Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 697,850 T→C Q184R (CAG→CGG)  asnB ← asparagine synthetase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913697,8500TC100.0% 553.9 / NA 176Q184R (CAG→CGG) asnBasparagine synthetase B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (102/74);  total (102/74)

Only 100 of 182 total aligned reads displayed.

TGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCTGA  >  NC_000913/697760‑697939
                                                                                          |                                                                                         
    gCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTcc                                                                                     >  1:4807757/1‑93 (MQ=255)
     cAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCca                                                                                    >  2:1408513/1‑93 (MQ=255)
     cAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCca                                                                                    >  2:5142967/1‑93 (MQ=255)
     cAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCca                                                                                    >  1:2191292/1‑93 (MQ=255)
         tCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACaaa                                                                                >  1:993217/1‑93 (MQ=255)
         tCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACaaa                                                                                >  2:2399617/1‑93 (MQ=255)
         tCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACaaa                                                                                <  1:249689/93‑1 (MQ=255)
           gTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACACATa                                                                              >  2:6100378/1‑93 (MQ=255)
           gTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATa                                                                              >  1:3728527/1‑93 (MQ=255)
           gTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATa                                                                              >  1:3668982/1‑93 (MQ=255)
           gTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATa                                                                              >  1:2686383/1‑93 (MQ=255)
            tttttGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAg                                                                             >  2:6267464/1‑93 (MQ=255)
            tttttGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAg                                                                             >  2:2648248/1‑93 (MQ=255)
             ttttGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGc                                                                            <  2:5463019/93‑1 (MQ=255)
               ttGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTc                                                                          <  2:3948396/93‑1 (MQ=255)
                tGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTcc                                                                         <  1:4712062/93‑1 (MQ=255)
                tGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTcc                                                                         >  1:1648609/1‑93 (MQ=255)
                 gTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTccc                                                                        <  1:969578/93‑1 (MQ=255)
                 gTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTccc                                                                        <  2:3606189/93‑1 (MQ=255)
                   cGGTCACGTTATCGTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACCAATAGCTCCccg                                                                      >  2:6051357/1‑93 (MQ=255)
                     gTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCccgcc                                                                    >  1:5475408/1‑93 (MQ=255)
                     gTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCccgcc                                                                    >  1:4048398/1‑93 (MQ=255)
                      tCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCccgccg                                                                   <  1:5057745/93‑1 (MQ=255)
                         cGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGa                                                                >  2:5843006/1‑93 (MQ=255)
                         cGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGa                                                                >  2:4459637/1‑93 (MQ=255)
                         cGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGa                                                                >  1:1602177/1‑93 (MQ=255)
                          gTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGaa                                                               <  2:2122159/93‑1 (MQ=255)
                           ttATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGCAc                                                              >  2:3984564/1‑93 (MQ=255)
                           ttATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAAc                                                              <  1:2550771/93‑1 (MQ=255)
                            tATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAACTAGCTCCCCGCCGGGAAct                                                             >  2:527160/1‑93 (MQ=255)
                            tATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAAct                                                             <  1:6028041/93‑1 (MQ=255)
                            tATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAAct                                                             >  1:1360052/1‑93 (MQ=255)
                             ttCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAActc                                                            <  1:5136789/92‑1 (MQ=255)
                             aTCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAActc                                                            >  2:3382476/1‑93 (MQ=255)
                             aTCTTTCACCGAATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAActc                                                            <  1:3211566/93‑1 (MQ=255)
                              tCTTTCACCGCATCGTAGTCGAACCAGTCGCGATTATAGTAGGAACGGATTTCGCCGTCCCGGCTCCGCCAGTAGCTCCCCCCCGGGCAgtct                                                           >  2:5669724/1‑93 (MQ=255)
                              tCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAActct                                                           <  1:1980572/93‑1 (MQ=255)
                              tCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAActct                                                           <  1:1404140/93‑1 (MQ=255)
                               cTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCtt                                                          <  1:4312299/93‑1 (MQ=255)
                               cTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCtt                                                          <  1:5973871/93‑1 (MQ=255)
