Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,456,531	A→G	M82V (ATG→GTG)	b1394 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,456,531	0	A	G	98.9%	564.5 / NA	176	M82V (ATG→GTG)	b1394	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (78/96); minor base T (1/1); total (79/97)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 184 total aligned reads displayed.

TTACTTACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCA  >  NC_000913/1456441‑1456620

  tcttacAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTc                                                                                       <  2:5634986/92‑1 (MQ=255)

  acttacAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTc                                                                                       >  1:3311315/1‑93 (MQ=255)

  acttacAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTc                                                                                       >  1:6163334/1‑93 (MQ=255)

  acttacAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTc                                                                                       >  1:4535272/1‑93 (MQ=255)

   cttacAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCa                                                                                      <  2:1015091/93‑1 (MQ=255)

    ttacAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGTCCCCGCACCGGATTTAGGGGTGTCAg                                                                                     <  1:3043867/93‑1 (MQ=255)

      acAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGtt                                                                                   <  1:1698701/93‑1 (MQ=255)

      acAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGtt                                                                                   <  1:4767476/93‑1 (MQ=255)

      acAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGAATTTAGGGGTGTCAGtt                                                                                   <  1:5774762/93‑1 (MQ=255)

         ggTGCCGGGGGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGGGTCGGTTGaa                                                                                >  2:3806640/1‑93 (MQ=255)

         ggTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGTCCCGCACCGGATTTAGGGGTGTCAGTTGaa                                                                                >  2:776545/1‑93 (MQ=255)

         ggTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGaa                                                                                >  2:3672338/1‑93 (MQ=255)

             ccGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGtt                                                                            <  2:4474774/93‑1 (MQ=255)

               gggCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTc                                                                          >  1:4376443/1‑93 (MQ=255)

                  cgcgGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCtat                                                                       <  2:3233437/93‑1 (MQ=255)

                  cgcgGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCtat                                                                       >  2:3589468/1‑93 (MQ=255)

                  cgcgAATTTTGTGCT‑GTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCtata                                                                      <  2:1957567/93‑1 (MQ=255)

                    cgGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATaa                                                                     <  2:822526/93‑1 (MQ=255)

                    cgGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATaa                                                                     >  1:5256581/1‑93 (MQ=255)

                    cgGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATaa                                                                     >  1:3993225/1‑93 (MQ=255)

                     ggATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAAc                                                                    <  1:1615779/93‑1 (MQ=255)

                     ggATTTTGTGCTGGTCAGGATCTGAACGATCGTAACGTTGATCCAACAGTCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAAc                                                                    <  2:3935194/93‑1 (MQ=255)

                       aTTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAAccc                                                                  >  2:5808450/1‑93 (MQ=255)

                       aTTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAAccc                                                                  <  2:6007877/93‑1 (MQ=255)

                        ttttGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCa                                                                 >  2:3759522/1‑93 (MQ=255)

                        ttttGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCa                                                                 >  1:1236415/1‑93 (MQ=255)

                        ttttGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCa                                                                 <  2:4537137/93‑1 (MQ=255)

                        ttttGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCa                                                                 <  1:1588636/93‑1 (MQ=255)

                        ttttGT‑CTGGGTAGGGTCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCAc                                                                <  1:3159009/93‑1 (MQ=255)

                         tttGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCAc                                                                <  1:6264704/93‑1 (MQ=255)

                         tttGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCAc                                                                >  1:3954223/1‑93 (MQ=255)

                           tgtgCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTg                                                              >  1:679018/1‑93 (MQ=255)

                            gtgCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTgg                                                             <  1:5419606/93‑1 (MQ=255)

                              gCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTa                                                           >  1:1361326/1‑93 (MQ=255)

                                 ggTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGt                                                        <  1:5604312/93‑1 (MQ=255)

                                 ggTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGt                                                        <  2:2466357/93‑1 (MQ=255)

                                 ggTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGt                                                        <  1:765308/93‑1 (MQ=255)

                                 ggTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGt                                                        <  1:2282231/93‑1 (MQ=255)

