Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,562,886 A→G V299A (GTT→GCT)  b1489 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,562,8860AG99.6% 861.7 / ‑5.8 267V299A (GTT→GCT) b1489hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (132/134);  total (132/135)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 272 total aligned reads displayed.

GCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑CC‑CCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAG  >  NC_000913/1562796‑1562974
                                                                                          |                                                                                           
gCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCa                                                                                           <  1:5622097/93‑1 (MQ=255)
gCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCa                                                                                           >  2:1483560/1‑93 (MQ=255)
gCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCa                                                                                           <  2:6451960/93‑1 (MQ=255)
ccTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCa                                                                                           <  2:8796821/92‑1 (MQ=255)
 cTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCAt                                                                                          <  1:1618189/93‑1 (MQ=255)
  tGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATg                                                                                         >  2:3899653/1‑93 (MQ=255)
  tGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATg                                                                                         <  1:1572948/93‑1 (MQ=255)
  tGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATg                                                                                         >  1:2312069/1‑93 (MQ=255)
   gACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGc                                                                                        >  2:5797746/1‑93 (MQ=255)
   gACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGc                                                                                        <  1:4565748/93‑1 (MQ=255)
      cAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGAt                                                                                     >  1:7311209/1‑93 (MQ=255)
       aGCTATGCGCATTTTGAATTACTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGAtt                                                                                    <  2:9271900/93‑1 (MQ=255)
        gCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTc                                                                                   >  1:7887727/1‑93 (MQ=255)
         cTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCg                                                                                  <  1:8733873/93‑1 (MQ=255)
          tttGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGGTTCGt                                                                                 >  1:2455530/1‑93 (MQ=255)
          tttGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGt                                                                                 >  2:5924128/1‑93 (MQ=255)
           ttGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGtt                                                                                <  2:6793768/93‑1 (MQ=255)
            tGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTg                                                                               <  1:9609158/93‑1 (MQ=255)
               gcATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGt                                                                            <  1:5409074/93‑1 (MQ=255)
               gcATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGt                                                                            <  1:4152431/93‑1 (MQ=255)
               ccATTTTTAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGt                                                                            <  2:2857058/92‑1 (MQ=255)
                cATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTa                                                                           <  1:3803993/93‑1 (MQ=255)
                 aTTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTAc                                                                          <  2:7164835/93‑1 (MQ=255)
                  ttttGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACa                                                                         >  2:5359913/1‑93 (MQ=255)
                  ttttGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACa                                                                         >  1:9209882/1‑93 (MQ=255)
                   tttGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAg                                                                        >  1:4320281/1‑93 (MQ=255)
                   tttGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAg                                                                        <  1:3560399/93‑1 (MQ=255)
                   tttGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAg                                                                        <  2:6712797/93‑1 (MQ=255)
                    ttGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGa                                                                       <  2:7537219/93‑1 (MQ=255)
                    ttGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGa                                                                       >  1:7589280/1‑93 (MQ=255)
                    ttGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGa                                                                       <  2:3746048/93‑1 (MQ=255)
                      gAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGAtt                                                                     <  1:3368829/93‑1 (MQ=255)
                      gAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGAtt                                                                     <  2:5422755/93‑1 (MQ=255)
                      gAATCTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTGGTTGAGTACAGAtt                                                                     <  2:1705206/93‑1 (MQ=255)
                       aaTTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTc                                                                    >  1:9037753/1‑93 (MQ=255)
                        aTTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCg                                                                   >  1:6570539/1‑93 (MQ=255)
                        aTTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCg                                                                   >  1:4980671/1‑93 (MQ=255)
                         tttCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGa                                                                  <  1:6887155/93‑1 (MQ=255)
                         tttCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGa                                                                  >  2:8646211/1‑93 (MQ=255)
                         tttCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGa                                                                  <  1:252287/93‑1 (MQ=255)
                          ttCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGAt                                                                 >  1:2689691/1‑93 (MQ=255)
                          ttCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGAt                                                                 <  1:8808148/93‑1 (MQ=255)
