Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,308,235	T→C	H20R (CAC→CGC)	kch ←	putative potassium channel protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,308,235	0	T	C	99.5%	684.5 / ‑5.8	214	H20R (CAC→CGC)	kch	putative potassium channel protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (139/74); total (139/75)
Fisher's exact test for biased strand distribution p-value = 3.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 225 total aligned reads displayed.

TGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGTTTAAT  >  NC_000913/1308145‑1308324

tGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCgcgc                                                                                         >  2:2246222/1‑93 (MQ=255)

 ggAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGcc                                                                                        >  1:4893615/1‑93 (MQ=255)

 ggAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGcc                                                                                        <  2:4880012/93‑1 (MQ=255)

  gAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCg                                                                                       >  1:5047621/1‑93 (MQ=255)

    aaaTATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGaa                                                                                     <  1:3452630/93‑1 (MQ=255)

     aaTATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAg                                                                                    >  2:5949274/1‑93 (MQ=255)

     aaTATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAg                                                                                    >  1:4645822/1‑93 (MQ=255)

         tCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTc                                                                                >  1:1206897/1‑93 (MQ=255)

         tCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTc                                                                                <  2:3703311/93‑1 (MQ=255)

         tCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTc                                                                                >  2:3360698/1‑93 (MQ=255)

          cAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCa                                                                               >  1:5541529/1‑93 (MQ=255)

          cAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCa                                                                               <  1:2132017/93‑1 (MQ=255)

           aaGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCAc                                                                              >  1:1855286/1‑93 (MQ=255)

            aGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCAcc                                                                             <  2:5060706/93‑1 (MQ=255)

             gCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCAccc                                                                            <  1:5665032/93‑1 (MQ=255)

              cAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCa                                                                           <  1:2756003/93‑1 (MQ=255)

               aaaTTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCAt                                                                          >  2:1864938/1‑93 (MQ=255)

               aaaTTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCAt                                                                          <  1:4499272/93‑1 (MQ=255)

                aaTTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATa                                                                         >  2:1727668/1‑93 (MQ=255)

                aaTTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATa                                                                         >  1:3282152/1‑93 (MQ=255)

                 aTTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATaa                                                                        >  2:2428167/1‑93 (MQ=255)

                  ttGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATaaa                                                                       >  1:3849450/1‑93 (MQ=255)

                    gACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAAtt                                                                     >  2:1578057/1‑93 (MQ=255)

                      ccGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATttg                                                                   <  1:667442/93‑1 (MQ=255)

                       cGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATttgt                                                                  <  2:3211110/93‑1 (MQ=255)

                         aCATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATttgttg                                                                >  1:6157035/1‑93 (MQ=255)

                           aTACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCt                                                              <  1:6039117/93‑1 (MQ=255)

                             aCCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCtgt                                                            <  2:2980644/93‑1 (MQ=255)

                              ccATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCtgtt                                                           >  2:4194545/1‑93 (MQ=255)

                               cATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCtgttt                                                          >  2:5670131/1‑93 (MQ=255)

                               cATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCtgttt                                                          <  1:5847922/93‑1 (MQ=255)

                                aTAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCtgtttg                                                         >  2:1070569/1‑93 (MQ=255)

                                  aGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCtgtttgtt                                                       >  1:5595074/1‑93 (MQ=255)

                                  aGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCtgtttgtt                                                       <  1:1606989/93‑1 (MQ=255)

                                  aGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCtgtttgtt                                                       >  1:2915600/1‑93 (MQ=255)

                                   gATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTgtttgttt                                                      >  2:1814365/1‑93 (MQ=255)

                                    aTTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGtttgtttg                                                     >  2:2718498/1‑93 (MQ=255)

                                     ttGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGa                                                    <  2:2913073/93‑1 (MQ=255)

                                     ttGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGa                                                    >  1:4812270/1‑93 (MQ=255)

                                     ttGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGa                                                    >  2:2330541/1‑93 (MQ=255)

                                      tGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGaa                                                   <  1:1356837/93‑1 (MQ=255)

                                       gTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAAt                                                  <  2:3365767/93‑1 (MQ=255)

                                       gTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAAt                                                  <  2:2652085/93‑1 (MQ=255)

                                       gTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAAt                                                  >  2:2683917/1‑93 (MQ=255)

                                        ttttAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATg                                                 <  1:4111360/93‑1 (MQ=255)

