Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 245 579.6 28867798 97.7% 28203838 96.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,008,421 +T coding (504/516 nt) b4555 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,008,4211.T99.7% 1096.0 / ‑6.4 284L168F (TTG→TTT) b4555hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (1/0);  major base T (137/145);  minor base A (1/0);  total (139/145)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 289 total aligned reads displayed.

CTGTCTGGAAGTGATGGCGGGCGGCGTTTC‑AAAGGCCATGCGCTGGAAG‑GCTGGCG‑‑‑‑AACT‑GGCTACAG‑C‑TGAAGGATT‑G‑ATTGC‑G‑TT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GGGAAA‑GCTGCATTATGGGCAGTGGC‑CACCAGCGTCTGAA  >  NC_000913/4008343‑4008506
                                                                                         |                                                                                             
cTGTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑tt                                                                                     <  2:3761128/96‑1 (MQ=255)
 tGTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑ttt                                                                                    <  1:5021437/96‑2 (MQ=255)
 tGTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑ttt                                                                                    <  2:5615949/96‑2 (MQ=255)
 tGTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑ttt                                                                                    <  2:2926582/96‑2 (MQ=255)
 tGTCTGGAAGTGATGGCGGGCGGAGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑ttt                                                                                    <  1:12583935/96‑2 (MQ=255)
  gTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑tttg                                                                                   <  1:9864495/96‑3 (MQ=255)
   tCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑tttgg                                                                                  >  1:3449894/1‑93 (MQ=255)
   tCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑tttgg                                                                                  <  2:9061588/96‑4 (MQ=255)
   tCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑tttgg                                                                                  <  2:8962905/96‑4 (MQ=255)
   tCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑tttgg                                                                                  >  2:7833193/1‑93 (MQ=255)
    cTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑tttggt                                                                                 <  2:7567338/96‑5 (MQ=255)
    cTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑tttggt                                                                                 >  2:446608/1‑92 (MQ=255)
           tGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGa                                                                          >  2:5270993/1‑96 (MQ=255)
            gATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑GCTTG‑GTGACGGGAt                                                                         <  1:10980536/96‑1 (MQ=255)
            gATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAG‑CATGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGAt                                                                         <  2:10216733/96‑1 (MQ=255)
             aTGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATg                                                                        <  2:354043/96‑1 (MQ=255)
             aTGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATg                                                                        <  1:11708699/96‑1 (MQ=255)
              tGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGAtcg                                                                       >  1:12886350/1‑94 (MQ=255)
               ggcgggcgGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGaa                                                                      <  1:13237122/96‑1 (MQ=255)
                gcgggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAac                                                                     <  1:3148361/96‑1 (MQ=255)
                gcgggcggCGTTTCAAAAGGACATGCCCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAac                                                                     <  1:7028547/96‑1 (MQ=255)
                 cgggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGCAGGATT‑GTAGTGC‑G‑TTTGGTGACGGGATGAacg                                                                    >  1:10184571/1‑96 (MQ=255)
                 cgggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAacg                                                                    <  2:2577626/96‑1 (MQ=255)
                 cgggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAacg                                                                    >  1:13181770/1‑96 (MQ=255)
                 cgggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGA‑G‑TTTGGTGACGGGATGAacg                                                                    >  2:9604794/1‑96 (MQ=255)
                  gggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAacga                                                                   >  2:6681137/1‑96 (MQ=255)
                  gggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAacga                                                                   >  2:13250770/1‑96 (MQ=255)
                  gggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAacga                                                                   >  2:10446427/1‑96 (MQ=255)
                  gggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAacga                                                                   >  1:6278348/1‑96 (MQ=255)
                  gggcggCGTTTCAAAACGCCATGAGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAacga                                                                   <  1:10357255/96‑1 (MQ=255)
                   gtcggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAacgac                                                                  <  1:12859185/94‑1 (MQ=255)
                   ggcggcGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAacgac                                                                  >  1:4300846/1‑96 (MQ=255)
                   ggcggcGTTTCAAAAGGCCATGCGCTGGAAGCGGGGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAacgac                                                                  >  1:1566776/1‑96 (MQ=255)
                    gcggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAacgacg                                                                 <  2:12997738/96‑1 (MQ=255)
                     cggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAAcgccgc                                                                <  1:781157/96‑1 (MQ=255)
