Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,009,201 T→C S5S (AGT→AGC)  uup → putative ATP‑binding component of a transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,009,2010TC99.6% 731.4 / NA 225S5S (AGT→AGC) uupputative ATP‑binding component of a transport system
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (126/98);  minor base G (1/0);  total (127/98)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 233 total aligned reads displayed.

CGCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGTATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTGTCTGGT  >  NC_000913/1009111‑1009290
                                                                                          |                                                                                         
cGCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCAt                                                                                         <  2:5730109/93‑1 (MQ=255)
  ctctCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATgc                                                                                       >  1:4176289/1‑93 (MQ=255)
   tctcCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATgca                                                                                      >  1:4815471/1‑93 (MQ=255)
   tctcCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATgca                                                                                      >  2:5095177/1‑93 (MQ=255)
   tctcCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATgca                                                                                      <  2:2017047/93‑1 (MQ=255)
   tctcCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATgca                                                                                      >  2:5391137/1‑93 (MQ=255)
    ctcCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAgcatgcat                                                                                     <  1:6010885/93‑1 (MQ=255)
     tcCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGcatgcatg                                                                                    >  1:2311006/1‑93 (MQ=255)
      cccAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATgcatgg                                                                                   >  1:753949/1‑93 (MQ=255)
       ccAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATgcatggc                                                                                  <  2:1442539/93‑1 (MQ=255)
        cAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATgcatggcg                                                                                 >  2:5222582/1‑93 (MQ=255)
         aGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATgcatggcgc                                                                                <  1:1703655/93‑1 (MQ=255)
         aGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATgcatggcgc                                                                                >  2:4494237/1‑93 (MQ=255)
          ggATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATgcatggcgca                                                                               >  2:1037939/1‑93 (MQ=255)
          ggATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATgcatggcgca                                                                               <  2:2953550/93‑1 (MQ=255)
           gATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATgcatggcgcat                                                                              <  2:3380243/93‑1 (MQ=255)
           gATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATgcatggcgcat                                                                              >  1:859476/1‑93 (MQ=255)
           gATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATCACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATgcatggcgcat                                                                              >  1:276043/1‑93 (MQ=255)
            aTTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATgcatggcgcatg                                                                             <  2:3393472/93‑1 (MQ=255)
             tttCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATgcatggcgcatgg                                                                            >  1:6187635/1‑93 (MQ=255)
              ttCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATgcatggcgcatggc                                                                           <  1:4700945/93‑1 (MQ=255)
              ttCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATgcatggcgcatggc                                                                           <  2:2926212/93‑1 (MQ=255)
                 gCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGt                                                                        <  1:3905331/93‑1 (MQ=255)
                 gCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGt                                                                        >  1:5854806/1‑93 (MQ=255)
                  cccGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTc                                                                       <  2:843650/93‑1 (MQ=255)
                  cccGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTc                                                                       >  1:4130879/1‑93 (MQ=255)
                  cccGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTc                                                                       >  2:4407078/1‑93 (MQ=255)
                      tAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTc                                                                   >  1:196119/1‑93 (MQ=255)
                      tAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTc                                                                   >  1:2491948/1‑93 (MQ=255)
                       aaCCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCa                                                                  >  1:841148/1‑93 (MQ=255)
                       aaCCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCa                                                                  <  2:613574/93‑1 (MQ=255)
                        aCCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAg                                                                 <  2:3979110/93‑1 (MQ=255)
                         ccGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGc                                                                <  1:5205241/93‑1 (MQ=255)
                           gCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGa                                                              >  1:347686/1‑93 (MQ=255)
                           gCCAGATCCACAACTGCTGGCCGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGa                                                              >  2:4011991/1‑93 (MQ=255)
                            ccAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGAc                                                             <  1:5796281/93‑1 (MQ=255)
                            ccAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGAc                                                             <  1:624752/93‑1 (MQ=255)
                            ccAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGAc                                                             >  1:1877545/1‑93 (MQ=255)
                             cAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGAcg                                                            >  2:5779691/1‑93 (MQ=255)
                              aGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGAcgc                                                           >  2:2049525/1‑93 (MQ=255)
                               gATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGAcgcg                                                          >  2:1184842/1‑93 (MQ=255)
                               gATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGAcgcg                                                          <  2:5979635/93‑1 (MQ=255)
                               gATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGAcgcg                                                          >  2:376278/1‑93 (MQ=255)
                               gATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGAcgcg                                                          >  1:1303144/1‑93 (MQ=255)
