Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 697,850 T→C Q184R (CAG→CGG)  asnB ← asparagine synthetase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913697,8500TC90.7% 650.6 / 48.1 257Q184R (CAG→CGG) asnBasparagine synthetase B
Reads supporting (aligned to +/- strand):  ref base T (12/12);  new base C (107/126);  total (119/138)
Fisher's exact test for biased strand distribution p-value = 8.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.52e-01

Only 100 of 271 total aligned reads displayed.

CCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCTGACCG  >  NC_000913/697758‑697942
                                                                                            |                                                                                            
ccTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCt                                                                                              <  2:3094084/93‑1 (MQ=255)
   gACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGc                                                                                           >  2:6318067/1‑93 (MQ=255)
   gACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGc                                                                                           <  2:4406385/93‑1 (MQ=255)
      gCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTcc                                                                                        >  1:3522376/1‑93 (MQ=255)
      gCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTcc                                                                                        <  1:4339268/93‑1 (MQ=255)
       cAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCca                                                                                       >  1:8544743/1‑93 (MQ=255)
        aGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCcac                                                                                      >  2:527983/1‑93 (MQ=255)
        aGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCcac                                                                                      >  2:24790/1‑93 (MQ=255)
         gCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCcaca                                                                                     >  2:4581801/1‑93 (MQ=255)
          cTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACaa                                                                                    <  2:9526023/93‑1 (MQ=255)
          cTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACaa                                                                                    <  1:9310741/93‑1 (MQ=255)
          cTCGTTTTTGTAGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACaa                                                                                    >  1:4766070/1‑93 (MQ=255)
           tCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACaaa                                                                                   <  2:9724999/93‑1 (MQ=255)
           tCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACaaa                                                                                   >  1:5802965/1‑93 (MQ=255)
           tCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACaaa                                                                                   <  2:378689/93‑1 (MQ=255)
            cGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAAt                                                                                  <  2:2556973/93‑1 (MQ=255)
            cGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAAt                                                                                  <  2:1244475/93‑1 (MQ=255)
             gTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATa                                                                                 >  2:4114675/1‑93 (MQ=255)
              tttttGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAg                                                                                >  2:4608420/1‑93 (MQ=255)
               ttttGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGc                                                                               >  1:4238103/1‑93 (MQ=255)
               ttttGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGc                                                                               <  1:9798582/93‑1 (MQ=255)
                tttGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCt                                                                              >  2:3162349/1‑93 (MQ=255)
                 ttGTGGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTc                                                                             <  2:1104636/93‑1 (MQ=255)
                  tGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTcc                                                                            <  1:6082365/93‑1 (MQ=255)
                   gTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTccc                                                                           <  2:2684819/93‑1 (MQ=255)
                       gTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCccgcc                                                                       >  2:7934039/1‑93 (MQ=255)
                         cACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCgg                                                                     >  2:8779101/1‑93 (MQ=255)
                         cACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCgg                                                                     <  2:6185953/93‑1 (MQ=255)
                          aCGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCggg                                                                    <  1:1450031/93‑1 (MQ=255)
                             ttATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAAc                                                                 >  2:9287578/1‑93 (MQ=255)
                             ttATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAAc                                                                 >  2:531315/1‑93 (MQ=255)
                             ttATCTTTCACCGCATCGTAGGCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAAc                                                                 >  1:1845614/1‑93 (MQ=255)
                              tATCTTTCCCCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAAct                                                                <  1:5772557/93‑1 (MQ=255)
                              tATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAAct                                                                >  2:9924578/1‑93 (MQ=255)
                              tATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAAct                                                                >  2:921943/1‑93 (MQ=255)
                               agttttGACCGCACCGGATTGGAACCAGTCTCGATGATAGTAAGAACGGATTTGGCAGTCCTGGGTCCACAAATAGCTCCCCGACGGGAActc                                                               <  1:7704287/90‑1 (MQ=255)
                                tCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAActct                                                              <  2:3584131/93‑1 (MQ=255)
