Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,481,375	C→T	I97I (ATC→ATT)	hrpA →	helicase, ATP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,481,375	0	C	T	100.0%	835.2 / NA	222	I97I (ATC→ATT)	hrpA	helicase, ATP‑dependent
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (115/107); total (115/107)

Only 100 of 232 total aligned reads displayed.

ACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCA  >  NC_000913/1481285‑1481464

gcGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGAttgt                                                                                         >  1:4395064/2‑93 (MQ=255)

 cGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGAttgtc                                                                                        >  1:2495375/1‑93 (MQ=255)

 cGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGAttgtc                                                                                        <  1:47430/93‑1 (MQ=255)

    ccGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGcc                                                                                     <  2:4141751/93‑1 (MQ=255)

    ccGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGcc                                                                                     <  1:2592454/93‑1 (MQ=255)

        aaaTTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCgggg                                                                                 >  1:1398567/1‑93 (MQ=255)

        aaaTTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCgggg                                                                                 <  1:442531/93‑1 (MQ=255)

        aaaTTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCgggg                                                                                 <  1:3428751/93‑1 (MQ=255)

        aaaTTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCgggg                                                                                 <  1:768888/93‑1 (MQ=255)

        aaaTTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCgggg                                                                                 >  1:1735219/1‑93 (MQ=255)

         aaTTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGa                                                                                >  1:1079547/1‑93 (MQ=255)

          aTTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGaa                                                                               >  2:2459339/1‑93 (MQ=255)

            tACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAAc                                                                             <  1:1214240/93‑1 (MQ=255)

            tACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAAc                                                                             <  1:1961366/93‑1 (MQ=255)

             aCTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACg                                                                            >  2:5603134/1‑93 (MQ=255)

              cTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACgg                                                                           <  2:4806014/93‑1 (MQ=255)

              cTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACgg                                                                           <  1:2278686/93‑1 (MQ=255)

              cTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACgg                                                                           >  2:800368/1‑93 (MQ=255)

              cTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACgg                                                                           <  2:6208640/93‑1 (MQ=255)

               ttATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACggg                                                                          <  1:2442759/93‑1 (MQ=255)

                tATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGt                                                                         >  1:3268195/1‑93 (MQ=255)

                tATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGt                                                                         <  1:35654/93‑1 (MQ=255)

                 aTCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGtt                                                                        <  2:4570232/93‑1 (MQ=255)

                 aTCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGtt                                                                        <  2:1570716/93‑1 (MQ=255)

                    cTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTg                                                                     <  2:5846499/93‑1 (MQ=255)

                      gACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGt                                                                   >  2:4489873/1‑93 (MQ=255)

                      gACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGt                                                                   <  1:4909453/93‑1 (MQ=255)

                         aaTTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAATCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTaaa                                                                <  2:4823892/93‑1 (MQ=255)

                         aaTTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGGTCTGGTaaa                                                                >  1:1256582/1‑93 (MQ=255)

                          aTTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTaaaa                                                               <  2:1764635/93‑1 (MQ=255)

                          aTTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTaaaa                                                               >  2:1459532/1‑93 (MQ=255)

                           tttACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAAc                                                              >  2:5634603/1‑93 (MQ=255)

                             tACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGa                                                            <  2:4389571/93‑1 (MQ=255)

                             tACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGa                                                            >  2:4714520/1‑93 (MQ=255)

                             tACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGa                                                            >  2:1609619/1‑93 (MQ=255)

                             tACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGGGATTGTCGCCGGGGAAACGGGTTCTGGTAATACGa                                                            >  2:130240/1‑93 (MQ=255)

                               ccGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACt                                                          >  1:6277784/1‑93 (MQ=255)

                                cGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTc                                                         <  2:1653459/93‑1 (MQ=255)

                                cGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTc                                                         >  1:6093182/1‑93 (MQ=255)

                                 ggTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCTGGGGAAACGGGGTCTGGTAAAACGACTCa                                                        >  2:4344841/1‑93 (MQ=255)

                                 ggTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCa                                                        <  1:276784/93‑1 (MQ=255)

                                 ggTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCa                                                        >  2:2910708/1‑93 (MQ=255)

                                  gTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAg                                                       >  2:2172408/1‑93 (MQ=255)

                                    tAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGtt                                                     <  2:2510834/93‑1 (MQ=255)

