Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	641,051	T→C	M14V (ATG→GTG)	ybdQ ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	641,051	0	T	C	100.0%	779.8 / NA	236	M14V (ATG→GTG)	ybdQ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (79/157); total (79/157)

Only 100 of 238 total aligned reads displayed.

CTGACCCGGGTAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCATTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATGATTATAAT  >  NC_000913/640961‑641140

ctgACCCGGGTAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCActt                                                                                         <  1:542521/93‑1 (MQ=255)

ctgACCCGGGTAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCActt                                                                                         <  2:3427797/93‑1 (MQ=255)

 tgACCCGGGTAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTc                                                                                        <  2:481160/93‑1 (MQ=255)

  gACCCGGGTAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCa                                                                                       <  1:5692424/93‑1 (MQ=255)

  gACCCGGGTAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCa                                                                                       <  1:918155/93‑1 (MQ=255)

  gACCCGGGTAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCa                                                                                       >  1:2233462/1‑93 (MQ=255)

   aCCCGGGTAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCaa                                                                                      <  1:987686/93‑1 (MQ=255)

     ccGGGTAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAAt                                                                                    >  1:6184369/1‑93 (MQ=255)

        ggTAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACa                                                                                 >  1:4041797/1‑93 (MQ=255)

        ggTAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACa                                                                                 <  1:2095055/93‑1 (MQ=255)

         gtagtaCGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACAt                                                                                <  1:5751575/93‑1 (MQ=255)

          tagtaCGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATc                                                                               <  2:3904776/93‑1 (MQ=255)

           agtaCGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCa                                                                              >  1:5128590/1‑93 (MQ=255)

           agtaCGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCa                                                                              >  2:2539278/1‑93 (MQ=255)

            gtaCGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCaa                                                                             <  1:3860917/93‑1 (MQ=255)

            gtaCGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCaa                                                                             >  1:5601096/1‑93 (MQ=255)

             taCGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAAc                                                                            >  2:5798426/1‑93 (MQ=255)

              aCGTGAAGTAGATGAATACCTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACt                                                                           <  1:3232836/93‑1 (MQ=255)

                gTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTgg                                                                         <  1:1589535/93‑1 (MQ=255)

                 tGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTGGCTCAATTCCACTTCAAATACATCAACTGGc                                                                        <  2:1880263/93‑1 (MQ=255)

                 tGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGc                                                                        >  1:136260/1‑93 (MQ=255)

                  gAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCa                                                                       >  1:731925/1‑93 (MQ=255)

                  gAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCa                                                                       <  2:2338163/93‑1 (MQ=255)

                  gAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGATCAATTCCACTTCAAATACATCAACTGGCa                                                                       <  1:6303284/93‑1 (MQ=255)

                    aGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATa                                                                     <  1:5823699/93‑1 (MQ=255)

                      tAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAAt                                                                   <  2:3550879/93‑1 (MQ=255)

                        gATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGa                                                                 <  1:1916131/93‑1 (MQ=255)

                        gATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGa                                                                 <  2:4638490/93‑1 (MQ=255)

                        gATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTCTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGa                                                                 <  1:4442847/93‑1 (MQ=255)

                         aTGAATAGCTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGAt                                                                >  2:5309688/1‑93 (MQ=255)

                         aTGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGAt                                                                <  1:770717/93‑1 (MQ=255)

                          tGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGAtt                                                               <  2:5156522/93‑1 (MQ=255)

                           gAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTg                                                              <  2:4897546/93‑1 (MQ=255)

                           gAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTg                                                              <  1:6101951/93‑1 (MQ=255)

                             aTAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTc                                                            <  2:5364369/93‑1 (MQ=255)

                             aTAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTc                                                            >  2:4039604/1‑93 (MQ=255)

                              tAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCt                                                           <  2:286856/93‑1 (MQ=255)

                                aCTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTta                                                         <  1:2795597/93‑1 (MQ=255)

                                  tCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTtata                                                       >  1:2217700/1‑93 (MQ=255)

                                  tCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTtata                                                       >  1:3691182/1‑93 (MQ=255)