                     cggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGc                                                                <  2:6956805/96‑1 (MQ=255)
                      ggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGcc                                                               <  1:3236505/96‑1 (MQ=255)
                      ggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGcc                                                               >  1:7394350/1‑96 (MQ=255)
                      ggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGcc                                                               >  1:2674379/1‑96 (MQ=255)
                      ggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGcc                                                               <  1:6889931/96‑1 (MQ=255)
                      ggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGATACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGcc                                                               <  1:11448052/96‑1 (MQ=255)
                      ggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGACGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GAATTGC‑G‑TTTGGTGACGGGATGAACGACGcc                                                               >  2:14090384/1‑96 (MQ=255)
                        cgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGa                                                             <  2:11320077/96‑1 (MQ=255)
                         gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGaa                                                            >  2:7698105/1‑96 (MQ=255)
                         gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGaa                                                            >  1:1305043/1‑96 (MQ=255)
                         gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGaa                                                            <  2:3393939/96‑1 (MQ=255)
                         gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGaa                                                            >  1:9484328/1‑96 (MQ=255)
                           ttcaaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAAt                                                          <  2:5010472/92‑1 (MQ=255)
                           ttcaaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAAt                                                          >  2:4221818/5‑96 (MQ=255)
                           ttcaaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAAt                                                          <  2:10664278/92‑1 (MQ=255)
                            tcaaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGTTT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATg                                                         >  1:2924063/4‑96 (MQ=255)
                              aaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGca                                                       <  1:4128963/95‑2 (MQ=255)
                              aaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCt                                                       <  2:2208879/95‑1 (MQ=255)
                              aaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCt                                                       >  2:1473711/2‑96 (MQ=255)
                               aaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGTCTAAATGCTg                                                      >  1:4755740/1‑96 (MQ=255)
                               aaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTg                                                      >  1:7888015/1‑96 (MQ=255)
                                aaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGt                                                     <  1:4934091/96‑1 (MQ=255)
                                 aGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGtt                                                    <  1:8861793/96‑2 (MQ=255)
                                 aGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTc                                                    >  2:8011587/1‑96 (MQ=255)
                                 aGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTc                                                    <  1:1745716/96‑1 (MQ=255)
                                 aGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTc                                                    >  1:4364937/1‑96 (MQ=255)
                                 aGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTc                                                    >  2:1856400/1‑96 (MQ=255)
                                 aGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTCACGGGATGAACGACGCCGAAATGCTGTc                                                    <  2:13877228/96‑1 (MQ=255)
                                  ggCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCg                                                   <  2:8494437/96‑1 (MQ=255)
                                                               actgggCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAg                     <  2:6198175/92‑1 (MQ=255)
                                                                ctgggCTACAGCC‑TGATGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGt                    >  1:1375344/4‑96 (MQ=255)
                                                                ctgggCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGt                    >  1:7196977/4‑96 (MQ=255)
                                                                 tgggCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTg                   >  1:1895113/3‑96 (MQ=255)
                                                                 tgggCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTg                   >  1:14066289/3‑96 (MQ=255)
                                                                 tgggCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTg                   >  2:7302958/3‑96 (MQ=255)
                                                                  gggCTACAGCC‑TGAAGGATT‑GTATTGCGG‑TTG‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTgc                  >  2:13879768/2‑95 (MQ=255)
                                                                    gCTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTgcgc                >  1:3829029/1‑93 (MQ=255)
                                                                     cTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGCAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTgcgca               <  1:517515/96‑5 (MQ=255)
                                                                     cTACAGCC‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTgcgca               >  2:198723/1‑92 (MQ=255)
                                                                        cagcc‑TGAAGGATT‑GTATTGCGG‑TTG‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCg‑cacca           >  2:1354987/5‑96 (MQ=255)
                                                                        aagcc‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCg‑cacca           >  1:11336053/5‑96 (MQ=255)