                                aTCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACgcgc                                                         <  2:5790467/93‑1 (MQ=255)
                                 tCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGcgcc                                                        >  2:3306540/1‑93 (MQ=255)
                                 tCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGcgcc                                                        <  1:1500599/93‑1 (MQ=255)
                                   cacaACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGcgccgc                                                      <  2:2833476/93‑1 (MQ=255)
                                    acaACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCt                                                     >  1:2386941/1‑93 (MQ=255)
                                    acaACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCt                                                     >  2:1920359/1‑93 (MQ=255)
                                     caACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCtt                                                    >  1:4927016/1‑93 (MQ=255)
                                      aaCTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTtc                                                   >  1:3132493/1‑93 (MQ=255)
                                      aaCTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTtc                                                   >  1:5529094/1‑93 (MQ=255)
                                          gctggctgATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGa                                               <  1:6023626/93‑1 (MQ=255)
                                          gctggctgATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGa                                               <  1:1033300/93‑1 (MQ=255)
                                           ctggctgATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGAt                                              <  1:1069353/93‑1 (MQ=255)
                                             ggctgATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATaa                                            <  2:1735000/93‑1 (MQ=255)
                                             ggctgATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATaa                                            >  2:1302376/1‑93 (MQ=255)
                                             ggctgATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATaa                                            >  2:6022382/1‑93 (MQ=255)
                                              gctgATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAAc                                           >  1:209788/1‑93 (MQ=255)
                                              gctgATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAAc                                           <  2:34614/93‑1 (MQ=255)
                                              gctgATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCAGGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAAc                                           <  1:4437910/93‑1 (MQ=255)
                                               ctgATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACg                                          <  2:1825895/93‑1 (MQ=255)
                                                tgATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGc                                         >  2:4241216/1‑93 (MQ=255)
                                                   ttACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGa                                      >  2:5899020/1‑93 (MQ=255)
                                                   ttACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGa                                      <  2:4634647/93‑1 (MQ=255)
                                                    tACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGaa                                     <  1:3439028/93‑1 (MQ=255)
                                                      ccGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACt                                   <  2:474814/93‑1 (MQ=255)
                                                        gCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGc                                 >  2:3662745/1‑93 (MQ=255)
                                                        gCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGc                                 >  1:5614541/1‑93 (MQ=255)
                                                        gCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGc                                 >  2:4072033/1‑93 (MQ=255)
                                                        gCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGc                                 >  1:2627546/1‑93 (MQ=255)
                                                        gCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGc                                 >  1:5584400/1‑93 (MQ=255)
                                                            ccTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCatat                             <  2:2480844/93‑1 (MQ=255)
                                                             cTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATc                            <  1:3786992/93‑1 (MQ=255)
                                                             cTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATc                            <  1:559410/93‑1 (MQ=255)
                                                              tGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCg                           <  1:5725189/93‑1 (MQ=255)
                                                              tGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCg                           >  2:3034852/1‑93 (MQ=255)
                                                              tGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCg                           >  2:1645405/1‑93 (MQ=255)
                                                              tGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCg                           >  2:4861196/1‑93 (MQ=255)
                                                               gAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGa                          >  2:2615722/1‑93 (MQ=255)
                                                                 aaGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAg                        <  2:5535020/93‑1 (MQ=255)
                                                                  aGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGa                       <  2:709821/93‑1 (MQ=255)
                                                                  aGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGa                       <  2:1600051/93‑1 (MQ=255)
                                                                      aTAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATaac                   >  1:2125636/1‑93 (MQ=255)
                                                                       tAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATaacg                  >  2:730569/1‑93 (MQ=255)
                                                                        aGTAATGTCATTAATCAGCATGCATGGCGCATGGGTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATaacga                 >  2:4211885/1‑93 (MQ=255)
                                                                          tAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATaacgaac               >  2:2545077/1‑93 (MQ=255)
                                                                          tAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATaacgaac               <  1:2954210/93‑1 (MQ=255)
                                                                          tAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATaacgaac               <  1:17986/93‑1 (MQ=255)
                                                                           aaTGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATaacgaacg              <  2:3595620/93‑1 (MQ=255)
                                                                               tCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGtt          <  1:951000/93‑1 (MQ=255)
                                                                               tCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGtt          >  1:4547307/1‑93 (MQ=255)
