Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,371,769 T→C F175S (TTC→TCC)  ycjO → putative binding‑protein dependent transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,371,7690TC100.0% 561.0 / NA 173F175S (TTC→TCC) ycjOputative binding‑protein dependent transport protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (81/92);  total (81/92)

Only 100 of 183 total aligned reads displayed.

TTCTGCATCTCTATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTTCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGGCGCTAATGCCT  >  NC_000913/1371679‑1371858
                                                                                          |                                                                                         
ttctGCATCTCTATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTccc                                                                                         >  1:4937410/1‑93 (MQ=255)
ttctGCATCTCTATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTccc                                                                                         <  1:3734194/93‑1 (MQ=255)
ttctGCATCTCTATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTccc                                                                                         <  2:5407109/93‑1 (MQ=255)
 tctGCATCTCTATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTcccc                                                                                        >  1:3004206/1‑93 (MQ=255)
   tGCATCTCTATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCTATCTGGCGCTACTCCCCGt                                                                                      <  2:6149869/93‑1 (MQ=255)
    gCATCTCTATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTa                                                                                     <  2:3475038/93‑1 (MQ=255)
    gCATCTCTATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTa                                                                                     <  2:567018/93‑1 (MQ=255)
     cATCTCTATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTAt                                                                                    >  1:157932/1‑93 (MQ=255)
      aTCTCTATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATg                                                                                   >  2:1800574/1‑93 (MQ=255)
       tctcTATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGc                                                                                  >  1:6050826/1‑93 (MQ=255)
         tctATGAGCAGGCACCGCTGTGGTTGGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCt                                                                                >  1:5856518/1‑93 (MQ=255)
         tctATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCt                                                                                <  1:379212/93‑1 (MQ=255)
          ctATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCtt                                                                               <  1:1772781/93‑1 (MQ=255)
           tATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCttt                                                                              >  2:4155440/1‑93 (MQ=255)
           tATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCttt                                                                              <  2:3180094/93‑1 (MQ=255)
            aTGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTa                                                                             <  1:2359899/93‑1 (MQ=255)
            aTGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTa                                                                             <  2:4079008/93‑1 (MQ=255)
            aTGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTa                                                                             <  1:1969295/93‑1 (MQ=255)
              gAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTAtc                                                                           >  2:4796982/1‑93 (MQ=255)
              gAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTAtc                                                                           <  2:4952484/93‑1 (MQ=255)
               aGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTAtct                                                                          <  2:6210131/93‑1 (MQ=255)
                gCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTAtctc                                                                         >  2:2956184/1‑93 (MQ=255)
                 cAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCg                                                                        <  1:4515781/93‑1 (MQ=255)
                  aGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGt                                                                       <  2:5312741/93‑1 (MQ=255)
                  aGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGt                                                                       >  2:5416625/1‑93 (MQ=255)
                   ggCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTGTCTCGtt                                                                      >  2:2913621/1‑93 (MQ=255)
                   ggCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCggt                                                                      >  1:6201866/1‑91 (MQ=255)
                   ggCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGtt                                                                      >  2:1497098/1‑93 (MQ=255)
                     cACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTc                                                                    <  2:1181151/93‑1 (MQ=255)
                     cACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTc                                                                    >  2:4754687/1‑93 (MQ=255)
                      aCCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCt                                                                   >  1:5109932/1‑93 (MQ=255)
                       ccGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTg                                                                  <  1:3386187/93‑1 (MQ=255)
                       ccGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTg                                                                  >  1:5047032/1‑93 (MQ=255)
                         gCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGc                                                                >  2:2557187/1‑93 (MQ=255)
                           tgtgGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGa                                                              >  2:1308048/1‑93 (MQ=255)
                             tgGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCCTCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGAtt                                                            >  2:2603219/1‑93 (MQ=255)
                              ggTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGAttt                                                           <  2:2249313/93‑1 (MQ=255)
                               gTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGAtttt                                                          >  2:698669/1‑93 (MQ=255)
                               gTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGAtttt                                                          <  1:2903502/93‑1 (MQ=255)
