Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,222,734 T→C intergenic (‑583/‑184) b1172 ← / → b1173 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,222,7340TC100.0% 688.8 / NA 213intergenic (‑583/‑184)b1172/b1173hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (105/108);  total (105/108)

Only 100 of 242 total aligned reads displayed.

TTATGCGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGTCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAGTCG  >  NC_000913/1222644‑1222823
                                                                                          |                                                                                         
ttATGCGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCctggcct                                                                                         <  1:6016716/93‑1 (MQ=255)
ttATGCGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCctggcct                                                                                         >  2:1499649/1‑93 (MQ=255)
 tATGCGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCctggcctg                                                                                        <  2:5504406/93‑1 (MQ=255)
  aTGCGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCcctggcctgg                                                                                       <  1:1551355/93‑1 (MQ=255)
  aTGCGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCcctggcctgg                                                                                       <  1:2018070/93‑1 (MQ=255)
   tGCGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCctggcctggt                                                                                      <  1:1103206/93‑1 (MQ=255)
   tGCGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCctggcctggt                                                                                      <  2:3125936/93‑1 (MQ=255)
     cGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGc                                                                                    >  2:4290980/1‑93 (MQ=255)
     cGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGc                                                                                    >  1:2982536/1‑93 (MQ=255)
     cGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGc                                                                                    >  2:2994836/1‑93 (MQ=255)
      gggATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGcc                                                                                   <  2:2490613/93‑1 (MQ=255)
        gATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTg                                                                                 >  2:1421038/1‑93 (MQ=255)
        gATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTg                                                                                 <  1:2118609/93‑1 (MQ=255)
        gATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTg                                                                                 <  2:530719/93‑1 (MQ=255)
         atatCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGc                                                                                <  2:2311937/93‑1 (MQ=255)
         atatCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGc                                                                                <  1:5903883/93‑1 (MQ=255)
         atatCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGc                                                                                <  2:6095355/93‑1 (MQ=255)
         atatCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGc                                                                                <  1:5196293/93‑1 (MQ=255)
          tatCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCg                                                                               <  2:4475364/93‑1 (MQ=255)
          tatCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCg                                                                               <  2:1038571/93‑1 (MQ=255)
           atcatcATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCgg                                                                              <  1:1756139/93‑1 (MQ=255)
             catcatTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGc                                                                            <  1:1918103/93‑1 (MQ=255)
             catcatTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGc                                                                            <  1:873530/93‑1 (MQ=255)
                catTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGa                                                                         <  1:4044090/93‑1 (MQ=255)
                 atTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATACCCTGGCCTGGTGCCTGCGGGCTGag                                                                        >  2:4592637/1‑92 (MQ=255)
                  tttCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATg                                                                       <  2:5351597/93‑1 (MQ=255)
                   ttCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGt                                                                      >  1:1360884/1‑93 (MQ=255)
                    tcatcaTGATGTCTTGGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAAGCCCCTGGCCTGGTGCCTGCGGGCTGATGtt                                                                     <  1:2783217/93‑1 (MQ=255)
                     catcatGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTg                                                                    >  1:2317034/1‑93 (MQ=255)
                     catcatGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTg                                                                    <  2:570730/93‑1 (MQ=255)
                       tcatGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTgca                                                                  >  2:3836965/1‑93 (MQ=255)
                        catGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTgcag                                                                 >  1:3370844/1‑93 (MQ=255)
                         atgatgTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTgcagc                                                                >  2:3477295/1‑93 (MQ=255)
                         atgatgTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTgcagc                                                                <  1:3114847/93‑1 (MQ=255)
                         atgatgTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTgcagc                                                                <  2:5885589/93‑1 (MQ=255)
                          tgatgTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTgcagca                                                               >  1:5575148/1‑93 (MQ=255)
                           gatgTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGcagcag                                                              <  2:4140827/93‑1 (MQ=255)
                           gatgTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGcagcag                                                              >  2:840354/1‑93 (MQ=255)
