Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	888,315	C→T	G243D (GGC→GAC)	b0847 ←	putative transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	888,315	0	C	T	99.6%	949.7 / NA	259	G243D (GGC→GAC)	b0847	putative transport protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (132/126); minor base G (0/1); total (132/127)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 267 total aligned reads displayed.

ACCAACGCTATTTCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGATCACC  >  NC_000913/888225‑888404

 ccAACGCTATTTCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCgg                                                                                        <  1:4667074/93‑1 (MQ=255)

    aCGCTATTTCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGttt                                                                                     >  2:181736/1‑93 (MQ=255)

         aTTTCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGa                                                                                >  1:2179086/1‑93 (MQ=255)

          tttCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGATTACGTTCAATGTAGCAGTCGGTTTGTCGAt                                                                               <  2:4687773/93‑1 (MQ=255)

          tttCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGAt                                                                               >  2:3819022/1‑93 (MQ=255)

          tttCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGAt                                                                               <  1:5486518/93‑1 (MQ=255)

          tttCATCGCACATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGGCGGTTTGTCGAt                                                                               <  2:1046393/93‑1 (MQ=255)

           ttCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATa                                                                              <  1:1102772/93‑1 (MQ=255)

           ttCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATa                                                                              >  2:5370011/1‑93 (MQ=255)

            tCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATaa                                                                             >  1:2686272/1‑93 (MQ=255)

            tCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATaa                                                                             >  1:5857867/1‑93 (MQ=255)

              aTCGCCCGTTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTAGGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAAt                                                                           <  1:303853/93‑1 (MQ=255)

              aTCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAAt                                                                           >  1:5133939/1‑93 (MQ=255)

              aTCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAAt                                                                           <  1:4982770/93‑1 (MQ=255)

               tCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATa                                                                          <  2:3478990/93‑1 (MQ=255)

               tCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATa                                                                          >  2:2642630/1‑93 (MQ=255)

                cGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATAc                                                                         <  1:3998662/93‑1 (MQ=255)

                 gCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATAcc                                                                        >  1:5425428/1‑93 (MQ=255)

                 gCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATAcc                                                                        >  1:1774106/1‑93 (MQ=255)

                 gCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGGTTGTCGATAAATAcc                                                                        >  2:5933853/1‑93 (MQ=255)

                  cccATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATAccc                                                                       >  2:3024895/1‑93 (MQ=255)

                  cccATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATAccc                                                                       >  1:5544914/1‑93 (MQ=255)

                   ccATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCa                                                                      >  1:4427711/1‑93 (MQ=255)

                    cATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAg                                                                     <  2:2365659/93‑1 (MQ=255)

                    cATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAg                                                                     <  2:4067354/93‑1 (MQ=255)

                    cATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAg                                                                     >  2:1273418/1‑93 (MQ=255)

                    cATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAg                                                                     <  1:277830/93‑1 (MQ=255)

                    cATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAg                                                                     <  1:2494102/93‑1 (MQ=255)

                     aTTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGt                                                                    <  1:3101977/93‑1 (MQ=255)

                     aTTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGt                                                                    >  2:3704/1‑93 (MQ=255)

                     aTTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAACACCCAGt                                                                    <  2:6196337/93‑1 (MQ=255)

                       ttGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTc                                                                  <  2:232438/93‑1 (MQ=255)

                        tGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCa                                                                 <  1:5274532/93‑1 (MQ=255)

                         gTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCAc                                                                <  2:5490997/93‑1 (MQ=255)

                         gTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCAc                                                                <  2:337487/93‑1 (MQ=255)

                           aGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGc                                                              >  2:5762069/1‑93 (MQ=255)

                           aGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGc                                                              <  1:3973939/93‑1 (MQ=255)

                            gCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCa                                                             >  1:3319984/1‑93 (MQ=255)

                            gCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCa                                                             <  2:2578681/93‑1 (MQ=255)

                                ggCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTTGATAAATACCCAGTTCACGCAGAtt                                                         <  2:2924301/93‑1 (MQ=255)

