Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,056,882 T→C Y99C (TAC→TGC)  torR ← TorR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,056,8820TC99.5% 579.0 / ‑5.0 183Y99C (TAC→TGC) torRTorR
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (87/95);  total (87/96)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

Only 100 of 186 total aligned reads displayed.

TGCGGTTGAGCTTGTCGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGTAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACC‑AGAATAATCCCCACCGTTGAGCGTTCT  >  NC_000913/1056792‑1056971
                                                                                          |                                                                                          
tgCGGTTGAGCTTGTCGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCaa                                                                                          <  1:6120105/93‑1 (MQ=255)
   ggTTGAGCTTGTCGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCg                                                                                       <  2:5482259/93‑1 (MQ=255)
     ttGAGCTTGTCGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTc                                                                                     <  1:3539730/93‑1 (MQ=255)
      tGAGCTTTTCGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCt                                                                                    <  1:1797450/93‑1 (MQ=255)
      tGAGCTTGTCGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCt                                                                                    <  1:723464/93‑1 (MQ=255)
      tGAGCTTGTCGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCt                                                                                    <  1:878676/93‑1 (MQ=255)
        aGCTTGTCGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTgc                                                                                  <  2:1334858/93‑1 (MQ=255)
        aGCTTGTCGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTgc                                                                                  >  1:2454092/1‑93 (MQ=255)
         gCTTGTCGCGCGAGGTCGACTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTgcg                                                                                 >  1:2456879/1‑93 (MQ=255)
            tGTCGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGccc                                                                              >  2:3238903/1‑93 (MQ=255)
            tGTCGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGccc                                                                              <  2:3984900/93‑1 (MQ=255)
             gTCGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCa                                                                             <  1:1757909/93‑1 (MQ=255)
             gTCGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCa                                                                             >  2:2683933/1‑93 (MQ=255)
               cgcgcgAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCAtt                                                                           >  1:980297/1‑93 (MQ=255)
                gcgcgAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCAttt                                                                          >  1:2902094/1‑93 (MQ=255)
                   cgAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCa                                                                       <  1:5126254/93‑1 (MQ=255)
                   cgAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCa                                                                       >  2:3928768/1‑93 (MQ=255)
                    gAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAg                                                                      >  1:337436/1‑93 (MQ=255)
                       gTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGccc                                                                   >  1:221061/1‑93 (MQ=255)
                       gTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGccc                                                                   >  1:5335201/1‑93 (MQ=255)
                       gTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGccc                                                                   >  2:3561392/1‑93 (MQ=255)
                        tCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTTCAGCCca                                                                  >  1:3384274/1‑93 (MQ=255)
                         cGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCcaa                                                                 <  1:3975532/93‑1 (MQ=255)
                         cGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCcaa                                                                 <  1:3410038/93‑1 (MQ=255)
                          gATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCcaac                                                                <  2:3677146/93‑1 (MQ=255)
                          gATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCcaac                                                                >  1:1722839/1‑93 (MQ=255)
                            ttCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCcaacaa                                                              >  1:2028143/1‑93 (MQ=255)
                             tCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAAt                                                             <  1:2095809/93‑1 (MQ=255)
                             tCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAAt                                                             <  2:3350608/93‑1 (MQ=255)
                              cGCCAGAGCAGATTTTTCCCCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATa                                                            <  1:5956484/93‑1 (MQ=255)
                               gCCAGAGCATATTTTTCACCCGTACTACCAGTGCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCCCCCATTTCCAGCCCAACAATAc                                                           <  2:552616/93‑1 (MQ=255)
                               gCCAGAGCAGATTTTTCACCCGTACTCCCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATAc                                                           <  1:5926160/93‑1 (MQ=255)
                               gCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATAc                                                           >  2:3352856/1‑93 (MQ=255)
                                ccAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACg                                                          <  1:3595444/93‑1 (MQ=255)
                                 cAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCCACAATACgg                                                         >  1:4685253/1‑93 (MQ=255)