                           tCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATg                                                                <  1:4132586/93‑1 (MQ=255)
                             tGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGtt                                                              >  2:6629316/1‑93 (MQ=255)
                               cACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTag                                                            >  2:2991032/1‑92 (MQ=255)
                               cACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTAc                                                            <  1:8359422/93‑1 (MQ=255)
                                  cGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGAc                                                         <  1:1845772/93‑1 (MQ=255)
                                  cGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGAc                                                         <  2:2071588/93‑1 (MQ=255)
                                  cGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGAc                                                         <  1:7059581/93‑1 (MQ=255)
                                   gTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACa                                                        >  2:9601395/1‑93 (MQ=255)
                                    tGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACaa                                                       <  2:117581/93‑1 (MQ=255)
                                     gggAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACaaa                                                      <  1:805171/93‑1 (MQ=255)
                                      ggAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAAt                                                     >  1:6909306/1‑93 (MQ=255)
                                        aaGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGa                                                   <  1:8762435/93‑1 (MQ=255)
                                         aGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGCAGCATGCGATTCGTTGAGTACAGATTCGATGCTACGACAAATGAt                                                  >  1:4052378/1‑93 (MQ=255)
                                          gATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAt‑t                                                <  2:5202359/93‑1 (MQ=255)
                                          gATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAt‑t                                                >  1:5037761/1‑93 (MQ=255)
                                           aTGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAt‑tt                                               >  1:378336/1‑93 (MQ=255)
                                            tGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑c                                             <  1:1014557/93‑1 (MQ=255)
                                            tGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑c                                             >  1:2135513/1‑93 (MQ=255)
                                             gACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑cc                                            >  2:8403748/1‑93 (MQ=255)
                                              aCGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑cc‑c                                          >  2:7487621/1‑93 (MQ=255)
                                                gCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑cc‑ccc                                        <  2:187006/93‑1 (MQ=255)
                                                 cccAGTGTATCGGCATCCCGTTGCGCAGTGCGCACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑cc‑cccc                                       >  2:4885507/1‑92 (MQ=255)
                                                   cAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑CC‑CCCaat                                     >  1:4644158/1‑91 (MQ=255)
                                                      tgtATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTc                                   <  1:7148571/93‑1 (MQ=255)
                                                      tgtATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTc                                   >  1:6311351/1‑93 (MQ=255)
                                                        tATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCAt                                 >  2:3058563/1‑93 (MQ=255)
                                                         aTCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATg                                >  2:6152941/1‑93 (MQ=255)
                                                           cGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGaa                              >  1:6455968/1‑93 (MQ=255)
                                                             gCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAAt                            >  2:631295/1‑93 (MQ=255)
                                                             gCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAAt                            <  2:9796664/93‑1 (MQ=255)
                                                             gCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAAt                            >  2:3175329/1‑93 (MQ=255)
                                                             gCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAAt                            >  1:5254790/1‑93 (MQ=255)
                                                               aTCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑CCACCCATTTCATGAAATgg                          >  2:8113659/1‑93 (MQ=255)
                                                                tCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAATGGc                         >  2:7604284/1‑93 (MQ=255)
                                                                tCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAATGGc                         >  1:5052059/1‑93 (MQ=255)
                                                                tCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAATGGc                         >  1:9655083/1‑93 (MQ=255)
                                                                tCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAATGGc                         >  2:1616111/1‑93 (MQ=255)
                                                                tCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAATGGc                         >  2:1229284/1‑93 (MQ=255)
                                                                tCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAATGGc                         <  1:9968902/93‑1 (MQ=255)
                                                                tCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑CC‑CCCATTTCATGAAATGGCg                        <  2:3477613/93‑1 (MQ=255)
                                                                 cccGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAATGGCg                        <  1:83146/93‑1 (MQ=255)
                                                                   cGTGGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑CC‑CCCATTTCATGAAATGGCGGgc                     <  2:1305785/93‑1 (MQ=255)
                                                                    gTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAATGGCGGgc                     <  1:275849/93‑1 (MQ=255)
                                                                         gcAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAATGGCGGGctgctg                <  1:7781197/93‑1 (MQ=255)
                                                                          cAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATGTC‑CC‑CCCATTTCATGAAATGGCGGGCtgatgc               >  1:588989/1‑93 (MQ=255)
