Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,017,437 T→C D29G (GAC→GGC)  b0955 ← putative ATP‑dependent protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,017,4370TC99.7% 964.4 / NA 302D29G (GAC→GGC) b0955putative ATP‑dependent protease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (169/132);  minor base A (1/0);  total (170/132)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 325 total aligned reads displayed.

ATAAAAGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTT  >  NC_000913/1017347‑1017526
                                                                                          |                                                                                         
aTAAAAGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGcca                                                                                         >  2:7084729/1‑93 (MQ=255)
aTAAAAGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGcca                                                                                         >  2:914311/1‑93 (MQ=255)
 tAAAAGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGccaa                                                                                        <  1:6018189/93‑1 (MQ=255)
 tAAAAGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGccaa                                                                                        >  1:7144291/1‑93 (MQ=255)
 tAAAAGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGccaa                                                                                        <  2:1144856/93‑1 (MQ=255)
 tAAAAGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGccaa                                                                                        >  1:3249854/1‑93 (MQ=255)
   aaaGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGccaatc                                                                                      <  2:3169411/93‑1 (MQ=255)
      gaggagGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCaaa                                                                                   <  2:9445448/93‑1 (MQ=255)
      gaggagGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCaaa                                                                                   <  1:1792857/93‑1 (MQ=255)
       aggaggATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAAt                                                                                  <  2:8153020/93‑1 (MQ=255)
        ggaggaTGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAAtg                                                                                 >  1:7628633/1‑93 (MQ=255)
         gaggaTGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAAtgt                                                                                <  2:8830147/93‑1 (MQ=255)
         gaggaTGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAAtgt                                                                                >  2:1437904/1‑93 (MQ=255)
          aggaTGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAAtgtg                                                                               <  2:5106990/93‑1 (MQ=255)
           ggaTGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTgg                                                                              >  2:3269957/1‑93 (MQ=255)
            gaTGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGc                                                                             <  2:1863228/93‑1 (MQ=255)
            gaTGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGc                                                                             >  1:5635563/1‑93 (MQ=255)
             aTGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCt                                                                            >  1:4834256/1‑93 (MQ=255)
              tGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTg                                                                           >  1:2736795/1‑93 (MQ=255)
              tGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTg                                                                           >  1:2873828/1‑93 (MQ=255)
                cTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAg                                                                         <  1:8235756/93‑1 (MQ=255)
                cTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAg                                                                         >  1:4492046/1‑93 (MQ=255)
                   gcgTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCaa                                                                      >  1:2159816/1‑93 (MQ=255)
                   gcgTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCaa                                                                      >  2:4243370/1‑93 (MQ=255)
                    cgTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCaaa                                                                     <  1:5312766/93‑1 (MQ=255)
                     gTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAat                                                                    >  1:4905449/1‑93 (MQ=255)
                     gTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAat                                                                    >  1:8366973/1‑93 (MQ=255)
                      tATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAata                                                                   >  1:8883517/1‑93 (MQ=255)
                      tATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAata                                                                   >  2:1555059/1‑93 (MQ=255)
                      tATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAata                                                                   >  2:5447133/1‑93 (MQ=255)
                      tATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGACAATCAAATGTGGCTGAGCAAatt                                                                   >  1:1406775/1‑92 (MQ=255)
                       aTGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAatat                                                                  >  1:3094353/1‑93 (MQ=255)
                        tGCAGCAACTGCTCCAGCGCAAATCGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATAtt                                                                 >  2:2452935/1‑93 (MQ=255)
                           agcagcTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTcc                                                              <  2:2099389/93‑1 (MQ=255)
                           agcaACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTcc                                                              <  1:9076016/93‑1 (MQ=255)
                            gcaACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCt                                                             <  2:6368330/93‑1 (MQ=255)
                            gcaACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCt                                                             >  2:3950377/1‑93 (MQ=255)
                             caACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATAGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTg                                                            >  1:7721196/1‑93 (MQ=255)
                              aaCTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTTCTGa                                                           >  2:1742681/1‑93 (MQ=255)
                              aaCTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGa                                                           <  2:7192729/93‑1 (MQ=255)
                                cTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATa                                                         <  1:2623514/93‑1 (MQ=255)
                                cTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATa                                                         >  1:4682521/1‑93 (MQ=255)
                                cTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATa                                                         <  2:845688/93‑1 (MQ=255)
                                   cTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCt                                                      >  1:2050327/1‑93 (MQ=255)
