Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,492,621 A→G S206P (TCA→CCA)  b1422 ← putative transcriptional regulator LYSR‑type

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,492,6210AG99.5% 663.4 / NA 206S206P (TCA→CCA) b1422putative transcriptional regulator LYSR‑type
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (111/94);  minor base C (0/1);  total (111/95)
Fisher's exact test for biased strand distribution p-value = 4.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 209 total aligned reads displayed.

GAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACTTGCAAGGTCGTC  >  NC_000913/1492531‑1492709
                                                                                          |                                                                                        
gAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCa                                                                                        >  1:3397350/1‑93 (MQ=255)
     aGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGa                                                                                   >  2:3944483/1‑93 (MQ=255)
     aGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGa                                                                                   >  1:2478615/1‑93 (MQ=255)
      gCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGAc                                                                                  <  1:1229287/93‑1 (MQ=255)
      gCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGAc                                                                                  >  2:5565140/1‑93 (MQ=255)
       cGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGAcc                                                                                 <  2:1223049/93‑1 (MQ=255)
        gggTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCa                                                                                >  1:5498821/1‑93 (MQ=255)
        gggTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCa                                                                                >  2:3934620/1‑93 (MQ=255)
         ggTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCaa                                                                               >  2:5045668/1‑93 (MQ=255)
          gTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAAt                                                                              >  2:685193/1‑93 (MQ=255)
          gTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAAt                                                                              <  1:202231/93‑1 (MQ=255)
           tGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATg                                                                             <  2:847443/93‑1 (MQ=255)
            gATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGc                                                                            >  1:612171/1‑93 (MQ=255)
               tAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGa                                                                         <  2:3797067/93‑1 (MQ=255)
               tAGGGCGGACAACCAGCTTCAACGACTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGccta                                                                         <  1:6088625/93‑3 (MQ=255)
                 gggCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGata                                                                       <  2:2087873/93‑1 (MQ=255)
                  ggCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGatat                                                                      >  1:3038/1‑93 (MQ=255)
                  ggCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGatat                                                                      <  1:3066660/93‑1 (MQ=255)
                  ggCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGatat                                                                      >  2:2341699/1‑93 (MQ=255)
                   gCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCATTTCAGGATCTGGCATCCGACCAATGCCGATATc                                                                     <  2:6152927/93‑1 (MQ=255)
                    cGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCg                                                                    <  2:501237/93‑1 (MQ=255)
                    cGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCg                                                                    <  2:3297785/93‑1 (MQ=255)
                      gACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGAt                                                                  >  2:6079924/1‑93 (MQ=255)
                      gACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGCTCAGGATCTGGCATCCGACCAATGCCGATATCGAt                                                                  >  1:527822/1‑93 (MQ=255)
                       aCAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGAtt                                                                 <  2:5887120/93‑1 (MQ=255)
                         aaCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTc                                                               >  1:4623481/1‑93 (MQ=255)
                         aaCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTc                                                               >  1:1406931/1‑93 (MQ=255)
                         aaCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTc                                                               >  2:6273181/1‑93 (MQ=255)
                          aCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTcc                                                              <  2:5840510/93‑1 (MQ=255)
                           ccAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTccc                                                             >  1:1136424/1‑93 (MQ=255)
                           ccAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTccc                                                             <  1:2333922/93‑1 (MQ=255)
                            cAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTcccc                                                            <  2:2285797/93‑1 (MQ=255)
                             aGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCg                                                           <  1:4008663/93‑1 (MQ=255)
                              gCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCgg                                                          >  2:2642820/1‑93 (MQ=255)
                                ttCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGtt                                                        >  1:1114602/1‑93 (MQ=255)
                                ttCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGtt                                                        <  1:4952548/93‑1 (MQ=255)
                                ttCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGtt                                                        >  2:4635727/1‑93 (MQ=255)
                                 tCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGttt                                                       >  1:1835581/1‑93 (MQ=255)
                                   aaCGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGttttt                                                     <  1:180058/93‑1 (MQ=255)
                                   aaCGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGttttt                                                     <  2:5960618/93‑1 (MQ=255)
                                   aaCGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGttttt                                                     >  2:1076476/1‑93 (MQ=255)
                                    aCGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTa                                                    >  1:4321277/1‑93 (MQ=255)
