Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,490,230 T→C intergenic (‑77/‑264) b1420 ← / → trg hypothetical protein/methyl‑accepting chemotaxis protein III

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,490,2300TC99.5% 705.2 / NA 218intergenic (‑77/‑264)b1420/trghypothetical protein/methyl‑accepting chemotaxis protein III
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (106/110);  minor base G (0/1);  total (107/111)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 230 total aligned reads displayed.

ATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGTCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTC  >  NC_000913/1490140‑1490318
                                                                                          |                                                                                        
aTTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGcct                                                                                        <  2:120770/93‑1 (MQ=255)
 ttGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGcctc                                                                                       <  1:1324607/93‑1 (MQ=255)
   gCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAg                                                                                     <  2:3744863/93‑1 (MQ=255)
   gCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAg                                                                                     <  2:5594894/93‑1 (MQ=255)
   gCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAg                                                                                     <  2:5850420/93‑1 (MQ=255)
    cTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAgg                                                                                    >  1:445940/1‑93 (MQ=255)
     tAGTTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGc                                                                                   <  1:5790639/93‑1 (MQ=255)
     tAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGc                                                                                   <  1:6276497/93‑1 (MQ=255)
     tAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGc                                                                                   >  2:2463934/1‑93 (MQ=255)
     tAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGc                                                                                   >  1:1499138/1‑93 (MQ=255)
      aGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCa                                                                                  >  2:1319590/1‑93 (MQ=255)
       gcttCATTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCAc                                                                                 <  1:4674917/91‑1 (MQ=255)
        gTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCAcc                                                                                >  2:4768804/1‑93 (MQ=255)
        gTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCAcc                                                                                <  1:4349217/93‑1 (MQ=255)
        gTTCATTCGTTGGCCTAGATTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCAcc                                                                                <  2:2281098/93‑1 (MQ=255)
           cATTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGc                                                                             >  2:369497/1‑93 (MQ=255)
           cATTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGc                                                                             <  2:2724525/93‑1 (MQ=255)
            aTTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCC‑GAAAACCAAAAACCTCCAGCACCCGccg                                                                           >  2:2526523/1‑91 (MQ=255)
             ttCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAg                                                                           <  1:5949633/93‑1 (MQ=255)
             ttCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAg                                                                           >  1:4915726/1‑93 (MQ=255)
             ttCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAg                                                                           >  1:824947/1‑93 (MQ=255)
                 ttGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCAt                                                                       >  2:138367/1‑93 (MQ=255)
                   ggCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTTAAAACAGAAAGCCTCAGGCACCCGCAGGCATcc                                                                     >  1:2150300/1‑93 (MQ=255)
                    gCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCt                                                                    >  1:3917630/1‑93 (MQ=255)
                     ccTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTa                                                                   <  2:6139711/93‑1 (MQ=255)
                       tCGGTTGATAGAAATATCGGTCGTGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATg                                                                 <  1:350758/93‑1 (MQ=255)
                       tCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATg                                                                 >  1:17924/1‑93 (MQ=255)
                        cGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGa                                                                <  2:5994671/93‑1 (MQ=255)
                        cGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGa                                                                >  2:3139485/1‑93 (MQ=255)
                         ggTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAg                                                               <  1:5975893/93‑1 (MQ=255)
                         ggTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAACCTCAGGCACCCGCAGGCATCCTATGAg                                                               >  1:3343075/1‑93 (MQ=255)
                          gTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAgg                                                              >  1:1404572/1‑93 (MQ=255)
                          gTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAgg                                                              >  2:1595436/1‑93 (MQ=255)
                          gTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAgg                                                              <  2:530443/93‑1 (MQ=255)
                           ttGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGt                                                             <  1:3228432/93‑1 (MQ=255)
                           ttGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGt                                                             <  1:4994983/93‑1 (MQ=255)
                            tGATCGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGtt                                                            <  1:6301793/93‑1 (MQ=255)
                            tGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGtt                                                            <  2:5963912/93‑1 (MQ=255)
                             gATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGttt                                                           >  2:1567626/1‑93 (MQ=255)
                                 gAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCtt                                                       >  1:5092496/1‑93 (MQ=255)
                                 gAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCtt                                                       <  1:5134159/93‑1 (MQ=255)
                                  aaaTATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTa                                                      <  2:5435686/93‑1 (MQ=255)
                                  aaaTATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTa                                                      <  2:3943674/93‑1 (MQ=255)
                                  aaaTATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTa                                                      <  2:3492175/93‑1 (MQ=255)
