Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,005,090 C→T intergenic (+89/‑85) pyrD → / → ycbW dihydro‑orotate dehydrogenase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,005,0900CT100.0% 708.9 / NA 188intergenic (+89/‑85)pyrD/ycbWdihydro‑orotate dehydrogenase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (107/81);  total (107/81)

Only 100 of 190 total aligned reads displayed.

AATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCAGATGC  >  NC_000913/1005000‑1005178
                                                                                          |                                                                                        
aaTTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGatat                                                                                        >  1:1037946/1‑93 (MQ=255)
aaTTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGatat                                                                                        >  1:4721806/1‑93 (MQ=255)
 attattTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATAtt                                                                                       >  2:2762786/1‑93 (MQ=255)
 attattTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATAtt                                                                                       <  1:6232335/93‑1 (MQ=255)
 attattTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATAtt                                                                                       <  1:5217717/93‑1 (MQ=255)
  ttattTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATAttt                                                                                      >  2:3215926/1‑93 (MQ=255)
   tattTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATAtttt                                                                                     <  2:286889/93‑1 (MQ=255)
    attTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTg                                                                                    <  1:1738316/93‑1 (MQ=255)
    attTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTg                                                                                    >  2:3843341/1‑93 (MQ=255)
         ttcGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACtt                                                                               <  2:5738115/93‑1 (MQ=255)
         ttcGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTAGTTAGATATTTTGTACtt                                                                               <  2:411834/93‑1 (MQ=255)
            gAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTa                                                                            >  2:1250193/1‑93 (MQ=255)
            gAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTa                                                                            <  2:2624429/93‑1 (MQ=255)
             aaTTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTAt                                                                           >  1:4528906/1‑93 (MQ=255)
             aaTTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTAt                                                                           >  1:1934718/1‑93 (MQ=255)
                  ctctTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTAttgttg                                                                      >  1:364216/1‑93 (MQ=255)
                     tttATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAgg                                                                   >  1:738703/1‑93 (MQ=255)
                     tttATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAgg                                                                   >  2:3112458/1‑93 (MQ=255)
                     tttATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAgg                                                                   >  2:3714090/1‑93 (MQ=255)
                       tataAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGtt                                                                 >  1:1475236/1‑93 (MQ=255)
                       tataAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGtt                                                                 >  2:174473/1‑93 (MQ=255)
                        ataAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGtta                                                                >  2:1532198/1‑93 (MQ=255)
                           aGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGttatta                                                             <  2:556127/93‑1 (MQ=255)
                              tAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTaagc                                                          >  1:28801/1‑93 (MQ=255)
                              tAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTaagc                                                          <  1:3432971/93‑1 (MQ=255)
                               aaTCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTaagcg                                                         >  1:4858881/1‑93 (MQ=255)
                                aTCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTaagcga                                                        <  1:3800287/93‑1 (MQ=255)
                                aTCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTaagcga                                                        >  2:2296796/1‑93 (MQ=255)
                                 tCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTaagcgaa                                                       >  2:4156201/1‑93 (MQ=255)
                                  cAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTaagcgaag                                                      <  1:2720442/93‑1 (MQ=255)
                                   aGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTaagcgaagc                                                     >  1:2253080/1‑93 (MQ=255)
                                    gggCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTaagcgaagcg                                                    >  1:561112/1‑93 (MQ=255)
                                     ggCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAagcgaagcga                                                   >  2:5820938/1‑93 (MQ=255)
                                       cTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACa                                                 >  1:4761163/1‑93 (MQ=255)
                                        tttATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACaa                                                <  2:1280629/93‑1 (MQ=255)
                                          tATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATg                                              <  2:5987796/93‑1 (MQ=255)
                                          tATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATg                                              <  1:3076794/93‑1 (MQ=255)
                                          tATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATg                                              >  1:2045467/1‑93 (MQ=255)
                                          tATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATg                                              <  1:752707/93‑1 (MQ=255)