                                 ggTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGt                                                        <  1:2598536/93‑1 (MQ=255)

                                 ggTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGt                                                        <  2:117519/93‑1 (MQ=255)

                                 ggTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGt                                                        <  2:5012156/93‑1 (MQ=255)

                                 ggTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGAGTCAGATGAACGGTTCTATAACCCACTGTTAcac                                                        >  2:1743877/1‑91 (MQ=255)

                                  gTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTc                                                       >  2:4450286/1‑93 (MQ=255)

                                  gTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTc                                                       <  2:4379375/93‑1 (MQ=255)

                                    cAGGATCTTAACGATCGTAACGTTGATCCCACAGGACCCGCAGCGGATTTAGGGTTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGc                                                     >  1:5676787/1‑93 (MQ=255)

                                     aGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGcc                                                    <  1:3978820/93‑1 (MQ=255)

                                     aGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGcc                                                    >  2:5504452/1‑93 (MQ=255)

                                      ggATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCt                                                   <  1:91913/93‑1 (MQ=255)

                                      ggATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCt                                                   <  1:1043540/93‑1 (MQ=255)

                                      ggATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCt                                                   <  2:954878/93‑1 (MQ=255)

                                       gATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTg                                                  <  1:4633943/93‑1 (MQ=255)

                                         tCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGc                                                >  1:40680/1‑93 (MQ=255)

                                         tCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGc                                                >  1:1437304/1‑93 (MQ=255)

                                         tCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAAACCACTGGTACGTCGCCTGGc                                                >  2:4796168/1‑93 (MQ=255)

                                           ttAACGATCGTAATGTTGCTCCCACAGGCCCCGCACCGGATTTAAGGGCGTCAGTTGAATGATTCTATAACCCACTGGTACGTCGCCTGGcca                                              >  2:2104898/1‑91 (MQ=255)

                                            tAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCaaa                                             >  2:11461/1‑93 (MQ=255)

                                             aaCGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCaaaa                                            <  2:5614135/93‑1 (MQ=255)

                                                gATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTg                                         <  2:4163205/93‑1 (MQ=255)

                                                 aTCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGc                                        >  1:3441303/1‑93 (MQ=255)

                                                    gTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCaa                                     >  1:5438288/1‑93 (MQ=255)

                                                     tAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCaca                                    >  1:4422485/1‑91 (MQ=255)

                                                       aCGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAAc                                  <  2:544979/93‑1 (MQ=255)

                                                       aCGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAAc                                  <  1:4735181/93‑1 (MQ=255)

                                                       aCGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAAc                                  <  2:2932014/93‑1 (MQ=255)

                                                         gTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCg                                >  1:962220/1‑93 (MQ=255)

                                                         gTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCg                                >  2:4053398/1‑93 (MQ=255)

                                                          ttGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCgg                               >  1:1049876/1‑93 (MQ=255)

                                                          ttGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCgg                               >  2:2051258/1‑93 (MQ=255)

                                                          ttGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCgg                               >  2:5863416/1‑93 (MQ=255)

                                                          ttGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCgg                               >  1:1206691/1‑93 (MQ=255)

                                                           tGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGt                              <  2:1298957/93‑1 (MQ=255)

                                                              tCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGAt                           >  2:4939164/1‑93 (MQ=255)

                                                                ccACAGGCCCCGCACCGGATTTAGGGGTTTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTAATCt                         <  2:3980043/93‑1 (MQ=255)

                                                                ccACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCt                         <  2:3357321/93‑1 (MQ=255)

                                                                ccACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCt                         <  1:5054120/93‑1 (MQ=255)

                                                                 cacaGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCtg                        <  1:4835837/93‑1 (MQ=255)

                                                                 cacaGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCtg                        >  1:2924584/1‑93 (MQ=255)

                                                                 cacaGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCtg                        <  1:491834/93‑1 (MQ=255)

                                                                  acaGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCtgt                       >  2:4347097/1‑93 (MQ=255)