                                      gTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTAc                                                   <  1:3247910/93‑1 (MQ=255)

                                      gTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTAc                                                   <  2:2896050/93‑1 (MQ=255)

                                      gTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTAc                                                   >  1:616481/1‑93 (MQ=255)

                                         aGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGa                                                >  2:28365/1‑93 (MQ=255)

                                         aGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGa                                                <  1:420754/93‑1 (MQ=255)

                                          gAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGaa                                               >  1:5864987/1‑93 (MQ=255)

                                          gAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGaa                                               >  1:4948318/1‑93 (MQ=255)

                                             aaaaaaAGACGTTCACGAAGCGTTTTGGTGATCCCGGGGTGGTTTTTGCCGCGGGGGAAACGGTTTCTGGTAAAACGACTCAGTTCCCGaaaa                                             <  2:6079603/93‑1 (MQ=255)

                                              aaaCAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATc                                           >  1:898824/1‑93 (MQ=255)

                                              aaaCAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATc                                           <  1:5742501/93‑1 (MQ=255)

                                              aaaCAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATc                                           >  1:5673279/1‑93 (MQ=255)

                                              aaaCAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATc                                           <  2:2056017/93‑1 (MQ=255)

                                               aaCAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCt                                          >  2:3540723/1‑93 (MQ=255)

                                                aCAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCACTTACCGaaaacccg                                         >  2:1162277/1‑89 (MQ=255)

                                                  aGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTa                                       <  2:1796301/93‑1 (MQ=255)

                                                  aGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTa                                       >  1:1547686/1‑93 (MQ=255)

                                                  aGGACATTCGCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTa                                       <  2:2371632/93‑1 (MQ=255)

                                                   ggACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTAt                                      >  2:3051028/1‑93 (MQ=255)

                                                   ggACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTAt                                      <  1:4592664/93‑1 (MQ=255)

                                                   ggACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTAt                                      >  1:1859276/1‑93 (MQ=255)

                                                   ggACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAATTACCGAAAATCTGTAt                                      >  1:1813031/1‑93 (MQ=255)

                                                     aCATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATgg                                    <  1:2777195/93‑1 (MQ=255)

                                                     aCATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATgg                                    <  2:5358381/93‑1 (MQ=255)

                                                     aCATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATgg                                    <  1:5236444/93‑1 (MQ=255)

                                                      cATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGa                                   >  1:5451209/1‑93 (MQ=255)

                                                      cATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGa                                   >  2:2395987/1‑93 (MQ=255)

                                                        ttCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGc                                 >  1:3150195/1‑93 (MQ=255)

                                                          ctcGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTg                               <  2:6186386/93‑1 (MQ=255)

                                                           tcGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTgg                              >  2:768744/1‑93 (MQ=255)

                                                           tcGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTgg                              >  2:5352027/1‑93 (MQ=255)

                                                            cGAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTggg                             >  1:3802815/1‑93 (MQ=255)

                                                             gAAGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGGTCTGGTAAAACGACTCAGTTACCGCAAATCTGTATGGAGCTgggg                            >  1:2694788/1‑93 (MQ=255)

                                                              aaGCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGgc                           >  2:6262437/1‑93 (MQ=255)

                                                                gCG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGgcgc                         <  2:938506/93‑1 (MQ=255)

                                                                 cG‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGcgcg                        <  1:3579508/93‑1 (MQ=255)

                                                                  g‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCgg                       <  2:3794364/93‑1 (MQ=255)

                                                                  g‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCgg                       >  2:2094109/1‑93 (MQ=255)

                                                                  g‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCgg                       <  2:258294/93‑1 (MQ=255)

                                                                  g‑ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCgg                       >  2:3971077/1‑93 (MQ=255)

                                                                    aTTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCggg                      >  2:3315728/1‑93 (MQ=255)

                                                                     ttCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGa                     >  1:2034661/1‑93 (MQ=255)

                                                                      tCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGAt                    <  1:147167/93‑1 (MQ=255)

                                                                      tCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGGTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGAt                    >  1:4035605/1‑93 (MQ=255)

                                                                        ctGATCACCAGGTGGTGATTGTCGCCCGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTa                  <  2:3126339/92‑1 (MQ=255)

                                                                         tGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTaa                 >  2:4906103/1‑93 (MQ=255)

                                                                         tGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTaa                 <  2:1482028/93‑1 (MQ=255)