                             tgTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCagag                                                            >  1:764068/1‑93 (MQ=255)
                                cTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCtt                                                         >  1:1006785/1‑93 (MQ=255)
                                cTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCtt                                                         >  1:1485187/1‑93 (MQ=255)
                                 tttGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTa                                                        >  2:1054207/1‑93 (MQ=255)
                                 tttGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTa                                                        <  1:6137116/93‑1 (MQ=255)
                                 tttGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTa                                                        >  1:709960/1‑93 (MQ=255)
                                 tttGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTa                                                        <  1:1953370/93‑1 (MQ=255)
                                  ttGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGGGGGCTGATGTTGCAGCAGAGCTTAg                                                       <  2:3800135/93‑1 (MQ=255)
                                  ttGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAg                                                       >  1:3028903/1‑93 (MQ=255)
                                   tGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAgg                                                      <  2:4618626/93‑1 (MQ=255)
                                   tGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAgg                                                      <  2:341502/93‑1 (MQ=255)
                                       gAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCAc                                                  >  2:3263299/1‑93 (MQ=255)
                                        aGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACt                                                 <  1:460082/93‑1 (MQ=255)
                                         gCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACtt                                                <  2:2838534/93‑1 (MQ=255)
                                          cGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTa                                               <  2:1766030/93‑1 (MQ=255)
                                           ggTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAg                                              <  1:5112430/93‑1 (MQ=255)
                                           ggTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAg                                              >  2:1951092/1‑93 (MQ=255)
                                            gTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGa                                             <  2:4861732/93‑1 (MQ=255)
                                             tGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGAc                                            <  1:2558959/93‑1 (MQ=255)
                                                acacAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGct                                         >  2:5470099/1‑93 (MQ=255)
                                                acacAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGct                                         >  1:2155865/1‑93 (MQ=255)
                                                acacAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGct                                         <  1:2663637/93‑1 (MQ=255)
                                                  acaATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGctac                                       <  1:185549/93‑1 (MQ=255)
                                                  acaATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGctac                                       >  1:3707555/1‑93 (MQ=255)
                                                    aaTACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGctacta                                     <  1:356641/93‑1 (MQ=255)
                                                      tACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGTTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATc                                   <  1:165215/93‑1 (MQ=255)
                                                      tACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATc                                   <  2:2174502/93‑1 (MQ=255)
                                                      tACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATc                                   >  1:3047861/1‑93 (MQ=255)
                                                       acacCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCa                                  <  1:2892555/93‑1 (MQ=255)
                                                        cacCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCAt                                 >  2:4530481/1‑93 (MQ=255)
                                                        cacCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCAt                                 <  1:3647783/93‑1 (MQ=255)
                                                          ccTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCAttt                               >  2:1837884/1‑93 (MQ=255)
                                                           cTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTa                              <  2:2240452/93‑1 (MQ=255)
                                                            tGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTAt                             >  1:2440489/1‑93 (MQ=255)
                                                             gcgcTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATc                            <  1:516371/93‑1 (MQ=255)
                                                               gcTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCa                          <  1:5995262/93‑1 (MQ=255)
                                                               gcTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCa                          >  1:3344413/1‑93 (MQ=255)
                                                                cTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCaa                         <  1:4438949/93‑1 (MQ=255)
                                                                 tGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAAt                        <  2:5629005/93‑1 (MQ=255)
                                                                 tGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAAt                        >  2:4904822/1‑93 (MQ=255)
                                                                  gTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAACAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATg                       >  1:4219450/1‑93 (MQ=255)
                                                                   tctcTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATgg                      <  2:2021576/93‑1 (MQ=255)
                                                                    ctctTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGc                     <  2:295952/93‑1 (MQ=255)
