Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,507,420 T→C T18A (ACG→GCG)  b1437 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,507,4200TC100.0% 716.0 / NA 224T18A (ACG→GCG) b1437hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (109/115);  total (109/115)

Only 100 of 231 total aligned reads displayed.

ACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAAAAAGGTTAATGA  >  NC_000913/1507330‑1507509
                                                                                          |                                                                                         
aCGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCa                                                                                         <  2:2151542/93‑1 (MQ=255)
  gTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCCTTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCAcc                                                                                       >  1:2189321/1‑93 (MQ=255)
  gTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCAcc                                                                                       >  2:1176138/1‑93 (MQ=255)
   ttGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCAccc                                                                                      >  2:5187729/1‑93 (MQ=255)
   ttGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCAccc                                                                                      >  1:2789480/1‑93 (MQ=255)
     ggCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCt                                                                                    <  2:1282294/93‑1 (MQ=255)
     ggCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCt                                                                                    <  2:1434991/93‑1 (MQ=255)
      gCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTg                                                                                   >  1:2299328/1‑93 (MQ=255)
       cTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCTGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCAACCCCTGc                                                                                  <  2:6113665/93‑1 (MQ=255)
       cTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGc                                                                                  <  1:1601769/93‑1 (MQ=255)
         cGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGa                                                                                <  1:979107/93‑1 (MQ=255)
         cGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGa                                                                                >  2:2116535/1‑93 (MQ=255)
         cGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGa                                                                                <  2:5321983/93‑1 (MQ=255)
          gAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGAt                                                                               >  1:2075655/1‑93 (MQ=255)
            aTCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATga                                                                             <  1:5169289/93‑1 (MQ=255)
             tctcAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATgag                                                                            <  1:4773397/93‑1 (MQ=255)
             tctcAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATgag                                                                            <  2:4559603/93‑1 (MQ=255)
              ctcAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATgaga                                                                           <  1:2578953/93‑1 (MQ=255)
              ctcAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATgaga                                                                           >  1:1932540/1‑93 (MQ=255)
               tcAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGAt                                                                          >  1:4338711/1‑93 (MQ=255)
               tcAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGAt                                                                          <  2:843598/93‑1 (MQ=255)
               tcAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGAt                                                                          >  1:4069813/1‑93 (MQ=255)
                 aGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAGCTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTa                                                                        <  1:2986102/93‑1 (MQ=255)
                 aGGGCGTCGATGTAGCGAAGGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTa                                                                        <  2:1551665/93‑1 (MQ=255)
                  gggCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTaa                                                                       <  2:3019096/93‑1 (MQ=255)
                  gggCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTaa                                                                       <  1:1329558/93‑1 (MQ=255)
                   ggCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTaaa                                                                      <  1:31460/93‑1 (MQ=255)
                   ggCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTaaa                                                                      <  1:787508/93‑1 (MQ=255)
                    gCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTaaaa                                                                     >  2:2341780/1‑92 (MQ=255)
                    gCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAg                                                                     <  1:4197738/93‑1 (MQ=255)
                    gCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAg                                                                     <  1:3272111/93‑1 (MQ=255)
                       tCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAAc                                                                  >  2:571757/1‑93 (MQ=255)
                       tCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAAc                                                                  >  2:3360847/1‑93 (MQ=255)
                        cGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAAcc                                                                 <  1:4029080/93‑1 (MQ=255)
                        cGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAAcc                                                                 <  2:5061077/93‑1 (MQ=255)
                        cGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAAcc                                                                 >  1:1491068/1‑93 (MQ=255)
                         gATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCa                                                                <  1:2533040/93‑1 (MQ=255)
                          aTGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCAt                                                               >  1:3226349/1‑93 (MQ=255)
                            gTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTg                                                             >  2:4464075/1‑93 (MQ=255)
                             tAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGc                                                            <  2:5846829/93‑1 (MQ=255)
                             tAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGc                                                            >  1:2284584/1‑93 (MQ=255)
                             tAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGc                                                            >  1:5516094/1‑93 (MQ=255)
                              aGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCg                                                           >  1:4402307/1‑93 (MQ=255)
                               gCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATCGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGt                                                          <  1:1539755/93‑1 (MQ=255)
                                cGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTa                                                         <  2:2830100/93‑1 (MQ=255)
