                        cgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑‑AAA                                     <  SRR4302518.5176302/75‑1 (MQ=54)
                                        cgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑‑AAA                                     <  SRR4302518.1945444/75‑1 (MQ=54)
                                        cgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑‑AAA                                     <  SRR4302518.1568600/75‑1 (MQ=54)
                                         gctggaagcggtgtcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgannn                                     >  SRR4302518.14053388/26‑96 (MQ=57)
                                         gctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑‑AAAG                                    >  SRR4302518.5021749/26‑101 (MQ=53)
                                         gctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑‑AAAG                                    >  SRR4302518.605793/26‑101 (MQ=53)
                                         gctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑‑AAAG                                    >  SRR4302518.3561432/26‑101 (MQ=53)
                                         gctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑‑AAAG                                    <  SRR4302518.464257/76‑1 (MQ=53)
                                         gctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgannn                                     >  SRR4302518.9466462/26‑96 (MQ=57)
                                          nnngaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaagg                                    <  SRR4302518.9205039/77‑7 (MQ=57)
                                           tggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaannn                                   >  SRR4302518.11192794/24‑94 (MQ=57)
                                            nnnagcggtggcgaagaaactgggctactgcC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggct                                  <  SRR4302518.10565195/70‑9 (MQ=60)
                                            ggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaagnnn                                  >  SRR4302518.7934335/23‑93 (MQ=57)
                                            ggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggct                                  <  SRR4302518.7188233/79‑9 (MQ=57)
                                            gaaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaagnnn                                  >  SRR4302518.11809035/23‑93 (MQ=57)
                                             gaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggnnn                                 >  SRR4302518.14367837/22‑92 (MQ=57)
                                             gaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggctg                                 >  SRR4302518.5690821/22‑92 (MQ=57)
                                             gaagcggtgccgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggctg                                 <  SRR4302518.7039355/80‑10 (MQ=57)
                                              aagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggcnnn                                >  SRR4302518.9398353/21‑91 (MQ=57)
                                               agcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGCCGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCA                              <  SRR4302518.6350775/82‑1 (MQ=38)
                                               agcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCA                              <  SRR4302518.5972639/82‑1 (MQ=50)
                                               agcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCA                              <  SRR4302518.594404/82‑1 (MQ=50)
                                                gcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaacacggnnn                              >  SRR4302518.10913046/19‑89 (MQ=57)
                                                 nnntggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATT                            <  SRR4302518.12103275/84‑1 (MQ=49)
                                                 gggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATT                            <  SRR4302518.3986437/84‑1 (MQ=49)
                                                 cggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGCTGGC‑‑GGGgaaaggctgcnnn                             >  SRR4302518.10129589/18‑88 (MQ=43)
                                                 cggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaacggctgcnnn                             >  SRR4302518.11535205/18‑88 (MQ=57)
                                                  ggtggcgaagaacctgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTA                           <  SRR4302518.63430/85‑1 (MQ=48)
                                                  ggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTA                           <  SRR4302518.1136692/85‑1 (MQ=48)
                                                  ggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGCATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTA                           <  SRR4302518.4727505/85‑1 (MQ=37)
                                                   nnnacgctcttccgatcttacagcC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggctgcattgt                           <  SRR4302518.14906990/77‑16 (MQ=60)
                                                   gtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTAT                          >  SRR4302518.6122901/16‑101 (MQ=48)
                                                   gtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTAT                          <  SRR4302518.5520491/86‑1 (MQ=48)
                                                   gtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTAT                          <  SRR4302518.102711/86‑1 (MQ=48)
                                                    tggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATG                         <  SRR4302518.4745017/87‑1 (MQ=49)
                                                    tggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATG                         >  SRR4302518.2718212/15‑101 (MQ=49)
                                                     nnngaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGG                        <  SRR4302518.12185701/88‑1 (MQ=51)
                                                     ggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGCTGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGACAGG‑CTGCATTATGG                        <  SRR4302518.2649679/88‑1 (MQ=36)
                                                     ggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGG                        <  SRR4302518.1521354/88‑1 (MQ=51)
                                                      nnnaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAATG‑CTGCATTATGGG                       <  SRR4302518.14526685/89‑1 (MQ=53)
                                                      nnnaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGG                       <  SRR4302518.7882123/89‑1 (MQ=53)
                                                      gcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGG                       >  SRR4302518.63966/13‑101 (MQ=53)
                                                       nnnagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGC                      <  SRR4302518.10049135/90‑1 (MQ=55)
                                                       nnnagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGC                      <  SRR4302518.9332267/90‑1 (MQ=55)
                                                       cgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGgggggaaaggcggagttatgggc                         >  SRR4302518.5659432/12‑78 (MQ=49)
                                                       cgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGC                      >  SRR4302518.2495117/12‑101 (MQ=55)