                                  tCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTtata                                                       <  2:1707741/93‑1 (MQ=255)

                                  tCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTtata                                                       <  1:2643593/93‑1 (MQ=255)

                                  tCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTtata                                                       <  2:1840490/93‑1 (MQ=255)

                                  tCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTtata                                                       >  1:55841/1‑93 (MQ=255)

                                   ccGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATAc                                                      <  2:2919549/93‑1 (MQ=255)

                                   ccGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATAc                                                      >  1:2511605/1‑93 (MQ=255)

                                    cGTCATCCTGGGCGAGGAATTCAGCGTGTCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACa                                                     <  2:3734422/93‑1 (MQ=255)

                                    cGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACa                                                     >  2:493326/1‑93 (MQ=255)

                                    cGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACa                                                     <  2:5490311/93‑1 (MQ=255)

                                    cGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACa                                                     >  1:1983882/1‑93 (MQ=255)

                                     gTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACAt                                                    >  2:5268234/1‑93 (MQ=255)

                                      tCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATa                                                   >  2:4531308/1‑93 (MQ=255)

                                      tCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATa                                                   <  2:2436454/93‑1 (MQ=255)

                                       cATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATaa                                                  <  2:1258800/93‑1 (MQ=255)

                                        aTCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAAc                                                 >  1:4362599/1‑93 (MQ=255)

                                         tCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAAcc                                                >  2:4138465/1‑93 (MQ=255)

                                         tCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAAcc                                                >  1:1721766/1‑93 (MQ=255)

                                          ccTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAAccc                                               <  2:149217/93‑1 (MQ=255)

                                              ggCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAGCCCTTtc                                           <  2:1949908/93‑1 (MQ=255)

                                               gCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTtct                                          <  1:257471/93‑1 (MQ=255)

                                                  aGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTccc                                       <  2:2314552/93‑1 (MQ=255)

                                                   ggAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCt                                      <  1:5503485/93‑1 (MQ=255)

                                                   ggAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGCCTTATACATAACCCTTTCTCCCt                                      >  1:4186977/1‑93 (MQ=255)

                                                     aagtCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATAGTCTTATACATAACCCTTTCCCCCTGt                                    <  1:1550504/90‑1 (MQ=255)

                                                           gCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCa                              >  2:3166943/1‑93 (MQ=255)

                                                            cGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCAt                             <  1:193664/93‑1 (MQ=255)

                                                             gTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATg                            <  2:686223/93‑1 (MQ=255)

                                                                gCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATACCCCTTTCTCCCTGTTAATCATGAAc                         <  1:1435932/93‑1 (MQ=255)

                                                                gCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAAc                         <  1:2062253/93‑1 (MQ=255)

                                                                 cGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACa                        <  1:3678876/93‑1 (MQ=255)

                                                                 cGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACa                        <  1:4123428/93‑1 (MQ=255)

                                                                 cGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACa                        <  2:3610954/93‑1 (MQ=255)

                                                                 cGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACa                        >  2:5747728/1‑93 (MQ=255)

                                                                  gAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACaa                       <  2:5226352/93‑1 (MQ=255)

                                                                   aaCAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAATCCTTTCTCCCTGTTAATCATGAACaaa                      >  1:3070458/1‑93 (MQ=255)

                                                                   aaCAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACaaa                      <  1:3784686/93‑1 (MQ=255)

                                                                    aCAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAAt                     <  1:595887/93‑1 (MQ=255)

                                                                    aCAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAAt                     <  1:2220407/93‑1 (MQ=255)

                                                                     cAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATc                    <  1:3592201/93‑1 (MQ=255)

                                                                      aGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCa                   <  1:3371782/93‑1 (MQ=255)

                                                                        cTTTGTCGCTCAATTCCACTTCAAATGCATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCAtt                 <  2:6506/93‑1 (MQ=255)

                                                                        cTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCAtt                 <  2:238465/93‑1 (MQ=255)

                                                                        cTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCAtt                 <  2:5005572/93‑1 (MQ=255)

                                                                         tttGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTc                >  2:832056/1‑93 (MQ=255)

