Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,280,531 A→G Y482C (TAC→TGC)  narG → nitrate reductase 1 alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,280,5310AG99.5% 661.3 / ‑4.8 207Y482C (TAC→TGC) narGnitrate reductase 1 alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (95/110);  total (96/111)
Fisher's exact test for biased strand distribution p-value = 4.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

Only 100 of 213 total aligned reads displayed.

TGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGATTA  >  NC_000913/1280441‑1280620
                                                                                          |                                                                                         
tgctggACAACCTGCCGGTGAAACTCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCg                                                                                         <  2:1842845/93‑1 (MQ=255)
tgctgcACAAACTGCCGGTGAACCGCCTGCAACTGGATGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCg                                                                                         <  2:4744770/93‑1 (MQ=255)
tgctgcACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCg                                                                                         >  2:4876180/1‑93 (MQ=255)
tgctgcACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCg                                                                                         >  2:1343771/1‑93 (MQ=255)
tgctgcACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCg                                                                                         >  1:2748776/1‑93 (MQ=255)
  ctgcACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGt                                                                                       >  1:784327/1‑93 (MQ=255)
   tgcACAAACTGCCGGTGAAACGCCTGCATCTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTc                                                                                      <  2:3484668/93‑1 (MQ=255)
   tgcACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTc                                                                                      <  1:1150932/93‑1 (MQ=255)
    gcACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCt                                                                                     >  1:3940336/1‑93 (MQ=255)
     cacaAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTg                                                                                    <  1:232148/93‑1 (MQ=255)
      acaAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTgg                                                                                   >  1:3292265/1‑93 (MQ=255)
       caAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGa                                                                                  <  1:5522248/93‑1 (MQ=255)
        aaaCTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGaa                                                                                 >  2:2314671/1‑93 (MQ=255)
        aaaCTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGaa                                                                                 <  1:2982953/93‑1 (MQ=255)
        aaaCTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGaa                                                                                 >  1:6214247/1‑93 (MQ=255)
         aaCTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAAc                                                                                <  1:3253587/93‑1 (MQ=255)
          aCTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACg                                                                               >  2:1114410/1‑93 (MQ=255)
            tGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTg                                                                             >  1:2355865/1‑93 (MQ=255)
             gCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACGGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGACCGTgg                                                                            <  2:1632427/93‑1 (MQ=255)
              ccGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGc                                                                           >  1:583307/1‑93 (MQ=255)
              ccGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGc                                                                           >  2:854786/1‑93 (MQ=255)
               cGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGcc                                                                          <  2:2840140/93‑1 (MQ=255)
               cGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGcc                                                                          >  2:4858229/1‑93 (MQ=255)
                ggTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTCTGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCt                                                                         <  1:4552570/93‑1 (MQ=255)
                ggTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCt                                                                         <  1:5114973/93‑1 (MQ=255)
                ggTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCt                                                                         >  2:6250827/1‑93 (MQ=255)
                  tGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGa                                                                       <  2:4464656/93‑1 (MQ=255)
                  tGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGa                                                                       <  1:2240965/93‑1 (MQ=255)
                  tGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGa                                                                       <  2:1968808/93‑1 (MQ=255)
                   gAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGaa                                                                      <  2:4948558/93‑1 (MQ=255)
                    aaaCGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAac                                                                     <  2:1487493/93‑1 (MQ=255)
                    aaaCGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAac                                                                     <  1:1927664/93‑1 (MQ=255)
                       cGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAacgac                                                                  <  2:324717/93‑1 (MQ=255)
                        gCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAacgacg                                                                 >  2:2247743/1‑93 (MQ=255)
                         ccTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGt                                                                >  1:1022099/1‑93 (MQ=255)
                          cTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGtt                                                               <  2:3859741/93‑1 (MQ=255)
                          cTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGtt                                                               >  1:4875027/1‑93 (MQ=255)
                           tGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTa                                                              >  2:1315029/1‑93 (MQ=255)
                            gCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTaa                                                             >  1:4331479/1‑93 (MQ=255)
                              aaCTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACt                                                           <  1:4991302/93‑1 (MQ=255)
                               aCTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACtg                                                          >  2:200608/1‑93 (MQ=255)
                                  ggCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGc                                                       <  2:1804098/93‑1 (MQ=255)