                                    aCGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTa                                                    <  1:2282121/93‑1 (MQ=255)
                                      gATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTaaa                                                  <  2:180955/93‑1 (MQ=255)
                                       aTTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTGAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAAc                                                 >  1:4449148/1‑93 (MQ=255)
                                       aTTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAAc                                                 >  2:1924301/1‑93 (MQ=255)
                                        ttCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAAcc                                                >  2:4551267/1‑93 (MQ=255)
                                        ttCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAAcc                                                >  1:371079/1‑93 (MQ=255)
                                          cAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAAcccc                                              >  2:4302440/1‑92 (MQ=255)
                                          cAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCg                                              <  1:1536445/93‑1 (MQ=255)
                                          cAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCg                                              <  1:2449358/93‑1 (MQ=255)
                                           aaGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGc                                             >  1:4748358/1‑93 (MQ=255)
                                            aGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGACCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGcc                                            >  2:5321156/1‑93 (MQ=255)
                                             gAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCa                                           >  2:4219338/1‑93 (MQ=255)
                                             gAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCa                                           <  2:3634462/93‑1 (MQ=255)
                                              aaaCAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAg                                          <  2:4415913/93‑1 (MQ=255)
                                               aaCAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGa                                         >  1:2434025/1‑93 (MQ=255)
                                                 cagcagTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAAt                                       >  1:2665106/1‑93 (MQ=255)
                                                  agcagTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATc                                      >  1:216686/1‑93 (MQ=255)
                                                   gcagTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCa                                     >  1:983328/1‑93 (MQ=255)
                                                    cagTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCAt                                    <  1:587980/93‑1 (MQ=255)
                                                    cagTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCAt                                    <  2:5121784/93‑1 (MQ=255)
                                                     agTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATa                                   <  2:1936532/93‑1 (MQ=255)
                                                      gTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAg                                  >  2:474428/1‑93 (MQ=255)
                                                      gTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAg                                  >  2:5774521/1‑93 (MQ=255)
                                                      gTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAg                                  >  2:4368721/1‑93 (MQ=255)
                                                      gTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAg                                  <  2:3416453/93‑1 (MQ=255)
                                                       ttCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAgg                                 <  1:767601/93‑1 (MQ=255)
                                                       ttCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAgg                                 <  2:4052748/93‑1 (MQ=255)
                                                         cGTAATTAAGCCCGGTCATCAGTTGAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGt                               <  1:5741271/93‑1 (MQ=255)
                                                           tAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTa                             >  1:3843532/1‑93 (MQ=255)
                                                            aaTTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTAc                            <  2:794780/93‑1 (MQ=255)
                                                              ttAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTc                          >  1:3922113/1‑93 (MQ=255)
                                                                aaGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCAt                        <  1:2778908/93‑1 (MQ=255)
                                                                aaGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCAt                        >  1:5061029/1‑93 (MQ=255)
                                                                  gCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTg                      <  1:377596/93‑1 (MQ=255)
                                                                  gCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTg                      >  2:3242027/1‑93 (MQ=255)
                                                                   cccGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGt                     <  1:4900865/93‑1 (MQ=255)
                                                                   cccGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGt                     <  2:4133040/93‑1 (MQ=255)
                                                                    ccGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTc                    <  1:329569/93‑1 (MQ=255)
                                                                     cGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCg                   >  1:406096/1‑93 (MQ=255)
                                                                     cGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCg                   >  2:54552/1‑93 (MQ=255)
                                                                        tcatcaGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCaa                >  2:1375575/1‑93 (MQ=255)
                                                                         catcaGTTCAGGATCTGCCATCCGACCAATGCCGATATCGATTTCCCTGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAAc               <  2:4810885/93‑1 (MQ=255)
                                                                          atcaGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACt              >  1:4548700/1‑93 (MQ=255)
                                                                          atcaGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACt              <  1:3529494/93‑1 (MQ=255)
                                                                           tcaGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACtt             <  1:5563571/93‑1 (MQ=255)
                                                                           tcaGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACtt             <  2:13036/93‑1 (MQ=255)
