Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,291,867 A→G R93R (CGT→CGC)  hns ← DNA‑binding protein HLP‑II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,291,8670AG100.0% 584.3 / NA 182R93R (CGT→CGC) hnsDNA‑binding protein HLP‑II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (92/90);  total (92/90)

Only 100 of 189 total aligned reads displayed.

ATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCAT  >  NC_000913/1291778‑1291956
                                                                                         |                                                                                         
aTTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGgcgc                                                                                        >  2:3084225/1‑93 (MQ=255)
 ttGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCt                                                                                       >  1:1092566/1‑93 (MQ=255)
   gCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCGACGTAGCTATATTTTGCCGGGGGCTGa                                                                                     >  2:3608960/1‑93 (MQ=255)
   gCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGa                                                                                     <  1:3566578/93‑1 (MQ=255)
    cTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAg                                                                                    <  1:3780581/93‑1 (MQ=255)
      tttttGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCa                                                                                  >  1:6274722/1‑93 (MQ=255)
      tttttGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCa                                                                                  <  2:4737602/93‑1 (MQ=255)
      tttttGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCa                                                                                  <  1:4414843/93‑1 (MQ=255)
         ttGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGt                                                                               >  1:4104538/1‑93 (MQ=255)
            aTTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTa                                                                            <  2:5518094/93‑1 (MQ=255)
               aCAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCt                                                                         >  1:4333637/1‑93 (MQ=255)
                 aGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCttt                                                                       <  2:4315865/93‑1 (MQ=255)
                 aGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCttt                                                                       >  1:6115985/1‑93 (MQ=255)
                 aGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCttt                                                                       <  1:1873877/93‑1 (MQ=255)
                  gCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTg                                                                      >  1:1408558/1‑93 (MQ=255)
                  gCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTg                                                                      >  1:2387514/1‑93 (MQ=255)
                  gCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTg                                                                      <  2:5423937/93‑1 (MQ=255)
                  gCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTg                                                                      >  2:4520651/1‑93 (MQ=255)
                   cTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTgg                                                                     <  1:1617694/93‑1 (MQ=255)
                    tGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGt                                                                    <  1:1122843/93‑1 (MQ=255)
                    tGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGt                                                                    >  2:370699/1‑93 (MQ=255)
                     ggAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTg                                                                   <  2:3001619/93‑1 (MQ=255)
                     ggAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTg                                                                   >  1:119193/1‑93 (MQ=255)
                      gAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGc                                                                  >  2:177304/1‑93 (MQ=255)
                        gTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCa                                                                <  1:5544331/93‑1 (MQ=255)
                         tACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAg                                                               >  2:3516137/1‑93 (MQ=255)
                           cGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGAt                                                             <  2:2465123/93‑1 (MQ=255)
                           cGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGAt                                                             >  2:652254/1‑93 (MQ=255)
                             gCCTTGGCCAGTCGAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGAttt                                                           >  2:6194848/1‑93 (MQ=255)
                             gCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGAttt                                                           >  2:763945/1‑93 (MQ=255)
                              ccTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTa                                                          >  2:1085735/1‑93 (MQ=255)
                              ccTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTa                                                          <  1:3139036/93‑1 (MQ=255)
                                ttGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAAc                                                        <  2:4921919/93‑1 (MQ=255)
                                 tGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACg                                                       <  1:2877810/93‑1 (MQ=255)
                                  ggCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACgg                                                      <  2:1427268/93‑1 (MQ=255)
                                  ggCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACgg                                                      <  1:4565690/93‑1 (MQ=255)
                                      aGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGgcagc                                                  >  2:1258735/1‑93 (MQ=255)
                                      aGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGgcagc                                                  <  1:2850234/93‑1 (MQ=255)
                                       gTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGgcagca                                                 <  2:3817992/93‑1 (MQ=255)
                                        tCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCaa                                                >  1:2200325/1‑93 (MQ=255)
                                        tCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCaa                                                <  1:3487127/93‑1 (MQ=255)
                                         ccAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAg                                               >  2:2272569/1‑93 (MQ=255)
