Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,458,752 T→C R86R (CGT→CGC)  b1396 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,458,7520TC100.0% 775.0 / NA 245R86R (CGT→CGC) b1396hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (132/113);  total (132/113)

Only 100 of 252 total aligned reads displayed.

ATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGTCCAGGCTTTGCCGGAGACACCTTAACTGCTACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTTACGACATCGAAATTGTTAA  >  NC_000913/1458662‑1458841
                                                                                          |                                                                                         
aTTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGccc                                                                                         >  1:2032971/1‑93 (MQ=255)
 ttttCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCa                                                                                        >  2:1251275/1‑93 (MQ=255)
      aCTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCtt                                                                                   >  2:1756902/1‑93 (MQ=255)
      aCTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCtt                                                                                   >  1:2819804/1‑93 (MQ=255)
       cTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCttt                                                                                  <  2:1936352/93‑1 (MQ=255)
        tGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTg                                                                                 <  1:3781737/93‑1 (MQ=255)
        tGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTg                                                                                 <  1:2385/93‑1 (MQ=255)
         ggCTGATCCTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGc                                                                                <  2:5280142/93‑1 (MQ=255)
         ggCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGc                                                                                <  2:4982774/93‑1 (MQ=255)
         ggCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGc                                                                                >  1:838311/1‑93 (MQ=255)
          gCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGcc                                                                               >  2:669905/1‑93 (MQ=255)
             gATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGga                                                                            >  1:2660085/1‑93 (MQ=255)
             gATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGga                                                                            >  1:5613957/1‑93 (MQ=255)
               tACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGgaga                                                                          <  2:2102779/93‑1 (MQ=255)
                aCTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGac                                                                         >  1:4604222/1‑93 (MQ=255)
                  tGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGacac                                                                       <  2:5749218/93‑1 (MQ=255)
                   gCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACAcc                                                                      >  2:43516/1‑93 (MQ=255)
                   gCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACAcc                                                                      >  1:4409403/1‑93 (MQ=255)
                    ccTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCt                                                                     <  1:3641203/93‑1 (MQ=255)
                      tttGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTa                                                                   >  1:5927964/1‑93 (MQ=255)
                       ttGCCTACGCCTGCAATAGCCAGGTGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTaa                                                                  <  2:5837756/93‑1 (MQ=255)
                        tGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAAc                                                                 <  2:2601751/93‑1 (MQ=255)
                         gCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACt                                                                <  2:2970899/93‑1 (MQ=255)
                           cTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACAGCTTAACTGc                                                              >  1:1314401/1‑93 (MQ=255)
                             aCGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGcca                                                            <  2:5145644/93‑3 (MQ=255)
                             aCGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGACGGAGACACCTTAACTGcca                                                            >  1:4526200/1‑91 (MQ=255)
                              cGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCAc                                                           <  2:3540088/93‑1 (MQ=255)
                              cGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCAc                                                           >  2:2020329/1‑93 (MQ=255)
                               gCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACt                                                          <  1:6263025/93‑1 (MQ=255)
                               gCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACt                                                          <  1:4285664/93‑1 (MQ=255)
                               gCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACt                                                          >  2:5606102/1‑93 (MQ=255)
                                ccTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTg                                                         <  1:5195566/93‑1 (MQ=255)
                                ccTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTg                                                         <  2:1715244/93‑1 (MQ=255)
                                ccTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTg                                                         >  2:3703636/1‑93 (MQ=255)
                                 cTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTgc                                                        >  1:5549013/1‑93 (MQ=255)
                                 cTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTgc                                                        >  1:5694400/1‑93 (MQ=255)
                                 cTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTgc                                                        <  1:6058817/93‑1 (MQ=255)
                                  tGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTgcg                                                       >  2:2214017/1‑93 (MQ=255)
                                  tGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTgcg                                                       >  2:2169120/1‑93 (MQ=255)
                                  tGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTgcg                                                       <  2:3506701/93‑1 (MQ=255)
                                    cAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGTGACACCTTAACTGCCACTGCGCa                                                     >  2:2566824/1‑93 (MQ=255)
                                    cAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCa                                                     <  2:3157291/93‑1 (MQ=255)
                                    cAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCa                                                     <  1:2761890/93‑1 (MQ=255)
                                     aaTAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAg                                                    <  2:781863/93‑1 (MQ=255)
