Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,288,760 C→T P111L (CCG→CTG)  ychK → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,288,7600CT99.5% 757.6 / ‑4.0 204P111L (CCG→CTG) ychKhypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (101/102);  minor base G (1/0);  total (102/102)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.63e-01

Only 100 of 209 total aligned reads displayed.

GGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCCGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTGAAGGC  >  NC_000913/1288670‑1288848
                                                                                          |                                                                                        
 ggATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGa                                                                                       <  2:4431026/93‑1 (MQ=255)
     gTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACa                                                                                   >  2:1984445/1‑93 (MQ=255)
     gTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACa                                                                                   <  2:2371166/93‑1 (MQ=255)
     gTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACa                                                                                   >  2:5628291/1‑93 (MQ=255)
     gTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACa                                                                                   >  2:5889590/1‑93 (MQ=255)
      ttttACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACag                                                                                  <  2:5821763/93‑1 (MQ=255)
       tttACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACaga                                                                                 <  1:5050070/93‑1 (MQ=255)
         tACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAgaga                                                                               <  2:4359595/93‑1 (MQ=255)
         tACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAgaga                                                                               <  1:1328764/93‑1 (MQ=255)
           cGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATc                                                                             >  1:5667022/1‑93 (MQ=255)
            gCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCg                                                                            <  2:6249748/93‑1 (MQ=255)
            gCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCg                                                                            <  2:463953/93‑1 (MQ=255)
             tcTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGa                                                                           >  2:1637991/2‑93 (MQ=255)
              cTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGaa                                                                          <  1:2697394/93‑1 (MQ=255)
               tGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGaaa                                                                         >  1:243232/1‑93 (MQ=255)
               tGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGaaa                                                                         >  2:3970732/1‑93 (MQ=255)
                    gATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGt                                                                    >  1:429563/1‑93 (MQ=255)
                      tctctcCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTc                                                                  <  1:3516957/93‑1 (MQ=255)
                      tctctcCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTc                                                                  <  2:3777454/93‑1 (MQ=255)
                       ctctcCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTcc                                                                 <  2:1438305/93‑1 (MQ=255)
                       ctctcCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTcc                                                                 <  2:6205270/93‑1 (MQ=255)
                       ctctcCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTcc                                                                 >  1:3129885/1‑93 (MQ=255)
                       ctctcCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTcc                                                                 >  1:1124535/1‑93 (MQ=255)
                        tctcCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTccc                                                                <  1:5850153/93‑1 (MQ=255)
                        tctcCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTccc                                                                <  1:2144718/93‑1 (MQ=255)
                        tctcCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTccc                                                                <  1:4049221/93‑1 (MQ=255)
                        tctcCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTccc                                                                <  1:1055351/93‑1 (MQ=255)
                          tcCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCgc                                                              >  1:6219263/1‑92 (MQ=255)
                           ccTGGCAGCGCGGTGGGGTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTc                                                             >  1:632819/1‑93 (MQ=255)
                               gCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCtt                                                         <  2:2449962/93‑1 (MQ=255)
                                cAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCttt                                                        >  1:258437/1‑93 (MQ=255)
                                 aGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTg                                                       >  2:5871591/1‑93 (MQ=255)
                                  gcgcGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGc                                                      <  2:2208293/93‑1 (MQ=255)
                                  gcgcGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGc                                                      <  1:5221293/93‑1 (MQ=255)
                                     cgGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGc                                                   <  2:4456684/93‑1 (MQ=255)
                                     cgGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGc                                                   >  2:5053011/1‑93 (MQ=255)
                                     cgGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGc                                                   <  1:84640/93‑1 (MQ=255)
                                      ggTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCt                                                  >  2:5774854/1‑93 (MQ=255)
                                      ggTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCt                                                  >  1:1572406/1‑93 (MQ=255)
                                        tGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGt                                                <  1:4779033/93‑1 (MQ=255)
                                         gggTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGtt                                               <  2:2622430/93‑1 (MQ=255)