                                          cAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAgg                                              <  1:5216344/93‑1 (MQ=255)
                                          cAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAgg                                              >  1:3345240/1‑93 (MQ=255)
                                          cAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAgg                                              <  1:2766652/93‑1 (MQ=255)
                                          cAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAgg                                              >  1:3647380/1‑93 (MQ=255)
                                           aGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGc                                             <  1:4277039/93‑1 (MQ=255)
                                            ggTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCt                                            <  2:5554879/93‑1 (MQ=255)
                                             gTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTa                                           >  2:5952157/1‑93 (MQ=255)
                                              ttttAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTAt                                          <  2:2915115/93‑1 (MQ=255)
                                              ttttAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTAt                                          >  2:3393969/1‑93 (MQ=255)
                                               tttAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTAtt                                         <  1:6261410/93‑1 (MQ=255)
                                               tttAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTAtt                                         >  1:5056668/1‑93 (MQ=255)
                                                ttAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTc                                        >  1:2078617/1‑93 (MQ=255)
                                                ttAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTc                                        <  2:2476406/93‑1 (MQ=255)
                                                ttAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTc                                        >  1:3986548/1‑93 (MQ=255)
                                                 tAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTca                                       >  1:1049346/1‑93 (MQ=255)
                                                       cGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAgtt                                 <  2:2987359/93‑1 (MQ=255)
                                                        gCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAgttc                                <  1:2520769/93‑1 (MQ=255)
                                                         ccGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAgttcg                               <  1:843072/93‑1 (MQ=255)
                                                          cGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAgttcgt                              >  1:4799632/1‑93 (MQ=255)
                                                          cGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAgttcgt                              <  1:5487848/93‑1 (MQ=255)
                                                           gTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAgttcgtt                             <  1:3867367/93‑1 (MQ=255)
                                                           gTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAgttcgtt                             <  1:3265663/93‑1 (MQ=255)
                                                            ttttCGTCAACGTAGCTATATTTTGCGGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAgttcgttc                            >  1:3185266/1‑93 (MQ=255)
                                                            ttttCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAgttcgttc                            <  2:519384/93‑1 (MQ=255)
                                                            ttttCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAgttcgttc                            <  2:5405276/93‑1 (MQ=255)
                                                                 gTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTc                       >  2:2833738/1‑93 (MQ=255)
                                                                  tCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGTTTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCa                      <  2:4384829/93‑1 (MQ=255)
                                                                  tCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCa                      <  2:1202049/93‑1 (MQ=255)
                                                                   cAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCaa                     <  1:2560957/93‑1 (MQ=255)
                                                                    aaCGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAAt                    >  2:197241/1‑93 (MQ=255)
                                                                    aaCGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAAt                    >  1:5359793/1‑93 (MQ=255)
                                                                     aCGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGTAAGGCTATTCAGCAGTTCGTTCGGGTCAAtt                   >  2:2737059/1‑92 (MQ=255)
                                                                     aCGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATa                   <  1:5888729/93‑1 (MQ=255)
                                                                     aCGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATa                   <  1:5273073/93‑1 (MQ=255)
                                                                       gTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATAcc                 <  2:2428667/93‑1 (MQ=255)
                                                                        tAGCTATATTTTGCCGGGCGCTGAGCTCGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCg                <  2:5091943/93‑1 (MQ=255)
                                                                        tAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCg                >  1:4697290/1‑93 (MQ=255)
                                                                        tAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGGCAATACCg                >  2:2642112/1‑93 (MQ=255)
                                                                         aGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGt               >  2:2366454/1‑93 (MQ=255)
                                                                         aGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGt               <  2:5728277/93‑1 (MQ=255)
                                                                          gCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTc              >  1:5647431/1‑93 (MQ=255)
                                                                           cTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCa             <  1:911178/93‑1 (MQ=255)
                                                                             atatTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGc           >  2:1637444/1‑93 (MQ=255)
                                                                             atatTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGc           >  1:691193/1‑93 (MQ=255)