                                   ttCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTa                                                           >  2:8102870/1‑93 (MQ=255)
                                    tCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTaa                                                          >  2:5890602/1‑93 (MQ=255)
                                    tCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTaa                                                          >  2:6377460/1‑93 (MQ=255)
                                     ccccGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAAt                                                         <  1:2956981/91‑1 (MQ=255)
                                     cACCGCCTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAAt                                                         <  1:2956977/93‑1 (MQ=255)
                                     cACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAAt                                                         <  1:2626424/93‑1 (MQ=255)
                                      cccGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATc                                                        <  1:1673768/92‑1 (MQ=255)
                                      aCCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATc                                                        <  1:6417230/93‑1 (MQ=255)
                                      aCCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATc                                                        <  2:1956156/93‑1 (MQ=255)
                                         gCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTg                                                     >  1:1012773/1‑93 (MQ=255)
                                         gCATCGTAGTCGAACCAGTCGAGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTg                                                     >  2:9815415/1‑93 (MQ=255)
                                          cATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGc                                                    >  1:4823463/1‑93 (MQ=255)
                                          cATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGc                                                    <  2:1767659/93‑1 (MQ=255)
                                          cATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGc                                                    <  2:816941/93‑1 (MQ=255)
                                            tCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCgg                                                  >  2:1508479/1‑93 (MQ=255)
                                            tCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCgg                                                  >  2:7362976/1‑93 (MQ=255)
                                             cGTAGTCGAACCAGTCGCGATGATAGTAAGAACGTATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGc                                                 <  1:5868673/93‑1 (MQ=255)
                                             cGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGc                                                 >  1:9108022/1‑93 (MQ=255)
                                             cGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGc                                                 <  2:3339268/93‑1 (MQ=255)
                                              gTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCa                                                >  1:2206313/1‑93 (MQ=255)
                                               tAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCaa                                               >  2:9597233/1‑93 (MQ=255)
                                               tAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCaa                                               >  1:6299820/1‑93 (MQ=255)
                                                aGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCaaa                                              <  2:4706324/93‑1 (MQ=255)
                                                 gTCGAACCGGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAAc                                             >  1:1194918/1‑93 (MQ=255)
                                                  tCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAAcg                                            >  2:8731298/1‑92 (MQ=255)
                                                   cGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTg                                           <  1:3186030/93‑1 (MQ=255)
                                                    gAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTgg                                          >  1:2882265/1‑93 (MQ=255)
                                                    gAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTgc                                          >  1:3642022/1‑92 (MQ=255)
                                                     aaCCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGc                                         <  1:218695/93‑1 (MQ=255)
                                                     aaCCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGc                                         <  1:2751539/93‑1 (MQ=255)
                                                      aCCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAACCGTGGGGCAAACCGGcc                                        >  2:2111179/1‑92 (MQ=255)
                                                       ccAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCAc                                       >  2:8015250/1‑93 (MQ=255)
                                                         aGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCa                                     >  1:2465506/1‑93 (MQ=255)
                                                           tCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAgc                                   <  2:49124/93‑1 (MQ=255)
                                                                atgatAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCttt                              >  1:8262307/1‑93 (MQ=255)
                                                                 tgatAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTc                             >  2:1814834/1‑93 (MQ=255)
                                                                 tgatAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTc                             <  1:8138732/93‑1 (MQ=255)
                                                                  gatAGGAAGAACGGAGTTCGCCGTCCCGGCTCCACAAAGAGCGCCCCGCCGGGAACTCTTCAATCGTGGGGCAAACTGGCACCAGCGCTTTCa                            <  1:4027257/93‑1 (MQ=255)
                                                                    tAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCAtt                          <  2:9176488/93‑1 (MQ=255)
                                                                    tAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCAtt                          >  1:5457821/1‑93 (MQ=255)
                                                                     aGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCAttt                         <  1:4213066/93‑1 (MQ=255)
                                                                      gTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTc                        >  2:7601454/1‑93 (MQ=255)