                                         gggTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGtt                                               <  1:2826517/93‑1 (MQ=255)
                                            ttccTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGcc                                            <  2:6083122/90‑1 (MQ=255)
                                            ttACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGcc                                            <  2:3042621/93‑1 (MQ=255)
                                              aCTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGccac                                          <  2:3344615/93‑1 (MQ=255)
                                              aCTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGccac                                          >  1:4503260/1‑93 (MQ=255)
                                              aCTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGATGTTGccac                                          >  2:2296330/1‑93 (MQ=255)
                                                 gcgcGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCaa                                       <  1:2274814/93‑1 (MQ=255)
                                                  cgcgGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCTGCTGTTGCCACCAAt                                      <  2:4231238/93‑1 (MQ=255)
                                                   gcggcgAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAAtt                                     >  2:4008863/1‑93 (MQ=255)
                                                     ggcgAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTa                                   >  1:5289061/1‑93 (MQ=255)
                                                     ggcgAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTa                                   >  1:1875931/1‑93 (MQ=255)
                                                        gAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGACAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGt                                <  1:4446386/93‑1 (MQ=255)
                                                        gAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGt                                >  2:1411756/1‑93 (MQ=255)
                                                         aGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTa                               >  1:3763530/1‑93 (MQ=255)
                                                          gCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTAc                              >  2:3585983/1‑93 (MQ=255)
                                                          gCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTAc                              >  2:4117485/1‑93 (MQ=255)
                                                          gCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTAc                              >  2:1004252/1‑93 (MQ=255)
                                                          gCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTAc                              >  2:1787721/1‑93 (MQ=255)
                                                          gCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTAc                              >  2:1052949/1‑93 (MQ=255)
                                                          gCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTAc                              >  1:68599/1‑93 (MQ=255)
                                                           cGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACg                             <  1:2865540/93‑1 (MQ=255)
                                                           cGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACg                             <  1:5071230/93‑1 (MQ=255)
                                                            gtgtCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACgg                            <  2:3000178/93‑1 (MQ=255)
                                                             tgtCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACggg                           >  1:574982/1‑93 (MQ=255)
                                                              gtCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGa                          >  1:111602/1‑93 (MQ=255)
                                                               tCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGAc                         >  1:923030/1‑93 (MQ=255)
                                                               tCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGAc                         >  1:1764370/1‑93 (MQ=255)
                                                               tCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGAc                         >  1:78570/1‑93 (MQ=255)
                                                               tCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGAc                         <  1:986074/93‑1 (MQ=255)
                                                               tCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGAc                         >  1:3395403/1‑93 (MQ=255)
                                                                cTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACg                        >  2:3111562/1‑93 (MQ=255)
                                                                cTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACg                        >  2:3322067/1‑93 (MQ=255)
                                                                cTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACg                        >  1:5893751/1‑93 (MQ=255)
                                                                 ttCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGt                       <  2:1255486/93‑1 (MQ=255)
                                                                 ttCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGt                       <  2:3799919/93‑1 (MQ=255)
                                                                  tcaatcaaTACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTg                      >  2:5854795/1‑93 (MQ=255)
                                                                  tcaatcaaTACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTg                      >  1:4172775/1‑93 (MQ=255)
                                                                  tcaatcaaTACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTg                      >  2:1290737/1‑93 (MQ=255)
                                                                    aatcaatACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTgca                    >  1:4708409/1‑91 (MQ=255)
                                                                    aatcaatACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGaa                    <  1:2172731/93‑1 (MQ=255)
                                                                      tcaatACCGCGAAATAATGCTGGAAACAGCGATCGAAAATTGTTCCTGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGCTGTGAAtt                  >  1:4586668/1‑93 (MQ=255)
                                                                       caatACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTa                 <  2:1951398/93‑1 (MQ=255)
                                                                        aatACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTAt                >  2:4327056/1‑93 (MQ=255)
                                                                         atACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATg               >  1:2848767/1‑93 (MQ=255)
                                                                          tACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATgg              <  2:3548908/93‑1 (MQ=255)