                                                                             atatTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGc           >  2:834602/1‑93 (MQ=255)
                                                                              tatTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCg          >  2:3178153/1‑93 (MQ=255)
                                                                              tatTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCg          >  2:2231291/1‑93 (MQ=255)
                                                                              tatTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCg          >  1:887034/1‑93 (MQ=255)
                                                                              tatTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCg          >  1:325449/1‑93 (MQ=255)
                                                                               atTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGa         >  1:2147012/1‑93 (MQ=255)
                                                                               atTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGa         <  1:3019356/93‑1 (MQ=255)
                                                                                ttttGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCCGCGAt        >  2:4498419/1‑93 (MQ=255)
                                                                                  ttGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCa      <  1:3942336/93‑1 (MQ=255)
                                                                                  ttGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCa      <  1:3115843/93‑1 (MQ=255)
                                                                                    gCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGc    >  2:400066/1‑93 (MQ=255)
                                                                                     ccGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCa   <  1:4938498/93‑1 (MQ=255)
                                                                                     ccGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCa   <  1:2256583/93‑1 (MQ=255)
                                                                                      cGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCAt  >  1:382343/1‑93 (MQ=255)
                                                                                         |                                                                                         
ATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCAT  >  NC_000913/1291778‑1291956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCATTT  >  NC_000913/1291774‑1291958
                                                                                             |                                                                                           
ATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGG                                                                                             >  SRR4302533.6093618/1‑94 (MQ=60)
ATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGG                                                                                             <  SRR4302533.3655593/94‑1 (MQ=60)
 TCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGC                                                                                            >  SRR4302533.3472523/1‑94 (MQ=60)
 TCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGC                                                                                            >  SRR4302533.5648601/1‑94 (MQ=60)
   CATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGGCGGGCGC                                                                                          >  SRR4302533.285742/1‑94 (MQ=60)
   CATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGC                                                                                          >  SRR4302533.3890704/1‑94 (MQ=60)
    ATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCT                                                                                         >  SRR4302533.1099028/1‑94 (MQ=60)
    ATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCT                                                                                         >  SRR4302533.2995301/1‑94 (MQ=60)
    ATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCT                                                                                         <  SRR4302533.5836112/94‑1 (MQ=60)
       GCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAG                                                                                      <  SRR4302533.3588557/94‑1 (MQ=60)
        CTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGC                                                                                     >  SRR4302533.2502108/1‑94 (MQ=60)
        CTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGC                                                                                     <  SRR4302533.3803741/94‑1 (MQ=60)
         TTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCA                                                                                    <  SRR4302533.1570402/94‑1 (MQ=60)
         TTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCA                                                                                    >  SRR4302533.6312196/1‑94 (MQ=60)
         TTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCA                                                                                    >  SRR4302533.3205836/1‑94 (MQ=60)
          TTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCAC                                                                                   <  SRR4302533.4441502/94‑1 (MQ=60)
            TTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGT                                                                                 <  SRR4302533.4278761/94‑1 (MQ=60)
            TTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGT                                                                                 >  SRR4302533.4129503/1‑94 (MQ=60)
             TTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTT                                                                                <  SRR4302533.5314699/94‑1 (MQ=60)
               GATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTA                                                                              >  SRR4302533.6303721/1‑94 (MQ=60)
                  TACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCT                                                                           >  SRR4302533.2134609/1‑94 (MQ=60)
                  TACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCT                                                                           >  SRR4302533.4359749/1‑94 (MQ=60)
                    CAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTT                                                                         >  SRR4302533.6152883/1‑94 (MQ=60)
                     AGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTG                                                                        >  SRR4302533.1417072/1‑94 (MQ=60)
                     AGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTG                                                                        >  SRR4302533.2402264/1‑94 (MQ=60)
                     AGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTG                                                                        <  SRR4302533.1885296/94‑1 (MQ=60)
                       CTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGT                                                                      <  SRR4302533.1627495/94‑1 (MQ=60)