                                 gCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGAttt                                                        >  2:4266424/1‑93 (MQ=255)

                                   aTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGAttttt                                                      >  1:613344/1‑93 (MQ=255)

                                    tCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTg                                                     >  1:1520877/1‑93 (MQ=255)

                                    tCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTg                                                     >  1:2065035/1‑93 (MQ=255)

                                     cACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGc                                                    <  2:3062002/93‑1 (MQ=255)

                                      aCCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAATTCACGCAGATTTTTGcc                                                   >  1:127035/1‑93 (MQ=255)

                                       ccGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCg                                                  >  1:790431/1‑93 (MQ=255)

                                        cGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGt                                                 >  1:5666558/1‑93 (MQ=255)

                                         gTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTc                                                >  2:4322987/1‑93 (MQ=255)

                                           cTGGATTTGCCAGAATCCCATTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCgg                                              <  2:3868096/93‑1 (MQ=255)

                                           cTGGATGTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCgg                                              <  2:2239003/93‑1 (MQ=255)

                                            tGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGt                                             >  2:5885205/1‑93 (MQ=255)

                                             ggATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTc                                            <  2:4865211/93‑1 (MQ=255)

                                               aTTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCa                                          >  1:5731338/1‑93 (MQ=255)

                                                tttGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAg                                         <  2:5064034/93‑1 (MQ=255)

                                                tttGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAg                                         >  2:5810614/1‑93 (MQ=255)

                                                tttGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAg                                         <  2:1140469/93‑1 (MQ=255)

                                                tttGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAg                                         >  1:5093725/1‑93 (MQ=255)

                                                   gCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGcc                                      >  2:2295458/1‑93 (MQ=255)

                                                   gCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGcc                                      <  1:3791656/93‑1 (MQ=255)

                                                    ccAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGcca                                     >  1:1030055/1‑93 (MQ=255)

                                                     cAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGccac                                    >  1:985466/1‑93 (MQ=255)

                                                       gAATCTCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGccacca                                  <  1:380523/93‑1 (MQ=255)

                                                       gAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGccacca                                  <  2:3182260/93‑1 (MQ=255)

                                                       gAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGccacca                                  <  1:4598604/93‑1 (MQ=255)

                                                       gAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGccacca                                  <  1:5638776/93‑1 (MQ=255)

                                                       gAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGccacca                                  <  1:5795865/93‑1 (MQ=255)

                                                       gAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGccacca                                  <  1:944679/93‑1 (MQ=255)

                                                       gAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTCGCCGTCGGTCCAGGccacca                                  <  2:2679041/93‑1 (MQ=255)

                                                         aTCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGt                                <  2:1553531/93‑1 (MQ=255)

                                                         aTCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGt                                >  2:75729/1‑93 (MQ=255)

                                                           cccGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTc                              <  1:2949859/93‑1 (MQ=255)

                                                             cGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCg                            <  2:3286954/93‑1 (MQ=255)

                                                              gTTACGTCGAATACGTTCAATGTGGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCgg                           >  1:5995136/1‑93 (MQ=255)

                                                              gTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCgg                           >  1:3389161/1‑93 (MQ=255)

                                                              gTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCgg                           <  2:5441963/93‑1 (MQ=255)

                                                               ttACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCggg                          >  1:4292107/1‑93 (MQ=255)

                                                                tACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGc                         <  2:916930/93‑1 (MQ=255)

                                                                tACGTAGGATACCTTCAATGGAGCAGTAGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGTTCCAGGCCACCAGTTCCGGGc                         <  1:1966860/93‑1 (MQ=255)

                                                                 aCGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGcc                        >  2:3070339/1‑93 (MQ=255)

                                                                   gTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGa                      >  2:5212554/1‑93 (MQ=255)

                                                                   gTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGa                      >  2:709457/1‑93 (MQ=255)

                                                                    tCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGAc                     >  1:1542838/1‑93 (MQ=255)

                                                                    tCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGAc                     <  1:3637212/93‑1 (MQ=255)

                                                                     cGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGAcg                    >  2:2991074/1‑93 (MQ=255)

