Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,308,235 T→C H20R (CAC→CGC)  kch ← putative potassium channel protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,308,2350TC90.6% 880.1 / 67.6 339H20R (CAC→CGC) kchputative potassium channel protein
Reads supporting (aligned to +/- strand):  ref base T (22/10);  new base C (181/126);  total (203/136)
Fisher's exact test for biased strand distribution p-value = 3.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 354 total aligned reads displayed.

TGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGTTTAATTAT  >  NC_000913/1308145‑1308327
                                                                                          |                                                                                            
tGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCgcgc                                                                                            >  2:7655400/1‑93 (MQ=255)
tGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCgcgc                                                                                            <  2:3630587/93‑1 (MQ=255)
 ggAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGcc                                                                                           >  1:2721824/1‑93 (MQ=255)
  gAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCg                                                                                          >  1:8279119/1‑93 (MQ=255)
   aaaaTATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGa                                                                                         >  1:8919786/1‑93 (MQ=255)
   aaaaTATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGa                                                                                         <  2:5854554/93‑1 (MQ=255)
    aaaTATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGGATGTCGCGCCGaa                                                                                        >  2:1291711/1‑93 (MQ=255)
    aaaTATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGaa                                                                                        >  2:2542030/1‑93 (MQ=255)
       tatCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGCCCAGCGCGAGAATGTCGTGCCGAAggg                                                                                     <  1:5817980/93‑1 (MQ=255)
        atCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGt                                                                                    <  2:8115182/93‑1 (MQ=255)
        atCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGt                                                                                    <  2:8255699/93‑1 (MQ=255)
         tCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTc                                                                                   <  2:3840476/93‑1 (MQ=255)
         tCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTc                                                                                   >  2:3337260/1‑93 (MQ=255)
          cAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCa                                                                                  >  2:1489792/1‑93 (MQ=255)
            aGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGGGAGAATGTCGCGCCGAAGGGTCAcc                                                                                <  1:4883723/93‑1 (MQ=255)
             gCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTATAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCAccc                                                                               >  2:1691265/1‑93 (MQ=255)
             gCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCAccc                                                                               <  1:1156504/93‑1 (MQ=255)
               aaaTTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCACCCAt                                                                             >  2:617236/1‑93 (MQ=255)
               aaaTTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCAt                                                                             <  1:7076431/93‑1 (MQ=255)
                aaTTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATa                                                                            >  2:1234250/1‑93 (MQ=255)
                aaTTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATa                                                                            >  2:3225246/1‑93 (MQ=255)
                aaTTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATa                                                                            <  1:964263/93‑1 (MQ=255)
                aaTTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATa                                                                            <  1:136127/93‑1 (MQ=255)
                 aTTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATaa                                                                           >  2:10028611/1‑93 (MQ=255)
                    gACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATACAtt                                                                        >  1:1492009/1‑93 (MQ=255)
                    gACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAAtt                                                                        >  1:951188/1‑93 (MQ=255)
                    gACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAAtt                                                                        >  1:5653296/1‑93 (MQ=255)
                      ccGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATttg                                                                      <  2:5013901/93‑1 (MQ=255)
                      ccGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATttg                                                                      >  1:4805313/1‑93 (MQ=255)
                       cGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCACCCATAAATttgt                                                                     >  2:3937376/1‑93 (MQ=255)
                       cGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATttgt                                                                     >  1:4324453/1‑93 (MQ=255)
                       cGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATttgt                                                                     <  2:4010889/93‑1 (MQ=255)
                         aCATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCACCCATAAATttgttg                                                                   >  2:2019270/1‑93 (MQ=255)
                          cATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGc                                                                  >  1:108434/1‑93 (MQ=255)
                           aTACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCt                                                                 >  1:922484/1‑93 (MQ=255)