                                 tCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGc                                                        <  1:5647859/93‑1 (MQ=255)
                                  cGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCa                                                       <  1:3935919/93‑1 (MQ=255)
                                  cGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCa                                                       <  2:1479670/93‑1 (MQ=255)
                                   gATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAg                                                      >  1:4596638/1‑93 (MQ=255)
                                    aTAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGa                                                     <  1:1269165/93‑1 (MQ=255)
                                    aTAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGa                                                     >  2:2933675/1‑93 (MQ=255)
                                    aTAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGa                                                     >  1:2538059/1‑93 (MQ=255)
                                     tAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGAc                                                    >  1:2620049/1‑93 (MQ=255)
                                     tAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGAc                                                    >  1:890426/1‑93 (MQ=255)
                                       aTCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCa                                                  >  2:3153224/1‑93 (MQ=255)
                                       aTCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCa                                                  <  1:251709/93‑1 (MQ=255)
                                        tacGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCAt                                                 >  2:1543496/3‑93 (MQ=255)
                                        tCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCAt                                                 <  1:4212574/93‑1 (MQ=255)
                                        tCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCAt                                                 <  2:6217101/93‑1 (MQ=255)
                                           gggCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGa                                              >  2:4383749/1‑93 (MQ=255)
                                            ggCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGAc                                             <  2:4134470/93‑1 (MQ=255)
                                             gCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACa                                            <  2:4251378/93‑1 (MQ=255)
                                                gTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAAt                                         >  2:2053932/1‑93 (MQ=255)
                                                 tAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATc                                        >  1:3069105/1‑93 (MQ=255)
                                                   gCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGc                                      >  1:1141000/1‑93 (MQ=255)
                                                    cTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCt                                     <  2:3512855/93‑1 (MQ=255)
                                                     tttGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTg                                    <  1:5242027/93‑1 (MQ=255)
                                                      ttGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGt                                   <  2:3875414/93‑1 (MQ=255)
                                                        gtgCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTAc                                 <  1:1113402/93‑1 (MQ=255)
                                                        gtgCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTAc                                 <  2:1093304/93‑1 (MQ=255)
                                                          gCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGa                               <  1:1061652/93‑1 (MQ=255)
                                                           cTGTTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGaa                              <  2:188875/93‑1 (MQ=255)
                                                              gtggtgCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCg                           <  1:1577899/93‑1 (MQ=255)
                                                                ggtgCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGc                         >  1:5418629/1‑93 (MQ=255)
                                                                ggtgCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGc                         <  1:3359489/93‑1 (MQ=255)
                                                                ggtgCTGGTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGc                         >  2:6196487/1‑93 (MQ=255)
                                                                 gtgCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCa                        <  1:5168443/93‑1 (MQ=255)
                                                                 gtgCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCa                        >  2:4999209/1‑93 (MQ=255)
                                                                   gCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCaga                      <  1:5908232/93‑1 (MQ=255)
                                                                   gCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCaga                      <  1:1446807/93‑1 (MQ=255)
                                                                     cgTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAgaga                    <  2:5725017/92‑1 (MQ=255)
                                                                      gTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAgat                   <  1:659253/93‑1 (MQ=255)
                                                                      gTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAgat                   <  1:3338655/93‑1 (MQ=255)
                                                                       ttCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAgatg                  >  2:5782041/1‑93 (MQ=255)
                                                                       ttCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAgatg                  >  1:3155246/1‑93 (MQ=255)
                                                                         cGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAgatgga                <  1:3452519/93‑1 (MQ=255)
                                                                          gCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAgatggat               >  2:4584775/1‑93 (MQ=255)
                                                                           ccATCTTGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAgatggatg              <  1:2084410/93‑1 (MQ=255)
                                                                             aTCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGCAGCGGCAGAGCTGGATGgc            >  2:5519064/1‑93 (MQ=255)
                                                                             aTCTGGCGCTACTCCCCGTATGCCTTTAGCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGgc            <  1:5695427/93‑1 (MQ=255)