                                                                         tttGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTc                <  1:5850798/93‑1 (MQ=255)

                                                                            gTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGcc             <  2:4412886/93‑1 (MQ=255)

                                                                             tCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCa            <  1:107907/93‑1 (MQ=255)

                                                                              cGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCAt           <  1:3089357/93‑1 (MQ=255)

                                                                              cGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCAt           <  1:5322641/93‑1 (MQ=255)

                                                                               gCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATg          >  1:4302547/1‑93 (MQ=255)

                                                                               gCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATg          <  2:419203/93‑1 (MQ=255)

                                                                                 tCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATGAt        >  2:1154524/1‑93 (MQ=255)

                                                                                  cAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATGAtt       <  2:3144570/93‑1 (MQ=255)

                                                                                   aaTTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATGATta      >  1:2939941/1‑93 (MQ=255)

                                                                                    aTTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATGATtat     <  1:4633868/93‑1 (MQ=255)

                                                                                    aTTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATGATtat     <  1:4898072/93‑1 (MQ=255)

                                                                                    aTTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATGATtat     <  1:494462/93‑1 (MQ=255)

                                                                                    aTTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATGATtat     <  2:5989197/93‑1 (MQ=255)

                                                                                     ttCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATGATtata    <  2:5440974/93‑1 (MQ=255)

                                                                                       ccACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATGATTataat  >  2:6054358/1‑93 (MQ=255)

CTGACCCGGGTAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCATTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATGATTATAAT  >  NC_000913/640961‑641140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGGCTGACCCGGGTAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCATTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATGATTATAATATTT  >  NC_000913/640958‑641144

TGGCTGACCCGGGTAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCAC                                                                                               <  SRR4302533.1809438/94‑1 (MQ=60)

 GGCTGACCCGGGTAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACT                                                                                              <  SRR4302533.6178692/94‑1 (MQ=60)

 GGCTGACCCGGGTAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACT                                                                                              <  SRR4302533.3356599/94‑1 (MQ=60)

 GGCTGACCCGGGTAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACT                                                                                              <  SRR4302533.3479768/94‑1 (MQ=60)

   CTGACCCGGGTAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTC                                                                                            <  SRR4302533.545742/94‑1 (MQ=60)

    TGACCCGGGTAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCA                                                                                           >  SRR4302533.2247245/1‑94 (MQ=60)

    TGACCCGGGTAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCA                                                                                           >  SRR4302533.322786/1‑94 (MQ=60)

    TGACCCGGGTAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCA                                                                                           >  SRR4302533.5007266/1‑94 (MQ=60)

     GACCCGGGTAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAA                                                                                          <  SRR4302533.923549/94‑1 (MQ=60)

     GACCCGGGTAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAA                                                                                          <  SRR4302533.5726803/94‑1 (MQ=60)

     GACCCGGGTAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAA                                                                                          <  SRR4302533.3367596/94‑1 (MQ=60)

      ACCCGGGTAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAA                                                                                         <  SRR4302533.993465/94‑1 (MQ=60)

      ACCCGGGTAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAA                                                                                         <  SRR4302533.3100590/94‑1 (MQ=60)

       CCCGGGTAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAAT                                                                                        <  SRR4302533.5816306/94‑1 (MQ=60)

       CCCGGGTAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAAT                                                                                        >  SRR4302533.6221500/1‑94 (MQ=60)

       CACGGGTAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAAT                                                                                        <  SRR4302533.431817/94‑1 (MQ=60)

        CCGGGTAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATA                                                                                       >  SRR4302533.5728373/1‑94 (MQ=60)

         CGGGTAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATAC                                                                                      >  SRR4302533.4866037/1‑94 (MQ=60)

         CGGGTAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATAC                                                                                      >  SRR4302533.2581464/1‑94 (MQ=60)

          GGGTAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACA                                                                                     >  SRR4302533.4066396/1‑94 (MQ=60)

          GGGTAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACA                                                                                     >  SRR4302533.1321891/1‑94 (MQ=60)

           GGTAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACAT                                                                                    <  SRR4302533.2107955/94‑1 (MQ=60)

           GGTAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACAT                                                                                    <  SRR4302533.3719383/94‑1 (MQ=60)

            GTAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATC                                                                                   <  SRR4302533.5786274/94‑1 (MQ=60)

             TAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCA                                                                                  >  SRR4302533.5159572/1‑94 (MQ=60)

              AGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAA                                                                                 >  SRR4302533.4350928/1‑94 (MQ=60)

              AGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAA                                                                                 >  SRR4302533.5634943/1‑94 (MQ=60)

               GTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAAC                                                                                >  SRR4302533.2009178/1‑94 (MQ=60)

               GTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAAC                                                                                >  SRR4302533.5535493/1‑94 (MQ=60)

               GTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAAC                                                                                <  SRR4302533.3884485/94‑1 (MQ=60)

                TACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACT                                                                               <  SRR4302533.5627867/94‑1 (MQ=60)

                 ACGTGAAGTAGATGAATACCTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTG                                                                              <  SRR4302533.3252756/94‑1 (MQ=60)

                 ACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTG                                                                              <  SRR4302533.2510991/94‑1 (MQ=60)

                   GTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGC                                                                            <  SRR4302533.1599147/94‑1 (MQ=60)

                   GTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGC                                                                            >  SRR4302533.137120/1‑94 (MQ=60)

                    TGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCA                                                                           >  SRR4302533.736233/1‑94 (MQ=60)

                     GAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGATCAATTCCACTTCAAATACATCAACTGGCAT                                                                          <  SRR4302533.6340957/94‑1 (MQ=60)

                      AAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATA                                                                         <  SRR4302533.5649723/94‑1 (MQ=60)

                       AGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAA                                                                        <  SRR4302533.5858876/94‑1 (MQ=60)

                       AGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAA                                                                        <  SRR4302533.2616111/94‑1 (MQ=60)

                       AGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAA                                                                        <  SRR4302533.1499068/94‑1 (MQ=60)

                        GTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAAT                                                                       >  SRR4302533.95453/1‑94 (MQ=60)

                        GTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAAT                                                                       <  SRR4302533.1573488/94‑1 (MQ=60)

                         TAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATG                                                                      >  SRR4302533.2085722/1‑94 (MQ=60)

                          AGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGA                                                                     <  SRR4302533.2440619/94‑1 (MQ=60)

                          AGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGA                                                                     >  SRR4302533.4151374/1‑94 (MQ=60)

                           GATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGAT                                                                    <  SRR4302533.1927829/94‑1 (MQ=60)

                           GATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTCTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGAT                                                                    <  SRR4302533.4469716/94‑1 (MQ=60)

                            ATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATT                                                                   <  SRR4302533.775254/94‑1 (MQ=60)

                             TGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTG                                                                  >  SRR4302533.3650667/1‑94 (MQ=60)

                              GAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGT                                                                 <  SRR4302533.6138791/94‑1 (MQ=60)

                              GAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGT                                                                 <  SRR4302533.1970215/94‑1 (MQ=60)

                              GAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGT                                                                 <  SRR4302533.3010017/94‑1 (MQ=60)

                                 TAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTT                                                              <  SRR4302533.4409107/94‑1 (MQ=60)

                                  AACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTA                                                             >  SRR4302533.2233244/1‑94 (MQ=60)

                                   ACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTAT                                                            <  SRR4302533.3882625/94‑1 (MQ=60)

                                   ACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTAT                                                            >  SRR4302533.5996962/1‑94 (MQ=60)

                                   ACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTAT                                                            <  SRR4302533.1389970/94‑1 (MQ=60)

                                   ACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTAT                                                            <  SRR4302533.2812790/94‑1 (MQ=60)

                                    CTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATA                                                           >  SRR4302533.2231390/1‑94 (MQ=60)

                                    CTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATA                                                           >  SRR4302533.56215/1‑94 (MQ=60)

                                    CTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATA                                                           >  SRR4302533.3696776/1‑94 (MQ=60)

                                    CTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATA                                                           >  SRR4302533.3713861/1‑94 (MQ=60)

                                     TCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATAC                                                          >  SRR4302533.2527081/1‑94 (MQ=60)