                           aTACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCt                                                                 <  2:4591723/93‑1 (MQ=255)
                             aCCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCtgt                                                               >  2:7320105/1‑93 (MQ=255)
                              ccATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTGACCCATAAATTTGTTGCtgtt                                                              <  2:6801007/93‑1 (MQ=255)
                              ccATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCtgtt                                                              <  1:3174518/93‑1 (MQ=255)
                               cATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCtgttt                                                             >  1:7312924/1‑93 (MQ=255)
                                aTAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCtgtttg                                                            >  2:6859634/1‑93 (MQ=255)
                                 tAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCtgtttgt                                                           >  2:2115156/1‑93 (MQ=255)
                                 tAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCtgtttgt                                                           >  1:8528671/1‑93 (MQ=255)
                                    aTTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGtttgtttg                                                        >  2:6656218/1‑93 (MQ=255)
                                     ttGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGa                                                       >  1:5943394/1‑93 (MQ=255)
                                       gTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAAt                                                     <  1:7776769/93‑1 (MQ=255)
                                        ttttAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATg                                                    >  1:6364885/1‑93 (MQ=255)
                                         tttAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGt                                                   >  1:4094081/1‑93 (MQ=255)
                                          ttAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCACCCATAGATTTGTTGCTGTTTGTTTGAATGTa                                                  >  2:9711065/1‑93 (MQ=255)
                                          ttAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTa                                                  <  1:3022043/93‑1 (MQ=255)
                                          ttAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTa                                                  <  2:2785692/93‑1 (MQ=255)
                                            aaaaaTAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGc                                                >  1:6934207/1‑93 (MQ=255)
                                             aaaaTAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGcc                                               >  1:4970142/1‑93 (MQ=255)
                                             aaaaTAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGcc                                               <  2:1839825/93‑1 (MQ=255)
                                               aaTAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCa                                             <  2:6547835/93‑1 (MQ=255)
                                                aTAAGCAATCCATTTAAAAAGACGGCCCGCGCGAGAATGTCGTGCCGGAGGGTCAGCCATAAATCTGTTGCTGTTTGTTTGAATGTAGCCCAg                                            >  2:6437687/1‑93 (MQ=255)
                                                aTAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAg                                            <  2:5679875/93‑1 (MQ=255)
                                                aTAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAg                                            >  1:553875/1‑93 (MQ=255)
                                                   aGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGa                                         >  2:4282647/1‑93 (MQ=255)
                                                      aaTCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTc                                      >  2:9133931/1‑93 (MQ=255)
                                                       aTCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCa                                     >  1:8807195/1‑93 (MQ=255)
                                                       aTCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCa                                     >  1:6590157/1‑93 (MQ=255)
                                                       aTCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCa                                     >  1:4032443/1‑93 (MQ=255)
                                                        tCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCAc                                    >  1:5345529/1‑93 (MQ=255)
                                                         ccATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACt                                   <  1:4637467/93‑1 (MQ=255)
                                                          cATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTg                                  <  2:141812/93‑1 (MQ=255)
                                                          cATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTAACTg                                  >  1:3456551/1‑93 (MQ=255)
                                                           aTTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGa                                 <  2:9403912/93‑1 (MQ=255)
                                                              tAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATAc                              <  1:8216209/93‑1 (MQ=255)
                                                               aaaaaGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATAcc                             >  1:3203002/1‑93 (MQ=255)
                                                               aaaaaGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATAcc                             <  1:2334391/93‑1 (MQ=255)
                                                                aaaaGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACct                            >  1:3797542/1‑93 (MQ=255)
                                                                 aaaGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACctc                           <  1:6854015/93‑1 (MQ=255)
                                                                 aaaGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACctc                           <  1:5478699/93‑1 (MQ=255)
                                                                 aaaGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACctc                           <  2:5435372/93‑1 (MQ=255)
