Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 709,880 T→C intergenic (‑11/+278) fur ← / ← fldA Fur/flavodoxin 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913709,8800TC99.5% 690.4 / NA 212intergenic (‑11/+278)fur/fldAFur/flavodoxin 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (121/90);  minor base A (1/0);  total (122/90)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 217 total aligned reads displayed.

TGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAA  >  NC_000913/709790‑709969
                                                                                          |                                                                                         
 gaagaaCTTCCAGGATTTTTAAACGAGGAAGCGTTCCTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCt                                                                                        <  1:2522321/93‑1 (MQ=255)
 gaagaaCTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCt                                                                                        >  1:2311767/1‑93 (MQ=255)
 gaagaaCTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCt                                                                                        >  2:5681465/1‑93 (MQ=255)
    gaaCTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGtt                                                                                     >  2:5006608/1‑93 (MQ=255)
      aCTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTAc                                                                                   <  2:3697897/93‑1 (MQ=255)
       cTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACt                                                                                  >  1:5303692/1‑93 (MQ=255)
         tCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTaa                                                                                <  1:3366194/93‑1 (MQ=255)
          ccAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAg                                                                               >  1:1197292/1‑93 (MQ=255)
          ccAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAg                                                                               <  2:5113829/93‑1 (MQ=255)
           cAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGc                                                                              >  2:4918911/1‑93 (MQ=255)
           cAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGAATTGTTATCAGCCATGCGGAATCTGCCCTGTTACTAAgg                                                                              >  1:6194442/1‑92 (MQ=255)
             ggATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCgg                                                                            >  1:1388143/1‑93 (MQ=255)
              gATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGt                                                                           >  1:1491857/1‑93 (MQ=255)
              gATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGt                                                                           <  1:4322295/93‑1 (MQ=255)
               aTTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGtt                                                                          >  1:4439538/1‑93 (MQ=255)
                tttttAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTc                                                                         <  1:6258782/93‑1 (MQ=255)
                  tttAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCAc                                                                       <  2:6177752/93‑1 (MQ=255)
                  tttAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCAc                                                                       <  2:461765/93‑1 (MQ=255)
                    tAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACtt                                                                     <  2:855559/93‑1 (MQ=255)
                     aaaCGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTc                                                                    >  1:5203009/1‑93 (MQ=255)
                     aaaCGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTc                                                                    >  2:1501333/1‑93 (MQ=255)
                     aaaCGAGGAAGCGTTACTTTCACGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTc                                                                    >  1:5099356/1‑93 (MQ=255)
                     aaaCAAGGAAGCGTTTCTTGCACGCCAGCTGTCGTGAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTc                                                                    <  1:3557517/93‑1 (MQ=255)
                      aaCGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCa                                                                   >  2:1882103/1‑93 (MQ=255)
                       aCGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCAt                                                                  <  2:162165/93‑1 (MQ=255)
                       aCGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCAt                                                                  >  2:2193828/1‑93 (MQ=255)
                       aCGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTACACTTCAt                                                                  >  1:4652085/1‑93 (MQ=255)
                          aGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTag                                                               >  2:4150978/1‑93 (MQ=255)
                           ggAAGCGTTTCTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTaga                                                              <  2:126481/93‑1 (MQ=255)
                           ggAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTaga                                                              >  2:2234241/1‑93 (MQ=255)
                            gAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTagaa                                                             >  1:3085313/1‑93 (MQ=255)
                            gAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTagaa                                                             <  1:5005152/93‑1 (MQ=255)
                            gAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTagaa                                                             >  1:3591546/1‑93 (MQ=255)
                             aaGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTagaag                                                            <  2:2426080/93‑1 (MQ=255)
                             aaGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTagaag                                                            <  2:760060/93‑1 (MQ=255)
                               gCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAgaagaa                                                          >  2:2147935/1‑93 (MQ=255)
                               gCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAgaagaa                                                          >  2:2953524/1‑93 (MQ=255)
                                cGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGaagaag                                                         >  1:4270924/1‑93 (MQ=255)
                                cGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGaagaag                                                         <  2:6078281/93‑1 (MQ=255)
                                 gTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGt                                                        >  1:800443/1‑93 (MQ=255)
                                  ttACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTg                                                       >  1:3002019/1‑93 (MQ=255)
                                  ttACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTg                                                       >  2:5931758/1‑93 (MQ=255)
