Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,096,810 A→G noncoding (66/88 nt) serX ← tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,096,8100AG100.0% 267.6 / NA 91noncoding (66/88 nt)serXtRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (46/45);  total (46/45)

Only 100 of 109 total aligned reads displayed.

TCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTG  >  NC_000913/1096720‑1096898
                                                                                          |                                                                                        
tcATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAGcc                                                                                        >  2:685478/1‑93 (MQ=16)
tcATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGcc                                                                                        >  1:1647983/1‑93 (MQ=18)
  aTCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGcccc                                                                                      >  1:121438/1‑93 (MQ=25)
   tCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGccccc                                                                                     <  1:5539122/93‑1 (MQ=37)
    ccccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAGGCCCCg                                                                                    >  1:5646664/1‑93 (MQ=255)
    ccccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCg                                                                                    <  1:1346592/93‑1 (MQ=255)
     cccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGTGGTGAGGGGGGGATTCGAGCCCCCGa                                                                                   >  2:5092137/1‑93 (MQ=255)
     cccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGa                                                                                   <  1:2783794/93‑1 (MQ=255)
     cccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGACCCCGa                                                                                   >  2:5068547/1‑93 (MQ=255)
      ccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGaa                                                                                  >  1:4082392/1‑92 (MQ=255)
      ccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGAt                                                                                  <  1:934340/93‑1 (MQ=255)
       cccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGGCCCCGATa                                                                                 >  1:3902452/1‑93 (MQ=255)
       cccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATa                                                                                 <  1:2867698/93‑1 (MQ=255)
         cGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACg                                                                               >  1:4810600/1‑93 (MQ=255)
          ggTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAGACCCCGATACGt                                                                              >  2:3528410/1‑93 (MQ=255)
          ggTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGt                                                                              <  1:5533131/93‑1 (MQ=255)
           gtgtgtGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGtt                                                                             >  2:826503/1‑93 (MQ=255)
           gtgtgtGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGtt                                                                             <  2:2373333/93‑1 (MQ=255)
             gtgtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCTAGCCCCCGATACGTTGc                                                                           <  1:5431313/93‑1 (MQ=255)
             gtgtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGc                                                                           >  2:6218282/1‑93 (MQ=255)
              tgtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAGCCCCCGATAAGTTGcc                                                                          >  1:4023735/1‑93 (MQ=255)
              tgtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGcc                                                                          <  1:3255930/93‑1 (MQ=255)
              tgtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGcc                                                                          >  1:5867863/1‑93 (MQ=255)
              tgtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGCTGcc                                                                          >  2:5009307/1‑93 (MQ=255)
               gtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGCGGCCCCGATACGTTGCCg                                                                         >  1:5016592/1‑93 (MQ=14)
               gtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAGGCCCCGATACGTTGCCg                                                                         >  2:551380/1‑93 (MQ=255)
               gtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGCGAGGGGGGGGTTCGAGGCCCAGATACGTTGCCg                                                                         >  2:6014657/1‑93 (MQ=14)
                tgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAGCCCCCGATACGTTGGCGt                                                                        >  1:983969/1‑93 (MQ=255)
                tgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGt                                                                        <  2:554054/93‑1 (MQ=255)
                   aaTATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGtata                                                                     >  2:1981030/1‑93 (MQ=255)
                   aaTATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGtata                                                                     <  2:5708698/93‑1 (MQ=255)
                    atatACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATac                                                                    <  1:4065382/93‑1 (MQ=255)
                    atatACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATac                                                                    >  2:919670/1‑93 (MQ=255)
                     tataCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAGCCCCCGATACGTTGCCGTATaca                                                                   >  1:2235386/1‑93 (MQ=255)
                     tataCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATacc                                                                   >  1:728613/1‑92 (MQ=255)
                     tataCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATaca                                                                   >  2:87933/1‑93 (MQ=255)
                     tataCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATaca                                                                   <  2:5523381/93‑1 (MQ=255)
                       taCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAGCCCCCGATACGTTGCCGGATacaca                                                                 >  1:1194992/1‑93 (MQ=255)
                       taCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATacaca                                                                 <  1:5016098/93‑1 (MQ=255)
                       taCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATaaaca                                                                 >  2:6284554/1‑93 (MQ=255)
                        aCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATacacac                                                                >  1:6296420/1‑93 (MQ=255)
                          gAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTTCCGTATACACACtt                                                              >  2:944941/1‑93 (MQ=255)
