Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,008,421 +T coding (504/516 nt) b4555 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,008,4211.T100.0% 676.7 / NA 186L168F (TTG→TTT) b4555hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (80/106);  total (80/106)

Only 100 of 190 total aligned reads displayed.

TCTGGAAGTGATGGCGGGCGGCGTTTC‑AAAGGCCATGCGCTGGAAG‑GCTGGCG‑‑‑‑AACT‑GGCTACAG‑CTGAAGGATTG‑ATTGC‑GTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑G‑‑AAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCT  >  NC_000913/4008346‑4008503
                                                                                    |                                                                                          
tCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑Gtt                                                                                   <  2:6362410/93‑1 (MQ=255)
tCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑Gtt                                                                                   <  1:7177307/93‑1 (MQ=255)
tCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑Gtt                                                                                   <  1:2396856/93‑1 (MQ=255)
 cTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑Gttt                                                                                  <  1:1148820/93‑2 (MQ=255)
 cTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑Gttt                                                                                  >  2:3112731/1‑92 (MQ=255)
  tGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑Gtttg                                                                                 >  1:6088940/1‑91 (MQ=255)
  tGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑Gtttg                                                                                 >  1:4350676/1‑91 (MQ=255)
   ggAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑Gtttgg                                                                                >  1:1532792/1‑90 (MQ=255)
   ggAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑Gtttgg                                                                                <  2:2132599/93‑4 (MQ=255)
   ggAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑Gtttgg                                                                                <  2:5823044/93‑4 (MQ=255)
   ggAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑Gtttgg                                                                                <  2:3772049/93‑4 (MQ=255)
    gAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑Gtttggt                                                                               <  2:4966347/93‑5 (MQ=255)
          aTGGCGGGCGGCGTTTCGAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACggg                                                                         <  1:8977973/93‑1 (MQ=255)
          aTGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACggg                                                                         >  1:750883/1‑93 (MQ=255)
          aTGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACggg                                                                         >  2:3448880/1‑93 (MQ=255)
           tGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGa                                                                        <  2:2881758/93‑1 (MQ=255)
           tGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGa                                                                        <  2:8409837/93‑1 (MQ=255)
           tGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGa                                                                        <  1:1803182/93‑1 (MQ=255)
           tGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGa                                                                        <  1:7474635/93‑1 (MQ=255)
            ggcgggcgGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGAt                                                                       <  1:3963700/93‑1 (MQ=255)
            ggcgggcgGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGAt                                                                       <  2:1818924/93‑1 (MQ=255)
             gcgggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATg                                                                      <  2:8626408/93‑1 (MQ=255)
             gcgggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATg                                                                      <  2:6051594/93‑1 (MQ=255)
               gggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGaa                                                                    <  1:9500316/93‑1 (MQ=255)
                ggcggcGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAac                                                                   <  2:7374816/93‑1 (MQ=255)
                ggcggcGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAac                                                                   >  1:8626918/1‑93 (MQ=255)
                ggcggcGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAac                                                                   <  1:10002085/93‑1 (MQ=255)
                ggcggcGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAac                                                                   >  1:5145751/1‑93 (MQ=255)
                ggcggcGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAac                                                                   <  2:4349308/93‑1 (MQ=255)
                 gcggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAacg                                                                  >  1:2907039/1‑93 (MQ=255)
                 gcggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGAGTGTATTGCGGTTG‑GTGACGGGATGAacg                                                                  >  1:6401998/1‑93 (MQ=255)
                  cggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAacga                                                                 <  1:10033062/93‑1 (MQ=255)
                  cggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAacga                                                                 <  2:641982/93‑1 (MQ=255)
                   ggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAacgac                                                                >  1:2739865/1‑93 (MQ=255)
                   ggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAacgac                                                                <  2:8753943/93‑1 (MQ=255)
                    gcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAacgacg                                                               >  2:5876638/1‑93 (MQ=255)
                    gcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAacgacg                                                               >  1:9759049/1‑93 (MQ=255)