                                  ttACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTg                                                       >  1:2631801/1‑93 (MQ=255)
                                   tACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGTAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGa                                                      <  1:5739040/93‑1 (MQ=255)
                                   tACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGa                                                      <  1:4201006/93‑1 (MQ=255)
                                     cTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACa                                                    >  1:3777832/1‑93 (MQ=255)
                                     cTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACa                                                    <  1:610647/93‑1 (MQ=255)
                                     cTTTCAGGCCAGCTTTCGTTAGGTCGGTATTGTTATCAGTCATGCTTAATCTGCCCTGGTGCTAAGCGGTTCACTTCATTAGAAGAAGTGACa                                                    <  1:4945653/93‑1 (MQ=255)
                                      tttCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAg                                                   >  2:4064861/1‑93 (MQ=255)
                                      tttCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAg                                                   >  2:4882135/1‑93 (MQ=255)
                                      tttCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAg                                                   >  2:563052/1‑93 (MQ=255)
                                       ttCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGa                                                  <  1:466629/93‑1 (MQ=255)
                                        tCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGaa                                                 >  2:4657301/1‑93 (MQ=255)
                                          aGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAAtt                                               <  1:4562138/93‑1 (MQ=255)
                                          aGGCCAGCTTTCTTTAGGGCGGAATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAAtt                                               >  2:2837564/1‑93 (MQ=255)
                                           ggCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAAttt                                              <  2:4685769/93‑1 (MQ=255)
                                             ccAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGc                                            <  2:1652563/93‑1 (MQ=255)
                                              cAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGct                                           >  2:2259184/1‑93 (MQ=255)
                                               aGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGgtc                                          >  1:214384/1‑93 (MQ=255)
                                                 ctttctttAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCtt                                        >  1:2190537/1‑93 (MQ=255)
                                                  tttctttAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTg                                       >  1:5590670/1‑93 (MQ=255)
                                                  tttctttAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTg                                       >  2:49744/1‑93 (MQ=255)
                                                  tttctttAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTg                                       >  1:1014689/1‑93 (MQ=255)
                                                   ttctttAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTga                                      <  2:3538853/93‑1 (MQ=255)
                                                   ttctttAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTga                                      <  1:1987409/93‑1 (MQ=255)
                                                     ctttAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTgaga                                    <  2:4479888/93‑1 (MQ=255)
                                                      tttAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGAt                                   >  2:1409100/1‑93 (MQ=255)
                                                       ttAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATa                                  >  2:1178199/1‑93 (MQ=255)
                                                        tAGGGCGGTATTGTTATCAGTCATGCGGAATCTCCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATaa                                 >  2:3486960/1‑93 (MQ=255)
                                                         aGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAAt                                <  1:4778783/93‑1 (MQ=255)
                                                         aGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAAt                                <  1:3179584/93‑1 (MQ=255)
                                                              ggTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTa                           <  1:5444930/93‑1 (MQ=255)
                                                              ggTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTa                           >  2:4331315/1‑93 (MQ=255)
                                                              ggTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTa                           >  2:5279400/1‑93 (MQ=255)
                                                               gTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTAt                          >  1:5856786/1‑93 (MQ=255)
                                                               gTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTAt                          >  2:3909891/1‑93 (MQ=255)
                                                               gTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTAt                          >  1:5541605/1‑93 (MQ=255)
                                                               gTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTAt                          >  1:1575177/1‑93 (MQ=255)
                                                               gTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTAt                          >  1:512048/1‑93 (MQ=255)
                                                                  ttGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCAt                       >  2:4757051/1‑93 (MQ=255)
                                                                   tGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCAtt                      >  1:5628167/1‑93 (MQ=255)
                                                                    gTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATta                     <  1:6004730/93‑1 (MQ=255)
                                                                    gTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATta                     >  1:4738486/1‑93 (MQ=255)
                                                                    gTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATta                     <  1:4047122/93‑1 (MQ=255)
                                                                    gTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATta                     >  2:2857354/1‑93 (MQ=255)
                                                                    gTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATta                     <  2:2587956/93‑1 (MQ=255)
                                                                        tCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGa                 >  2:4256865/1‑93 (MQ=255)
                                                                          aGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAAt               <  2:2934572/93‑1 (MQ=255)
                                                                          aGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAAt               >  2:907254/1‑93 (MQ=255)