                                 cAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACgg                                                         <  1:4050768/93‑1 (MQ=255)
                                 cAGAGCAGATTTTTCACCCGAACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACgg                                                         >  2:289116/1‑93 (MQ=255)
                                  agagCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGt                                                        >  1:5591939/1‑93 (MQ=255)
                                  agagCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGt                                                        <  1:1540493/93‑1 (MQ=255)
                                     gCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCaa                                                     <  1:5692801/93‑1 (MQ=255)
                                     gCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCaa                                                     <  1:43827/93‑1 (MQ=255)
                                     gCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTGTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCaa                                                     <  1:2976915/93‑1 (MQ=255)
                                     acAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCaa                                                     <  2:4237808/92‑1 (MQ=255)
                                      cAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAAt                                                    <  2:1425129/93‑1 (MQ=255)
                                      cAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAAt                                                    <  2:1172942/93‑1 (MQ=255)
                                      cAGAGTTTTCTCCCGGACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAAt                                                    <  2:2005844/93‑1 (MQ=255)
                                       aGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATc                                                   >  1:4803294/1‑93 (MQ=255)
                                        gATTTTTCCCCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCGGCGCCCATTTCCAGCCCAACAATACGGTCAATcc                                                  <  2:594771/93‑1 (MQ=255)
                                        gATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATcc                                                  <  2:2154657/93‑1 (MQ=255)
                                        gATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATcc                                                  <  1:1912470/93‑1 (MQ=255)
                                        gATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATcc                                                  <  2:4670501/93‑1 (MQ=255)
                                           ttttCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGAt                                               <  1:5916419/93‑1 (MQ=255)
                                              tCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGc                                            <  2:1117774/93‑1 (MQ=255)
                                              tCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGc                                            <  2:2787857/93‑1 (MQ=255)
                                               cACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCt                                           <  1:835160/93‑1 (MQ=255)
                                                aCCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTg                                          >  2:2323252/1‑93 (MQ=255)
                                                aCCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTg                                          >  2:950951/1‑93 (MQ=255)
                                                 cccGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGc                                         >  1:5766966/1‑93 (MQ=255)
                                                 cccGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGc                                         >  1:4984977/1‑93 (MQ=255)
                                                  ccGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCg                                        >  1:5433313/1‑93 (MQ=255)
                                                   cGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGt                                       >  2:6197239/1‑93 (MQ=255)
                                                    gTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTc                                      <  1:4768855/93‑1 (MQ=255)
                                                    gTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTc                                      >  2:5691931/1‑93 (MQ=255)
                                                    gTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTc                                      <  1:3730455/93‑1 (MQ=255)
                                                     tactacCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTcc                                     >  1:4183363/1‑93 (MQ=255)
                                                       ctacCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCgg                                   <  1:5479522/93‑1 (MQ=255)
                                                       ctacCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCgg                                   <  1:5049539/93‑1 (MQ=255)
                                                         acCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTa                                 <  2:4471530/93‑1 (MQ=255)
                                                         acCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTa                                 >  1:3074035/1‑93 (MQ=255)
                                                          ccAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTaa                                >  1:990845/1‑93 (MQ=255)
                                                            aGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAAcc                              >  2:3013979/1‑93 (MQ=255)
                                                              gtCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCATCCCAACAATACGGTCAATCCGATCGCTGCGTCCCGTAACC‑Ag                           <  2:1872016/92‑1 (MQ=255)
                                                                   gcAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACC‑AGaataa                      >  1:3107045/1‑93 (MQ=255)
                                                                   gcAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACC‑AGaataa                      <  2:1414345/93‑1 (MQ=255)
                                                                    cAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACC‑AGaataat                     >  1:1734515/1‑93 (MQ=255)