                                  aaaTATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGCTTCCTTa                                                      <  2:2351771/93‑1 (MQ=255)
                                   aaTATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAg                                                     >  1:1992023/1‑93 (MQ=255)
                                   aaTATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAg                                                     <  1:302523/93‑1 (MQ=255)
                                    atatCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAgg                                                    <  2:583206/93‑1 (MQ=255)
                                    atatCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAgg                                                    <  2:1292139/93‑1 (MQ=255)
                                    atatCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAgg                                                    <  1:1216447/93‑1 (MQ=255)
                                     tatCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAggg                                                   >  1:3775388/1‑93 (MQ=255)
                                       tcggtcggGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGAc                                                 <  2:2174289/93‑1 (MQ=255)
                                         ggtcggGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGa                                               >  2:1136116/1‑93 (MQ=255)
                                             ggggCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAaat                                           <  1:207560/93‑1 (MQ=255)
                                             ggggCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAaat                                           <  2:1853504/93‑1 (MQ=255)
                                             ggggCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAaat                                           >  2:2029990/1‑93 (MQ=255)
                                              gggCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAaata                                          <  1:2315085/93‑1 (MQ=255)
                                              gggCCTTCGTCTTTCTGATTACCAGTTAGCCTGAAAACAGAAACACTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAaata                                          <  2:4732942/93‑1 (MQ=255)
                                               ggCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAaataa                                         <  1:868645/93‑1 (MQ=255)
                                                 ccTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATc                                       <  1:5342563/93‑1 (MQ=255)
                                                  cTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCa                                      >  2:5015169/1‑93 (MQ=255)
                                                   ttCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCAc                                     >  1:2339686/1‑93 (MQ=255)
                                                   ttCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCAc                                     >  1:3407264/1‑93 (MQ=255)
                                                   ttCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCAc                                     >  1:4270258/1‑93 (MQ=255)
                                                     cGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACtt                                   >  2:3660870/1‑93 (MQ=255)
                                                       tCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCa                                 <  1:5705749/93‑1 (MQ=255)
                                                       tCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCa                                 >  1:2386350/1‑93 (MQ=255)
                                                          ttCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGa                              >  2:6127126/1‑93 (MQ=255)
                                                             tGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAAACACTTCACGAAAt                           >  1:2873161/1‑93 (MQ=255)
                                                              gATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAAtt                          >  1:4313876/1‑93 (MQ=255)
                                                              gATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAAtt                          >  1:3625347/1‑93 (MQ=255)
                                                               aTTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTg                         <  2:6201921/93‑1 (MQ=255)
                                                                ttCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGc                        >  2:1955212/1‑93 (MQ=255)
                                                                ttCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGc                        >  1:4154411/1‑93 (MQ=255)
                                                                ttCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGc                        <  1:1884489/93‑1 (MQ=255)
                                                                  cccGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGt                      <  1:282722/93‑1 (MQ=255)
                                                                   ccGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTg                     <  2:4163597/93‑1 (MQ=255)
                                                                     ggTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCt                   >  2:944928/1‑93 (MQ=255)
                                                                     ggTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCt                   <  2:1699452/93‑1 (MQ=255)
                                                                      gTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTg                  >  1:5538255/1‑93 (MQ=255)
                                                                       ttAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGt                 <  2:4462537/93‑1 (MQ=255)
                                                                        tAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGtt                >  1:5413337/1‑93 (MQ=255)
                                                                        tAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGtt                >  1:6126180/1‑93 (MQ=255)
                                                                         aGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGttt               >  1:1102805/1‑93 (MQ=255)
                                                                         aGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGttt               >  2:3016261/1‑93 (MQ=255)
                                                                          gCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGtttt              >  1:5741239/1‑93 (MQ=255)
                                                                           ccTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTc             <  1:2889628/93‑1 (MQ=255)
                                                                           ccTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTc             <  2:5755883/93‑1 (MQ=255)
                                                                            cTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTcc            >  2:3406053/1‑93 (MQ=255)
                                                                            cTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTcc            <  1:2552833/93‑1 (MQ=255)
                                                                            cTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTcc            <  2:3874489/93‑1 (MQ=255)
                                                                              gAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAg          >  1:4094537/1‑93 (MQ=255)