                                                                             tGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAACTGGCGGGCTGCTGccca            >  2:2606729/1‑93 (MQ=255)
                                                                             tGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAATGGCGGGCTGCTGcaca            >  1:9339607/1‑93 (MQ=255)
                                                                              gCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAATGGCGGGCTGCTGCACAt           <  2:9098153/93‑1 (MQ=255)
                                                                              gCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAATGGCGGGCTGCTGCACAt           <  1:1301859/93‑1 (MQ=255)
                                                                              gCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAATGGCGGGCTGCTGCACAt           <  1:8627655/93‑1 (MQ=255)
                                                                                gAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATTTTACGACAAATGAT‑TT‑CCTCCCACTTCATGAAATGGCGGTCTTCTGCACCTgg         >  2:7073991/1‑93 (MQ=255)
                                                                                gAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAATGGCGGGCTGCTGCACATgg         >  2:2244908/1‑93 (MQ=255)
                                                                                gAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAATGGCGGGCTGCTGCACATgg         <  2:4636682/93‑1 (MQ=255)
                                                                                 aaCAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAATGGCGGGCTGCTGCACATggc        >  2:2496246/1‑93 (MQ=255)
                                                                                 aaCAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAATGGCGGGCTGCTGCACATggc        <  2:4245986/93‑1 (MQ=255)
                                                                                  aCAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAATGGCGGGCTGCTGCACATggcg       >  1:2917401/1‑93 (MQ=255)
                                                                                    aGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAATGGCGGGCTGCTGCACATggcggc     <  2:4053112/93‑1 (MQ=255)
                                                                                     gCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑CC‑CCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCga   <  2:5957030/93‑1 (MQ=255)
                                                                                          |                                                                                           
GCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑CC‑CCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAG  >  NC_000913/1562796‑1562974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTCGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑C‑CCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATAT  >  NC_000913/1562793‑1562979
                                                                                             |                                                                                                
GTCGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAG                                                                                                  <  SRR4302540.8130447/94‑1 (MQ=60)
 TCGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGC                                                                                                 <  SRR4302540.2986745/94‑1 (MQ=60)
 TCGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGC                                                                                                 <  SRR4302540.7641468/94‑1 (MQ=60)
  CGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCA                                                                                                >  SRR4302540.1591525/1‑94 (MQ=60)
   GCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCAT                                                                                               <  SRR4302540.5683957/94‑1 (MQ=60)
   GCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCAT                                                                                               <  SRR4302540.8173237/94‑1 (MQ=60)
   GCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCAT                                                                                               <  SRR4302540.6793295/94‑1 (MQ=60)
   GCTGACCAGCTTTGCGCATTTTGAAGTTCTGCGCCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCAT                                                                                               <  SRR4302540.2169011/94‑1 (MQ=60)
    CTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATG                                                                                              >  SRR4302540.2336955/1‑94 (MQ=60)
    CTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATG                                                                                              <  SRR4302540.1635934/94‑1 (MQ=60)
     TGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGC                                                                                             >  SRR4302540.430584/1‑94 (MQ=60)
     TGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGC                                                                                             <  SRR4302540.1590176/94‑1 (MQ=60)
      GACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCG                                                                                            <  SRR4302540.4615051/94‑1 (MQ=60)
       ACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGA                                                                                           <  SRR4302540.3442046/94‑1 (MQ=60)
        CCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGAT                                                                                          <  SRR4302540.1997754/94‑1 (MQ=60)
        CCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGAT                                                                                          >  SRR4302540.7392836/1‑94 (MQ=60)
          AGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTC                                                                                        >  SRR4302540.7979797/1‑94 (MQ=60)
            CTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGGTTCGT                                                                                      >  SRR4302540.2481956/1‑94 (MQ=60)
            CTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGT                                                                                      <  SRR4302540.8835290/94‑1 (MQ=60)
            CTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGT                                                                                      >  SRR4302540.9730657/1‑94 (MQ=60)
               TGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGA                                                                                   <  SRR4302540.9722466/94‑1 (MQ=60)
               TGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGA                                                                                   <  SRR4302540.9722467/94‑1 (MQ=60)
                 CGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGT                                                                                 >  SRR4302540.3748270/1‑94 (MQ=60)
                  GCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTA                                                                                <  SRR4302540.5468551/94‑1 (MQ=60)
                  GCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTA                                                                                >  SRR4302540.8733181/1‑94 (MQ=60)
                  GCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTA                                                                                <  SRR4302540.4197407/94‑1 (MQ=60)