                                   cTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCt                                                      <  2:1344452/93‑1 (MQ=255)
                                    tCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTa                                                     <  1:61755/93‑1 (MQ=255)
                                       aGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCg                                                  >  2:8388113/1‑93 (MQ=255)
                                        gcgcAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCgg                                                 >  1:5157077/1‑93 (MQ=255)
                                        gcgcAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAAAGTGGCTGAGCAAATATTTCCTGATAGCTATCgg                                                 >  1:9696919/1‑93 (MQ=255)
                                         cgcAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGt                                                <  1:5658934/93‑1 (MQ=255)
                                           cAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTAt                                              >  2:3037431/1‑93 (MQ=255)
                                             aaTTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCa                                            <  2:2420751/93‑1 (MQ=255)
                                             aaTTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCa                                            >  2:2034258/1‑93 (MQ=255)
                                             aaTTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCa                                            >  1:7977636/1‑93 (MQ=255)
                                              aTTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAg                                           <  1:1366538/93‑1 (MQ=255)
                                                 gCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGACAGCTATCGGTATCAGGaa                                        <  2:5883639/93‑1 (MQ=255)
                                                  cAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAAc                                       <  2:6232077/93‑1 (MQ=255)
                                                    gCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCa                                     >  2:2313591/1‑93 (MQ=255)
                                                     ccGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAg                                    <  2:8621537/93‑1 (MQ=255)
                                                         ggTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCa                                <  2:3104542/93‑1 (MQ=255)
                                                          gTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCAc                               >  1:415183/1‑93 (MQ=255)
                                                          gTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCAc                               >  2:3334280/1‑93 (MQ=255)
                                                          gTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGAAAATATTTCCTGATAGCTATCGGTATCAGGAAGCAGGTCAc                               >  2:8175946/1‑93 (MQ=255)
                                                           ttGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACg                              >  1:8070314/1‑93 (MQ=255)
                                                                tATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATg                         >  1:7072015/1‑93 (MQ=255)
                                                                tATCACTGAATAAAGGATCGATTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATg                         <  2:2565750/93‑1 (MQ=255)
                                                                 aTCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGc                        >  1:8680474/1‑93 (MQ=255)
                                                                  tCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCa                       >  2:4577308/1‑93 (MQ=255)
                                                                  tCACAGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCa                       >  2:6491558/1‑93 (MQ=255)
                                                                     cTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGt                    >  2:4696207/1‑93 (MQ=255)
                                                                      tGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGtt                   <  2:8873955/93‑1 (MQ=255)
                                                                      tGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGtt                   <  2:3636772/93‑1 (MQ=255)
                                                                        aaTAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTc                 <  1:8581357/93‑1 (MQ=255)
                                                                        aaTAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTc                 >  2:6596721/1‑93 (MQ=255)
                                                                         aTAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCg                <  1:3731565/93‑1 (MQ=255)
                                                                          tAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGt               <  2:2192325/93‑1 (MQ=255)
                                                                          tAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGt               >  1:6544526/1‑93 (MQ=255)
                                                                          tAAAGGATCATTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGt               <  1:9978291/93‑1 (MQ=255)
                                                                           aaaGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCATGAACCAGGTCACGCCATGCAAGTTTCGTa              >  1:9492828/1‑93 (MQ=255)
                                                                           aaaGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTa              >  2:9765144/1‑93 (MQ=255)
                                                                           aaaGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTa              <  1:40799/93‑1 (MQ=255)
                                                                            aaGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTaa             <  1:1052226/93‑1 (MQ=255)
                                                                            aaGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTaa             <  1:173974/93‑1 (MQ=255)
                                                                            aaGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTaa             <  1:4041187/93‑1 (MQ=255)
                                                                            aaGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTaa             >  2:7646383/1‑93 (MQ=255)
                                                                             aGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAAt            <  1:5448273/93‑1 (MQ=255)
                                                                             aGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAAt            <  2:7384315/93‑1 (MQ=255)
                                                                             aGGAGCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAAt            <  2:7414088/93‑1 (MQ=255)
                                                                              ggATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATg           <  1:6527470/93‑1 (MQ=255)
                                                                              ggATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATg           >  1:5359990/1‑93 (MQ=255)
                                                                              ggATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATg           <  1:395998/93‑1 (MQ=255)
                                                                               gATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATgg          >  2:2876241/1‑93 (MQ=255)
                                                                                aTCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGt         <  1:6558140/93‑1 (MQ=255)
