Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 669,057 (G)6→7 coding (74/612 nt) phpB ← CobC‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913669,0511.G100.0% 565.1 / NA 175L27P (CTG→CCG) phpBCobC‑like protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (96/79);  total (96/79)

Only 100 of 215 total aligned reads displayed.

CCCGTTCCAGTTCACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CA‑GGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCC  >  NC_000913/668959‑669136
                                                                                              |                                                                                     
cccGTTCCAGTTCACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑Ca                                                                                        >  2:3384050/1‑93 (MQ=255)
 ccGTTCCAGTTCACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CA‑g                                                                                      >  2:3072817/1‑93 (MQ=255)
  cGTTCCAGTTCACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CA‑gg                                                                                     <  2:4554599/93‑1 (MQ=255)
  cGTTCCAGTTCACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CA‑gg                                                                                     <  1:2462589/93‑1 (MQ=255)
   gTTCCAGTTCACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CA‑ggg                                                                                    <  1:4045140/93‑1 (MQ=255)
    ttCCAGTTCACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CA‑gggg                                                                                   <  1:986605/93‑1 (MQ=255)
     tCCAGTTCACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CA‑ggggg                                                                                  <  2:360306/93‑1 (MQ=255)
      ccAGTTCACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CA‑gggggg                                                                                 <  2:6260324/93‑1 (MQ=255)
      ccAGTTCACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CA‑gggggg                                                                                 <  2:3122243/93‑1 (MQ=255)
       cAGTTCACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CA‑ggggggg                                                                                >  2:3723703/1‑92 (MQ=255)
       cAGTTAACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCCCTTGCTCAATACCGCGCCCGGT‑CA‑ggggggg                                                                                <  2:6089543/93‑2 (MQ=255)
        aGTTCACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CA‑gggggggt                                                                               <  1:5863000/93‑3 (MQ=255)
         gTTCACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CA‑gggggggtg                                                                              >  1:5617084/1‑90 (MQ=255)
              cTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGgcgc                                                                          >  1:4659475/1‑93 (MQ=255)
              cTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGgcgc                                                                          >  1:6287122/1‑93 (MQ=255)
              cTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGgcgc                                                                          >  1:3780877/1‑93 (MQ=255)
               tGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCa                                                                         >  1:4504392/1‑93 (MQ=255)
               tGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCa                                                                         <  2:2350893/93‑1 (MQ=255)
               tGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCa                                                                         <  2:167062/93‑1 (MQ=255)
                  aTAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGa                                                                      <  2:3895580/93‑1 (MQ=255)
                  aTAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGa                                                                      <  1:1072309/93‑1 (MQ=255)
                   tAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGAc                                                                     >  2:891501/1‑93 (MQ=255)
                   tAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGAGCATGAc                                                                     >  1:5268319/1‑93 (MQ=255)
                     aaaCCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCg                                                                   >  1:5489664/1‑93 (MQ=255)
                      aaCCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGc                                                                  <  1:760385/93‑1 (MQ=255)
                      aaCCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGc                                                                  <  1:5665564/93‑1 (MQ=255)
                      aaCCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGc                                                                  <  2:5256853/93‑1 (MQ=255)
                       aCCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGCGGGCGCATGACCGCt                                                                 >  1:4849192/1‑93 (MQ=255)
                        ccAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTg                                                                <  1:934255/93‑1 (MQ=255)
                        ccAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTg                                                                >  1:239041/1‑93 (MQ=255)
                            aTCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTaaa                                                            >  1:2118022/1‑93 (MQ=255)
                             tCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAg                                                           >  1:657386/1‑93 (MQ=255)
                               aaaGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGAc                                                         >  1:3449056/1‑93 (MQ=255)
                               aaaGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGAc                                                         >  2:2526876/1‑93 (MQ=255)
                               aaaGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGAc                                                         >  2:2864052/1‑93 (MQ=255)
                               aaaGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGAc                                                         >  2:3019130/1‑93 (MQ=255)
                               aaaGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGAc                                                         >  2:4069590/1‑93 (MQ=255)
                               aaaGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGAc                                                         >  1:3415254/1‑93 (MQ=255)
                                aaGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGAcc                                                        >  1:3810016/1‑93 (MQ=255)
                                aaGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGAcc                                                        <  1:2947089/93‑1 (MQ=255)
                                 aGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCa                                                       >  2:4638771/1‑93 (MQ=255)
                                    aaaCACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCg                                                    >  1:3930749/1‑93 (MQ=255)