                                                                   caGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCtgtg                      >  2:3141553/1‑93 (MQ=255)

                                                                    aGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTAGCAAAACTGCCAAAACCGGTGATCTGTGc                     <  1:5797543/93‑1 (MQ=255)

                                                                     ggCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCa                    <  1:4371979/93‑1 (MQ=255)

                                                                     ggCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTGTCTGTAATCCACTGGTACGTCGCCTGGCAGAACGGACAAAACCGGTGATCTGTGCa                    >  2:4171206/1‑93 (MQ=255)

                                                                       ccccGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGt                  >  1:4476191/1‑93 (MQ=255)

                                                                           gCATCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAt              <  2:4122673/93‑1 (MQ=255)

                                                                           gCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAt              <  1:2150652/93‑1 (MQ=255)

                                                                           gCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAt              <  2:1211126/93‑1 (MQ=255)

                                                                           gCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAAACCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAt              >  2:2240879/1‑93 (MQ=255)

                                                                            cACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCTGTGATCTGTGCAGTCAAtg             <  2:6126876/93‑1 (MQ=255)

                                                                            cACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtg             >  2:3639573/1‑93 (MQ=255)

                                                                              ccGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggc           >  2:5959528/1‑93 (MQ=255)

                                                                               cGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggcg          >  2:2002937/1‑93 (MQ=255)

                                                                               cGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggcg          <  2:5396199/93‑1 (MQ=255)

                                                                                ggATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggcgt         <  1:3115283/93‑1 (MQ=255)

                                                                                ggATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggcgt         >  1:2894189/1‑93 (MQ=255)

                                                                                ggATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggcgt         <  1:3775277/93‑1 (MQ=255)

                                                                                ggATTTAGGGGTGTCAGTTGAACGTTACTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggcgt         <  2:2705456/93‑1 (MQ=255)

                                                                                   tttAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggcgtggc      <  2:177159/93‑1 (MQ=255)

                                                                                       ggggTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCggca  >  2:2592651/1‑93 (MQ=255)

                                                                                       ggggTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCggca  <  1:3406867/93‑1 (MQ=255)

TTACTTACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCA  >  NC_000913/1456441‑1456620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTGTTACTTACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGG  >  NC_000913/1456438‑1456624

CTGTTACTTACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCGACAGGTCCCGCACCGGATTTAGGGG                                                                                               >  SRR4302533.3202828/1‑94 (MQ=60)

CTGTTACTTACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGG                                                                                               <  SRR4302533.1522484/94‑1 (MQ=60)

  GTTACTTACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTG                                                                                             <  SRR4302533.3017333/94‑1 (MQ=60)

    TACTTACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTC                                                                                           >  SRR4302533.4562733/1‑94 (MQ=60)

    TACTTACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTC                                                                                           >  SRR4302533.3331763/1‑94 (MQ=60)

    TACTTACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTC                                                                                           >  SRR4302533.6200390/1‑94 (MQ=60)

     ACTTACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCA                                                                                          >  SRR4302533.4009183/1‑94 (MQ=60)

      CTTACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAG                                                                                         <  SRR4302533.5128868/94‑1 (MQ=60)

       TTACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGTCCCCGCACCGGATTTAGGGGTGTCAGT                                                                                        <  SRR4302533.3062499/94‑1 (MQ=60)

         ACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTG                                                                                      <  SRR4302533.1708997/94‑1 (MQ=60)

         ACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTG                                                                                      <  SRR4302533.4796377/94‑1 (MQ=60)

         ACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGAATTTAGGGGTGTCAGTTG                                                                                      <  SRR4302533.5809635/94‑1 (MQ=60)

                CCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTT                                                                               <  SRR4302533.669933/94‑1 (MQ=60)

                 CGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTT                                                                              >  SRR4302533.5473053/1‑94 (MQ=60)

                 CGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTC                                                                              >  SRR4302533.4402860/1‑94 (MQ=60)

                     CGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATA                                                                          >  SRR4302533.4232531/1‑94 (MQ=60)

