Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	922,398	T→C	V88A (GTG→GCG)	yljA →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	922,398	0	T	C	99.5%	652.4 / NA	201	V88A (GTG→GCG)	yljA	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (85/115); minor base G (0/1); total (85/116)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 210 total aligned reads displayed.

CAACGCAATTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGTGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGGGGAGGTGCCTA  >  NC_000913/922308‑922487

 aaCGCAATTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGaa                                                                                        >  1:2813783/1‑93 (MQ=255)

  aCGCAATTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAAc                                                                                       >  1:6198057/1‑93 (MQ=255)

  aCGCAATTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAAc                                                                                       >  1:5698559/1‑93 (MQ=255)

   cGCAATTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACa                                                                                      >  2:3268738/1‑93 (MQ=255)

    gCAATTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACaa                                                                                     <  1:2261071/93‑1 (MQ=255)

      aaTTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGt                                                                                   <  1:1179751/93‑1 (MQ=255)

      aaTTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGt                                                                                   <  1:1866503/93‑1 (MQ=255)

       aTTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTa                                                                                  <  2:2465446/93‑1 (MQ=255)

        ttGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTAc                                                                                 <  2:5350133/93‑1 (MQ=255)

        ttGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTAc                                                                                 <  1:5176388/93‑1 (MQ=255)

         tGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTAcg                                                                                >  1:5724350/1‑93 (MQ=255)

         tGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTAcg                                                                                >  2:1979321/1‑93 (MQ=255)

          gATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTAcgc                                                                               >  1:6258103/1‑93 (MQ=255)

          gATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTAcgc                                                                               <  2:3634889/93‑1 (MQ=255)

          gATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTAcgc                                                                               <  2:3751524/93‑1 (MQ=255)

           aTGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTAcgcg                                                                              >  1:5589178/1‑93 (MQ=255)

            tGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGa                                                                             >  1:3564709/1‑93 (MQ=255)

            tGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGa                                                                             >  2:5001382/1‑93 (MQ=255)

            agCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGa                                                                             <  1:4940042/92‑1 (MQ=255)

             gCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAg                                                                            <  1:4279730/93‑1 (MQ=255)

             gCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAg                                                                            >  1:923903/1‑93 (MQ=255)

              cTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAgg                                                                           <  1:4750864/93‑1 (MQ=255)

                cGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGga                                                                         <  2:3996068/93‑1 (MQ=255)

                cGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGga                                                                         <  2:2564390/93‑1 (MQ=255)

                 gCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGgag                                                                        <  2:1937645/93‑1 (MQ=255)

                  cTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGgaga                                                                       >  2:5375067/1‑93 (MQ=255)

                   tGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGaa                                                                      >  1:5401330/1‑93 (MQ=255)

                        aCTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGc                                                                 >  1:3143629/1‑93 (MQ=255)

                        aCTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGc                                                                 <  1:4972983/93‑1 (MQ=255)

                        aCTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGc                                                                 <  1:6026435/93‑1 (MQ=255)

                         cTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCa                                                                >  2:4812880/1‑93 (MQ=255)

                          tACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCAt                                                               <  2:3304955/93‑1 (MQ=255)

                          tACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCAt                                                               <  1:873596/93‑1 (MQ=255)

                           aCCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATc                                                              >  1:3006894/1‑93 (MQ=255)

                             cAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCa                                                            >  2:2546796/1‑93 (MQ=255)

                             cAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCa                                                            >  2:580213/1‑93 (MQ=255)

                             cAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCa                                                            >  2:5804294/1‑93 (MQ=255)

                               ggggAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCAtt                                                          >  2:2314010/1‑93 (MQ=255)

                               ggggAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCAtt                                                          >  1:2511337/1‑93 (MQ=255)

                               ggggAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCAtg                                                          >  1:2166924/1‑92 (MQ=255)

                               ggggAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGCGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCAtt                                                          >  1:1097772/1‑93 (MQ=255)

                                gggAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTg                                                         <  1:2445228/93‑1 (MQ=255)

                                 ggAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGc                                                        <  1:6031401/93‑1 (MQ=255)

                                  gAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCt                                                       >  2:668430/1‑93 (MQ=255)

                                    aGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCtgt                                                     <  2:5611964/93‑1 (MQ=255)

                                     ggCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCtgtg                                                    >  2:114592/1‑93 (MQ=255)

                                     ggCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCtgtg                                                    <  1:999504/93‑1 (MQ=255)