                                                                  aaGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACctct                          <  1:1822556/93‑1 (MQ=255)
                                                                  aaGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGGCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACctct                          >  1:7562744/1‑93 (MQ=255)
                                                                   aGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCCGTGACTCACTGATACCTCTg                         >  1:7168529/1‑93 (MQ=255)
                                                                   aGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTg                         >  2:4681875/1‑93 (MQ=255)
                                                                    gACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGt                        >  1:6806534/1‑93 (MQ=255)
                                                                    gACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGt                        >  1:7996204/1‑93 (MQ=255)
                                                                     aCGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTa                       <  1:2547835/93‑1 (MQ=255)
                                                                       ggCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAAc                     >  2:4740687/1‑93 (MQ=255)
                                                                        gCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAAcc                    >  2:5974035/1‑93 (MQ=255)
                                                                        gCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAAcc                    >  2:8633731/1‑93 (MQ=255)
                                                                         ccAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACca                   >  1:6474867/1‑93 (MQ=255)
                                                                            gcgcGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACaa                >  1:6121644/1‑93 (MQ=255)
                                                                            gcgcGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACaa                >  2:8832898/1‑93 (MQ=255)
                                                                            gcgcGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACaa                >  2:2240265/1‑93 (MQ=255)
                                                                             cgcgAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAAt               <  2:1146295/93‑1 (MQ=255)
                                                                               cgAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAAttt             >  1:9361627/1‑93 (MQ=255)
                                                                                gagaATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAAtttt            >  1:9945350/1‑93 (MQ=255)
                                                                                 agaATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTg           >  1:4538798/1‑93 (MQ=255)
                                                                                   aaTGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGtt         >  1:6219904/1‑93 (MQ=255)
                                                                                    aTGTCGCGCCGAAGGGTCACCCGTAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGttt        <  2:9549975/93‑1 (MQ=255)
                                                                                     tGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGTTTa       >  2:921709/1‑93 (MQ=255)
                                                                                      gTCGTGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGTTTaa      >  2:7968017/1‑93 (MQ=255)
                                                                                      gTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGTTTaa      <  1:2971766/93‑1 (MQ=255)
                                                                                       tCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGTTTAAt     >  2:7379993/1‑93 (MQ=255)
                                                                                       tCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGTTTAAt     <  2:7165453/93‑1 (MQ=255)
                                                                                          |                                                                                            
TGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGTTTAATTAT  >  NC_000913/1308145‑1308327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATATGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGTTTAATTATC  >  NC_000913/1308142‑1308328
                                                                                             |                                                                                             
ATATGGAAAATATCAAGCAAATTGACCGACATACCATAGTTTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGC                                                                                               >  SRR4302540.5115840/1‑94 (MQ=60)
ATATGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGC                                                                                               <  SRR4302540.4204946/94‑1 (MQ=60)
ATATGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGC                                                                                               >  SRR4302540.6282325/1‑94 (MQ=60)
ATATGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGC                                                                                               <  SRR4302540.6913346/94‑1 (MQ=60)
ATATGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGC                                                                                               >  SRR4302540.3140297/1‑94 (MQ=60)
ATATGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGC                                                                                               >  SRR4302540.7255938/1‑94 (MQ=60)
 TATGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCG                                                                                              >  SRR4302540.4775379/1‑94 (MQ=60)
 TATGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCG                                                                                              <  SRR4302540.2764582/94‑1 (MQ=60)
  ATGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGC                                                                                             >  SRR4302540.9087354/1‑94 (MQ=60)
   TGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCC                                                                                            >  SRR4302540.9978134/1‑94 (MQ=60)
   TGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCC                                                                                            >  SRR4302540.2751169/1‑94 (MQ=60)
    GGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCG                                                                                           >  SRR4302540.8375279/1‑94 (MQ=60)
     GAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGA                                                                                          >  SRR4302540.9023093/1‑94 (MQ=60)
     GAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGA                                                                                          >  SRR4302540.8371967/1‑94 (MQ=60)
       AAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAG                                                                                        >  SRR4302540.77209/1‑94 (MQ=60)
        AATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGG                                                                                       <  SRR4302540.6222781/94‑1 (MQ=60)
        AATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGG                                                                                       >  SRR4302540.717571/1‑94 (MQ=60)
         ATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGG                                                                                      >  SRR4302540.10107682/1‑94 (MQ=60)
         ATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGG                                                                                      >  SRR4302540.8196225/1‑94 (MQ=60)
          TATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGT                                                                                     >  SRR4302540.1327553/1‑94 (MQ=60)
          TATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGCCCAGCGCGAGAATGTCGTGCCGAAGGGT                                                                                     <  SRR4302540.5882293/94‑1 (MQ=60)
           ATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTC                                                                                    <  SRR4302540.2556811/94‑1 (MQ=60)
             CAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCAC                                                                                  >  SRR4302540.9724214/1‑94 (MQ=60)
               AGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGGGAGAATGTCGCGCCGAAGGGTCACCC                                                                                <  SRR4302540.4936181/94‑1 (MQ=60)
               AGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCC                                                                                <  SRR4302540.4936180/94‑1 (MQ=60)
                GCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCA                                                                               <  SRR4302540.6581371/94‑1 (MQ=60)
                GCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCA                                                                               >  SRR4302540.5847105/1‑94 (MQ=60)
                GCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCA                                                                               <  SRR4302540.1168537/94‑1 (MQ=60)
                ACAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCA                                                                               <  SRR4302540.72265/94‑1 (MQ=60)
                 CAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCAT                                                                              <  SRR4302540.8316960/94‑1 (MQ=60)
                  AAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAACAAGACGGCCAGCGCGAGAATGTCGCGCCGAATGGTCGCCCATA                                                                             >  SRR4302540.1812794/1‑94 (MQ=60)
                  AAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATA                                                                             <  SRR4302540.7155174/94‑1 (MQ=60)
                   AATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAA                                                                            <  SRR4302540.137556/94‑1 (MQ=60)
                   AATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAA                                                                            >  SRR4302540.6885170/1‑94 (MQ=60)
                   AATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAA                                                                            <  SRR4302540.974222/94‑1 (MQ=60)
                    ATTGACCAACATACCATAGATTGTTTTAAACATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCACCCATAAA                                                                           <  SRR4302540.1254719/94‑1 (MQ=60)
                      TGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATACATT                                                                         >  SRR4302540.1508421/1‑94 (MQ=60)
                      TGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATT                                                                         >  SRR4302540.5715537/1‑94 (MQ=60)
                      TGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATT                                                                         >  SRR4302540.961071/1‑94 (MQ=60)
                       GACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTT                                                                        <  SRR4302540.7326805/94‑1 (MQ=60)
                       GACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTT                                                                        <  SRR4302540.7429388/94‑1 (MQ=60)
                        ACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTG                                                                       >  SRR4302540.4856743/1‑94 (MQ=60)
                        ACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTG                                                                       >  SRR4302540.4856742/1‑94 (MQ=60)
                        ACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTG                                                                       >  SRR4302540.6309495/1‑94 (MQ=60)
                         CCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGT                                                                      <  SRR4302540.709386/94‑1 (MQ=60)
                         CCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGT                                                                      >  SRR4302540.4371533/1‑94 (MQ=60)
                           GACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTG                                                                    >  SRR4302540.3007018/1‑94 (MQ=60)
                            ACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGC                                                                   <  SRR4302540.7768988/94‑1 (MQ=60)