                                                                        aTACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGa                 >  2:5144032/1‑93 (MQ=255)

                                                                         tACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGAt                >  2:4468047/1‑93 (MQ=255)

                                                                         tACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGAt                <  2:2815798/93‑1 (MQ=255)

                                                                         tACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGAt                <  1:5231690/93‑1 (MQ=255)

                                                                          aCGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATa               <  1:2352610/93‑1 (MQ=255)

                                                                          aCGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATa               <  1:6257709/93‑1 (MQ=255)

                                                                          aCGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATa               <  1:1682632/93‑1 (MQ=255)

                                                                          aCGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATa               <  2:562783/93‑1 (MQ=255)

                                                                            gTTCAATGTAGCAGTCGGTTCGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAgg             <  1:4205070/93‑1 (MQ=255)

                                                                               cAATGTAGCAGTCGTTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGgcgc          <  2:1956083/93‑1 (MQ=255)

                                                                                aaTGTAGCAGTCGGTTTGTTGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGcgcg         >  2:6121033/1‑93 (MQ=255)

                                                                                  tGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGa       >  1:4782385/1‑93 (MQ=255)

                                                                                  tGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGa       <  2:6261953/93‑1 (MQ=255)

                                                                                   gTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGAt      <  1:3229573/93‑1 (MQ=255)

                                                                                    tAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGATc     >  2:3540913/1‑93 (MQ=255)

ACCAACGCTATTTCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGATCACC  >  NC_000913/888225‑888404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGATCACCGGTA  >  NC_000913/888223‑888408

CTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTC                                                                                              >  SRR4302533.4777747/1‑94 (MQ=60)

CTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTC                                                                                              >  SRR4302533.1223408/1‑94 (MQ=60)

CTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTC                                                                                              >  SRR4302533.2638393/1‑94 (MQ=60)

CTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTC                                                                                              >  SRR4302533.4228416/1‑94 (MQ=60)

CTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCATCACCGTCGGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTC                                                                                              >  SRR4302533.4110021/1‑94 (MQ=60)

  ACCAACGCTATTTCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGG                                                                                            <  SRR4302533.1707797/94‑1 (MQ=60)

   CCAACGCTATTTCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGT                                                                                           <  SRR4302533.4695350/94‑1 (MQ=60)

    CAACGCTATTTCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTT                                                                                          <  SRR4302533.559834/94‑1 (MQ=60)

    CAACGCTATTTCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTT                                                                                          >  SRR4302533.6230465/1‑94 (MQ=60)

     AACGCTATTTCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCGAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTT                                                                                         <  SRR4302533.2447412/94‑1 (MQ=60)

     AACGCTATTTCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTT                                                                                         <  SRR4302533.3060494/94‑1 (MQ=60)

      ACGCTATTTCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTG                                                                                        <  SRR4302533.4911672/94‑1 (MQ=60)

      ACGCTATTTCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTG                                                                                        >  SRR4302533.3932547/1‑94 (MQ=60)

         CTATTTCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCG                                                                                     >  SRR4302533.1768723/1‑94 (MQ=60)

          TATTTCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGA                                                                                    >  SRR4302533.2192520/1‑94 (MQ=60)

          TATTTCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGA                                                                                    >  SRR4302533.1136348/1‑94 (MQ=60)

           ATTTCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGAT                                                                                   >  SRR4302533.4719859/1‑94 (MQ=60)

           ATTTCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGAT                                                                                   >  SRR4302533.3956454/1‑94 (MQ=60)

           ATTTCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGAT                                                                                   >  SRR4302533.4001546/1‑94 (MQ=60)

            TTTCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATA                                                                                  <  SRR4302533.5519590/94‑1 (MQ=60)

            TTTCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATA                                                                                  <  SRR4302533.3789385/94‑1 (MQ=60)

             TTCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAA                                                                                 <  SRR4302533.1109302/94‑1 (MQ=60)

             TTCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAA                                                                                 >  SRR4302533.2702740/1‑94 (MQ=60)

             TTCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAA                                                                                 >  SRR4302533.5893279/1‑94 (MQ=60)