                                      aTAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAgg                                                   <  1:1495497/93‑1 (MQ=255)
                                      aTAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAgg                                                   >  1:4933155/1‑93 (MQ=255)
                                       tAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGt                                                  >  2:840916/1‑93 (MQ=255)
                                         gCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTAc                                                >  2:2352346/1‑93 (MQ=255)
                                          ccAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACg                                               >  1:3043029/1‑93 (MQ=255)
                                            aGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGtc                                             >  1:1274312/1‑93 (MQ=255)
                                             ggggCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGtca                                            <  2:5430779/93‑1 (MQ=255)
                                             ggggCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGtca                                            <  1:5752036/93‑1 (MQ=255)
                                               ggCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGtcatc                                          <  1:1902862/93‑1 (MQ=255)
                                                gCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGtcatca                                         <  2:1612798/93‑1 (MQ=255)
                                                gCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGtcatca                                         >  2:4568595/1‑93 (MQ=255)
                                                 cTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAg                                        <  1:2620561/93‑1 (MQ=255)
                                                  ttgCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAgg                                       <  2:3907551/91‑1 (MQ=255)
                                                  tGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAgg                                       <  1:157751/93‑1 (MQ=255)
                                                   ggCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAggg                                      <  2:4409403/93‑1 (MQ=255)
                                                   ggCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAggg                                      >  1:4085039/1‑93 (MQ=255)
                                                    gCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGgc                                     >  2:3240133/1‑93 (MQ=255)
                                                    gCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGgc                                     <  1:5998406/93‑1 (MQ=255)
                                                      aGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGgcaa                                   <  2:2915881/93‑1 (MQ=255)
                                                       gCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGgcaag                                  <  1:991086/93‑1 (MQ=255)
                                                        ccGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGgcaagc                                 <  2:2764449/93‑1 (MQ=255)
                                                        ccGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGgcaagc                                 <  2:1825411/93‑1 (MQ=255)
                                                         cGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGgcaagca                                >  1:4693818/1‑93 (MQ=255)
                                                           tCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCGAGCaaa                              >  1:3054196/1‑93 (MQ=255)
                                                           tCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCaaa                              >  1:2507692/1‑93 (MQ=255)
                                                            cGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAAc                             <  2:747231/93‑1 (MQ=255)
                                                            cGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAAc                             <  2:4118658/93‑1 (MQ=255)
                                                             gCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAAcc                            >  1:5152278/1‑93 (MQ=255)
                                                              cTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCg                           <  2:4279009/93‑1 (MQ=255)
                                                               ttCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCgg                          >  1:4796363/1‑93 (MQ=255)
                                                               ttCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCgg                          >  1:3424140/1‑93 (MQ=255)
                                                               ttCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCgg                          <  2:4210711/93‑1 (MQ=255)
                                                                 ctgcctgcACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGgtg                        >  1:1036368/1‑93 (MQ=255)
                                                                 ctgcctgcACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGgtg                        <  2:4844727/93‑1 (MQ=255)
                                                                  tgcctgcACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGgtgt                       >  2:1355623/1‑93 (MQ=255)
                                                                   gcctgcACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGtt                      <  2:1152024/93‑1 (MQ=255)
                                                                   gcctgcACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGtt                      <  2:579973/93‑1 (MQ=255)
                                                                    cctgcACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGttt                     >  2:5598216/1‑93 (MQ=255)
                                                                    cctgcACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGttt                     >  1:6149576/1‑93 (MQ=255)
                                                                     ctgcACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGtttc                    >  1:395733/1‑92 (MQ=255)
                                                                     ctgcACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTTa                    >  1:2061789/1‑93 (MQ=255)
                                                                      tgcACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTTAc                   >  2:5935780/1‑93 (MQ=255)
                                                                      tgcACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTTAc                   <  2:3665240/93‑1 (MQ=255)
                                                                       gcACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTTACg                  >  2:2740669/1‑93 (MQ=255)
                                                                         aCGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTTACGAc                >  2:5351707/1‑93 (MQ=255)
                                                                         aCGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTTACGAc                <  2:5501252/93‑1 (MQ=255)
                                                                           gattgattTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTTACGACAt              >  1:4006647/1‑93 (MQ=255)