                                      acacCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGat                                                  >  2:681134/1‑93 (MQ=255)
                                      acacCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGat                                                  <  2:2557981/93‑1 (MQ=255)
                                        acCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGatat                                                >  1:5576259/1‑93 (MQ=255)
                                        acCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGCGGGCGCATGACCGCTGTAAAGAACATCGatat                                                >  2:1504193/1‑93 (MQ=255)
                                        acCATGTAGCAGCGTATGCAGATCTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGatat                                                <  2:1968008/93‑1 (MQ=255)
                                         ccATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATAtt                                               >  2:5402785/1‑93 (MQ=255)
                                          cATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTc                                              <  1:1393162/93‑1 (MQ=255)
                                          cATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTc                                              <  2:2213476/93‑1 (MQ=255)
                                          cATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGGGGGCGCATGACCGCTGTAAAGAACCTCGATATTc                                              >  2:1191061/1‑93 (MQ=255)
                                            tGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGc                                            >  1:4320744/1‑93 (MQ=255)
                                            tGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGc                                            >  1:5112214/1‑93 (MQ=255)
                                            tGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGc                                            <  1:2252616/93‑1 (MQ=255)
                                             gTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCt                                           <  2:4340505/93‑1 (MQ=255)
                                             gTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCt                                           <  2:1567864/93‑1 (MQ=255)
                                              tAGCAGCGTATGCAGATTTTGCGCTTGCTCGATACCGCGGGTGGTGGG‑GGGGGGTGGGGGGGGGACCGCTGTAAAGACCATCGATATTCGCtt                                          <  2:2576885/93‑1 (MQ=255)
                                              tAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCtt                                          <  2:3669474/93‑1 (MQ=255)
                                              tAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCtt                                          <  1:3965481/93‑1 (MQ=255)
                                                gcagcGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGc                                        <  2:5352251/93‑1 (MQ=255)
                                                   gcGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGtt                                     >  1:5071980/1‑93 (MQ=255)
                                                   gcGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGtt                                     >  1:3199665/1‑93 (MQ=255)
                                                     gTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTc                                   >  2:5789167/1‑93 (MQ=255)
                                                     gTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTc                                   <  1:2727196/93‑1 (MQ=255)
                                                      tATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCa                                  >  2:4501143/1‑93 (MQ=255)
                                                        tGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCAcc                                <  2:5033019/93‑1 (MQ=255)
                                                        tGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCAcc                                >  1:104726/1‑93 (MQ=255)
                                                        tGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCAcc                                <  1:2123477/93‑1 (MQ=255)
                                                         gCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCa                               <  2:2131962/93‑1 (MQ=255)
                                                         gCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCa                               <  2:4937401/93‑1 (MQ=255)
                                                         gCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCa                               >  2:3003548/1‑93 (MQ=255)
                                                         gCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCa                               >  2:2652268/1‑93 (MQ=255)
                                                         gCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCa                               >  2:5646695/1‑93 (MQ=255)
                                                            gATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGa                            <  1:3604714/93‑1 (MQ=255)
                                                            gATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGa                            <  1:4370719/93‑1 (MQ=255)
                                                            gATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGa                            <  2:1214245/93‑1 (MQ=255)
                                                             aTTTTGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGAc                           <  2:3566508/93‑1 (MQ=255)
                                                                ttGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGaa                        <  1:1531849/93‑1 (MQ=255)
                                                                ttGCGCTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGaa                        <  2:5980124/93‑1 (MQ=255)
                                                                    gcTTGCTCAATGCCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCGCCATGACGAATTaa                    <  1:2974621/93‑1 (MQ=255)
                                                                    gcTTGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTaa                    >  1:2157716/1‑93 (MQ=255)
                                                                      ttGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAAcc                  <  1:1147161/93‑1 (MQ=255)
                                                                       tGCTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACca                 >  1:3079547/1‑93 (MQ=255)
                                                                         cTCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACcaca               <  1:5823695/93‑1 (MQ=255)
                                                                          tCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAATCACAg              >  1:1486069/1‑93 (MQ=255)
                                                                          tCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAg              >  2:4418172/1‑93 (MQ=255)
                                                                          tCAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAg              <  1:1678854/93‑1 (MQ=255)
                                                                           cAATACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGt             >  2:3437520/1‑93 (MQ=255)
                                                                             aTACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCg           >  2:1919715/1‑93 (MQ=255)