                                                                           aCCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGt             <  2:5585721/93‑1 (MQ=255)
                                                                            ccGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGtt            <  2:2473496/93‑1 (MQ=255)
                                                                             cgcgAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGttt           <  1:2049890/93‑1 (MQ=255)
                                                                                gAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACt        >  1:5526898/1‑93 (MQ=255)
                                                                                 aaaTAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTg       >  2:4945635/1‑93 (MQ=255)
                                                                                 aaaTAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTg       >  2:2876658/1‑93 (MQ=255)
                                                                                  aataatGCGGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTGa      >  2:6136245/1‑93 (MQ=255)
                                                                                   ataatGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTGaa     <  2:2776703/93‑1 (MQ=255)
                                                                                   ataatGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTGaa     <  1:1411756/93‑1 (MQ=255)
                                                                                    taatGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTGAAg    >  2:2238405/1‑93 (MQ=255)
                                                                                     aatGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTGAAgg   <  1:250805/93‑1 (MQ=255)
                                                                                     aatGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTGAAgg   <  1:4369218/93‑1 (MQ=255)
                                                                                      atGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTGAAGGc  <  1:6023846/93‑1 (MQ=255)
                                                                                      atGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTGAAGGc  <  1:5296912/93‑1 (MQ=255)
                                                                                          |                                                                                        
GGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCCGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTGAAGGC  >  NC_000913/1288670‑1288848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCCGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTGAAGGCGATCT  >  NC_000913/1288669‑1288853
                                                                                           |                                                                                             
TGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGG                                                                                             >  SRR4302533.2254603/1‑94 (MQ=60)
TGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGG                                                                                             <  SRR4302533.3176297/94‑1 (MQ=60)
   GATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAA                                                                                          >  SRR4302533.166818/1‑94 (MQ=60)
   GATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAA                                                                                          >  SRR4302533.1405312/1‑94 (MQ=60)
    ATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAAC                                                                                         >  SRR4302533.1422927/1‑94 (MQ=60)
    ATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAAC                                                                                         <  SRR4302533.2833692/94‑1 (MQ=60)
     TTTTTTCCGCCTGATGGATCTCTCCTGGCAGCGCGGTGTGTTACTGCTCTGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACA                                                                                        <  SRR4302533.740126/94‑1 (MQ=60)
      GTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAG                                                                                       >  SRR4302533.459269/1‑94 (MQ=60)
        TTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAG                                                                                     <  SRR4302533.568687/94‑1 (MQ=60)
        TTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAG                                                                                     <  SRR4302533.5080614/94‑1 (MQ=60)
         TTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGA                                                                                    <  SRR4302533.113062/94‑1 (MQ=60)
          TACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGAT                                                                                   <  SRR4302533.1336739/94‑1 (MQ=60)
           ACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATC                                                                                  >  SRR4302533.5701254/1‑94 (MQ=60)
            CGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCG                                                                                 >  SRR4302533.81276/1‑94 (MQ=60)
            CGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCG                                                                                 <  SRR4302533.1099799/94‑1 (MQ=60)
               CTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAA                                                                              >  SRR4302533.3817549/1‑94 (MQ=60)
               CTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAA                                                                              <  SRR4302533.2713921/94‑1 (MQ=60)
               CTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAA                                                                              >  SRR4302533.244739/1‑94 (MQ=60)
                    GGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGT                                                                         >  SRR4302533.432124/1‑94 (MQ=60)
                       TCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCC                                                                      >  SRR4302533.1131208/1‑94 (MQ=60)
                       TCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCC                                                                      <  SRR4302533.3538639/94‑1 (MQ=60)
                       TCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCC                                                                      >  SRR4302533.3149130/1‑94 (MQ=60)
                        CTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCC                                                                     <  SRR4302533.4194954/94‑1 (MQ=60)
                         TCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCG                                                                    <  SRR4302533.5885525/94‑1 (MQ=60)
                         TCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCG                                                                    >  SRR4302533.440644/1‑94 (MQ=60)
                         TCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCG                                                                    <  SRR4302533.1061559/94‑1 (MQ=60)