                                                                           gattgattTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTTACGACAt              <  2:485125/93‑1 (MQ=255)
                                                                            attgattTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGTAAACCGGTGCTTACGACATc             >  1:6304669/1‑93 (MQ=255)
                                                                            attgattTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTTACGACATc             <  1:5319763/93‑1 (MQ=255)
                                                                              tgattTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTTACGACATCGa           <  2:624131/93‑1 (MQ=255)
                                                                                 ttttttGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTTACGACATCGAAAt        <  2:4523149/93‑1 (MQ=255)
                                                                                  tttttGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTTACGACATCGAAAtt       >  1:3175673/1‑93 (MQ=255)
                                                                                    tttGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGCTTACGACATCGAAATTGt     <  2:3770586/93‑1 (MQ=255)
                                                                                      tGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTTACGACATCGAAATTGTTa   <  2:1472504/93‑1 (MQ=255)
                                                                                       gcgcCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTTACGACATCGAAATTGTTaa  <  2:2425459/93‑1 (MQ=255)
                                                                                          |                                                                                         
ATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGTCCAGGCTTTGCCGGAGACACCTTAACTGCTACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTTACGACATCGAAATTGTTAA  >  NC_000913/1458662‑1458841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGTCCAGGCTTTGCCGGAGACACCTTAACTGCTACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTTACGACATCGAAATTGTTAACCAA  >  NC_000913/1458659‑1458845
                                                                                             |                                                                                             
GCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGC                                                                                               >  SRR4302533.57234/1‑94 (MQ=60)
 CTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCC                                                                                              >  SRR4302533.4458966/1‑94 (MQ=60)
  TATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCC                                                                                             <  SRR4302533.1347193/94‑1 (MQ=60)
  TATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCC                                                                                             >  SRR4302533.4007995/1‑94 (MQ=60)
  TATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCC                                                                                             >  SRR4302533.2045515/1‑94 (MQ=60)
   ATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCA                                                                                            >  SRR4302533.4550529/1‑94 (MQ=60)
       TCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCT                                                                                        >  SRR4302533.4617393/1‑94 (MQ=60)
        CACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTT                                                                                       >  SRR4302533.2837156/1‑94 (MQ=60)
        CACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTT                                                                                       >  SRR4302533.3669831/1‑94 (MQ=60)
           TGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGC                                                                                    >  SRR4302533.843220/1‑94 (MQ=60)
           TGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGC                                                                                    <  SRR4302533.3804900/94‑1 (MQ=60)
           TGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGC                                                                                    <  SRR4302533.2403/94‑1 (MQ=60)
              CTGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGG                                                                                 >  SRR4302533.1731215/1‑94 (MQ=60)
               TGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGA                                                                                <  SRR4302533.2264517/94‑1 (MQ=60)
               TGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGA                                                                                >  SRR4302533.5647889/1‑94 (MQ=60)
               TGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGA                                                                                >  SRR4302533.2676420/1‑94 (MQ=60)
                GATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAG                                                                               >  SRR4302533.2508262/1‑94 (MQ=60)
                 ATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGA                                                                              <  SRR4302533.4419347/94‑1 (MQ=60)
                  TACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGAC                                                                             >  SRR4302533.4632115/1‑94 (MQ=60)
                   ACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACA                                                                            <  SRR4302533.2304658/94‑1 (MQ=60)
                     TGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACC                                                                          >  SRR4302533.4436030/1‑94 (MQ=60)
                      GCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCT                                                                         >  SRR4302533.1283025/1‑94 (MQ=60)
                      GCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCT                                                                         <  SRR4302533.4873509/94‑1 (MQ=60)
                       CCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTT                                                                        <  SRR4302533.416976/94‑1 (MQ=60)
                       CCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTT                                                                        <  SRR4302533.3663596/94‑1 (MQ=60)
                        CTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTA                                                                       >  SRR4302533.5963798/1‑94 (MQ=60)
                        CTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTA                                                                       >  SRR4302533.1308840/1‑94 (MQ=60)
                         TTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAA                                                                      <  SRR4302533.5058873/94‑1 (MQ=60)
                          TTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAAC                                                                     <  SRR4302533.5629281/94‑1 (MQ=60)
                           TGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACT                                                                    >  SRR4302533.598564/1‑94 (MQ=60)
                            GCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTG                                                                   >  SRR4302533.5262771/1‑94 (MQ=60)