                                                                         agcc‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCG‑CACCAg          <  1:9290859/93‑1 (MQ=255)
                                                                         agcc‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCG‑CACCAg          >  1:8056151/4‑96 (MQ=255)
                                                                          gcc‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCG‑CACCAGc         >  1:3561769/3‑96 (MQ=255)
                                                                          gcc‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCG‑CACCAGc         >  2:7100183/3‑96 (MQ=255)
                                                                          gcc‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCG‑CACCAGc         <  2:12303177/94‑1 (MQ=255)
                                                                           cc‑TGAAGGTTT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCG‑CACCAGCg        >  1:5969647/2‑96 (MQ=255)
                                                                           cc‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCG‑CACCAGCg        >  2:3470877/2‑96 (MQ=255)
                                                                           cc‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCG‑CACCAGCg        >  1:14283081/2‑96 (MQ=255)
                                                                           cc‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCG‑CACCAGCg        >  1:5733034/2‑96 (MQ=255)
                                                                            c‑TGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCG‑CACCAGCGt       >  2:9355629/1‑96 (MQ=255)
                                                                              taaaGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCG‑CACCAGCGTc      >  1:10632480/3‑96 (MQ=255)
                                                                              tGAAGGTTT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCC‑GGCAAAGGCTGCATTATGGGCAGTGAG‑CACCAGCGTc      >  1:10000040/1‑96 (MQ=255)
                                                                              tGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCG‑CACCAGCgcc      >  2:5818650/1‑94 (MQ=255)
                                                                              tGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCG‑CACCAGCGTc      <  2:1903279/96‑1 (MQ=255)
                                                                              tGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCG‑CACCAGCGTc      >  2:12779655/1‑96 (MQ=255)
                                                                              tGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCG‑CACCAGCGTc      >  1:4647802/1‑96 (MQ=255)
                                                                              tGAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGC‑GAGGCGCACCAGCggc      >  1:6507775/1‑94 (MQ=255)
                                                                               gAATGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCG‑CACCAGCGTCt     >  2:10116508/1‑96 (MQ=255)
                                                                               gAAGGATTGG‑ATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTAGGGGCAGTGCG‑CACCAGCGTCt     >  1:7330067/1‑96 (MQ=255)
                                                                               gAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCG‑CACCAGCGTCt     <  2:9543819/96‑1 (MQ=255)
                                                                               gAAGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCG‑CACCAGCGTCt     >  2:443212/1‑96 (MQ=255)
                                                                                 aGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCG‑CACCAGCGTCTGa   >  1:1338132/1‑96 (MQ=255)
                                                                                 aGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCG‑CACCAGCGTCTGa   >  2:11942376/1‑96 (MQ=255)
                                                                                 aGGATT‑GTATTGC‑G‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCG‑CACCAGCGTCTGa   >  2:301481/1‑96 (MQ=255)
                                                                                         |                                                                                             
CTGTCTGGAAGTGATGGCGGGCGGCGTTTC‑AAAGGCCATGCGCTGGAAG‑GCTGGCG‑‑‑‑AACT‑GGCTACAG‑C‑TGAAGGATT‑G‑ATTGC‑G‑TT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GGGAAA‑GCTGCATTATGGGCAGTGGC‑CACCAGCGTCTGAA  >  NC_000913/4008343‑4008506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 221 total aligned reads displayed.
GAAGTGATGGCGGGCGGCGTTTC‑AAAGGCCATGCGCTGGAAG‑GC‑‑TGGCG‑‑‑‑AACT‑GGCTACAG‑C‑TGAAGGATT‑G‑ATTGCG‑‑TT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑AAAG‑CTGCATTATGGGCAGTGGC‑CACCAGCGTCTGAAAGACCTTCAT  >  NC_000913/4008350‑4008516
                                                                                   |                                                                                                          
NNNGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGAT                                                                                     <  SRR4302518.11741891/101‑1 (MQ=18)
GAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGAT                                                                                     <  SRR4302518.1114364/101‑1 (MQ=23)
 NNNTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATG                                                                                    <  SRR4302518.13664866/101‑1 (MQ=17)
 NNNTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATG                                                                                    <  SRR4302518.12262059/101‑1 (MQ=17)
   GTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAA                                                                                  <  SRR4302518.4579327/101‑1 (MQ=18)
    TGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAAC                                                                                 <  SRR4302518.1641741/101‑1 (MQ=18)
    TGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAAC                                                                                 <  SRR4302518.5430644/101‑1 (MQ=18)
    TGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAAC                                                                                 <  SRR4302518.3295537/101‑1 (MQ=18)
    GGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAAC                                                                                 <  SRR4302518.4720110/101‑1 (MQ=17)
     GATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACG                                                                                <  SRR4302518.4547432/101‑1 (MQ=18)
     GATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACG                                                                                <  SRR4302518.2106652/101‑1 (MQ=18)
      ATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGA                                                                               <  SRR4302518.7333498/101‑1 (MQ=18)
        GGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACG                                                                             <  SRR4302518.1880780/101‑1 (MQ=18)
         GCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGC                                                                            <  SRR4302518.971059/101‑1 (MQ=18)
          CGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCC                                                                           <  SRR4302518.7255353/101‑1 (MQ=18)
          CGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCC                                                                           <  SRR4302518.3386956/101‑1 (MQ=18)
           GGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCG                                                                          >  SRR4302518.6598371/1‑101 (MQ=18)
            GGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGA                                                                         >  SRR4302518.4514232/1‑101 (MQ=18)
             GCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAA                                                                        <  SRR4302518.738790/101‑1 (MQ=18)
               GGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAAT                                                                      <  SRR4302518.1944757/101‑1 (MQ=18)
               GGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAAT                                                                      >  SRR4302518.2801977/1‑101 (MQ=18)
                 CGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGC                                                                    >  SRR4302518.4950324/1‑101 (MQ=20)
                 CGTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGC                                                                    >  SRR4302518.4500920/1‑101 (MQ=20)
                  nnntttcaaaaggccatgcgctggaagcagtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATG                                                                     <  SRR4302518.10535739/57‑1 (MQ=27)
                  nnntcaAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCT                                                                   <  SRR4302518.13996534/95‑1 (MQ=23)
                  nnntcaAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCT                                                                   <  SRR4302518.7710979/95‑1 (MQ=23)
                  GTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATNNN                                                                   >  SRR4302518.11622897/1‑101 (MQ=17)
                  GTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATNNN                                                                   >  SRR4302518.10207111/1‑101 (MQ=17)
                  GTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATNNN                                                                   >  SRR4302518.7959043/1‑101 (MQ=17)
                  GTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATNNN                                                                   >  SRR4302518.14587260/1‑101 (MQ=17)
                  GTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCT                                                                   <  SRR4302518.4019725/101‑1 (MQ=22)
                  GTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCT                                                                   >  SRR4302518.5282400/1‑101 (MQ=22)
                  GTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCG                                                                   >  SRR4302518.1358678/1‑101 (MQ=20)
                  GTTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCA                                                                   <  SRR4302518.4329339/101‑1 (MQ=20)
                   nnncaAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTG                                                                  <  SRR4302518.14914176/96‑1 (MQ=24)
                   gttcaAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTG                                                                  <  SRR4302518.3964086/96‑1 (MQ=24)
                   cgtttcaaaaggccatgcgctggaagcgggggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGC                                                                    >  SRR4302518.3390211/44‑101 (MQ=29)
                    nnnaAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGT                                                                 <  SRR4302518.12117278/97‑1 (MQ=26)
                    TTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGT                                                                 <  SRR4302518.5241394/101‑1 (MQ=25)
                    TTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGT                                                                 <  SRR4302518.5190948/101‑1 (MQ=25)
                    TTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGT                                                                 <  SRR4302518.6188071/101‑1 (MQ=25)
                    TTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGT                                                                 >  SRR4302518.3348434/1‑101 (MQ=25)
                    NNN‑AAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCGTCTG‑‑GTGACGGGATGAACGACGCCGAAATGCTGTC                                                                <  SRR4302518.9885816/101‑1 (MQ=18)
                    NNN‑AAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTT                                                                <  SRR4302518.9291507/101‑1 (MQ=25)
                     tcaAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGTTT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTC                                                                >  SRR4302518.3061156/4‑101 (MQ=19)
                     tcaAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTNNN                                                                >  SRR4302518.14030986/4‑101 (MQ=22)
                     tcaAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTNNN                                                                >  SRR4302518.9055649/4‑101 (MQ=22)
                     tcaAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTC                                                                <  SRR4302518.3146525/98‑1 (MQ=27)
                     tcaAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTC                                                                <  SRR4302518.1495681/98‑1 (MQ=27)
                      caAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCG                                                               <  SRR4302518.295536/99‑1 (MQ=29)