                                           aTTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATgg                                             <  2:3005901/93‑1 (MQ=255)
                                           aTTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATgg                                             >  1:2179441/1‑93 (MQ=255)
                                            ttttCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGa                                            >  1:4583689/1‑93 (MQ=255)
                                             tttCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGAt                                           <  2:2722868/93‑1 (MQ=255)
                                              ttCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGAtt                                          <  1:5266767/93‑1 (MQ=255)
                                               tCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATtg                                         <  1:3860715/93‑1 (MQ=255)
                                                cAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGTTATTTTGTAGTTTTTTTGTTGAGGTTATTAAGCGAAGCGCCAACAGATtgt                                        >  2:1103188/1‑93 (MQ=255)
                                                cAGCCCTGGTTGTCTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATtgt                                        <  1:3603373/93‑1 (MQ=255)
                                                  gCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTgtgt                                      <  2:241023/93‑1 (MQ=255)
                                                   cccTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGtt                                     <  1:5770472/93‑1 (MQ=255)
                                                    ccTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTtg                                    <  2:4447294/93‑1 (MQ=255)
                                                     cTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTtgc                                   <  1:1857350/93‑1 (MQ=255)
                                                     cTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTtgc                                   >  2:3149236/1‑93 (MQ=255)
                                                     cTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTtgc                                   >  2:3879571/1‑93 (MQ=255)
                                                     cTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTtgc                                   >  1:2288393/1‑93 (MQ=255)
                                                      tGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTtgct                                  <  1:5512338/93‑1 (MQ=255)
                                                       ggTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTtgctg                                 >  2:3808170/1‑93 (MQ=255)
                                                       ggTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTtgctg                                 >  2:267946/1‑93 (MQ=255)
                                                        gttgttTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTtggtgc                                >  1:1626000/1‑93 (MQ=255)
                                                         ttgttTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCg                               >  2:3313979/1‑93 (MQ=255)
                                                          tgttTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCgg                              >  2:664071/1‑93 (MQ=255)
                                                          tgttTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCgg                              >  2:1882871/1‑93 (MQ=255)
                                                           gttTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGt                             >  1:4723954/1‑93 (MQ=255)
                                                             tttATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGttt                           <  2:6256152/93‑1 (MQ=255)
                                                                 tatTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTtata                       >  2:6087511/1‑93 (MQ=255)
                                                                 tatTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTtata                       >  2:781987/1‑93 (MQ=255)
                                                                 tatTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTtata                       >  1:2341054/1‑93 (MQ=255)
                                                                  atTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTTtag                      >  1:5334209/1‑93 (MQ=255)
                                                                  atTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTAtag                      <  1:4312662/93‑1 (MQ=255)
                                                                    tCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTAtaggg                    <  2:2777873/93‑1 (MQ=255)
                                                                    tCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTAtaggg                    <  2:342661/93‑1 (MQ=255)
                                                                    tCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTAtaggg                    <  2:606555/93‑1 (MQ=255)
                                                                     cATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTAtagggt                   >  1:1350502/1‑93 (MQ=255)
                                                                     cATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTAtagggt                   >  1:4429112/1‑93 (MQ=255)
                                                                     cATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTAtagggt                   <  2:5338891/93‑1 (MQ=255)
                                                                      aTCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTAtagggta                  <  2:1593496/93‑1 (MQ=255)
                                                                       tCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTAtagggtag                 >  1:5873484/1‑93 (MQ=255)
                                                                       tCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTAtagggtag                 <  1:4751274/93‑1 (MQ=255)
                                                                       tCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTAtagggtag                 >  1:2425768/1‑93 (MQ=255)
                                                                         gCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTAtagggtaggg               >  1:888054/1‑93 (MQ=255)
                                                                          cTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGAAgggg              >  2:4747344/1‑93 (MQ=255)
                                                                             ttGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGgggaggggagg           >  2:3374179/1‑92 (MQ=255)
                                                                              tGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGagag          >  2:4800977/1‑93 (MQ=255)