                             CCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACAGCTTAACTGC                                                                  >  SRR4302533.1322287/1‑94 (MQ=60)
                             CCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGC                                                                  >  SRR4302533.4421162/1‑94 (MQ=60)
                             CCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGC                                                                  >  SRR4302533.6283972/1‑94 (MQ=60)
                              CTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGTACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCC                                                                 >  SRR4302533.6334293/1‑94 (MQ=60)
                              CTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCC                                                                 >  SRR4302533.4080634/1‑94 (MQ=60)
                              CTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCC                                                                 >  SRR4302533.150885/1‑94 (MQ=60)
                              CTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCC                                                                 >  SRR4302533.3165398/1‑94 (MQ=60)
                               TACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGACGGAGACACCTTAACTGCCA                                                                >  SRR4302533.4553602/1‑94 (MQ=60)
                                  GCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTG                                                             <  SRR4302533.905018/94‑1 (MQ=60)
                                  GCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTG                                                             <  SRR4302533.6300461/94‑1 (MQ=60)
                                  GCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTG                                                             <  SRR4302533.4311413/94‑1 (MQ=60)
                                   CCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGC                                                            >  SRR4302533.5728789/1‑94 (MQ=60)
                                   CCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGC                                                            <  SRR4302533.5226901/94‑1 (MQ=60)
                                   CCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGC                                                            >  SRR4302533.5582521/1‑94 (MQ=60)
                                    CTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCG                                                           <  SRR4302533.6095497/94‑1 (MQ=60)
                                    CTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCG                                                           >  SRR4302533.4063700/1‑94 (MQ=60)
                                    CTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCG                                                           >  SRR4302533.4688090/1‑94 (MQ=60)
                                       CAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAG                                                        <  SRR4302533.6238205/94‑1 (MQ=60)
                                       CAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAG                                                        <  SRR4302533.2778823/94‑1 (MQ=60)
                                       CAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAG                                                        <  SRR4302533.6073254/94‑1 (MQ=60)
                                        AATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGG                                                       >  SRR4302533.4963026/1‑94 (MQ=60)
                                         ATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGT                                                      <  SRR4302533.1504508/94‑1 (MQ=60)
                                          TAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTA                                                     <  SRR4302533.2667416/94‑1 (MQ=60)
                                          TAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTA                                                     <  SRR4302533.3868310/94‑1 (MQ=60)
                                          TAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTA                                                     >  SRR4302533.4883290/1‑94 (MQ=60)
                                           AGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTAC                                                    <  SRR4302533.2070480/94‑1 (MQ=60)
                                           AGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTAC                                                    >  SRR4302533.3354515/1‑94 (MQ=60)
                                           AGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTAC                                                    <  SRR4302533.5478582/94‑1 (MQ=60)
                                            GCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACG                                                   >  SRR4302533.2778216/1‑94 (MQ=60)
                                            GCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACG                                                   >  SRR4302533.3061658/1‑94 (MQ=60)
                                              CAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTC                                                 >  SRR4302533.589861/1‑94 (MQ=60)
                                              CAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTC                                                 >  SRR4302533.84082/1‑94 (MQ=60)
                                              CAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTC                                                 >  SRR4302533.1281971/1‑94 (MQ=60)
                                                GGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCAT                                               <  SRR4302533.5786737/94‑1 (MQ=60)
                                                 GGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATC                                              >  SRR4302533.1273431/1‑94 (MQ=60)
                                                 GGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATC                                              >  SRR4302533.6038453/1‑94 (MQ=60)
                                                  GGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCA                                             <  SRR4302533.1914475/94‑1 (MQ=60)
                                                    CTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGG                                           <  SRR4302533.2636646/94‑1 (MQ=60)
                                                     TGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGG                                          >  SRR4302533.4109907/1‑94 (MQ=60)
                                                     TGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGG                                          <  SRR4302533.158757/94‑1 (MQ=60)
                                                       GCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCA                                        <  SRR4302533.6034737/94‑1 (MQ=60)
                                                         AGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAG                                      >  SRR4302533.1546972/1‑94 (MQ=60)
                                                         AGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAG                                      >  SRR4302533.1970910/1‑94 (MQ=60)
                                                          GCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGC                                     <  SRR4302533.996882/94‑1 (MQ=60)