                                  ggCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGc                                                       >  1:3743006/1‑93 (MQ=255)
                                   gCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGCCGTTAACTGTGCa                                                      >  1:5660141/1‑93 (MQ=255)
                                      gATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCCAcc                                                   >  2:5942309/1‑93 (MQ=255)
                                      gATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAAcc                                                   >  1:656639/1‑93 (MQ=255)
                                        tGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGGTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAg                                                 >  2:5133349/1‑93 (MQ=255)
                                        tGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAg                                                 >  1:2902562/1‑93 (MQ=255)
                                        tGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAg                                                 >  2:741497/1‑93 (MQ=255)
                                         ggCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGc                                                <  1:376364/93‑1 (MQ=255)
                                          gcagcaCCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCt                                               <  1:418907/93‑1 (MQ=255)
                                           cagcaCCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTa                                              <  2:4327843/93‑1 (MQ=255)
                                           cagcaCCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTa                                              <  1:1722174/93‑1 (MQ=255)
                                             gcaCCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATg                                            >  2:3003313/1‑93 (MQ=255)
                                             gcaCCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATg                                            >  2:3431833/1‑93 (MQ=255)
                                              caCCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGa                                           <  1:997726/93‑1 (MQ=255)
                                                ccGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACg                                         >  2:1698314/1‑93 (MQ=255)
                                                   cccTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGAtg                                      <  1:3322875/93‑1 (MQ=255)
                                                    ccgggTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGACTGGAACGTGGCGTGAACGACGTTAACTGTGCAACCAGCTATGACGAtgt                                     <  2:619106/90‑1 (MQ=255)
                                                    ccTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGAtgt                                     <  1:2486979/93‑1 (MQ=255)
                                                    ccTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGAtgt                                     <  2:3549160/93‑1 (MQ=255)
                                                    ccTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGAtgt                                     >  2:6031998/1‑93 (MQ=255)
                                                     cTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGAtgtg                                    >  2:3335482/1‑93 (MQ=255)
                                                      tGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGa                                   >  1:97470/1‑93 (MQ=255)
                                                       ggTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGaa                                  >  1:3640582/1‑93 (MQ=255)
                                                        gTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGaaa                                 <  1:3597239/93‑1 (MQ=255)
                                                        gTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGaaa                                 <  1:1359673/93‑1 (MQ=255)
                                                        gTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGaaa                                 <  1:282223/93‑1 (MQ=255)
                                                         tGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAg                                <  1:4378810/93‑1 (MQ=255)
                                                            ccaccGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCtt                             >  2:2500263/1‑93 (MQ=255)
                                                            ccaccGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCtt                             <  1:3352597/93‑1 (MQ=255)
                                                            ccaccGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCtt                             >  1:2681515/1‑93 (MQ=255)
                                                            ccaccGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCtt                             >  1:723892/1‑93 (MQ=255)
                                                             caccGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTta                            <  1:83571/93‑1 (MQ=255)
                                                             caccGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTta                            >  1:1283819/1‑93 (MQ=255)
                                                             caccGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTta                            <  1:2834093/93‑1 (MQ=255)
                                                              accGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTtat                           >  1:305156/1‑93 (MQ=255)
                                                                cGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATAc                         <  1:6252570/93‑1 (MQ=255)
                                                                 gTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATAcc                        >  2:2901987/1‑93 (MQ=255)
                                                                  tttATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATAccc                       <  2:5618186/93‑1 (MQ=255)
                                                                  tttATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATAccc                       <  2:367204/93‑1 (MQ=255)
                                                                   ttATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATAcccc                      <  2:3799945/93‑1 (MQ=255)
                                                                     aTGATCTGACGCTGGCAAACTGCGGTCTGCAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCgg                    <  2:2728911/93‑1 (MQ=255)
                                                                       gATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGcc                  >  1:6009611/1‑93 (MQ=255)
                                                                       gATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGcc                  <  1:4753306/93‑1 (MQ=255)
                                                                        aTCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCt                 >  2:108884/1‑93 (MQ=255)
                                                                         tCTGACGCTGGCAAACTGCGGTCTGAAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTg                <  1:3027521/93‑1 (MQ=255)
                                                                           tGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTggg              >  1:1916803/1‑93 (MQ=255)
                                                                            gACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGc             <  2:1267268/93‑1 (MQ=255)