                                tttCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCttt                                                         <  1:2238770/93‑1 (MQ=255)
                                tttCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCttt                                                         <  1:358734/93‑1 (MQ=255)
                                tttCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCttt                                                         >  1:3347278/1‑93 (MQ=255)
                                    aCCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATc                                                     <  2:3430117/93‑1 (MQ=255)
                                    aCCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATc                                                     >  1:5740757/1‑93 (MQ=255)
                                    aCCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATc                                                     >  2:1987511/1‑93 (MQ=255)
                                     ccGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCg                                                    >  1:2268172/1‑93 (MQ=255)
                                     ccGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCg                                                    >  1:4890436/1‑93 (MQ=255)
                                     ccGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCg                                                    >  1:3678603/1‑93 (MQ=255)
                                      cGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGt                                                   >  2:5147236/1‑93 (MQ=255)
                                       gCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTg                                                  >  2:1238000/1‑93 (MQ=255)
                                       gCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTg                                                  <  2:6097956/93‑1 (MQ=255)
                                        cATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGc                                                 <  1:2487436/93‑1 (MQ=255)
                                        cATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGc                                                 <  2:3004371/93‑1 (MQ=255)
                                         aTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCg                                                <  1:4574362/93‑1 (MQ=255)
                                         aTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCg                                                >  2:2503996/1‑93 (MQ=255)
                                         aTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCg                                                >  1:5840586/1‑93 (MQ=255)
                                          tCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCgg                                               >  2:3597801/1‑93 (MQ=255)
                                          tCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCgg                                               >  1:4125029/1‑93 (MQ=255)
                                           cGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGc                                              <  2:4438095/93‑1 (MQ=255)
                                            gTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCa                                             >  2:1609763/1‑93 (MQ=255)
                                             tAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCaa                                            >  2:1143860/1‑93 (MQ=255)
                                             tAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCaa                                            >  2:4364263/1‑93 (MQ=255)
                                                tCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACt                                         >  2:2420854/1‑93 (MQ=255)
                                                 cGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTg                                        <  2:6038728/93‑1 (MQ=255)
                                                 cGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTg                                        <  2:3177048/93‑1 (MQ=255)
                                                  gacccAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTgg                                       <  1:303613/90‑1 (MQ=255)
                                                      cAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCGcc                                   <  2:755255/93‑1 (MQ=255)
                                                       aGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCa                                  <  1:1166184/93‑1 (MQ=255)
                                                       aGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCa                                  <  1:4001175/93‑1 (MQ=255)
                                                       aGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCa                                  <  1:5688934/93‑1 (MQ=255)
                                                         tCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAgc                                <  2:5702720/93‑1 (MQ=255)
                                                          cgcgATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAgcg                               >  2:1744082/1‑93 (MQ=255)
                                                           gcgATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAgcgc                              >  2:5427527/1‑93 (MQ=255)
                                                            cgATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCt                             <  1:5054195/93‑1 (MQ=255)
                                                             gatgatAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCtt                            >  1:1072083/1‑93 (MQ=255)
                                                              atgatAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCttt                           >  1:1514214/1‑93 (MQ=255)
                                                              atgatAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCttt                           <  2:5535969/93‑1 (MQ=255)
                                                                    gTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTc                     >  2:1689064/1‑93 (MQ=255)
                                                                     tAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAAGTCTTTAATCGTGCGGCCAACTGGCACCAGCGCTTTCATTTCt                    >  2:3347891/1‑93 (MQ=255)
                                                                     tAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCACCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCt                    <  2:5913478/93‑1 (MQ=255)
                                                                       aGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGa                  <  2:3089076/93‑1 (MQ=255)
                                                                       aGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGa                  >  2:1080395/1‑93 (MQ=255)