                                       ccATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCTAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTa                                                  >  1:2868954/1‑93 (MQ=255)

                                        cATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTAc                                                 >  2:2675253/1‑93 (MQ=255)

                                        cATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTAc                                                 <  2:464796/93‑1 (MQ=255)

                                         aTTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACg                                                >  1:2320236/1‑93 (MQ=255)

                                         aTTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACg                                                >  2:37973/1‑93 (MQ=255)

                                          tttGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGc                                               >  2:4609965/1‑93 (MQ=255)

                                          tttGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGc                                               <  2:2769693/93‑1 (MQ=255)

                                          tttGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGc                                               >  2:1342465/1‑93 (MQ=255)

                                           ttGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCt                                              >  1:1693498/1‑93 (MQ=255)

                                           ttGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCt                                              >  1:3299964/1‑93 (MQ=255)

                                            tGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTa                                             <  1:2984341/93‑1 (MQ=255)

                                              cGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGa                                           <  2:3890759/93‑1 (MQ=255)

                                               ggAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGaa                                          >  1:3246955/1‑93 (MQ=255)

                                                 aGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGaaaa                                        >  1:5516303/1‑93 (MQ=255)

                                                 aGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGaaaa                                        <  2:1088746/93‑1 (MQ=255)

                                                    cTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGc                                     >  1:1548407/1‑93 (MQ=255)

                                                     tttACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCGTTGCTGTGTACGCTAGAAAAAGcc                                    <  2:6072662/93‑1 (MQ=255)

                                                     tttACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGcc                                    <  2:5929117/93‑1 (MQ=255)

                                                       tACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTg                                  <  2:735283/93‑1 (MQ=255)

                                                       tACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTg                                  <  1:592312/93‑1 (MQ=255)

                                                         ccgccgAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGaa                                <  1:508927/93‑1 (MQ=255)

                                                         ccgccgAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGaa                                <  2:4386502/93‑1 (MQ=255)

                                                         ccgccgAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGaa                                <  1:4604759/93‑1 (MQ=255)

                                                         ccgccgAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGaa                                >  1:4413866/1‑93 (MQ=255)

                                                            ccgAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGc                             >  1:4996018/1‑93 (MQ=255)

                                                             cgAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCa                            >  2:16262/1‑93 (MQ=255)

                                                             cgAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCa                            <  1:3726094/93‑1 (MQ=255)

                                                             cgAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCa                            >  1:4156012/1‑93 (MQ=255)

                                                              gAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAg                           <  1:2545566/93‑1 (MQ=255)

                                                                 gTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCa                        <  2:279652/93‑1 (MQ=255)

                                                                  ttGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCAt                       >  2:2835486/1‑93 (MQ=255)

                                                                  ttGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCAt                       <  1:2270790/93‑1 (MQ=255)

                                                                   tGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATa                      <  1:5852661/93‑1 (MQ=255)

                                                                       gAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATaaaaa                  <  2:3188905/93‑1 (MQ=255)

                                                                        aaaCCAAAGTGGCGATGGCGAACAAGTACGCGATGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAAt                 <  1:2233624/93‑1 (MQ=255)

                                                                        aaaCCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAAt                 >  1:4008568/1‑93 (MQ=255)

                                                                           ccAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTgg              <  1:389719/93‑1 (MQ=255)

                                                                            cAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTggg             <  2:5548917/93‑1 (MQ=255)

                                                                            cAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTggg             >  1:4867109/1‑93 (MQ=255)

                                                                             aaaGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTgggg            <  2:2086018/93‑1 (MQ=255)

                                                                             aaaGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTgggg            <  1:4449683/93‑1 (MQ=255)

                                                                             aaaGTGGCGACGACGAACAAGTACGCGAGCGACAATGAGCATCCATTGCTGTGTACGCTAAAAAAACCCTGAATGCAGGCATAAAAATTgggc            <  2:4586794/93‑2 (MQ=255)

                                                                              aaGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTggggg           <  1:753779/93‑1 (MQ=255)

                                                                               aGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGGGGa          >  1:6124054/1‑93 (MQ=255)

                                                                               aGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGGGGa          <  2:2222151/93‑1 (MQ=255)

                                                                                gTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGGGGAg         <  2:3339599/93‑1 (MQ=255)

                                                                                gTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGGGGAg         <  2:1872379/93‑1 (MQ=255)

                                                                                 tggcgatggcgaACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGGGGAgg        >  1:4680432/1‑93 (MQ=255)