                            ACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGC                                                                   >  SRR4302540.5767697/1‑94 (MQ=60)
                            ACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGC                                                                   >  SRR4302540.109597/1‑94 (MQ=60)
                             CATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCACCCATAAATTTGTTGCT                                                                  <  SRR4302540.3046880/94‑1 (MQ=60)
                             CATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCT                                                                  >  SRR4302540.932214/1‑94 (MQ=60)
                             CATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCT                                                                  >  SRR4302540.733400/1‑94 (MQ=60)
                             CATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCAAGCGCGAGAATGTCGCGGCGAAGGGTCACCCATAAATTTGTTGCT                                                                  >  SRR4302540.2774091/1‑94 (MQ=60)
                              ATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTG                                                                 >  SRR4302540.3972940/1‑94 (MQ=60)
                               TACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGT                                                                >  SRR4302540.5330729/1‑94 (MQ=60)
                               TACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGT                                                                >  SRR4302540.627358/1‑94 (MQ=60)
                                ACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTT                                                               <  SRR4302540.4788611/94‑1 (MQ=60)
                                ACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTT                                                               >  SRR4302540.890901/1‑94 (MQ=60)
                                ACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTT                                                               >  SRR4302540.890899/1‑94 (MQ=60)
                                 CCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCACCCATAAATTTGTTGCTGTTT                                                              <  SRR4302540.7028301/94‑1 (MQ=60)
                                 CCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTT                                                              <  SRR4302540.3209028/94‑1 (MQ=60)
                                 CCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTT                                                              >  SRR4302540.7394576/1‑94 (MQ=60)
                                 CCATAGATTATTTTAAAAATANANAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTT                                                              <  SRR4302540.5407935/94‑1 (MQ=60)
                                  CATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTG                                                             >  SRR4302540.5415236/1‑94 (MQ=60)
                                  CATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTG                                                             >  SRR4302540.9912167/1‑94 (MQ=60)
                                   ATAGATTGTTTTAAAAATAAGCCATCCATTTAAAAAGACGGCACGCGCGAGAATCTCGACCCGCAGTGTAACCCATAAATTTGTGTCTGTTTGT                                                            >  SRR4302540.4174038/1‑94 (MQ=32)
                                   ATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGT                                                            >  SRR4302540.8729704/1‑94 (MQ=60)
                                   ATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGT                                                            >  SRR4302540.8627768/1‑94 (MQ=60)
                                   ATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGT                                                            <  SRR4302540.10067814/94‑1 (MQ=60)
                                   ATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGT                                                            >  SRR4302540.3270914/1‑94 (MQ=60)
                                   ATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGT                                                            >  SRR4302540.4174326/1‑94 (MQ=60)
                                    TAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTT                                                           >  SRR4302540.7994574/1‑94 (MQ=60)
                                     AGATTGTTTTAAAGATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCAAAAATTTGTTGCTGTTTGTTT                                                          <  SRR4302540.9059207/94‑1 (MQ=60)
                                     AGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTT                                                          <  SRR4302540.7993819/94‑1 (MQ=60)
                                      GATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTG                                                         >  SRR4302540.10106728/1‑94 (MQ=60)
                                       ATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGA                                                        >  SRR4302540.6009092/1‑94 (MQ=60)
                                       ATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGA                                                        >  SRR4302540.2198393/1‑94 (MQ=60)
                                         TGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAAT                                                      <  SRR4302540.638884/94‑1 (MQ=60)
                                         TGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAAT                                                      <  SRR4302540.56093/94‑1 (MQ=60)
                                         TGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAAT                                                      >  SRR4302540.4094569/1‑94 (MQ=60)
                                         TGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAAT                                                      <  SRR4302540.3534678/94‑1 (MQ=60)
                                          GTTTTAAAAATAAGCAATCCATTTAAAAAGACGTCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATG                                                     <  SRR4302540.7251400/94‑1 (MQ=60)
                                          GTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATG                                                     <  SRR4302540.7867682/94‑1 (MQ=60)
                                          GTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATG                                                     >  SRR4302540.6435752/1‑94 (MQ=60)