                                                                             aTACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAAACACAGTCg           >  2:5501366/1‑93 (MQ=255)
                                                                              tACCGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGc          >  2:1100389/1‑93 (MQ=255)
                                                                                ccGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGcat        <  2:2234361/93‑1 (MQ=255)
                                                                                ccGCGCGCGGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGcat        <  1:1995686/93‑1 (MQ=255)
                                                                                  gcgcgcgcT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGcatca      <  2:5674898/93‑1 (MQ=255)
                                                                                  gcgcgcgGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCGCCATGACGAATTAACCACAGTCGcatca      <  2:731068/93‑1 (MQ=255)
                                                                                  gcgcgcgGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGcatca      <  1:445290/93‑1 (MQ=255)
                                                                                  gcgcgcgGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGcatca      <  2:3952745/93‑1 (MQ=255)
                                                                                   cgcgcgGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGcatcat     >  1:3126610/1‑93 (MQ=255)
                                                                                     cgccGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCCTCATGc   <  2:2644631/93‑1 (MQ=255)
                                                                                      gcgGT‑CAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGcc  >  2:4782542/1‑93 (MQ=255)
                                                                                              |                                                                                     
CCCGTTCCAGTTCACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT‑CA‑GGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCC  >  NC_000913/668959‑669136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCCAGTTCACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCA‑GGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAG  >  NC_000913/668964‑669150
                                                                                              |                                                                                             
TCCAGTTCACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCA‑GGGGGG                                                                                               >  SRR4302533.2537097/1‑94 (MQ=60)
 CCAGTTCACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCA‑GGGGGGG                                                                                              >  SRR4302533.3976847/1‑94 (MQ=60)
 CCAGTTCACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCA‑GGGGGGG                                                                                              >  SRR4302533.2131746/1‑94 (MQ=60)
  CAGTTCACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGAGCTTGCTCAATACCGCGCGCGGTCA‑GGGGGGGG                                                                                             >  SRR4302533.688569/1‑94 (MQ=60)
   AGTTCACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCA‑GGGGGGgtg                                                                                            <  SRR4302533.5898446/94‑4 (MQ=60)
   AGTTCACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCA‑GGGGGGgtg                                                                                            >  SRR4302533.5651039/1‑91 (MQ=60)
    GTTCACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGG                                                                                            <  SRR4302533.508548/94‑1 (MQ=60)
      TCACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGC                                                                                          >  SRR4302533.4770520/1‑94 (MQ=60)
      TCACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGC                                                                                          >  SRR4302533.4373608/1‑94 (MQ=60)
      TCACTGCATAAAACCAGAGCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGC                                                                                          <  SRR4302533.6041842/94‑1 (MQ=60)
        ACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGC                                                                                        >  SRR4302533.3804037/1‑94 (MQ=60)
        ACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGC                                                                                        >  SRR4302533.6324631/1‑94 (MQ=60)
        ACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGC                                                                                        >  SRR4302533.4687711/1‑94 (MQ=60)
         CTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCA                                                                                       >  SRR4302533.4038785/1‑94 (MQ=60)
         CTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCA                                                                                       >  SRR4302533.4531677/1‑94 (MQ=60)
          TGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCAT                                                                                      >  SRR4302533.2491639/1‑94 (MQ=60)
           GCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATG                                                                                     >  SRR4302533.2992018/1‑94 (MQ=60)
            CATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGA                                                                                    <  SRR4302533.6134812/94‑1 (MQ=60)
             ATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGAC                                                                                   <  SRR4302533.1078630/94‑1 (MQ=60)
             ATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGAGCATGAC                                                                                   >  SRR4302533.5299922/1‑94 (MQ=60)
               AAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCG                                                                                 <  SRR4302533.3099895/94‑1 (MQ=60)
               AAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCG                                                                                 >  SRR4302533.5522755/1‑94 (MQ=60)
                 AACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCT                                                                               <  SRR4302533.5699792/94‑1 (MQ=60)
                 AACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCT                                                                               <  SRR4302533.764858/94‑1 (MQ=60)
                 AACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCT                                                                               <  SRR4302533.3999133/94‑1 (MQ=60)
                 AACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGCGGGCGCATGACCGCT                                                                               >  SRR4302533.4878578/1‑94 (MQ=60)
                  ACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTG                                                                              >  SRR4302533.1464513/1‑94 (MQ=60)
                  ACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTG                                                                              >  SRR4302533.240520/1‑94 (MQ=60)