                                                                                aTCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGt         <  1:6792798/93‑1 (MQ=255)
                                                                                 tCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTc        >  2:5260802/1‑93 (MQ=255)
                                                                                 tCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTc        >  1:1645110/1‑93 (MQ=255)
                                                                                  cGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGCCACGCCATGCAAGTTTCGTAATGGTCa       >  1:2367302/1‑93 (MQ=255)
                                                                                     tttCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAg    >  2:4095628/1‑93 (MQ=255)
                                                                                       tCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGtt  <  1:1167536/93‑1 (MQ=255)
                                                                                       tCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGCATCAGGCACCTTGTCACCCCATGCAAGTTTCGTAATGGTCAAAGtt  >  2:1635184/1‑93 (MQ=255)
                                                                                          |                                                                                         
ATAAAAGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTT  >  NC_000913/1017347‑1017526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCATAAAAGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATG  >  NC_000913/1017344‑1017530
                                                                                             |                                                                                             
AGCATAAAAGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGC                                                                                               >  SRR4302540.2731166/1‑94 (MQ=60)
  CATAAAAGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCA                                                                                             <  SRR4302540.8022372/94‑1 (MQ=60)
   ATAAAAGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAA                                                                                            >  SRR4302540.7223892/1‑94 (MQ=60)
   ATAAAAGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAA                                                                                            >  SRR4302540.7223194/1‑94 (MQ=60)
   ATAAAAGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAA                                                                                            >  SRR4302540.3285250/1‑94 (MQ=60)
    TAAAAGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAAT                                                                                           <  SRR4302540.6084939/94‑1 (MQ=60)
       AAGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAA                                                                                        >  SRR4302540.4500431/1‑94 (MQ=60)
        AGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAA                                                                                       >  SRR4302540.7137980/1‑94 (MQ=60)
        AGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAA                                                                                       <  SRR4302540.9642246/94‑1 (MQ=60)
         GAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAAT                                                                                      <  SRR4302540.1232881/94‑1 (MQ=60)
         GAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAAT                                                                                      <  SRR4302540.2306193/94‑1 (MQ=60)
         GAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAAT                                                                                      <  SRR4302540.1812340/94‑1 (MQ=60)
          AGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATG                                                                                     <  SRR4302540.2377083/94‑1 (MQ=60)
          AGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATG                                                                                     <  SRR4302540.3364907/94‑1 (MQ=60)
          AGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATG                                                                                     >  SRR4302540.6411283/1‑94 (MQ=60)
          AGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATG                                                                                     >  SRR4302540.7714427/1‑94 (MQ=60)
           GGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGT                                                                                    >  SRR4302540.6626884/1‑94 (MQ=60)
           GGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGT                                                                                    <  SRR4302540.1831017/94‑1 (MQ=60)
             AGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGG                                                                                  >  SRR4302540.1588626/1‑94 (MQ=60)
              GGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGC                                                                                 >  SRR4302540.5697585/1‑94 (MQ=60)
               GATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCT                                                                                >  SRR4302540.4886044/1‑94 (MQ=60)
                ATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATGACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTG                                                                               >  SRR4302540.5752639/1‑94 (MQ=60)
                ATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTG                                                                               >  SRR4302540.2905071/1‑94 (MQ=60)
                ATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTG                                                                               <  SRR4302540.1886211/94‑1 (MQ=60)
                ATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTG                                                                               >  SRR4302540.2766334/1‑94 (MQ=60)
                ATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTG                                                                               <  SRR4302540.6409172/94‑1 (MQ=60)
                ATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTG                                                                               <  SRR4302540.4125056/94‑1 (MQ=60)
                  GCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAG                                                                             >  SRR4302540.2156667/1‑94 (MQ=60)
                  GCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAG                                                                             >  SRR4302540.2156911/1‑94 (MQ=60)
                  GCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAG                                                                             >  SRR4302540.1387365/1‑94 (MQ=60)
                  GCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAG                                                                             >  SRR4302540.4540747/1‑94 (MQ=60)
                  GCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAG                                                                             >  SRR4302540.254339/1‑94 (MQ=60)
                   CTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGC                                                                            <  SRR4302540.8331480/94‑1 (MQ=60)
                     CGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAA                                                                          >  SRR4302540.2183074/1‑94 (MQ=60)
                      GCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAA                                                                         >  SRR4302540.6968003/1‑94 (MQ=60)
                       CGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCACATGTGGCTGAGCAAAT                                                                        >  SRR4302540.9232594/1‑94 (MQ=60)