                      caAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAG‑CATGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCG                                                               >  SRR4302518.2840817/3‑101 (MQ=29)
                      A‑AAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGgcgg                                                              >  SRR4302518.3644569/1‑97 (MQ=24)
                      A‑AAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTNNN                                                              >  SRR4302518.13107698/1‑101 (MQ=25)
                      A‑AAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGAAGCCGAAATGCTGTCGA                                                              >  SRR4302518.2118040/1‑101 (MQ=22)
                       nnnaaaggccatgcgctggaatcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTC                                                                <  SRR4302518.13406422/62‑1 (MQ=38)
                        AAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCNNN                                                             >  SRR4302518.8283886/1‑101 (MQ=27)
                         AAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATG                                                            <  SRR4302518.6669245/101‑1 (MQ=32)
                         AAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATG                                                            <  SRR4302518.897486/101‑1 (MQ=32)
                         AAGGCCATGCGCTGG‑AG‑GCGGTGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCGGAAATGCTGTCGATG                                                            >  SRR4302518.4422904/1‑101 (MQ=23)
                          nnnggccatgcgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGAT                                                             <  SRR4302518.10859609/65‑1 (MQ=45)
                          nnnggccatgcgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGAT                                                             <  SRR4302518.8713515/65‑1 (MQ=45)
                          AGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG                                                           >  SRR4302518.5495458/1‑101 (MQ=34)
                          AGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG                                                           >  SRR4302518.6644713/1‑101 (MQ=34)
                          AGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG                                                           >  SRR4302518.4583866/1‑101 (MQ=34)
                          AGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG                                                           >  SRR4302518.3165368/1‑101 (MQ=34)
                          AGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG                                                           >  SRR4302518.110381/1‑101 (MQ=34)
                           nnngccatgcgctggaggcggtggcgaagaagctgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATG                                                            <  SRR4302518.8374469/66‑1 (MQ=47)
                           nnngccatgcgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATG                                                            <  SRR4302518.7403012/66‑1 (MQ=47)
                           nnngccatgcgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATG                                                            <  SRR4302518.10829477/66‑1 (MQ=47)
                           GGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC                                                          <  SRR4302518.2965700/101‑1 (MQ=36)
                            aggccatgcgctggaagcggtggcgaagaatctgggatacagcC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG                                                           <  SRR4302518.5735726/58‑1 (MQ=53)
                             gcccatgcgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATNNN                                                          >  SRR4302518.12394668/34‑101 (MQ=43)
                             CCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGN‑‑NN                                                      >  SRR4302518.11275641/1‑101 (MQ=34)
                             CCATGCGCTGGAAGCGG‑‑TGGCGAAGAAACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGN‑‑NN                                                      >  SRR4302518.10510098/1‑101 (MQ=34)
                               nnntgcgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GG                                                      <  SRR4302518.8227591/70‑1 (MQ=55)
                               nnntgcgcgggacgcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GG                                                      <  SRR4302518.10540020/70‑1 (MQ=55)
                               ncatgcgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GN                                                      <  SRR4302518.3698979/70‑1 (MQ=52)
                               ccatgcgctggaagcagtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GG                                                      <  SRR4302518.2496556/70‑1 (MQ=55)
                                catgcgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑NNN                                                     >  SRR4302518.8956223/31‑101 (MQ=49)
                                catgcgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG                                                     <  SRR4302518.2792056/71‑1 (MQ=57)
                                catgcgctggaagcgggggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG                                                     >  SRR4302518.6021392/31‑101 (MQ=57)
                                 nnncgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑G                                                  <  SRR4302518.11399308/72‑1 (MQ=56)
                                 atgcgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GNN‑‑N                                                  >  SRR4302518.13704374/30‑101 (MQ=51)
                                 atgcgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑G                                                  >  SRR4302518.1875309/30‑101 (MQ=56)
                                 atgcgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑G                                                  >  SRR4302518.5623195/30‑101 (MQ=56)
                                 atgcgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑G                                                  >  SRR4302518.5222732/30‑101 (MQ=56)
                                 atgcgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCGGTCGATGGCannn                                                      >  SRR4302518.12476121/30‑97 (MQ=37)
                                  tgcgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGN‑‑NN                                                 >  SRR4302518.12586831/29‑101 (MQ=53)
                                  tgcgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑GA                                                 <  SRR4302518.5161957/73‑1 (MQ=56)