                                                                                cATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGttt         >  2:3342598/1‑93 (MQ=255)
                                                                                  ttAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTGt       >  1:4841169/1‑93 (MQ=255)
                                                                                  ttAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTGt       >  1:2965660/1‑93 (MQ=255)
                                                                                    aaTCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTGTCt     <  1:5185504/93‑1 (MQ=255)
                                                                                     aTCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTGTCtg    >  1:5994802/1‑93 (MQ=255)
                                                                                      tCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTGTCtgg   >  2:1990734/1‑93 (MQ=255)
                                                                                      tCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTGTCtg    >  1:224050/1‑92 (MQ=255)
                                                                                          |                                                                                         
CGCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGTATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTGTCTGGT  >  NC_000913/1009111‑1009290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AACGCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGTATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTGTCTGGTGGGC  >  NC_000913/1009109‑1009294
                                                                                            |                                                                                             
AACGCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCA                                                                                              <  SRR4302533.3457783/94‑1 (MQ=60)
 ACGCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCAT                                                                                             <  SRR4302533.5230747/94‑1 (MQ=60)
 ACGCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCAT                                                                                             <  SRR4302533.5755247/94‑1 (MQ=60)
 ACGCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCAT                                                                                             <  SRR4302533.3340162/94‑1 (MQ=60)
 ACGCTCTCCCAGGATTTCGCCAGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCAT                                                                                             <  SRR4302533.2396921/94‑1 (MQ=60)
  CGCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATG                                                                                            <  SRR4302533.2288774/94‑1 (MQ=60)
   GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGC                                                                                           >  SRR4302533.4201593/1‑94 (MQ=60)
   GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGC                                                                                           >  SRR4302533.1741045/1‑94 (MQ=60)
    CTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCA                                                                                          >  SRR4302533.4844657/1‑94 (MQ=60)
      CTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATG                                                                                        <  SRR4302533.6047301/94‑1 (MQ=60)
      CTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATG                                                                                        <  SRR4302533.556382/94‑1 (MQ=60)
      CTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATG                                                                                        >  SRR4302533.2325324/1‑94 (MQ=60)
      CTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATG                                                                                        <  SRR4302533.5980646/94‑1 (MQ=60)
      CTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATG                                                                                        >  SRR4302533.2462634/1‑94 (MQ=60)
       TCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGG                                                                                       <  SRR4302533.4832317/94‑1 (MQ=60)
       TCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGG                                                                                       >  SRR4302533.758388/1‑94 (MQ=60)
        CCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGC                                                                                      <  SRR4302533.2463633/94‑1 (MQ=60)
        CCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGC                                                                                      >  SRR4302533.1831150/1‑94 (MQ=60)
         CCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCG                                                                                     >  SRR4302533.2520740/1‑94 (MQ=60)
           AGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCA                                                                                   >  SRR4302533.4158259/1‑94 (MQ=60)
           AGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCA                                                                                   <  SRR4302533.1713975/94‑1 (MQ=60)
           AGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCA                                                                                   >  SRR4302533.5691201/1‑94 (MQ=60)
            GGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCAT                                                                                  >  SRR4302533.864513/1‑94 (MQ=60)
            GGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATCACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCAT                                                                                  >  SRR4302533.277724/1‑94 (MQ=60)
             GATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATG                                                                                 <  SRR4302533.1957254/94‑1 (MQ=60)
              ATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGG                                                                                >  SRR4302533.6224774/1‑94 (MQ=60)
                TTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCT                                                                              <  SRR4302533.4729449/94‑1 (MQ=60)
                TTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCT                                                                              <  SRR4302533.5090676/94‑1 (MQ=60)
                TTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCT                                                                              <  SRR4302533.3126029/94‑1 (MQ=60)
                  CGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGT                                                                            >  SRR4302533.5890203/1‑94 (MQ=60)
                   GCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTC                                                                           >  SRR4302533.4155952/1‑94 (MQ=60)
                   GCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTC                                                                           <  SRR4302533.3929182/94‑1 (MQ=60)
                      CGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTT                                                                        >  SRR4302533.747406/1‑94 (MQ=60)
                       GTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTC                                                                       >  SRR4302533.2507296/1‑94 (MQ=60)
                       GTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTC                                                                       >  SRR4302533.197357/1‑94 (MQ=60)
                        TAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCA                                                                      <  SRR4302533.2247569/94‑1 (MQ=60)