                                                                             aCGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGcc            >  1:3886423/1‑93 (MQ=255)
                                                                              cGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCg           <  2:4151534/93‑1 (MQ=255)
                                                                               gCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGa          >  2:5384623/1‑93 (MQ=255)
                                                                                cTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCgtg         >  2:5066613/1‑91 (MQ=255)
                                                                                cTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAg         <  2:792217/93‑1 (MQ=255)
                                                                                  ggCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCa       <  2:3923288/93‑1 (MQ=255)
                                                                                  ggCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCa       >  2:6167064/1‑93 (MQ=255)
                                                                                    cAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGa     <  1:1614331/93‑1 (MQ=255)
                                                                                     aaaCTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGAt    <  2:440512/93‑1 (MQ=255)
                                                                                     aaaCTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGAt    <  1:4662711/93‑1 (MQ=255)
                                                                                      aaCTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGAtt   >  1:5355514/1‑93 (MQ=255)
                                                                                          |                                                                                         
TGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGATTA  >  NC_000913/1280441‑1280620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGATTACCG  >  NC_000913/1280438‑1280623
                                                                                             |                                                                                            
TGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTG                                                                                              <  SRR4302533.6021386/94‑1 (MQ=60)
TGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTG                                                                                              <  SRR4302533.1736437/94‑1 (MQ=60)
 GCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGC                                                                                             >  SRR4302533.3516628/1‑94 (MQ=60)
  CTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCG                                                                                            <  SRR4302533.3986925/94‑1 (MQ=60)
  CTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCG                                                                                            >  SRR4302533.2765630/1‑94 (MQ=60)
  CTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCG                                                                                            <  SRR4302533.6294842/94‑1 (MQ=60)
   TGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGG                                                                                           <  SRR4302533.3683417/94‑1 (MQ=60)
    GCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGT                                                                                          <  SRR4302533.806477/94‑1 (MQ=60)
    GCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGT                                                                                          >  SRR4302533.788941/1‑94 (MQ=60)
     CTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTC                                                                                         <  SRR4302533.432701/94‑1 (MQ=60)
      TGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCT                                                                                        >  SRR4302533.3964380/1‑94 (MQ=60)
      TGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCT                                                                                        <  SRR4302533.1157827/94‑1 (MQ=60)
        CACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGG                                                                                      <  SRR4302533.391211/94‑1 (MQ=60)
        CACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGG                                                                                      >  SRR4302533.3312587/1‑94 (MQ=60)
        CACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGG                                                                                      <  SRR4302533.233582/94‑1 (MQ=60)
         ACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGA                                                                                     <  SRR4302533.3591104/94‑1 (MQ=60)
          CAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAA                                                                                    <  SRR4302533.5555592/94‑1 (MQ=60)
          CAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAA                                                                                    <  SRR4302533.1540759/94‑1 (MQ=60)
          CAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAA                                                                                    >  SRR4302533.6251512/1‑94 (MQ=60)
          CAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAA                                                                                    >  SRR4302533.692740/1‑94 (MQ=60)
           AAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAAC                                                                                   <  SRR4302533.3001200/94‑1 (MQ=60)
            AACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACG                                                                                  <  SRR4302533.3273651/94‑1 (MQ=60)
             ACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGT                                                                                 <  SRR4302533.5860164/94‑1 (MQ=60)
              CTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTG                                                                                >  SRR4302533.2370433/1‑94 (MQ=60)
               TGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGG                                                                               <  SRR4302533.6305561/94‑1 (MQ=60)
                GCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGC                                                                              >  SRR4302533.586747/1‑94 (MQ=60)
                 CCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCC                                                                             >  SRR4302533.2179596/1‑94 (MQ=60)
                   GGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTCTGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTG                                                                           <  SRR4302533.4580179/94‑1 (MQ=60)
                   GGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTG                                                                           >  SRR4302533.6269860/1‑94 (MQ=60)
                   GGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTG                                                                           <  SRR4302533.5145883/94‑1 (MQ=60)
                    GTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGA                                                                          >  SRR4302533.2842666/1‑94 (MQ=60)
                     ggcAACGCCTGCAACTGGCTGATGGCAGCACCGCTCTGGTGACCACCGTTTAGGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAA                                                                         <  SRR4302533.1252244/91‑1 (MQ=60)