              TCATCGCCCATTTGTAGCACGGCATCCCCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAA                                                                                <  SRR4302533.5164790/94‑1 (MQ=60)

              TCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAA                                                                                <  SRR4302533.601450/94‑1 (MQ=60)

               CATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAAT                                                                               >  SRR4302533.5164953/1‑94 (MQ=60)

               CATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAAT                                                                               <  SRR4302533.2188328/94‑1 (MQ=60)

                ATCGCCCGTTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTAGGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATA                                                                              <  SRR4302533.305708/94‑1 (MQ=60)

                ATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATA                                                                              <  SRR4302533.5012982/94‑1 (MQ=60)

                 TCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATAC                                                                             <  SRR4302533.5796337/94‑1 (MQ=60)

                  CGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACC                                                                            >  SRR4302533.5458102/1‑94 (MQ=60)

                  CGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACC                                                                            >  SRR4302533.4278611/1‑94 (MQ=60)

                  CGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACC                                                                            >  SRR4302533.1784910/1‑94 (MQ=60)

                  CGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACC                                                                            <  SRR4302533.4023005/94‑1 (MQ=60)

                   GCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCC                                                                           >  SRR4302533.5578405/1‑94 (MQ=60)

                    CCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCA                                                                          >  SRR4302533.4454475/1‑94 (MQ=60)

                    CCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCA                                                                          >  SRR4302533.5298478/1‑94 (MQ=60)

                     CCATTTGTAGCACGACATCACCTTCTGGATTTGCCAGAATCCCGGTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAG                                                                         <  SRR4302533.1435080/94‑1 (MQ=60)

                      CATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGT                                                                        <  SRR4302533.2509488/94‑1 (MQ=60)

                      CATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGT                                                                        <  SRR4302533.279525/94‑1 (MQ=60)

                      CATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGT                                                                        >  SRR4302533.3431875/1‑94 (MQ=60)

                       ATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCGAGTT                                                                       >  SRR4302533.2701561/1‑94 (MQ=60)

                       ATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTT                                                                       >  SRR4302533.2375744/1‑94 (MQ=60)

                       ATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTT                                                                       <  SRR4302533.3121048/94‑1 (MQ=60)

                        TTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTC                                                                      <  SRR4302533.3678172/94‑1 (MQ=60)

                         TTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCA                                                                     <  SRR4302533.2033621/94‑1 (MQ=60)

                          TGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCAC                                                                    <  SRR4302533.5306158/94‑1 (MQ=60)

                          TGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCAC                                                                    <  SRR4302533.3270116/94‑1 (MQ=60)

                             AGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCA                                                                 >  SRR4302533.3340491/1‑94 (MQ=60)

                             AGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCA                                                                 <  SRR4302533.3998136/94‑1 (MQ=60)

                               CACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGA                                                               >  SRR4302533.5705734/1‑94 (MQ=60)

                                ACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGAT                                                              >  SRR4302533.4430977/1‑94 (MQ=60)

                                ACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGAT                                                              <  SRR4302533.1546401/94‑1 (MQ=60)

                                    CATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTT                                                          >  SRR4302533.616949/1‑94 (MQ=60)

                                     ATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTG                                                         >  SRR4302533.5586431/1‑94 (MQ=60)

                                     ATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTG                                                         >  SRR4302533.2077763/1‑94 (MQ=60)

                                     ATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTG                                                         >  SRR4302533.1530033/1‑94 (MQ=60)

                                       CACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCC                                                       >  SRR4302533.4012515/1‑94 (MQ=60)

                                       CACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAATTCACGCAGATTTTTGCC                                                       >  SRR4302533.127831/1‑94 (MQ=60)

                                        ACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCG                                                      >  SRR4302533.795085/1‑94 (MQ=60)

                                        ACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCG                                                      >  SRR4302533.5872922/1‑94 (MQ=60)

                                         CCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGT                                                     >  SRR4302533.569116/1‑94 (MQ=60)

                                         CCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGT                                                     >  SRR4302533.5700789/1‑94 (MQ=60)