                                                                                aaaCAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGaa        <  1:3764659/93‑1 (MQ=255)
                                                                                 aaCAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAAt       >  1:448166/1‑93 (MQ=255)
                                                                                 aaCAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAAt       <  2:2442717/93‑1 (MQ=255)
                                                                                 aaCAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGCTTCCTTAGGGACGAAAATAATCACTTCACGACATTGCGTGCTGTTTTCCAGAAt       >  2:719282/1‑93 (MQ=255)
                                                                                   cAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAAttt     >  2:822807/1‑93 (MQ=255)
                                                                                    aGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAAtttt    >  1:356817/1‑93 (MQ=255)
                                                                                     gAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAAttttt   <  2:5153410/93‑1 (MQ=255)
                                                                                      aaaGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTCCACGAAATTGCGTGCTGTTTTCCAGAATTTTTc  <  2:1919965/93‑1 (MQ=255)
                                                                                          |                                                                                        
ATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGTCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTC  >  NC_000913/1490140‑1490318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGTCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCA  >  NC_000913/1490138‑1490322
                                                                                            |                                                                                            
GAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCC                                                                                             <  SRR4302533.336105/94‑1 (MQ=60)
 AATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCT                                                                                            <  SRR4302533.1577433/94‑1 (MQ=60)
   TTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCA                                                                                          >  SRR4302533.3392454/1‑94 (MQ=60)
   TTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCA                                                                                          <  SRR4302533.1332554/94‑1 (MQ=60)
    TGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAG                                                                                         >  SRR4302533.2671995/1‑94 (MQ=60)
     GCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGG                                                                                        <  SRR4302533.5083262/94‑1 (MQ=60)
     GCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGG                                                                                        >  SRR4302533.448609/1‑94 (MQ=60)
      CTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGC                                                                                       >  SRR4302533.5781428/1‑94 (MQ=60)
      CTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGC                                                                                       >  SRR4302533.1508174/1‑94 (MQ=60)
       TAGTTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCA                                                                                      <  SRR4302533.5825623/94‑1 (MQ=60)
       TAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCA                                                                                      <  SRR4302533.6313976/94‑1 (MQ=60)
         GCTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACC                                                                                    <  SRR4302533.4703244/94‑1 (MQ=60)
          GTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCC                                                                                   <  SRR4302533.4375439/94‑1 (MQ=60)
           GTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCG                                                                                  <  SRR4302533.3224198/94‑1 (MQ=60)
            TCATTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGC                                                                                 <  SRR4302533.2395948/94‑1 (MQ=60)
              ATTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAG                                                                               >  SRR4302533.4945502/1‑94 (MQ=60)
              ATTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAG                                                                               <  SRR4302533.4701208/94‑1 (MQ=60)
              ATTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAG                                                                               >  SRR4302533.829788/1‑94 (MQ=60)
               TTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGG                                                                              <  SRR4302533.5985617/94‑1 (MQ=60)
               TTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGG                                                                              >  SRR4302533.4247913/1‑94 (MQ=60)
                  GTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCAT                                                                           <  SRR4302533.3793595/94‑1 (MQ=60)
                  GTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCAT                                                                           <  SRR4302533.6294945/94‑1 (MQ=60)
                  GTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTAGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCAT                                                                           >  SRR4302533.549380/1‑94 (MQ=60)
                   TTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATC                                                                          <  SRR4302533.2905644/94‑1 (MQ=60)
                    TGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTTAAAACAGAAAGCCTCAGGCACCCGCAGGCATCC                                                                         >  SRR4302533.2163541/1‑94 (MQ=60)
                     GGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCT                                                                        >  SRR4302533.3941555/1‑94 (MQ=60)
                     GGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCT                                                                        <  SRR4302533.5603209/94‑1 (MQ=60)
                       CCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTAT                                                                      >  SRR4302533.3891611/1‑94 (MQ=60)
                       CCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTAT                                                                      <  SRR4302533.1952422/94‑1 (MQ=60)
                        CTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATG                                                                     <  SRR4302533.2050020/94‑1 (MQ=60)
                        CTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATG                                                                     >  SRR4302533.18046/1‑94 (MQ=60)
                         ccgttgGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGA                                                                    <  SRR4302533.2284427/88‑1 (MQ=60)
                         TCGGTTGATAGAAATATCGGTCGTGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGA                                                                    <  SRR4302533.352894/94‑1 (MQ=60)
                         TCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGA                                                                    <  SRR4302533.4369750/94‑1 (MQ=60)