                       CTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGCTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGG                                                                      >  SRR4302533.3432288/1‑94 (MQ=60)
                        TGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTG                                                                     <  SRR4302533.1129508/94‑1 (MQ=60)
                        TGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTG                                                                     >  SRR4302533.119949/1‑94 (MQ=60)
                           AGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCA                                                                  <  SRR4302533.3342277/94‑1 (MQ=60)
                            GTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAG                                                                 <  SRR4302533.5577815/94‑1 (MQ=60)
                              ACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGAT                                                               <  SRR4302533.5464813/94‑1 (MQ=60)
                                GGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTT                                                             >  SRR4302533.520559/1‑94 (MQ=60)
                                GGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTT                                                             >  SRR4302533.3284867/1‑94 (MQ=60)
                                 GCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTA                                                            >  SRR4302533.1841730/1‑94 (MQ=60)
                                  CCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAA                                                           >  SRR4302533.371667/1‑94 (MQ=60)
                                  CCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAA                                                           <  SRR4302533.3158333/94‑1 (MQ=60)
                                   CTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAAC                                                          <  SRR4302533.2368832/94‑1 (MQ=60)
                                    TTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACG                                                         <  SRR4302533.2005757/94‑1 (MQ=60)
                                    TTGGCCAGTCCAGCTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACG                                                         <  SRR4302533.3625682/94‑1 (MQ=60)
                                     TGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGG                                                        <  SRR4302533.2895519/94‑1 (MQ=60)
                                     TGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGG                                                        <  SRR4302533.652586/94‑1 (MQ=60)
                                      GGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGC                                                       >  SRR4302533.4729254/1‑94 (MQ=60)
                                      GGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGC                                                       <  SRR4302533.4593370/94‑1 (MQ=60)
                                        CCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAG                                                     >  SRR4302533.3991973/1‑94 (MQ=60)
                                         CAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGC                                                    >  SRR4302533.4869615/1‑94 (MQ=60)
                                          AGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCA                                                   <  SRR4302533.2867770/94‑1 (MQ=60)
                                          AGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCA                                                   >  SRR4302533.2870859/1‑94 (MQ=60)
                                           GTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAA                                                  >  SRR4302533.2213880/1‑94 (MQ=60)
                                            TCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAG                                                 <  SRR4302533.3508629/94‑1 (MQ=60)
                                             CCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGG                                                >  SRR4302533.3669805/1‑94 (MQ=60)
                                             CCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGG                                                >  SRR4302533.3365908/1‑94 (MQ=60)
                                              CAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGC                                               <  SRR4302533.5247750/94‑1 (MQ=60)
                                              CAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGC                                               <  SRR4302533.2783624/94‑1 (MQ=60)
                                               AGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCT                                              <  SRR4302533.4302761/94‑1 (MQ=60)
                                               AGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCT                                              >  SRR4302533.908518/1‑94 (MQ=60)
                                                 GTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTAT                                            >  SRR4302533.4552555/1‑94 (MQ=60)
                                                  TTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATT                                           >  SRR4302533.5087232/1‑94 (MQ=60)
                                                   TTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTC                                          >  SRR4302533.4010829/1‑94 (MQ=60)
                                                   TTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTC                                          >  SRR4302533.2091409/1‑94 (MQ=60)
                                                   TTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTC                                          >  SRR4302533.2537865/1‑94 (MQ=60)
                                                   TTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTC                                          <  SRR4302533.6298844/94‑1 (MQ=60)
                                                    TTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCA                                         >  SRR4302533.1055514/1‑94 (MQ=60)
                                                    TTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCA                                         <  SRR4302533.6021679/94‑1 (MQ=60)
                                                          TCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTT                                   >  SRR4302533.4306387/1‑94 (MQ=60)
                                                           CGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTC                                  >  SRR4302533.3078009/1‑94 (MQ=60)
                                                           CGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTC                                  <  SRR4302533.624396/94‑1 (MQ=60)
                                                           CGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTC                                  <  SRR4302533.2195153/94‑1 (MQ=60)
                                                            GCCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCG                                 <  SRR4302533.2536302/94‑1 (MQ=60)