                                           GTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCCATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGTCGTCG                                                   >  SRR4302533.3218318/1‑94 (MQ=60)

                                           GTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCG                                                   >  SRR4302533.3181004/1‑94 (MQ=60)

                                           GTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCG                                                   >  SRR4302533.6163444/1‑94 (MQ=60)

                                           GTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCG                                                   <  SRR4302533.5292966/94‑1 (MQ=60)

                                            TCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGG                                                  >  SRR4302533.5084738/1‑94 (MQ=60)

                                            TCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGG                                                  >  SRR4302533.1060779/1‑94 (MQ=60)

                                             CTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGT                                                 <  SRR4302533.1914321/94‑1 (MQ=60)

                                             CTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGATCACGCAGATTTTTtcattcggt                                                 >  SRR4302533.3487046/1‑85 (MQ=60)

                                              TGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTC                                                <  SRR4302533.123839/94‑1 (MQ=60)

                                               GGACTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCC                                               >  SRR4302533.1593924/1‑94 (MQ=60)

                                                GATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCA                                              >  SRR4302533.5765941/1‑94 (MQ=60)

                                                GATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCA                                              <  SRR4302533.572330/94‑1 (MQ=60)

                                                 ATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAG                                             <  SRR4302533.5797128/94‑1 (MQ=60)

                                                 ATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAG                                             >  SRR4302533.5124496/1‑94 (MQ=60)

                                                     GCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCA                                         >  SRR4302533.1036109/1‑94 (MQ=60)

                                                     GCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCA                                         <  SRR4302533.3814869/94‑1 (MQ=60)

                                                      CCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCAC                                        >  SRR4302533.991236/1‑94 (MQ=60)

                                                       CAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACC                                       >  SRR4302533.556794/1‑94 (MQ=60)

                                                        AGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCA                                      >  SRR4302533.4402213/1‑94 (MQ=60)

                                                         GAATCTCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAG                                     <  SRR4302533.382814/94‑1 (MQ=60)

                                                         GAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAG                                     <  SRR4302533.4626465/94‑1 (MQ=60)

                                                         GAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAG                                     <  SRR4302533.5830882/94‑1 (MQ=60)

                                                         GAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAG                                     <  SRR4302533.5672866/94‑1 (MQ=60)

                                                         GAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAG                                     <  SRR4302533.950235/94‑1 (MQ=60)

                                                         GAATCCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAG                                     <  SRR4302533.4782424/94‑1 (MQ=60)

                                                             CCCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCC                                 <  SRR4302533.2967899/94‑1 (MQ=60)

                                                              CCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCG                                <  SRR4302533.2112872/94‑1 (MQ=60)

                                                              CCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCG                                >  SRR4302533.2976507/1‑94 (MQ=60)

                                                              CCGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCG                                >  SRR4302533.4623741/1‑94 (MQ=60)

                                                               CGTTACGTCGAATACGTTCAATGTGGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGG                               >  SRR4302533.6031444/1‑94 (MQ=60)

                                                               CGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGG                               <  SRR4302533.3301306/94‑1 (MQ=60)

                                                               CGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGG                               <  SRR4302533.4208720/94‑1 (MQ=60)

                                                               CGTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGG                               >  SRR4302533.3410090/1‑94 (MQ=60)

                                                                GTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGG                              <  SRR4302533.3814943/94‑1 (MQ=60)

                                                                GTTACGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGG                              >  SRR4302533.4317919/1‑94 (MQ=60)

                                                                  TACGTAGGATACCTTCAATGGAGCAGTAGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGTTCCAGGCCACCAGTTCCGGGCC                            <  SRR4302533.1978880/94‑1 (MQ=60)

                                                                    CGTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGA                          >  SRR4302533.689431/1‑94 (MQ=60)

                                                                     GTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGAC                         >  SRR4302533.1552130/1‑94 (MQ=60)

                                                                     GTCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGAC                         <  SRR4302533.6037942/94‑1 (MQ=60)

                                                                      TCGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACG                        <  SRR4302533.3659575/94‑1 (MQ=60)

                                                                       CGAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGC                       <  SRR4302533.5470773/94‑1 (MQ=60)