                                                                     aGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACC‑AGAATAATc                    <  2:5778012/93‑1 (MQ=255)
                                                                     aGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACC‑AGAATAATc                    <  1:102191/93‑1 (MQ=255)
                                                                      gTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACC‑AGAATAATcc                   >  2:3821716/1‑93 (MQ=255)
                                                                      gTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACC‑AGAATAATcc                   >  2:4116873/1‑93 (MQ=255)
                                                                      gTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACC‑AGAATAATcc                   >  1:2153347/1‑93 (MQ=255)
                                                                       ttCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACC‑AGAATAATccc                  >  2:2475787/1‑93 (MQ=255)
                                                                        tCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACC‑AGAATAATcccc                 >  2:5396927/1‑93 (MQ=255)
                                                                        tCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACC‑AGAATAATcccc                 <  1:5879618/93‑1 (MQ=255)
                                                                         ccAGGGGTTTGGTGGCGCAATCGTCTGGGCCCATTTCCAGCCCAACAAAACGGGCAATCCGGCGCCTGGGTCCGGGAACCAAAAAAAATcccc                 >  2:1634333/1‑93 (MQ=255)
                                                                         ccAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACC‑AGAATAATCCCCa                <  1:461842/93‑1 (MQ=255)
                                                                          cAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACC‑AGAATAATCCCCAc               >  1:673290/1‑93 (MQ=255)
                                                                           aGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACC‑AGAATAATCCCCAcc              <  2:1802640/93‑1 (MQ=255)
                                                                           aGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACC‑AGAATAATCCCCAcc              >  2:507691/1‑93 (MQ=255)
                                                                               gTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACC‑AGAATAATCCCCACCGTTg          >  2:5632243/1‑93 (MQ=255)
                                                                               gTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACC‑AGAATAATCCCCACCGTTg          >  1:1000042/1‑93 (MQ=255)
                                                                               gTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACC‑AGAATAATCCCCACCGTTg          >  1:4296479/1‑93 (MQ=255)
                                                                               gTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACC‑AGAATAATCCCCACCGTTg          >  1:4348694/1‑93 (MQ=255)
                                                                               gTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACC‑AGAATAATCCCCACCGTTg          <  1:4572443/93‑1 (MQ=255)
                                                                               gTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACC‑AGAATAATCCCCACCGTTg          <  1:5338469/93‑1 (MQ=255)
                                                                               gTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGGAACC‑AGAATAATCCGCACCGTTg          >  1:1493385/1‑93 (MQ=255)
                                                                               gTTTAGTGACGCAATCGTCTCCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACC‑AGAATAATCCCCACCGTTg          <  2:1311779/93‑1 (MQ=255)
                                                                                tttGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGATAACC‑AGAATAATCCCCACCGTTGa         >  1:1103026/1‑93 (MQ=255)
                                                                                   ggTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACC‑AGAATAATCCCCACCGTTGAGCg      >  1:5157245/1‑93 (MQ=255)
                                                                                    gTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACC‑AGAATAATCCCCACCGTTGAGCGt     <  2:4373482/93‑1 (MQ=255)
                                                                                    gTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACC‑AGAATAATCCCCACCGTTGAGCGt     >  1:2714728/1‑93 (MQ=255)
                                                                                          |                                                                                          
TGCGGTTGAGCTTGTCGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGTAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACC‑AGAATAATCCCCACCGTTGAGCGTTCT  >  NC_000913/1056792‑1056971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGTGCGGTTGAGCTTGTCGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGTAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGAGCGTTCTCGCA  >  NC_000913/1056790‑1056975
                                                                                            |                                                                                             
GGTGCGGTTGAGCTTGTCGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCA                                                                                              <  SRR4302533.459291/94‑1 (MQ=60)
 GTGCGGTTGAGCTTGTCGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAA                                                                                             >  SRR4302533.4358408/1‑94 (MQ=60)
  TGCGGTTGAGCTTGTCGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAAT                                                                                            <  SRR4302533.6157011/94‑1 (MQ=60)
   GCGGTTGAGCTTGTCGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATC                                                                                           >  SRR4302533.554321/1‑94 (MQ=60)
     GGTTGAGCTTGTCGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGT                                                                                         >  SRR4302533.5334929/1‑94 (MQ=60)
     GGTTGAGCTTGTCGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGT                                                                                         >  SRR4302533.5950071/1‑94 (MQ=60)
     GGTTGAGCTTGTCGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGT                                                                                         >  SRR4302533.1743704/1‑94 (MQ=60)
      GTTGAGCTTGTCGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTC                                                                                        <  SRR4302533.5809854/94‑1 (MQ=60)
       TTGAGCTTGTCGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCT                                                                                       <  SRR4302533.3561542/94‑1 (MQ=60)
        TGAGCTTTTCGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTG                                                                                      <  SRR4302533.1808387/94‑1 (MQ=60)
        TGAGCTTGTCGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTG                                                                                      <  SRR4302533.727740/94‑1 (MQ=60)
        TGAGCTTGTCGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTG                                                                                      >  SRR4302533.5478773/1‑94 (MQ=60)
        TGAGCTTGTCGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTG                                                                                      <  SRR4302533.883834/94‑1 (MQ=60)
         GAGCTTGTCGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGC                                                                                     >  SRR4302533.2469237/1‑94 (MQ=60)
          AGCTTGTCGCGCGAGGTCGACTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCG                                                                                    >  SRR4302533.2472040/1‑94 (MQ=60)
            CTTGTCGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCC                                                                                  >  SRR4302533.1519748/1‑94 (MQ=60)
             TTGTCGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCATCTGCGCCC                                                                                 >  SRR4302533.5037636/1‑94 (MQ=60)
              TGTCGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCA                                                                                >  SRR4302533.480158/1‑94 (MQ=60)
               GTCGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCAT                                                                               <  SRR4302533.1768623/94‑1 (MQ=60)
                TCGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATT                                                                              >  SRR4302533.986045/1‑94 (MQ=60)
                 CGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTT                                                                             >  SRR4302533.2919922/1‑94 (MQ=60)
                  GCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTC                                                                            <  SRR4302533.6295686/94‑1 (MQ=60)
                     CGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAG                                                                         <  SRR4302533.5157223/94‑1 (MQ=60)
                     CGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAG                                                                         >  SRR4302533.339498/1‑94 (MQ=60)
                      GAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGC                                                                        >  SRR4302533.2028290/1‑94 (MQ=60)
                        GGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCC                                                                      >  SRR4302533.5367180/1‑94 (MQ=60)
                        GGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCC                                                                      >  SRR4302533.222446/1‑94 (MQ=60)
                         GTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTTCAGCCCA                                                                     >  SRR4302533.3405169/1‑94 (MQ=60)
                           CGATTCGCCAGAGCAGATTTTTCACCCGTACTACTAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAAC                                                                   <  SRR4302533.4694444/94‑1 (MQ=60)
                           CGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAAC                                                                   <  SRR4302533.3431099/94‑1 (MQ=60)
                           CGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAAC                                                                   <  SRR4302533.3999734/94‑1 (MQ=60)
                           CGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAAC                                                                   >  SRR4302533.1733286/1‑94 (MQ=60)
                            GATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACA                                                                  >  SRR4302533.5169658/1‑94 (MQ=60)
                             ATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACCA                                                                 >  SRR4302533.5141249/1‑94 (MQ=60)
                             ATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAA                                                                 >  SRR4302533.4405789/1‑94 (MQ=60)
                             ATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAA                                                                 >  SRR4302533.1673979/1‑94 (MQ=60)
                             ATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAA                                                                 >  SRR4302533.2040663/1‑94 (MQ=60)
                              TTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAAT                                                                <  SRR4302533.4292141/94‑1 (MQ=60)
                               TCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATA                                                               <  SRR4302533.2108714/94‑1 (MQ=60)
                               TCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATA                                                               <  SRR4302533.2939676/94‑1 (MQ=60)
                                CGCCAGAGCAGATTTTTCCCCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATAC                                                              <  SRR4302533.5992516/94‑1 (MQ=60)
                                 GCCAGAGCAGATTTTTCACCCGTACTCCCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACG                                                             <  SRR4302533.5961983/94‑1 (MQ=60)
                                 GCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACG                                                             <  SRR4302533.5961984/94‑1 (MQ=60)
                                  CCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCCACAATACGG                                                            >  SRR4302533.4713637/1‑94 (MQ=60)
                                  CCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGG                                                            <  SRR4302533.3617565/94‑1 (MQ=60)
