Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 772,731 A→G Y156C (TAC→TGC)  cydB → cytochrome d terminal oxidase polypeptide subunit II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913772,7310AG100.0% 759.8 / NA 231Y156C (TAC→TGC) cydBcytochrome d terminal oxidase polypeptide subunit II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (123/108);  total (123/108)

Only 100 of 238 total aligned reads displayed.

GTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTACTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTACTCAGGGCGCAA  >  NC_000913/772641‑772820
                                                                                          |                                                                                         
gTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGtgct                                                                                         >  1:2027572/1‑93 (MQ=255)
   gCTTCGTTCCGCCGCTGTTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTaca                                                                                      <  2:2413723/93‑1 (MQ=255)
    cTTCGTTCCGCCGCTGGTGATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTacac                                                                                     >  2:2047334/1‑93 (MQ=255)
     ttCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACAcc                                                                                    <  2:6275732/93‑1 (MQ=255)
     ttCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACAcc                                                                                    >  2:2582124/1‑93 (MQ=255)
      tCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCg                                                                                   >  2:3183631/1‑93 (MQ=255)
       cGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCgg                                                                                  <  1:6248696/93‑1 (MQ=255)
       cGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCgg                                                                                  >  1:198163/1‑93 (MQ=255)
        gTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGt                                                                                 <  1:2183493/93‑1 (MQ=255)
         ttCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTa                                                                                <  2:1046033/93‑1 (MQ=255)
            cgccgcTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAAct                                                                             >  1:3593483/1‑93 (MQ=255)
             gccgcTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAActt                                                                            >  2:3210657/1‑93 (MQ=255)
              ccgcTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAActtc                                                                           <  2:3442886/93‑1 (MQ=255)
              ccgcTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAActtc                                                                           <  1:5085216/93‑1 (MQ=255)
                 cTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACttcttc                                                                        >  2:2432379/1‑93 (MQ=255)
                 cTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACttcttc                                                                        <  2:5450904/93‑1 (MQ=255)
                 cTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACttcttc                                                                        >  1:1679650/1‑93 (MQ=255)
                  tGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTcc                                                                       <  1:5119466/93‑1 (MQ=255)
                  tGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTcc                                                                       <  1:70242/93‑1 (MQ=255)
                  tGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTcc                                                                       <  1:3676944/93‑1 (MQ=255)
                  tGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTcc                                                                       <  1:3740997/93‑1 (MQ=255)
                   ggTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCa                                                                      <  2:3628283/93‑1 (MQ=255)
                     tAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGt                                                                    <  1:149171/93‑1 (MQ=255)
                     tAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGt                                                                    >  1:400836/1‑93 (MQ=255)
                     tAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCCTCCAGt                                                                    >  1:6149268/1‑93 (MQ=255)
                      aaTTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGtt                                                                   >  1:371888/1‑93 (MQ=255)
                      aaTTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGtt                                                                   <  1:3188590/93‑1 (MQ=255)
                        ttGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGc                                                                 >  2:5185536/1‑93 (MQ=255)
                          ggTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCtt                                                               >  2:6218031/1‑93 (MQ=255)
                           gtgtAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTa                                                              >  2:777454/1‑93 (MQ=255)
                            tgtAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTaa                                                             >  2:651009/1‑93 (MQ=255)
                             gtAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAAc                                                            <  2:1127918/93‑1 (MQ=255)
                             gtAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAAc                                                            <  2:61257/93‑1 (MQ=255)
                               aGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAAccc                                                          <  1:4742998/93‑1 (MQ=255)
                                gCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCg                                                         >  2:4572615/1‑93 (MQ=255)
                                gCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCg                                                         >  2:2123655/1‑93 (MQ=255)
                                   ttCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACCCCGGTAACTTCTTCCAGTTGCTTAACCCGTTc                                                      <  2:2847809/93‑1 (MQ=255)