                                                          GCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGC                                     <  SRR4302533.2770379/94‑1 (MQ=60)
                                                          GCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGC                                     <  SRR4302533.315880/94‑1 (MQ=60)
                                                           CCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCA                                    >  SRR4302533.4722262/1‑94 (MQ=60)
                                                             GTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCGAGCAAA                                  >  SRR4302533.3072911/1‑94 (MQ=60)
                                                             GTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAA                                  >  SRR4302533.2523147/1‑94 (MQ=60)
                                                             GTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAA                                  >  SRR4302533.5726936/1‑94 (MQ=60)
                                                             GTCGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAA                                  >  SRR4302533.1986250/1‑94 (MQ=60)
                                                               CGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACC                                >  SRR4302533.3062727/1‑94 (MQ=60)
                                                               CGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACC                                <  SRR4302533.4797132/94‑1 (MQ=60)
                                                               CGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACC                                >  SRR4302533.5183402/1‑94 (MQ=60)
                                                               CGCTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACC                                >  SRR4302533.5183401/1‑94 (MQ=60)
                                                                 CTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGG                              >  SRR4302533.3445292/1‑94 (MQ=60)
                                                                 CTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGG                              >  SRR4302533.4825417/1‑94 (MQ=60)
                                                                 CTTCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGG                              >  SRR4302533.2620942/1‑94 (MQ=60)
                                                                   TCTGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTG                            >  SRR4302533.1042460/1‑94 (MQ=60)
                                                                     TGCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTT                          >  SRR4302533.5221260/1‑94 (MQ=60)
                                                                     TGCCTGCACGATTGATTTTTTGAGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTT                          >  SRR4302533.5686096/1‑94 (MQ=60)
                                                                      GCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTT                         >  SRR4302533.5759444/1‑94 (MQ=60)
                                                                      GCCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTT                         >  SRR4302533.6186593/1‑94 (MQ=60)
                                                                       CCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTTC                        >  SRR4302533.398131/1‑94 (MQ=60)
                                                                       CCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTTA                        >  SRR4302533.2074503/1‑94 (MQ=60)
                                                                       CCTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGAGTTTA                        >  SRR4302533.2882894/1‑94 (MQ=60)
                                                                        CTGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTTAC                       <  SRR4302533.5848379/94‑1 (MQ=60)
                                                                         TGCACGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTTACG                      <  SRR4302533.1471503/94‑1 (MQ=60)
                                                                             CGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTTACGACAT                  <  SRR4302533.3620280/94‑1 (MQ=60)
                                                                             CGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTTACGACAT                  >  SRR4302533.4467641/1‑94 (MQ=60)
                                                                             CGATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTTACGACAT                  >  SRR4302533.4031040/1‑94 (MQ=60)
                                                                              GATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGTAAACCGGTGCTTACGACATC                 >  SRR4302533.6342384/1‑94 (MQ=60)
                                                                              GATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTTACGACATC                 <  SRR4302533.2948071/94‑1 (MQ=60)
                                                                              GATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTTACGACATC                 <  SRR4302533.4674008/94‑1 (MQ=60)
                                                                               ATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTTACGACATCG                >  SRR4302533.824238/1‑94 (MQ=60)
                                                                               ATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTTACGACATCG                <  SRR4302533.5351646/94‑1 (MQ=60)
                                                                               ATTGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTTACGACATCG                >  SRR4302533.3590208/1‑94 (MQ=60)
                                                                                 TGATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTTACGACATCGAA              <  SRR4302533.2536817/94‑1 (MQ=60)
                                                                                  GATTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTTACGACATCGAAA             <  SRR4302533.6171403/94‑1 (MQ=60)
                                                                                    TTTTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTTACGACATCGAAATT           >  SRR4302533.3195209/1‑94 (MQ=60)
                                                                                      TTTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTTACGACATCGAAATTGT         >  SRR4302533.5453397/1‑94 (MQ=60)
                                                                                       TTTGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTTACGACATCGAAATTGTT        <  SRR4302533.2795905/94‑1 (MQ=60)
                                                                                         TGCGCCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTTACGACATCGAAATTGTTAA      <  SRR4302533.3820785/94‑1 (MQ=60)
                                                                                             CCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTTACGACATCGAAATTGTTAACCAA  >  SRR4302533.4825053/1‑94 (MQ=60)
                                                                                             CCCAGGCTTTGCCGGAGACACCTTAACTGCCACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTTACGACATCGAAATTGTTAACCAA  <  SRR4302533.3366976/94‑1 (MQ=60)
                                                                                             |                                                                                             
GCTATTTTCACTGGCTGATACTGCCTTTGCCTACGCCTGCAATAGCCAGGGGCTGGCAGCCGTCGCTTCTGCCTGCACGATTGATTTTTTGCGTCCAGGCTTTGCCGGAGACACCTTAACTGCTACTGCGCAGGTACGTCATCAGGGCAAGCAAACCGGTGTTTACGACATCGAAATTGTTAACCAA  >  NC_000913/1458659‑1458845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