                                                                     tctTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCa                    >  1:2447244/1‑93 (MQ=255)
                                                                       ttCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATg                  >  2:3983303/1‑93 (MQ=255)
                                                                         cAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGa                <  2:3653628/93‑1 (MQ=255)
                                                                          aGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGaa               <  2:3162100/93‑1 (MQ=255)
                                                                             aTGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCg            >  2:4832827/1‑93 (MQ=255)
                                                                              tGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGa           >  2:1337501/1‑93 (MQ=255)
                                                                               gAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGaa          >  2:771133/1‑93 (MQ=255)
                                                                                aaTCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGaaa         <  1:3191861/93‑1 (MQ=255)
                                                                                aaTCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGaaa         <  2:3336760/93‑1 (MQ=255)
                                                                                   ccccTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCa      >  2:1968636/1‑93 (MQ=255)
                                                                                   ccccTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCa      <  1:105185/93‑1 (MQ=255)
                                                                                    cccTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAg     >  1:6220150/1‑93 (MQ=255)
                                                                                    cccTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAg     >  2:639841/1‑93 (MQ=255)
                                                                                     cctggcctggTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAGt    <  1:145756/93‑1 (MQ=255)
                                                                                      ctggcctggtGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAGTc   <  1:3830941/93‑1 (MQ=255)
                                                                                      ctggcctggtGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAGTc   <  1:1282478/93‑1 (MQ=255)
                                                                                       tggcctggtGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGACATCAGTCg  >  1:6031777/1‑93 (MQ=255)
                                                                                       tggcctggtGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAGTCg  <  1:4965422/93‑1 (MQ=255)
                                                                                       tggcctggtGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAGTCg  <  1:1716440/93‑1 (MQ=255)
                                                                                          |                                                                                         
TTATGCGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGTCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAGTCG  >  NC_000913/1222644‑1222823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTCTTATGCGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGTCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAGTCGATAC  >  NC_000913/1222641‑1222827
                                                                                             |                                                                                             
TTCTTATGCGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGC                                                                                               <  SRR4302533.3351470/94‑1 (MQ=39)
 TCTTATGCGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCC                                                                                              >  SRR4302533.540703/1‑94 (MQ=39)
 TCTTATGCGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCC                                                                                              <  SRR4302533.3285166/94‑1 (MQ=39)
 TCTTATGCGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCC                                                                                              <  SRR4302533.4551084/94‑1 (MQ=39)
 TCTTATGCGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCC                                                                                              <  SRR4302533.3218250/94‑1 (MQ=39)
  CTTATGCGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCT                                                                                             >  SRR4302533.4531347/1‑94 (MQ=38)
   TTATGCGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTG                                                                                            <  SRR4302533.4195035/94‑1 (MQ=38)
   TTATGCGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTG                                                                                            <  SRR4302533.6053173/94‑1 (MQ=38)
     ATGCGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGT                                                                                          <  SRR4302533.1560698/94‑1 (MQ=38)
     ATGCGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGT                                                                                          <  SRR4302533.2030505/94‑1 (MQ=38)
     ATGCGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGT                                                                                          <  SRR4302533.1320544/94‑1 (MQ=38)
      TGCGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTG                                                                                         >  SRR4302533.2726070/1‑94 (MQ=38)
      TGCGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTG                                                                                         <  SRR4302533.1109737/94‑1 (MQ=38)
       GCGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGC                                                                                        >  SRR4302533.3000780/1‑94 (MQ=38)
       GCGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGC                                                                                        <  SRR4302533.1191038/94‑1 (MQ=38)
        CGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCC                                                                                       >  SRR4302533.1989896/1‑94 (MQ=35)
          GGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTG                                                                                     <  SRR4302533.1451820/94‑1 (MQ=30)
          GGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTG                                                                                     >  SRR4302533.2212666/1‑94 (MQ=30)
           GATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGC                                                                                    <  SRR4302533.2131676/94‑1 (MQ=30)
           GATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGC                                                                                    >  SRR4302533.3056699/1‑94 (MQ=30)
            ATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCG                                                                                   >  SRR4302533.6061944/1‑94 (MQ=30)
            ATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCG                                                                                   >  SRR4302533.1660375/1‑94 (MQ=30)
            ATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCG                                                                                   <  SRR4302533.5939538/94‑1 (MQ=30)
            ATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCG                                                                                   <  SRR4302533.5227630/94‑1 (MQ=30)
            ATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCG                                                                                   >  SRR4302533.3515413/1‑94 (MQ=30)
             TATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGG                                                                                  >  SRR4302533.5167847/1‑94 (MQ=33)
              ATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGG                                                                                 >  SRR4302533.2628134/1‑94 (MQ=35)
              ATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGG                                                                                 <  SRR4302533.1766837/94‑1 (MQ=35)
                CATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCT                                                                               <  SRR4302533.1928978/94‑1 (MQ=38)
                CATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCT                                                                               <  SRR4302533.1929812/94‑1 (MQ=38)
                CATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCT                                                                               <  SRR4302533.878660/94‑1 (MQ=38)
                 ATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTG                                                                              <  SRR4302533.1736086/94‑1 (MQ=38)
                 ATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTG                                                                              >  SRR4302533.1883210/1‑94 (MQ=38)
                  TCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGA                                                                             >  SRR4302533.1847814/1‑94 (MQ=38)
                   CATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGAT                                                                            <  SRR4302533.2056326/94‑1 (MQ=38)
                   CATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGAT                                                                            <  SRR4302533.4068704/94‑1 (MQ=38)
                     TTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGT                                                                          <  SRR4302533.4116159/94‑1 (MQ=38)
                     TTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGT                                                                          >  SRR4302533.1369060/1‑94 (MQ=38)
                       TCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTG                                                                        <  SRR4302533.2256225/94‑1 (MQ=38)
                       TCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTG                                                                        >  SRR4302533.2331379/1‑94 (MQ=38)
                       TCATCATGATGTCTTGGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAAGCCCCTGGCCTGGTGCCTGCGGGCTGATGTTG                                                                        <  SRR4302533.2800314/94‑1 (MQ=35)
                         ATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCA                                                                      >  SRR4302533.2461246/1‑94 (MQ=38)
                         ATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCA                                                                      <  SRR4302533.1733797/94‑1 (MQ=38)
                         ATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCA                                                                      <  SRR4302533.3955872/94‑1 (MQ=38)
                          TCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAG                                                                     >  SRR4302533.3391662/1‑94 (MQ=38)
                           CATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGC                                                                    >  SRR4302533.302202/1‑94 (MQ=38)
                            ATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCA                                                                   <  SRR4302533.3134000/94‑1 (MQ=38)
                            ATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCA                                                                   >  SRR4302533.5608842/1‑94 (MQ=38)
                            ATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCA                                                                   >  SRR4302533.5188482/1‑94 (MQ=38)
                               ATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAG                                                                >  SRR4302533.768569/1‑94 (MQ=45)
                               ATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAG                                                                <  SRR4302533.2802079/94‑1 (MQ=45)
                               ATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAG                                                                >  SRR4302533.3089919/1‑94 (MQ=45)
                                  TCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTT                                                             >  SRR4302533.1494151/1‑94 (MQ=53)
                                  TCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTT                                                             >  SRR4302533.1012676/1‑94 (MQ=53)
                                   CTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTA                                                            >  SRR4302533.714153/1‑94 (MQ=55)
                                    TTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAG                                                           <  SRR4302533.6174089/94‑1 (MQ=58)
                                    TTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAG                                                           <  SRR4302533.1965274/94‑1 (MQ=58)
                                    TTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAG                                                           >  SRR4302533.3047437/1‑94 (MQ=58)
                                     TTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGG                                                          <  SRR4302533.160724/94‑1 (MQ=58)