                                        ttttAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATg                                                 <  2:1495036/93‑1 (MQ=255)

                                        ttttAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATg                                                 <  2:3116403/93‑1 (MQ=255)

                                         tttAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGt                                                <  2:4766802/93‑1 (MQ=255)

                                          ttAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTa                                               <  2:1887594/93‑1 (MQ=255)

                                          ttAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTa                                               <  1:4552807/93‑1 (MQ=255)

                                           tAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAg                                              <  1:1736501/93‑1 (MQ=255)

                                           tAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAg                                              >  1:2921774/1‑93 (MQ=255)

                                            aaaaaTAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGc                                             <  2:1921009/93‑1 (MQ=255)

                                            aaaaaTAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGc                                             >  1:4724160/1‑93 (MQ=255)

                                              aaaTAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGccc                                           <  1:2677370/93‑1 (MQ=255)

                                                aTAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAg                                         <  2:5879578/93‑1 (MQ=255)

                                                 tAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGt                                        <  2:926144/93‑1 (MQ=255)

                                                 tAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGt                                        >  1:1064676/1‑93 (MQ=255)

                                                  aaGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTg                                       >  2:1131404/1‑93 (MQ=255)

                                                    gCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGAc                                     >  1:5658616/1‑93 (MQ=255)

                                                      aaTCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTc                                   >  2:2918485/1‑93 (MQ=255)

                                                       aTCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCa                                  >  1:2025274/1‑93 (MQ=255)

                                                       aTCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCa                                  >  1:264558/1‑93 (MQ=255)

                                                        tCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCAc                                 <  2:3544217/93‑1 (MQ=255)

                                                        tCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCAc                                 >  2:2501379/1‑93 (MQ=255)

                                                        tCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCAc                                 >  1:3060501/1‑93 (MQ=255)

                                                         ccATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACt                                <  2:380160/93‑1 (MQ=255)

                                                         ccATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACt                                >  1:3068700/1‑93 (MQ=255)

                                                          cATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTg                               >  2:5425072/1‑93 (MQ=255)

                                                          cATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTg                               >  1:4009486/1‑93 (MQ=255)

                                                           aTTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGa                              >  2:6285316/1‑93 (MQ=255)

                                                           aTTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGa                              <  1:1124609/93‑1 (MQ=255)

                                                            tttAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGAt                             <  1:2474853/93‑1 (MQ=255)

                                                            tttAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGAt                             >  2:2573727/1‑93 (MQ=255)

                                                             ttAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATa                            >  2:563914/1‑93 (MQ=255)

                                                              tAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATAc                           <  1:1022790/93‑1 (MQ=255)

                                                              tAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATAc                           <  1:4281864/93‑1 (MQ=255)

                                                               aaaaaGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATAcc                          >  1:3344987/1‑93 (MQ=255)

                                                                aaaaGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACct                         >  2:5291254/1‑93 (MQ=255)

                                                                aaaaGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACct                         >  1:3250216/1‑93 (MQ=255)

                                                                 aaaGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACctc                        >  2:4176997/1‑93 (MQ=255)

                                                                 aaaGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACctc                        <  1:5302217/93‑1 (MQ=255)

                                                                    gACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGt                     >  1:2396866/1‑93 (MQ=255)

                                                                     aCGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTa                    >  1:2306910/1‑93 (MQ=255)

                                                                       ggCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAAc                  >  2:3910044/1‑93 (MQ=255)

                                                                          cAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACcac               >  1:950384/1‑93 (MQ=255)

                                                                           aGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACcaca              >  2:1614313/1‑93 (MQ=255)

                                                                            gcgcGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACaa             >  2:4387878/1‑93 (MQ=255)

                                                                             cgcgAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAAt            <  1:2728447/93‑1 (MQ=255)

                                                                              gcgAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAAtt           >  2:1990400/1‑93 (MQ=255)

                                                                               cgAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAAttt          >  1:1825361/1‑93 (MQ=255)

                                                                                gagaATGTCGTGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAAtttt         <  1:5345316/93‑1 (MQ=255)

                                                                                gagaATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAAtttt         >  2:5520779/1‑93 (MQ=255)

                                                                                 agaATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTg        >  1:3828553/1‑93 (MQ=255)

                                                                                 agaATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAACGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTg        >  1:4154969/1‑93 (MQ=255)