                                   gcgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑NNN                                                >  SRR4302518.9735495/28‑101 (MQ=55)
                                   gagctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑NNN                                                >  SRR4302518.12239215/28‑101 (MQ=55)
                                    nnntggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaca                                                 <  SRR4302518.7929711/75‑5 (MQ=57)
                                    cgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑‑AAA                                                <  SRR4302518.1945444/75‑1 (MQ=54)
                                    cgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑‑AAA                                                <  SRR4302518.5176302/75‑1 (MQ=54)
                                    cgctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑‑AAA                                                <  SRR4302518.1568600/75‑1 (MQ=54)
                                     gctggaagcggtgtcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgannn                                                >  SRR4302518.14053388/26‑96 (MQ=57)
                                     gctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑‑AAAG                                               >  SRR4302518.3561432/26‑101 (MQ=53)
                                     gctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑‑AAAG                                               >  SRR4302518.5021749/26‑101 (MQ=53)
                                     gctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑‑AAAG                                               <  SRR4302518.464257/76‑1 (MQ=53)
                                     gctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑‑AAAG                                               >  SRR4302518.605793/26‑101 (MQ=53)
                                     gctggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgannn                                                >  SRR4302518.9466462/26‑96 (MQ=57)
                                      nnngaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaagg                                               <  SRR4302518.9205039/77‑7 (MQ=57)
                                       tggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaannn                                              >  SRR4302518.11192794/24‑94 (MQ=57)
                                        ggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaagnnn                                             >  SRR4302518.7934335/23‑93 (MQ=57)
                                        ggaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggct                                             <  SRR4302518.7188233/79‑9 (MQ=57)
                                        gaaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaagnnn                                             >  SRR4302518.11809035/23‑93 (MQ=57)
                                         gaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggnnn                                            >  SRR4302518.14367837/22‑92 (MQ=57)
                                         gaagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggctg                                            >  SRR4302518.5690821/22‑92 (MQ=57)
                                         gaagcggtgccgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggctg                                            <  SRR4302518.7039355/80‑10 (MQ=57)
                                          aagcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggcnnn                                           >  SRR4302518.9398353/21‑91 (MQ=57)
                                           agcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGCCGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCA                                         <  SRR4302518.6350775/82‑1 (MQ=38)
                                           agcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCA                                         <  SRR4302518.5972639/82‑1 (MQ=50)
                                           agcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCA                                         <  SRR4302518.594404/82‑1 (MQ=50)
                                            gcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaacacggnnn                                         >  SRR4302518.10913046/19‑89 (MQ=57)
                                             gggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATT                                       <  SRR4302518.3986437/84‑1 (MQ=49)
                                             cggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGCTGGC‑‑GGGgaaaggctgcnnn                                        >  SRR4302518.10129589/18‑88 (MQ=43)
                                             cggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaacggctgcnnn                                        >  SRR4302518.11535205/18‑88 (MQ=57)
                                              ggtggcgaagaacctgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTA                                      <  SRR4302518.63430/85‑1 (MQ=48)
                                              ggtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGCATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTA                                      <  SRR4302518.4727505/85‑1 (MQ=37)
                                               nnnacgctcttccgatcttacagcC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggctgcattgt                                      <  SRR4302518.14906990/77‑16 (MQ=60)
                                               gtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTAT                                     <  SRR4302518.102711/86‑1 (MQ=48)
                                               gtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTAT                                     >  SRR4302518.6122901/16‑101 (MQ=48)
                                               gtggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTAT                                     <  SRR4302518.5520491/86‑1 (MQ=48)
                                                tggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATG                                    <  SRR4302518.4745017/87‑1 (MQ=49)
                                                tggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATG                                    >  SRR4302518.2718212/15‑101 (MQ=49)
                                                 nnngaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGG                                   <  SRR4302518.12185701/88‑1 (MQ=51)
                                                 ggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGCTGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGACAGG‑CTGCATTATGG                                   <  SRR4302518.2649679/88‑1 (MQ=36)