                                     TCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATAC                                                          <  SRR4302533.2659837/94‑1 (MQ=60)

                                      CCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACA                                                         >  SRR4302533.1996022/1‑94 (MQ=60)

                                       CGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACAT                                                        <  SRR4302533.5999718/94‑1 (MQ=60)

                                        GTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATA                                                       <  SRR4302533.3050499/94‑1 (MQ=60)

                                          CATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAAC                                                     >  SRR4302533.4388931/1‑94 (MQ=60)

                                          CATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAAC                                                     <  SRR4302533.1673329/94‑1 (MQ=60)

                                           ATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACC                                                    >  SRR4302533.68243/1‑94 (MQ=60)

                                           ATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACC                                                    <  SRR4302533.843963/94‑1 (MQ=60)

                                           ATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACC                                                    >  SRR4302533.1732207/1‑94 (MQ=60)

                                             CCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCT                                                  <  SRR4302533.4597885/94‑1 (MQ=60)

                                             CCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCT                                                  <  SRR4302533.2238682/94‑1 (MQ=60)

                                                GGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTC                                               <  SRR4302533.6141182/94‑1 (MQ=60)

                                                GGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCGCAATGCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTC                                               <  SRR4302533.4081182/94‑1 (MQ=60)

                                                  GCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTC                                             <  SRR4302533.1821779/94‑1 (MQ=60)

                                                  GCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTC                                             <  SRR4302533.259037/94‑1 (MQ=60)

                                                   CGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCC                                            >  SRR4302533.526105/1‑94 (MQ=60)

                                                     AGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGCCTTATACATAACCCTTTCTCCCT                                          >  SRR4302533.4212331/1‑94 (MQ=60)

                                                      GGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTG                                         <  SRR4302533.5536689/94‑1 (MQ=60)

                                                      GGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTG                                         <  SRR4302533.5845226/94‑1 (MQ=60)

                                                        AAGTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATAGTCTTATACATAACCCTTTCCCCCTGTT                                       <  SRR4302533.1559846/94‑1 (MQ=60)

                                                         ATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTA                                      <  SRR4302533.3841102/94‑1 (MQ=60)

                                                           TCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAAT                                    <  SRR4302533.3708205/94‑1 (MQ=60)

                                                           TCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAAT                                    <  SRR4302533.4374947/94‑1 (MQ=60)

                                                           TCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAAT                                    <  SRR4302533.2947241/94‑1 (MQ=60)

                                                             AGCGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCA                                  >  SRR4302533.2760065/1‑94 (MQ=60)

                                                               CGTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATG                                <  SRR4302533.194886/94‑1 (MQ=60)

                                                                GTGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGA                               >  SRR4302533.4006726/1‑94 (MQ=60)

                                                                 TGGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAA                              >  SRR4302533.4891583/1‑94 (MQ=60)

                                                                  GGCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAAC                             <  SRR4302533.2603052/94‑1 (MQ=60)

                                                                   GCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATACCCCTTTCTCCCTGTTAATCATGAACA                            <  SRR4302533.1444600/94‑1 (MQ=60)

                                                                   GCGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACA                            <  SRR4302533.2074970/94‑1 (MQ=60)

                                                                    CGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAA                           <  SRR4302533.4148463/94‑1 (MQ=60)

                                                                    CGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAA                           <  SRR4302533.3701490/94‑1 (MQ=60)

                                                                    CGAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCAGAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAA                           >  SRR4302533.6226086/1‑94 (MQ=60)

                                                                     GAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAATCCTTTCTCCCTGTTAATCATGAACAAA                          >  SRR4302533.3089311/1‑94 (MQ=60)

                                                                     GAACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAA                          >  SRR4302533.4988875/1‑94 (MQ=60)

                                                                      AACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAAT                         <  SRR4302533.5397052/94‑1 (MQ=60)

                                                                      AACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAAT                         >  SRR4302533.33653/1‑94 (MQ=60)

                                                                      AACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAAT                         <  SRR4302533.3807860/94‑1 (MQ=60)

                                                                       ACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATC                        <  SRR4302533.2234114/94‑1 (MQ=60)