                          gAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACtt                                                              >  2:1155610/1‑93 (MQ=255)
                          gAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACtt                                                              >  1:347494/1‑93 (MQ=37)
                             aaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTcc                                                           >  2:4371903/1‑93 (MQ=255)
                             aaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTcc                                                           >  1:5289303/1‑93 (MQ=255)
                              aaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCa                                                          >  2:3501294/1‑93 (MQ=255)
                              aaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCa                                                          <  1:2478643/93‑1 (MQ=37)
                              aaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCa                                                          <  1:3300548/93‑1 (MQ=37)
                               aaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAg                                                         <  1:6192783/93‑1 (MQ=37)
                               aaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAg                                                         <  1:2796259/93‑1 (MQ=37)
                               aaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAg                                                         <  1:5066113/93‑1 (MQ=37)
                                aaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAgg                                                        <  1:2871513/93‑1 (MQ=37)
                                aaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAgg                                                        >  1:3831214/1‑93 (MQ=255)
                                aaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAgg                                                        >  2:2789888/1‑93 (MQ=255)
                                aaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAgg                                                        >  2:1302077/1‑93 (MQ=255)
                                 aaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGc                                                       <  2:1723871/93‑1 (MQ=37)
                                  aGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCg                                                      >  2:3406494/1‑93 (MQ=37)
                                  aGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCg                                                      <  1:73503/93‑1 (MQ=37)
                                   ccccGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGt                                                     <  2:3629390/92‑1 (MQ=25)
                                    cccGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGAATCGAGGCCCCGATACCTTCGCGTATACACACTTTCCAGGtgtg                                                    >  2:4716052/1‑93 (MQ=11)
                                     ccGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGc                                                   >  1:1536272/1‑93 (MQ=21)
                                     ccGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGc                                                   >  1:205250/1‑93 (MQ=21)
                                     ccGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGc                                                   >  2:2311170/1‑93 (MQ=21)
                                      cGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGGGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCt                                                  <  2:1846856/93‑1 (MQ=16)
                                      cGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCt                                                  <  1:523066/93‑1 (MQ=18)
                                       gTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTc                                                 >  1:2988830/1‑93 (MQ=17)
                                        tACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTcc                                                <  2:1229426/93‑1 (MQ=14)
                                        tACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTcc                                                >  2:676596/1‑93 (MQ=14)
                                        tACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTcc                                                >  2:5069685/1‑93 (MQ=14)
                                                                        ggTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCg                <  1:3317725/93‑1 (MQ=17)
                                                                        ggTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCg                >  1:151989/1‑93 (MQ=17)
                                                                        ggTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCg                <  2:1100493/93‑1 (MQ=17)
                                                                        ggTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCCCCTTCAGCCACTCGGACACCTCACCAAATTGTCg                <  2:4667945/93‑1 (MQ=12)
                                                                         gTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGt               <  2:6211050/93‑1 (MQ=18)
                                                                         gTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGt               <  1:1516984/93‑1 (MQ=18)
                                                                           gAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTc             >  2:3423158/1‑93 (MQ=25)
                                                                           gAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTc             <  1:4218487/93‑1 (MQ=25)
                                                                             gggggggATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCt           <  1:5464837/93‑1 (MQ=255)
                                                                             ggggcggATTGGAGCCCCCGATACGTTGCCGTATACACGCTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCt           <  1:4482685/93‑1 (MQ=14)
                                                                               gggggATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGt         >  1:4757182/1‑93 (MQ=255)
                                                                               gggggATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGt         >  1:2940433/1‑93 (MQ=255)
                                                                               gggggATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGt         >  1:1265553/1‑93 (MQ=255)
                                                                               gggggATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGt         <  2:1296683/93‑1 (MQ=255)
                                                                               gggggATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGt         >  2:5467220/1‑93 (MQ=255)
                                                                                ggggATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTc        >  2:124359/1‑93 (MQ=255)
                                                                                ggggATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTc        >  1:5713717/1‑93 (MQ=255)