                                                 ggcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGG                                   <  SRR4302518.1521354/88‑1 (MQ=51)
                                                  nnnaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAATG‑CTGCATTATGGG                                  <  SRR4302518.14526685/89‑1 (MQ=53)
                                                  nnnaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGG                                  <  SRR4302518.7882123/89‑1 (MQ=53)
                                                  gcgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGG                                  >  SRR4302518.63966/13‑101 (MQ=53)
                                                   nnnagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGC                                 <  SRR4302518.9332267/90‑1 (MQ=55)
                                                   nnnagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGC                                 <  SRR4302518.10049135/90‑1 (MQ=55)
                                                   cgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGgggggaaaggcggagttatgggc                                    >  SRR4302518.5659432/12‑78 (MQ=49)
                                                   cgaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGC                                 >  SRR4302518.2495117/12‑101 (MQ=55)
                                                    gaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGTC‑‑GGGGAAAGG‑CTGCATTATGagaa                                >  SRR4302518.498692/11‑97 (MQ=38)
                                                    gaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCA                                >  SRR4302518.2738645/11‑101 (MQ=57)
                                                    gaagaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCA                                >  SRR4302518.1533440/11‑101 (MQ=57)
                                                     gaagaaactgggctacagcctggaggattgG‑ATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCG                                >  SRR4302518.1257162/31‑101 (MQ=57)
                                                      agaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCNNN                              >  SRR4302518.10344564/9‑101 (MQ=53)
                                                       nnnactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTC                             <  SRR4302518.11887451/94‑1 (MQ=60)
                                                       gaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGtcannn                             >  SRR4302518.8312034/8‑95 (MQ=51)
                                                       gaaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTG                             >  SRR4302518.5432985/8‑101 (MQ=60)
                                                        nnnctgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGC                            <  SRR4302518.8631361/95‑1 (MQ=60)
                                                        aaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGC                            >  SRR4302518.5804999/7‑101 (MQ=60)
                                                        aaactgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGC                            <  SRR4302518.7027038/95‑1 (MQ=60)
                                                         AACTGGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTNNN                           >  SRR4302518.14097003/1‑101 (MQ=56)
                                                           ctgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGCAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGcgca                         <  SRR4302518.537833/98‑5 (MQ=50)
                                                           ctgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGcnnn                         >  SRR4302518.7574363/4‑97 (MQ=60)
                                                            tgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGcgnnn                        >  SRR4302518.10005154/3‑96 (MQ=60)
                                                            tgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGcgnnn                        >  SRR4302518.14710225/3‑96 (MQ=60)
                                                            tgGGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGcgcac                        >  SRR4302518.1980913/3‑96 (MQ=60)
                                                              nnntacagcC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCG‑CACCA                     <  SRR4302518.15093088/92‑1 (MQ=60)
                                                              GGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGcgcannn                      >  SRR4302518.7450506/1‑94 (MQ=60)
                                                              GGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCG‑CACCA                     <  SRR4302518.3297311/101‑1 (MQ=58)
                                                              GGCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCG‑CACCA                     <  SRR4302518.5751923/101‑1 (MQ=58)
                                                               nnnacagcC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCG‑CACCAG                    <  SRR4302518.9739056/93‑1 (MQ=60)
                                                               GCTACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCG‑CACCAG                    >  SRR4302518.4010129/1‑101 (MQ=56)
                                                                nnncagcC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCG‑CACCAGC                   <  SRR4302518.13842606/94‑1 (MQ=60)
                                                                 nnnagcC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCG‑CACCAGCG                  <  SRR4302518.14124532/95‑1 (MQ=59)
                                                                 TACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGggcgcacgagcg                   >  SRR4302518.3822468/1‑89 (MQ=58)
                                                                 TACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGcgcaccannn                   >  SRR4302518.8915013/1‑91 (MQ=60)
                                                                 TACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCG‑CACCAGCG                  >  SRR4302518.461668/1‑101 (MQ=56)
                                                                  nnngcC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCG‑CACCAGCGT                 <  SRR4302518.12139774/96‑1 (MQ=60)
                                                                  ACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑‑AAAGCCTGCATTATGGGCAGTccgcaccagnnn                  >  SRR4302518.13160693/1‑89 (MQ=58)
                                                                  ACAGCC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGGCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCG‑CACCAGCGT                 >  SRR4302518.4245254/1‑101 (MQ=47)