                          CGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAG                                                                   >  SRR4302533.903555/1‑94 (MQ=60)
                          CGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAG                                                                   <  SRR4302533.6133392/94‑1 (MQ=60)
                          CGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAACCTCAGGCACCCGCAGGCATCCTATGAG                                                                   >  SRR4302533.3363735/1‑94 (MQ=60)
                           GGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGG                                                                  <  SRR4302533.6012069/94‑1 (MQ=60)
                           GGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGG                                                                  >  SRR4302533.1413071/1‑94 (MQ=60)
                             TTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTT                                                                >  SRR4302533.4095317/1‑94 (MQ=60)
                             TTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTT                                                                <  SRR4302533.5025264/94‑1 (MQ=60)
                             TTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTT                                                                <  SRR4302533.3248334/94‑1 (MQ=60)
                              TGATCGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTT                                                               <  SRR4302533.6339437/94‑1 (MQ=60)
                              TGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCGTCAGGCACCCGCAGGCATCCTATGAGGTTT                                                               >  SRR4302533.1487758/1‑94 (MQ=60)
                               CATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTC                                                              <  SRR4302533.5064355/94‑1 (MQ=60)
                                ATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCT                                                             >  SRR4302533.1979682/1‑94 (MQ=60)
                                 TAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCT                                                            >  SRR4302533.5445832/1‑94 (MQ=60)
                                  AGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTT                                                           <  SRR4302533.1162769/94‑1 (MQ=60)
                                  AGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTT                                                           >  SRR4302533.5123262/1‑94 (MQ=60)
                                  AGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTT                                                           >  SRR4302533.1520065/1‑94 (MQ=60)
                                  AGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTT                                                           >  SRR4302533.2091452/1‑94 (MQ=60)
                                   GAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTA                                                          <  SRR4302533.5165177/94‑1 (MQ=60)
                                    AAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAG                                                         >  SRR4302533.2004233/1‑94 (MQ=60)
                                    AAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAG                                                         <  SRR4302533.83205/94‑1 (MQ=60)
                                     AATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGG                                                        >  SRR4302533.4637077/1‑94 (MQ=60)
                                     AATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGG                                                        >  SRR4302533.5757581/1‑94 (MQ=60)
                                     AATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGG                                                        <  SRR4302533.304373/94‑1 (MQ=60)
                                      ATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGG                                                       <  SRR4302533.1223753/94‑1 (MQ=60)
                                      ATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGG                                                       >  SRR4302533.746561/1‑94 (MQ=60)
                                      ATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGG                                                       >  SRR4302533.3798531/1‑94 (MQ=60)
                                        ATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGAC                                                     <  SRR4302533.5212308/94‑1 (MQ=60)
                                         TCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACG                                                    <  SRR4302533.613472/94‑1 (MQ=60)
                                           GGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAA                                                  >  SRR4302533.3460125/1‑94 (MQ=60)
                                            GTCGGGTCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAA                                                 <  SRR4302533.1857220/94‑1 (MQ=60)
                                            GTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAA                                                 <  SRR4302533.3586920/94‑1 (MQ=60)
                                              CGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAAT                                               <  SRR4302533.4507130/94‑1 (MQ=60)
                                               GGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATA                                              >  SRR4302533.2880763/1‑94 (MQ=60)
                                               GGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATA                                              <  SRR4302533.208875/94‑1 (MQ=60)
                                                GGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAA                                             <  SRR4302533.2329424/94‑1 (MQ=60)
                                                 GGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAAT                                            <  SRR4302533.873741/94‑1 (MQ=60)
                                                   CCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAACGGCTCAGGCACCTGCAGGCATGCTATGAGGTTTCCTTAGGGACGAAAATAATCA                                          >  SRR4302533.3720886/1‑94 (MQ=60)
                                                   CCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCA                                          >  SRR4302533.3627066/1‑94 (MQ=60)
                                                   CCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCA                                          <  SRR4302533.5374602/94‑1 (MQ=60)
                                                   CCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCA                                          <  SRR4302533.1466760/94‑1 (MQ=60)
                                                    CTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCAC                                         >  SRR4302533.2354175/1‑94 (MQ=60)
                                                    CTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCAC                                         >  SRR4302533.3428309/1‑94 (MQ=60)
                                                    CTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCAC                                         >  SRR4302533.216420/1‑94 (MQ=60)
                                                    CTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCAC                                         >  SRR4302533.4295963/1‑94 (MQ=60)
                                                       CGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTC                                      <  SRR4302533.447406/94‑1 (MQ=60)
                                                        GTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGTCACAGGCACCCGCAGGAATCCGATGAGGTTTCCTTAGGGACGAAAATAATCACTTCA                                     <  SRR4302533.4068687/94‑1 (MQ=60)
