Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,562,886 A→G V299A (GTT→GCT)  b1489 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,562,8860AG100.0% 702.3 / NA 219V299A (GTT→GCT) b1489hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (99/120);  total (99/120)

Only 100 of 227 total aligned reads displayed.

GCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑CCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAG  >  NC_000913/1562796‑1562974
                                                                                          |                                                                                          
  tGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATg                                                                                        >  1:3503999/1‑93 (MQ=255)
   gACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGc                                                                                       <  2:754013/93‑1 (MQ=255)
   gACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGc                                                                                       <  2:2039347/93‑1 (MQ=255)
    aCCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCg                                                                                      >  1:967492/1‑93 (MQ=255)
     ccAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGa                                                                                     >  1:2420694/1‑93 (MQ=255)
      cAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGAt                                                                                    >  1:761296/1‑93 (MQ=255)
      cAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGAt                                                                                    <  1:263615/93‑1 (MQ=255)
        gCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTc                                                                                  <  2:3044217/93‑1 (MQ=255)
          tttGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGt                                                                                >  1:1190324/1‑93 (MQ=255)
          tttGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGt                                                                                >  2:5883467/1‑93 (MQ=255)
          tttGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGt                                                                                >  1:2522788/1‑93 (MQ=255)
             gcgcATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGa                                                                             >  2:670456/1‑93 (MQ=255)
              cgcATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAg                                                                            >  2:4857813/1‑93 (MQ=255)
               gcATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAga                                                                           >  2:263156/1‑92 (MQ=255)
               gcATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGt                                                                           >  1:5479297/1‑93 (MQ=255)
               gcATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGt                                                                           >  1:1952174/1‑93 (MQ=255)
                cATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTa                                                                          <  2:890310/93‑1 (MQ=255)
                 aTTTTGAATTTCTACACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTAc                                                                         <  1:3600831/93‑1 (MQ=255)
                  ttttGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACa                                                                        <  2:5002777/93‑1 (MQ=255)
                   tttGAATGTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAg                                                                       <  1:3200338/93‑1 (MQ=255)
                    ttGAATTTCTGCGCCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGa                                                                      <  2:1263022/93‑1 (MQ=255)
                    ttGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGa                                                                      >  1:921855/1‑93 (MQ=255)
                     tGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGAt                                                                     <  2:2761052/93‑1 (MQ=255)
                     tGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGAt                                                                     <  2:1044812/93‑1 (MQ=255)
                       aaTTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTc                                                                   <  1:562190/93‑1 (MQ=255)
                        aTTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCg                                                                  >  2:5370931/1‑93 (MQ=255)
                        aTTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCg                                                                  <  2:6097902/93‑1 (MQ=255)
                        aTTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCg                                                                  >  1:2739805/1‑93 (MQ=255)
                        aTTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCg                                                                  >  1:3346468/1‑93 (MQ=255)
                         tttCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGa                                                                 <  1:2833469/93‑1 (MQ=255)
                         tttCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGa                                                                 <  1:310304/93‑1 (MQ=255)
                         tttCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGa                                                                 <  2:2801577/93‑1 (MQ=255)
                         tttCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGa                                                                 >  1:5490722/1‑93 (MQ=255)
                         tttCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGa                                                                 <  1:6020438/93‑1 (MQ=255)
                           tCTGCACTGTGGGAAGATGACGCCGATGGTATCGGCATCCCGTGGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATg                                                               <  2:6266786/93‑1 (MQ=255)
                            cTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGt                                                              >  1:3352414/1‑93 (MQ=255)
                             tGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGtt                                                             <  2:5904554/93‑1 (MQ=255)