                                 gAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTaa                                                        >  1:4243562/1‑93 (MQ=255)
                                  aaTGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTaaa                                                       <  2:6230718/93‑1 (MQ=255)
                                     ggCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCa                                                    <  1:3470639/93‑1 (MQ=255)
                                       cagcaACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAAt                                                  >  2:3474157/1‑93 (MQ=255)
                                         gcaACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATcc                                                >  2:4840589/1‑93 (MQ=255)
                                            aCCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGa                                             <  1:4787559/93‑1 (MQ=255)
                                            aCCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGa                                             <  2:1930055/93‑1 (MQ=255)
                                             ccATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGaa                                            <  2:5048793/93‑1 (MQ=255)
                                             ccATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGaa                                            >  2:669978/1‑93 (MQ=255)
                                             ccATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGaa                                            >  1:6274392/1‑93 (MQ=255)
                                              cATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGaaa                                           >  2:2718252/1‑93 (MQ=255)
                                              cATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGaaa                                           >  1:5647579/1‑93 (MQ=255)
                                                  tGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAaacaac                                       <  2:557682/93‑1 (MQ=255)
                                                   gAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACt                                      <  1:1958572/93‑1 (MQ=255)
                                                    aaaCTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTc                                     >  2:1311711/1‑93 (MQ=255)
                                                    aaaCTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTc                                     >  1:4965852/1‑93 (MQ=255)
                                                     aaCTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCg                                    >  2:3949390/1‑93 (MQ=255)
                                                      aCTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCgg                                   >  2:5790668/1‑93 (MQ=255)
                                                       cTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGt                                  >  1:2009647/1‑93 (MQ=255)
                                                        tCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGtt                                 >  1:101304/1‑93 (MQ=255)
                                                           ggTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGa                              >  2:4133568/1‑93 (MQ=255)
                                                            gTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAg                             >  1:2684823/1‑93 (MQ=255)
                                                             tttCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGt                            >  1:4422792/1‑93 (MQ=255)
                                                             tttCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGt                            >  1:5962420/1‑93 (MQ=255)
                                                              ttCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGtt                           <  1:2955691/93‑1 (MQ=255)
                                                              ttCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGtt                           >  2:1618952/1‑93 (MQ=255)
                                                               tCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTa                          >  1:1856418/1‑93 (MQ=255)
                                                               tCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTa                          <  1:5451787/93‑1 (MQ=255)
                                                               tCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTa                          >  2:4706454/1‑93 (MQ=255)
                                                                cATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTaa                         <  2:5945362/93‑1 (MQ=255)
                                                                 aTGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAAt                        >  2:2407732/1‑93 (MQ=255)
                                                                 aTGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAAt                        <  2:1821496/93‑1 (MQ=255)
                                                                 aTGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAAt                        >  1:1304837/1‑93 (MQ=255)
                                                                  tGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATc                       >  2:3614854/1‑93 (MQ=255)
                                                                  tGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATc                       >  2:6005889/1‑93 (MQ=255)
                                                                  tGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATc                       >  1:6244420/1‑93 (MQ=255)
                                                                  tGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATc                       <  2:3232398/93‑1 (MQ=255)
                                                                  tGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATc                       >  1:5554340/1‑93 (MQ=255)
                                                                        gcgcAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAAt                 <  2:1384098/93‑1 (MQ=255)
                                                                           cAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTaa              >  1:57740/1‑93 (MQ=255)
                                                                            aGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTaaa             >  1:3878484/1‑93 (MQ=255)
                                                                              tgtCATTCCGCGCCACCCCTGCGATGAGATTAAAGAGCCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGATTTAATCGCCAATTaaaaa           <  1:5003127/93‑1 (MQ=255)
                                                                              tgtCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTaaaaa           >  2:3811534/1‑93 (MQ=255)
                                                                               gtCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAAAAAg          >  2:3687545/1‑93 (MQ=255)
                                                                               gtCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAAAAAg          <  2:4234152/93‑1 (MQ=255)
                                                                                tCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAAAAAgg         <  1:2939002/93‑1 (MQ=255)
                                                                                 cATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAAAAAGGt        >  1:2057338/1‑93 (MQ=255)
                                                                                 cATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAAAAAGGt        <  1:587152/93‑1 (MQ=255)
                                                                                  aTGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAAAAAGGtt       <  1:4830310/93‑1 (MQ=255)