                                                                              tCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGgcg           >  2:3694648/1‑93 (MQ=255)
                                                                              tCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGgcg           >  1:1914137/1‑93 (MQ=255)
                                                                               cTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGgcgc          <  2:3758337/93‑1 (MQ=255)
                                                                                  gcgcTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGGCGCTaa       >  1:4873494/1‑93 (MQ=255)
                                                                                  gcgcTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGGCGCTaa       <  1:5048169/93‑1 (MQ=255)
                                                                                  gcgcTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGGCGCTaa       >  1:3664021/1‑93 (MQ=255)
                                                                                  gcgcTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGGCGCTaa       <  2:3460399/93‑1 (MQ=255)
                                                                                  gcgcTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGGCGCTaa       <  1:1710500/93‑1 (MQ=255)
                                                                                  gcgcTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGGCGCTaa       >  2:1617358/1‑93 (MQ=255)
                                                                                  gcgcTACTCCCCGTATGCCTTTATCTCGTCTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGGCGCTaa       >  1:4157546/1‑93 (MQ=255)
                                                                                   cgcTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGGCGCTAAt      <  1:955623/93‑1 (MQ=255)
                                                                                    gcTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGGCGCTAATg     >  1:214016/1‑93 (MQ=255)
                                                                                     cTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGGCGCTAATGc    <  2:772306/93‑1 (MQ=255)
                                                                                     cTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGGCGCTAATGc    >  2:3236879/1‑93 (MQ=255)
                                                                                      tACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGGCGCTAATGcc   >  2:5102026/1‑93 (MQ=255)
                                                                                       aCTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGGCGCTAATGCCt  <  2:1698751/93‑1 (MQ=255)
                                                                                          |                                                                                         
TTCTGCATCTCTATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTTCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGGCGCTAATGCCT  >  NC_000913/1371679‑1371858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCTTCTGCATCTCTATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTTCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGGCGCTAATGCCTGG  >  NC_000913/1371676‑1371860
                                                                                             |                                                                                           
ATCTTCTGCATCTCTATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTC                                                                                             >  SRR4302533.2176550/1‑94 (MQ=60)
ATCTTCTGCATCTCTATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTC                                                                                             >  SRR4302533.4593774/1‑94 (MQ=60)
ATCTTCTGCATCTCTATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTC                                                                                             >  SRR4302533.922217/1‑94 (MQ=60)
ATCTTCTGCATCTCTATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTC                                                                                             >  SRR4302533.1857024/1‑94 (MQ=60)
ATCTCCTGCATCTCTATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTC                                                                                             <  SRR4302533.3973612/94‑1 (MQ=60)
  CTTCTGCATCTCTATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCC                                                                                           >  SRR4302533.4967302/1‑94 (MQ=60)
  CTTCTGCATCTCTATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCC                                                                                           <  SRR4302533.162849/94‑1 (MQ=60)
   TTCTGCATCTCTATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCC                                                                                          <  SRR4302533.3757098/94‑1 (MQ=60)
   TTCTGCATCTCTATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCC                                                                                          >  SRR4302533.3022582/1‑94 (MQ=60)
       cgatctgctgtatCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTAT                                                                                      <  SRR4302533.2470022/81‑1 (MQ=60)
       GCATCTCTATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTAT                                                                                      >  SRR4302533.158938/1‑94 (MQ=60)
       GCATCTCTATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTAT                                                                                      <  SRR4302533.1737410/94‑1 (MQ=60)
       GCATCTCTATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTAT                                                                                      <  SRR4302533.201496/94‑1 (MQ=60)
         ATCTCTATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGC                                                                                    >  SRR4302533.5485572/1‑94 (MQ=60)
         ATCTCTATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGC                                                                                    >  SRR4302533.6087480/1‑94 (MQ=60)
           CTCTATGAGCAGGCACCGCTGTGGTTGGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCT                                                                                  >  SRR4302533.5891923/1‑94 (MQ=60)
            TCTATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTT                                                                                 <  SRR4302533.4586466/94‑1 (MQ=60)
            TCTATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTT                                                                                 <  SRR4302533.381498/94‑1 (MQ=60)
             CTATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTT                                                                                <  SRR4302533.1783578/94‑1 (MQ=60)
               ATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTAT                                                                              >  SRR4302533.5909788/1‑94 (MQ=60)
               ATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTAT                                                                              <  SRR4302533.1981337/94‑1 (MQ=60)
               ATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTAT                                                                              <  SRR4302533.1981338/94‑1 (MQ=60)
               ATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTAT                                                                              <  SRR4302533.2374482/94‑1 (MQ=60)
               ATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCAATCTGGCGCTACTCCCCGTATGCCTTTAT                                                                              <  SRR4302533.3086415/94‑1 (MQ=60)
                  AGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTC                                                                           <  SRR4302533.2714715/94‑1 (MQ=60)
                    CAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGT                                                                         <  SRR4302533.4543126/94‑1 (MQ=60)
                     AGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGGT                                                                        >  SRR4302533.6239063/1‑94 (MQ=60)
                      GGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTT                                                                       <  SRR4302533.2546335/94‑1 (MQ=60)
                       GCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTC                                                                      >  SRR4302533.4991847/1‑94 (MQ=60)
                        CACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCT                                                                     >  SRR4302533.5140812/1‑94 (MQ=60)
                         ACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTG                                                                    >  SRR4302533.5077562/1‑94 (MQ=60)
                          CCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGG                                                                   <  SRR4302533.2320768/94‑1 (MQ=60)
                          CCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGG                                                                   <  SRR4302533.3407095/94‑1 (MQ=60)
                             CTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGA                                                                <  SRR4302533.3897267/94‑1 (MQ=60)
                               GTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATT                                                              <  SRR4302533.1523924/94‑1 (MQ=60)
                                  GTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTG                                                           <  SRR4302533.2921335/94‑1 (MQ=60)
                                  GTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTG                                                           <  SRR4302533.3753391/94‑1 (MQ=60)
                                    TCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCA                                                         <  SRR4302533.5682001/94‑1 (MQ=60)
                                     CGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAG                                                        <  SRR4302533.3959949/94‑1 (MQ=60)
                                     CGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAG                                                        >  SRR4302533.4624484/1‑94 (MQ=60)
                                      GATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGA                                                       >  SRR4302533.901162/1‑94 (MQ=60)
                                      GATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGA                                                       >  SRR4302533.2553684/1‑94 (MQ=60)
                                       ATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGAC                                                      >  SRR4302533.2636132/1‑94 (MQ=60)
                                       ATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGAC                                                      >  SRR4302533.895657/1‑94 (MQ=60)
                                       ATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGAC                                                      <  SRR4302533.1276761/94‑1 (MQ=60)
                                        TAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACC                                                     >  SRR4302533.5271190/1‑94 (MQ=60)
                                          ATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCAT                                                   <  SRR4302533.253259/94‑1 (MQ=60)
                                           TCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATC                                                  <  SRR4302533.4238056/94‑1 (MQ=60)
                                           TCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATC                                                  <  SRR4302533.5774424/94‑1 (MQ=60)
                                              GGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGAC                                               >  SRR4302533.5983123/1‑94 (MQ=60)
                                                GCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAA                                             <  SRR4302533.6319410/94‑1 (MQ=60)
                                                   GTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATC                                          >  SRR4302533.3087950/1‑94 (MQ=60)
                                                    TAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCG                                         <  SRR4302533.4790066/94‑1 (MQ=60)
                                                     AGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGC                                        >  SRR4302533.1147796/1‑94 (MQ=60)
                                                       CTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTG                                      <  SRR4302533.3394232/94‑1 (MQ=60)
                                                        TTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGT                                     <  SRR4302533.5273536/94‑1 (MQ=60)
                                                           GTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACG                                  <  SRR4302533.1120005/94‑1 (MQ=60)
                                                            TGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGA                                 <  SRR4302533.3360699/94‑1 (MQ=60)
                                                             GCTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAA                                <  SRR4302533.1067890/94‑1 (MQ=60)
                                                              CTGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCTTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAG                               <  SRR4302533.3187704/94‑1 (MQ=60)
                                                               TGGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGC                              <  SRR4302533.1863337/94‑1 (MQ=60)
                                                                GGTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCG                             >  SRR4302533.979856/1‑94 (MQ=60)
                                                                 GTGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGG                            <  SRR4302533.1587399/94‑1 (MQ=60)