                                                                                  gaATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGt       >  2:4962099/1‑93 (MQ=255)

                                                                                  gaATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGt       >  2:3970058/1‑93 (MQ=255)

                                                                                   aaTGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGtt      >  1:5168123/1‑93 (MQ=255)

                                                                                    aTGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGttt     >  1:1512385/1‑93 (MQ=255)

                                                                                     tGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGTTTa    <  1:1554583/93‑1 (MQ=255)

TGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGTTTAAT  >  NC_000913/1308145‑1308324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATATGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGTTTAATTATC  >  NC_000913/1308142‑1308328

ATATGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGC                                                                                               >  SRR4302533.4529555/1‑94 (MQ=60)

ATATGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGC                                                                                               >  SRR4302533.4269574/1‑94 (MQ=60)

 TATGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCG                                                                                              <  SRR4302533.1918084/94‑1 (MQ=60)

 TATGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCG                                                                                              <  SRR4302533.1139686/94‑1 (MQ=60)

 TATGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCG                                                                                              <  SRR4302533.5220312/94‑1 (MQ=60)

  ATGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGC                                                                                             >  SRR4302533.5819563/1‑94 (MQ=60)

  ATGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGC                                                                                             <  SRR4302533.4838484/94‑1 (MQ=60)

  ATGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGC                                                                                             >  SRR4302533.2825490/1‑94 (MQ=60)

   TGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCC                                                                                            >  SRR4302533.4923275/1‑94 (MQ=60)

   TGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCC                                                                                            <  SRR4302533.1886137/94‑1 (MQ=60)

    GGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCG                                                                                           >  SRR4302533.1538081/1‑94 (MQ=60)

    GGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCG                                                                                           >  SRR4302533.5078153/1‑94 (MQ=60)

    GGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCG                                                                                           >  SRR4302533.698837/1‑94 (MQ=60)

     GAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGA                                                                                          >  SRR4302533.5592820/1‑94 (MQ=60)

       AAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAG                                                                                        <  SRR4302533.3473947/94‑1 (MQ=60)

       AAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAG                                                                                        >  SRR4302533.4673977/1‑94 (MQ=60)

          TATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGT                                                                                     >  SRR4302533.753244/1‑94 (MQ=60)

           ATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTC                                                                                    >  SRR4302533.1214144/1‑94 (MQ=60)

            TCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCA                                                                                   >  SRR4302533.5574994/1‑94 (MQ=60)

            TCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCA                                                                                   >  SRR4302533.5415757/1‑94 (MQ=60)

             CAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCAC                                                                                  <  SRR4302533.2145165/94‑1 (MQ=60)

             CAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCAC                                                                                  >  SRR4302533.5844106/1‑94 (MQ=60)

             CAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCAC                                                                                  >  SRR4302533.1866599/1‑94 (MQ=60)

             CAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCAC                                                                                  >  SRR4302533.2960740/1‑94 (MQ=60)

             CAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCAC                                                                                  >  SRR4302533.4001569/1‑94 (MQ=60)

                GCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCA                                                                               <  SRR4302533.5699260/94‑1 (MQ=60)

                 CAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCAT                                                                              <  SRR4302533.2772905/94‑1 (MQ=60)

                 CAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCAT                                                                              >  SRR4302533.982431/1‑94 (MQ=60)

                  AAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATA                                                                             <  SRR4302533.5693716/94‑1 (MQ=60)

                  AAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATA                                                                             <  SRR4302533.4526518/94‑1 (MQ=60)

                  AAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATA                                                                             >  SRR4302533.3302416/1‑94 (MQ=60)

                    ATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAA                                                                           >  SRR4302533.3872957/1‑94 (MQ=60)

                        ACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTG                                                                       >  SRR4302533.2449279/1‑94 (MQ=60)

                         CCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGT                                                                      <  SRR4302533.2662233/94‑1 (MQ=60)

                         CCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGT                                                                      <  SRR4302533.671385/94‑1 (MQ=60)

                          CGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTT                                                                     >  SRR4302533.3712277/1‑94 (MQ=60)

                          CGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTT                                                                     >  SRR4302533.487741/1‑94 (MQ=60)

                          CGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTT                                                                     >  SRR4302533.6050505/1‑94 (MQ=60)

                           GACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTG                                                                    >  SRR4302533.6194074/1‑94 (MQ=60)

                             CATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCT                                                                  >  SRR4302533.649587/1‑94 (MQ=60)