                                                        gaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGTC‑‑GGGGAAAGG‑CTGCATTATGagaa                     >  SRR4302518.498692/11‑97 (MQ=38)
                                                        gaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCA                     >  SRR4302518.1533440/11‑101 (MQ=57)
                                                        gaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCA                     >  SRR4302518.2738645/11‑101 (MQ=57)
                                                         AA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGggcg                >  SRR4302518.782287/1‑97 (MQ=56)
                                                         AA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTNNN                >  SRR4302518.14097003/1‑101 (MQ=56)
                                                          agaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCNNN                   >  SRR4302518.12870259/9‑101 (MQ=53)
                                                          agaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCNNN                   >  SRR4302518.10344564/9‑101 (MQ=53)
                                                           nnnactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTC                  <  SRR4302518.11887451/94‑1 (MQ=60)
                                                           gaaccgggccacgagcC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAAGGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTG                  <  SRR4302518.1485561/85‑1 (MQ=53)
                                                           gaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGtcannn                  >  SRR4302518.8312034/8‑95 (MQ=51)
                                                           gaaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTG                  >  SRR4302518.5432985/8‑101 (MQ=60)
                                                            nnnctgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGC                 <  SRR4302518.8631361/95‑1 (MQ=60)
                                                            aaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGC                 <  SRR4302518.7027038/95‑1 (MQ=60)
                                                            aaactgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGC                 >  SRR4302518.5804999/7‑101 (MQ=60)
                                                             nnntgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGcg                <  SRR4302518.13848542/96‑3 (MQ=60)
                                                               ctgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGCAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGcgca              <  SRR4302518.537833/98‑5 (MQ=50)
                                                               ctgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGcnnn              >  SRR4302518.7574363/4‑97 (MQ=60)
                                                                tgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGcgnnn             >  SRR4302518.10005154/3‑96 (MQ=60)
                                                                tgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGcgnnn             >  SRR4302518.14710225/3‑96 (MQ=60)
                                                                tgGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGcgcac             >  SRR4302518.1980913/3‑96 (MQ=60)
                                                                  nnntacagcC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCA           <  SRR4302518.15093088/92‑1 (MQ=60)
                                                                  GGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGcgcannn           >  SRR4302518.7450506/1‑94 (MQ=60)
                                                                  GGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCA           <  SRR4302518.5751923/101‑1 (MQ=58)
                                                                  GGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCA           <  SRR4302518.3297311/101‑1 (MQ=58)
                                                                   nnnacagcC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAG          <  SRR4302518.9739056/93‑1 (MQ=60)
                                                                   GCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAG          >  SRR4302518.4010129/1‑101 (MQ=56)
                                                                    nnncagcC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGC         <  SRR4302518.13842606/94‑1 (MQ=60)
                                                                     nnnagcC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCG        <  SRR4302518.14124532/95‑1 (MQ=59)
                                                                     TACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGggcgcacgagcg        >  SRR4302518.3822468/1‑89 (MQ=58)
                                                                     TACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGcgcaccannn        >  SRR4302518.8915013/1‑91 (MQ=60)
                                                                     TACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCG        >  SRR4302518.461668/1‑101 (MQ=56)
                                                                      nnngcC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGT       <  SRR4302518.12139774/96‑1 (MQ=60)
                                                                      ACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑‑AAAGCCTGCATTATGGGCAGTccgcaccagnnn       >  SRR4302518.13160693/1‑89 (MQ=58)
                                                                      ACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGGCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGT       >  SRR4302518.4245254/1‑101 (MQ=47)
                                                                       aagcC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCNNN      >  SRR4302518.11867277/5‑101 (MQ=58)
                                                                        agcC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGNNN     >  SRR4302518.8457010/4‑101 (MQ=57)
                                                                         gcC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGG‑‑AAGG‑CTGCATTATGGGCAGTGCGCACCAGCGGCTG    >  SRR4302518.3728394/3‑101 (MQ=57)
                                                                         C‑C‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGA   >  SRR4302518.6023663/1‑101 (MQ=60)
                                                                         C‑C‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGA   >  SRR4302518.5247632/1‑101 (MQ=60)
                                                                         C‑C‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCNNN   >  SRR4302518.14943133/1‑101 (MQ=60)
                                                                           C‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAA  >  SRR4302518.4965661/1‑101 (MQ=60)
                                                                           C‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGCGCGCACCAGCGTCTNNN  >  SRR4302518.11911103/1‑101 (MQ=52)
                                                                           C‑TGAAGGAATGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAA  >  SRR4302518.6822864/1‑101 (MQ=60)
                                                                           |                                                                                                          
GAAGTGATGGCGGGCGGCGTTTC‑AAAGGCCATGCGCTGGAAG‑GC‑‑TGGCG‑‑‑‑AA‑‑‑‑CT‑GGCTACAG‑C‑TGAAGGATTG‑ATTGCG‑‑TT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑AAAG‑CTGCATTATGGGCAGTGGCCACCAGCGTCTGAA  >  NC_000913/4008350‑4008506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