                                                                      gTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCAtttt                        >  1:4512924/1‑92 (MQ=255)
                                                                        aaGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTg                      <  2:8658497/93‑1 (MQ=255)
                                                                         ataaCGAATGTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGa                     <  2:7075093/91‑1 (MQ=255)
                                                                         aGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGa                     >  1:2740092/1‑93 (MQ=255)
                                                                          gAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAg                    >  2:6973875/1‑93 (MQ=255)
                                                                          gAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAg                    >  1:7441461/1‑93 (MQ=255)
                                                                          gAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAg                    >  1:7206419/1‑93 (MQ=255)
                                                                            aCGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGc                  <  2:337256/93‑1 (MQ=255)
                                                                             cGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGcc                 >  1:9011762/1‑93 (MQ=255)
                                                                              ggATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCca                <  1:7094016/93‑1 (MQ=255)
                                                                                   tCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATAc           >  2:7171219/1‑93 (MQ=255)
                                                                                   tCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATAc           <  1:7669455/93‑1 (MQ=255)
                                                                                    cGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACa          <  1:6647010/93‑1 (MQ=255)
                                                                                    cGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACa          <  1:1651956/93‑1 (MQ=255)
                                                                                     gCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAg         <  2:8914915/93‑1 (MQ=255)
                                                                                     gCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAg         <  2:2427517/93‑1 (MQ=255)
                                                                                     gCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAg         >  1:3735458/1‑93 (MQ=255)
                                                                                       cGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCt       >  1:7956824/1‑93 (MQ=255)
                                                                                        gTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCTg      >  1:8291986/1‑93 (MQ=255)
                                                                                        gTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCTg      >  2:7518259/1‑93 (MQ=255)
                                                                                         tCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCTGa     >  2:2553759/1‑93 (MQ=255)
                                                                                            |                                                                                            
CCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCTGACCG  >  NC_000913/697758‑697942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCTGACCG  >  NC_000913/697757‑697942
                                                                                             |                                                                                            
GCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCC                                                                                              <  SRR4302540.995773/94‑1 (MQ=60)
GCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCC                                                                                              >  SRR4302540.2489282/1‑94 (MQ=60)
GCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGCCGCGATGATAGTAAGAACGGATTTCGCCGTCCC                                                                                              >  SRR4302540.5454517/1‑94 (MQ=60)
  CTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGG                                                                                            <  SRR4302540.2654177/94‑1 (MQ=60)
      CGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCC                                                                                        >  SRR4302540.3560768/1‑94 (MQ=60)
      CGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCC                                                                                        >  SRR4302540.6640948/1‑94 (MQ=60)
      CGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCC                                                                                        <  SRR4302540.7911335/94‑1 (MQ=60)
      CGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCC                                                                                        >  SRR4302540.8531047/1‑94 (MQ=60)
       GCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCA                                                                                       >  SRR4302540.8644022/1‑94 (MQ=60)
       GCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCA                                                                                       <  SRR4302540.4386507/94‑1 (MQ=60)
        GAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCAC                                                                                      <  SRR4302540.8388610/94‑1 (MQ=60)
        CAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCAC                                                                                      <  SRR4302540.574999/94‑1 (MQ=60)
         AGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACA                                                                                     >  SRR4302540.3798401/1‑94 (MQ=60)
          GCTCGTTTTTGTAGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAA                                                                                    >  SRR4302540.4816964/1‑94 (MQ=60)
           CTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAA                                                                                   <  SRR4302540.9421892/94‑1 (MQ=60)
           CTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAA                                                                                   >  SRR4302540.5867081/1‑94 (MQ=60)
           CTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAA                                                                                   >  SRR4302540.437510/1‑94 (MQ=60)
            TCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAAT                                                                                  <  SRR4302540.2214658/94‑1 (MQ=60)
             CGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATA                                                                                 <  SRR4302540.4647358/94‑1 (MQ=60)
              GTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAG                                                                                >  SRR4302540.2602397/1‑94 (MQ=60)
              GTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAG                                                                                >  SRR4302540.9508996/1‑94 (MQ=60)
               TTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGC                                                                               <  SRR4302540.2791306/94‑1 (MQ=60)
               TTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGC                                                                               >  SRR4302540.4284216/1‑94 (MQ=60)
                TTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCT                                                                              <  SRR4302540.9913326/94‑1 (MQ=60)
                 TTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTC                                                                             <  SRR4302540.575571/94‑1 (MQ=60)
                  TTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCC                                                                            >  SRR4302540.6738101/1‑94 (MQ=60)
                  TTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCC                                                                            <  SRR4302540.4327465/94‑1 (MQ=60)
                   TGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCC                                                                           <  SRR4302540.6150044/94‑1 (MQ=60)
                    GTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCC                                                                          <  SRR4302540.9326320/94‑1 (MQ=60)
                        GTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCG                                                                      <  SRR4302540.7386006/94‑1 (MQ=60)
                         TCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGG                                                                     >  SRR4302540.1753641/1‑94 (MQ=60)
                          CACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGG                                                                    >  SRR4302540.2675079/1‑94 (MQ=60)
                           ACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGA                                                                   <  SRR4302540.1465798/94‑1 (MQ=60)
                             GTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAAC                                                                 <  SRR4302540.1401089/94‑1 (MQ=60)
                             GTTATCTTTCACCGCATCGTAGGCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAAC                                                                 >  SRR4302540.1865838/1‑94 (MQ=60)
                               TATCTTTCCCCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTC                                                               <  SRR4302540.5836323/94‑1 (MQ=60)
                                agttttgaccgcaccggattgGAACCAGTCTCGATGATAGTAAGAACGGATTTGGCAGTCCTGGGTCCACAAATAGCTCCCCGACGGGAACTCT                                                              <  SRR4302540.7792483/73‑1 (MQ=60)
                                ATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCT                                                              <  SRR4302540.8381854/94‑1 (MQ=60)
                                ATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCT                                                              >  SRR4302540.3766423/1‑94 (MQ=60)
                                ATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCT                                                              <  SRR4302540.4639852/94‑1 (MQ=60)
                                 TCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTT                                                             >  SRR4302540.7750698/1‑94 (MQ=60)
                                 TCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTT                                                             <  SRR4302540.4505268/94‑1 (MQ=60)
                                  CTTTCACCGCATCGTAGTCGAGCCAGGCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTT                                                            <  SRR4302540.7703294/94‑1 (MQ=60)
                                  CTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTT                                                            >  SRR4302540.2937271/1‑94 (MQ=60)
                                      CCCCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATC                                                        <  SRR4302540.2989553/94‑1 (MQ=60)
                                      CACCGCCTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATC                                                        <  SRR4302540.2989549/94‑1 (MQ=60)
                                      CACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATC                                                        <  SRR4302540.2654832/94‑1 (MQ=60)
                                      CACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATC                                                        <  SRR4302540.2989551/94‑1 (MQ=60)
                                       CCCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCG                                                       <  SRR4302540.1691953/94‑1 (MQ=60)
                                       ACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCG                                                       <  SRR4302540.6488657/94‑1 (MQ=60)
                                       ACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCG                                                       <  SRR4302540.6488656/94‑1 (MQ=60)
                                       ACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCG                                                       <  SRR4302540.4484620/94‑1 (MQ=60)
                                        CCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGG                                                      >  SRR4302540.4211608/1‑94 (MQ=60)
                                         CGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTG                                                     >  SRR4302540.1023037/1‑94 (MQ=60)
                                          GCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGC                                                    >  SRR4302540.4875103/1‑94 (MQ=60)
                                          CCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGC                                                    <  SRR4302540.2956900/94‑1 (MQ=60)
                                           CATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCG                                                   <  SRR4302540.3500771/94‑1 (MQ=60)
                                           CATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCG                                                   >  SRR4302540.4279384/1‑94 (MQ=60)