                         TCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCG                                                                    <  SRR4302533.4073883/94‑1 (MQ=60)
                         TCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCG                                                                    <  SRR4302533.5306817/94‑1 (MQ=60)
                         TCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCG                                                                    <  SRR4302533.2157929/94‑1 (MQ=60)
                          CTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGT                                                                   >  SRR4302533.4855012/1‑94 (MQ=60)
                          CTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGT                                                                   >  SRR4302533.2862194/1‑94 (MQ=60)
                          CTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGC                                                                   >  SRR4302533.6256550/1‑94 (MQ=60)
                           TCCTGGCAGCGCGGTGGGGTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTC                                                                  >  SRR4302533.636542/1‑94 (MQ=60)
                                GCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTT                                                             >  SRR4302533.260010/1‑94 (MQ=60)
                                 CAGCGCGGTGGGTTACTGCGCGTCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTG                                                            <  SRR4302533.681044/94‑1 (MQ=60)
                                 CAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTG                                                            >  SRR4302533.2569653/1‑94 (MQ=60)
                                  AGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGC                                                           >  SRR4302533.1784383/1‑94 (MQ=60)
                                   GCGGGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCG                                                          <  SRR4302533.696853/94‑1 (MQ=60)
                                   GCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCG                                                          <  SRR4302533.5252712/94‑1 (MQ=60)
                                   GCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAACAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCG                                                          <  SRR4302533.3403054/94‑1 (MQ=60)
                                      ggggGGGTTGCTGCGCGGCGGGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCT                                                       <  SRR4302533.4300420/90‑1 (MQ=60)
                                      CGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCT                                                       <  SRR4302533.85183/94‑1 (MQ=60)
                                      CGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCT                                                       >  SRR4302533.1581876/1‑94 (MQ=60)
                                         TGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTT                                                    <  SRR4302533.4807981/94‑1 (MQ=60)
                                          GGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTG                                                   <  SRR4302533.3464105/94‑1 (MQ=60)
                                          GGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTG                                                   <  SRR4302533.2843909/94‑1 (MQ=60)
                                           GGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGC                                                  >  SRR4302533.664751/1‑94 (MQ=60)
                                              TACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCAC                                               <  SRR4302533.949387/94‑1 (MQ=60)
                                              TACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCAC                                               >  SRR4302533.4530541/1‑94 (MQ=60)
                                                CTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCA                                             >  SRR4302533.5917167/1‑94 (MQ=60)
                                                CTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCA                                             >  SRR4302533.2396639/1‑94 (MQ=60)
                                                  GCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAAT                                           <  SRR4302533.2288899/94‑1 (MQ=60)
                                                   CGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATT                                          <  SRR4302533.2901392/94‑1 (MQ=60)
                                                     CGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTA                                        >  SRR4302533.1887365/1‑94 (MQ=60)
                                                     CGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTA                                        >  SRR4302533.5320738/1‑94 (MQ=60)
                                                     CGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTA                                        >  SRR4302533.6217236/1‑94 (MQ=60)
                                                       GCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAG                                      <  SRR4302533.3924020/94‑1 (MQ=60)
                                                        GGAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGT                                     <  SRR4302533.4495353/94‑1 (MQ=60)
                                                         GAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGACAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTA                                    <  SRR4302533.4473278/94‑1 (MQ=60)
                                                         GAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTA                                    >  SRR4302533.5321362/1‑94 (MQ=60)
                                                         GAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTA                                    <  SRR4302533.1190723/94‑1 (MQ=60)
                                                         GAGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTA                                    >  SRR4302533.3786600/1‑94 (MQ=60)
                                                          AGCGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTAC                                   >  SRR4302533.69047/1‑94 (MQ=60)
                                                            CGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGG                                 >  SRR4302533.217609/1‑94 (MQ=60)
                                                            CGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGG                                 <  SRR4302533.2883170/94‑1 (MQ=60)
                                                            CGTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGG                                 <  SRR4302533.5101883/94‑1 (MQ=60)
                                                             GTGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGG                                >  SRR4302533.578375/1‑94 (MQ=60)
                                                              TGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGA                               <  SRR4302533.2604819/94‑1 (MQ=60)
                                                              TGTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGA                               >  SRR4302533.112308/1‑94 (MQ=60)