                   CCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGT                                                                             <  SRR4302533.3029089/94‑1 (MQ=60)
                   CCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGT                                                                             <  SRR4302533.939738/94‑1 (MQ=60)
                      GATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAA                                                                          >  SRR4302533.2131084/1‑94 (MQ=60)
                       ATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAG                                                                         >  SRR4302533.661263/1‑94 (MQ=60)
                        TCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGA                                                                        >  SRR4302533.901069/1‑94 (MQ=60)
                         CAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGAC                                                                       >  SRR4302533.3470350/1‑94 (MQ=60)
                         CAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGAC                                                                       >  SRR4302533.3436358/1‑94 (MQ=60)
                          AAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACC                                                                      >  SRR4302533.3833321/1‑94 (MQ=60)
                           AAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCA                                                                     <  SRR4302533.2965112/94‑1 (MQ=60)
                            AGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCAT                                                                    <  SRR4302533.2463040/94‑1 (MQ=60)
                              GAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCG                                                                  >  SRR4302533.3954744/1‑94 (MQ=60)
                                  CACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATAT                                                              >  SRR4302533.5609961/1‑94 (MQ=60)
                                  CACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATAT                                                              >  SRR4302533.545840/1‑94 (MQ=60)
                                    CCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACAGCTGTAAAGACCATCGATATTC                                                            >  SRR4302533.4330111/1‑94 (MQ=60)
                                     CATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCG                                                           <  SRR4302533.3944200/94‑1 (MQ=60)
                                     CATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCG                                                           <  SRR4302533.1401556/94‑1 (MQ=60)
                                      ATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGC                                                          >  SRR4302533.2156994/1‑94 (MQ=60)
                                      ATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGC                                                          >  SRR4302533.4346768/1‑94 (MQ=60)
                                      ATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGC                                                          >  SRR4302533.5143107/1‑94 (MQ=60)
                                       TGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCT                                                         <  SRR4302533.2266543/94‑1 (MQ=60)
                                       TGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCT                                                         >  SRR4302533.5676816/1‑94 (MQ=60)
                                        GTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTT                                                        >  SRR4302533.5895290/1‑94 (MQ=60)
                                         TAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTG                                                       <  SRR4302533.3989639/94‑1 (MQ=60)
                                          AGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGC                                                      <  SRR4302533.4662220/94‑1 (MQ=60)
                                           GCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCTCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCG                                                     <  SRR4302533.5606677/94‑1 (MQ=60)
                                            CAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGTGT                                                    >  SRR4302533.326437/1‑94 (MQ=60)
                                             AGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTT                                                   >  SRR4302533.5102636/1‑94 (MQ=60)
                                             AGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTT                                                   >  SRR4302533.3219355/1‑94 (MQ=60)
                                              GCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTT                                                  <  SRR4302533.3828248/94‑1 (MQ=60)
                                              GCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTT                                                  >  SRR4302533.5739543/1‑94 (MQ=60)
                                               CGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTC                                                 <  SRR4302533.361130/94‑1 (MQ=60)
                                                GTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCA                                                >  SRR4302533.998819/1‑94 (MQ=60)
                                                GTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCA                                                <  SRR4302533.2743927/94‑1 (MQ=60)
                                                GTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCA                                                >  SRR4302533.1749672/1‑94 (MQ=60)
                                                 TATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCAC                                               <  SRR4302533.489225/94‑1 (MQ=60)
                                                  ATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACC                                              >  SRR4302533.105379/1‑94 (MQ=60)
                                                   TGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCA                                             >  SRR4302533.2343832/1‑94 (MQ=60)
                                                   TGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCA                                             <  SRR4302533.2136566/94‑1 (MQ=60)
                                                    GCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCAT                                            <  SRR4302533.3653767/94‑1 (MQ=60)
                                                    GCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCAT                                            <  SRR4302533.1518199/94‑1 (MQ=60)
                                                     CAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATG                                           <  SRR4302533.5111196/94‑1 (MQ=60)
                                                     CAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATG                                           >  SRR4302533.637499/1‑94 (MQ=60)
                                                      AGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGA                                          >  SRR4302533.1354000/1‑94 (MQ=60)