                                   ttCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTc                                                      <  1:3259361/93‑1 (MQ=255)
                                   ttCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTc                                                      >  1:3642376/1‑93 (MQ=255)
                                    tCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCg                                                     <  1:2417809/93‑1 (MQ=255)
                                    tCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCg                                                     <  2:1744071/93‑1 (MQ=255)
                                     cGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCgg                                                    >  1:2770668/1‑93 (MQ=255)
                                      ggTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGc                                                   >  1:4219375/1‑93 (MQ=255)
                                      ggTAACCTGTTGCAGCGCGTACTGTTCAACGTTGATGAATATCTGTGTCTGTGCTACTGCTGTAACTTCTTCCAGTTGCTTAACCCGTTCGGc                                                   <  2:2739249/93‑1 (MQ=255)
                                       gTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCTGcc                                                  >  2:4796842/1‑93 (MQ=255)
                                       gTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGcc                                                  <  2:3569864/93‑1 (MQ=255)
                                        tAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCct                                                 >  2:6069316/1‑93 (MQ=255)
                                         aaCCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCctg                                                >  1:4866120/1‑93 (MQ=255)
                                         aaCCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCctg                                                <  2:4847128/93‑1 (MQ=255)
                                         aaCCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCctg                                                >  1:2529995/1‑93 (MQ=255)
                                          aCCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGGctgc                                               >  2:1613557/1‑93 (MQ=255)
                                          aCCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCctgc                                               >  2:4465152/1‑93 (MQ=255)
                                          aCCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCctgc                                               <  1:1092709/93‑1 (MQ=255)
                                           ccTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCctgct                                              <  2:83363/93‑1 (MQ=255)
                                             tGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTgg                                            >  1:3948137/1‑93 (MQ=255)
                                             tGTTGCAGGGCGGACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTgg                                            >  1:3403750/1‑93 (MQ=255)
                                              gTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGc                                           <  2:3650930/93‑1 (MQ=255)
                                                  cAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGc                                       >  2:5212993/1‑93 (MQ=255)
                                                   aGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCg                                      >  2:5844213/1‑93 (MQ=255)
                                                   aGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCg                                      >  1:932405/1‑93 (MQ=255)
                                                   aGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCg                                      >  2:233439/1‑93 (MQ=255)
                                                    gggCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCgt                                     >  1:1260614/1‑93 (MQ=255)
                                                     ggCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCgtg                                    >  2:3788311/1‑93 (MQ=255)
                                                       cGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCgtggt                                  >  2:1348877/1‑93 (MQ=255)
                                                       cGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCgtggg                                  >  1:2696292/1‑92 (MQ=255)
                                                         tACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGa                                <  2:1218311/93‑1 (MQ=255)
                                                         tACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGa                                >  2:3004313/1‑93 (MQ=255)
                                                         tACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCACTTGCTTAACCCGCTCGGCCTGCTGGCCGGCGTGgaga                                >  2:4162256/1‑93 (MQ=255)
                                                          aCCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAg                               >  1:2563427/1‑93 (MQ=255)
                                                          aCCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAg                               >  1:2984208/1‑93 (MQ=255)
                                                             gTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGt                            >  1:5667515/1‑93 (MQ=255)
                                                              ttCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTa                           <  1:5264260/93‑1 (MQ=255)
                                                              ttCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTa                           >  2:1849653/1‑93 (MQ=255)
                                                              ttCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTa                           <  1:6014876/93‑1 (MQ=255)
                                                              ttCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTa                           <  1:5947751/93‑1 (MQ=255)
                                                               tCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAg                          >  1:4490734/1‑93 (MQ=255)
                                                                 aaCGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAggg                        >  1:38523/1‑93 (MQ=255)
                                                                 aaCGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAggg                        >  2:4996508/1‑93 (MQ=255)