                                                                                  aTGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAAAAAGGtt       <  2:2525764/93‑1 (MQ=255)
                                                                                   tGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAAAAAGGTTa      >  1:732728/1‑93 (MQ=255)
                                                                                    gCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAAAAAGGTTaa     <  2:1694403/93‑1 (MQ=255)
                                                                                     ccGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAAAAAGGTTAAt    <  2:3628197/93‑1 (MQ=255)
                                                                                      cgcgCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAAAAAGGTTAATg   <  1:450875/93‑1 (MQ=255)
                                                                                       gcgcCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAAAAAGGTTAATGa  <  1:1091/93‑1 (MQ=255)
                                                                                          |                                                                                         
ACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAAAAAGGTTAATGA  >  NC_000913/1507330‑1507509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAAAAAGGTTAATGACA  >  NC_000913/1507327‑1507511
                                                                                             |                                                                                           
CAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGC                                                                                             >  SRR4302533.1691073/1‑94 (MQ=60)
CAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGC                                                                                             >  SRR4302533.5230598/1‑94 (MQ=60)
 AGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCC                                                                                            >  SRR4302533.6149674/1‑94 (MQ=60)
   ACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCAC                                                                                          <  SRR4302533.400198/94‑1 (MQ=60)
    CGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCCTTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACC                                                                                         >  SRR4302533.2202809/1‑94 (MQ=60)
     GTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCC                                                                                        >  SRR4302533.2806631/1‑94 (MQ=60)
       TGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCT                                                                                      >  SRR4302533.4366270/1‑94 (MQ=60)
        GGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCGCCCCTG                                                                                     <  SRR4302533.5668415/94‑1 (MQ=60)
        GGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTG                                                                                     >  SRR4302533.2313583/1‑94 (MQ=60)
         GCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGC                                                                                    <  SRR4302533.3199375/94‑1 (MQ=60)
         GCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGC                                                                                    <  SRR4302533.6060249/94‑1 (MQ=60)
          CTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCG                                                                                   <  SRR4302533.1611465/94‑1 (MQ=60)
           TCGAATCTCAGGGCGTCGATTTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGA                                                                                  >  SRR4302533.5367898/1‑94 (MQ=60)
           TCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGA                                                                                  >  SRR4302533.4777978/1‑94 (MQ=60)
            CGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGAT                                                                                 <  SRR4302533.4952774/94‑1 (MQ=60)
            CGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGAT                                                                                 <  SRR4302533.3718106/94‑1 (MQ=60)
            CGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGAT                                                                                 >  SRR4302533.2088432/1‑94 (MQ=60)
            CGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGAT                                                                                 <  SRR4302533.984848/94‑1 (MQ=60)
             GAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATG                                                                                >  SRR4302533.1479165/1‑94 (MQ=60)
               ATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAG                                                                              <  SRR4302533.5200491/94‑1 (MQ=60)
                TCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGA                                                                             >  SRR4302533.1944328/1‑94 (MQ=60)
                TCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGA                                                                             <  SRR4302533.4802321/94‑1 (MQ=60)
                 CTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGAT                                                                            >  SRR4302533.4364859/1‑94 (MQ=60)
                 CTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGAT                                                                            <  SRR4302533.2613811/94‑1 (MQ=60)
                 CTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGAT                                                                            <  SRR4302533.2594824/94‑1 (MQ=60)
                 CTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGAT                                                                            <  SRR4302533.2304067/94‑1 (MQ=60)
                 CTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGAT                                                                            >  SRR4302533.4094588/1‑94 (MQ=60)
                   CAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTGATGCCGCGCCACCCCTGCGATGAGATTA                                                                          >  SRR4302533.6097142/1‑94 (MQ=60)
                    AGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAGCTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAA                                                                         <  SRR4302533.3004371/94‑1 (MQ=60)
                    AGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAA                                                                         <  SRR4302533.5548872/94‑1 (MQ=60)
                     GGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAA                                                                        <  SRR4302533.1337535/94‑1 (MQ=60)
                      GGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAG                                                                       <  SRR4302533.792134/94‑1 (MQ=60)
                      GGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAG                                                                       >  SRR4302533.4477794/1‑94 (MQ=60)
                      GGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAG                                                                       <  SRR4302533.31677/94‑1 (MQ=60)
                      GGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTGTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAG                                                                       >  SRR4302533.3411325/1‑94 (MQ=60)
                       GCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGA                                                                      <  SRR4302533.3292303/94‑1 (MQ=60)
                       GCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGA                                                                      <  SRR4302533.4223148/94‑1 (MQ=60)