                        TAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCA                                                                      >  SRR4302533.846066/1‑94 (MQ=60)
                          ACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGC                                                                    >  SRR4302533.936241/1‑94 (MQ=60)
                           CCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCG                                                                   <  SRR4302533.5236607/94‑1 (MQ=60)
                            CGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGA                                                                  >  SRR4302533.349806/1‑94 (MQ=60)
                             GCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGAC                                                                 >  SRR4302533.202650/1‑94 (MQ=60)
                             GCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGAC                                                                 >  SRR4302533.1888988/1‑94 (MQ=60)
                             GCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGAC                                                                 <  SRR4302533.321731/94‑1 (MQ=60)
                             GCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGAC                                                                 <  SRR4302533.6247782/94‑1 (MQ=60)
                              CCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACG                                                                >  SRR4302533.5568435/1‑94 (MQ=60)
                              CCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACG                                                                <  SRR4302533.5831302/94‑1 (MQ=60)
                              CCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACG                                                                <  SRR4302533.628420/94‑1 (MQ=60)
                                AGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCG                                                              >  SRR4302533.1310976/1‑94 (MQ=60)
                                AGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCG                                                              <  SRR4302533.3467511/94‑1 (MQ=60)
                                  ATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCC                                                            >  SRR4302533.4908547/1‑94 (MQ=60)
                                   TCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCG                                                           <  SRR4302533.3232592/94‑1 (MQ=60)
                                   TCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCG                                                           <  SRR4302533.1509644/94‑1 (MQ=60)
                                    CCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGC                                                          >  SRR4302533.2694472/1‑94 (MQ=60)
                                    CCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGC                                                          >  SRR4302533.4431940/1‑94 (MQ=60)
                                    CCACAACTGCTGGCTGAGTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGC                                                          <  SRR4302533.5631252/94‑1 (MQ=60)
                                     CACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCT                                                         >  SRR4302533.2401689/1‑94 (MQ=60)
                                      ACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTT                                                        >  SRR4302533.4956855/1‑94 (MQ=60)
                                       CAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTC                                                       >  SRR4302533.5562475/1‑94 (MQ=60)
                                       CAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTC                                                       >  SRR4302533.3151754/1‑94 (MQ=60)
                                          CTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCG                                                    <  SRR4302533.5550137/94‑1 (MQ=60)
                                            GCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGAT                                                  <  SRR4302533.1039377/94‑1 (MQ=60)
                                            GCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGAT                                                  <  SRR4302533.6060130/94‑1 (MQ=60)
                                             CTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATA                                                 <  SRR4302533.1075650/94‑1 (MQ=60)
                                             CTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATA                                                 <  SRR4302533.1929233/94‑1 (MQ=60)
                                               GGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAAC                                               >  SRR4302533.211113/1‑94 (MQ=60)
                                                GCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCAGGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACG                                              <  SRR4302533.4464738/94‑1 (MQ=60)
                                                 CTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGC                                             <  SRR4302533.3995155/94‑1 (MQ=60)
                                                  TGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCA                                            >  SRR4302533.1365098/1‑94 (MQ=60)
                                                   GATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAG                                           <  SRR4302533.847273/94‑1 (MQ=60)
                                                   GATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAG                                           >  SRR4302533.4865276/1‑94 (MQ=60)
                                                     TTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAA                                         <  SRR4302533.4710212/94‑1 (MQ=60)
                                                     TTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAA                                         >  SRR4302533.474286/1‑94 (MQ=60)
                                                      TACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAAC                                        <  SRR4302533.3460266/94‑1 (MQ=60)
                                                      TACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAAC                                        >  SRR4302533.2152111/1‑94 (MQ=60)
                                                       ACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACT                                       >  SRR4302533.3887436/1‑94 (MQ=60)
                                                       ACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACT                                       <  SRR4302533.772355/94‑1 (MQ=60)
                                                         CGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGC                                     >  SRR4302533.5225921/1‑94 (MQ=60)
                                                         CGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGC                                     >  SRR4302533.2643663/1‑94 (MQ=60)
                                                         CGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGC                                     >  SRR4302533.5618141/1‑94 (MQ=60)
                                                         CGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGC                                     >  SRR4302533.5648478/1‑94 (MQ=60)
                                                            AGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATA                                  >  SRR4302533.842594/1‑94 (MQ=60)
                                                            AGCCTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATA                                  >  SRR4302533.4971523/1‑94 (MQ=60)
                                                               CTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCG                               >  SRR4302533.6076678/1‑94 (MQ=60)