                       CGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAAT                                                                        >  SRR4302540.8464069/1‑94 (MQ=60)
                       CGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAAT                                                                        <  SRR4302540.5370932/94‑1 (MQ=60)
                       CGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAAT                                                                        >  SRR4302540.4958238/1‑94 (MQ=60)
                        GTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATA                                                                       >  SRR4302540.8986337/1‑94 (MQ=60)
                        GTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATA                                                                       >  SRR4302540.4652818/1‑94 (MQ=60)
                        GTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATA                                                                       <  SRR4302540.3918400/94‑1 (MQ=60)
                        GTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATA                                                                       >  SRR4302540.1525384/1‑94 (MQ=60)
                        GTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATA                                                                       <  SRR4302540.379003/94‑1 (MQ=60)
                        GTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGACAATCAAATGTGGCTGAGCAAATT                                                                       >  SRR4302540.1421924/1‑94 (MQ=60)
                         TATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATAT                                                                      >  SRR4302540.3128279/1‑94 (MQ=60)
                          ATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAgtctt                                                                     >  SRR4302540.7391575/1‑89 (MQ=60)
                          ATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATT                                                                     >  SRR4302540.1727497/1‑94 (MQ=60)
                           TGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTT                                                                    <  SRR4302540.6707698/94‑1 (MQ=60)
                           TGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTT                                                                    >  SRR4302540.5934210/1‑94 (MQ=60)
                            GCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTC                                                                   <  SRR4302540.4110874/94‑1 (MQ=60)
                             CAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCC                                                                  >  SRR4302540.7781899/1‑94 (MQ=60)
                              AGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCT                                                                 <  SRR4302540.5624698/94‑1 (MQ=60)
                              AGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCT                                                                 <  SRR4302540.9185023/94‑1 (MQ=60)
                              AGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCT                                                                 >  SRR4302540.2681003/1‑94 (MQ=60)
                               GCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATAGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTG                                                                >  SRR4302540.7810810/1‑94 (MQ=60)
                                CAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGA                                                               >  SRR4302540.7756374/1‑94 (MQ=60)
                                CAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGA                                                               <  SRR4302540.4100917/94‑1 (MQ=60)
                                 AACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGAT                                                              <  SRR4302540.5182567/94‑1 (MQ=60)
                                  ACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATA                                                             <  SRR4302540.2873897/94‑1 (MQ=60)
                                  ACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATA                                                             >  SRR4302540.6129897/1‑94 (MQ=60)
                                  ACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATA                                                             >  SRR4302540.4732612/1‑94 (MQ=60)
                                   CTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAG                                                            <  SRR4302540.2651884/94‑1 (MQ=60)
                                   CTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAG                                                            >  SRR4302540.6610913/1‑94 (MQ=60)
                                    TGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGC                                                           >  SRR4302540.5462864/1‑94 (MQ=60)
                                     GCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCT                                                          >  SRR4302540.2072400/1‑94 (MQ=60)
                                      CTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTA                                                         >  SRR4302540.1720161/1‑94 (MQ=60)
                                       TCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTAT                                                        <  SRR4302540.62380/94‑1 (MQ=60)
                                       TCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTAT                                                        >  SRR4302540.8162846/1‑94 (MQ=60)
                                       TCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTAT                                                        <  SRR4302540.9660593/94‑1 (MQ=60)
                                          AGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGG                                                     >  SRR4302540.5213078/1‑94 (MQ=60)
                                          AGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGG                                                     >  SRR4302540.4947852/1‑94 (MQ=60)
                                          AGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAAAGTGGCTGAGCAAATATTTCCTGATAGCTATCGG                                                     >  SRR4302540.9810935/1‑94 (MQ=60)
                                           GCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGT                                                    >  SRR4302540.3878398/1‑94 (MQ=60)
                                            CGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTA                                                   <  SRR4302540.1408020/94‑1 (MQ=60)
                                            CGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTA                                                   <  SRR4302540.5721248/94‑1 (MQ=60)
                                             tcctgctgaaccgctcttccgatctagcgcttTAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTAT                                                  <  SRR4302540.1110568/62‑1 (MQ=60)
                                             GCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTAT                                                  <  SRR4302540.7545400/94‑1 (MQ=60)
                                             GCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTAT                                                  >  SRR4302540.8448122/1‑94 (MQ=60)
                                              CAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATC                                                 >  SRR4302540.488048/1‑94 (MQ=60)
                                              CAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATC                                                 >  SRR4302540.303316/1‑94 (MQ=60)