                                                                               gCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAgg         >  2:686583/1‑93 (MQ=255)
                                                                                cTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGc        <  1:5176886/93‑1 (MQ=255)
                                                                                cTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGc        <  2:4627289/93‑1 (MQ=255)
                                                                                 ttATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCa       <  2:5258473/93‑1 (MQ=255)
                                                                                  tATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCAg      >  1:1069216/1‑93 (MQ=255)
                                                                                   aTTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCAGa     <  2:1074569/93‑1 (MQ=255)
                                                                                   aTTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCAGa     <  2:1357408/93‑1 (MQ=255)
                                                                                   aTTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCAGa     <  2:2032437/93‑1 (MQ=255)
                                                                                    tttAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCAGAt    <  2:392968/93‑1 (MQ=255)
                                                                                    tttAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCAGAt    <  1:3214660/93‑1 (MQ=255)
                                                                                    tttAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCAGAt    >  1:5976752/1‑93 (MQ=255)
                                                                                     ttAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCAGATg   <  2:3556592/93‑1 (MQ=255)
                                                                                     ttAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGGAGGGGAGAGGCAGATg   >  2:2660263/1‑93 (MQ=255)
                                                                                      tAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCAGATGc  <  2:4565034/93‑1 (MQ=255)
                                                                                      tAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCAGATGc  <  1:4130699/93‑1 (MQ=255)
                                                                                      tAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCAGATGc  <  1:3289369/93‑1 (MQ=255)
                                                                                      tAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCAGATGc  >  1:2619073/1‑93 (MQ=255)
                                                                                      tAGATATTTTGTACTTTTATTGTTGAGGGTATTAAGCGAAGCGACAATGGATTGTGCTGCTGCGGTTTTCTAGGGTAGGGGAGAGGCAGCTGc  >  2:6226601/1‑93 (MQ=255)
                                                                                          |                                                                                        
AATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCAGATGC  >  NC_000913/1005000‑1005178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCAGATGCGA  >  NC_000913/1004997‑1005180
                                                                                             |                                                                                          
TCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGAT                                                                                            <  SRR4302533.5097041/94‑1 (MQ=60)
TCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGAT                                                                                            <  SRR4302533.2361156/94‑1 (MQ=60)
 CTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATA                                                                                           <  SRR4302533.5620388/94‑1 (MQ=60)
 CTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATA                                                                                           >  SRR4302533.3799587/1‑94 (MQ=60)
 CTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATA                                                                                           <  SRR4302533.3743680/94‑1 (MQ=60)
 CTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATA                                                                                           >  SRR4302533.2599121/1‑94 (MQ=60)
 CCAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATA                                                                                           >  SRR4302533.5979144/1‑94 (MQ=60)
  TAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATAT                                                                                          >  SRR4302533.1044046/1‑94 (MQ=60)
  TAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATAT                                                                                          <  SRR4302533.4974667/94‑1 (MQ=60)
  TAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATAT                                                                                          >  SRR4302533.4750436/1‑94 (MQ=60)
  TAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATAT                                                                                          <  SRR4302533.156846/94‑1 (MQ=60)
    ATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTT                                                                                        <  SRR4302533.6269658/94‑1 (MQ=60)
    ATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTT                                                                                        <  SRR4302533.5249127/94‑1 (MQ=60)
      TATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTG                                                                                      >  SRR4302533.3037925/1‑94 (MQ=60)
       ATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGT                                                                                     <  SRR4302533.1748861/94‑1 (MQ=60)
        TTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTA                                                                                    >  SRR4302533.1903330/1‑94 (MQ=60)
         TTCTTCGAATTCCTCTTTATAAGATAATCAGTGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTAC                                                                                   <  SRR4302533.3680850/94‑1 (MQ=60)
           CTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTT                                                                                 >  SRR4302533.755848/1‑94 (MQ=60)
           CTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTT                                                                                 >  SRR4302533.4912072/1‑94 (MQ=60)
               GAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTAT                                                                             >  SRR4302533.1946521/1‑94 (MQ=60)
               GAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTAT                                                                             >  SRR4302533.4556333/1‑94 (MQ=60)
                AATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATT                                                                            >  SRR4302533.4302602/1‑94 (MQ=60)
                   TCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTT                                                                         <  SRR4302533.4596741/94‑1 (MQ=60)
                   TCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTT                                                                         <  SRR4302533.2341452/94‑1 (MQ=60)
                    CCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTG                                                                        >  SRR4302533.366425/1‑94 (MQ=60)
                       CTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGG                                                                     >  SRR4302533.743053/1‑94 (MQ=60)
                         TTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTT                                                                   >  SRR4302533.2588953/1‑94 (MQ=60)
                         TTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTT                                                                   <  SRR4302533.5301695/94‑1 (MQ=60)
                         TTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTT                                                                   >  SRR4302533.1484153/1‑94 (MQ=60)
                            TAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATT                                                                >  SRR4302533.4493854/1‑94 (MQ=60)
                                ATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGC                                                            >  SRR4302533.701996/1‑94 (MQ=60)
                                ATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGC                                                            >  SRR4302533.28996/1‑94 (MQ=60)
                                 TAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCG                                                           >  SRR4302533.4888332/1‑94 (MQ=60)
                                 TAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCG                                                           <  SRR4302533.3454180/94‑1 (MQ=60)
                                  AATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGA                                                          >  SRR4302533.6164338/1‑94 (MQ=60)
                                   ATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAA                                                         <  SRR4302533.3823547/94‑1 (MQ=60)
                                     CAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGC                                                       <  SRR4302533.2737138/94‑1 (MQ=60)
                                     CAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGC                                                       >  SRR4302533.2267014/1‑94 (MQ=60)
                                      AGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCG                                                      >  SRR4302533.564440/1‑94 (MQ=60)
                                      AGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCG                                                      >  SRR4302533.1526815/1‑94 (MQ=60)
                                         GCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACA                                                   >  SRR4302533.4790046/1‑94 (MQ=60)
                                         GCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACA                                                   <  SRR4302533.6002423/94‑1 (MQ=60)
                                         GCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCATATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACA                                                   >  SRR4302533.3409298/1‑94 (MQ=60)
                                           TTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAAT                                                 >  SRR4302533.2717332/1‑94 (MQ=60)
                                           TTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAAT                                                 >  SRR4302533.5261345/1‑94 (MQ=60)
                                            TTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATG                                                >  SRR4302533.2058077/1‑94 (MQ=60)
                                             TATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGG                                               <  SRR4302533.3095677/94‑1 (MQ=60)
                                             TATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGG                                               <  SRR4302533.757144/94‑1 (MQ=60)
                                             TATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGG                                               >  SRR4302533.2192878/1‑94 (MQ=60)
                                              ATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGA                                              >  SRR4302533.4611458/1‑94 (MQ=60)
                                                 TTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTG                                           <  SRR4302533.5298368/94‑1 (MQ=60)
                                                  TCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGT                                          <  SRR4302533.3884282/94‑1 (MQ=60)
                                                   CAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTG                                         >  SRR4302533.3860469/1‑94 (MQ=60)
                                                   CAGCCCTGGTTGTCTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTG                                         <  SRR4302533.3625551/94‑1 (MQ=60)
                                                     GCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTT                                       <  SRR4302533.2155071/94‑1 (MQ=60)
                                                     GCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTT                                       <  SRR4302533.3235753/94‑1 (MQ=60)
                                                      CCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTG                                      <  SRR4302533.5805313/94‑1 (MQ=60)
                                                       CCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGC                                     >  SRR4302533.2302562/1‑94 (MQ=60)
                                                       CCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGC                                     >  SRR4302533.1726264/1‑94 (MQ=60)
                                                        CTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCT                                    <  SRR4302533.1868677/94‑1 (MQ=60)
                                                        CTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCT                                    >  SRR4302533.5386507/1‑94 (MQ=60)
                                                         TGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTG                                   <  SRR4302533.5545615/94‑1 (MQ=60)