                             CATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCT                                                                  >  SRR4302533.1383107/1‑94 (MQ=60)

                              ATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTG                                                                 <  SRR4302533.6075713/94‑1 (MQ=60)

                               TACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGT                                                                <  SRR4302533.3409086/94‑1 (MQ=60)

                                ACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTT                                                               >  SRR4302533.5342078/1‑94 (MQ=60)

                                  CATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTG                                                             >  SRR4302533.3730067/1‑94 (MQ=60)

                                  CATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTG                                                             <  SRR4302533.5883273/94‑1 (MQ=60)

                                   ATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGCGACACCCATAAATTTGTTGCTGTTTGT                                                            >  SRR4302533.4688471/1‑94 (MQ=60)

                                    TAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTT                                                           >  SRR4302533.5628876/1‑94 (MQ=60)

                                    TAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTT                                                           >  SRR4302533.2933480/1‑94 (MQ=60)

                                     AGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTT                                                          <  SRR4302533.1616741/94‑1 (MQ=60)

                                       ATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGA                                                        >  SRR4302533.4841437/1‑94 (MQ=60)

                                         TGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAAT                                                      <  SRR4302533.4667871/94‑1 (MQ=60)

                                         TGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAAT                                                      <  SRR4302533.1364990/94‑1 (MQ=60)

                                         TGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAAT                                                      <  SRR4302533.272910/94‑1 (MQ=60)

                                          GTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATG                                                     <  SRR4302533.3194138/94‑1 (MQ=60)

                                           TTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGT                                                    <  SRR4302533.4136349/94‑1 (MQ=60)

                                           TTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGT                                                    <  SRR4302533.2098517/94‑1 (MQ=60)

                                            TTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTA                                                   >  SRR4302533.1220317/1‑94 (MQ=60)

                                             TTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAG                                                  <  SRR4302533.4580417/94‑1 (MQ=60)

                                             TTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAG                                                  >  SRR4302533.2939667/1‑94 (MQ=60)

                                             TTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAG                                                  >  SRR4302533.2939666/1‑94 (MQ=60)

                                              TAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGC                                                 >  SRR4302533.159526/1‑94 (MQ=60)

                                              TAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGC                                                 <  SRR4302533.1747038/94‑1 (MQ=60)

                                              TAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGC                                                 >  SRR4302533.4752804/1‑94 (MQ=60)

                                                AAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCC                                               <  SRR4302533.1042280/94‑1 (MQ=60)

                                                 AAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCA                                              <  SRR4302533.2693784/94‑1 (MQ=60)

                                                 AAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCA                                              >  SRR4302533.1099269/1‑94 (MQ=60)

                                                 AAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCA                                              <  SRR4302533.2558057/94‑1 (MQ=60)

                                                   ATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGT                                            >  SRR4302533.1070930/1‑94 (MQ=60)

                                                      AGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGAC                                         >  SRR4302533.5692808/1‑94 (MQ=60)

                                                       GCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACT                                        >  SRR4302533.3309663/1‑94 (MQ=60)

                                                        CAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTC                                       >  SRR4302533.4576639/1‑94 (MQ=60)

                                                         AATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCA                                      >  SRR4302533.266165/1‑94 (MQ=60)

                                                         AATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCA                                      >  SRR4302533.2037778/1‑94 (MQ=60)

                                                         AATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCA                                      >  SRR4302533.418089/1‑94 (MQ=60)

                                                          ATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCAC                                     >  SRR4302533.3079285/1‑94 (MQ=60)

                                                          ATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCAC                                     >  SRR4302533.3079286/1‑94 (MQ=60)

                                                          ATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCAC                                     >  SRR4302533.6095083/1‑94 (MQ=60)

                                                           TCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACT                                    >  SRR4302533.3087543/1‑94 (MQ=60)

                                                           TCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAACGGTCACCCATAAATTTGTTGCTGGTTGTTTGAATGTAGCCCAGTGACTCACT                                    >  SRR4302533.4150738/1‑94 (MQ=60)

                                                            CCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTG                                   <  SRR4302533.5680381/94‑1 (MQ=60)

                                                            CCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTG                                   >  SRR4302533.4033897/1‑94 (MQ=60)

                                                             CATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGA                                  >  SRR4302533.5235155/1‑94 (MQ=60)

                                                              ATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGAT                                 <  SRR4302533.1131284/94‑1 (MQ=60)