                                aCCGTGGGAAGATGACGCCCAGTGTGTCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACg                                                          <  2:3837869/93‑1 (MQ=255)
                                 ccGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGa                                                         >  1:2224708/1‑93 (MQ=255)
                                  cGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGAc                                                        <  1:2908926/93‑1 (MQ=255)
                                  cGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGAGGTTACGAc                                                        >  1:4044493/1‑93 (MQ=255)
                                   gTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACa                                                       >  2:1460050/1‑93 (MQ=255)
                                    tGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACaa                                                      >  2:5477654/1‑93 (MQ=255)
                                     gggAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACaaa                                                     <  2:4300664/93‑1 (MQ=255)
                                     gggAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACaaa                                                     <  2:34036/93‑1 (MQ=255)
                                      ggAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACCAAt                                                    >  2:5421241/1‑93 (MQ=255)
                                      ggAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAAt                                                    <  2:4112029/93‑1 (MQ=255)
                                        aaGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGa                                                  <  1:1430825/93‑1 (MQ=255)
                                         aGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAt                                                 >  1:3931193/1‑93 (MQ=255)
                                          gATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAt‑t                                               >  2:44441/1‑93 (MQ=255)
                                          gATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAt‑t                                               >  1:6186382/1‑93 (MQ=255)
                                           aTGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAt‑tt                                              <  1:379441/93‑1 (MQ=255)
                                             gACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑cc                                           <  2:5181610/93‑1 (MQ=255)
                                             gACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑cc                                           >  1:5887840/1‑93 (MQ=255)
                                              aCGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑ccc                                          <  2:5294315/93‑1 (MQ=255)
                                              aCGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑ccc                                          <  2:3218316/93‑1 (MQ=255)
                                                gCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑ccccc                                        <  2:2955391/93‑1 (MQ=255)
                                                gCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑ccccc                                        <  2:5232357/93‑1 (MQ=255)
                                                 cccAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑cccccc                                       >  2:3636467/1‑92 (MQ=255)
                                                   cAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑ccccccat                                     >  1:3025059/1‑90 (MQ=255)
                                                   cAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑ccccccat                                     >  2:1288/1‑90 (MQ=255)
                                                    aGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑ccccccatt                                    >  1:3549438/1‑89 (MQ=255)
                                                    aGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑ccccccatt                                    <  2:369879/93‑5 (MQ=255)
                                                      tgtATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCCCCCATTTc                                   >  1:4418827/1‑93 (MQ=255)
                                                         aTCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCCCCCATTTCATg                                <  1:6264046/93‑1 (MQ=255)
                                                            ggCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCCCCCATTTCATGaaa                             <  1:5934855/93‑1 (MQ=255)
                                                            ggCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCCCCCATTTCATGaaa                             >  1:4199403/1‑93 (MQ=255)
                                                            ggCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCCCCCATTTCATGaaa                             <  2:811852/93‑1 (MQ=255)
                                                             gCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCCCCCATTTCATGAAAt                            <  1:3282575/93‑1 (MQ=255)
                                                              cATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCCCCCATTTCATGAAATg                           <  2:2450193/93‑1 (MQ=255)
                                                              cATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCCCCCATTTCATGAAATg                           <  2:463025/93‑1 (MQ=255)
                                                               aTCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCCCCCATTTCATGAAATgg                          <  2:3268894/93‑1 (MQ=255)
                                                               aTCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCCCCCATTTCATGAAATgg                          >  2:3763313/1‑93 (MQ=255)
                                                                tCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCCCCCATTTCATGAAATGGc                         <  1:364800/93‑1 (MQ=255)
                                                                tCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCCCCCATTTCATGAAATGGc                         >  1:749460/1‑93 (MQ=255)
                                                                tCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCCCCCATTTCATGAAATGGc                         >  1:4869263/1‑93 (MQ=255)
                                                                 cccGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCCCCCATTTCATGAAATGGCg                        <  2:4087481/93‑1 (MQ=255)
                                                                  ccGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCCCCCATTTCATGAAATGGCgg                       >  1:5073642/1‑93 (MQ=255)