                   CATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTAC                                                                               >  SRR4302540.7931402/1‑94 (MQ=60)
                   CATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTAC                                                                               <  SRR4302540.9375663/94‑1 (MQ=60)
                   CATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTAC                                                                               <  SRR4302540.3845427/94‑1 (MQ=60)
                   CATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTAC                                                                               <  SRR4302540.7156883/94‑1 (MQ=60)
                    ATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACA                                                                              >  SRR4302540.9320390/1‑94 (MQ=60)
                     TTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAG                                                                             <  SRR4302540.7588179/94‑1 (MQ=60)
                     TTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAG                                                                             >  SRR4302540.4367315/1‑94 (MQ=60)
                      TTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGA                                                                            >  SRR4302540.7674517/1‑94 (MQ=60)
                      TTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGA                                                                            <  SRR4302540.3599292/94‑1 (MQ=60)
                       TTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGAT                                                                           <  SRR4302540.8243177/94‑1 (MQ=60)
                       TTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGAT                                                                           <  SRR4302540.8994053/94‑1 (MQ=60)
                       TTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGAT                                                                           <  SRR4302540.1933712/94‑1 (MQ=60)
                        TGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATT                                                                          <  SRR4302540.158356/94‑1 (MQ=60)
                         TAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTC                                                                         <  SRR4302540.5374209/94‑1 (MQ=60)
                         GAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTC                                                                         >  SRR4302540.9146234/1‑94 (MQ=60)
                         GAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTC                                                                         <  SRR4302540.3405668/94‑1 (MQ=60)
                          AATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCG                                                                        >  SRR4302540.6643342/1‑94 (MQ=60)
                          AATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCG                                                                        >  SRR4302540.5034589/1‑94 (MQ=60)
                            TTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGAT                                                                      <  SRR4302540.6963506/94‑1 (MQ=60)
                            TTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGAT                                                                      <  SRR4302540.254727/94‑1 (MQ=60)
                            TTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGAT                                                                      >  SRR4302540.2718751/1‑94 (MQ=60)
                             TTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATG                                                                     <  SRR4302540.8909981/94‑1 (MQ=60)
                             TTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATG                                                                     <  SRR4302540.5716049/94‑1 (MQ=60)
                              TCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGT                                                                    <  SRR4302540.4177286/94‑1 (MQ=60)
                                  CACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACG                                                                <  SRR4302540.8456428/94‑1 (MQ=60)
                                   ACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGA                                                               >  SRR4302540.4196652/1‑94 (MQ=60)
                                     CGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACA                                                             <  SRR4302540.1865997/94‑1 (MQ=60)
                                     CGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACA                                                             <  SRR4302540.7138095/94‑1 (MQ=60)
                                     CGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACA                                                             <  SRR4302540.3844329/94‑1 (MQ=60)
                                        GGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAAT                                                          >  SRR4302540.6986166/1‑94 (MQ=60)
                                        GGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAAT                                                          <  SRR4302540.813724/94‑1 (MQ=60)
                                         GGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATG                                                         >  SRR4302540.2605234/1‑94 (MQ=60)
                                          GAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGA                                                        <  SRR4302540.4022104/94‑1 (MQ=60)
                                           AAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGCAGCATGCGATTCGTTGAGTACAGATTCGATGCTACGACAAATGAT                                                       >  SRR4302540.4096069/1‑94 (MQ=60)
                                           AAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT                                                       >  SRR4302540.2823590/1‑94 (MQ=60)
                                           AAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT                                                       >  SRR4302540.3141323/1‑94 (MQ=60)
                                           AAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT                                                       <  SRR4302540.8864028/94‑1 (MQ=60)
                                           AAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT                                                       >  SRR4302540.4096070/1‑94 (MQ=60)
                                           AAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT                                                       >  SRR4302540.2782334/1‑94 (MQ=60)
                                            AGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T                                                     <  SRR4302540.4481441/94‑1 (MQ=60)
                                            AGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T                                                     >  SRR4302540.5092256/1‑94 (MQ=60)
                                            AGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑T                                                     <  SRR4302540.402390/94‑1 (MQ=60)