                                                                                ggggATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTc        <  2:499649/93‑1 (MQ=255)
                                                                                ggggATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTc        <  1:6015952/93‑1 (MQ=255)
                                                                                 gggATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCt       >  1:2191869/1‑93 (MQ=255)
                                                                                 gggATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCt       <  2:4678211/93‑1 (MQ=255)
                                                                                 gggATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCt       <  2:3800512/93‑1 (MQ=255)
                                                                                  ggATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCtt      <  1:3820733/93‑1 (MQ=255)
                                                                                   gATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTg     >  1:2676426/1‑93 (MQ=255)
                                                                                    aTTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGc    <  1:2049438/93‑1 (MQ=255)
                                                                                     ttCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCt   >  2:5324756/1‑93 (MQ=255)
                                                                                     ttCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCt   >  2:6000193/1‑93 (MQ=255)
                                                                                     ttCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCt   >  2:4768921/1‑93 (MQ=255)
                                                                                     ttCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCt   <  2:1431807/93‑1 (MQ=255)
                                                                                      tCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTg  >  1:5034197/1‑93 (MQ=255)
                                                                                          |                                                                                        
TCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTG  >  NC_000913/1096720‑1096898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACG  >  NC_000913/1096717‑1096903
                                                                                             |                                                                                             
TTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAG                                                                                               <  SRR4302533.5858830/94‑1 (MQ=5)
 TCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGC                                                                                              >  SRR4302533.4812962/1‑94 (MQ=5)
  CTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAGCC                                                                                             >  SRR4302533.4889623/1‑94 (MQ=0)
  CTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCC                                                                                             >  SRR4302533.1657965/1‑94 (MQ=5)
    CATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCC                                                                                           >  SRR4302533.122205/1‑94 (MQ=5)
      TCCCCCCGTTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCG                                                                                         <  SRR4302533.5572569/94‑1 (MQ=9)
      TCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAGGCCCCG                                                                                         >  SRR4302533.5680799/1‑94 (MQ=0)
       CCCCCCGGTGTGTTCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGA                                                                                        <  SRR4302533.1354695/94‑1 (MQ=11)
        CCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGAT                                                                                       <  SRR4302533.5729345/94‑1 (MQ=15)
        CCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGAT                                                                                       <  SRR4302533.2800895/94‑1 (MQ=15)
        CCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGAA                                                                                       >  SRR4302533.4107244/1‑94 (MQ=12)
         CCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGGCCCCGATA                                                                                      >  SRR4302533.3926284/1‑94 (MQ=2)
         CCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATA                                                                                      <  SRR4302533.939826/94‑1 (MQ=20)
          CCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATAC                                                                                     <  SRR4302533.2885341/94‑1 (MQ=23)
           CCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACG                                                                                    >  SRR4302533.4839757/1‑94 (MQ=25)
             GGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTT                                                                                  <  SRR4302533.5566538/94‑1 (MQ=30)
                GTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAGCCCCCGATAAGTTGCC                                                                               >  SRR4302533.4048231/1‑94 (MQ=18)
                GTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCTAGCCCCCGATACGTTGCC                                                                               <  SRR4302533.5464022/94‑1 (MQ=24)
                GTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCC                                                                               <  SRR4302533.4023568/94‑1 (MQ=38)
                GTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCC                                                                               >  SRR4302533.5903329/1‑94 (MQ=38)
                 TGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGCGGCCCCGATACGTTGCCG                                                                              >  SRR4302533.5046984/1‑94 (MQ=4)
                 TGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCG                                                                              <  SRR4302533.3276007/94‑1 (MQ=40)
                  GTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAGCCCCCGATACGTTGGCGT                                                                             >  SRR4302533.989737/1‑94 (MQ=26)
                       ATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAGCCCCCGATACGTTGCCGTATACA                                                                        >  SRR4302533.2249179/1‑94 (MQ=48)
                       ATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACC                                                                        >  SRR4302533.732916/1‑94 (MQ=37)
                       ATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACA                                                                        <  SRR4302533.4090130/94‑1 (MQ=38)
                         ATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAGCCCCCGATACGTTGCCGGATACACA                                                                      >  SRR4302533.1202175/1‑94 (MQ=30)
                          TACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACAC                                                                     >  SRR4302533.6333983/1‑94 (MQ=30)