                                                                           gTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAAtt              >  1:384722/1‑93 (MQ=255)
                                                                           gTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAAtt              >  1:1431524/1‑93 (MQ=255)
                                                                           atCATGCGAAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAAtt              <  1:986043/92‑1 (MQ=255)
                                                                               tGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCa          >  1:5913145/1‑93 (MQ=255)
                                                                                gCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCAc         <  2:4401180/93‑1 (MQ=255)
                                                                                   gAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGcc      >  2:5820998/1‑93 (MQ=255)
                                                                                    aaTCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCt     >  1:1807200/1‑93 (MQ=255)
                                                                                    aaTCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCt     >  2:815697/1‑93 (MQ=255)
                                                                                     aTCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTa    >  1:5792202/1‑93 (MQ=255)
                                                                                      tCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTaa   >  1:1887326/1‑93 (MQ=255)
                                                                                      tCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTaa   >  2:716827/1‑93 (MQ=255)
                                                                                          |                                                                                         
TGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAA  >  NC_000913/709790‑709969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATG  >  NC_000913/709788‑709971
                                                                                            |                                                                                           
CCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCC                                                                                            >  SRR4302533.1683223/1‑94 (MQ=60)
  TGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCT                                                                                          >  SRR4302533.2326088/1‑94 (MQ=60)
  TGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCT                                                                                          >  SRR4302533.4964178/1‑94 (MQ=60)
   GAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTCCTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTG                                                                                         <  SRR4302533.2537862/94‑1 (MQ=60)
   GAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGACCTG                                                                                         >  SRR4302533.1218196/1‑94 (MQ=60)
     AGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTT                                                                                       >  SRR4302533.627611/1‑94 (MQ=60)
     AGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTT                                                                                       >  SRR4302533.4768216/1‑94 (MQ=60)
        ACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACT                                                                                    <  SRR4302533.2642967/94‑1 (MQ=60)
        ACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACT                                                                                    >  SRR4302533.5335467/1‑94 (MQ=60)
         CTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTA                                                                                   <  SRR4302533.4070386/94‑1 (MQ=60)
           TCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAG                                                                                 <  SRR4302533.3386987/94‑1 (MQ=60)
           TCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAG                                                                                 >  SRR4302533.1204487/1‑94 (MQ=60)
           TCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAG                                                                                 >  SRR4302533.6337688/1‑94 (MQ=60)
            CCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGAATTGTTATCAGCCATGCGGAATCTGCCCTGTTACTAAGG                                                                                >  SRR4302533.6231610/1‑94 (MQ=60)
             CAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCG                                                                               >  SRR4302533.1433927/1‑94 (MQ=60)
              AGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGG                                                                              >  SRR4302533.886580/1‑94 (MQ=60)
              AGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGG                                                                              >  SRR4302533.1396501/1‑94 (MQ=60)
               GGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGT                                                                             >  SRR4302533.1500853/1‑94 (MQ=60)
                GATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTT                                                                            <  SRR4302533.4348325/94‑1 (MQ=60)
                GATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTT                                                                            >  SRR4302533.4466373/1‑94 (MQ=60)
                  TTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCA                                                                          <  SRR4302533.6296206/94‑1 (MQ=60)
                  TTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCA                                                                          <  SRR4302533.3261768/94‑1 (MQ=60)
                   TTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCAC                                                                         <  SRR4302533.3525232/94‑1 (MQ=60)
                     TTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTT                                                                       >  SRR4302533.3703340/1‑94 (MQ=60)
                      TAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTC                                                                      >  SRR4302533.5234365/1‑94 (MQ=60)
                      TAAACGAGGAAGCGTTACTTTCACGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTC                                                                      >  SRR4302533.5130164/1‑94 (MQ=60)
                       AAACAAGGAAGCGTTTCTTGCACGCCAGCTGTCGTGAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCA                                                                     <  SRR4302533.3579438/94‑1 (MQ=60)
                        AACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCAT                                                                    <  SRR4302533.1148209/94‑1 (MQ=60)
                        AACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCAT                                                                    <  SRR4302533.1509724/94‑1 (MQ=60)
                        AACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTACACTTCAT                                                                    >  SRR4302533.4680275/1‑94 (MQ=60)