                                                                           tcaGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACtt             >  2:3118630/1‑93 (MQ=255)
                                                                           tcaGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACtt             >  1:3200625/1‑93 (MQ=255)
                                                                            caGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACTTg            >  2:6022247/1‑93 (MQ=255)
                                                                               ttCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACTTGCaa         <  2:3336572/93‑1 (MQ=255)
                                                                               ttCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACTTGCaa         <  1:4364596/93‑1 (MQ=255)
                                                                                tCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACTTGCAAg        >  1:5423999/1‑93 (MQ=255)
                                                                                tCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACTTGCAAg        <  1:5576760/93‑1 (MQ=255)
                                                                                  aGGATCTGGCATCCGACCAATGCAGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTAGCTACTTGCAAGgt      >  2:4787399/1‑93 (MQ=255)
                                                                                    gATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACTTGCAAGgtcg    >  2:1690924/1‑93 (MQ=255)
                                                                                      tCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACTTGCAAGgtcgtc  <  1:5016349/93‑1 (MQ=255)
                                                                                      tCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACTTGCAAGgtcgtc  <  2:2866393/93‑1 (MQ=255)
                                                                                      tCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACTTGCAAGgtcgtc  >  1:1360903/1‑93 (MQ=255)
                                                                                          |                                                                                        
GAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACTTGCAAGGTCGTC  >  NC_000913/1492531‑1492709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACTTGCAAGGTCGTCTCTTT  >  NC_000913/1492528‑1492714
                                                                                             |                                                                                             
CCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGG                                                                                               <  SRR4302533.232296/94‑1 (MQ=60)
 CTGAAGTAGCGGGTGATTAGGGCGGACATCCAGCTTCAAAGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGC                                                                                              <  SRR4302533.6096354/94‑1 (MQ=60)
 CTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGC                                                                                              <  SRR4302533.283850/94‑1 (MQ=60)
 CTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGC                                                                                              >  SRR4302533.712024/1‑94 (MQ=60)
  TGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCA                                                                                             >  SRR4302533.3418329/1‑94 (MQ=60)
       TAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGA                                                                                        >  SRR4302533.2493901/1‑94 (MQ=60)
        AGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGAC                                                                                       >  SRR4302533.1141077/1‑94 (MQ=60)
         GCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACC                                                                                      <  SRR4302533.1236647/94‑1 (MQ=60)
          CGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTGATTAAGACCGGTCATCAGTTCAGGATCTGGCATCCGACCA                                                                                     >  SRR4302533.3833644/1‑94 (MQ=60)
          CGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCA                                                                                     >  SRR4302533.4077429/1‑94 (MQ=60)
          CGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCA                                                                                     >  SRR4302533.5531989/1‑94 (MQ=60)
             GTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATG                                                                                  <  SRR4302533.203513/94‑1 (MQ=60)
              TGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGC                                                                                 >  SRR4302533.615771/1‑94 (MQ=60)
              TGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGC                                                                                 >  SRR4302533.3081304/1‑94 (MQ=60)
              TGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGC                                                                                 <  SRR4302533.5053724/94‑1 (MQ=60)
               GATTAGGACGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCATGATCTGGCATCCGACCAATGCG                                                                                >  SRR4302533.5724238/1‑94 (MQ=60)
                  TAGGGCGGACAACCAGCTTCAACGACTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCTAT                                                                             <  SRR4302533.6125431/94‑1 (MQ=60)
                   AGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATA                                                                            >  SRR4302533.4000068/1‑94 (MQ=60)
                    GGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATAT                                                                           <  SRR4302533.4636202/94‑1 (MQ=60)
                    GGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATAT                                                                           >  SRR4302533.3062/1‑94 (MQ=60)
                     GGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATC                                                                          >  SRR4302533.5553405/1‑94 (MQ=60)
                     GGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATC                                                                          <  SRR4302533.3085497/94‑1 (MQ=60)
                     GGCGCACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATC                                                                          <  SRR4302533.2493379/94‑1 (MQ=60)
                      GCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCG                                                                         <  SRR4302533.4394053/94‑1 (MQ=60)
                      GCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCG                                                                         <  SRR4302533.1845035/94‑1 (MQ=60)
                        GGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGAT                                                                       <  SRR4302533.232302/94‑1 (MQ=60)
                        GGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGAT                                                                       >  SRR4302533.2414529/1‑94 (MQ=60)
                        GGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGAT                                                                       >  SRR4302533.3670508/1‑94 (MQ=60)