                                                                       ACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATC                        <  SRR4302533.6291300/94‑1 (MQ=60)

                                                                       ACAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATC                        <  SRR4302533.599401/94‑1 (MQ=60)

                                                                        CAGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCA                       <  SRR4302533.3614311/94‑1 (MQ=60)

                                                                         AGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCAT                      <  SRR4302533.1939748/94‑1 (MQ=60)

                                                                         AGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCAT                      <  SRR4302533.3392602/94‑1 (MQ=60)

                                                                         AGCTTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCAT                      <  SRR4302533.5860333/94‑1 (MQ=60)

                                                                            TTTGTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCG                   <  SRR4302533.5886171/94‑1 (MQ=60)

                                                                               GTCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCA                <  SRR4302533.1973577/94‑1 (MQ=60)

                                                                                TCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCAT               <  SRR4302533.108586/94‑1 (MQ=60)

                                                                                TCGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCAT               <  SRR4302533.4947596/94‑1 (MQ=60)

                                                                                 CGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATG              >  SRR4302533.4124718/1‑94 (MQ=60)

                                                                                 CGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATG              >  SRR4302533.748831/1‑94 (MQ=60)

                                                                                 CGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATG              <  SRR4302533.5354539/94‑1 (MQ=60)

                                                                                 CGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATG              <  SRR4302533.3108347/94‑1 (MQ=60)

                                                                                 CGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATG              <  SRR4302533.1455243/94‑1 (MQ=60)

                                                                                 CGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATG              <  SRR4302533.1833502/94‑1 (MQ=60)

                                                                                 CGCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATG              >  SRR4302533.4328431/1‑94 (MQ=60)

                                                                                  GCTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATGA             >  SRR4302533.2131857/1‑94 (MQ=60)

                                                                                   CTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATGAT            >  SRR4302533.5703766/1‑94 (MQ=60)

                                                                                   CTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATGAT            <  SRR4302533.2133843/94‑1 (MQ=60)

                                                                                   CTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATGAT            <  SRR4302533.797709/94‑1 (MQ=60)

                                                                                   CTCAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATGAT            <  SRR4302533.5491830/94‑1 (MQ=60)

                                                                                     CAATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATGATTA          >  SRR4302533.2957900/1‑94 (MQ=60)

                                                                                      AATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATGATTAT         <  SRR4302533.5610281/94‑1 (MQ=60)

                                                                                      AATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATGATTAT         >  SRR4302533.5942895/1‑94 (MQ=60)

                                                                                       ATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATGATTATA        <  SRR4302533.4927752/94‑1 (MQ=60)

                                                                                       ATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATGATTATA        <  SRR4302533.497411/94‑1 (MQ=60)

                                                                                       ATTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATGATTATA        <  SRR4302533.4661947/94‑1 (MQ=60)

                                                                                        TTCCACTTCAAATACATCAACTGGCATAATGATTGTCTTATACGTAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATGATTATAA       >  SRR4302533.3407326/1‑94 (MQ=60)

                                                                                           CACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATGATTATAATAT    >  SRR4302533.4836196/1‑94 (MQ=60)

                                                                                            ACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATGATTATAATATT   <  SRR4302533.3097614/94‑1 (MQ=60)

                                                                                            ACTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATGATTATAATATT   >  SRR4302533.5015485/1‑94 (MQ=60)

                                                                                             CTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATGATTATAATATTT  <  SRR4302533.6187691/94‑1 (MQ=60)

                                                                                             CTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATGATTATAATATTT  <  SRR4302533.3308833/94‑1 (MQ=60)

                                                                                             CTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATGATTATAATATTT  <  SRR4302533.21260/94‑1 (MQ=60)

TGGCTGACCCGGGTAGTACGTGAAGTAGATGAATAACTCCGTCATCCTGGGCGAGGAATTCAGCGTGGCGAACAGCTTTGTCGCTCAATTCCATTTCAAATACATCAACTGGCATAATGATTGTCTTATACATAACCCTTTCTCCCTGTTAATCATGAACAAATCATTCGCCATGATTATAATATTT  >  NC_000913/640958‑641144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`