                                                             CCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGT                                <  SRR4302533.848003/94‑1 (MQ=60)
                                                             CCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGT                                >  SRR4302533.3188023/1‑94 (MQ=60)
                                                             CCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGT                                >  SRR4302533.4828705/1‑94 (MQ=60)
                                                             CCGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGT                                >  SRR4302533.871136/1‑94 (MQ=60)
                                                              CGTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTT                               <  SRR4302533.5520931/94‑1 (MQ=60)
                                                               GTTTTCGTCAACGTAGCTATATTTTGCGGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTC                              >  SRR4302533.3204856/1‑94 (MQ=60)
                                                               GTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTGACGGCAGCAAGGCTATTCAGCAGTTCGTTC                              >  SRR4302533.3512351/1‑94 (MQ=60)
                                                               GTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTC                              <  SRR4302533.3890973/94‑1 (MQ=60)
                                                               GTTTTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTC                              <  SRR4302533.3285815/94‑1 (MQ=60)
                                                                  TTCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGG                           <  SRR4302533.1295350/94‑1 (MQ=60)
                                                                   TCGTCAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGT                          <  SRR4302533.5259536/94‑1 (MQ=60)
                                                                       CAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAAT                      <  SRR4302533.2576728/94‑1 (MQ=60)
                                                                       CAACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAAT                      >  SRR4302533.5391978/1‑94 (MQ=60)
                                                                        AACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATA                     <  SRR4302533.5558385/94‑1 (MQ=60)
                                                                        AACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATA                     <  SRR4302533.5012497/94‑1 (MQ=60)
                                                                         ACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATAC                    <  SRR4302533.2699044/94‑1 (MQ=60)
                                                                         ACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATAC                    <  SRR4302533.5304692/94‑1 (MQ=60)
                                                                         ACGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATAC                    <  SRR4302533.5924306/94‑1 (MQ=60)
                                                                          CGTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACC                   >  SRR4302533.4369408/1‑94 (MQ=60)
                                                                           GTAGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCG                  >  SRR4302533.4725767/1‑94 (MQ=60)
                                                                             AGCTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTC                >  SRR4302533.5681570/1‑94 (MQ=60)
                                                                               CTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAG              <  SRR4302533.916521/94‑1 (MQ=60)
                                                                               CTATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAG              >  SRR4302533.4026099/1‑94 (MQ=60)
                                                                                TATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGC             <  SRR4302533.1172642/94‑1 (MQ=60)
                                                                                TATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGC             >  SRR4302533.695279/1‑94 (MQ=60)
                                                                                 ATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCG            >  SRR4302533.892241/1‑94 (MQ=60)
                                                                                 ATATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCG            >  SRR4302533.327435/1‑94 (MQ=60)
                                                                                  TATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGA           >  SRR4302533.2160235/1‑94 (MQ=60)
                                                                                   ATTTTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGAT          <  SRR4302533.3037838/94‑1 (MQ=60)
                                                                                    TTTTTCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATC         >  SRR4302533.3940956/1‑94 (MQ=60)
                                                                                      TTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAG       <  SRR4302533.3966390/94‑1 (MQ=60)
                                                                                      TTGCCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAG       <  SRR4302533.3135000/94‑1 (MQ=60)
                                                                                         CCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCAT    <  SRR4302533.1128891/94‑1 (MQ=60)
                                                                                         CCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCAT    <  SRR4302533.4968394/94‑1 (MQ=60)
                                                                                         CCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCAT    <  SRR4302533.2270546/94‑1 (MQ=60)
                                                                                         CCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCAT    <  SRR4302533.723423/94‑1 (MQ=60)
                                                                                         CCGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCAT    >  SRR4302533.384648/1‑94 (MQ=60)
                                                                                          CGGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCATT   >  SRR4302533.3457460/1‑94 (MQ=60)
                                                                                           GGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCATTT  <  SRR4302533.4527110/94‑1 (MQ=60)
                                                                                           GGGCGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCATTT  >  SRR4302533.4648458/1‑94 (MQ=60)
                                                                                             |                                                                                           
ATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCATTT  >  NC_000913/1291774‑1291958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