                                            ATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGG                                                  >  SRR4302540.2496372/1‑94 (MQ=60)
                                             TCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGC                                                 >  SRR4302540.9217482/1‑94 (MQ=60)
                                              CGTAGTCGAACCAGTCGCGATGATAGTAAGAACGTATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCA                                                <  SRR4302540.5933550/94‑1 (MQ=60)
                                              CGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCA                                                >  SRR4302540.2230149/1‑94 (MQ=60)
                                              CGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCA                                                <  SRR4302540.5933549/94‑1 (MQ=60)
                                               GTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAA                                               >  SRR4302540.1791527/1‑94 (MQ=60)
                                               GTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAA                                               >  SRR4302540.6369977/1‑94 (MQ=60)
                                                TAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAA                                              <  SRR4302540.5243656/94‑1 (MQ=60)
                                                 AGTCGAACCGGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAAC                                             >  SRR4302540.1207224/1‑94 (MQ=60)
                                                 AGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAAC                                             >  SRR4302540.8279035/1‑94 (MQ=60)
                                                 AGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAAC                                             <  SRR4302540.2260528/94‑1 (MQ=60)
                                                  GTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACT                                            >  SRR4302540.3847114/1‑94 (MQ=60)
                                                  GTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACT                                            <  SRR4302540.2304456/94‑1 (MQ=60)
                                                  GTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACT                                            <  SRR4302540.1688595/94‑1 (MQ=60)
                                                   TCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAATTG                                           >  SRR4302540.3249813/1‑94 (MQ=60)
                                                   TCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTG                                           <  SRR4302540.3118562/94‑1 (MQ=60)
                                                   TCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTG                                           <  SRR4302540.4242702/94‑1 (MQ=60)
                                                   GCGAACCAGTCGCGATGATAGTAAGAAAGCATTTCTCCGGCCCGGCTCCACAAGTAGCTTCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTG                                           <  SRR4302540.3668250/94‑1 (MQ=60)
                                                    CGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGG                                          <  SRR4302540.3220647/94‑1 (MQ=60)
                                                    CGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGG                                          >  SRR4302540.2913626/1‑94 (MQ=60)
                                                    CGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGC                                          >  SRR4302540.3681910/1‑94 (MQ=60)
                                                      AACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCA                                        <  SRR4302540.2781271/94‑1 (MQ=60)
                                                      AACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCA                                        <  SRR4302540.2781272/94‑1 (MQ=60)
                                                      AACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCA                                        <  SRR4302540.220822/94‑1 (MQ=60)
                                                       ACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCAC                                       <  SRR4302540.6647471/94‑1 (MQ=60)
                                                        CCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACC                                      <  SRR4302540.5861633/94‑1 (MQ=60)
                                                        CCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACC                                      >  SRR4302540.2201399/1‑94 (MQ=60)
                                                        CCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACC                                      <  SRR4302540.3586336/94‑1 (MQ=60)
                                                         CAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCA                                     >  SRR4302540.2492041/1‑94 (MQ=60)
                                                          AGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATGGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAG                                    <  SRR4302540.7265690/94‑1 (MQ=60)
                                                           GTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGC                                   <  SRR4302540.7002690/94‑1 (MQ=60)
                                                           GTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGC                                   >  SRR4302540.9866159/1‑94 (MQ=60)
                                                           GTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGC                                   <  SRR4302540.5511636/94‑1 (MQ=60)
                                                             NNNGATGNTNGTAAGAACGGNNNNCGCCGCCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGC                                 <  SRR4302540.2019402/94‑1 (MQ=60)
                                                             CGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGC                                 >  SRR4302540.9185649/1‑94 (MQ=60)
                                                               CGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTT                               >  SRR4302540.3211566/1‑94 (MQ=60)
                                                                GATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTT                              >  SRR4302540.8358319/1‑94 (MQ=60)
                                                                GATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTT                              <  SRR4302540.8115290/94‑1 (MQ=60)
                                                                  TGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCA                            <  SRR4302540.8233197/94‑1 (MQ=60)
                                                                  TGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCA                            <  SRR4302540.8233198/94‑1 (MQ=60)