                     cgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGc                                                              <  1:4552484/93‑1 (MQ=255)
                      gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGcc                                                             >  1:7202564/1‑93 (MQ=255)
                      gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGcc                                                             >  1:8630491/1‑93 (MQ=255)
                      gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGcc                                                             <  2:795387/93‑1 (MQ=255)
                      gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGcc                                                             <  1:6408410/93‑1 (MQ=255)
                      gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTNGGTGACGGGATGAACGACGcg                                                             >  1:8760513/1‑92 (MQ=255)
                        ttcaaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGa                                                           >  1:4004406/5‑93 (MQ=255)
                        ttcaaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGa                                                           >  2:3275038/5‑93 (MQ=255)
                        ttcaaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGa                                                           >  1:2638946/5‑93 (MQ=255)
                         tcaaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGaa                                                          >  2:5931017/4‑93 (MQ=255)
                         tcaaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGaa                                                          >  2:7064425/4‑93 (MQ=255)
                          caaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGaaa                                                         <  2:6578561/91‑1 (MQ=255)
                          caaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGaaa                                                         <  1:2353815/91‑1 (MQ=255)
                          caaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGaaa                                                         <  2:6411942/91‑1 (MQ=255)
                           aaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAAt                                                        >  1:865711/2‑93 (MQ=255)
                           aaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAAt                                                        >  2:8595104/2‑93 (MQ=255)
                           aaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAAt                                                        >  2:6351003/2‑93 (MQ=255)
                            aaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATg                                                       <  2:5419476/93‑1 (MQ=255)
                            aaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATg                                                       >  1:160295/1‑93 (MQ=255)
                             aaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGc                                                      <  1:2939580/93‑1 (MQ=255)
                              aGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGcg                                                     >  2:7669372/1‑92 (MQ=255)
                              aGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCt                                                     >  1:5588572/1‑93 (MQ=255)
                               ggCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTg                                                    <  2:7244493/93‑1 (MQ=255)
                               ggCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTg                                                    >  1:1345442/1‑93 (MQ=255)
                                                            actgggCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAAGGCTGCATTATggg                    <  1:8996608/89‑1 (MQ=255)
                                                            actgggCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAAGGCTGCATTATggg                    <  2:10047426/89‑1 (MQ=255)
                                                            actgggCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAAGGCTGCATTATggg                    >  1:7427623/5‑93 (MQ=255)
                                                            actgggCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAAGGCTGCATTATggg                    >  1:7644453/5‑93 (MQ=255)
                                                             ctgggCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGCAATGCTGTCGATGGCGGG‑G‑‑AAAGGCTGCATTATCGGc                   >  2:7442846/4‑93 (MQ=255)
                                                             ctgggCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAAGGCTGCATTATGGGc                   <  1:3790479/90‑1 (MQ=255)
                                                             ctgggCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAAGGCTGCATTATGGGc                   >  1:5283558/4‑93 (MQ=255)
                                                              tgggCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAAGGCTGCATTATGGGCa                  <  2:9225273/91‑1 (MQ=255)
                                                              tgggCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAAGGCTGCATTATGGGCa                  <  1:6431365/91‑1 (MQ=255)
                                                               gggCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAATGCTGCATTATGGGCAg                 <  2:9560886/92‑1 (MQ=255)
                                                                 gCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAAGGCTGCATTATGGGCAGTg               <  2:9885562/93‑1 (MQ=255)
                                                                 gCTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGAAGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAAGGCTGCATTATGGGCAGTg               <  1:6885365/93‑1 (MQ=255)
                                                                  cTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAAGGCTGCATTATGGGCAGTgc              >  1:7893199/1‑92 (MQ=255)
                                                                  cTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAAGGCTGCATTATGGGCAGTgc              <  1:6556074/93‑2 (MQ=255)
                                                                  cTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAAGGCTGCATTATGGGCAGTgc              >  2:7560954/1‑92 (MQ=255)
                                                                  cTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAAGGCTGCATTATGGGCAGTgc              >  2:3591843/1‑92 (MQ=255)