                                                                        GAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCG                      >  SRR4302533.2211029/1‑94 (MQ=60)

                                                                        GAATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCG                      >  SRR4302533.5407833/1‑94 (MQ=60)

                                                                          ATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGAT                    >  SRR4302533.5398396/1‑94 (MQ=60)

                                                                          ATACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGAT                    <  SRR4302533.339995/94‑1 (MQ=60)

                                                                           TACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATA                   <  SRR4302533.5263156/94‑1 (MQ=60)

                                                                            ACGTTCAATGTAGCAGTCTGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAG                  <  SRR4302533.1482787/94‑1 (MQ=60)

                                                                            ACGTTCAATGTAGCAGTCGGTTTGTCGATAAATTCCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAG                  <  SRR4302533.1622134/94‑1 (MQ=60)

                                                                            ACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAG                  <  SRR4302533.4665432/94‑1 (MQ=60)

                                                                            ACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAG                  <  SRR4302533.1692824/94‑1 (MQ=60)

                                                                            ACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAG                  <  SRR4302533.6295131/94‑1 (MQ=60)

                                                                            ACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAG                  <  SRR4302533.2367162/94‑1 (MQ=60)

                                                                            ACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAG                  <  SRR4302533.2543697/94‑1 (MQ=60)

                                                                            ACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAG                  <  SRR4302533.2809875/94‑1 (MQ=60)

                                                                            ACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAG                  <  SRR4302533.918092/94‑1 (MQ=60)

                                                                            ACGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAG                  >  SRR4302533.2978130/1‑94 (MQ=60)

                                                                            ACGTTCAATGTAGCAGTCGGTTTGTCGAAAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAG                  <  SRR4302533.2809876/94‑1 (MQ=60)

                                                                             CGTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGG                 >  SRR4302533.517420/1‑94 (MQ=60)

                                                                              GTTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGG                >  SRR4302533.4282580/1‑94 (MQ=60)

                                                                              GTTCAATGTAGCAGTCGGTTCGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGC                <  SRR4302533.4230508/94‑1 (MQ=60)

                                                                               TTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCG               >  SRR4302533.662508/1‑94 (MQ=60)

                                                                               TTCAATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCG               >  SRR4302533.880577/1‑94 (MQ=60)

                                                                                  AATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGG            >  SRR4302533.2039248/1‑94 (MQ=60)

                                                                                   ATGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGA           >  SRR4302533.4811360/1‑94 (MQ=60)

                                                                                    TGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGAT          <  SRR4302533.2256534/94‑1 (MQ=60)

                                                                                    TGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGAT          <  SRR4302533.6028654/94‑1 (MQ=60)

                                                                                    TGTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGAT          <  SRR4302533.5156970/94‑1 (MQ=60)

                                                                                     GTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGATC         <  SRR4302533.5571033/94‑1 (MQ=60)

                                                                                     GTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGATC         <  SRR4302533.2761528/94‑1 (MQ=60)

                                                                                     GTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGATC         <  SRR4302533.3249483/94‑1 (MQ=60)

                                                                                     GTAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGATC         <  SRR4302533.954744/94‑1 (MQ=60)

                                                                                      TAGCAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGATCA        <  SRR4302533.4650573/94‑1 (MQ=60)

                                                                                         CAGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGATCACCG     <  SRR4302533.5029763/94‑1 (MQ=60)

                                                                                          AGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGATCACCGG    >  SRR4302533.1631192/1‑94 (MQ=60)

                                                                                          AGTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGATCACCGG    >  SRR4302533.2351226/1‑94 (MQ=60)

                                                                                           GTCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGATCACCGGT   >  SRR4302533.3830475/1‑94 (MQ=60)

                                                                                            TCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGATCACCGGTA  <  SRR4302533.3823991/94‑1 (MQ=60)

                                                                                            TCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGATCACCGGTA  <  SRR4302533.4860026/94‑1 (MQ=60)

                                                                                            TCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGATCACCGGTA  >  SRR4302533.3374274/1‑94 (MQ=60)

CTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGATCACCGGTA  >  NC_000913/888223‑888408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 5 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`