                                                        GTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCA                                     <  SRR4302533.5998889/94‑1 (MQ=60)
                                                        GTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCA                                     >  SRR4302533.2401096/1‑94 (MQ=60)
                                                         TCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCAC                                    <  SRR4302533.5740212/94‑1 (MQ=60)
                                                         TCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCAC                                    <  SRR4302533.2441737/94‑1 (MQ=60)
                                                           CTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGA                                  >  SRR4302533.3385976/1‑94 (MQ=60)
                                                            TTCTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAA                                 <  SRR4302533.4335717/94‑1 (MQ=60)
                                                              CTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAAT                               >  SRR4302533.3283545/1‑94 (MQ=60)
                                                              CTGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAAACACTTCACGAAAT                               >  SRR4302533.2890840/1‑94 (MQ=60)
                                                               TGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATT                              >  SRR4302533.3647653/1‑94 (MQ=60)
                                                               TGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATT                              >  SRR4302533.4339846/1‑94 (MQ=60)
                                                               TGATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATT                              <  SRR4302533.5391476/94‑1 (MQ=60)
                                                                GATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTG                             <  SRR4302533.474226/94‑1 (MQ=60)
                                                                 ATTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGC                            >  SRR4302533.4179585/1‑94 (MQ=60)
                                                                  TTCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCG                           <  SRR4302533.1895997/94‑1 (MQ=60)
                                                                   TCCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGT                          <  SRR4302533.2672882/94‑1 (MQ=60)
                                                                    CCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTG                         <  SRR4302533.1282931/94‑1 (MQ=60)
                                                                    CCCGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTG                         <  SRR4302533.284454/94‑1 (MQ=60)
                                                                      CGGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCT                       >  SRR4302533.5158624/1‑94 (MQ=60)
                                                                       GGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTG                      <  SRR4302533.3691879/94‑1 (MQ=60)
                                                                       GGTTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTG                      >  SRR4302533.5571700/1‑94 (MQ=60)
                                                                         TTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTT                    <  SRR4302533.6319437/94‑1 (MQ=60)
                                                                         TTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTT                    >  SRR4302533.5445889/1‑94 (MQ=60)
                                                                         TTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTT                    <  SRR4302533.2197065/94‑1 (MQ=60)
                                                                         TTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTT                    <  SRR4302533.1391641/94‑1 (MQ=60)
                                                                         TTAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTT                    >  SRR4302533.6163104/1‑94 (MQ=60)
                                                                          TAGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTT                   >  SRR4302533.1109335/1‑94 (MQ=60)
                                                                           AGCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTT                  >  SRR4302533.5775884/1‑94 (MQ=60)
                                                                            GCCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTC                 >  SRR4302533.5865276/1‑94 (MQ=60)
                                                                             CCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCC                <  SRR4302533.2347292/94‑1 (MQ=60)
                                                                             CCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCC                >  SRR4302533.4139932/1‑94 (MQ=60)
                                                                             CCTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCC                <  SRR4302533.2907391/94‑1 (MQ=60)
                                                                              CTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCA               >  SRR4302533.5812540/1‑94 (MQ=60)
                                                                              CTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCA               <  SRR4302533.2568542/94‑1 (MQ=60)
                                                                              CTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCA               <  SRR4302533.2968500/94‑1 (MQ=60)
                                                                              CTGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCA               >  SRR4302533.3043731/1‑94 (MQ=60)
                                                                               TGAAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAG              >  SRR4302533.4119465/1‑94 (MQ=60)
                                                                                 AAAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAT            >  SRR4302533.494679/1‑94 (MQ=60)
                                                                                  AAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAAT           <  SRR4302533.3787735/94‑1 (MQ=60)
                                                                                  AAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAAT           >  SRR4302533.450845/1‑94 (MQ=60)
                                                                                  AAACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAAT           <  SRR4302533.634075/94‑1 (MQ=60)
                                                                                   AACAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATT          >  SRR4302533.4299261/1‑94 (MQ=60)
                                                                                     CAGAAAGCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTT        >  SRR4302533.358992/1‑94 (MQ=60)
                                                                                           GCCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCA  <  SRR4302533.2396383/94‑1 (MQ=60)
                                                                                            |                                                                                            
GAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTCGGGGCCTTCGTCTTTCTGATTCCCGGTTAGCCTGAAAACAGAAAGTCTCAGGCACCCGCAGGCATCCTATGAGGTTTCCTTAGGGACGAAAATAATCACTTCACGAAATTGCGTGCTGTTTTCCAGAATTTTTCGTCA  >  NC_000913/1490138‑1490322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