                                           TTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGT                                                    >  SRR4302540.4138249/1‑94 (MQ=60)
                                           TTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGT                                                    >  SRR4302540.4138248/1‑94 (MQ=60)
                                           TTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGT                                                    >  SRR4302540.5977714/1‑94 (MQ=60)
                                            TTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTA                                                   >  SRR4302540.9509550/1‑94 (MQ=60)
                                            TTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTA                                                   <  SRR4302540.6438800/94‑1 (MQ=60)
                                             TTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAG                                                  <  SRR4302540.6058499/94‑1 (MQ=60)
                                             TTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAG                                                  <  SRR4302540.3055253/94‑1 (MQ=60)
                                             TTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAG                                                  <  SRR4302540.3055254/94‑1 (MQ=60)
                                              TAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGC                                                 >  SRR4302540.7011378/1‑94 (MQ=60)
                                               AAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCC                                                <  SRR4302540.4867707/94‑1 (MQ=60)
                                               AAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCC                                                >  SRR4302540.5023922/1‑94 (MQ=60)
                                                 AAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCA                                              <  SRR4302540.3392939/94‑1 (MQ=60)
                                                  AATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAG                                             >  SRR4302540.5572346/1‑94 (MQ=60)
                                                  AATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAG                                             >  SRR4302540.559913/1‑94 (MQ=60)
                                                  AATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAG                                             >  SRR4302540.5548498/1‑94 (MQ=60)
                                                   ATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGT                                            <  SRR4302540.550017/94‑1 (MQ=60)
                                                    TAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTG                                           >  SRR4302540.9591355/1‑94 (MQ=60)
                                                     AAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGA                                          >  SRR4302540.4986907/1‑94 (MQ=60)
                                                      AGCAATCCATTTAAAGAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGAC                                         <  SRR4302540.5629795/94‑1 (MQ=60)
                                                        CAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTTGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTTTTTGAGTGTAACCCAGGGACTC                                       >  SRR4302540.7996157/1‑94 (MQ=60)
                                                        CAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTC                                       >  SRR4302540.1383279/1‑94 (MQ=60)
                                                        CAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTC                                       >  SRR4302540.359663/1‑94 (MQ=60)
                                                        CAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTATGAATGTAGCCCAGTGACTC                                       <  SRR4302540.1208426/94‑1 (MQ=60)
                                                         AATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCA                                      >  SRR4302540.4075924/1‑94 (MQ=60)
                                                         AATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCA                                      >  SRR4302540.8909015/1‑94 (MQ=60)
                                                         AATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCA                                      >  SRR4302540.6663149/1‑94 (MQ=60)
                                                         AATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCA                                      >  SRR4302540.2059110/1‑94 (MQ=60)
                                                          ATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCAC                                     >  SRR4302540.6699723/1‑94 (MQ=60)
                                                          ATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCAC                                     >  SRR4302540.5404173/1‑94 (MQ=60)
                                                            CCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTG                                   <  SRR4302540.4687118/94‑1 (MQ=60)
                                                            CCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTG                                   <  SRR4302540.4687125/94‑1 (MQ=60)
                                                            CCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTG                                   <  SRR4302540.9731862/94‑1 (MQ=60)
                                                            CCATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTAACTG                                   >  SRR4302540.3494171/1‑94 (MQ=60)
                                                             CATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGA                                  >  SRR4302540.3450642/1‑94 (MQ=60)
                                                             CATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGA                                  >  SRR4302540.9448217/1‑94 (MQ=60)
                                                              ATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGAT                                 <  SRR4302540.8509166/94‑1 (MQ=60)
                                                              ATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGAT                                 >  SRR4302540.1448997/1‑94 (MQ=60)
                                                              ATTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGCGACTCACTGAT                                 <  SRR4302540.3091179/94‑1 (MQ=60)