                          CGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTA                                                                  <  SRR4302533.5041969/94‑1 (MQ=60)
                            AGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGA                                                                <  SRR4302533.5170497/94‑1 (MQ=60)
                             GGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAA                                                               >  SRR4302533.3104284/1‑94 (MQ=60)
                             GGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAA                                                               >  SRR4302533.5649406/1‑94 (MQ=60)
                             GGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAA                                                               >  SRR4302533.3613653/1‑94 (MQ=60)
                              GAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAG                                                              <  SRR4302533.5035485/94‑1 (MQ=60)
                                 GCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAG                                                           >  SRR4302533.4296631/1‑94 (MQ=60)
                                 GCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAG                                                           >  SRR4302533.5398176/1‑94 (MQ=60)
                                  CGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGT                                                          >  SRR4302533.805153/1‑94 (MQ=60)
                                   GTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTG                                                         >  SRR4302533.2647973/1‑94 (MQ=60)
                                   GTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTG                                                         >  SRR4302533.3020377/1‑94 (MQ=60)
                                     TACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGTAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGAC                                                       <  SRR4302533.5773674/94‑1 (MQ=60)
                                     TACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGAC                                                       <  SRR4302533.4226426/94‑1 (MQ=60)
                                      ACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACA                                                      >  SRR4302533.3800980/1‑94 (MQ=60)
                                       CTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAG                                                     <  SRR4302533.614236/94‑1 (MQ=60)
                                       CTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAG                                                     <  SRR4302533.5534106/94‑1 (MQ=60)
                                       CTTTCAGGCCAGCTTTCGTTAGGTCGGTATTGTTATCAGTCATGCTTAATCTGCCCTGGTGCTAAGCGGTTCACTTCATTAGAAGAAGTGACAG                                                     <  SRR4302533.4975587/94‑1 (MQ=60)
                                        TTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGA                                                    >  SRR4302533.6108982/1‑94 (MQ=60)
                                        TTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGA                                                    <  SRR4302533.5580538/94‑1 (MQ=60)
                                        TTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGA                                                    >  SRR4302533.4723159/1‑94 (MQ=60)
                                         TTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAA                                                   >  SRR4302533.358673/1‑94 (MQ=60)
                                         TTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAA                                                   <  SRR4302533.469417/94‑1 (MQ=60)
                                            AGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTT                                                <  SRR4302533.4589804/94‑1 (MQ=60)
                                            AGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTT                                                <  SRR4302533.5662915/94‑1 (MQ=60)
                                            AGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTT                                                <  SRR4302533.1464375/94‑1 (MQ=60)
                                            AGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTT                                                <  SRR4302533.5175893/94‑1 (MQ=60)
                                               CCAGCTTTCTTTAGGGCGGTATTGTTATCAGTTATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCT                                             <  SRR4302533.5821812/94‑1 (MQ=60)
                                                CAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGGTC                                            >  SRR4302533.215733/1‑94 (MQ=60)
                                                CAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTC                                            >  SRR4302533.5025104/1‑94 (MQ=60)
                                                 AGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCT                                           >  SRR4302533.2242012/1‑94 (MQ=60)
                                                 AGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCT                                           <  SRR4302533.4662217/94‑1 (MQ=60)
                                                  GCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTT                                          >  SRR4302533.1744004/1‑94 (MQ=60)
                                                  GCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTT                                          <  SRR4302533.6022015/94‑1 (MQ=60)
                                                  GCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTT                                          >  SRR4302533.2204032/1‑94 (MQ=60)
                                                  GCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTT                                          >  SRR4302533.3038114/1‑94 (MQ=60)
                                                  GCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTT                                          <  SRR4302533.5711064/94‑1 (MQ=60)
                                                   CTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTG                                         >  SRR4302533.1020649/1‑94 (MQ=60)
                                                   CTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTG                                         >  SRR4302533.5624441/1‑94 (MQ=60)
                                                     TTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAG                                       <  SRR4302533.1999565/94‑1 (MQ=60)
                                                     TTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAG                                       >  SRR4302533.5837412/1‑94 (MQ=60)
                                                      TCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGA                                      >  SRR4302533.4261960/1‑94 (MQ=60)
                                                       CTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGAT                                     <  SRR4302533.1609551/94‑1 (MQ=60)
                                                          TAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAAT                                  >  SRR4302533.6195457/1‑94 (MQ=60)
                                                          TAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAAT                                  >  SRR4302533.2031375/1‑94 (MQ=60)
                                                          TAGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAAT                                  >  SRR4302533.2368471/1‑94 (MQ=60)