                                                                  cTACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAAGGCTGCATTATGGGCAGTgc              <  2:1340347/93‑2 (MQ=255)
                                                                   tACAGCCTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAAGGCTGCATTATGGGCAGTgcg             <  1:6646566/93‑3 (MQ=255)
                                                                       gccTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAAGGCTGCATTATGGGCAGTGCgcacc         <  2:9121825/91‑1 (MQ=255)
                                                                       gccTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAAGGCTGCATTATGGGCAGTGCgcacc         <  1:2179859/91‑1 (MQ=255)
                                                                        ccTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAAGGCTGCATTATGGGCAGTGCgcacca        >  2:2557917/2‑93 (MQ=255)
                                                                         cTGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAg       <  1:467010/93‑1 (MQ=255)
                                                                          tGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGc      >  2:2119997/1‑93 (MQ=255)
                                                                          tGAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGc      >  1:4394163/1‑93 (MQ=255)
                                                                           gAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAAGGTTGCATTATGGGCAGTGCGCACCAGCg     >  1:2268480/1‑93 (MQ=255)
                                                                           gAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCg     >  2:2587365/1‑93 (MQ=255)
                                                                           gAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCg     >  1:338086/1‑93 (MQ=255)
                                                                           gAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCg     >  2:6486777/1‑93 (MQ=255)
                                                                           gAAGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCg     <  2:4683520/93‑1 (MQ=255)
                                                                            aaGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGt    <  2:1114875/93‑1 (MQ=255)
                                                                            aaGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGt    <  1:3151164/93‑1 (MQ=255)
                                                                            aaGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGt    >  1:7039115/1‑93 (MQ=255)
                                                                            aaGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGt    <  2:5732570/93‑1 (MQ=255)
                                                                            aaGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGt    >  1:8016447/1‑93 (MQ=255)
                                                                             aGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTc   >  2:7801316/1‑93 (MQ=255)
                                                                             aGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTc   <  1:2424306/93‑1 (MQ=255)
                                                                             aGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTc   <  1:2050830/93‑1 (MQ=255)
                                                                             aGGATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGCG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTc   >  1:9231384/1‑93 (MQ=255)
                                                                              ggATTGTATTGC‑GTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATTGC‑GG‑GGCAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCt  >  2:4046463/1‑93 (MQ=255)
                                                                                    |                                                                                          
TCTGGAAGTGATGGCGGGCGGCGTTTC‑AAAGGCCATGCGCTGGAAG‑GCTGGCG‑‑‑‑AACT‑GGCTACAG‑CTGAAGGATTG‑ATTGC‑GTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑G‑‑AAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCT  >  NC_000913/4008346‑4008503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTCAAAGGCCATGCGCTGGAAG‑GCTGGCG‑‑‑‑AA‑‑‑‑CT‑GGCTACAG‑CTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑G‑‑AAA‑G‑CTGCATTATGGGCAGTGGCCACCAGCGTCTGAAA  >  NC_000913/4008370‑4008507
                                                              |                                                                                               
tcaAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAA                                                              <  SRR4302540.1699002/91‑1 (MQ=16)
 caAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAT                                                             <  SRR4302540.2379168/92‑1 (MQ=18)
 caAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAT                                                             >  SRR4302540.806546/3‑94 (MQ=18)
 caAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAT                                                             >  SRR4302540.874903/3‑94 (MQ=18)
 TAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAT                                                             <  SRR4302540.8843161/94‑1 (MQ=18)
  AAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATG                                                            >  SRR4302540.161911/1‑94 (MQ=19)
   AAAGGCCATGCGCTGGAAGCGGTGGCG‑‑‑‑AAGAATCTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGC                                                           <  SRR4302540.183462/94‑1 (MQ=21)
    AAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCT                                                          >  SRR4302540.5650079/1‑94 (MQ=21)
    AAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCT                                                          >  SRR4302540.5650078/1‑94 (MQ=21)
    AAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCT                                                          <  SRR4302540.2971856/94‑1 (MQ=21)
     AGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTG                                                         <  SRR4302540.2156594/94‑1 (MQ=23)
     AGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTG                                                         >  SRR4302540.7715878/1‑94 (MQ=23)
     AGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTG                                                         >  SRR4302540.8031009/1‑94 (MQ=23)
     AGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTG                                                         <  SRR4302540.10004879/94‑1 (MQ=23)
     AGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTG                                                         >  SRR4302540.1359571/1‑94 (MQ=23)
       GCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTC                                                       <  SRR4302540.3646314/94‑1 (MQ=26)
        CCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCG                                                      >  SRR4302540.4238846/1‑94 (MQ=28)
           gccatgctctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTC                                                       <  SRR4302540.7483348/62‑1 (MQ=38)
             catgcgctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGT                                                     >  SRR4302540.508408/31‑94 (MQ=40)
             catgcgctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGA                                                     >  SRR4302540.508409/31‑94 (MQ=42)
             catgcgctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGA                                                     <  SRR4302540.3364981/64‑1 (MQ=42)
             catgcgctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGA                                                     >  SRR4302540.5324835/31‑94 (MQ=42)
              atgcgctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGAT                                                    <  SRR4302540.1732967/65‑1 (MQ=45)
              atgcgctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGAT                                                    <  SRR4302540.9194727/65‑1 (MQ=45)
              atgcgctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGAT                                                    >  SRR4302540.8436599/30‑94 (MQ=45)
              atgcgctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGAT                                                    >  SRR4302540.9128906/30‑94 (MQ=45)
              atgcgctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGAT                                                    <  SRR4302540.4917563/65‑1 (MQ=45)
              atgcgctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGAT                                                    >  SRR4302540.7093685/30‑94 (MQ=45)
              atgcgctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGAT                                                    <  SRR4302540.9526765/65‑1 (MQ=45)
              atgcgctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGAT                                                    >  SRR4302540.7519695/30‑94 (MQ=45)
              atgcgctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGAT                                                    <  SRR4302540.4349373/65‑1 (MQ=45)
              atgcgctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTAGAT                                                    >  SRR4302540.2167333/30‑94 (MQ=34)
               tgcgctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATG                                                   <  SRR4302540.73176/66‑1 (MQ=47)
               tgcgctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATG                                                   <  SRR4302540.3355497/66‑1 (MQ=47)
                gcgctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG                                                  >  SRR4302540.332012/28‑94 (MQ=49)
                gcgctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG                                                  <  SRR4302540.8647840/67‑1 (MQ=49)
                 cgctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC                                                 >  SRR4302540.4068506/27‑94 (MQ=51)
                  gctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G                                               >  SRR4302540.3976907/26‑94 (MQ=53)
                  gctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G                                               >  SRR4302540.2070543/26‑94 (MQ=53)
                  gctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G                                               >  SRR4302540.1319173/26‑94 (MQ=53)
                  gctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G                                               >  SRR4302540.1319175/26‑94 (MQ=53)
                  gctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G                                               >  SRR4302540.3784977/26‑94 (MQ=53)
                  gctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G                                               <  SRR4302540.4006010/69‑1 (MQ=53)
                   ctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG                                              <  SRR4302540.3424547/70‑1 (MQ=55)
                   ctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG                                              >  SRR4302540.7373834/25‑94 (MQ=55)
                   ctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG                                              >  SRR4302540.5805393/25‑94 (MQ=55)
                   ctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG                                              <  SRR4302540.1626527/70‑1 (MQ=55)
                   ctggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGAAGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG                                              >  SRR4302540.5358055/25‑94 (MQ=41)
                    tggaagcggtggcgaagaaactgggctaccgcCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑G                                            <  SRR4302540.6812653/62‑1 (MQ=60)
                    tggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑G                                            >  SRR4302540.2788169/24‑94 (MQ=57)
                     ggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑G‑‑G                                         >  SRR4302540.5793723/23‑94 (MQ=56)
                     ggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑G‑‑G                                         <  SRR4302540.3583269/72‑1 (MQ=56)
                     ggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑G‑‑G                                         <  SRR4302540.4837762/72‑1 (MQ=56)
                     ggaagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑G‑‑G                                         >  SRR4302540.9361108/23‑94 (MQ=56)
                       aagcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑G‑‑GAA                                       >  SRR4302540.4642256/21‑94 (MQ=55)
                        agcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAA                                       <  SRR4302540.6008912/75‑1 (MQ=54)
                        agcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAA                                       <  SRR4302540.9853474/75‑1 (MQ=54)
                        agcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAA                                       >  SRR4302540.5979359/20‑94 (MQ=54)
                         gcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAA‑G                                     <  SRR4302540.9634746/76‑1 (MQ=53)