                          TACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACAC                                                                     <  SRR4302533.5046486/94‑1 (MQ=30)
                            CGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTT                                                                   >  SRR4302533.349614/1‑94 (MQ=25)
                             GAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTT                                                                  >  SRR4302533.2296536/1‑94 (MQ=25)
                               AAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCC                                                                >  SRR4302533.5320981/1‑94 (MQ=25)
                                 AAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAG                                                              <  SRR4302533.2493929/94‑1 (MQ=25)
                                 AAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAG                                                              <  SRR4302533.3320926/94‑1 (MQ=25)
                                  AAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGG                                                             <  SRR4302533.6229944/94‑1 (MQ=25)
                                  AAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGG                                                             <  SRR4302533.2813455/94‑1 (MQ=25)
                                  AAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGG                                                             >  SRR4302533.3854635/1‑94 (MQ=25)
                                  AAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGG                                                             <  SRR4302533.5096712/94‑1 (MQ=25)
                                   AAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGC                                                            <  SRR4302533.2889181/94‑1 (MQ=23)
                                     AGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGT                                                          <  SRR4302533.73975/94‑1 (MQ=15)
                                       CCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGC                                                        >  SRR4302533.1545503/1‑94 (MQ=10)
                                       CCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGC                                                        >  SRR4302533.206553/1‑94 (MQ=10)
                                        CCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCT                                                       <  SRR4302533.3379018/94‑1 (MQ=7)
                                         CGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTC                                                      >  SRR4302533.3007114/1‑94 (MQ=5)
                                         CGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTC                                                      <  SRR4302533.526168/94‑1 (MQ=5)
                                            ACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACCCACCTTCCAGGCGTGCTCCTT                                                   >  SRR4302533.898956/1‑94 (MQ=0)
                                            ACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTT                                                   >  SRR4302533.5051314/1‑94 (MQ=0)
                                            ACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTT                                                   >  SRR4302533.3907877/1‑94 (MQ=0)
                                             CTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTC                                                  >  SRR4302533.2715150/1‑94 (MQ=0)
                                             CTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTC                                                  >  SRR4302533.248653/1‑94 (MQ=0)
                                              TTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCA                                                 <  SRR4302533.1784987/94‑1 (MQ=0)
                                               TTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAG                                                <  SRR4302533.2869203/94‑1 (MQ=0)
                                                 CGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCC                                              >  SRR4302533.1837278/1‑94 (MQ=0)
                                                  GTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCC                                             >  SRR4302533.3346828/1‑94 (MQ=0)
                                                  GTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCA                                             >  SRR4302533.4666391/1‑94 (MQ=0)
                                                  GTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTACAGACACTTTCCAGGGGTGCTCCTTCAGCCA                                             >  SRR4302533.1938507/1‑94 (MQ=0)
                                                   TACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCAC                                            <  SRR4302533.4832227/94‑1 (MQ=0)
                                                    ACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACT                                           <  SRR4302533.167130/94‑1 (MQ=0)
                                                     CGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTC                                          >  SRR4302533.2237298/1‑94 (MQ=0)
                                                      GAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCG                                         <  SRR4302533.6197940/94‑1 (MQ=0)
                                                      GAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCG                                         >  SRR4302533.1237431/1‑94 (MQ=0)
                                                      GAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCG                                         <  SRR4302533.4512189/94‑1 (MQ=0)
                                                       AGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGG                                        >  SRR4302533.6192919/1‑94 (MQ=0)
                                                       AGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGG                                        >  SRR4302533.670277/1‑94 (MQ=0)
                                                       AGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGG                                        >  SRR4302533.4009628/1‑94 (MQ=0)
                                                        GCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGA                                       >  SRR4302533.547304/1‑94 (MQ=0)
                                                        GCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGA                                       >  SRR4302533.4767589/1‑94 (MQ=0)
                                                        GCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGA                                       >  SRR4302533.453455/1‑94 (MQ=0)
                                                        GCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGA                                       >  SRR4302533.3594425/1‑94 (MQ=0)
                                                         CTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGAC                                      <  SRR4302533.5885056/94‑1 (MQ=0)
                                                         CTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGGGCTCCTTCAGCCACTCGGAC                                      >  SRR4302533.6172571/1‑94 (MQ=0)