                        GGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGCTCAGGATCTGGCATCCGACCAATGCCGATATCGAT                                                                       >  SRR4302533.530960/1‑94 (MQ=60)
                          ACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTT                                                                     <  SRR4302533.4385320/94‑1 (MQ=60)
                           CAACCAGCTTCAGCGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTC                                                                    <  SRR4302533.1508652/94‑1 (MQ=60)
                           CAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTC                                                                    >  SRR4302533.1415440/1‑94 (MQ=60)
                           CAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTC                                                                    >  SRR4302533.4651505/1‑94 (MQ=60)
                           CAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTC                                                                    <  SRR4302533.2795242/94‑1 (MQ=60)
                            AACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCC                                                                   >  SRR4302533.5311326/1‑94 (MQ=60)
                            AACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCC                                                                   <  SRR4302533.4137094/94‑1 (MQ=60)
                            AACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCC                                                                   >  SRR4302533.4933316/1‑94 (MQ=60)
                             ACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCC                                                                  >  SRR4302533.1143183/1‑94 (MQ=60)
                             ACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCC                                                                  >  SRR4302533.5953672/1‑94 (MQ=60)
                              CCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCC                                                                 <  SRR4302533.2348373/94‑1 (MQ=60)
                               CAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCG                                                                <  SRR4302533.1721985/94‑1 (MQ=60)
                               CAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCG                                                                >  SRR4302533.3151471/1‑94 (MQ=60)
                                AGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGG                                                               >  SRR4302533.1299487/1‑94 (MQ=60)
                                AGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGG                                                               <  SRR4302533.4033071/94‑1 (MQ=60)
                                AGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGG                                                               >  SRR4302533.5499016/1‑94 (MQ=60)
                                  CTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTT                                                             >  SRR4302533.1121211/1‑94 (MQ=60)
                                   TTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTT                                                            <  SRR4302533.4982544/94‑1 (MQ=60)
                                   TTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTT                                                            >  SRR4302533.1846781/1‑94 (MQ=60)
                                     CAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTT                                                          >  SRR4302533.2457990/1‑94 (MQ=60)
                                     CAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTT                                                          <  SRR4302533.1949648/94‑1 (MQ=60)
                                      AACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTA                                                         <  SRR4302533.181182/94‑1 (MQ=60)
                                      AACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTA                                                         >  SRR4302533.4347304/1‑94 (MQ=60)
                                       ACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAA                                                        <  SRR4302533.2296247/94‑1 (MQ=60)
                                         GATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTGAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAAC                                                      >  SRR4302533.4476056/1‑94 (MQ=60)
                                          ATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACC                                                     >  SRR4302533.373319/1‑94 (MQ=60)
                                            TCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCG                                                   <  SRR4302533.5852950/94‑1 (MQ=60)
                                             CAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGC                                                  <  SRR4302533.2464476/94‑1 (MQ=60)
                                             CAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGC                                                  >  SRR4302533.4777145/1‑94 (MQ=60)
                                             CAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGC                                                  <  SRR4302533.1545676/94‑1 (MQ=60)
                                                GAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAG                                               <  SRR4302533.559965/94‑1 (MQ=60)
                                                 AAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGA                                              >  SRR4302533.2449062/1‑94 (MQ=60)
                                                   ACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAAT                                            <  SRR4302533.5171593/94‑1 (MQ=60)
                                                   ACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAAT                                            >  SRR4302533.2681461/1‑94 (MQ=60)
                                                    CAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATC                                           <  SRR4302533.6153795/94‑1 (MQ=60)
                                                    CAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATC                                           >  SRR4302533.218046/1‑94 (MQ=60)
                                                     AGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCA                                          >  SRR4302533.989093/1‑94 (MQ=60)
                                                     AGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCA                                          >  SRR4302533.2122870/1‑94 (MQ=60)
                                                      GCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCATTGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCAT                                         <  SRR4302533.3397846/94‑1 (MQ=60)
                                                      ACAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCAT                                         <  SRR4302533.4682004/94‑1 (MQ=60)
                                                       CAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATA                                        <  SRR4302533.591450/94‑1 (MQ=60)
                                                        AGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAG                                       <  SRR4302533.1005163/94‑1 (MQ=60)