                                      TGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGT                                                         >  SRR4302533.3376471/1‑94 (MQ=58)
                                         TGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCAC                                                      <  SRR4302533.3700737/94‑1 (MQ=55)
                                         TGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCAC                                                      >  SRR4302533.2911150/1‑94 (MQ=55)
                                          GAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACT                                                     <  SRR4302533.2139050/94‑1 (MQ=58)
                                           AGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTT                                                    >  SRR4302533.5206028/1‑94 (MQ=60)
                                           AGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTT                                                    <  SRR4302533.2525746/94‑1 (MQ=60)
                                           AGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTT                                                    <  SRR4302533.462828/94‑1 (MQ=60)
                                             CGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAG                                                  <  SRR4302533.640847/94‑1 (MQ=60)
                                              GGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGA                                                 <  SRR4302533.5143324/94‑1 (MQ=60)
                                               GTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGAC                                                <  SRR4302533.1481206/94‑1 (MQ=60)
                                               GTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGAC                                                <  SRR4302533.743321/94‑1 (MQ=60)
                                                TGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACG                                               <  SRR4302533.2574693/94‑1 (MQ=60)
                                                TGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACG                                               <  SRR4302533.3317063/94‑1 (MQ=60)
                                                TGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACG                                               <  SRR4302533.5982583/94‑1 (MQ=60)
                                                  AACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCT                                             >  SRR4302533.2169138/1‑94 (MQ=60)
                                                   ACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTA                                            <  SRR4302533.2679988/94‑1 (MQ=60)
                                                   ACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTA                                            >  SRR4302533.4269484/1‑94 (MQ=60)
                                                    CACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTAC                                           >  SRR4302533.3730326/1‑94 (MQ=60)
                                                     ACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACT                                          <  SRR4302533.186701/94‑1 (MQ=60)
                                                      CAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCTGCAGAGCTTAGGTCACTTAGACGCTACTC                                         >  SRR4302533.6137751/1‑94 (MQ=60)
                                                       AATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTAT                                        <  SRR4302533.358814/94‑1 (MQ=60)
                                                        ATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATC                                       >  SRR4302533.3066520/1‑94 (MQ=60)
                                                         TACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGTTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCA                                      <  SRR4302533.166263/94‑1 (MQ=60)
                                                          ACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCAT                                     <  SRR4302533.2910335/94‑1 (MQ=60)
                                                           CACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATT                                    <  SRR4302533.3670211/94‑1 (MQ=60)
                                                           CACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATT                                    <  SRR4302533.3227637/94‑1 (MQ=60)
                                                            ACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTT                                   <  SRR4302533.5143047/94‑1 (MQ=60)
                                                             CCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTA                                  >  SRR4302533.1091669/1‑94 (MQ=60)
                                                              CTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTAT                                 >  SRR4302533.2455558/1‑94 (MQ=60)
                                                              CTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTAT                                 <  SRR4302533.6185335/94‑1 (MQ=60)
                                                              CTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTAT                                 >  SRR4302533.6053161/1‑94 (MQ=60)
                                                               TGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATC                                >  SRR4302533.3608368/1‑94 (MQ=60)
                                                               TGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATC                                <  SRR4302533.3796577/94‑1 (MQ=60)
                                                                GCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCC                               <  SRR4302533.519436/94‑1 (MQ=60)
                                                                 CGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCA                              <  SRR4302533.3159489/94‑1 (MQ=60)
                                                                 CGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCA                              >  SRR4302533.291824/1‑94 (MQ=60)
                                                                 CGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCA                              >  SRR4302533.3365077/1‑94 (MQ=60)
                                                                  GCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAA                             <  SRR4302533.6031572/94‑1 (MQ=60)
                                                                   CTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAAT                            <  SRR4302533.5241044/94‑1 (MQ=60)
                                                                   CTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAAT                            <  SRR4302533.4465780/94‑1 (MQ=60)