                                                          TCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACA                                     >  SRR4302533.5392202/1‑94 (MQ=0)
                                                          TCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACA                                     >  SRR4302533.6102671/1‑94 (MQ=0)
                                                           CTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACAAACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACAC                                    >  SRR4302533.4109746/1‑94 (MQ=0)
                                                            TTCTTTAAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACC                                   >  SRR4302533.2658039/1‑94 (MQ=0)
                                                            TTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACC                                   <  SRR4302533.3963789/94‑1 (MQ=0)
                                                                TTAAATATGGCGGTGAGGGGGGGATTCGATCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCAC                               <  SRR4302533.4126424/94‑1 (MQ=0)
                                                                TTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCAC                               >  SRR4302533.4676027/1‑94 (MQ=0)
                                                                  AAATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCA                             >  SRR4302533.1911199/1‑94 (MQ=0)
                                                                  AAATATGGCGGGGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCA                             >  SRR4302533.1710236/1‑94 (MQ=0)
                                                                    ATATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAA                           >  SRR4302533.88675/1‑94 (MQ=0)
                                                                     TATGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAAT                          >  SRR4302533.2586942/1‑94 (MQ=0)
                                                                     TATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAAT                          <  SRR4302533.109533/94‑1 (MQ=0)
                                                                      ATGGCGGGGAGGGGGGTATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATT                         <  SRR4302533.6202598/94‑1 (MQ=0)
                                                                       TGGCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTG                        <  SRR4302533.5446963/94‑1 (MQ=0)
                                                                         GCGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTC                      <  SRR4302533.5279891/94‑1 (MQ=0)
                                                                          CGGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCG                     >  SRR4302533.152960/1‑94 (MQ=2)
                                                                           GGTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGT                    <  SRR4302533.3338217/94‑1 (MQ=5)
                                                                            GTGAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTT                   <  SRR4302533.1526117/94‑1 (MQ=7)
                                                                              GAGGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCC                 <  SRR4302533.4244002/94‑1 (MQ=12)
                                                                                GGGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTG               <  SRR4302533.5497770/94‑1 (MQ=20)
                                                                                GGGGCGGATTGGAGCCCCCGATACGTTGCCGTATACACGCTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTG               <  SRR4302533.4509830/94‑1 (MQ=18)
                                                                                 GGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGT              >  SRR4302533.4786047/1‑94 (MQ=23)
                                                                                 GGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGT              >  SRR4302533.2958393/1‑94 (MQ=23)
                                                                                 GGGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGT              >  SRR4302533.1273125/1‑94 (MQ=23)
                                                                                  GGGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTC             >  SRR4302533.5748227/1‑94 (MQ=25)
                                                                                   GGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCT            <  SRR4302533.6052406/94‑1 (MQ=28)
                                                                                   GGGGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCT            >  SRR4302533.2205376/1‑94 (MQ=28)
                                                                                     GGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTG          >  SRR4302533.2692838/1‑94 (MQ=33)
                                                                                     GGATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTG          <  SRR4302533.3844097/94‑1 (MQ=33)
                                                                                       ATTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCT        <  SRR4302533.2062081/94‑1 (MQ=38)
                                                                                        TTCGAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTG       >  SRR4302533.5064691/1‑94 (MQ=40)
                                                                                           GAGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAA    >  SRR4302533.4503746/1‑94 (MQ=49)
                                                                                            AGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAAC   >  SRR4302533.2575936/1‑94 (MQ=52)
                                                                                            AGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAAC   >  SRR4302533.6056940/1‑94 (MQ=52)
                                                                                            AGCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAAC   >  SRR4302533.2722612/1‑94 (MQ=52)
                                                                                             GCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACG  <  SRR4302533.5742839/94‑1 (MQ=55)
                                                                                             GCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACG  <  SRR4302533.4035881/94‑1 (MQ=55)
                                                                                             GCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACG  >  SRR4302533.4652115/1‑94 (MQ=55)
                                                                                             GCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACG  <  SRR4302533.831348/94‑1 (MQ=55)
                                                                                             GCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACG  <  SRR4302533.600678/94‑1 (MQ=55)
                                                                                             |                                                                                             
TTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACG  >  NC_000913/1096717‑1096903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