                                                                                  ggCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGGGGAggg       >  1:5736026/1‑92 (MQ=255)

                                                                                  ggCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGGGGAGGt       <  2:3423081/93‑1 (MQ=255)

                                                                                     gATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGGGGAGGGGcc    >  2:2962168/1‑93 (MQ=255)

                                                                                      aTGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGGGGAGGTGCCt   <  1:6025463/93‑1 (MQ=255)

                                                                                       tGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGGGGAGGTGCCTa  <  1:577138/93‑1 (MQ=255)

CAACGCAATTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGTGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGGGGAGGTGCCTA  >  NC_000913/922308‑922487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTGCAACGCAATTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGTGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGGGGAGGTGCCTATGCT  >  NC_000913/922305‑922491

GTGCAACGCAATTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGG                                                                                               >  SRR4302533.3558501/1‑94 (MQ=60)

GTGCAACGCAATTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGC                                                                                               >  SRR4302533.5257251/1‑94 (MQ=60)

GTGCAACGCAATTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGC                                                                                               <  SRR4302533.5156905/94‑1 (MQ=60)

 TGCAACGCAATTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCG                                                                                              >  SRR4302533.6299568/1‑94 (MQ=60)

   CAACGCAATTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAA                                                                                            <  SRR4302533.3345696/94‑1 (MQ=60)

   CAACGCAATTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAA                                                                                            >  SRR4302533.2831102/1‑94 (MQ=60)

    AACGCAATTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAAC                                                                                           >  SRR4302533.6235239/1‑94 (MQ=60)

    AACGCAATTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAAC                                                                                           >  SRR4302533.5732977/1‑94 (MQ=60)

    AACGCAATTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAAC                                                                                           >  SRR4302533.3165091/1‑94 (MQ=60)

       GCAATTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAG                                                                                        <  SRR4302533.2494105/94‑1 (MQ=60)

       GCAATTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAG                                                                                        <  SRR4302533.2275056/94‑1 (MQ=60)

       GCAATTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAG                                                                                        >  SRR4302533.6261706/1‑94 (MQ=60)

        CAATTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGT                                                                                       <  SRR4302533.4200046/94‑1 (MQ=60)

         AATTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTA                                                                                      <  SRR4302533.1877887/94‑1 (MQ=60)

         AATTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTA                                                                                      <  SRR4302533.1186839/94‑1 (MQ=60)

          ATTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTAC                                                                                     <  SRR4302533.6213434/94‑1 (MQ=60)

          ATTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTAC                                                                                     <  SRR4302533.5649561/94‑1 (MQ=60)

          ATTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTAC                                                                                     >  SRR4302533.3739028/1‑94 (MQ=60)

           TTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACG                                                                                    <  SRR4302533.2303315/94‑1 (MQ=60)

           TTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACG                                                                                    >  SRR4302533.5758912/1‑94 (MQ=60)

           TTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACG                                                                                    <  SRR4302533.5207653/94‑1 (MQ=60)

           TTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACG                                                                                    <  SRR4302533.2836171/94‑1 (MQ=60)

            TGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGC                                                                                   >  SRR4302533.6295526/1‑94 (MQ=60)

            TGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGC                                                                                   >  SRR4302533.2435409/1‑94 (MQ=60)

             GATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCG                                                                                  <  SRR4302533.5761440/94‑1 (MQ=60)

             GATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCG                                                                                  >  SRR4302533.5622945/1‑94 (MQ=60)

              ATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGA                                                                                 >  SRR4302533.3586680/1‑94 (MQ=60)

              ATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGA                                                                                 >  SRR4302533.4082032/1‑94 (MQ=60)

               TGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAG                                                                                >  SRR4302533.929329/1‑94 (MQ=60)

               AGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAG                                                                                <  SRR4302533.4969949/94‑1 (MQ=60)

                GCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGG                                                                               <  SRR4302533.4305463/94‑1 (MQ=60)

                 CTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGG                                                                              <  SRR4302533.4779668/94‑1 (MQ=60)

                  TCGCTGTTCACTACCAGGGGAATGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGA                                                                             <  SRR4302533.6062250/94‑1 (MQ=60)

                     CTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAA                                                                          >  SRR4302533.5433787/1‑94 (MQ=60)

                       GTTCACTCCCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATG                                                                        <  SRR4302533.459672/94‑1 (MQ=60)

                          CACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGC                                                                     >  SRR4302533.3162953/1‑94 (MQ=60)

                           ACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCA                                                                    <  SRR4302533.5003135/94‑1 (MQ=60)