                     TGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAA                                                                         <  SRR4302533.2254816/94‑1 (MQ=60)
                      GAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAAC                                                                        <  SRR4302533.4754535/94‑1 (MQ=60)
                       AAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACG                                                                       <  SRR4302533.1939425/94‑1 (MQ=60)
                        AACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGA                                                                      >  SRR4302533.2031147/1‑94 (MQ=60)
                        AACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGA                                                                      >  SRR4302533.2365808/1‑94 (MQ=60)
                         ACGCATGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGAC                                                                     <  SRR4302533.3290087/94‑1 (MQ=60)
                           GCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGT                                                                   >  SRR4302533.1028106/1‑94 (MQ=60)
                            CCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTT                                                                  >  SRR4302533.4904564/1‑94 (MQ=60)
                             CTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGATCGTGGCCTGAACGACGATA                                                                 >  SRR4302533.5158625/1‑94 (MQ=60)
                             CTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTA                                                                 >  SRR4302533.6031948/1‑94 (MQ=60)
                              TGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAA                                                                >  SRR4302533.4357576/1‑94 (MQ=60)
                              TGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAA                                                                >  SRR4302533.667435/1‑94 (MQ=60)
                               GCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAAC                                                               <  SRR4302533.2957240/94‑1 (MQ=60)
                               GCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAAC                                                               <  SRR4302533.4030452/94‑1 (MQ=60)
                                CAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACT                                                              <  SRR4302533.2375713/94‑1 (MQ=60)
                                 AACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTG                                                             <  SRR4302533.5021563/94‑1 (MQ=60)
                                 AACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTG                                                             <  SRR4302533.6181001/94‑1 (MQ=60)
                                    TGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGC                                                          >  SRR4302533.3765963/1‑94 (MQ=60)
                                     GGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGCCGTTAACTGTGCA                                                         >  SRR4302533.5694343/1‑94 (MQ=60)
                                     GGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCA                                                         <  SRR4302533.4336209/94‑1 (MQ=60)
                                     GGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCA                                                         <  SRR4302533.352503/94‑1 (MQ=60)
                                        TGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACC                                                      >  SRR4302533.660513/1‑94 (MQ=60)
                                          ATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAG                                                    >  SRR4302533.2920390/1‑94 (MQ=60)
                                            GGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCT                                                  <  SRR4302533.378634/94‑1 (MQ=60)
                                             GCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTA                                                 <  SRR4302533.421415/94‑1 (MQ=60)
                                              CAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTAT                                                <  SRR4302533.1732618/94‑1 (MQ=60)
                                              CAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTCAACTGTGCAACCAGCTAT                                                >  SRR4302533.981484/1‑94 (MQ=60)
                                               AGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATG                                               >  SRR4302533.5050164/1‑94 (MQ=60)
                                                 CACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTTCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGAC                                             <  SRR4302533.3656622/94‑1 (MQ=60)
                                                 CACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGAC                                             <  SRR4302533.1003552/94‑1 (MQ=60)
                                                 CACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGAC                                             <  SRR4302533.2319903/94‑1 (MQ=60)
                                                  ACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACG                                            <  SRR4302533.4640754/94‑1 (MQ=60)
                                                   CCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGA                                           >  SRR4302533.3875782/1‑94 (MQ=60)
                                                      CCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGT                                        <  SRR4302533.3343396/94‑1 (MQ=60)
                                                       CCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTG                                       <  SRR4302533.2502299/94‑1 (MQ=60)
                                                       CCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTG                                       <  SRR4302533.2640032/94‑1 (MQ=60)
                                                        CTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGA                                      >  SRR4302533.98077/1‑94 (MQ=60)
                                                         TGGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAA                                     >  SRR4302533.3662968/1‑94 (MQ=60)
                                                          GGTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAA                                    >  SRR4302533.4408583/1‑94 (MQ=60)
                                                           GTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAG                                   <  SRR4302533.283950/94‑1 (MQ=60)
                                                           GTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAG                                   <  SRR4302533.3619373/94‑1 (MQ=60)
                                                           GTGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAG                                   <  SRR4302533.1367844/94‑1 (MQ=60)
                                                            TGACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGC                                  <  SRR4302533.4405247/94‑1 (MQ=60)
                                                              ACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTT                                >  SRR4302533.728170/1‑94 (MQ=60)