                                                                  aCGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGga                       >  2:5639074/1‑93 (MQ=255)
                                                                  aCGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGga                       <  2:5329371/93‑1 (MQ=255)
                                                                      tgatgaATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGgatgat                   <  2:4289797/93‑1 (MQ=255)
                                                                        atgaATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCa                 <  2:5356021/93‑1 (MQ=255)
                                                                         tgaATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCAt                >  1:1323933/1‑93 (MQ=255)
                                                                         tgaATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCAt                >  1:1579191/1‑93 (MQ=255)
                                                                           aaTATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTa              >  2:5730090/1‑93 (MQ=255)
                                                                            atatCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTAc             >  1:4610274/1‑93 (MQ=255)
                                                                            atatCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTAc             <  1:5810571/93‑1 (MQ=255)
                                                                             tatCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTACt            >  1:845670/1‑93 (MQ=255)
                                                                              atCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAGCCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTACTc           <  1:3916599/93‑1 (MQ=255)
                                                                              atCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTACTc           <  2:3358644/93‑1 (MQ=255)
                                                                               tCTGCGTCTGTGCTACACCGTTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTACTCa          <  2:3393479/93‑1 (MQ=255)
                                                                               tCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTACTCa          <  2:1454285/93‑1 (MQ=255)
                                                                               tCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTACTCa          <  1:4540643/93‑1 (MQ=255)
                                                                                   cGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTACTCAGGgc      <  1:335678/93‑1 (MQ=255)
                                                                                   cGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTACTCAGGgc      <  2:2233392/93‑1 (MQ=255)
                                                                                   cGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTACTCAGGgc      <  1:1547164/93‑1 (MQ=255)
                                                                                    gTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTACTCAGGgcg     >  1:14296/1‑93 (MQ=255)
                                                                                    gTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGGGAGCGTAGGGATGATCATTACTCAGGgcg     >  2:2543540/1‑93 (MQ=255)
                                                                                      cTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTACTCAGGGCGCa   <  1:5431696/93‑1 (MQ=255)
                                                                                       tgtgCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTACTCAGGGCGCaa  >  1:2055108/1‑93 (MQ=255)
                                                                                          |                                                                                         
GTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTACTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTACTCAGGGCGCAA  >  NC_000913/772641‑772820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTACTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTACTCAGGGCGCAACCT  >  NC_000913/772638‑772823
                                                                                             |                                                                                            
TTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTG                                                                                              >  SRR4302533.2250018/1‑94 (MQ=60)
 TGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGC                                                                                             >  SRR4302533.3203661/1‑94 (MQ=60)
 TGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGC                                                                                             >  SRR4302533.2586023/1‑94 (MQ=60)
 TAGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGC                                                                                             >  SRR4302533.5978122/1‑94 (MQ=60)
  GGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCT                                                                                            >  SRR4302533.2040090/1‑94 (MQ=60)
    TAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTAC                                                                                          <  SRR4302533.3930293/94‑1 (MQ=60)
        TTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCG                                                                                      <  SRR4302533.6041523/94‑1 (MQ=60)
         TCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGG                                                                                     >  SRR4302533.199413/1‑94 (MQ=60)
          CGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGT                                                                                    <  SRR4302533.6286084/94‑1 (MQ=60)
           GTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTA                                                                                   <  SRR4302533.2196956/94‑1 (MQ=60)
            TTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAA                                                                                  >  SRR4302533.2826664/1‑94 (MQ=60)
             TCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAAC                                                                                 <  SRR4302533.4569808/94‑1 (MQ=60)
              CCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACT                                                                                >  SRR4302533.3615595/1‑94 (MQ=60)
               CGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTT                                                                               >  SRR4302533.5871548/1‑94 (MQ=60)