                                   CAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGT                                                           <  SRR4302533.4075444/94‑1 (MQ=60)
                                   CAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGT                                                           <  SRR4302533.3109157/94‑1 (MQ=60)
                                   CAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGT                                                           >  SRR4302533.5625720/1‑94 (MQ=60)
                                    AGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTC                                                          <  SRR4302533.1549771/94‑1 (MQ=60)
                                       GCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAAT                                                       <  SRR4302533.44127/94‑1 (MQ=60)
                                       GCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAAT                                                       <  SRR4302533.5727181/94‑1 (MQ=60)
                                       GCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTGTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAAT                                                       <  SRR4302533.2995128/94‑1 (MQ=60)
                                        CAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATC                                                      >  SRR4302533.4832385/1‑94 (MQ=60)
                                          GATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCG                                                    <  SRR4302533.1924138/94‑1 (MQ=60)
                                          GATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCG                                                    <  SRR4302533.2285976/94‑1 (MQ=60)
                                             TTTTCACCCGTACTCCCAGTTCCCGCAGTCCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCATCCCAACAATACGGTCAATCCGATC                                                 <  SRR4302533.3520721/94‑1 (MQ=60)
                                             TTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATC                                                 <  SRR4302533.5952176/94‑1 (MQ=60)
                                             TTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATC                                                 <  SRR4302533.733773/94‑1 (MQ=60)
                                                TCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCAAACAATACGGTCAATCCGATCGCT                                              <  SRR4302533.3531852/94‑1 (MQ=60)
                                                 CACCCGTACTACCAGTTCGCGCAGTTCTAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATACGATCGCTG                                             >  SRR4302533.2201769/1‑94 (MQ=60)
                                                 CACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTG                                             >  SRR4302533.3421155/1‑94 (MQ=60)
                                                 CACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTG                                             <  SRR4302533.840055/94‑1 (MQ=60)
                                                  ACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGC                                            >  SRR4302533.5801781/1‑94 (MQ=60)
                                                  ACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGC                                            >  SRR4302533.5015202/1‑94 (MQ=60)
                                                   CCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCG                                           >  SRR4302533.5466034/1‑94 (MQ=60)
                                                      GTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCC                                        >  SRR4302533.4208694/1‑94 (MQ=60)
                                                      GTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCC                                        <  SRR4302533.3753337/94‑1 (MQ=60)
                                                      GTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCC                                        <  SRR4302533.4797761/94‑1 (MQ=60)
                                                      GTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCC                                        <  SRR4302533.2677761/94‑1 (MQ=60)
                                                       TACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCG                                       <  SRR4302533.5787405/94‑1 (MQ=60)
                                                         CTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGT                                     <  SRR4302533.5080082/94‑1 (MQ=60)
                                                         CTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGT                                     <  SRR4302533.5512557/94‑1 (MQ=60)
                                                          TACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTA                                    <  SRR4302533.445276/94‑1 (MQ=60)
                                                          TACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTA                                    >  SRR4302533.3888467/1‑94 (MQ=60)
                                                          TACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTA                                    >  SRR4302533.3092905/1‑94 (MQ=60)
                                                           ACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAA                                   >  SRR4302533.996641/1‑94 (MQ=60)
                                                              AGTTCGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCA                                >  SRR4302533.1849049/1‑94 (MQ=60)
                                                                  CGCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAAT                            >  SRR4302533.2844820/1‑94 (MQ=60)
                                                                   GCGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATA                           <  SRR4302533.329212/94‑1 (MQ=60)
                                                                    CGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAA                          >  SRR4302533.3126141/1‑94 (MQ=60)
                                                                    CGCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAA                          >  SRR4302533.4012181/1‑94 (MQ=60)
                                                                     GCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAAT                         <  SRR4302533.2084716/94‑1 (MQ=60)