                                                                  ccGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCCCCCATTTCATGAAATGGCgg                       <  2:114236/93‑1 (MQ=255)
                                                                  ccGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCCCCCATTTCATGAAATGGCgg                       >  1:1648339/1‑93 (MQ=255)
                                                                    gTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCCCCCATTTCATGAAATGGCGGgc                     >  2:1737740/1‑93 (MQ=255)
                                                                     ttGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCCCCCATTTCATGAAATGGCGGgct                    >  1:1821587/1‑93 (MQ=255)
                                                                     ttGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCCCCCATTTCATGAAATGGCGGgct                    >  2:6240284/1‑93 (MQ=255)
                                                                       gcgcAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGAGTCGATGTTACGACAAATGAT‑TTCCCCCCATTTCATGAAATGGCGGgctgc                  <  2:2425947/93‑1 (MQ=255)
                                                                            gTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCCCCCATTTCATGAAATGGCGGGctgctggca             >  2:163007/1‑90 (MQ=255)
                                                                             tGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCCCCCATTTCATGAAATGGCGGGCTGCTGcaca            <  1:1095395/93‑1 (MQ=255)
                                                                             tGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCCCCCATTTCATGAAATGGCGGGCTGCTGcaca            >  1:423688/1‑93 (MQ=255)
                                                                              gCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCCCCCATTTCATGAAATGGCGGGCTGCTGCACAt           >  1:4588226/1‑93 (MQ=255)
                                                                              gCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCCCCCATTTCATGAAATGGCGGGCTGCTGCACAt           <  1:6072359/93‑1 (MQ=255)
                                                                               cGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATg          <  1:2978395/93‑1 (MQ=255)
                                                                                gAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATgg         <  2:4174758/93‑1 (MQ=255)
                                                                                 aaCAGTGATGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATCGC‑CCCCCATTTCGTGATAGGGCGGGCTGCTCCACAGggc        <  2:2367406/93‑1 (MQ=255)
                                                                                 aaCAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATggc        <  1:3158878/93‑1 (MQ=255)
                                                                                 aaCAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATggc        >  2:158709/1‑93 (MQ=255)
                                                                                 aaCAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATggc        <  1:4748689/93‑1 (MQ=255)
                                                                                 aaCAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATagc        >  1:4501016/1‑93 (MQ=255)
                                                                                  aCAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATggcg       <  2:5801912/93‑1 (MQ=255)
                                                                                   cAGCGAAGCATGCGATTCGTTGAGTACAGATTCCATGTTACGACAAATGAT‑TTCCCCCCATTTCATGAAATGGCGGGCTGCTGGACATggcgg      <  2:5932967/93‑1 (MQ=255)
                                                                                     gCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGgcggcg    >  1:1585793/1‑93 (MQ=255)
                                                                                      cGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCga   <  1:3877346/93‑1 (MQ=255)
                                                                                          |                                                                                          
GCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑CCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAG  >  NC_000913/1562796‑1562974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTCGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTT‑CCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAAT  >  NC_000913/1562793‑1562977
                                                                                             |                                                                                            
GTCGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAG                                                                                              <  SRR4302533.2407540/94‑1 (MQ=60)
GTCGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAG                                                                                              <  SRR4302533.4429435/94‑1 (MQ=60)
GTCGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAG                                                                                              <  SRR4302533.1627469/94‑1 (MQ=60)
GTCGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAG                                                                                              <  SRR4302533.3543146/94‑1 (MQ=60)
GTCGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGCATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAG                                                                                              <  SRR4302533.1732305/94‑1 (MQ=60)
 TCGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGC                                                                                             >  SRR4302533.2283088/1‑94 (MQ=60)
 TCGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGC                                                                                             <  SRR4302533.34315/94‑1 (MQ=60)
  CGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCA                                                                                            >  SRR4302533.1475363/1‑94 (MQ=60)
   GCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCAT                                                                                           <  SRR4302533.5830171/94‑1 (MQ=60)
   GCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCAT                                                                                           <  SRR4302533.2502598/94‑1 (MQ=60)
    CTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATG                                                                                          >  SRR4302533.3525599/1‑94 (MQ=60)
     TGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGC                                                                                         <  SRR4302533.3930896/94‑1 (MQ=60)
     TGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGC                                                                                         <  SRR4302533.3229581/94‑1 (MQ=60)
      GACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCG                                                                                        <  SRR4302533.3360416/94‑1 (MQ=60)
      GACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCG                                                                                        >  SRR4302533.1939243/1‑94 (MQ=60)
      GACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCG                                                                                        >  SRR4302533.973166/1‑94 (MQ=60)
       ACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCTAACAGCGAAGCATGCGA                                                                                       >  SRR4302533.3571668/1‑94 (MQ=60)
       ACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGA                                                                                       >  SRR4302533.2435643/1‑94 (MQ=60)
        CCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGAT                                                                                      >  SRR4302533.765777/1‑94 (MQ=60)
         CAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATT                                                                                     <  SRR4302533.265213/94‑1 (MQ=60)
          AGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTC                                                                                    <  SRR4302533.2615200/94‑1 (MQ=60)
            CTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCTGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGT                                                                                  >  SRR4302533.3976689/1‑94 (MQ=60)
            CTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGT                                                                                  >  SRR4302533.3525837/1‑94 (MQ=60)
            CTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGT                                                                                  >  SRR4302533.967826/1‑94 (MQ=60)
            CTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGT                                                                                  >  SRR4302533.2538330/1‑94 (MQ=60)
            CTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGT                                                                                  >  SRR4302533.1197476/1‑94 (MQ=60)
               TGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGCTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGA                                                                               >  SRR4302533.4598758/1‑94 (MQ=60)
                GCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAG                                                                              <  SRR4302533.6189926/94‑1 (MQ=60)
                 CGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGT                                                                             <  SRR4302533.2687989/94‑1 (MQ=60)
                 CGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGT                                                                             >  SRR4302533.5512330/1‑94 (MQ=60)
                 CGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGT                                                                             >  SRR4302533.1964073/1‑94 (MQ=60)
                   CATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTAC                                                                           <  SRR4302533.3141229/94‑1 (MQ=60)
                    ATTTTGAATTTCTACACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACA                                                                          <  SRR4302533.3622986/94‑1 (MQ=60)
                      TTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGA                                                                        >  SRR4302533.927266/1‑94 (MQ=60)
                      TTTGAATGTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGA                                                                        <  SRR4302533.3220030/94‑1 (MQ=60)
                       TTGAATTTCTGCACCGTGGGAAGATGTCTGCCAGTGTAGGGGCATTGCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGAT                                                                       <  SRR4302533.3069632/94‑1 (MQ=60)
                       TTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGAT                                                                       <  SRR4302533.3277463/94‑1 (MQ=60)
                       TTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGAT                                                                       <  SRR4302533.796520/94‑1 (MQ=60)
                       TTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGAT                                                                       <  SRR4302533.796519/94‑1 (MQ=60)
                       TTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGAT                                                                       <  SRR4302533.2268571/94‑1 (MQ=60)
                         GAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTC                                                                     >  SRR4302533.696895/1‑94 (MQ=60)
                          AATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGGACAGAGAAGCATGCGATTCGTTGAGTACAGATTCG                                                                    >  SRR4302533.2756603/1‑94 (MQ=60)
                          AATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATNCGATTCGTTGAGTACAGATNCG                                                                    >  SRR4302533.4966709/1‑94 (MQ=60)
                          AATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCG                                                                    <  SRR4302533.565523/94‑1 (MQ=60)
                          AATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCG                                                                    >  SRR4302533.3367143/1‑94 (MQ=60)
                          AATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCG                                                                    >  SRR4302533.2756602/1‑94 (MQ=60)
                           ATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGA                                                                   >  SRR4302533.5523822/1‑94 (MQ=60)
                            TTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGAT                                                                  <  SRR4302533.312203/94‑1 (MQ=60)
                            TTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGAT                                                                  <  SRR4302533.2850912/94‑1 (MQ=60)
                            TTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGAT                                                                  <  SRR4302533.6056919/94‑1 (MQ=60)