                         gcggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAA‑G                                     <  SRR4302540.9762757/76‑1 (MQ=53)
                         gcggtggcgaagaaacggGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑G‑‑AAA‑G                                     <  SRR4302540.4477921/76‑1 (MQ=53)
                           ggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑Ggaaaggc                                     <  SRR4302540.21553/78‑8 (MQ=57)
                           ggtggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑Ggaaaggc                                     <  SRR4302540.3329728/78‑8 (MQ=57)
                             tggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑Ggaaaggctg                                   <  SRR4302540.9967425/80‑10 (MQ=57)
                             tggcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑Ggaaaggctg                                   >  SRR4302540.9804660/15‑85 (MQ=57)
                               gcgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCA                               <  SRR4302540.5000314/82‑1 (MQ=50)
                                cgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCAT                              >  SRR4302540.6886699/12‑94 (MQ=49)
                                cgaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCAT                              >  SRR4302540.8501557/12‑94 (MQ=49)
                                 gaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATT                             >  SRR4302540.8099482/11‑94 (MQ=49)
                                 gaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATT                             >  SRR4302540.6413707/11‑94 (MQ=49)
                                 gaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATT                             >  SRR4302540.6413706/11‑94 (MQ=49)
                                 gaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATT                             >  SRR4302540.3392811/11‑94 (MQ=49)
                                 gaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATT                             >  SRR4302540.2835520/11‑94 (MQ=49)
                                 gaagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATT                             >  SRR4302540.9574949/11‑94 (MQ=49)
                                  aagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACTACGCCGAAATGCTGTCGATGGG‑GG‑GGCAAG‑G‑CTGCATTA                            >  SRR4302540.1963660/10‑94 (MQ=29)
                                  aagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑Ggaaaggctgcattt                              >  SRR4302540.3078795/10‑80 (MQ=57)
                                  aagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTA                            <  SRR4302540.2005748/85‑1 (MQ=48)
                                  aagaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTA                            <  SRR4302540.1162261/85‑1 (MQ=48)
                                  AA‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGG                        >  SRR4302540.7510367/1‑94 (MQ=50)
                                  AA‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGG                        >  SRR4302540.7730494/1‑94 (MQ=50)
                                  AA‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGG                        <  SRR4302540.6387578/94‑1 (MQ=50)
                                  AA‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGAGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGG                        <  SRR4302540.4674009/94‑1 (MQ=40)
                                   agaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTAT                           <  SRR4302540.5940236/86‑1 (MQ=48)
                                   agaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTAT                           <  SRR4302540.6763216/86‑1 (MQ=48)
                                   agaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTAT                           >  SRR4302540.1202810/9‑94 (MQ=48)
                                   agaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTAT                           <  SRR4302540.4982966/86‑1 (MQ=48)
                                   acaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTAT                           <  SRR4302540.4455428/86‑1 (MQ=48)
                                   A‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGC                       <  SRR4302540.9104530/94‑1 (MQ=53)
                                   A‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGC                       <  SRR4302540.7540608/94‑1 (MQ=53)
                                   A‑‑‑‑CTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGC                       >  SRR4302540.5341293/1‑94 (MQ=53)
                                    gaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATG                          >  SRR4302540.613597/8‑94 (MQ=49)
                                    gaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATG                          >  SRR4302540.3805053/8‑94 (MQ=49)
                                    gaaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATG                          <  SRR4302540.3106427/87‑1 (MQ=49)
                                     aaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGG                         <  SRR4302540.5883232/88‑1 (MQ=51)
                                     aaactgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGG                         >  SRR4302540.3177008/7‑94 (MQ=51)
                                      atctgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGG                        <  SRR4302540.9105269/89‑1 (MQ=53)
                                        ctgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCA                      <  SRR4302540.7427969/91‑1 (MQ=57)
                                        ctgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCA                      <  SRR4302540.1026428/91‑1 (MQ=57)
                                        ctgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCA                      <  SRR4302540.3831804/91‑1 (MQ=57)
                                         tgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAG                     <  SRR4302540.6502950/92‑1 (MQ=58)
                                         tgGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAG                     <  SRR4302540.3904400/92‑1 (MQ=58)
                                         G‑GGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGT                    <  SRR4302540.5643333/94‑1 (MQ=60)
                                            GCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGC                  >  SRR4302540.7985307/1‑94 (MQ=60)
                                            GCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGC                  <  SRR4302540.1506497/94‑1 (MQ=60)