                                                                        gAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAg                 <  1:4532027/93‑1 (MQ=255)
                                                                          aCGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGc               <  1:3316719/93‑1 (MQ=255)
                                                                           cGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGcc              >  1:4460401/1‑93 (MQ=255)
                                                                            ggATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCca             >  1:887382/1‑93 (MQ=255)
                                                                             gATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCcac            <  1:827738/93‑1 (MQ=255)
                                                                             gATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCcac            <  2:5356812/93‑1 (MQ=255)
                                                                              aTTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCcaca           <  1:2663440/93‑1 (MQ=255)
                                                                               tttCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACAt          <  2:3864900/93‑1 (MQ=255)
                                                                               tttCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACAt          >  2:5047754/1‑93 (MQ=255)
                                                                               tttCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACAt          <  1:1091074/93‑1 (MQ=255)
                                                                                 tCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATAc        >  2:2945597/1‑93 (MQ=255)
                                                                                 tCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATAc        <  2:4072233/93‑1 (MQ=255)
                                                                                    ccGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGc     <  1:5654061/93‑1 (MQ=255)
                                                                                    ccGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGc     <  1:2985144/93‑1 (MQ=255)
                                                                                    ccGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGc     <  1:1793080/93‑1 (MQ=255)
                                                                                    ccGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGc     >  2:3582672/1‑93 (MQ=255)
                                                                                       tCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCTGa  >  1:3916894/1‑93 (MQ=255)
                                                                                          |                                                                                         
TGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCTGA  >  NC_000913/697760‑697939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCTGACCGT  >  NC_000913/697757‑697943
                                                                                             |                                                                                             
GCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCC                                                                                               >  SRR4302533.5926048/1‑94 (MQ=60)
 CCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCG                                                                                              >  SRR4302533.109344/1‑94 (MQ=60)
 CCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATAGTAGTCGAACCAGTCGCGATGGTAGTAAGAACGGATTTCGCCGTCCCG                                                                                              <  SRR4302533.2384662/94‑1 (MQ=60)
      CGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCC                                                                                         >  SRR4302533.5910451/1‑94 (MQ=60)
      CGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCC                                                                                         >  SRR4302533.4880311/1‑94 (MQ=60)
      CGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCC                                                                                         >  SRR4302533.4836865/1‑94 (MQ=60)
      CGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCC                                                                                         >  SRR4302533.4786768/1‑94 (MQ=60)
       GCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCA                                                                                        >  SRR4302533.2204794/1‑94 (MQ=60)
         AGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTAGCCGTCCCGGCTCCACA                                                                                      >  SRR4302533.3043860/1‑94 (MQ=60)
           CTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAA                                                                                    >  SRR4302533.999022/1‑94 (MQ=60)
           CTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAA                                                                                    >  SRR4302533.4714182/1‑94 (MQ=60)
            TCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAAT                                                                                   <  SRR4302533.251230/94‑1 (MQ=60)
             CGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATA                                                                                  >  SRR4302533.3751398/1‑94 (MQ=60)
             CGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATA                                                                                  >  SRR4302533.3691532/1‑94 (MQ=60)
             CGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATA                                                                                  >  SRR4302533.5692523/1‑94 (MQ=60)
             CGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATA                                                                                  >  SRR4302533.2702851/1‑94 (MQ=60)
                  TTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCC                                                                             >  SRR4302533.1658597/1‑94 (MQ=60)
                   TGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCC                                                                            <  SRR4302533.4740622/94‑1 (MQ=60)
                   TGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCC                                                                            <  SRR4302533.2510401/94‑1 (MQ=60)
                    GTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCC                                                                           <  SRR4302533.975267/94‑1 (MQ=60)
                    GTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCC                                                                           <  SRR4302533.5202333/94‑1 (MQ=60)
                     TCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCG                                                                          <  SRR4302533.4529958/94‑1 (MQ=60)