                             TTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATG                                                                 >  SRR4302533.3196691/1‑94 (MQ=60)
                              TCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGT                                                                >  SRR4302533.3373123/1‑94 (MQ=60)
                               CTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTT                                                               >  SRR4302533.2409319/1‑94 (MQ=60)
                                 GCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTAC                                                             <  SRR4302533.5541149/94‑1 (MQ=60)
                                   ACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGA                                                           >  SRR4302533.1625809/1‑94 (MQ=60)
                                   ACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGA                                                           >  SRR4302533.4738775/1‑94 (MQ=60)
                                   ACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGA                                                           >  SRR4302533.2238444/1‑94 (MQ=60)
                                    CCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGAGGTTACGAC                                                          >  SRR4302533.4069110/1‑94 (MQ=60)
                                     CGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACGGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACA                                                         >  SRR4302533.3634050/1‑94 (MQ=60)
                                     CGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACA                                                         <  SRR4302533.2926783/94‑1 (MQ=60)
                                      GTGGGAAGATGACGCCCAGTGTATCGGCGTCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAA                                                        >  SRR4302533.4480755/1‑94 (MQ=60)
                                        GGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAAT                                                      <  SRR4302533.3368630/94‑1 (MQ=60)
                                        GGGAAGATGACGACCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAAT                                                      <  SRR4302533.2873225/94‑1 (MQ=60)
                                           AAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT                                                   >  SRR4302533.3955193/1‑94 (MQ=60)
                                           AAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT                                                   <  SRR4302533.1439474/94‑1 (MQ=60)
                                           AAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT                                                   <  SRR4302533.458845/94‑1 (MQ=60)
                                            AGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT                                                  >  SRR4302533.6223517/1‑94 (MQ=60)
                                            AGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATT                                                  <  SRR4302533.5987883/94‑1 (MQ=60)
                                             GATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTT                                                 >  SRR4302533.3542977/1‑94 (MQ=60)
                                             GATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTT                                                 <  SRR4302533.1647559/94‑1 (MQ=60)
                                              ATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTT‑C                                               <  SRR4302533.381727/94‑1 (MQ=60)
                                               TGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTT‑CC                                              >  SRR4302533.5923412/1‑94 (MQ=60)
                                                GACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTT‑CCC                                             >  SRR4302533.2803749/1‑94 (MQ=60)
                                                     CCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTT‑CCCCCcat                                        >  SRR4302533.3043565/1‑91 (MQ=60)
                                                      CAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATT                                        >  SRR4302533.3571307/1‑94 (MQ=60)
                                                      CAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATT                                        >  SRR4302533.3000355/1‑94 (MQ=60)
                                                       AGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTT                                       >  SRR4302533.2606175/1‑94 (MQ=60)
                                                        GTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTC                                      >  SRR4302533.2986875/1‑94 (MQ=60)
                                                        GTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTC                                      >  SRR4302533.4445543/1‑94 (MQ=60)
                                                          GTATCGGCATCCGGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCAT                                    <  SRR4302533.4259257/94‑1 (MQ=60)
                                                          GTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCAT                                    >  SRR4302533.4171660/1‑94 (MQ=60)
                                                           TATCGCCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATG                                   <  SRR4302533.1139481/94‑1 (MQ=60)
                                                            ATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGA                                  <  SRR4302533.6301486/94‑1 (MQ=60)
                                                            ATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGA                                  <  SRR4302533.5411511/94‑1 (MQ=60)
                                                              CGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAA                                >  SRR4302533.4224816/1‑94 (MQ=60)
                                                               GGCATCCCGTTGCGCAGTGCGAACAGCGAGGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAAT                               <  SRR4302533.6098468/94‑1 (MQ=60)
                                                               GGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAAT                               <  SRR4302533.5970735/94‑1 (MQ=60)
                                                               CGCATCCCGTTGCGCAGTGCGAACCGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAAT                               <  SRR4302533.1391130/94‑1 (MQ=60)