                                               AAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCCAATATTTCCTCATAGCTCTCGGTATCG                                                >  SRR4302540.7917599/1‑94 (MQ=60)
                                               AAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCA                                                >  SRR4302540.8070570/1‑94 (MQ=60)
                                               AAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCA                                                <  SRR4302540.8236587/94‑1 (MQ=60)
                                               AAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCA                                                >  SRR4302540.7917596/1‑94 (MQ=60)
                                               AAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCA                                                <  SRR4302540.4046324/94‑1 (MQ=60)
                                                AATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAG                                               >  SRR4302540.6909638/1‑94 (MQ=60)
                                                 ATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGG                                              <  SRR4302540.1381002/94‑1 (MQ=60)
                                                  TTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGA                                             <  SRR4302540.1500950/94‑1 (MQ=60)
                                                  TTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGA                                             >  SRR4302540.1150090/1‑94 (MQ=60)
                                                   TGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAA                                            >  SRR4302540.3272765/1‑94 (MQ=60)
                                                   TGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAA                                            <  SRR4302540.8149861/94‑1 (MQ=60)
                                                     CAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACC                                          >  SRR4302540.6135323/1‑94 (MQ=60)
                                                     CAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACC                                          >  SRR4302540.4492679/1‑94 (MQ=60)
                                                      AGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCA                                         >  SRR4302540.8172014/1‑94 (MQ=60)
                                                       GCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAG                                        <  SRR4302540.874429/94‑1 (MQ=60)
                                                        CCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGG                                       <  SRR4302540.838458/94‑1 (MQ=60)
                                                         CGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGT                                      >  SRR4302540.4682949/1‑94 (MQ=60)
                                                          GCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTC                                     <  SRR4302540.3764274/94‑1 (MQ=60)
                                                          GCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTC                                     <  SRR4302540.10012484/94‑1 (MQ=60)
                                                           CGGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCA                                    >  SRR4302540.4078964/1‑94 (MQ=60)
                                                            GGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCAC                                   >  SRR4302540.5377703/1‑94 (MQ=60)
                                                            GGTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCAC                                   >  SRR4302540.419531/1‑94 (MQ=60)
                                                             GTTGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACG                                  >  SRR4302540.8163950/1‑94 (MQ=60)
                                                               TGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCC                                <  SRR4302540.9427934/94‑1 (MQ=60)
                                                                GAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCA                               >  SRR4302540.6321681/1‑94 (MQ=60)
                                                                GAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCA                               >  SRR4302540.4135571/1‑94 (MQ=60)
                                                                GAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCA                               <  SRR4302540.849204/94‑1 (MQ=60)
                                                                GAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCA                               <  SRR4302540.5533015/94‑1 (MQ=60)
                                                                GAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCA                               >  SRR4302540.5076597/1‑94 (MQ=60)
                                                                 AGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCAT                              >  SRR4302540.2978086/1‑94 (MQ=60)
                                                                  GTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATG                             >  SRR4302540.8004462/1‑94 (MQ=60)
                                                                  GTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATG                             >  SRR4302540.7150687/1‑94 (MQ=60)
                                                                   TATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGC                            >  SRR4302540.8781286/1‑94 (MQ=60)
                                                                   TATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGC                            >  SRR4302540.4468894/1‑94 (MQ=60)
                                                                   TATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGC                            <  SRR4302540.4857236/94‑1 (MQ=60)
                                                                    ATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCA                           >  SRR4302540.5631949/1‑94 (MQ=60)
                                                                    ATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCA                           >  SRR4302540.7486517/1‑94 (MQ=60)
                                                                    ATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCA                           >  SRR4302540.2736090/1‑94 (MQ=60)
                                                                    ATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCA                           >  SRR4302540.8710447/1‑94 (MQ=60)
                                                                    ATCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCA                           <  SRR4302540.5358838/94‑1 (MQ=60)
                                                                     TCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAA                          <  SRR4302540.513784/94‑1 (MQ=60)
                                                                     TCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAA                          <  SRR4302540.9002891/94‑1 (MQ=60)
                                                                     TCACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGATCACGCCATGCAA                          <  SRR4302540.9002890/94‑1 (MQ=60)
                                                                      CACTGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAG                         >  SRR4302540.8623487/1‑94 (MQ=60)
                                                                         TGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTT                      >  SRR4302540.3186836/1‑94 (MQ=60)
                                                                         TGAATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTT                      <  SRR4302540.5397792/94‑1 (MQ=60)