                      GCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAA                                                                         >  SRR4302533.4017537/1‑94 (MQ=60)

                      GCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAA                                                                         >  SRR4302533.5288151/1‑94 (MQ=60)

                        GGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACC                                                                       <  SRR4302533.1625573/94‑1 (MQ=60)

                         GATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCC                                                                      >  SRR4302533.5817247/1‑94 (MQ=60)

                         GATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCC                                                                      >  SRR4302533.1285033/1‑94 (MQ=60)

                          ATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCA                                                                     <  SRR4302533.1374009/94‑1 (MQ=60)

                          ATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCA                                                                     >  SRR4302533.1243803/1‑94 (MQ=60)

                           TTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCAC                                                                    >  SRR4302533.3978330/1‑94 (MQ=60)

                           TTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCAC                                                                    <  SRR4302533.1598242/94‑1 (MQ=60)

                            ttttgtctgggtagggTCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACT                                                                   <  SRR4302533.3178450/78‑1 (MQ=60)

                            TTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACT                                                                   >  SRR4302533.5077543/1‑94 (MQ=60)

                            TTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACT                                                                   <  SRR4302533.6302145/94‑1 (MQ=60)

                             TTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACGGGCCCTGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTG                                                                  <  SRR4302533.3156683/94‑1 (MQ=60)

                             TTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTG                                                                  >  SRR4302533.683031/1‑94 (MQ=60)

                               GTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGT                                                                <  SRR4302533.5452213/94‑1 (MQ=60)

                                TGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTA                                                               >  SRR4302533.1369504/1‑94 (MQ=60)

                                 GCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTAC                                                              <  SRR4302533.3802736/94‑1 (MQ=60)

                                 GCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTAC                                                              <  SRR4302533.979132/94‑1 (MQ=60)

                                  CTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACG                                                             >  SRR4302533.3346247/1‑94 (MQ=60)

                                   TGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGT                                                            >  SRR4302533.1233302/1‑94 (MQ=60)

                                    GGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTC                                                           <  SRR4302533.769817/94‑1 (MQ=60)

                                    GGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTC                                                           <  SRR4302533.157438/94‑1 (MQ=60)

                                    GGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTC                                                           >  SRR4302533.5706015/1‑94 (MQ=60)

                                    GGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTC                                                           <  SRR4302533.5638172/94‑1 (MQ=60)

                                    GGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTC                                                           <  SRR4302533.2296357/94‑1 (MQ=60)

                                    GGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTC                                                           <  SRR4302533.3027542/94‑1 (MQ=60)

                                    GGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTC                                                           <  SRR4302533.2614508/94‑1 (MQ=60)

                                      TCAGGATCTTAACGATCGTAACGTTGATCCCACAGGACCCGCAGCGGATTTAGGGTTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGC                                                         >  SRR4302533.5711071/1‑94 (MQ=60)

                                       CAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCC                                                        >  SRR4302533.2298890/1‑94 (MQ=60)

                                        AGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCT                                                       <  SRR4302533.4003044/94‑1 (MQ=60)

                                         GGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTG                                                      <  SRR4302533.1049674/94‑1 (MQ=60)

                                         GGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTG                                                      <  SRR4302533.92495/94‑1 (MQ=60)

                                          GATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGG                                                     >  SRR4302533.1641317/1‑94 (MQ=60)

                                          GATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGG                                                     <  SRR4302533.4662024/94‑1 (MQ=60)

                                           ATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGC                                                    >  SRR4302533.1445982/1‑94 (MQ=60)

                                           ATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGC                                                    >  SRR4302533.40956/1‑94 (MQ=60)

                                           ATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGC                                                    <  SRR4302533.2821676/94‑1 (MQ=60)

                                             CTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAA                                                  >  SRR4302533.4506969/1‑94 (MQ=60)

                                               TAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAA                                                >  SRR4302533.3075426/1‑94 (MQ=60)

                                                AACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAAC                                               <  SRR4302533.4492083/94‑1 (MQ=60)