                                                              ACCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTT                                >  SRR4302533.2697946/1‑94 (MQ=60)
                                                               CCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTA                               >  SRR4302533.1146014/1‑94 (MQ=60)
                                                               CCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTA                               >  SRR4302533.1291543/1‑94 (MQ=60)
                                                               CCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTA                               <  SRR4302533.3373309/94‑1 (MQ=60)
                                                               CCACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTA                               <  SRR4302533.1306143/94‑1 (MQ=60)
                                                                CACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTAT                              <  SRR4302533.2851540/94‑1 (MQ=60)
                                                                CACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTAT                              >  SRR4302533.3771660/1‑94 (MQ=60)
                                                                CACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTAT                              >  SRR4302533.307020/1‑94 (MQ=60)
                                                                CACCGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTAT                              <  SRR4302533.84102/94‑1 (MQ=60)
                                                                   CGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACC                           <  SRR4302533.6289970/94‑1 (MQ=60)
                                                                   CGTTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACC                           >  SRR4302533.4395998/1‑94 (MQ=60)
                                                                     TTTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCC                         <  SRR4302533.771663/94‑1 (MQ=60)
                                                                      TTATGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCG                        <  SRR4302533.5686070/94‑1 (MQ=60)
                                                                         TGATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCC                     >  SRR4302533.6046020/1‑94 (MQ=60)
                                                                          GATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCT                    >  SRR4302533.4800288/1‑94 (MQ=60)
                                                                          GATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCT                    <  SRR4302533.4782122/94‑1 (MQ=60)
                                                                          GATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCT                    <  SRR4302533.2714485/94‑1 (MQ=60)
                                                                           ATCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTG                   <  SRR4302533.3154888/94‑1 (MQ=60)
                                                                            TCTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGG                  <  SRR4302533.1930163/94‑1 (MQ=60)
                                                                            TCTGACGCTGGCAAACTGCGGTCTGAAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGG                  <  SRR4302533.3046048/94‑1 (MQ=60)
                                                                             CTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGG                 >  SRR4302533.1640573/1‑94 (MQ=60)
                                                                             CTGACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGG                 >  SRR4302533.1928504/1‑94 (MQ=60)
                                                                               GACGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCC               >  SRR4302533.3910133/1‑94 (MQ=60)
                                                                                 CGCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGA             >  SRR4302533.3361133/1‑94 (MQ=60)
                                                                                  GCTGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAG            <  SRR4302533.5302015/94‑1 (MQ=60)
                                                                                    TGGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCA          >  SRR4302533.3036943/1‑94 (MQ=60)
                                                                                     GGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAG         <  SRR4302533.5458812/94‑1 (MQ=60)
                                                                                     GGCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAG         <  SRR4302533.4923197/94‑1 (MQ=60)
                                                                                      GCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGA        <  SRR4302533.1878615/94‑1 (MQ=60)
                                                                                      GCAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGA        <  SRR4302533.3317618/94‑1 (MQ=60)
                                                                                       CAAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGAT       <  SRR4302533.1624116/94‑1 (MQ=60)
                                                                                        AAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGATT      <  SRR4302533.2897385/94‑1 (MQ=60)
                                                                                        AAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGATT      <  SRR4302533.2885685/94‑1 (MQ=60)
                                                                                        AAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGATT      <  SRR4302533.4690965/94‑1 (MQ=60)
                                                                                        AAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGATT      >  SRR4302533.5387663/1‑94 (MQ=60)
                                                                                        AAACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGATT      >  SRR4302533.278633/1‑94 (MQ=60)
                                                                                         AACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGATTA     <  SRR4302533.3065132/94‑1 (MQ=60)
                                                                                         AACTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGATTA     >  SRR4302533.890538/1‑94 (MQ=60)
                                                                                           CTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGATTACC   >  SRR4302533.2321770/1‑94 (MQ=60)
                                                                                           CTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGATTACC   >  SRR4302533.4684625/1‑94 (MQ=60)
                                                                                           CTGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGATTACC   <  SRR4302533.3581099/94‑1 (MQ=60)
                                                                                            TGCGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGATTACCG  <  SRR4302533.320793/94‑1 (MQ=60)
                                                                                             |                                                                                            
TGCTGCTGCACAAACTGCCGGTGAAACGCCTGCAACTGGCTGATGGCAGCACCGCCCTGGTGACCACCGTTTATGATCTGACGCTGGCAAACTACGGTCTGGAACGTGGCCTGAACGACGTTAACTGTGCAACCAGCTATGACGATGTGAAAGCTTATACCCCGGCCTGGGCCGAGCAGATTACCG  >  NC_000913/1280438‑1280623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