                                                               TTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATA                                <  SRR4302533.2490113/94‑1 (MQ=60)

                                                                 TAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACC                              <  SRR4302533.1028802/94‑1 (MQ=60)

                                                                 TAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACC                              <  SRR4302533.4990840/94‑1 (MQ=60)

                                                                 TAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACC                              >  SRR4302533.3365655/1‑94 (MQ=60)

                                                                 TAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACC                              <  SRR4302533.4307601/94‑1 (MQ=60)

                                                                  AAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCT                             >  SRR4302533.3270257/1‑94 (MQ=60)

                                                                  AAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCT                             >  SRR4302533.2549716/1‑94 (MQ=60)

                                                                    AAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCT                           <  SRR4302533.4600557/94‑1 (MQ=60)

                                                                    AAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCT                           >  SRR4302533.4055077/1‑94 (MQ=60)

                                                                    AAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCT                           <  SRR4302533.5333982/94‑1 (MQ=60)

                                                                      AGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGT                         >  SRR4302533.2411662/1‑94 (MQ=60)

                                                                       GACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTA                        <  SRR4302533.483067/94‑1 (MQ=60)

                                                                       GACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTA                        >  SRR4302533.2321201/1‑94 (MQ=60)

                                                                        ACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAA                       <  SRR4302533.3871591/94‑1 (MQ=60)

                                                                        ACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAA                       >  SRR4302533.1248086/1‑94 (MQ=60)

                                                                          GGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACC                     >  SRR4302533.1762428/1‑94 (MQ=60)

                                                                          GGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACC                     >  SRR4302533.314434/1‑94 (MQ=60)

                                                                           GCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCA                    >  SRR4302533.5406453/1‑94 (MQ=60)

                                                                            CCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCAC                   >  SRR4302533.955964/1‑94 (MQ=60)

                                                                             CAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACA                  <  SRR4302533.2199013/94‑1 (MQ=60)

                                                                             CAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACA                  >  SRR4302533.414818/1‑94 (MQ=60)

                                                                             CAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACA                  >  SRR4302533.1642876/1‑94 (MQ=60)

                                                                               GCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAAT                <  SRR4302533.1667732/94‑1 (MQ=60)

                                                                                CGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATT               <  SRR4302533.2745185/94‑1 (MQ=60)

                                                                                 GCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTT              >  SRR4302533.1836511/1‑94 (MQ=60)

                                                                                 GCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTT              >  SRR4302533.2061407/1‑94 (MQ=60)

                                                                                  CGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTT             >  SRR4302533.2765319/1‑94 (MQ=60)

                                                                                  CGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTT             >  SRR4302533.4369910/1‑94 (MQ=60)

                                                                                   GAGAATGTCGTGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTG            <  SRR4302533.5377372/94‑1 (MQ=60)

                                                                                   GAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTG            <  SRR4302533.1847767/94‑1 (MQ=60)

                                                                                   GAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTG            >  SRR4302533.5090302/1‑94 (MQ=60)

                                                                                   GAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTG            >  SRR4302533.3851962/1‑94 (MQ=60)

                                                                                   GAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGAATCACTGATACCTCTGTAACCACAATTTTG            >  SRR4302533.2794498/1‑94 (MQ=60)

                                                                                   GAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAACGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTG            >  SRR4302533.4180148/1‑94 (MQ=60)

                                                                                     GAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGTT          >  SRR4302533.5199320/1‑94 (MQ=60)

                                                                                      AATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGTTT         >  SRR4302533.235582/1‑94 (MQ=60)

                                                                                      AATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGTTT         >  SRR4302533.1521498/1‑94 (MQ=60)

                                                                                      AATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGTTT         <  SRR4302533.4990818/94‑1 (MQ=60)

                                                                                       ATGTCCCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGTTTA        <  SRR4302533.1179319/94‑1 (MQ=60)

                                                                                        TGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGTTTAA       <  SRR4302533.1563943/94‑1 (MQ=60)

                                                                                          TCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGTTTAATT     <  SRR4302533.3271718/94‑1 (MQ=60)

                                                                                            GCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATCCCTCTGTAACCACAATTTTGTTTAATTAT   <  SRR4302533.4370957/94‑1 (MQ=60)

                                                                                             CGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGTTTAATTATC  >  SRR4302533.3629193/1‑94 (MQ=60)

ATATGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGTTTAATTATC  >  NC_000913/1308142‑1308328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`