                                                      AGATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGA                                          >  SRR4302533.1096449/1‑94 (MQ=60)
                                                       GATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGAC                                         <  SRR4302533.4397103/94‑1 (MQ=60)
                                                       GATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGAC                                         <  SRR4302533.3626900/94‑1 (MQ=60)
                                                        ATTTTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACG                                        >  SRR4302533.1055311/1‑94 (MQ=60)
                                                           TTGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAAT                                     <  SRR4302533.1541054/94‑1 (MQ=60)
                                                            TGCGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATT                                    <  SRR4302533.4481898/94‑1 (MQ=60)
                                                              CGCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAA                                  >  SRR4302533.2170999/1‑94 (MQ=60)
                                                               GCTTGCTCAATGCCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCGCCATGACGAATTAAC                                 <  SRR4302533.2992821/94‑1 (MQ=60)
                                                               GCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAAC                                 <  SRR4302533.319345/94‑1 (MQ=60)
                                                               GCTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAAC                                 >  SRR4302533.1589664/1‑94 (MQ=60)
                                                                CTTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACC                                >  SRR4302533.5647139/1‑94 (MQ=60)
                                                                 TTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCA                               >  SRR4302533.3098444/1‑94 (MQ=60)
                                                                 TTGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCA                               <  SRR4302533.1154007/94‑1 (MQ=60)
                                                                  TGCTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCAC                              <  SRR4302533.5285096/94‑1 (MQ=60)
                                                                    CTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAATCACAG                            >  SRR4302533.1495036/1‑94 (MQ=60)
                                                                    CTCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAG                            <  SRR4302533.5858872/94‑1 (MQ=60)
                                                                     TCAATACCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGT                           <  SRR4302533.1689023/94‑1 (MQ=60)
                                                                           CCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATC                     <  SRR4302533.2007942/94‑1 (MQ=60)
                                                                           CCGCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATC                     <  SRR4302533.96260/94‑1 (MQ=60)
                                                                             GCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCAT                   <  SRR4302533.5543032/94‑1 (MQ=60)
                                                                             GCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCAT                   <  SRR4302533.447956/94‑1 (MQ=60)
                                                                             GCGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCAT                   >  SRR4302533.3145830/1‑94 (MQ=60)
                                                                              CGCGCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATG                  >  SRR4302533.2198501/1‑94 (MQ=60)
                                                                                 GCGGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCC               <  SRR4302533.2065304/94‑1 (MQ=60)
                                                                                   GGTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTC             >  SRR4302533.5767993/1‑94 (MQ=60)
                                                                                    GTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCC            <  SRR4302533.2182192/94‑1 (MQ=60)
                                                                                    GTCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCC            <  SRR4302533.2310700/94‑1 (MQ=60)
                                                                                     TCAGGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCG           >  SRR4302533.757549/1‑94 (MQ=60)
                                                                                      cagGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGT          <  SRR4302533.5962151/91‑1 (MQ=60)
                                                                                      cagGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGT          >  SRR4302533.2477377/4‑94 (MQ=60)
                                                                                      cagGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTCTCACCGTGACGAATTAACAACAGTCGCATCATGCCCTCCGT          >  SRR4302533.2551722/4‑94 (MQ=60)
                                                                                       agGGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTA         <  SRR4302533.822621/92‑1 (MQ=60)
                                                                                       G‑GGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAA        >  SRR4302533.5960349/1‑94 (MQ=60)
                                                                                       G‑GGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAA        >  SRR4302533.3234524/1‑94 (MQ=60)
                                                                                         GGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAAC       <  SRR4302533.4045389/94‑1 (MQ=60)
                                                                                          GGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACG      <  SRR4302533.4984666/94‑1 (MQ=60)
                                                                                           GGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGA     <  SRR4302533.1476960/94‑1 (MQ=60)
                                                                                            GGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGAC    <  SRR4302533.1271988/94‑1 (MQ=60)
                                                                                             GGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACG   >  SRR4302533.5064921/1‑94 (MQ=60)
                                                                                             GGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACA   <  SRR4302533.4059899/94‑1 (MQ=60)
                                                                                              GTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAG  <  SRR4302533.4196676/94‑1 (MQ=60)
                                                                                              |                                                                                             
TCCAGTTCACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGTCA‑GGGGGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAG  >  NC_000913/668964‑669150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