                                                               GTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGAC                              >  SRR4302533.1775114/1‑94 (MQ=60)
                                                               GTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGAC                              <  SRR4302533.2419058/94‑1 (MQ=60)
                                                               GTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGAC                              >  SRR4302533.928453/1‑94 (MQ=60)
                                                               GTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGAC                              >  SRR4302533.853602/1‑94 (MQ=60)
                                                               GTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGAC                              >  SRR4302533.3416371/1‑94 (MQ=60)
                                                               GTCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGAC                              >  SRR4302533.79074/1‑94 (MQ=60)
                                                                TCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACG                             <  SRR4302533.991844/94‑1 (MQ=60)
                                                                TCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACG                             <  SRR4302533.215600/94‑1 (MQ=60)
                                                                TCTTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACG                             >  SRR4302533.5929349/1‑94 (MQ=60)
                                                                  TTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTG                           >  SRR4302533.1952672/1‑94 (MQ=60)
                                                                  TTCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTG                           >  SRR4302533.4198062/1‑94 (MQ=60)
                                                                   TCAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGA                          >  SRR4302533.5249080/1‑94 (MQ=60)
                                                                    CAATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGCA                         >  SRR4302533.4736946/1‑94 (MQ=60)
                                                                     AATCAATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAAT                        <  SRR4302533.2186091/94‑1 (MQ=60)
                                                                      ATCAATACCGCGAAATAATGCTGGAAACAGCGATCGAAAATTGTTCCTGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGCTGTGAATT                       >  SRR4302533.4614453/1‑94 (MQ=60)
                                                                        CAATACCGCGGAATAATGCTGGAAACTGCGATCGAAATTTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTAT                     <  SRR4302533.6336049/94‑1 (MQ=60)
                                                                         AATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATG                    >  SRR4302533.2866294/1‑94 (MQ=60)
                                                                         AATACCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATG                    >  SRR4302533.2880475/1‑94 (MQ=60)
                                                                             CCGCGAAATAATGCTGGAATCAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTT                <  SRR4302533.5440750/94‑1 (MQ=60)
                                                                             CCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTT                <  SRR4302533.2491978/94‑1 (MQ=60)
                                                                             CCGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTT                <  SRR4302533.5120782/94‑1 (MQ=60)
                                                                              CGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTA               <  SRR4302533.4765549/94‑1 (MQ=60)
                                                                              CGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTA               <  SRR4302533.2062536/94‑1 (MQ=60)
                                                                              CGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTA               >  SRR4302533.3697416/1‑94 (MQ=60)
                                                                              CGCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTA               >  SRR4302533.5753/1‑94 (MQ=60)
                                                                               GCGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTAC              <  SRR4302533.5083561/94‑1 (MQ=60)
                                                                                CGAAATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACT             >  SRR4302533.5560263/1‑94 (MQ=60)
                                                                                    ATAATGCTGGAAACAGAGATCGAAAGTTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTGAAG         <  SRR4302533.4327117/94‑1 (MQ=60)
                                                                                    ATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTGAAG         <  SRR4302533.4713607/94‑1 (MQ=60)
                                                                                    ATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTGAAG         >  SRR4302533.585898/1‑94 (MQ=60)
                                                                                    ATAATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTGAAG         <  SRR4302533.1420291/94‑1 (MQ=60)
                                                                                      AATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTGAAGGC       <  SRR4302533.4395591/94‑1 (MQ=60)
                                                                                      AATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTGAAGGC       <  SRR4302533.252351/94‑1 (MQ=60)
                                                                                       ATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTGAAGGCG      <  SRR4302533.6060351/94‑1 (MQ=60)
                                                                                       ATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTGAAGGCG      <  SRR4302533.6060350/94‑1 (MQ=60)
                                                                                       ATGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTGAAGGCG      <  SRR4302533.5328636/94‑1 (MQ=60)
                                                                                        TGCTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTGAAGGAGA     >  SRR4302533.4424420/1‑94 (MQ=60)
                                                                                          CTGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTGAAGGCGATC   >  SRR4302533.2627309/1‑94 (MQ=60)
                                                                                           TGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTGAAGGCGATCT  <  SRR4302533.5698579/94‑1 (MQ=60)
                                                                                           |                                                                                             
TGGGATGTTTTACGCCTGATGGATCTCTCCTGGCAGCGCGGTGGGTTACTGCGCGGCGAGCGTGTCTTCAATCAATACCGCGAAATAATGCCGGAAACAGAGATCGAAAATTGTTCCCGTCGCTTTGCGGCTGTTGCCACCAATTTAAGTACGGGACGTGAATTATGGTTTACTGAAGGCGATCT  >  NC_000913/1288669‑1288853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