                                                                           aTCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAg               >  1:2527251/1‑93 (MQ=255)

                                                                            tCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAgg              >  1:5700272/1‑93 (MQ=255)

                                                                             cACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGa             <  2:1952418/93‑1 (MQ=255)

                                                                              aCCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGAc            >  2:5100100/1‑93 (MQ=255)

                                                                              aCCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGAc            >  2:2311364/1‑93 (MQ=255)

                                                                              aCCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGAc            >  1:2846999/1‑93 (MQ=255)

                                                                                cAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTg          <  1:1233909/93‑1 (MQ=255)

                                                                                    tggtgATTGTCGCGGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCg      <  1:3998523/93‑1 (MQ=255)

                                                                                    tggtgATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCg      <  1:1460863/93‑1 (MQ=255)

                                                                                        gATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCa  >  1:5131069/1‑93 (MQ=255)

ACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG‑ATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCA  >  NC_000913/1481285‑1481464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATA  >  NC_000913/1481284‑1481466

CGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGT                                                                                           >  SRR4302533.4421599/1‑94 (MQ=60)

 GCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTC                                                                                          >  SRR4302533.2510768/1‑94 (MQ=60)

  CGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCG                                                                                         >  SRR4302533.2740054/1‑94 (MQ=60)

  CGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCG                                                                                         <  SRR4302533.47758/94‑1 (MQ=60)

   GACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGC                                                                                        >  SRR4302533.2184132/1‑94 (MQ=60)

     CCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCG                                                                                      <  SRR4302533.3268837/94‑1 (MQ=60)

     CCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCG                                                                                      <  SRR4302533.2608385/94‑1 (MQ=60)

       GGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGG                                                                                    >  SRR4302533.1315777/1‑94 (MQ=60)

       GGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGG                                                                                    <  SRR4302533.2590170/94‑1 (MQ=60)

        GAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGG                                                                                   >  SRR4302533.1406996/1‑94 (MQ=60)

        GAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGG                                                                                   >  SRR4302533.1745745/1‑94 (MQ=60)

         AAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGA                                                                                  >  SRR4302533.1085909/1‑94 (MQ=60)

         AAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGA                                                                                  <  SRR4302533.3449934/94‑1 (MQ=60)

         AAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGA                                                                                  <  SRR4302533.2844235/94‑1 (MQ=60)

         AAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGA                                                                                  >  SRR4302533.2752895/1‑94 (MQ=60)

         AAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGA                                                                                  <  SRR4302533.445177/94‑1 (MQ=60)

         AAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGA                                                                                  <  SRR4302533.773419/94‑1 (MQ=60)

            TTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAAC                                                                               <  SRR4302533.1847430/94‑1 (MQ=60)

            TTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAAC                                                                               <  SRR4302533.5927216/94‑1 (MQ=60)

             TACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACG                                                                              >  SRR4302533.712691/1‑94 (MQ=60)

             TACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACG                                                                              >  SRR4302533.1839646/1‑94 (MQ=60)

             TACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACG                                                                              <  SRR4302533.1221536/94‑1 (MQ=60)

             TACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACG                                                                              <  SRR4302533.1973339/94‑1 (MQ=60)

               CTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGG                                                                            <  SRR4302533.2292795/94‑1 (MQ=60)

                TTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGT                                                                           <  SRR4302533.2457838/94‑1 (MQ=60)

                TTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGT                                                                           >  SRR4302533.3288362/1‑94 (MQ=60)

                 TATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTT                                                                          <  SRR4302533.35893/94‑1 (MQ=60)

                 TATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTT                                                                          <  SRR4302533.3371007/94‑1 (MQ=60)

                    CCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTG                                                                       >  SRR4302533.3633141/1‑94 (MQ=60)

                    CCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTG                                                                       <  SRR4302533.2414110/94‑1 (MQ=60)

                     CTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGG                                                                      <  SRR4302533.5182787/94‑1 (MQ=60)

                     CTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGG                                                                      <  SRR4302533.3599110/94‑1 (MQ=60)

                       GCCAATTTACCGGTTAGTCATAAAAACCAGGCCATTCTCGAAGCTATTCGTGATCACCAGGTGGTGATTGTCGCCGGGTAAACGTGTTCTGGTA                                                                    <  SRR4302533.4939196/94‑1 (MQ=60)

                       GACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTA                                                                    <  SRR4302533.4939195/94‑1 (MQ=60)