                                                               CTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCG                               <  SRR4302533.562733/94‑1 (MQ=60)
                                                               CTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCG                               <  SRR4302533.1057031/94‑1 (MQ=60)
                                                               CTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCG                               <  SRR4302533.562732/94‑1 (MQ=60)
                                                               CTGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCG                               <  SRR4302533.3810180/94‑1 (MQ=60)
                                                                TGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGA                              >  SRR4302533.1106945/1‑94 (MQ=60)
                                                                TGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGA                              >  SRR4302533.2983712/1‑94 (MQ=60)
                                                                TGAAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGA                              <  SRR4302533.5759753/94‑1 (MQ=60)
                                                                  AAAGGAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAG                            <  SRR4302533.3660696/94‑1 (MQ=60)
                                                                      GAATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAA                        >  SRR4302533.3884383/1‑94 (MQ=60)
                                                                       AATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAAC                       >  SRR4302533.2138742/1‑94 (MQ=60)
                                                                       AATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAAC                       <  SRR4302533.2350380/94‑1 (MQ=60)
                                                                       AATAGTAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAAC                       <  SRR4302533.5827792/94‑1 (MQ=60)
                                                                            TAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACG                  <  SRR4302533.18108/94‑1 (MQ=60)
                                                                            TAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACG                  <  SRR4302533.2972277/94‑1 (MQ=60)
                                                                            TAATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACG                  >  SRR4302533.588931/1‑94 (MQ=60)
                                                                              ATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTG                >  SRR4302533.6003177/1‑94 (MQ=60)
                                                                              ATGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTG                >  SRR4302533.1602463/1‑94 (MQ=60)
                                                                               TGTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGT               <  SRR4302533.44721/94‑1 (MQ=60)
                                                                                GTCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGTT              >  SRR4302533.4574845/1‑94 (MQ=60)
                                                                                 TCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTT             <  SRR4302533.956588/94‑1 (MQ=60)
                                                                                 TCATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTT             <  SRR4302533.4442470/94‑1 (MQ=60)
                                                                                  CATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTG            >  SRR4302533.5654366/1‑94 (MQ=60)
                                                                                  CATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTG            <  SRR4302533.6145976/94‑1 (MQ=60)
                                                                                  CATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTG            >  SRR4302533.2120994/1‑94 (MQ=60)
                                                                                   ATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTGT           >  SRR4302533.6036133/1‑94 (MQ=60)
                                                                                   ATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTGT           >  SRR4302533.4870512/1‑94 (MQ=60)
                                                                                   ATTAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTGT           >  SRR4302533.2983785/1‑94 (MQ=60)
                                                                                     TAATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTGTCT         <  SRR4302533.2919804/94‑1 (MQ=60)
                                                                                      AATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTGTCTG        <  SRR4302533.5216804/94‑1 (MQ=60)
                                                                                      AATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTGTCTG        >  SRR4302533.6031110/1‑94 (MQ=60)
                                                                                       ATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTGTCTGG       >  SRR4302533.2854897/1‑94 (MQ=60)
                                                                                       ATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTGTCTGG       <  SRR4302533.6058880/94‑1 (MQ=60)
                                                                                       ATCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTGTCTGG       >  SRR4302533.225448/1‑94 (MQ=60)
                                                                                        TCAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTGTCTGGT      <  SRR4302533.1651905/94‑1 (MQ=60)
                                                                                         CAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTGTCTGGTG     >  SRR4302533.4349025/1‑94 (MQ=60)
                                                                                         CAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTGTCTGGTG     >  SRR4302533.2434443/1‑94 (MQ=60)
                                                                                         CAGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTGTCTGGTG     >  SRR4302533.3578188/1‑94 (MQ=60)
                                                                                          AGCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTGTCTGGTGG    >  SRR4302533.802765/1‑94 (MQ=60)
                                                                                           GCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTGTCTGGTGGG   <  SRR4302533.1354127/94‑1 (MQ=60)
                                                                                           GCATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTGTCTGGTGGG   <  SRR4302533.5085915/94‑1 (MQ=60)
                                                                                            CATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTGTCTGGTGGGC  >  SRR4302533.1961166/1‑94 (MQ=60)
                                                                                            CATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTGTCTGGTGGGC  >  SRR4302533.4025860/1‑94 (MQ=60)
                                                                                            CATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTGTCTGGTGGGC  <  SRR4302533.149265/94‑1 (MQ=60)
                                                                                            CATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTGTCTGGTGGGC  <  SRR4302533.1162349/94‑1 (MQ=60)
                                                                                            |                                                                                             
AACGCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGTATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAACTGCATATCGAAGATAACGAACGTGTTTGTCTGGTGGGC  >  NC_000913/1009109‑1009294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