                        CGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGAGCCACCCCTGCGATGAGATTAAAGAA                                                                     >  SRR4302533.1038822/1‑94 (MQ=60)
                         GTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAAC                                                                    <  SRR4302533.325480/94‑1 (MQ=60)
                          TCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACC                                                                   >  SRR4302533.1500060/1‑94 (MQ=60)
                           CGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCA                                                                  <  SRR4302533.2100669/94‑1 (MQ=60)
                           CGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCA                                                                  <  SRR4302533.4053602/94‑1 (MQ=60)
                           CGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCA                                                                  >  SRR4302533.696815/1‑94 (MQ=60)
                            GATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCAT                                                                 >  SRR4302533.1463324/1‑94 (MQ=60)
                            GATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCAT                                                                 >  SRR4302533.3246242/1‑94 (MQ=60)
                            GATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCAT                                                                 <  SRR4302533.4175817/94‑1 (MQ=60)
                            GATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCAT                                                                 >  SRR4302533.5134716/1‑94 (MQ=60)
                            GATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCAT                                                                 <  SRR4302533.2548631/94‑1 (MQ=60)
                             ATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATT                                                                <  SRR4302533.2884231/94‑1 (MQ=60)
                             ATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATT                                                                >  SRR4302533.1908392/1‑94 (MQ=60)
                             ATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATT                                                                <  SRR4302533.1654493/94‑1 (MQ=60)
                               GTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGC                                                              >  SRR4302533.2298723/1‑94 (MQ=60)
                               GTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGC                                                              >  SRR4302533.5549395/1‑94 (MQ=60)
                                TAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCG                                                             >  SRR4302533.4428887/1‑94 (MQ=60)
                                 AGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGT                                                            >  SRR4302533.2962419/1‑94 (MQ=60)
                                 AGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGT                                                            >  SRR4302533.527234/1‑94 (MQ=60)
                                  GCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGACGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTA                                                           >  SRR4302533.3138014/1‑94 (MQ=60)
                                  GCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATCGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTA                                                           <  SRR4302533.1549028/94‑1 (MQ=60)
                                   CGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAA                                                          >  SRR4302533.4269188/1‑94 (MQ=60)
                                   CGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAA                                                          >  SRR4302533.1108690/1‑94 (MQ=60)
                                    GAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAA                                                         >  SRR4302533.5953760/1‑94 (MQ=60)
                                       TGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCA                                                      <  SRR4302533.4262853/94‑1 (MQ=60)
                                       TGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCA                                                      <  SRR4302533.4844143/94‑1 (MQ=60)
                                        GGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAA                                                     <  SRR4302533.3492064/94‑1 (MQ=60)
                                         GCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAAT                                                    >  SRR4302533.2562752/1‑94 (MQ=60)
                                         GCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAAT                                                    <  SRR4302533.5562630/94‑1 (MQ=60)
                                           AGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCC                                                  >  SRR4302533.4420790/1‑94 (MQ=60)
                                           AGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCC                                                  <  SRR4302533.3106400/94‑1 (MQ=60)
                                           AGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCC                                                  >  SRR4302533.1573615/1‑94 (MQ=60)
                                             CAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTG                                                <  SRR4302533.4059381/94‑1 (MQ=60)
                                               ACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAA                                              >  SRR4302533.4963790/1‑94 (MQ=60)
                                               ACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAA                                              <  SRR4302533.4816555/94‑1 (MQ=60)
                                               ACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAA                                              >  SRR4302533.6311866/1‑94 (MQ=60)
                                                CCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAA                                             >  SRR4302533.4185904/1‑94 (MQ=60)
                                                CCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAA                                             >  SRR4302533.5681720/1‑94 (MQ=60)
                                                  ATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACA                                           <  SRR4302533.2982732/94‑1 (MQ=60)
                                                  ATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACA                                           >  SRR4302533.2828749/1‑94 (MQ=60)
                                                     TGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACT                                        <  SRR4302533.1975168/94‑1 (MQ=60)
                                                     TGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACT                                        <  SRR4302533.2071866/94‑1 (MQ=60)
                                                      GAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTC                                       >  SRR4302533.4995955/1‑94 (MQ=60)
                                                      GAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTC                                       <  SRR4302533.1970527/94‑1 (MQ=60)
                                                         ACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGT                                    >  SRR4302533.2022026/1‑94 (MQ=60)
                                                         ACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGT                                    <  SRR4302533.759957/94‑1 (MQ=60)