                                             GATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT                                                    <  SRR4302540.8433577/94‑1 (MQ=60)
                                             GATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT                                                    <  SRR4302540.77637/94‑1 (MQ=60)
                                             GATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT                                                    >  SRR4302540.382250/1‑94 (MQ=60)
                                             AATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT                                                    <  SRR4302540.8854505/94‑1 (MQ=60)
                                              ATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑C                                                  >  SRR4302540.2158440/1‑94 (MQ=60)
                                              ATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑C                                                  >  SRR4302540.8551114/1‑94 (MQ=60)
                                               TGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑C‑C                                                >  SRR4302540.1259032/1‑94 (MQ=60)
                                               TGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑C‑C                                                <  SRR4302540.1024842/94‑1 (MQ=60)
                                                  CGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑C‑CCCC                                             <  SRR4302540.27552/94‑1 (MQ=60)
                                                  CGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑C‑CCCC                                             <  SRR4302540.4673125/94‑1 (MQ=60)
                                                  CGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑C‑CCCC                                             >  SRR4302540.6615783/1‑94 (MQ=60)
                                                  CGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑C‑CCCC                                             >  SRR4302540.6615782/1‑94 (MQ=60)
                                                   GCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑C‑CCCCC                                            >  SRR4302540.7026961/1‑94 (MQ=60)
                                                   GCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑C‑CCCCC                                            >  SRR4302540.3229546/1‑94 (MQ=60)
                                                     CCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGATTACGGATTCGATGTTACGACAAATGAT‑TT‑C‑CCCCcat                                          >  SRR4302540.4693869/1‑91 (MQ=60)
                                                     CCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑C‑CCCCcat                                          <  SRR4302540.5184238/94‑4 (MQ=60)
                                                     CCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑C‑CCCCAAT                                          >  SRR4302540.4693870/1‑94 (MQ=60)
                                                      CAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATT                                          <  SRR4302540.5745695/94‑1 (MQ=60)
                                                      CAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATT                                          <  SRR4302540.9270833/94‑1 (MQ=60)
                                                      CAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATT                                          <  SRR4302540.2466847/94‑1 (MQ=60)
                                                      CAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑C‑CCCCcact                                         >  SRR4302540.7959014/1‑90 (MQ=60)
                                                       AGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTT                                         <  SRR4302540.7989941/94‑1 (MQ=60)
                                                        GTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTC                                        >  SRR4302540.6381627/1‑94 (MQ=60)
                                                        GTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑C‑CCCCATTTCA                                       >  SRR4302540.5073492/1‑94 (MQ=60)
                                                         TGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCA                                       <  SRR4302540.7228228/94‑1 (MQ=60)
                                                          GTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCAT                                      <  SRR4302540.5624531/94‑1 (MQ=60)
                                                           TATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATG                                     >  SRR4302540.6246690/1‑94 (MQ=60)
                                                             TCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGCTGTTACGACAACTGAT‑TTCC‑CCCCACTTCATGAA                                   >  SRR4302540.6527828/1‑94 (MQ=60)
                                                             TCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAA                                   >  SRR4302540.6527833/1‑94 (MQ=60)
                                                               GGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAAT                                 <  SRR4302540.2544830/94‑1 (MQ=60)
                                                               GGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAAT                                 <  SRR4302540.1604049/94‑1 (MQ=60)
                                                               GGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAAT                                 <  SRR4302540.7156261/94‑1 (MQ=60)
                                                               GGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAAT                                 >  SRR4302540.5312103/1‑94 (MQ=60)
                                                                GCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑C‑CCCCATTTCATGAAATGG                               <  SRR4302540.9294481/94‑1 (MQ=60)
                                                                 CATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGG                               >  SRR4302540.7472859/1‑94 (MQ=60)
                                                                 CATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGG                               >  SRR4302540.8843427/1‑94 (MQ=60)
                                                                  ATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGGC                              >  SRR4302540.5106675/1‑94 (MQ=60)
                                                                  ATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGGC                              >  SRR4302540.5106677/1‑94 (MQ=60)
                                                                  ATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGGC                              >  SRR4302540.4893387/1‑94 (MQ=60)
                                                                  ATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGGC                              >  SRR4302540.945113/1‑94 (MQ=60)
                                                                  ATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGGC                              >  SRR4302540.6940188/1‑94 (MQ=60)
                                                                  ATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGGC                              >  SRR4302540.9768754/1‑94 (MQ=60)
                                                                  ATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑C‑CCCCATTTCATGAAATGGCG                             >  SRR4302540.8444510/1‑94 (MQ=60)