                                                                   CTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAAT                            >  SRR4302533.2727704/1‑94 (MQ=60)
                                                                   CTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAAT                            >  SRR4302533.6033135/1‑94 (MQ=60)
                                                                    TGTGTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGTGCTGATGTTGCAGCAGAGCTTAGGTCACTTATACGCTACTATCATTTATCCAATG                           <  SRR4302533.4688044/94‑1 (MQ=60)
                                                                    TGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATG                           >  SRR4302533.956977/1‑94 (MQ=60)
                                                                    TGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAACAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATG                           >  SRR4302533.4244967/1‑94 (MQ=60)
                                                                      TCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGC                         >  SRR4302533.2500747/1‑94 (MQ=60)
                                                                       CTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCA                        >  SRR4302533.2462346/1‑94 (MQ=60)
                                                                       CTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCA                        >  SRR4302533.995586/1‑94 (MQ=60)
                                                                        TCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCAT                       <  SRR4302533.1961057/94‑1 (MQ=60)
                                                                          TTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGG                     >  SRR4302533.1199881/1‑94 (MQ=60)
                                                                              GGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATC                 >  SRR4302533.849452/1‑94 (MQ=60)
                                                                               GATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCG                <  SRR4302533.4750919/94‑1 (MQ=60)
                                                                               GATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCG                >  SRR4302533.5390567/1‑94 (MQ=60)
                                                                                   AATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAAT            <  SRR4302533.3211496/94‑1 (MQ=60)
                                                                                   AATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAAT            >  SRR4302533.5612530/1‑94 (MQ=60)
                                                                                    ATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATC           <  SRR4302533.3076127/94‑1 (MQ=60)
                                                                                    ATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATC           >  SRR4302533.703675/1‑94 (MQ=60)
                                                                                      CCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAG         <  SRR4302533.105839/94‑1 (MQ=60)
                                                                                      CCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAG         >  SRR4302533.237956/1‑94 (MQ=60)
                                                                                      CCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAG         >  SRR4302533.6257439/1‑94 (MQ=60)
                                                                                      CCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAG         <  SRR4302533.1675557/94‑1 (MQ=60)
                                                                                      CCCCTGGCCTGGTGCCTGCGGGCTGATGGTGCAGCAGAGCTTAGGTCACGTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAG         <  SRR4302533.2063009/94‑1 (MQ=60)
                                                                                       CCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAGT        >  SRR4302533.6082124/1‑94 (MQ=60)
                                                                                        CCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAGTC       <  SRR4302533.146301/94‑1 (MQ=60)
                                                                                        CCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAGTC       >  SRR4302533.3442218/1‑94 (MQ=60)
                                                                                        CCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAGTC       <  SRR4302533.146686/94‑1 (MQ=60)
                                                                                         CTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGACATCAGTCG      >  SRR4302533.6068333/1‑94 (MQ=60)
                                                                                         CTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAGTCG      <  SRR4302533.1044263/94‑1 (MQ=60)
                                                                                         CTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAGTCG      <  SRR4302533.1290196/94‑1 (MQ=60)
                                                                                         CTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAGTCG      <  SRR4302533.155640/94‑1 (MQ=60)
                                                                                         CTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAGTCG      >  SRR4302533.3842277/1‑94 (MQ=60)
                                                                                         CTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAGTCG      <  SRR4302533.3854360/94‑1 (MQ=60)
                                                                                          TGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAGTCGA     <  SRR4302533.1726846/94‑1 (MQ=60)
                                                                                          TGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAGTCGA     >  SRR4302533.3332853/1‑94 (MQ=60)
                                                                                          TGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAGTCGA     <  SRR4302533.4995520/94‑1 (MQ=60)
                                                                                           GGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAGTCGAT    <  SRR4302533.4722990/94‑1 (MQ=60)
                                                                                             CCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAGTCGATAC  <  SRR4302533.2709229/94‑1 (MQ=60)
                                                                                             |                                                                                             
TTCTTATGCGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGTCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAGTCGATAC  >  NC_000913/1222641‑1222827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