                                                                           AATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCT                    >  SRR4302540.8820923/1‑94 (MQ=60)
                                                                           AATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCG                    <  SRR4302540.8681111/94‑1 (MQ=60)
                                                                            ATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGT                   <  SRR4302540.3772275/94‑1 (MQ=60)
                                                                            ATAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGT                   >  SRR4302540.6617142/1‑94 (MQ=60)
                                                                             TAAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCATGAACCAGGTCACGCCATGCAAGTTTCGTA                  >  SRR4302540.9605346/1‑94 (MQ=60)
                                                                             TAAAGGATCATTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTA                  <  SRR4302540.10094647/94‑1 (MQ=60)
                                                                              AAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAA                 <  SRR4302540.41219/94‑1 (MQ=60)
                                                                              AAAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAA                 <  SRR4302540.2828514/94‑1 (MQ=60)
                                                                               AAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAAT                >  SRR4302540.7173428/1‑94 (MQ=60)
                                                                               AAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAAT                <  SRR4302540.1062935/94‑1 (MQ=60)
                                                                               AAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAAT                <  SRR4302540.4084751/94‑1 (MQ=60)
                                                                               AAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAAT                >  SRR4302540.1771010/1‑94 (MQ=60)
                                                                               AAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAAT                >  SRR4302540.1771009/1‑94 (MQ=60)
                                                                               AAGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAAT                <  SRR4302540.175711/94‑1 (MQ=60)
                                                                                AGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATG               <  SRR4302540.5508226/94‑1 (MQ=60)
                                                                                AGGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATG               >  SRR4302540.5418865/1‑94 (MQ=60)
                                                                                 GGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGG              <  SRR4302540.6599960/94‑1 (MQ=60)
                                                                                 GGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGG              <  SRR4302540.400103/94‑1 (MQ=60)
                                                                                 GGATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGG              >  SRR4302540.2160675/1‑94 (MQ=60)
                                                                                  GATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGT             >  SRR4302540.1366320/1‑94 (MQ=60)
                                                                                   GTCGTTTGCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTC            <  SRR4302540.8657439/94‑1 (MQ=60)
                                                                                   ATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTC            >  SRR4302540.1663073/1‑94 (MQ=60)
                                                                                   ATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTC            <  SRR4302540.6630853/94‑1 (MQ=60)
                                                                                   ATCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTC            <  SRR4302540.6867928/94‑1 (MQ=60)
                                                                                    TCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCA           >  SRR4302540.9944391/1‑94 (MQ=60)
                                                                                    TCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCA           <  SRR4302540.5702277/94‑1 (MQ=60)
                                                                                    TCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCA           >  SRR4302540.5294948/1‑94 (MQ=60)
                                                                                    TCGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGCCACGCCATGCAAGTTTCGTAATGGTCA           >  SRR4302540.2392799/1‑94 (MQ=60)
                                                                                     CGTTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAA          <  SRR4302540.5720289/94‑1 (MQ=60)
                                                                                      GTTTTCGCTAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAA         >  SRR4302540.8818615/1‑94 (MQ=60)
                                                                                       TTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAG        <  SRR4302540.6711036/94‑1 (MQ=60)
                                                                                       TTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAG        >  SRR4302540.6609588/1‑94 (MQ=60)
                                                                                       TTTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAG        >  SRR4302540.2415359/1‑94 (MQ=60)
                                                                                        TTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGT       >  SRR4302540.4921912/1‑94 (MQ=60)
                                                                                        TTTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGT       <  SRR4302540.2882189/94‑1 (MQ=60)
                                                                                        TTTCGCAAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGT       <  SRR4302540.5479790/94‑1 (MQ=60)
                                                                                         TTCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTT      >  SRR4302540.9287143/1‑94 (MQ=60)
                                                                                          TCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTG     <  SRR4302540.1179636/94‑1 (MQ=60)
                                                                                          TCGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTG     >  SRR4302540.2117472/1‑94 (MQ=60)
                                                                                           CGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGA    >  SRR4302540.2641842/1‑94 (MQ=60)
                                                                                           CGCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGA    >  SRR4302540.8346758/1‑94 (MQ=60)
                                                                                            GCCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGAT   >  SRR4302540.2665591/1‑94 (MQ=60)
                                                                                             CCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATG  >  SRR4302540.8906559/1‑94 (MQ=60)
                                                                                             CCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATG  <  SRR4302540.7193861/94‑1 (MQ=60)
                                                                                             |                                                                                             
AGCATAAAAGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATG  >  NC_000913/1017344‑1017530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