                         CAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGGTCTGGTAAA                                                                  >  SRR4302533.1264094/1‑94 (MQ=60)

                          AATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAA                                                                 >  SRR4302533.4484213/1‑94 (MQ=60)

                           ATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAAC                                                                <  SRR4302533.4028034/94‑1 (MQ=60)

                             TTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGA                                                              >  SRR4302533.5108722/1‑94 (MQ=60)

                             TTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGA                                                              >  SRR4302533.5785250/1‑94 (MQ=60)

                               ACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACT                                                            >  SRR4302533.6315265/1‑94 (MQ=60)

                                CCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTC                                                           >  SRR4302533.6130001/1‑94 (MQ=60)

                                CCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGACTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTC                                                           >  SRR4302533.1371176/1‑94 (MQ=60)

                                  GGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAG                                                         <  SRR4302533.278469/94‑1 (MQ=60)

                                  GGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAG                                                         >  SRR4302533.4993348/1‑94 (MQ=60)

                                   GTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGT                                                        <  SRR4302533.121313/94‑1 (MQ=60)

                                   GTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGT                                                        <  SRR4302533.3173379/94‑1 (MQ=60)

                                      AGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTAC                                                     <  SRR4302533.3003689/94‑1 (MQ=60)

                                      AGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTAC                                                     <  SRR4302533.5689482/94‑1 (MQ=60)

                                      AGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTAC                                                     >  SRR4302533.620100/1‑94 (MQ=60)

                                       GTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACC                                                    <  SRR4302533.3267939/94‑1 (MQ=60)

                                        TCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCG                                                   <  SRR4302533.2645086/94‑1 (MQ=60)

                                         CAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGA                                                  >  SRR4302533.171981/1‑94 (MQ=60)

                                          AGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAA                                                 >  SRR4302533.5900445/1‑94 (MQ=60)

                                          AGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAA                                                 >  SRR4302533.4978279/1‑94 (MQ=60)

                                          AGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAA                                                 <  SRR4302533.423271/94‑1 (MQ=60)

                                            AAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATGGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAA                                               <  SRR4302533.2474515/94‑1 (MQ=60)

                                             AAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAAT                                              >  SRR4302533.1820814/1‑94 (MQ=60)

                                             AAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAAT                                              >  SRR4302533.5730794/1‑94 (MQ=60)

                                              AAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATC                                             >  SRR4302533.904097/1‑94 (MQ=60)

                                              AAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATC                                             >  SRR4302533.5707542/1‑94 (MQ=60)

                                               AAGCAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACCACTCAGTTACCGAAAATCT                                            <  SRR4302533.5743722/94‑1 (MQ=60)

                                               AAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCT                                            <  SRR4302533.5777151/94‑1 (MQ=60)

                                                AACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTG                                           <  SRR4302533.4368449/94‑1 (MQ=60)

                                                AACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTG                                           >  SRR4302533.2514874/1‑94 (MQ=60)

                                                AACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTG                                           >  SRR4302533.6165837/1‑94 (MQ=60)

                                                AACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTG                                           >  SRR4302533.3133278/1‑94 (MQ=60)

                                                 ACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGT                                          <  SRR4302533.5452470/94‑1 (MQ=60)

                                                  CAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTA                                         <  SRR4302533.2788401/94‑1 (MQ=60)

                                                  CAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTA                                         >  SRR4302533.1557012/1‑94 (MQ=60)

                                                  CAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTA                                         <  SRR4302533.6288373/94‑1 (MQ=60)

                                                   AGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTAT                                        >  SRR4302533.1870617/1‑94 (MQ=60)

                                                   AGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAATTACCGAAAATCTGTAT                                        >  SRR4302533.1824103/1‑94 (MQ=60)

                                                    GGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATG                                       <  SRR4302533.4620484/94‑1 (MQ=60)

                                                    GGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATG                                       <  SRR4302533.6298981/94‑1 (MQ=60)

                                                      ACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGA                                     <  SRR4302533.5267929/94‑1 (MQ=60)

                                                      ACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGA                                     >  SRR4302533.5484048/1‑94 (MQ=60)

                                                      ACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGA                                     <  SRR4302533.2794256/94‑1 (MQ=60)

                                                      ACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGA                                     >  SRR4302533.4146781/1‑94 (MQ=60)

                                                      ACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGA                                     <  SRR4302533.3849524/94‑1 (MQ=60)