                                                                  TGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGC                           >  SRR4302533.5451225/1‑94 (MQ=60)
                                                                  TGGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGC                           <  SRR4302533.3159087/94‑1 (MQ=60)
                                                                   GGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCA                          >  SRR4302533.515000/1‑94 (MQ=60)
                                                                   GGTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCA                          <  SRR4302533.3380245/94‑1 (MQ=60)
                                                                    GTGCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAG                         <  SRR4302533.5199640/94‑1 (MQ=60)
                                                                      GCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAG                       <  SRR4302533.1455538/94‑1 (MQ=60)
                                                                      GCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAG                       <  SRR4302533.5943921/94‑1 (MQ=60)
                                                                      GCTGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAG                       >  SRR4302533.4916178/1‑94 (MQ=60)
                                                                        TGTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGAT                     >  SRR4302533.1235260/1‑94 (MQ=60)
                                                                         GTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATG                    <  SRR4302533.663136/94‑1 (MQ=60)
                                                                         GTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATG                    <  SRR4302533.3359289/94‑1 (MQ=60)
                                                                         GTTCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATG                    >  SRR4302533.3174644/1‑94 (MQ=60)
                                                                           TCGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGA                  <  SRR4302533.2572963/94‑1 (MQ=60)
                                                                            CGCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGAT                 <  SRR4302533.3473835/94‑1 (MQ=60)
                                                                             GCCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATG                >  SRR4302533.3543100/1‑94 (MQ=60)
                                                                              CCATCTTGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGG               <  SRR4302533.2097243/94‑1 (MQ=60)
                                                                              CCATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGG               >  SRR4302533.5285719/1‑94 (MQ=60)
                                                                               CATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGGC              >  SRR4302533.3802349/1‑94 (MQ=60)
                                                                                ATCTGGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGGCG             >  SRR4302533.1925820/1‑94 (MQ=60)
                                                                                ATCTGGCGCTACTCCCCGTATGCCTTTAGCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGGCG             <  SRR4302533.5729836/94‑1 (MQ=60)
                                                                                    GGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGGCGCTAA         >  SRR4302533.3686545/1‑94 (MQ=60)
                                                                                    GGCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGGCGCTAA         >  SRR4302533.4903024/1‑94 (MQ=60)
                                                                                    GGCGCTACTCCCCGTATGCCTTTATCTCGTCTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGGCGCTAA         >  SRR4302533.4182737/1‑94 (MQ=60)
                                                                                     GCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGGCGCTAAT        <  SRR4302533.5078705/94‑1 (MQ=60)
                                                                                     GCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGGCGCTAAT        <  SRR4302533.6105920/94‑1 (MQ=60)
                                                                                     GCGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGGCGCTAAT        <  SRR4302533.1720866/94‑1 (MQ=60)
                                                                                      CGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGGCGCTAATG       <  SRR4302533.961235/94‑1 (MQ=60)
                                                                                      CGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGGCGCTAATG       <  SRR4302533.4005944/94‑1 (MQ=60)
                                                                                      CGCTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGGCGCTAATG       >  SRR4302533.215362/1‑94 (MQ=60)
                                                                                       GCTCCTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGGCGCTAATGC      <  SRR4302533.5082304/94‑1 (MQ=60)
                                                                                        CTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGGCGCTAATGCC     >  SRR4302533.1409374/1‑94 (MQ=60)
                                                                                        CTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGGCGCTAATGCC     >  SRR4302533.1142730/1‑94 (MQ=60)
                                                                                        CTACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGGCGCTAATGCC     >  SRR4302533.4323750/1‑94 (MQ=60)
                                                                                         TACTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGGCGCTAATGCCT    >  SRR4302533.1644305/1‑94 (MQ=60)
                                                                                         TACTCACCGTATGCCTTTATCTCGCTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGGCGCTAATGCCT    <  SRR4302533.2027234/94‑1 (MQ=60)
                                                                                           CTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGGTGGCGCTAATGCCTGG  >  SRR4302533.3956468/1‑94 (MQ=60)
                                                                                           CTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGGCGCTAATGCCTGG  >  SRR4302533.1261427/1‑94 (MQ=60)
                                                                                           CTCCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGGCGCTAATGCCTGG  <  SRR4302533.2859112/94‑1 (MQ=60)
                                                                                             |                                                                                           
ATCTTCTGCATCTCTATGAGCAGGCACCGCTGTGGTTCGATAATCCGGGCAGTAGCTTTGTGCTGGTGGTGCTGTTCGCCATCTGGCGCTACTTCCCGTATGCCTTTATCTCGTTTCTGGCGATTTTGCAGACCATCGACAAATCGCTGTACGAAGCGGCAGAGATGGATGGCGCTAATGCCTGG  >  NC_000913/1371676‑1371860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