                                                                   TCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGGCG                             <  SRR4302540.10085200/94‑1 (MQ=60)
                                                                    CCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGGCGG                            <  SRR4302540.649975/94‑1 (MQ=60)
                                                                    CCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGGCGG                            <  SRR4302540.84021/94‑1 (MQ=60)
                                                                    CCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGGCGG                            <  SRR4302540.4267323/94‑1 (MQ=60)
                                                                      CGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGGCGGGC                          <  SRR4302540.8795656/94‑1 (MQ=60)
                                                                       GTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGGCGGGCT                         <  SRR4302540.9391515/94‑1 (MQ=60)
                                                                       GTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGGCGGGCT                         <  SRR4302540.278563/94‑1 (MQ=60)
                                                                        TTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGGCGGGCTG                        <  SRR4302540.9964540/94‑1 (MQ=60)
                                                                        TTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGGCGGGCTG                        <  SRR4302540.1409688/94‑1 (MQ=60)
                                                                         TGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGGCGGGCTGC                       >  SRR4302540.2589932/1‑94 (MQ=60)
                                                                           CGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGGCGGGCTGCTG                     >  SRR4302540.217136/1‑94 (MQ=60)
                                                                           CGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGAAAAATGAT‑TTCC‑CCCCATTTCATGAAATGGCGGGCTGCTG                     >  SRR4302540.7233758/1‑94 (MQ=60)
                                                                            GCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATGTC‑C‑CCCCATTTCATGAAATGGCGGGCTGATGC                    >  SRR4302540.595339/1‑94 (MQ=60)
                                                                            GCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGGCGGGCTGCTGC                    <  SRR4302540.7871855/94‑1 (MQ=60)
                                                                            GCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGGCGGGCTGCTGC                    <  SRR4302540.7872152/94‑1 (MQ=60)
                                                                              AGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGGCGGGCTGCTGCAC                  >  SRR4302540.5558468/1‑94 (MQ=60)
                                                                              AGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGGCGGGCTGCTGCAC                  >  SRR4302540.8899152/1‑94 (MQ=60)
                                                                               GTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGGCGGGCTGCTGCACA                 >  SRR4302540.9450916/1‑94 (MQ=60)
                                                                                TGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGGCGGGCTGCTGCACAT                <  SRR4302540.5054199/94‑1 (MQ=60)
                                                                                TGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑C‑CCCCATTTCATGAAATGGCGGGCTGCTGCACATG               <  SRR4302540.5172225/94‑1 (MQ=60)
                                                                                 GCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGGCGGGCTGCTGCACATG               <  SRR4302540.8727869/94‑1 (MQ=60)
                                                                                 GCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGGCGGGCTGCTGCACATG               <  SRR4302540.1315356/94‑1 (MQ=60)
                                                                                  CGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGGCGGGCTGCTGCACATGG              >  SRR4302540.3514044/1‑94 (MQ=60)
                                                                                   GAAGAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGGCGGGCTGCTGCACATGGC             <  SRR4302540.1848068/94‑1 (MQ=60)
                                                                                   GAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGGCGGGCTGCTGCACATGGC             <  SRR4302540.4142928/94‑1 (MQ=60)
                                                                                   GAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGGCGGGCTGCTGCACATGGC             <  SRR4302540.7059299/94‑1 (MQ=60)
                                                                                   GAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGGCGGGCTGCTGCACATGGC             <  SRR4302540.2458538/94‑1 (MQ=60)
                                                                                    AACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCG            >  SRR4302540.2949329/1‑94 (MQ=60)
                                                                                    AACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑C‑CCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGG           <  SRR4302540.3599712/94‑1 (MQ=60)
                                                                                      CAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGC          <  SRR4302540.8894750/94‑1 (MQ=60)
                                                                                       AGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCG         <  SRR4302540.4386099/94‑1 (MQ=60)
                                                                                       AGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGGCGGGCTGCTGCACACGGCGGCG         >  SRR4302540.6985414/1‑94 (MQ=60)
                                                                                         CGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAG       >  SRR4302540.5106189/1‑94 (MQ=60)
                                                                                         CGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAG       <  SRR4302540.10001642/94‑1 (MQ=60)
                                                                                          GAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑CACCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGA      >  SRR4302540.7172430/1‑94 (MQ=60)
                                                                                           AAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAA     <  SRR4302540.1420491/94‑1 (MQ=60)
                                                                                           AAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCC‑CCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAA     >  SRR4302540.1289483/1‑94 (MQ=60)
                                                                                             GCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑C‑CCCCATTTCATGAAATTGCGGGCTGCTGCACATGGCGGCGACAATAT  >  SRR4302540.6724085/1‑94 (MQ=60)
                                                                                             |                                                                                                
GTCGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑C‑CCCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAGAATAT  >  NC_000913/1562793‑1562979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