                           ACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCA                                                                    <  SRR4302533.5197275/94‑1 (MQ=60)

                           ACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCA                                                                    <  SRR4302533.6062953/94‑1 (MQ=60)

                            CTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCAT                                                                   <  SRR4302533.5820013/94‑1 (MQ=60)

                            CTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCAT                                                                   <  SRR4302533.1639827/94‑1 (MQ=60)

                             TACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATC                                                                  >  SRR4302533.3025285/1‑94 (MQ=60)

                             TACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATC                                                                  <  SRR4302533.878726/94‑1 (MQ=60)

                              ACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCC                                                                 >  SRR4302533.5442694/1‑94 (MQ=60)

                               CCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCA                                                                >  SRR4302533.3301851/1‑94 (MQ=60)

                                CAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCAT                                                               <  SRR4302533.2410049/94‑1 (MQ=60)

                                 AGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATT                                                              >  SRR4302533.2526811/1‑94 (MQ=60)

                                 AGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATT                                                              <  SRR4302533.6066051/94‑1 (MQ=60)

                                 AGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATG                                                              >  SRR4302533.2180251/1‑94 (MQ=60)

                                 AGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGCGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATT                                                              >  SRR4302533.1104274/1‑94 (MQ=60)

                                  GGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTG                                                             <  SRR4302533.3012717/94‑1 (MQ=60)

                                   GGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGC                                                            <  SRR4302533.2460322/94‑1 (MQ=60)

                                    GGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCT                                                           <  SRR4302533.6067955/94‑1 (MQ=60)

                                     GAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTG                                                          <  SRR4302533.5552844/94‑1 (MQ=60)

                                     GAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTG                                                          <  SRR4302533.2557576/94‑1 (MQ=60)

                                      AAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGT                                                         <  SRR4302533.1338285/94‑1 (MQ=60)

                                       AGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTG                                                        >  SRR4302533.304932/1‑94 (MQ=60)

                                        GGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGT                                                       <  SRR4302533.1807809/94‑1 (MQ=60)

                                        GGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGT                                                       <  SRR4302533.1005338/94‑1 (MQ=60)

                                         GCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCTAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTA                                                      >  SRR4302533.2886605/1‑94 (MQ=60)

                                           CATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACG                                                    >  SRR4302533.3943601/1‑94 (MQ=60)

                                           CATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACG                                                    >  SRR4302533.2334601/1‑94 (MQ=60)

                                           CATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACG                                                    >  SRR4302533.4930139/1‑94 (MQ=60)

                                           CATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACG                                                    <  SRR4302533.296015/94‑1 (MQ=60)

                                             TTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCT                                                  >  SRR4302533.1703755/1‑94 (MQ=60)

                                             TTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCT                                                  >  SRR4302533.3320334/1‑94 (MQ=60)

                                               TGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAG                                                <  SRR4302533.3002597/94‑1 (MQ=60)

                                               TGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAG                                                <  SRR4302533.891637/94‑1 (MQ=60)

                                                 CGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAA                                              >  SRR4302533.3266977/1‑94 (MQ=60)

                                                  GGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAA                                             >  SRR4302533.4745315/1‑94 (MQ=60)

                                                  GGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAA                                             >  SRR4302533.2121581/1‑94 (MQ=60)

                                                  GGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAA                                             >  SRR4302533.4714847/1‑94 (MQ=60)

                                                   GAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAA                                            >  SRR4302533.5549608/1‑94 (MQ=60)

                                                      TCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGC                                         >  SRR4302533.5781272/1‑94 (MQ=60)

                                                      TCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGC                                         >  SRR4302533.1557738/1‑94 (MQ=60)

                                                       CTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCC                                        >  SRR4302533.5991581/1‑94 (MQ=60)

                                                        TTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCT                                       <  SRR4302533.4820062/94‑1 (MQ=60)

                                                          TACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGA                                     <  SRR4302533.595811/94‑1 (MQ=60)

                                                           ACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAA                                    >  SRR4302533.4440519/1‑94 (MQ=60)

                                                            CCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAAT                                   <  SRR4302533.511947/94‑1 (MQ=60)

                                                            CCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAAT                                   <  SRR4302533.4632657/94‑1 (MQ=60)

                                                             CGCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATG                                  <  SRR4302533.4701345/94‑1 (MQ=60)

                                                              GCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGC                                 >  SRR4302533.5026306/1‑94 (MQ=60)

                                                              GCCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGC                                 <  SRR4302533.2564350/94‑1 (MQ=60)