                                                                GCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAATG                              <  SRR4302533.3302839/94‑1 (MQ=60)
                                                                 CATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAATGG                             <  SRR4302533.4338571/94‑1 (MQ=60)
                                                                  ATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAATGGC                            >  SRR4302533.75586/1‑94 (MQ=60)
                                                                  ATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAATGGC                            >  SRR4302533.753875/1‑94 (MQ=60)
                                                                  ATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAATGGC                            >  SRR4302533.4898766/1‑94 (MQ=60)
                                                                  ATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAATGGC                            <  SRR4302533.5758779/94‑1 (MQ=60)
                                                                   TCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAATGGCG                           <  SRR4302533.367013/94‑1 (MQ=60)
                                                                    CCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAATGGCGG                          >  SRR4302533.1658325/1‑94 (MQ=60)
                                                                    CCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAATGGCGG                          >  SRR4302533.4217192/1‑94 (MQ=60)
                                                                    CCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAATGGCGG                          >  SRR4302533.5104309/1‑94 (MQ=60)
                                                                     CCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAATGGCGGG                         <  SRR4302533.5963228/94‑1 (MQ=60)
                                                                     CCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAATGGCGGG                         <  SRR4302533.302365/94‑1 (MQ=60)
                                                                       GTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAATGGCGGGCT                       >  SRR4302533.1832712/1‑94 (MQ=60)
                                                                          GCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAATGGCGGGCTGCT                    <  SRR4302533.5425238/94‑1 (MQ=60)
                                                                            GCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAATGGCGGGCTGCTGC                  >  SRR4302533.4837692/1‑94 (MQ=60)
                                                                             CAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAATGGCGGGCTGCTGCC                 >  SRR4302533.377809/1‑94 (MQ=60)
                                                                             CAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAATGGCGGGCTGCTGCA                 >  SRR4302533.3848033/1‑94 (MQ=60)
                                                                               GTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAATGGCGGGCTGCTGCACA               >  SRR4302533.426223/1‑94 (MQ=60)
                                                                               GTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAATGGCGGGCTGCTGCACA               >  SRR4302533.5520950/1‑94 (MQ=60)
                                                                                TGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAATGGCGGGCTGCTGCACAT              <  SRR4302533.1101879/94‑1 (MQ=60)
                                                                                TGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAATGGCGGGCTGCTGCACAT              >  SRR4302533.4616017/1‑94 (MQ=60)
                                                                                TGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAATGGCGGGCTGCTGCACAT              >  SRR4302533.6133157/1‑94 (MQ=60)
                                                                                 GCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATG             <  SRR4302533.6109095/94‑1 (MQ=60)
                                                                                  CGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGG            <  SRR4302533.2996618/94‑1 (MQ=60)
                                                                                   GAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATAGC           >  SRR4302533.4528280/1‑94 (MQ=60)
                                                                                    AACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCG          <  SRR4302533.4777481/94‑1 (MQ=60)
                                                                                    AACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCG          <  SRR4302533.3178318/94‑1 (MQ=60)
                                                                                     ACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGG         >  SRR4302533.3500978/1‑94 (MQ=60)
                                                                                     ACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGG         >  SRR4302533.3764697/1‑94 (MQ=60)
                                                                                      CAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGC        <  SRR4302533.6280987/94‑1 (MQ=60)
                                                                                       AGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCG       >  SRR4302533.2178033/1‑94 (MQ=60)
                                                                                       AGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCG       <  SRR4302533.1752636/94‑1 (MQ=60)
                                                                                       AGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCG       >  SRR4302533.1595348/1‑94 (MQ=60)
                                                                                         CGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAG     <  SRR4302533.3901009/94‑1 (MQ=60)
                                                                                          GAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGA    >  SRR4302533.3372812/1‑94 (MQ=60)
                                                                                            AGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAAT  <  SRR4302533.2173241/94‑1 (MQ=60)
                                                                                             |                                                                                            
GTCGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTT‑CCCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAAT  >  NC_000913/1562793‑1562977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