                       GGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCC                                                                        >  SRR4302533.5508411/1‑94 (MQ=60)
                       GGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCC                                                                        >  SRR4302533.4073052/1‑94 (MQ=60)
                         TCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCTCCGG                                                                      >  SRR4302533.37501/1‑94 (MQ=60)
                         TCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGG                                                                      <  SRR4302533.5088311/94‑1 (MQ=60)
                         TCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGG                                                                      <  SRR4302533.5088312/94‑1 (MQ=60)
                          CACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGG                                                                     >  SRR4302533.881924/1‑94 (MQ=60)
                           ACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGA                                                                    >  SRR4302533.1611880/1‑94 (MQ=60)
                             GTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAAC                                                                  <  SRR4302533.3700144/94‑1 (MQ=60)
                              TTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACT                                                                 >  SRR4302533.1368223/1‑94 (MQ=60)
                              TTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACT                                                                 <  SRR4302533.2566469/94‑1 (MQ=60)
                               TATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTC                                                                <  SRR4302533.6064573/94‑1 (MQ=60)
                                TTCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCT                                                               <  SRR4302533.5167828/94‑1 (MQ=60)
                                ATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCT                                                               >  SRR4302533.5766395/1‑94 (MQ=60)
                                ATCTTTCACCGAATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCT                                                               <  SRR4302533.3231349/94‑1 (MQ=60)
                                 TCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTT                                                              <  SRR4302533.1412638/94‑1 (MQ=60)
                                 TCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTT                                                              <  SRR4302533.1992697/94‑1 (MQ=60)
                                  CTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTT                                                             >  SRR4302533.408558/1‑94 (MQ=60)
                                  CTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTT                                                             <  SRR4302533.6010033/94‑1 (MQ=60)
                                  CTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTT                                                             <  SRR4302533.4338258/94‑1 (MQ=60)
                                  CTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTT                                                             >  SRR4302533.3367959/1‑94 (MQ=60)
                                   TTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTA                                                            <  SRR4302533.360917/94‑1 (MQ=60)
                                   TTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTA                                                            <  SRR4302533.2252604/94‑1 (MQ=60)
                                    TTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAA                                                           >  SRR4302533.2585965/1‑94 (MQ=60)
                                    TTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAA                                                           <  SRR4302533.1274250/94‑1 (MQ=60)
                                      CACCGCATCGTAGTCGACCCAGTCGCGATGATAGTAAGATCGTATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATC                                                         <  SRR4302533.5566033/94‑1 (MQ=60)
                                      CACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATC                                                         >  SRR4302533.5775399/1‑94 (MQ=60)
                                       ACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCG                                                        >  SRR4302533.4044609/1‑94 (MQ=60)
                                       ACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCG                                                        >  SRR4302533.2282205/1‑94 (MQ=60)
                                       ACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCG                                                        >  SRR4302533.3701215/1‑94 (MQ=60)
                                       ACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCG                                                        >  SRR4302533.3344788/1‑94 (MQ=60)
                                       ACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCG                                                        >  SRR4302533.4920066/1‑94 (MQ=60)
                                           CATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCG                                                    <  SRR4302533.2502757/94‑1 (MQ=60)
                                           CATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCG                                                    >  SRR4302533.381100/1‑94 (MQ=60)
                                           CATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCG                                                    >  SRR4302533.5875900/1‑94 (MQ=60)
                                            ATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGG                                                   >  SRR4302533.4150071/1‑94 (MQ=60)
                                            ATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGG                                                   <  SRR4302533.4602084/94‑1 (MQ=60)
                                               GTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAA                                                >  SRR4302533.936641/1‑94 (MQ=60)
                                                    CGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGG                                           <  SRR4302533.1479213/94‑1 (MQ=60)
                                                     GACCCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGC                                          <  SRR4302533.305468/94‑1 (MQ=60)
                                                     GAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGC                                          >  SRR4302533.2225705/1‑94 (MQ=60)