                                                               CCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCA                                >  SRR4302533.4181192/1‑94 (MQ=60)

                                                               CCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCA                                <  SRR4302533.5719024/94‑1 (MQ=60)

                                                               CCGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCA                                >  SRR4302533.2283359/1‑94 (MQ=60)

                                                                CGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGACTGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAG                               <  SRR4302533.4458076/94‑1 (MQ=60)

                                                                CGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAG                               <  SRR4302533.2242647/94‑1 (MQ=60)

                                                                CGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAG                               <  SRR4302533.3748953/94‑1 (MQ=60)

                                                                CGAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAG                               >  SRR4302533.3358076/1‑94 (MQ=60)

                                                                 GAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGG                              <  SRR4302533.2561228/94‑1 (MQ=60)

                                                                 GAGGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGG                              >  SRR4302533.4377520/1‑94 (MQ=60)

                                                                   GGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCA                            >  SRR4302533.3835284/1‑94 (MQ=60)

                                                                   GGTTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCA                            >  SRR4302533.866325/1‑94 (MQ=60)

                                                                     TTGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATA                          <  SRR4302533.2284841/94‑1 (MQ=60)

                                                                      TGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAA                         <  SRR4302533.949392/94‑1 (MQ=60)

                                                                      TGCAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAA                         <  SRR4302533.5888045/94‑1 (MQ=60)

                                                                        CAGAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAA                       >  SRR4302533.2258063/1‑94 (MQ=60)

                                                                          GAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAAT                     <  SRR4302533.5024212/94‑1 (MQ=60)

                                                                          GAAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAAT                     >  SRR4302533.4032976/1‑94 (MQ=60)

                                                                           AAACCAAAGTGGCGATGGCGAACAAGTACGCGATGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATT                    <  SRR4302533.2247407/94‑1 (MQ=60)

                                                                           AAACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATT                    <  SRR4302533.5061179/94‑1 (MQ=60)

                                                                             ACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGG                  <  SRR4302533.1109119/94‑1 (MQ=60)

                                                                             ACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGG                  <  SRR4302533.6109282/94‑1 (MQ=60)

                                                                             ACCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGG                  <  SRR4302533.5738206/94‑1 (MQ=60)

                                                                              CCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGG                 <  SRR4302533.392074/94‑1 (MQ=60)

                                                                              CCAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGG                 >  SRR4302533.4896601/1‑94 (MQ=60)

                                                                               CAAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGGG                >  SRR4302533.6183283/1‑94 (MQ=60)

                                                                                AAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGGGG               <  SRR4302533.4476592/94‑1 (MQ=60)

                                                                                AAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGGGG               <  SRR4302533.4995021/94‑1 (MQ=60)

                                                                                AAAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGGGG               >  SRR4302533.662061/1‑94 (MQ=60)

                                                                                 AAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGGGGA              >  SRR4302533.6160970/1‑94 (MQ=60)

                                                                                 AAGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGGGGA              <  SRR4302533.758217/94‑1 (MQ=60)

                                                                                  AGTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGGGGAG             <  SRR4302533.593454/94‑1 (MQ=60)

                                                                                   GTGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGGGGAGG            >  SRR4302533.4708795/1‑94 (MQ=60)

                                                                                    TGGCGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGGGGAGGG           >  SRR4302533.5770649/1‑94 (MQ=60)

                                                                                       CGATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGGGGAGGTGCC        >  SRR4302533.529240/1‑94 (MQ=60)

                                                                                        GATGTCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACACTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGGGGAGGTGCCT       <  SRR4302533.4541175/94‑1 (MQ=60)

                                                                                         ATGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGGGGAGGTGCCTA      <  SRR4302533.6061974/94‑1 (MQ=60)

                                                                                          TGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGGGGAGGTGCCTAT     >  SRR4302533.4885918/1‑94 (MQ=60)

                                                                                          TGGCGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGGGGAGGTGCCTAT     <  SRR4302533.580542/94‑1 (MQ=60)

                                                                                             CGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGGGGAGGTGCCTATGCT  >  SRR4302533.6142328/1‑94 (MQ=60)

                                                                                             CGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAACCCTGAATGCAGGCATAAAAATTGGGGGAGGTGCCTATGCT  <  SRR4302533.5867241/94‑1 (MQ=60)

GTGCAACGCAATTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGTGAACAAGTACGCGAGGGAGAATGAGCATCCATTGCTGTGTACGCTAGAAAAAGCCTGAATGCAGGCATAAAAATTGGGGGAGGTGCCTATGCT  >  NC_000913/922305‑922491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`