                                                                   aagcC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCG‑CACCAGCNNN                >  SRR4302518.11867277/5‑101 (MQ=58)
                                                                     gcC‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGG‑‑AAGG‑CTGCATTATGGGCAGTGCG‑CACCAGCGGCTG              >  SRR4302518.3728394/3‑101 (MQ=57)
                                                                     C‑C‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCG‑CACCAGCGTCTGA             >  SRR4302518.5247632/1‑101 (MQ=60)
                                                                     C‑C‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCG‑CACCAGCGTCTGA             >  SRR4302518.6023663/1‑101 (MQ=60)
                                                                     C‑C‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCG‑CACCAGCGTCNNN             >  SRR4302518.14943133/1‑101 (MQ=60)
                                                                       C‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCG‑CACCAGCGTCTGAA            >  SRR4302518.4965661/1‑101 (MQ=60)
                                                                       C‑TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGCGCG‑CACCAGCGTCTNNN            >  SRR4302518.11911103/1‑101 (MQ=52)
                                                                       C‑TGAAGGAAT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCG‑CACCAGCGTCTGAA            >  SRR4302518.6822864/1‑101 (MQ=60)
                                                                         TGAAGGTTT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑CGGCAAAGG‑CTGCATTATGGGCAGTGAG‑CACCAGCGTCTcnnn           >  SRR4302518.10475560/1‑97 (MQ=45)
                                                                         TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCG‑CACCAGCGTCTGNNN           >  SRR4302518.11363911/1‑101 (MQ=60)
                                                                         TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCG‑CACCAGCGTCTGACA           >  SRR4302518.4892487/1‑101 (MQ=60)
                                                                         TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCG‑CACCAGCGTCTGAAA           >  SRR4302518.815220/1‑101 (MQ=60)
                                                                         TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGGGCG‑CACCAGCGTCTGAAA           >  SRR4302518.343684/1‑101 (MQ=58)
                                                                         TGAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGC‑GAGGCGCACCAGCGGCTGAAA           >  SRR4302518.6841188/1‑101 (MQ=45)
                                                                         TAAAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCG‑CACCAGCGTCTGNNN           >  SRR4302518.11132113/1‑101 (MQ=58)
                                                                         NNNAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCG‑CACCAGCGTCTGAAA           <  SRR4302518.7731361/101‑1 (MQ=60)
                                                                          NNNGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCG‑CACCAGCGTCTGAACG          <  SRR4302518.11600147/101‑1 (MQ=60)
                                                                          GAAGGATTGG‑ATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTAGGGGCAGTGCG‑CACCAGCGTCTGANNN          >  SRR4302518.7711107/1‑101 (MQ=52)
                                                                           AAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCG‑CACCAGCGTCTGAAAGC         >  SRR4302518.7334146/1‑101 (MQ=60)
                                                                           AAGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCG‑CACCAGCGTCTGAAAGA         <  SRR4302518.2537259/101‑1 (MQ=60)
                                                                            AGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCG‑CACCAGCGTCTGAAANNN        >  SRR4302518.9625538/1‑101 (MQ=60)
                                                                            AGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCG‑CACCAGCGTCTGAAANNN        >  SRR4302518.10684965/1‑101 (MQ=60)
                                                                            AGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCG‑CACCAGCGTCTGAAAGAC        >  SRR4302518.1393417/1‑101 (MQ=60)
                                                                            AGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCG‑CACCAGCGGCTGAAANNN        >  SRR4302518.13792003/1‑101 (MQ=52)
                                                                            AGGATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCGGTCGATGcaggggaaagtctgcgttatgggcatcgcgcaccagcgacagagagac            >  SRR4302518.911451/1‑53 (MQ=26)
                                                                              GATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCG‑CACCAGCGTCTGAAAGANNN      >  SRR4302518.9097783/1‑101 (MQ=60)
                                                                              GATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCG‑CACCAGCGTCTGAAAGACCT      <  SRR4302518.1304244/101‑1 (MQ=60)
                                                                              GATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCG‑CACAAGCGACTGAAAGACCT      >  SRR4302518.3925679/1‑101 (MQ=52)
                                                                               ATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCG‑CACCAGCGTCTGAAAGACCTT     >  SRR4302518.5959733/1‑101 (MQ=60)
                                                                               ATT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCG‑CACCAGCGTCTGAAAGACCTT     >  SRR4302518.1909594/1‑101 (MQ=60)
                                                                                TT‑GTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCG‑CACCAGCGTCTGAAAGACCTTC    <  SRR4302518.5004258/101‑1 (MQ=60)
                                                                                NN‑N‑ATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCG‑CACCAGCGTCTGAAAGACCTTTA   <  SRR4302518.12236479/101‑1 (MQ=60)
                                                                                NN‑N‑ATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCG‑CACCAGCGTCTGAAAGACCTTCA   <  SRR4302518.7735324/101‑1 (MQ=60)
                                                                                 N‑N‑NTTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCG‑CACCAGCGTCTGAAAGACCTTCAT  <  SRR4302518.9074244/101‑1 (MQ=60)
                                                                                   |                                                                                                          
GAAGTGATGGCGGGCGGCGTTTC‑AAAGGCCATGCGCTGGAAG‑GC‑‑TGGCG‑‑‑‑AACT‑GGCTACAG‑C‑TGAAGGATT‑G‑ATTGCG‑‑TT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑AAAG‑CTGCATTATGGGCAGTGGC‑CACCAGCGTCTGAAAGACCTTCAT  >  NC_000913/4008350‑4008516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