                                                          GGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGGTGC                                  >  SRR4302533.1635849/1‑94 (MQ=60)
                                                          GGTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGC                                  >  SRR4302533.1327691/1‑94 (MQ=60)
                                                           GTTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCG                                 >  SRR4302533.88981/1‑94 (MQ=60)
                                                            TTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACTATGGATTGTGTTGCTGCGG                                >  SRR4302533.141849/1‑94 (MQ=60)
                                                            TTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGG                                <  SRR4302533.1951009/94‑1 (MQ=60)
                                                            TTGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGG                                <  SRR4302533.588615/94‑1 (MQ=60)
                                                             TGTTTTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGT                               >  SRR4302533.4752597/1‑94 (MQ=60)
                                                                 TTATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGTATTGTGTTGCTGCGGTTTTA                           <  SRR4302533.1830148/94‑1 (MQ=60)
                                                                   ATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATA                         >  SRR4302533.2355547/1‑94 (MQ=60)
                                                                   ATATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATA                         <  SRR4302533.4007604/94‑1 (MQ=60)
                                                                    TATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTTTAG                        >  SRR4302533.5366183/1‑94 (MQ=60)
                                                                    TATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAG                        >  SRR4302533.5612387/1‑94 (MQ=60)
                                                                     ATTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGG                       <  SRR4302533.4338625/94‑1 (MQ=60)
                                                                      TTCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGG                      >  SRR4302533.441732/1‑94 (MQ=60)
                                                                       TCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGT                     >  SRR4302533.4455887/1‑94 (MQ=60)
                                                                       TCATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGT                     >  SRR4302533.1358623/1‑94 (MQ=60)
                                                                        CATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTA                    >  SRR4302533.2366830/1‑94 (MQ=60)
                                                                        CATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTA                    >  SRR4302533.4755418/1‑94 (MQ=60)
                                                                        CATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTA                    >  SRR4302533.2232373/1‑94 (MQ=60)
                                                                         ATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAG                   >  SRR4302533.5245110/1‑94 (MQ=60)
                                                                         ATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAG                   >  SRR4302533.2440745/1‑94 (MQ=60)
                                                                         ATCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAG                   >  SRR4302533.5908985/1‑94 (MQ=60)
                                                                          TCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGG                  <  SRR4302533.4780080/94‑1 (MQ=60)
                                                                          TCGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGG                  <  SRR4302533.4780081/94‑1 (MQ=60)
                                                                           CGCTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGG                 >  SRR4302533.893267/1‑94 (MQ=60)
                                                                             CTGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGA               <  SRR4302533.4830285/94‑1 (MQ=60)
                                                                              TGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAG              >  SRR4302533.4731030/1‑94 (MQ=60)
                                                                              TGTTGCTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAG              >  SRR4302533.2802294/1‑94 (MQ=60)
                                                                                   CTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCA         <  SRR4302533.5208153/94‑1 (MQ=60)
                                                                                   CTTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCA         >  SRR4302533.3170526/1‑94 (MQ=60)
                                                                                    TTATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCAG        >  SRR4302533.1075511/1‑94 (MQ=60)
                                                                                      ATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCAGAT      >  SRR4302533.6012951/1‑94 (MQ=60)
                                                                                      ATTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCAGAT      <  SRR4302533.3651604/94‑1 (MQ=60)
                                                                                       TTTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCAGATG     <  SRR4302533.3234479/94‑1 (MQ=60)
                                                                                        TTAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCAGATGC    >  SRR4302533.2635151/1‑94 (MQ=60)
                                                                                         TAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCAGATGCG   <  SRR4302533.4155772/94‑1 (MQ=60)
                                                                                         TAGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCAGATGCG   <  SRR4302533.3309677/94‑1 (MQ=60)
                                                                                          AGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCAGATGCGA  >  SRR4302533.5359211/1‑94 (MQ=60)
                                                                                          AGATATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCAGATGCGA  >  SRR4302533.4214281/1‑94 (MQ=60)
                                                                                             |                                                                                          
TCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCAGATGCGA  >  NC_000913/1004997‑1005180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