                 CCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCT                                                                             <  SRR4302533.5115947/94‑1 (MQ=60)
                 CCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCT                                                                             >  SRR4302533.4053078/1‑94 (MQ=60)
                   GCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTC                                                                           >  SRR4302533.1689824/1‑94 (MQ=60)
                   GCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTC                                                                           <  SRR4302533.241271/94‑1 (MQ=60)
                     TGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCA                                                                         >  SRR4302533.3953876/1‑94 (MQ=60)
                     TGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCA                                                                         <  SRR4302533.3699542/94‑1 (MQ=60)
                     TGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCA                                                                         <  SRR4302533.70697/94‑1 (MQ=60)
                     TGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCA                                                                         <  SRR4302533.3763939/94‑1 (MQ=60)
                     TGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCA                                                                         <  SRR4302533.5150397/94‑1 (MQ=60)
                      GGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAG                                                                        <  SRR4302533.3648627/94‑1 (MQ=60)
                       GTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGT                                                                       >  SRR4302533.403255/1‑94 (MQ=60)
                       GTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCCTCCAGT                                                                       >  SRR4302533.6186284/1‑94 (MQ=60)
                        TAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTT                                                                      <  SRR4302533.150125/94‑1 (MQ=60)
                        TAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTT                                                                      >  SRR4302533.3227185/1‑94 (MQ=60)
                        TAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTT                                                                      >  SRR4302533.374131/1‑94 (MQ=60)
                         AATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTG                                                                     <  SRR4302533.2754517/94‑1 (MQ=60)
                         AATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTG                                                                     <  SRR4302533.3208205/94‑1 (MQ=60)
                         AATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTG                                                                     >  SRR4302533.5560883/1‑94 (MQ=60)
                         AATTGGTGTAGCGTTCGGTAACCAGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTG                                                                     <  SRR4302533.746943/94‑1 (MQ=60)
                          ATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGC                                                                    >  SRR4302533.1537134/1‑94 (MQ=60)
                              GTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAA                                                                <  SRR4302533.4516063/94‑1 (MQ=60)
                               TGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAAC                                                               <  SRR4302533.6053930/94‑1 (MQ=60)
                                  AGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCG                                                            <  SRR4302533.4771751/94‑1 (MQ=60)
                                     GTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTC                                                         <  SRR4302533.2090583/94‑1 (MQ=60)
                                     GTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTC                                                         <  SRR4302533.4932073/94‑1 (MQ=60)
                                     GTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTC                                                         >  SRR4302533.3664772/1‑94 (MQ=60)
                                      TTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCG                                                        <  SRR4302533.3279466/94‑1 (MQ=60)
                                       TCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGG                                                       >  SRR4302533.2787668/1‑94 (MQ=60)
                                       TCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGG                                                       <  SRR4302533.2432742/94‑1 (MQ=60)
                                        CGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGC                                                      >  SRR4302533.4244892/1‑94 (MQ=60)
                                          GTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCT                                                    >  SRR4302533.5879199/1‑94 (MQ=60)
                                          GTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCT                                                    <  SRR4302533.4709846/94‑1 (MQ=60)
                                           TAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTG                                                   >  SRR4302533.2545573/1‑94 (MQ=60)
                                           TAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTG                                                   >  SRR4302533.4895609/1‑94 (MQ=60)
                                             ACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCT                                                 <  SRR4302533.1099171/94‑1 (MQ=60)
                                              CCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTG                                                >  SRR4302533.62843/1‑94 (MQ=60)
                                              CCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTG                                                <  SRR4302533.1575505/94‑1 (MQ=60)
                                               CTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGG                                               >  SRR4302533.3563801/1‑94 (MQ=60)
                                               CTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGG                                               >  SRR4302533.3972213/1‑94 (MQ=60)