                                                                     GCAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAAT                         >  SRR4302533.1745040/1‑94 (MQ=60)
                                                                      CAGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATC                        <  SRR4302533.989693/94‑1 (MQ=60)
                                                                       AGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCC                       <  SRR4302533.102827/94‑1 (MQ=60)
                                                                       AGTTCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCC                       >  SRR4302533.2166610/1‑94 (MQ=60)
                                                                          TCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCA                    <  SRR4302533.4903606/94‑1 (MQ=60)
                                                                          TCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCA                    <  SRR4302533.1235136/94‑1 (MQ=60)
                                                                          TCCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCA                    <  SRR4302533.5915153/94‑1 (MQ=60)
                                                                           CCAGCGGTTTGGTGACGTAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCAC                   <  SRR4302533.3608224/94‑1 (MQ=60)
                                                                           CCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCACTCCGATCGCTCCGTCCGGTAACCAGAATAATCCCCAC                   >  SRR4302533.1748117/1‑94 (MQ=60)
                                                                           CCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCAC                   <  SRR4302533.685413/94‑1 (MQ=60)
                                                                           CCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCAC                   >  SRR4302533.677265/1‑94 (MQ=60)
                                                                           CCAGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCAC                   <  SRR4302533.464597/94‑1 (MQ=60)
                                                                             AGCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCG                 >  SRR4302533.1077769/1‑94 (MQ=60)
                                                                              GCGGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGT                >  SRR4302533.6005053/1‑94 (MQ=60)
                                                                                GGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTG              >  SRR4302533.4322318/1‑94 (MQ=60)
                                                                                GGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTG              >  SRR4302533.4374911/1‑94 (MQ=60)
                                                                                GGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTG              >  SRR4302533.1005879/1‑94 (MQ=60)
                                                                                GGTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGGAACCAGAATAATCCGCACCGTTG              >  SRR4302533.1502389/1‑94 (MQ=60)
                                                                                 GTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGA             <  SRR4302533.4600151/94‑1 (MQ=60)
                                                                                 GTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGA             <  SRR4302533.5370479/94‑1 (MQ=60)
                                                                                 GTTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGATAACCAGAATAATCCCCACCGTTGA             >  SRR4302533.1109556/1‑94 (MQ=60)
                                                                                  TTTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGAG            <  SRR4302533.5705121/94‑1 (MQ=60)
                                                                                   TTGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGAGC           >  SRR4302533.2153120/1‑94 (MQ=60)
                                                                                    TGGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGAGCG          >  SRR4302533.5188381/1‑94 (MQ=60)
                                                                                     GGTGACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGAGCGT         >  SRR4302533.2731395/1‑94 (MQ=60)
                                                                                        GACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGAGCGTTCT      >  SRR4302533.5434572/1‑94 (MQ=60)
                                                                                        GACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGAGCGTTCT      <  SRR4302533.871785/94‑1 (MQ=60)
                                                                                        GACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGAGCGTTCT      >  SRR4302533.3638536/1‑94 (MQ=60)
                                                                                         ACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGAGCGTTCTC     <  SRR4302533.3047273/94‑1 (MQ=60)
                                                                                         ACGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGAGCGTTCTC     <  SRR4302533.4618733/94‑1 (MQ=60)
                                                                                          CGCAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGAGCGTTCTCG    >  SRR4302533.3605626/1‑94 (MQ=60)
                                                                                            CAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCTGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGAGCGTTCTCGCA  >  SRR4302533.4900154/1‑94 (MQ=60)
                                                                                            CAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGACTAATCCCCACCGTTGAGCGTTCTCGCA  >  SRR4302533.4622053/1‑94 (MQ=60)
                                                                                            CAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGAGCGTTCTCGCA  >  SRR4302533.114773/1‑94 (MQ=60)
                                                                                            CAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGAGCGTTCTCGCA  <  SRR4302533.5535236/94‑1 (MQ=60)
                                                                                            |                                                                                             
TGTGCGGTTGAGCTTGTCGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGTAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGAGCGTTCTCGCA  >  NC_000913/1056790‑1056975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