                                                                   GATAGGAAGAACGGAGTTCGCCGTCCCGGCTCCACAAAGAGCGCCCCGCCGGGAACTCTTCAATCGTGGGGCAAACTGGCACCAGCGCTTTCAT                           <  SRR4302540.4070686/94‑1 (MQ=60)
                                                                    ATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATT                          >  SRR4302540.602084/1‑94 (MQ=60)
                                                                    ATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATT                          >  SRR4302540.156285/1‑94 (MQ=60)
                                                                    ATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATT                          >  SRR4302540.5517890/1‑94 (MQ=60)
                                                                    ATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATT                          >  SRR4302540.156286/1‑94 (MQ=60)
                                                                      AGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTT                        >  SRR4302540.4561820/1‑94 (MQ=60)
                                                                      AGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTC                        <  SRR4302540.4258937/94‑1 (MQ=60)
                                                                      AGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTC                        <  SRR4302540.6229368/94‑1 (MQ=60)
                                                                       GTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCT                       <  SRR4302540.8308087/94‑1 (MQ=60)
                                                                         AAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGA                     >  SRR4302540.2769677/1‑94 (MQ=60)
                                                                          AGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAG                    >  SRR4302540.7524372/1‑94 (MQ=60)
                                                                          AGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAG                    >  SRR4302540.7286758/1‑94 (MQ=60)
                                                                           GAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGG                   <  SRR4302540.4843046/94‑1 (MQ=60)
                                                                           GAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGG                   >  SRR4302540.9253152/1‑94 (MQ=60)
                                                                            AACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGC                  >  SRR4302540.1194932/1‑94 (MQ=60)
                                                                             ACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCC                 >  SRR4302540.9119869/1‑94 (MQ=60)
                                                                               GGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCAC               <  SRR4302540.7172960/94‑1 (MQ=60)
                                                                                GATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACA              <  SRR4302540.8970439/94‑1 (MQ=60)
                                                                                 ATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACAT             >  SRR4302540.2744214/1‑94 (MQ=60)
                                                                                   TTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATAC           <  SRR4302540.3593332/94‑1 (MQ=60)
                                                                                   TTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATAC           >  SRR4302540.7475566/1‑94 (MQ=60)
                                                                                    TCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACA          <  SRR4302540.7755910/94‑1 (MQ=60)
                                                                                    TCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACA          <  SRR4302540.7755909/94‑1 (MQ=60)
                                                                                     CGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAG         <  SRR4302540.6720548/94‑1 (MQ=60)
                                                                                     CGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAG         <  SRR4302540.1669965/94‑1 (MQ=60)
                                                                                     CGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAG         >  SRR4302540.3776216/1‑94 (MQ=60)
                                                                                       CCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCT       >  SRR4302540.8049570/1‑94 (MQ=60)
                                                                                       CCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCT       >  SRR4302540.8049571/1‑94 (MQ=60)
                                                                                        CGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCTG      >  SRR4302540.8388280/1‑94 (MQ=60)
                                                                                        CGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCTG      >  SRR4302540.2019870/1‑94 (MQ=60)
                                                                                        CGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCTG      <  SRR4302540.9040085/94‑1 (MQ=60)
                                                                                        CGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCTG      <  SRR4302540.5978209/94‑1 (MQ=60)
                                                                                        CGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCTG      <  SRR4302540.1387060/94‑1 (MQ=60)
                                                                                         GTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATCCAGCTac     >  SRR4302540.2862087/1‑92 (MQ=60)
                                                                                         GTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCTGA     <  SRR4302540.5875822/94‑1 (MQ=60)
                                                                                           CCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGGGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCTCACC   >  SRR4302540.2281143/1‑94 (MQ=60)
                                                                                            CTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCTGACCG  >  SRR4302540.6175245/1‑94 (MQ=60)
                                                                                            CCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCTGACCG  >  SRR4302540.8054171/1‑94 (MQ=60)
                                                                                             |                                                                                            
GCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCTGACCG  >  NC_000913/697757‑697942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