                                                               TTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATA                                <  SRR4302540.4108380/94‑1 (MQ=60)
                                                               TTTAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATA                                >  SRR4302540.834194/1‑94 (MQ=60)
                                                                 TAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACC                              >  SRR4302540.5850933/1‑94 (MQ=60)
                                                                 TAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACC                              >  SRR4302540.2592559/1‑94 (MQ=60)
                                                                 TAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACC                              >  SRR4302540.3237790/1‑94 (MQ=60)
                                                                 TAAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACC                              <  SRR4302540.8311704/94‑1 (MQ=60)
                                                                  AAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCT                             >  SRR4302540.3838923/1‑94 (MQ=60)
                                                                  AAAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCT                             <  SRR4302540.2359543/94‑1 (MQ=60)
                                                                   AAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTC                            >  SRR4302540.4998675/1‑94 (MQ=60)
                                                                   AAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTC                            >  SRR4302540.9618622/1‑94 (MQ=60)
                                                                   AAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTC                            >  SRR4302540.6915439/1‑94 (MQ=60)
                                                                   AAAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTC                            <  SRR4302540.8799763/94‑1 (MQ=60)
                                                                    AAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCT                           <  SRR4302540.6929941/94‑1 (MQ=60)
                                                                    AAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCT                           <  SRR4302540.5539010/94‑1 (MQ=60)
                                                                    AAAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGGCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCT                           >  SRR4302540.7647567/1‑94 (MQ=60)
                                                                     AAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCCGTGACTCACTGATACCTCTG                          >  SRR4302540.7248445/1‑94 (MQ=60)
                                                                     AAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTG                          >  SRR4302540.3675979/1‑94 (MQ=60)
                                                                     AAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTG                          >  SRR4302540.3229550/1‑94 (MQ=60)
                                                                     AAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTG                          >  SRR4302540.453650/1‑94 (MQ=60)
                                                                     AAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTG                          >  SRR4302540.453655/1‑94 (MQ=60)
                                                                     AAGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTG                          <  SRR4302540.1842472/94‑1 (MQ=60)
                                                                      AGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGT                         >  SRR4302540.6881850/1‑94 (MQ=60)
                                                                      AGACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGT                         >  SRR4302540.8089283/1‑94 (MQ=60)
                                                                       GACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTA                        >  SRR4302540.694399/1‑94 (MQ=60)
                                                                       GACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTA                        <  SRR4302540.2473191/94‑1 (MQ=60)
                                                                       GACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCAATGATACCTCTGTA                        >  SRR4302540.694400/1‑94 (MQ=60)
                                                                        ACGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAA                       <  SRR4302540.2575296/94‑1 (MQ=60)
                                                                         CGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAAC                      <  SRR4302540.8297683/94‑1 (MQ=60)
                                                                         CGGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAAC                      <  SRR4302540.7141068/94‑1 (MQ=60)
                                                                          CGCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACC                     <  SRR4302540.6015336/94‑1 (MQ=60)
                                                                           GCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCA                    >  SRR4302540.6546912/1‑94 (MQ=60)
                                                                           GCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCA                    <  SRR4302540.8495414/94‑1 (MQ=60)
                                                                           GCCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCA                    <  SRR4302540.26867/94‑1 (MQ=60)
                                                                            CCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCAC                   >  SRR4302540.8880553/1‑94 (MQ=60)
                                                                            CCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCAC                   >  SRR4302540.7406061/1‑94 (MQ=60)
                                                                            CCAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCAC                   >  SRR4302540.9649778/1‑94 (MQ=60)
                                                                             CAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACA                  <  SRR4302540.6803008/94‑1 (MQ=60)