                                                       CATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAG                                    >  SRR4302533.1975186/1‑94 (MQ=60)

                                                        ATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGC                                   >  SRR4302533.3169566/1‑94 (MQ=60)

                                                         TTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCT                                  <  SRR4302533.4372470/94‑1 (MQ=60)

                                                         TTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCT                                  <  SRR4302533.5624512/94‑1 (MQ=60)

                                                           CTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGG                                >  SRR4302533.4707913/1‑94 (MQ=60)

                                                            TCGAAGCGATTCGTGATCTCCAGGTGGTGATTGGCGTCGGGTAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGG                               <  SRR4302533.1945562/94‑1 (MQ=60)

                                                            TCGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGG                               >  SRR4302533.3826084/1‑94 (MQ=60)

                                                             CGAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGGTCTGGTAAAACGACTCAGTTACCGCAAATCTGTATGGAGCTGGGG                              >  SRR4302533.2711301/1‑94 (MQ=60)

                                                              GAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGC                             >  SRR4302533.3390010/1‑94 (MQ=60)

                                                              GAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGC                             >  SRR4302533.1472411/1‑94 (MQ=60)

                                                              GAAGCGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGC                             >  SRR4302533.4932549/1‑94 (MQ=60)

                                                                  CGATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGG                         <  SRR4302533.3601564/94‑1 (MQ=60)

                                                                    ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGA                       >  SRR4302533.2255671/1‑94 (MQ=60)

                                                                    ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGA                       >  SRR4302533.5781700/1‑94 (MQ=60)

                                                                    ATTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGA                       >  SRR4302533.2047212/1‑94 (MQ=60)

                                                                     TTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGAT                      >  SRR4302533.4715672/1‑94 (MQ=60)

                                                                     TTCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGGTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGAT                      >  SRR4302533.4060170/1‑94 (MQ=60)

                                                                      TCGTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATT                     <  SRR4302533.148107/94‑1 (MQ=60)

                                                                        GTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAA                   >  SRR4302533.2517431/1‑94 (MQ=60)

                                                                        GTGATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAA                   >  SRR4302533.1263254/1‑94 (MQ=60)

                                                                          GATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAG                 >  SRR4302533.2542810/1‑94 (MQ=60)

                                                                           ATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGG                <  SRR4302533.1523146/94‑1 (MQ=60)

                                                                           ATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGG                >  SRR4302533.5734695/1‑94 (MQ=60)

                                                                           ATCACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGG                >  SRR4302533.3831039/1‑94 (MQ=60)

                                                                             CACCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGAC              >  SRR4302533.2864521/1‑94 (MQ=60)

                                                                               CCAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTG            >  SRR4302533.3450461/1‑94 (MQ=60)

                                                                                CAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGA           <  SRR4302533.1241289/94‑1 (MQ=60)

                                                                                CAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGA           >  SRR4302533.1486555/1‑94 (MQ=60)

                                                                                CAGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGA           <  SRR4302533.4436779/94‑1 (MQ=60)

                                                                                 AGGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGAT          <  SRR4302533.862202/94‑1 (MQ=60)

                                                                                  GGTGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATC         <  SRR4302533.1225290/94‑1 (MQ=60)

                                                                                  GGTGGTGATTGTCGCCGGGGAAACGGGTTCCGGTAAAACGACTCAGTTACCGAAAATCTGTATGGCGCTGGGGCGCGGGATTAAAGGACTGATC         >  SRR4302533.3813765/1‑94 (MQ=60)

                                                                                    TGGTGATTGTCGCGGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGG       <  SRR4302533.4022866/94‑1 (MQ=60)

                                                                                    TGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGG       <  SRR4302533.1469668/94‑1 (MQ=60)

                                                                                    TGGTGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGG       <  SRR4302533.1450714/94‑1 (MQ=60)

                                                                                       TGATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCA    >  SRR4302533.5162064/1‑94 (MQ=60)

                                                                                        GATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCAT   >  SRR4302533.1099993/1‑94 (MQ=60)

                                                                                        GATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCAT   >  SRR4302533.5832509/1‑94 (MQ=60)

                                                                                         ATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATatacagca  >  SRR4302533.51626/1‑86 (MQ=60)

                                                                                         ATTGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATA  <  SRR4302533.3593143/94‑1 (MQ=60)

CACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGGGAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGGATTAAAGGACTGATCGGCCATA  >  NC_000913/1481284‑1481466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`