                                                          CTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTT                                   >  SRR4302533.101933/1‑94 (MQ=60)
                                                              GGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAG                               >  SRR4302533.2701275/1‑94 (MQ=60)
                                                              GGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAG                               >  SRR4302533.851623/1‑94 (MQ=60)
                                                              GGTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAG                               <  SRR4302533.3407405/94‑1 (MQ=60)
                                                               GTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGT                              >  SRR4302533.4449532/1‑94 (MQ=60)
                                                               GTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGT                              >  SRR4302533.5998490/1‑94 (MQ=60)
                                                               GTTTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGT                              <  SRR4302533.1331116/94‑1 (MQ=60)
                                                                 TTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTA                            <  SRR4302533.2973763/94‑1 (MQ=60)
                                                                 TTCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTA                            >  SRR4302533.1867741/1‑94 (MQ=60)
                                                                  TCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAA                           <  SRR4302533.196641/94‑1 (MQ=60)
                                                                  TCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAA                           <  SRR4302533.152714/94‑1 (MQ=60)
                                                                  TCATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAA                           <  SRR4302533.5484628/94‑1 (MQ=60)
                                                                   CATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAAT                          >  SRR4302533.5818975/1‑94 (MQ=60)
                                                                   CATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAAT                          >  SRR4302533.5761882/1‑94 (MQ=60)
                                                                   CATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAAT                          >  SRR4302533.1312679/1‑94 (MQ=60)
                                                                    ATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATC                         >  SRR4302533.5587884/1‑94 (MQ=60)
                                                                    ATGGGAGGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATC                         >  SRR4302533.6281797/1‑94 (MQ=60)
                                                                          GGCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAAT                   >  SRR4302533.2116674/1‑94 (MQ=60)
                                                                           GCGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATT                  <  SRR4302533.279705/94‑1 (MQ=60)
                                                                            CGCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTA                 >  SRR4302533.2050325/1‑94 (MQ=60)
                                                                             GCAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAA                >  SRR4302533.58122/1‑94 (MQ=60)
                                                                              CAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAAA               >  SRR4302533.2254902/1‑94 (MQ=60)
                                                                              CAGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAAA               >  SRR4302533.3902153/1‑94 (MQ=60)
                                                                               AGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAAAA              >  SRR4302533.4809094/1‑94 (MQ=60)
                                                                               AGTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAAAA              >  SRR4302533.5729612/1‑94 (MQ=60)
                                                                                GTGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAAAAA             <  SRR4302533.779234/94‑1 (MQ=60)
                                                                                 TGTCATTCCGCGCCACCCCTGCGATGAGATTAAAGAGCCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGATTTAATCGCCAATTAAAAAG            <  SRR4302533.5033451/94‑1 (MQ=60)
                                                                                 TGTCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAAAAAG            <  SRR4302533.1626901/94‑1 (MQ=60)
                                                                                   TCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAAAAAGGT          <  SRR4302533.2956959/94‑1 (MQ=60)
                                                                                   TCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAAAAAGGT          <  SRR4302533.1987654/94‑1 (MQ=60)
                                                                                   TCATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAAAAAGGT          >  SRR4302533.2070026/1‑94 (MQ=60)
                                                                                    CATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAAAAAGGTT         <  SRR4302533.5025530/94‑1 (MQ=60)
                                                                                    CATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAAAAAGGTT         <  SRR4302533.590620/94‑1 (MQ=60)
                                                                                    CATACCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAAAAAGGTT         <  SRR4302533.2570871/94‑1 (MQ=60)
                                                                                     ATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAAAAAGGTTA        >  SRR4302533.737038/1‑94 (MQ=60)
                                                                                     ATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAAAAAGGTTA        <  SRR4302533.4435871/94‑1 (MQ=60)
                                                                                     ATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAAAAAGGTTA        <  SRR4302533.2495068/94‑1 (MQ=60)
                                                                                     ATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAAAAAGGTTA        <  SRR4302533.4859586/94‑1 (MQ=60)
                                                                                     ATGCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAAAAAGGTTA        <  SRR4302533.4330066/94‑1 (MQ=60)
                                                                                       GCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAAAAAGGTTAAT      <  SRR4302533.6310630/94‑1 (MQ=60)
                                                                                       GCCGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAAAAAGGTTAAT      >  SRR4302533.4572599/1‑94 (MQ=60)
                                                                                         CGCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAAAAAGGTTAATGA    <  SRR4302533.453574/94‑1 (MQ=60)
                                                                                          GCGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAAAAAGGTTAATGAC   <  SRR4302533.1102/94‑1 (MQ=60)
                                                                                           CGCCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAAAAAGGTTAATGACA  <  SRR4302533.100888/94‑1 (MQ=60)
                                                                                             |                                                                                           
CAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAGGTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAACAACTCGGTTTGAGTTAATCGCCAATTAAAAAGGTTAATGACA  >  NC_000913/1507327‑1507511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