                                               CTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGACTGCTGG                                               >  SRR4302533.628719/1‑94 (MQ=60)
                                               CTGTTGCAGGGCGGACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGG                                               >  SRR4302533.3424767/1‑94 (MQ=60)
                                                  TTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAG                                            <  SRR4302533.3798513/94‑1 (MQ=60)
                                                    GCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGC                                          >  SRR4302533.4433575/1‑94 (MQ=60)
                                                     CAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCG                                         >  SRR4302533.937877/1‑94 (MQ=60)
                                                      AGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGT                                        >  SRR4302533.1268154/1‑94 (MQ=60)
                                                      AGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGT                                        <  SRR4302533.3053153/94‑1 (MQ=60)
                                                       GGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTG                                       >  SRR4302533.3703649/1‑94 (MQ=60)
                                                        GGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGG                                      >  SRR4302533.2022527/1‑94 (MQ=60)
                                                         GCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGT                                     <  SRR4302533.3687207/94‑1 (MQ=60)
                                                         GCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGT                                     <  SRR4302533.930301/94‑1 (MQ=60)
                                                         GCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGG                                     >  SRR4302533.2712814/1‑94 (MQ=60)
                                                          CGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTG                                    <  SRR4302533.374150/94‑1 (MQ=60)
                                                          CGTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGCG                                    >  SRR4302533.1428373/1‑94 (MQ=60)
                                                           GTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGG                                   >  SRR4302533.2753132/1‑94 (MQ=60)
                                                           GTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGA                                   <  SRR4302533.4529890/94‑1 (MQ=60)
                                                           GTACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGA                                   <  SRR4302533.4737005/94‑1 (MQ=60)
                                                            TACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAG                                  <  SRR4302533.3821601/94‑1 (MQ=60)
                                                            TACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAG                                  >  SRR4302533.2579206/1‑94 (MQ=60)
                                                            TACCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAG                                  >  SRR4302533.3002464/1‑94 (MQ=60)
                                                              CCGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCG                                >  SRR4302533.3952271/1‑94 (MQ=60)
                                                              CCGCTGAACGTTGATGAAGAGCTGCGTCTGTGCTACACCGTTAACTTCTTCCAGTGGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCG                                <  SRR4302533.4850947/94‑1 (MQ=60)
                                                               CGTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGT                               >  SRR4302533.5701749/1‑94 (MQ=60)
                                                                GTTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTA                              >  SRR4302533.3386640/1‑94 (MQ=60)
                                                                CTTCAACGTTGATGAATATCTGCGTCTGTGCTACTCCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTA                              <  SRR4302533.2041125/94‑1 (MQ=60)
                                                                 TTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAG                             >  SRR4302533.4517932/1‑94 (MQ=60)
                                                                 TTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAG                             <  SRR4302533.5295856/94‑1 (MQ=60)
                                                                 TTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAG                             <  SRR4302533.6051326/94‑1 (MQ=60)
                                                                 TTCAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAG                             <  SRR4302533.5983726/94‑1 (MQ=60)
                                                                   CAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGGGTAGGG                           >  SRR4302533.5808034/1‑94 (MQ=60)
                                                                   CAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGG                           >  SRR4302533.38776/1‑94 (MQ=60)
                                                                   CAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGG                           >  SRR4302533.2152267/1‑94 (MQ=60)
                                                                   CAACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGG                           <  SRR4302533.3449657/94‑1 (MQ=60)
                                                                    AACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGA                          >  SRR4302533.1177100/1‑94 (MQ=60)
                                                                    AACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGA                          <  SRR4302533.1569592/94‑1 (MQ=60)
                                                                     ACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGAT                         >  SRR4302533.1339450/1‑94 (MQ=60)
                                                                     ACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGAT                         >  SRR4302533.2643053/1‑94 (MQ=60)
                                                                     ACGTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGGGAGCGTAGGGGT                         >  SRR4302533.5301540/1‑94 (MQ=60)
                                                                       GTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGA                       <  SRR4302533.1761316/94‑1 (MQ=60)