                                                AACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAAC                                               <  SRR4302533.935102/94‑1 (MQ=60)

                                                  CGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTG                                             >  SRR4302533.3998175/1‑94 (MQ=60)

                                                   GATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGC                                            >  SRR4302533.3462555/1‑94 (MQ=60)

                                                      CGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAA                                         >  SRR4302533.5471043/1‑94 (MQ=60)

                                                       GTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCACA                                        >  SRR4302533.4449224/1‑94 (MQ=60)

                                                          ACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACC                                     <  SRR4302533.4763883/94‑1 (MQ=60)

                                                           CGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCG                                    >  SRR4302533.1953971/1‑94 (MQ=60)

                                                           CGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCG                                    >  SRR4302533.967873/1‑94 (MQ=60)

                                                            GTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGG                                   >  SRR4302533.1213938/1‑94 (MQ=60)

                                                            GTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGG                                   >  SRR4302533.1056048/1‑94 (MQ=60)

                                                                ATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGAT                               >  SRR4302533.4733770/1‑94 (MQ=60)

                                                                ATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGAT                               <  SRR4302533.884063/94‑1 (MQ=60)

                                                                 TCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATC                              <  SRR4302533.5993938/94‑1 (MQ=60)

                                                                  CCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCT                             <  SRR4302533.4639799/94‑1 (MQ=60)

                                                                   CCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTG                            >  SRR4302533.2942485/1‑94 (MQ=60)

                                                                   CCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTG                            <  SRR4302533.5084676/94‑1 (MQ=60)

                                                                    CACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGT                           <  SRR4302533.494774/94‑1 (MQ=60)

                                                                    CACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGT                           <  SRR4302533.4865149/94‑1 (MQ=60)

                                                                     ACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGGGATCTGTG                          >  SRR4302533.3623185/1‑94 (MQ=60)

                                                                       AGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTAGCAAAACTGCCAAAACCGGTGATCTGTGCA                        <  SRR4302533.5832569/94‑1 (MQ=60)

                                                                        GGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAG                       <  SRR4302533.4398370/94‑1 (MQ=60)

                                                                         GCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGT                      >  SRR4302533.4503257/1‑94 (MQ=60)

                                                                           CCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCA                    >  SRR4302533.2173580/1‑94 (MQ=60)

                                                                              GCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATG                 <  SRR4302533.2163896/94‑1 (MQ=60)

                                                                              GCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATG                 <  SRR4302533.5777359/94‑1 (MQ=60)

                                                                               CACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGG                <  SRR4302533.4421294/94‑1 (MQ=60)

                                                                                 CCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCG              <  SRR4302533.4895754/94‑1 (MQ=60)

                                                                                  CGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGT             <  SRR4302533.3206953/94‑1 (MQ=60)

                                                                                  CGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGT             >  SRR4302533.2911971/1‑94 (MQ=60)

                                                                                  CGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGT             >  SRR4302533.4089885/1‑94 (MQ=60)

                                                                                   GGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTG            <  SRR4302533.708864/94‑1 (MQ=60)

                                                                                   GGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTG            <  SRR4302533.4842517/94‑1 (MQ=60)

                                                                                   GGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTG            <  SRR4302533.3798420/94‑1 (MQ=60)

                                                                                   GGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTG            <  SRR4302533.3134439/94‑1 (MQ=60)

                                                                                      TTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCG         >  SRR4302533.1201335/1‑94 (MQ=60)

                                                                                       TTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGG        >  SRR4302533.5375151/1‑94 (MQ=60)

                                                                                          GGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAG     <  SRR4302533.3427908/94‑1 (MQ=60)

                                                                                           GGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGG    >  SRR4302533.3033598/1‑94 (MQ=60)

                                                                                            GGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGG   >  SRR4302533.1638178/1‑94 (MQ=60)

                                                                                             GTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGG  <  SRR4302533.3561005/94‑1 (MQ=60)

                                                                                             GTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGG  <  SRR4302533.3289017/94‑1 (MQ=60)

CTGTTACTTACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGG  >  NC_000913/1456438‑1456624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`