                                                                             CAGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACA                  >  SRR4302540.6487687/1‑94 (MQ=60)
                                                                              AGCGCGAGAATGTCGCGCGGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAA                 <  SRR4302540.6061388/94‑1 (MQ=60)
                                                                              AGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTTTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAA                 <  SRR4302540.1538394/94‑1 (MQ=60)
                                                                              AGCGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAA                 >  SRR4302540.6189825/1‑94 (MQ=60)
                                                                                CGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATT               <  SRR4302540.5663830/94‑1 (MQ=60)
                                                                                CGCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATT               <  SRR4302540.1134885/94‑1 (MQ=60)
                                                                                 GCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTT              <  SRR4302540.3842870/94‑1 (MQ=60)
                                                                                 GCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTT              >  SRR4302540.4568568/1‑94 (MQ=60)
                                                                                 GCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTT              >  SRR4302540.9473109/1‑94 (MQ=60)
                                                                                 GCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTT              <  SRR4302540.6658382/94‑1 (MQ=60)
                                                                                 GCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTT              >  SRR4302540.7544902/1‑94 (MQ=60)
                                                                                 GCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTT              >  SRR4302540.2961631/1‑94 (MQ=60)
                                                                                 GCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTT              >  SRR4302540.6905375/1‑94 (MQ=60)
                                                                                 GCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTT              <  SRR4302540.3193442/94‑1 (MQ=60)
                                                                                 GCGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTT              >  SRR4302540.3212656/1‑94 (MQ=60)
                                                                                  CGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGTCCAGTGACTCATTGATACCTCTataagcccactttt             >  SRR4302540.5322277/1‑80 (MQ=60)
                                                                                  CGAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTT             >  SRR4302540.10061487/1‑94 (MQ=60)
                                                                                   GAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTG            >  SRR4302540.4587922/1‑94 (MQ=60)
                                                                                   GAGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTG            <  SRR4302540.9409267/94‑1 (MQ=60)
                                                                                    AGAATGTCGTGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGT           <  SRR4302540.4668171/94‑1 (MQ=60)
                                                                                    AGAATGTCGTGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGT           <  SRR4302540.4667971/94‑1 (MQ=60)
                                                                                    AGAATGTCGTGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGT           >  SRR4302540.6358244/1‑94 (MQ=60)
                                                                                    AGAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGT           >  SRR4302540.6563971/1‑94 (MQ=60)
                                                                                     GAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGTT          >  SRR4302540.1681120/1‑94 (MQ=60)
                                                                                     GAATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGTT          >  SRR4302540.6289098/1‑94 (MQ=60)
                                                                                       ATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGTTTA        >  SRR4302540.5953264/1‑94 (MQ=60)
                                                                                       ATGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGTTTA        <  SRR4302540.9232207/94‑1 (MQ=60)
                                                                                        TGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGTTTAT       >  SRR4302540.8071182/1‑94 (MQ=60)
                                                                                        TGTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGTTTAA       >  SRR4302540.2484820/1‑94 (MQ=60)
                                                                                         GTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGTTTAAT      <  SRR4302540.5023850/94‑1 (MQ=60)
                                                                                         GTCGCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGTTTAAT      <  SRR4302540.3004590/94‑1 (MQ=60)
                                                                                         GTCGCGCCGAAGCGTCACCGATAAATGTGTTGCGGTTGTTGTGAATGTAGCCCAGGTACTCACTGATCCCTCTGTAACCACAATTTTGTTTAAT      <  SRR4302540.3004313/94‑1 (MQ=32)
                                                                                            GCGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGTTTAATTAT   <  SRR4302540.1561389/94‑1 (MQ=60)
                                                                                             TGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGTTTAATTATC  <  SRR4302540.1145577/94‑1 (MQ=60)
                                                                                             CGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGTTTAATTATC  >  SRR4302540.9711823/1‑94 (MQ=60)
                                                                                             |                                                                                             
ATATGGAAAATATCAAGCAAATTGACCGACATACCATAGATTGTTTTAAAAATAAGCAATCCATTTAAAAAGACGGCCAGCGCGAGAATGTCGTGCCGAAGGGTCACCCATAAATTTGTTGCTGTTTGTTTGAATGTAGCCCAGTGACTCACTGATACCTCTGTAACCACAATTTTGTTTAATTATC  >  NC_000913/1308142‑1308328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