                                                         GTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGG                                      >  SRR4302533.707618/1‑94 (MQ=60)
                                                          TTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGG                                     <  SRR4302533.772129/94‑1 (MQ=60)
                                                            CGTAATTAAGCCCGGTCATCAGTTGAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTT                                   <  SRR4302533.5775916/94‑1 (MQ=60)
                                                             GTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTA                                  >  SRR4302533.3867010/1‑94 (MQ=60)
                                                             GTAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTA                                  <  SRR4302533.4833907/94‑1 (MQ=60)
                                                              TAATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTAC                                 >  SRR4302533.86361/1‑94 (MQ=60)
                                                                ATTAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTC                               >  SRR4302533.3946065/1‑94 (MQ=60)
                                                                  TAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCAT                             >  SRR4302533.3701066/1‑94 (MQ=60)
                                                                  TAAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCAT                             >  SRR4302533.5091613/1‑94 (MQ=60)
                                                                   AAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATT                            >  SRR4302533.2380835/1‑94 (MQ=60)
                                                                   AAGCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATT                            <  SRR4302533.2795978/94‑1 (MQ=60)
                                                                     GCCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGT                          <  SRR4302533.379870/94‑1 (MQ=60)
                                                                      CCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTC                         <  SRR4302533.4930560/94‑1 (MQ=60)
                                                                      CCCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTC                         >  SRR4302533.4727568/1‑94 (MQ=60)
                                                                       CCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCG                        >  SRR4302533.408536/1‑94 (MQ=60)
                                                                       CCGGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCG                        <  SRR4302533.331581/94‑1 (MQ=60)
                                                                         GGTCATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCA                      >  SRR4302533.2202030/1‑94 (MQ=60)
                                                                            CATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACT                   >  SRR4302533.2548282/1‑94 (MQ=60)
                                                                            CATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACT                   >  SRR4302533.4576249/1‑94 (MQ=60)
                                                                             ATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACTT                  >  SRR4302533.3220319/1‑94 (MQ=60)
                                                                             ATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACTT                  >  SRR4302533.3784012/1‑94 (MQ=60)
                                                                             ATCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACTT                  <  SRR4302533.3551245/94‑1 (MQ=60)
                                                                              TCAGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACTTG                 <  SRR4302533.5597200/94‑1 (MQ=60)
                                                                                AGTTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACTTGCA               >  SRR4302533.1181252/1‑94 (MQ=60)
                                                                                  TTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCTCCGGCTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACTTGCAAG             >  SRR4302533.4549035/1‑94 (MQ=60)
                                                                                  TTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACTTGCAAG             <  SRR4302533.4390942/94‑1 (MQ=60)
                                                                                  TTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACTTGCAAG             >  SRR4302533.5456665/1‑94 (MQ=60)
                                                                                  TTCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACTTGCAAG             >  SRR4302533.2993911/1‑94 (MQ=60)
                                                                                   TCAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACTTGCAAGG            <  SRR4302533.5610463/94‑1 (MQ=60)
                                                                                    CAGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACTTGCAAGGT           <  SRR4302533.3879648/94‑1 (MQ=60)
                                                                                     AGGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACTTGCAAGGTC          >  SRR4302533.5012085/1‑94 (MQ=60)
                                                                                      GGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACTTGCAAGGTCG         <  SRR4302533.1410556/94‑1 (MQ=60)
                                                                                      GGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACTTGCAAGGTCG         >  SRR4302533.5848525/1‑94 (MQ=60)
                                                                                      GGATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACTTGCAAGGTCG         <  SRR4302533.3309939/94‑1 (MQ=60)
                                                                                       GATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACTTGCAAGGTCGT        <  SRR4302533.3513210/94‑1 (MQ=60)
                                                                                        ATCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACTTGCAAGGTCGTC       >  SRR4302533.1369079/1‑94 (MQ=60)
                                                                                         TCTGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACTTGCAAGGTCGTCT      <  SRR4302533.5046738/94‑1 (MQ=60)
                                                                                           TGGCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACTTGCAAGGTCGTCTCT    <  SRR4302533.2217199/94‑1 (MQ=60)
                                                                                             GCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACTTGCAAGGTCGTCTCTTT  <  SRR4302533.167479/94‑1 (MQ=60)
                                                                                             GCATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACTTGCAAGGTCGTCTCTTT  <  SRR4302533.3770615/94‑1 (MQ=60)
                                                                                             |                                                                                             
CCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCATTGTCGCAACTTGCAAGGTCGTCTCTTT  >  NC_000913/1492528‑1492714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