                                            GCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGC                  <  SRR4302540.5148963/94‑1 (MQ=60)
                                            GCTACAGCCTGAAGGATTGTATTGCGTTTGGTGAAGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGC                  <  SRR4302540.6961699/94‑1 (MQ=48)
                                             CTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGcg                 >  SRR4302540.2311232/1‑92 (MQ=60)
                                             CTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGcg                 <  SRR4302540.6628771/94‑3 (MQ=60)
                                              TACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGcgc                >  SRR4302540.2354290/1‑91 (MQ=60)
                                              TACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGcgc                >  SRR4302540.4795597/1‑91 (MQ=60)
                                              TACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGcgc                <  SRR4302540.6720099/94‑4 (MQ=60)
                                                CAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGcgcac              >  SRR4302540.1519608/1‑89 (MQ=60)
                                                CAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGcgcac              <  SRR4302540.9255566/94‑6 (MQ=60)
                                                CAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGcgcac              >  SRR4302540.872566/1‑89 (MQ=60)
                                                CAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGcgcac              <  SRR4302540.959455/94‑6 (MQ=60)
                                                CAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGcgcac              >  SRR4302540.1811185/1‑89 (MQ=60)
                                                CAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGcgcac              <  SRR4302540.2741885/94‑6 (MQ=60)
                                                  gcCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCA            <  SRR4302540.2203366/92‑1 (MQ=60)
                                                  C‑CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAG           <  SRR4302540.4701207/94‑1 (MQ=60)
                                                    CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGC          <  SRR4302540.472006/94‑1 (MQ=60)
                                                    CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGC          >  SRR4302540.4472460/1‑94 (MQ=60)
                                                    CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGC          >  SRR4302540.4441843/1‑94 (MQ=60)
                                                    CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGC          >  SRR4302540.4441842/1‑94 (MQ=60)
                                                    CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGC          <  SRR4302540.3419595/94‑1 (MQ=60)
                                                    CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGC          >  SRR4302540.6890283/1‑94 (MQ=60)
                                                    CTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGC          <  SRR4302540.9093719/94‑1 (MQ=60)
                                                     TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑TTGCATTATGGGCAGTGCGCACCAGCG         >  SRR4302540.2292819/1‑94 (MQ=46)
                                                     TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGcgtaccagcg         <  SRR4302540.795670/94‑11 (MQ=60)
                                                     TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCG         >  SRR4302540.341493/1‑94 (MQ=57)
                                                      GAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGT        >  SRR4302540.8109688/1‑94 (MQ=57)
                                                      GAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGT        >  SRR4302540.7117369/1‑94 (MQ=57)
                                                       AAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGCG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTC       >  SRR4302540.9342030/1‑94 (MQ=55)
                                                       AAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTC       <  SRR4302540.1554490/94‑1 (MQ=57)
                                                       AAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTC       >  SRR4302540.768188/1‑94 (MQ=57)
                                                       AAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTC       <  SRR4302540.3185476/94‑1 (MQ=57)
                                                       AAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTC       <  SRR4302540.2560071/94‑1 (MQ=57)
                                                        AGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCT      <  SRR4302540.2072910/94‑1 (MQ=57)
                                                        AGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCT      <  SRR4302540.2450424/94‑1 (MQ=57)
                                                        AGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCT      >  SRR4302540.7281347/1‑94 (MQ=57)
                                                        AGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCT      <  SRR4302540.2274392/94‑1 (MQ=57)
                                                         GGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTG     >  SRR4302540.9269028/1‑94 (MQ=60)
                                                          GATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGCTGGCGGG‑G‑‑AAA‑GCCTGCATTATGGGCAGTGCGCACCAGCGTCTGA    >  SRR4302540.9003392/1‑94 (MQ=48)
                                                           ATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAA   >  SRR4302540.5840618/1‑94 (MQ=60)
                                                            TTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAG  >  SRR4302540.8345978/1‑94 (MQ=60)
                                                            TTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAA  >  SRR4302540.8449313/1‑94 (MQ=60)
                                                            TTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAA  >  SRR4302540.1070290/1‑94 (MQ=60)
                                                              |                                                                                               
TTCAAAGGCCATGCGCTGGAAG‑GCTGGCG‑‑‑‑AA‑‑‑‑CT‑GGCTACAG‑CTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑G‑‑AAA‑G‑CTGCATTATGGGCAGTGGCCACCAGCGTCTGAAA  >  NC_000913/4008370‑4008507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