                                                           AGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATG                                 <  SRR4302533.4807728/94‑1 (MQ=60)
                                                           AGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATG                                 <  SRR4302533.3199143/94‑1 (MQ=60)
                                                           AGGGCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATG                                 <  SRR4302533.1852413/94‑1 (MQ=60)
                                                              GCGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGT                              <  SRR4302533.4150600/94‑1 (MQ=60)
                                                               CGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTA                             <  SRR4302533.2191639/94‑1 (MQ=60)
                                                               CGGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTA                             >  SRR4302533.2698390/1‑94 (MQ=60)
                                                                GGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTAT                            >  SRR4302533.3806815/1‑94 (MQ=60)
                                                                GGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTAT                            >  SRR4302533.5892192/1‑94 (MQ=60)
                                                                GGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTAT                            >  SRR4302533.1584663/1‑94 (MQ=60)
                                                                GGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTAT                            <  SRR4302533.5477736/94‑1 (MQ=60)
                                                                GGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTAT                            >  SRR4302533.5575070/1‑94 (MQ=60)
                                                                GGTATTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTAT                            >  SRR4302533.515091/1‑94 (MQ=60)
                                                                    TTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATT                        >  SRR4302533.5662196/1‑94 (MQ=60)
                                                                    TTGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATT                        <  SRR4302533.6171453/94‑1 (MQ=60)
                                                                     TGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTA                       >  SRR4302533.1922130/1‑94 (MQ=60)
                                                                     TGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTA                       <  SRR4302533.2500534/94‑1 (MQ=60)
                                                                     TGTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTA                       >  SRR4302533.4767218/1‑94 (MQ=60)
                                                                      GTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTAT                      >  SRR4302533.423029/1‑94 (MQ=60)
                                                                      GTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTAT                      >  SRR4302533.5898091/1‑94 (MQ=60)
                                                                      GTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTAT                      <  SRR4302533.6041112/94‑1 (MQ=60)
                                                                      GTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTAT                      <  SRR4302533.4071766/94‑1 (MQ=60)
                                                                      GTTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTAT                      >  SRR4302533.4689666/1‑94 (MQ=60)
                                                                       TTATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATA                     <  SRR4302533.5138846/94‑1 (MQ=60)
                                                                        TATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAG                    >  SRR4302533.3522714/1‑94 (MQ=60)
                                                                        TATCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAG                    <  SRR4302533.4523190/94‑1 (MQ=60)
                                                                          TCAGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAA                  <  SRR4302533.4735771/94‑1 (MQ=60)
                                                                            AGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATT                >  SRR4302533.387044/1‑94 (MQ=60)
                                                                            AGTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATT                >  SRR4302533.1440175/1‑94 (MQ=60)
                                                                             GTCATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTG               >  SRR4302533.3124386/1‑94 (MQ=60)
                                                                             ATCATGCGAAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTG               <  SRR4302533.991813/94‑1 (MQ=60)
                                                                                ATGCGGAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCA            >  SRR4302533.5948876/1‑94 (MQ=60)
                                                                                     GAATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCT       >  SRR4302533.1818197/1‑94 (MQ=60)
                                                                                      AATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTA      >  SRR4302533.5827193/1‑94 (MQ=60)
                                                                                       ATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAA     >  SRR4302533.5251977/1‑94 (MQ=60)
                                                                                       ATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAA     <  SRR4302533.4405249/94‑1 (MQ=60)
                                                                                       ATCTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAA     >  SRR4302533.1898853/1‑94 (MQ=60)
                                                                                         CTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAAT   >  SRR4302533.5566727/1‑94 (MQ=60)
                                                                                         CTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAAT   >  SRR4302533.5549841/1‑94 (MQ=60)
                                                                                         CTGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAAT   <  SRR4302533.4310804/94‑1 (MQ=60)
                                                                                          TGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATG  >  SRR4302533.5133820/1‑94 (MQ=60)
                                                                                          TGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATG  >  SRR4302533.4206307/1‑94 (MQ=60)
                                                                                          TGCCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATG  >  SRR4302533.2554097/1‑94 (MQ=60)
                                                                                            |                                                                                           
CCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGGGCGGTATTGTTATCAGTCATGCGGAATCTGTCCTGTTACTAAGCGGTTCACTTCATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATG  >  NC_000913/709788‑709971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