                                                      AACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCA                                         <  SRR4302533.5076041/94‑1 (MQ=60)
                                                        CCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACC                                       >  SRR4302533.4286917/1‑94 (MQ=60)
                                                        CCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACC                                       >  SRR4302533.2142309/1‑94 (MQ=60)
                                                          AGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAG                                     <  SRR4302533.5723295/94‑1 (MQ=60)
                                                          AGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAG                                     >  SRR4302533.1989901/1‑94 (MQ=60)
                                                          AGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAG                                     <  SRR4302533.4025538/94‑1 (MQ=60)
                                                          AGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAG                                     >  SRR4302533.140202/1‑94 (MQ=60)
                                                          AGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAG                                     >  SRR4302533.1709597/1‑94 (MQ=60)
                                                          AGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAG                                     <  SRR4302533.2556132/94‑1 (MQ=60)
                                                          AGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAG                                     <  SRR4302533.1173179/94‑1 (MQ=60)
                                                           GTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGC                                    <  SRR4302533.5685740/94‑1 (MQ=60)
                                                              GCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCT                                 >  SRR4302533.1136678/1‑94 (MQ=60)
                                                               CGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTT                                >  SRR4302533.1078399/1‑94 (MQ=60)
                                                               CGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTT                                <  SRR4302533.5084751/94‑1 (MQ=60)
                                                               CGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTT                                >  SRR4302533.2714354/1‑94 (MQ=60)
                                                                GATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTT                               >  SRR4302533.1523337/1‑94 (MQ=60)
                                                                GATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTT                               <  SRR4302533.3138269/94‑1 (MQ=60)
                                                                   GATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCAT                            <  SRR4302533.4420276/94‑1 (MQ=60)
                                                                     GAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTT                          <  SRR4302533.328515/94‑1 (MQ=60)
                                                                           GAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGG                    <  SRR4302533.4559468/94‑1 (MQ=60)
                                                                             ACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCC                  <  SRR4302533.3337202/94‑1 (MQ=60)
                                                                             ACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCC                  >  SRR4302533.4487382/1‑94 (MQ=60)
                                                                              CGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCA                 >  SRR4302533.892591/1‑94 (MQ=60)
                                                                                GATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACA               <  SRR4302533.832594/94‑1 (MQ=60)
                                                                                 ATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACAT              <  SRR4302533.2679791/94‑1 (MQ=60)
                                                                                  TTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTGATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATA             <  SRR4302533.3611415/94‑1 (MQ=60)
                                                                                  TTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATA             <  SRR4302533.1097519/94‑1 (MQ=60)
                                                                                   TTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATAC            <  SRR4302533.604144/94‑1 (MQ=60)
                                                                                    TCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACA           >  SRR4302533.2438649/1‑94 (MQ=60)
                                                                                       CCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCT        <  SRR4302533.5688233/94‑1 (MQ=60)
                                                                                       CCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCT        <  SRR4302533.1803989/94‑1 (MQ=60)
                                                                                       CCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCT        <  SRR4302533.3003406/94‑1 (MQ=60)
                                                                                       CCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCT        >  SRR4302533.6279849/1‑94 (MQ=60)
                                                                                        CGTCCCGCCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCTG       <  SRR4302533.1652446/94‑1 (MQ=60)
                                                                                         GTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCTGA      >  SRR4302533.3940818/1‑94 (MQ=60)
                                                                                          TCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCTGAC     >  SRR4302533.6209254/1‑94 (MQ=60)
                                                                                          TCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCTGAC     >  SRR4302533.3350646/1‑94 (MQ=60)
                                                                                            CCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCTGACCG   >  SRR4302533.208620/1‑94 (MQ=60)
                                                                                             CGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCTGACCGT  <  SRR4302533.5345737/94‑1 (MQ=60)
                                                                                             |                                                                                             
GCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCTGACCGT  >  NC_000913/697757‑697943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