                                                                       GTTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGA                       >  SRR4302533.2562679/1‑94 (MQ=60)
                                                                        TTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGAT                      >  SRR4302533.3269365/1‑94 (MQ=60)
                                                                        TTGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGAGGTGGGCGTACGGATGGT                      >  SRR4302533.5414077/1‑94 (MQ=60)
                                                                         TGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATC                     >  SRR4302533.1825977/1‑94 (MQ=60)
                                                                         TGATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATC                     >  SRR4302533.3293673/1‑94 (MQ=60)
                                                                           ATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCAT                   >  SRR4302533.1588703/1‑94 (MQ=60)
                                                                           ATGAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCAT                   >  SRR4302533.1331873/1‑94 (MQ=60)
                                                                             GAATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTA                 <  SRR4302533.954141/94‑1 (MQ=60)
                                                                              AATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTAC                >  SRR4302533.4638195/1‑94 (MQ=60)
                                                                              AATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTAC                >  SRR4302533.5169946/1‑94 (MQ=60)
                                                                              AATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTAC                <  SRR4302533.4886045/94‑1 (MQ=60)
                                                                               ATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTACT               <  SRR4302533.5845658/94‑1 (MQ=60)
                                                                               ATATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTACT               >  SRR4302533.850617/1‑94 (MQ=60)
                                                                                TATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGGTGATCATTACTC              >  SRR4302533.6090390/1‑94 (MQ=60)
                                                                                 ATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAGCCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTACTCA             <  SRR4302533.3940520/94‑1 (MQ=60)
                                                                                 ATCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTACTCA             >  SRR4302533.727241/1‑94 (MQ=60)
                                                                                  TCTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTACTCAG            <  SRR4302533.4568135/94‑1 (MQ=60)
                                                                                   CTGCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTACTCAGG           >  SRR4302533.1452302/1‑94 (MQ=60)
                                                                                     GCGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTACTCAGGGC         <  SRR4302533.3684339/94‑1 (MQ=60)
                                                                                      TGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTACTCAGGGCG        <  SRR4302533.4950986/94‑1 (MQ=60)
                                                                                      CGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTACTCAGGGCG        <  SRR4302533.1556488/94‑1 (MQ=60)
                                                                                      CGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTACTCAGGGCG        >  SRR4302533.14400/1‑94 (MQ=60)
                                                                                      CGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTACTCAGGGCG        <  SRR4302533.2574885/94‑1 (MQ=60)
                                                                                      CGTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTACTCAGGGCG        <  SRR4302533.337730/94‑1 (MQ=60)
                                                                                       GTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTACTCAGGGCGC       <  SRR4302533.3991133/94‑1 (MQ=60)
                                                                                       GTCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTACTCAGGGCGC       >  SRR4302533.1326491/1‑94 (MQ=60)
                                                                                        TCTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTACTCAGGGCGCA      >  SRR4302533.1642310/1‑94 (MQ=60)
                                                                                         CTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTACTCAGGGCGCAA     >  SRR4302533.2067784/1‑94 (MQ=60)
                                                                                         CTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTACTCAGGGCGCAA     >  SRR4302533.2524495/1‑94 (MQ=60)
                                                                                         CTGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTACTCAGGGCGCAA     <  SRR4302533.5464405/94‑1 (MQ=60)
                                                                                          TGTGCTACACCGGTAACTTCTTCCAGTTGCTTATCCCGTTCGGCCTGCTGGCAGGCGCGGTGAGAGTAGGGATGATCATTATTCAGGGCGCAAC    >  SRR4302533.3763371/1‑94 (MQ=60)
                                                                                          TGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTACTCAGGGCGCCAC    >  SRR4302533.5143048/1‑94 (MQ=60)
                                                                                          TGTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTACTCAGGGCGCAAC    <  SRR4302533.5135522/94‑1 (MQ=60)
                                                                                           GTGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTACTCAGGGCGCAACC   >  SRR4302533.3765206/1‑94 (MQ=60)
                                                                                            TGCTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTACTCAGGGCGCAACCT  <  SRR4302533.3478418/94‑1 (MQ=60)
                                                                                             |                                                                                            
TTGGTAGCTTCGTTCCGCCGCTGGTAATTGGTGTAGCGTTCGGTAACCTGTTGCAGGGCGTACCGTTCAACGTTGATGAATATCTGCGTCTGTACTACACCGGTAACTTCTTCCAGTTGCTTAACCCGTTCGGCCTGCTGGCAGGCGTGGTGAGCGTAGGGATGATCATTACTCAGGGCGCAACCT  >  NC_000913/772638‑772823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